PMC:3589482 / 36072-38349 JSONTXT

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T26996","span":{"begin":2276,"end":2277},"obj":"."},{"id":"T26995","span":{"begin":2275,"end":2276},"obj":"-RRB-"},{"id":"T26994","span":{"begin":2270,"end":2275},"obj":"NNP"},{"id":"T26993","span":{"begin":2262,"end":2269},"obj":"JJ"},{"id":"T26992","span":{"begin":2260,"end":2261},"obj":","},{"id":"T26991","span":{"begin":2256,"end":2260},"obj":"CD"},{"id":"T26990","span":{"begin":2255,"end":2256},"obj":"RB"},{"id":"T26989","span":{"begin":2254,"end":2255},"obj":"VB"},{"id":"T26988","span":{"begin":2252,"end":2253},"obj":","},{"id":"T26987","span":{"begin":2251,"end":2252},"obj":"SYM"},{"id":"T26986","span":{"begin":2250,"end":2251},"obj":"-LRB-"},{"id":"T26985","span":{"begin":2248,"end":2249},"obj":"."},{"id":"T26984","span":{"begin":2238,"end":2248},"obj":"NN"},{"id":"T26983","span":{"begin":2235,"end":2237},"obj":"IN"},{"id":"T26982","span":{"begin":2225,"end":2234},"obj":"VBN"},{"id":"T26981","span":{"begin":2213,"end":2224},"obj":"NNS"},{"id":"T26980","span":{"begin":2201,"end":2212},"obj":"JJ"},{"id":"T26979","span":{"begin":2195,"end":2200},"obj":"CD"},{"id":"T26978","span":{"begin":2189,"end":2194},"obj":"JJS"},{"id":"T26977","span":{"begin":2186,"end":2188},"obj":"IN"},{"id":"T26976","span":{"begin":2183,"end":2185},"obj":"IN"},{"id":"T26975","span":{"begin":2179,"end":2182},"obj":"NNP"},{"id":"T26974","span":{"begin":2175,"end":2178},"obj":"CC"},{"id":"T26973","span":{"begin":2170,"end":2174},"obj":"NN"},{"id":"T26972","span":{"begin":2166,"end":2169},"obj":"DT"},{"id":"T26971","span":{"begin":2156,"end":2165},"obj":"VBP"},{"id":"T26970","span":{"begin":2148,"end":2155},"obj":"NNS"},{"id":"T26969","span":{"begin":2144,"end":2147},"obj":"DT"},{"id":"T26968","span":{"begin":2142,"end":2143},"obj":"."},{"id":"T26967","span":{"begin":2141,"end":2142},"obj":"NN"},{"id":"T26966","span":{"begin":2140,"end":2141},"obj":":"},{"id":"T26965","span":{"begin":2135,"end":2140},"obj":"NNP"},{"id":"T26964","span":{"begin":2132,"end":2134},"obj":"IN"},{"id":"T26963","span":{"begin":2123,"end":2131},"obj":"NNS"},{"id":"T26962","span":{"begin":2119,"end":2122},"obj":"NN"},{"id":"T26961","span":{"begin":2116,"end":2118},"obj":"CD"},{"id":"T26960","span":{"begin":2112,"end":2115},"obj":"CC"},{"id":"T26959","span":{"begin":2108,"end":2111},"obj":"NN"},{"id":"T26958","span":{"begin":2105,"end":2107},"obj":"CD"},{"id":"T26957","span":{"begin":2101,"end":2104},"obj":"DT"},{"id":"T26956","span":{"begin":2096,"end":2100},"obj":"DT"},{"id":"T26955","span":{"begin":2088,"end":2095},"obj":"VBP"},{"id":"T26954","span":{"begin":2083,"end":2087},"obj":"NNS"},{"id":"T26953","span":{"begin":2071,"end":2082},"obj":"VBN"},{"id":"T26952","span":{"begin":2070,"end":2071},"obj":":"},{"id":"T26951","span":{"begin":2063,"end":2069},"obj":"SYM"},{"id":"T26950","span":{"begin":2062,"end":2063},"obj":":"},{"id":"T26949","span":{"begin":2057,"end":2062},"obj":"NNP"},{"id":"T26948","span":{"begin":2053,"end":2056},"obj":"DT"},{"id":"T26947","span":{"begin":2051,"end":2052},"obj":"."},{"id":"T26946","span":{"begin":2044,"end":2051},"obj":"NNS"},{"id":"T26945","span":{"begin":2027,"end":2043},"obj":"NN"},{"id":"T26944","span":{"begin":2025,"end":2026},"obj":"NN"},{"id":"T26943","span":{"begin":2024,"end":2025},"obj":":"},{"id":"T26942","span":{"begin":2019,"end":2024},"obj":"NNP"},{"id":"T26941","span":{"begin":2008,"end":2018},"obj":"JJ"},{"id":"T26940","span":{"begin":2004,"end":2007},"obj":"DT"},{"id":"T26939","span":{"begin":2001,"end":2003},"obj":"IN"},{"id":"T26938","span":{"begin":1990,"end":2000},"obj":"NN"},{"id":"T26937","span":{"begin":1986,"end":1989},"obj":"DT"},{"id":"T26936","span":{"begin":1977,"end":1985},"obj":"VBZ"},{"id":"T26935","span":{"begin":1975,"end":1976},"obj":"-RRB-"},{"id":"T26934","span":{"begin":1969,"end":1975},"obj":"NNS"},{"id":"T26933","span":{"begin":1965,"end":1968},"obj":"DT"},{"id":"T26932","span":{"begin":1959,"end":1964},"obj":"IN"},{"id":"T26931","span":{"begin":1958,"end":1959},"obj":"-LRB-"},{"id":"T26930","span":{"begin":1953,"end":1957},"obj":"NN"},{"id":"T26929","span":{"begin":1945,"end":1952},"obj":"JJ"},{"id":"T26928","span":{"begin":1941,"end":1944},"obj":"DT"},{"id":"T26927","span":{"begin":1939,"end":1940},"obj":"."},{"id":"T26926","span":{"begin":1931,"end":1939},"obj":"NN"},{"id":"T26925","span":{"begin":1915,"end":1930},"obj":"JJ"},{"id":"T26924","span":{"begin":1912,"end":1914},"obj":"TO"},{"id":"T26923","span":{"begin":1901,"end":1911},"obj":"VBN"},{"id":"T26922","span":{"begin":1897,"end":1900},"obj":"CC"},{"id":"T26921","span":{"begin":1886,"end":1896},"obj":"VBN"},{"id":"T26920","span":{"begin":1882,"end":1885},"obj":"VBD"},{"id":"T26919","span":{"begin":1873,"end":1881},"obj":"NN"},{"id":"T26918","span":{"begin":1862,"end":1872},"obj":"JJ"},{"id":"T26917","span":{"begin":1860,"end":1861},"obj":"."},{"id":"T26916","span":{"begin":1857,"end":1860},"obj":"NNS"},{"id":"T26915","span":{"begin":1855,"end":1856},"obj":"CD"},{"id":"T26914","span":{"begin":1851,"end":1854},"obj":"IN"},{"id":"T26913","span":{"begin":1847,"end":1850},"obj":"NNP"},{"id":"T26912","span":{"begin":1842,"end":1846},"obj":"IN"},{"id":"T26911","span":{"begin":1831,"end":1841},"obj":"VBN"},{"id":"T26910","span":{"begin":1827,"end":1830},"obj":"CC"},{"id":"T26909","span":{"begin":1825,"end":1826},"obj":","},{"id":"T26908","span":{"begin":1817,"end":1825},"obj":"NNS"},{"id":"T26907","span":{"begin":1808,"end":1816},"obj":"NN"},{"id":"T26906","span":{"begin":1792,"end":1807},"obj":"JJ"},{"id":"T26905","span":{"begin":1788,"end":1791},"obj":"CC"},{"id":"T26904","span":{"begin":1779,"end":1787},"obj":"NN"},{"id":"T26903","span":{"begin":1759,"end":1778},"obj":"JJ"},{"id":"T26902","span":{"begin":1750,"end":1758},"obj":"NNP"},{"id":"T26901","span":{"begin":1740,"end":1749},"obj":"NNP"},{"id":"T26900","span":{"begin":1736,"end":1739},"obj":"DT"},{"id":"T26899","span":{"begin":1731,"end":1735},"obj":"IN"},{"id":"T26898","span":{"begin":1725,"end":1730},"obj":"IN"},{"id":"T26897","span":{"begin":1723,"end":1724},"obj":","},{"id":"T26896","span":{"begin":1716,"end":1723},"obj":"NNS"},{"id":"T26895","span":{"begin":1699,"end":1715},"obj":"NN"},{"id":"T26894","span":{"begin":1697,"end":1698},"obj":"NN"},{"id":"T26893","span":{"begin":1696,"end":1697},"obj":":"},{"id":"T26892","span":{"begin":1691,"end":1696},"obj":"NNP"},{"id":"T26891","span":{"begin":1681,"end":1690},"obj":"VBN"},{"id":"T26890","span":{"begin":1677,"end":1680},"obj":"DT"},{"id":"T26889","span":{"begin":1674,"end":1676},"obj":"CC"},{"id":"T26888","span":{"begin":1672,"end":1673},"obj":"NN"},{"id":"T26887","span":{"begin":1671,"end":1672},"obj":":"},{"id":"T26886","span":{"begin":1666,"end":1671},"obj":"NNP"},{"id":"T26885","span":{"begin":1656,"end":1665},"obj":"JJ"},{"id":"T26884","span":{"begin":1647,"end":1655},"obj":"VBG"},{"id":"T26883","span":{"begin":1638,"end":1646},"obj":"NNS"},{"id":"T26882","span":{"begin":1627,"end":1637},"obj":"NN"},{"id":"T26881","span":{"begin":1622,"end":1626},"obj":"IN"},{"id":"T26880","span":{"begin":1607,"end":1621},"obj":"VBN"},{"id":"T26879","span":{"begin":1602,"end":1606},"obj":"VBD"},{"id":"T26878","span":{"begin":1597,"end":1601},"obj":"NNS"},{"id":"T26877","span":{"begin":1595,"end":1596},"obj":"CD"},{"id":"T26876","span":{"begin":1594,"end":1595},"obj":"VBD"},{"id":"T26875","span":{"begin":1593,"end":1594},"obj":"NNP"},{"id":"T26874","span":{"begin":1588,"end":1593},"obj":"NNP"},{"id":"T26873","span":{"begin":1586,"end":1587},"obj":"."},{"id":"T26872","span":{"begin":1578,"end":1586},"obj":"NN"},{"id":"T26871","span":{"begin":1569,"end":1577},"obj":"NN"},{"id":"T26870","span":{"begin":1560,"end":1568},"obj":"CD"},{"id":"T26869","span":{"begin":1545,"end":1559},"obj":"JJ"},{"id":"T26868","span":{"begin":1535,"end":1544},"obj":"VBZ"},{"id":"T26867","span":{"begin":1524,"end":1534},"obj":"RB"},{"id":"T26866","span":{"begin":1521,"end":1523},"obj":"CD"},{"id":"T26865","span":{"begin":1514,"end":1520},"obj":"NNP"},{"id":"T26864","span":{"begin":1511,"end":1513},"obj":"IN"},{"id":"T26863","span":{"begin":1509,"end":1510},"obj":"NN"},{"id":"T26862","span":{"begin":1508,"end":1509},"obj":":"},{"id":"T26861","span":{"begin":1503,"end":1508},"obj":"NNP"},{"id":"T26860","span":{"begin":1500,"end":1502},"obj":"IN"},{"id":"T26859","span":{"begin":1484,"end":1499},"obj":"NNP"},{"id":"T26858","span":{"begin":1482,"end":1483},"obj":"-RRB-"},{"id":"T26857","span":{"begin":1481,"end":1482},"obj":"NNP"},{"id":"T26856","span":{"begin":1480,"end":1481},"obj":"-LRB-"},{"id":"T26855","span":{"begin":1478,"end":1479},"obj":"."},{"id":"T26854","span":{"begin":1473,"end":1478},"obj":"NNS"},{"id":"T26853","span":{"begin":1456,"end":1472},"obj":"NN"},{"id":"T26852","span":{"begin":1452,"end":1455},"obj":"CC"},{"id":"T26851","span":{"begin":1442,"end":1451},"obj":"NN"},{"id":"T26850","span":{"begin":1437,"end":1441},"obj":"NN"},{"id":"T26849","span":{"begin":1427,"end":1436},"obj":"VBG"},{"id":"T26848","span":{"begin":1417,"end":1426},"obj":"JJ"},{"id":"T26847","span":{"begin":1412,"end":1416},"obj":"NN"},{"id":"T26846","span":{"begin":1410,"end":1411},"obj":"NN"},{"id":"T26845","span":{"begin":1409,"end":1410},"obj":":"},{"id":"T26844","span":{"begin":1404,"end":1409},"obj":"SYM"},{"id":"T26843","span":{"begin":1402,"end":1403},"obj":"-RRB-"},{"id":"T26842","span":{"begin":1401,"end":1402},"obj":"NNP"},{"id":"T26841","span":{"begin":1400,"end":1401},"obj":"-LRB-"},{"id":"T26840","span":{"begin":1398,"end":1399},"obj":"."},{"id":"T26839","span":{"begin":1390,"end":1398},"obj":"NN"},{"id":"T26838","span":{"begin":1374,"end":1389},"obj":"JJ"},{"id":"T26837","span":{"begin":1371,"end":1373},"obj":"TO"},{"id":"T26836","span":{"begin":1360,"end":1370},"obj":"VBD"},{"id":"T26835","span":{"begin":1354,"end":1359},"obj":"NNS"},{"id":"T26834","span":{"begin":1343,"end":1353},"obj":"NN"},{"id":"T26833","span":{"begin":1339,"end":1342},"obj":"CC"},{"id":"T26832","span":{"begin":1337,"end":1338},"obj":","},{"id":"T26831","span":{"begin":1329,"end":1337},"obj":"NNS"},{"id":"T26830","span":{"begin":1320,"end":1328},"obj":"NN"},{"id":"T26829","span":{"begin":1304,"end":1319},"obj":"JJ"},{"id":"T26828","span":{"begin":1300,"end":1303},"obj":"CC"},{"id":"T26827","span":{"begin":1291,"end":1299},"obj":"NN"},{"id":"T26826","span":{"begin":1271,"end":1290},"obj":"JJ"},{"id":"T26825","span":{"begin":1262,"end":1270},"obj":"NNP"},{"id":"T26824","span":{"begin":1252,"end":1261},"obj":"NNP"},{"id":"T26823","span":{"begin":1248,"end":1251},"obj":"DT"},{"id":"T26822","span":{"begin":1243,"end":1247},"obj":"IN"},{"id":"T26821","span":{"begin":1228,"end":1242},"obj":"VBN"},{"id":"T26820","span":{"begin":1223,"end":1227},"obj":"VBD"},{"id":"T26819","span":{"begin":1217,"end":1222},"obj":"NNS"},{"id":"T26818","span":{"begin":1215,"end":1216},"obj":"."},{"id":"T26817","span":{"begin":1214,"end":1215},"obj":"-RRB-"},{"id":"T26816","span":{"begin":1209,"end":1214},"obj":"NNP"},{"id":"T26815","span":{"begin":1205,"end":1208},"obj":"CD"},{"id":"T26814","span":{"begin":1204,"end":1205},"obj":"-LRB-"},{"id":"T26813","span":{"begin":1200,"end":1203},"obj":"NNP"},{"id":"T26812","span":{"begin":1197,"end":1199},"obj":"IN"},{"id":"T26811","span":{"begin":1189,"end":1196},"obj":"NN"},{"id":"T26810","span":{"begin":1186,"end":1188},"obj":"CC"},{"id":"T26809","span":{"begin":1177,"end":1185},"obj":"NN"},{"id":"T26808","span":{"begin":1173,"end":1176},"obj":"DT"},{"id":"T26807","span":{"begin":1170,"end":1172},"obj":"IN"},{"id":"T26806","span":{"begin":1161,"end":1169},"obj":"NN"},{"id":"T26805","span":{"begin":1141,"end":1160},"obj":"JJ"},{"id":"T26804","span":{"begin":1132,"end":1140},"obj":"NNP"},{"id":"T26803","span":{"begin":1122,"end":1131},"obj":"NNP"},{"id":"T26802","span":{"begin":1118,"end":1121},"obj":"DT"},{"id":"T26801","span":{"begin":1107,"end":1117},"obj":"VBG"},{"id":"T26800","span":{"begin":1102,"end":1106},"obj":"NNS"},{"id":"T26799","span":{"begin":1100,"end":1101},"obj":"CD"},{"id":"T26798","span":{"begin":1099,"end":1100},"obj":"CD"},{"id":"T26797","span":{"begin":1098,"end":1099},"obj":"VBP"},{"id":"T26796","span":{"begin":1093,"end":1098},"obj":"NNP"},{"id":"T26795","span":{"begin":1089,"end":1092},"obj":"CC"},{"id":"T26794","span":{"begin":1087,"end":1088},"obj":"CD"},{"id":"T26793","span":{"begin":1086,"end":1087},"obj":"VBD"},{"id":"T26792","span":{"begin":1085,"end":1086},"obj":"NNP"},{"id":"T26791","span":{"begin":1080,"end":1085},"obj":"NNP"},{"id":"T26790","span":{"begin":1077,"end":1079},"obj":"IN"},{"id":"T26789","span":{"begin":1068,"end":1076},"obj":"VBN"},{"id":"T26788","span":{"begin":1059,"end":1067},"obj":"NN"},{"id":"T26787","span":{"begin":1050,"end":1058},"obj":"NN"},{"id":"T26786","span":{"begin":1045,"end":1049},"obj":"NN"},{"id":"T26785","span":{"begin":1036,"end":1044},"obj":"NNP"},{"id":"T26750","span":{"begin":855,"end":857},"obj":"VBZ"},{"id":"T26749","span":{"begin":853,"end":854},"obj":"NN"},{"id":"T26748","span":{"begin":852,"end":853},"obj":":"},{"id":"T26747","span":{"begin":847,"end":852},"obj":"SYM"},{"id":"T26784","span":{"begin":1029,"end":1035},"obj":"NNP"},{"id":"T26783","span":{"begin":1027,"end":1028},"obj":"."},{"id":"T26782","span":{"begin":1023,"end":1027},"obj":"NNS"},{"id":"T26781","span":{"begin":1021,"end":1022},"obj":"CD"},{"id":"T26780","span":{"begin":1020,"end":1021},"obj":"VBD"},{"id":"T26779","span":{"begin":1019,"end":1020},"obj":"NNP"},{"id":"T26778","span":{"begin":1014,"end":1019},"obj":"NNP"},{"id":"T26777","span":{"begin":1011,"end":1013},"obj":"IN"},{"id":"T26776","span":{"begin":1007,"end":1010},"obj":"RB"},{"id":"T26775","span":{"begin":1003,"end":1006},"obj":"CC"},{"id":"T26774","span":{"begin":998,"end":1002},"obj":"NNS"},{"id":"T26773","span":{"begin":996,"end":997},"obj":"CD"},{"id":"T26772","span":{"begin":995,"end":996},"obj":"VBD"},{"id":"T26771","span":{"begin":994,"end":995},"obj":"NNP"},{"id":"T26770","span":{"begin":989,"end":994},"obj":"NNP"},{"id":"T26769","span":{"begin":974,"end":988},"obj":"JJ"},{"id":"T26768","span":{"begin":971,"end":973},"obj":"IN"},{"id":"T26767","span":{"begin":961,"end":970},"obj":"VBN"},{"id":"T26766","span":{"begin":947,"end":960},"obj":"RB"},{"id":"T26765","span":{"begin":944,"end":946},"obj":"VBZ"},{"id":"T26764","span":{"begin":935,"end":943},"obj":"NN"},{"id":"T26763","span":{"begin":926,"end":934},"obj":"JJ"},{"id":"T26762","span":{"begin":917,"end":925},"obj":"JJ"},{"id":"T26761","span":{"begin":913,"end":916},"obj":"DT"},{"id":"T26760","span":{"begin":911,"end":912},"obj":"-RRB-"},{"id":"T26759","span":{"begin":910,"end":911},"obj":"NNP"},{"id":"T26758","span":{"begin":909,"end":910},"obj":"-LRB-"},{"id":"T26757","span":{"begin":907,"end":908},"obj":"."},{"id":"T26756","span":{"begin":899,"end":907},"obj":"NN"},{"id":"T26755","span":{"begin":890,"end":898},"obj":"NN"},{"id":"T26754","span":{"begin":881,"end":889},"obj":"JJ"},{"id":"T26753","span":{"begin":872,"end":880},"obj":"NNP"},{"id":"T26752","span":{"begin":868,"end":871},"obj":"IN"},{"id":"T26751","span":{"begin":858,"end":867},"obj":"JJ"},{"id":"T16214","span":{"begin":634,"end":643},"obj":"VBN"},{"id":"T16213","span":{"begin":620,"end":633},"obj":"RB"},{"id":"T16212","span":{"begin":616,"end":619},"obj":"VBD"},{"id":"T16211","span":{"begin":607,"end":615},"obj":"NN"},{"id":"T16210","span":{"begin":598,"end":606},"obj":"NN"},{"id":"T16209","span":{"begin":589,"end":597},"obj":"JJ"},{"id":"T16208","span":{"begin":574,"end":588},"obj":"JJ"},{"id":"T16207","span":{"begin":569,"end":573},"obj":"IN"},{"id":"T16206","span":{"begin":563,"end":568},"obj":"VBD"},{"id":"T16205","span":{"begin":560,"end":562},"obj":"PRP"},{"id":"T16204","span":{"begin":558,"end":559},"obj":"."},{"id":"T16203","span":{"begin":554,"end":558},"obj":"NNS"},{"id":"T16202","span":{"begin":552,"end":553},"obj":"CD"},{"id":"T16201","span":{"begin":551,"end":552},"obj":"CD"},{"id":"T16200","span":{"begin":550,"end":551},"obj":"VBP"},{"id":"T16199","span":{"begin":545,"end":550},"obj":"NNP"},{"id":"T16198","span":{"begin":541,"end":544},"obj":"CC"},{"id":"T16197","span":{"begin":539,"end":540},"obj":"CD"},{"id":"T16196","span":{"begin":538,"end":539},"obj":"VBD"},{"id":"T16195","span":{"begin":537,"end":538},"obj":"NNP"},{"id":"T16194","span":{"begin":532,"end":537},"obj":"NNP"},{"id":"T16193","span":{"begin":527,"end":531},"obj":"IN"},{"id":"T16192","span":{"begin":517,"end":526},"obj":"VBP"},{"id":"T16191","span":{"begin":508,"end":516},"obj":"NN"},{"id":"T16190","span":{"begin":497,"end":507},"obj":"NN"},{"id":"T16189","span":{"begin":488,"end":496},"obj":"NNP"},{"id":"T16188","span":{"begin":481,"end":487},"obj":"NN"},{"id":"T16187","span":{"begin":471,"end":480},"obj":"JJ"},{"id":"T16186","span":{"begin":467,"end":470},"obj":"DT"},{"id":"T16185","span":{"begin":464,"end":466},"obj":"IN"},{"id":"T16184","span":{"begin":451,"end":463},"obj":"NN"},{"id":"T16183","span":{"begin":448,"end":450},"obj":"IN"},{"id":"T16182","span":{"begin":439,"end":447},"obj":"VBN"},{"id":"T16181","span":{"begin":435,"end":438},"obj":"VBD"},{"id":"T16180","span":{"begin":426,"end":434},"obj":"NN"},{"id":"T16179","span":{"begin":417,"end":425},"obj":"JJ"},{"id":"T16178","span":{"begin":408,"end":416},"obj":"NNP"},{"id":"T16177","span":{"begin":404,"end":407},"obj":"DT"},{"id":"T16176","span":{"begin":399,"end":403},"obj":"IN"},{"id":"T16175","span":{"begin":385,"end":398},"obj":"NN"},{"id":"T16174","span":{"begin":378,"end":384},"obj":"VB"},{"id":"T16173","span":{"begin":375,"end":377},"obj":"TO"},{"id":"T16172","span":{"begin":373,"end":374},"obj":"NN"},{"id":"T16171","span":{"begin":372,"end":373},"obj":":"},{"id":"T16170","span":{"begin":367,"end":372},"obj":"NNP"},{"id":"T16169","span":{"begin":356,"end":366},"obj":"JJ"},{"id":"T16168","span":{"begin":353,"end":355},"obj":"IN"},{"id":"T16167","span":{"begin":345,"end":352},"obj":"NN"},{"id":"T16166","span":{"begin":341,"end":344},"obj":"DT"},{"id":"T16165","span":{"begin":339,"end":340},"obj":"."},{"id":"T16164","span":{"begin":336,"end":339},"obj":"NNP"},{"id":"T16163","span":{"begin":333,"end":335},"obj":"TO"},{"id":"T16162","span":{"begin":324,"end":332},"obj":"NN"},{"id":"T16161","span":{"begin":321,"end":323},"obj":"IN"},{"id":"T16160","span":{"begin":315,"end":320},"obj":"NNS"},{"id":"T16159","span":{"begin":312,"end":314},"obj":"IN"},{"id":"T16158","span":{"begin":303,"end":311},"obj":"NN"},{"id":"T16157","span":{"begin":294,"end":302},"obj":"NNP"},{"id":"T16156","span":{"begin":290,"end":293},"obj":"DT"},{"id":"T16155","span":{"begin":285,"end":289},"obj":"IN"},{"id":"T16154","span":{"begin":271,"end":284},"obj":"NN"},{"id":"T16153","span":{"begin":263,"end":270},"obj":"VBG"},{"id":"T16152","span":{"begin":259,"end":262},"obj":"IN"},{"id":"T16151","span":{"begin":252,"end":258},"obj":"NN"},{"id":"T16150","span":{"begin":242,"end":251},"obj":"JJ"},{"id":"T16149","span":{"begin":239,"end":241},"obj":"DT"},{"id":"T16148","span":{"begin":235,"end":238},"obj":"VBD"},{"id":"T16147","span":{"begin":233,"end":234},"obj":"NN"},{"id":"T16146","span":{"begin":232,"end":233},"obj":":"},{"id":"T16145","span":{"begin":227,"end":232},"obj":"NNP"},{"id":"T16144","span":{"begin":219,"end":226},"obj":"IN"},{"id":"T16143","span":{"begin":216,"end":218},"obj":"IN"},{"id":"T16142","span":{"begin":207,"end":215},"obj":"NN"},{"id":"T16141","span":{"begin":203,"end":206},"obj":"DT"},{"id":"T16140","span":{"begin":195,"end":202},"obj":"VB"},{"id":"T16139","span":{"begin":192,"end":194},"obj":"TO"},{"id":"T16138","span":{"begin":185,"end":191},"obj":"VBD"},{"id":"T16137","span":{"begin":182,"end":184},"obj":"PRP"},{"id":"T16136","span":{"begin":180,"end":181},"obj":","},{"id":"T16135","span":{"begin":176,"end":180},"obj":"NN"},{"id":"T16134","span":{"begin":173,"end":175},"obj":"IN"},{"id":"T16133","span":{"begin":169,"end":172},"obj":"CC"},{"id":"T16132","span":{"begin":163,"end":168},"obj":"NN"},{"id":"T16131","span":{"begin":160,"end":162},"obj":"IN"},{"id":"T16130","span":{"begin":155,"end":159},"obj":"DT"},{"id":"T16129","span":{"begin":148,"end":154},"obj":"NN"},{"id":"T16128","span":{"begin":134,"end":147},"obj":"JJ"},{"id":"T16127","span":{"begin":131,"end":133},"obj":"DT"},{"id":"T16126","span":{"begin":128,"end":130},"obj":"IN"},{"id":"T16125","span":{"begin":119,"end":127},"obj":"NN"},{"id":"T16124","span":{"begin":110,"end":118},"obj":"JJ"},{"id":"T16123","span":{"begin":101,"end":109},"obj":"NNP"},{"id":"T16122","span":{"begin":97,"end":100},"obj":"DT"},{"id":"T16121","span":{"begin":94,"end":96},"obj":"TO"},{"id":"T16120","span":{"begin":88,"end":93},"obj":"NNS"},{"id":"T16119","span":{"begin":86,"end":87},"obj":"NN"},{"id":"T16118","span":{"begin":85,"end":86},"obj":":"},{"id":"T16117","span":{"begin":80,"end":85},"obj":"NNP"},{"id":"T16116","span":{"begin":74,"end":79},"obj":"IN"},{"id":"T16115","span":{"begin":65,"end":73},"obj":"NNP"},{"id":"T16114","span":{"begin":56,"end":64},"obj":"NNP"},{"id":"T16113","span":{"begin":47,"end":55},"obj":"NNP"},{"id":"T16112","span":{"begin":38,"end":46},"obj":"NNP"},{"id":"T16111","span":{"begin":31,"end":37},"obj":"NNP"},{"id":"T16110","span":{"begin":17,"end":30},"obj":"JJ"},{"id":"T16109","span":{"begin":8,"end":16},"obj":"VBZ"},{"id":"T16108","span":{"begin":6,"end":7},"obj":"NN"},{"id":"T16107","span":{"begin":5,"end":6},"obj":":"},{"id":"T16248","span":{"begin":801,"end":802},"obj":"."},{"id":"T16247","span":{"begin":793,"end":801},"obj":"NN"},{"id":"T16246","span":{"begin":784,"end":792},"obj":"NN"},{"id":"T16245","span":{"begin":775,"end":783},"obj":"JJ"},{"id":"T16244","span":{"begin":766,"end":774},"obj":"JJ"},{"id":"T16243","span":{"begin":754,"end":765},"obj":"JJ"},{"id":"T16242","span":{"begin":750,"end":753},"obj":"IN"},{"id":"T16241","span":{"begin":741,"end":749},"obj":"VBN"},{"id":"T16240","span":{"begin":738,"end":740},"obj":"VBZ"},{"id":"T16239","span":{"begin":736,"end":737},"obj":"NN"},{"id":"T16238","span":{"begin":735,"end":736},"obj":":"},{"id":"T16237","span":{"begin":730,"end":735},"obj":"NNP"},{"id":"T16236","span":{"begin":719,"end":729},"obj":"JJ"},{"id":"T16235","span":{"begin":714,"end":718},"obj":"IN"},{"id":"T16234","span":{"begin":703,"end":713},"obj":"VBG"},{"id":"T16233","span":{"begin":701,"end":702},"obj":","},{"id":"T16232","span":{"begin":700,"end":701},"obj":"-RRB-"},{"id":"T16231","span":{"begin":698,"end":700},"obj":"NNP"},{"id":"T16230","span":{"begin":693,"end":697},"obj":"NNP"},{"id":"T16229","span":{"begin":692,"end":693},"obj":"-LRB-"},{"id":"T16228","span":{"begin":687,"end":691},"obj":"NNS"},{"id":"T16227","span":{"begin":685,"end":686},"obj":"CD"},{"id":"T16226","span":{"begin":684,"end":685},"obj":"VBD"},{"id":"T16225","span":{"begin":683,"end":684},"obj":"NNP"},{"id":"T16224","span":{"begin":678,"end":683},"obj":"NNP"},{"id":"T16223","span":{"begin":675,"end":677},"obj":"IN"},{"id":"T16222","span":{"begin":665,"end":674},"obj":"VBD"},{"id":"T16221","span":{"begin":661,"end":664},"obj":"CC"},{"id":"T16220","span":{"begin":656,"end":660},"obj":"NNS"},{"id":"T16219","span":{"begin":654,"end":655},"obj":"CD"},{"id":"T16218","span":{"begin":653,"end":654},"obj":"VBD"},{"id":"T16217","span":{"begin":652,"end":653},"obj":"NNP"},{"id":"T16216","span":{"begin":647,"end":652},"obj":"NNP"},{"id":"T16215","span":{"begin":644,"end":646},"obj":"IN"},{"id":"T16106","span":{"begin":0,"end":5},"obj":"SYM"}],"relations":[{"id":"R11807","pred":"ROOT","subj":"T16106","obj":"T16106"},{"id":"R11808","pred":"punct","subj":"T16107","obj":"T16109"},{"id":"R11809","pred":"nsubj","subj":"T16108","obj":"T16109"},{"id":"R11810","pred":"ROOT","subj":"T16109","obj":"T16109"},{"id":"R11811","pred":"amod","subj":"T16110","obj":"T16115"},{"id":"R11812","pred":"compound","subj":"T16111","obj":"T16113"},{"id":"R11813","pred":"compound","subj":"T16112","obj":"T16113"},{"id":"R11814","pred":"compound","subj":"T16113","obj":"T16115"},{"id":"R11815","pred":"compound","subj":"T16114","obj":"T16115"},{"id":"R11816","pred":"dobj","subj":"T16115","obj":"T16109"},{"id":"R11817","pred":"prep","subj":"T16116","obj":"T16115"},{"id":"R11818","pred":"compound","subj":"T16117","obj":"T16119"},{"id":"R11819","pred":"punct","subj":"T16118","obj":"T16119"},{"id":"R11820","pred":"compound","subj":"T16119","obj":"T16120"},{"id":"R11821","pred":"pobj","subj":"T16120","obj":"T16116"},{"id":"R11822","pred":"prep","subj":"T16121","obj":"T16109"},{"id":"R11823","pred":"det","subj":"T16122","obj":"T16125"},{"id":"R11824","pred":"nmod","subj":"T16123","obj":"T16125"},{"id":"R11825","pred":"amod","subj":"T16124","obj":"T16125"},{"id":"R11826","pred":"pobj","subj":"T16125","obj":"T16121"},{"id":"R11827","pred":"prep","subj":"T16126","obj":"T16125"},{"id":"R11828","pred":"det","subj":"T16127","obj":"T16129"},{"id":"R11829","pred":"amod","subj":"T16128","obj":"T16129"},{"id":"R11830","pred":"pobj","subj":"T16129","obj":"T16126"},{"id":"R11831","pred":"preconj","subj":"T16130","obj":"T16131"},{"id":"R11832","pred":"prep","subj":"T16131","obj":"T16109"},{"id":"R11833","pred":"pobj","subj":"T16132","obj":"T16131"},{"id":"R11834","pred":"cc","subj":"T16133","obj":"T16131"},{"id":"R11835","pred":"conj","subj":"T16134","obj":"T16131"},{"id":"R11836","pred":"pobj","subj":"T16135","obj":"T16134"},{"id":"R11837","pred":"punct","subj":"T16136","obj":"T16138"},{"id":"R11838","pred":"nsubj","subj":"T16137","obj":"T16138"},{"id":"R11839","pred":"ROOT","subj":"T16138","obj":"T16138"},{"id":"R11840","pred":"aux","subj":"T16139","obj":"T16140"},{"id":"R11841","pred":"xcomp","subj":"T16140","obj":"T16138"},{"id":"R11842","pred":"det","subj":"T16141","obj":"T16142"},{"id":"R11843","pred":"dobj","subj":"T16142","obj":"T16140"},{"id":"R11844","pred":"prep","subj":"T16143","obj":"T16142"},{"id":"R11845","pred":"mark","subj":"T16144","obj":"T16148"},{"id":"R11846","pred":"compound","subj":"T16145","obj":"T16147"},{"id":"R11847","pred":"punct","subj":"T16146","obj":"T16147"},{"id":"R11848","pred":"nsubj","subj":"T16147","obj":"T16148"},{"id":"R11849","pred":"pcomp","subj":"T16148","obj":"T16143"},{"id":"R11850","pred":"det","subj":"T16149","obj":"T16151"},{"id":"R11851","pred":"amod","subj":"T16150","obj":"T16151"},{"id":"R11852","pred":"attr","subj":"T16151","obj":"T16148"},{"id":"R11853","pred":"prep","subj":"T16152","obj":"T16151"},{"id":"R11854","pred":"pcomp","subj":"T16153","obj":"T16152"},{"id":"R11855","pred":"dobj","subj":"T16154","obj":"T16153"},{"id":"R11856","pred":"prep","subj":"T16155","obj":"T16153"},{"id":"R11857","pred":"det","subj":"T16156","obj":"T16158"},{"id":"R11858","pred":"compound","subj":"T16157","obj":"T16158"},{"id":"R11859","pred":"pobj","subj":"T16158","obj":"T16155"},{"id":"R11860","pred":"prep","subj":"T16159","obj":"T16158"},{"id":"R11861","pred":"pobj","subj":"T16160","obj":"T16159"},{"id":"R11862","pred":"prep","subj":"T16161","obj":"T16153"},{"id":"R11863","pred":"pobj","subj":"T16162","obj":"T16161"},{"id":"R11864","pred":"prep","subj":"T16163","obj":"T16162"},{"id":"R11865","pred":"pobj","subj":"T16164","obj":"T16163"},{"id":"R11866","pred":"punct","subj":"T16165","obj":"T16138"},{"id":"R11867","pred":"det","subj":"T16166","obj":"T16167"},{"id":"R11868","pred":"nsubjpass","subj":"T16167","obj":"T16182"},{"id":"R11869","pred":"prep","subj":"T16168","obj":"T16167"},{"id":"R11870","pred":"amod","subj":"T16169","obj":"T16172"},{"id":"R11871","pred":"compound","subj":"T16170","obj":"T16172"},{"id":"R11872","pred":"punct","subj":"T16171","obj":"T16172"},{"id":"R11873","pred":"pobj","subj":"T16172","obj":"T16168"},{"id":"R11874","pred":"aux","subj":"T16173","obj":"T16174"},{"id":"R11875","pred":"acl","subj":"T16174","obj":"T16167"},{"id":"R11876","pred":"dobj","subj":"T16175","obj":"T16174"},{"id":"R11877","pred":"prep","subj":"T16176","obj":"T16174"},{"id":"R11878","pred":"det","subj":"T16177","obj":"T16180"},{"id":"R11879","pred":"nmod","subj":"T16178","obj":"T16180"},{"id":"R11880","pred":"amod","subj":"T16179","obj":"T16180"},{"id":"R11881","pred":"pobj","subj":"T16180","obj":"T16176"},{"id":"R11882","pred":"auxpass","subj":"T16181","obj":"T16182"},{"id":"R11883","pred":"ROOT","subj":"T16182","obj":"T16182"},{"id":"R11884","pred":"agent","subj":"T16183","obj":"T16182"},{"id":"R11885","pred":"pobj","subj":"T16184","obj":"T16183"},{"id":"R11886","pred":"prep","subj":"T16185","obj":"T16184"},{"id":"R11887","pred":"det","subj":"T16186","obj":"T16191"},{"id":"R11888","pred":"amod","subj":"T16187","obj":"T16191"},{"id":"R11889","pred":"compound","subj":"T16188","obj":"T16191"},{"id":"R11890","pred":"compound","subj":"T16189","obj":"T16191"},{"id":"R11891","pred":"compound","subj":"T16190","obj":"T16191"},{"id":"R11892","pred":"pobj","subj":"T16191","obj":"T16185"},{"id":"R11893","pred":"xcomp","subj":"T16192","obj":"T16182"},{"id":"R11894","pred":"prep","subj":"T16193","obj":"T16192"},{"id":"R11895","pred":"compound","subj":"T16194","obj":"T16195"},{"id":"R11896","pred":"pobj","subj":"T16195","obj":"T16193"},{"id":"R11897","pred":"conj","subj":"T16196","obj":"T16182"},{"id":"R11898","pred":"dobj","subj":"T16197","obj":"T16196"},{"id":"R11899","pred":"cc","subj":"T16198","obj":"T16196"},{"id":"R11900","pred":"nsubj","subj":"T16199","obj":"T16200"},{"id":"R11901","pred":"conj","subj":"T16200","obj":"T16196"},{"id":"R11902","pred":"nummod","subj":"T16201","obj":"T16203"},{"id":"R11903","pred":"nummod","subj":"T16202","obj":"T16203"},{"id":"R11904","pred":"dobj","subj":"T16203","obj":"T16200"},{"id":"R11905","pred":"punct","subj":"T16204","obj":"T16200"},{"id":"R11906","pred":"nsubj","subj":"T16205","obj":"T16206"},{"id":"R11907","pred":"ROOT","subj":"T16206","obj":"T16206"},{"id":"R11908","pred":"mark","subj":"T16207","obj":"T16214"},{"id":"R11909","pred":"amod","subj":"T16208","obj":"T16211"},{"id":"R11910","pred":"amod","subj":"T16209","obj":"T16211"},{"id":"R11911","pred":"compound","subj":"T16210","obj":"T16211"},{"id":"R11912","pred":"nsubjpass","subj":"T16211","obj":"T16214"},{"id":"R11913","pred":"auxpass","subj":"T16212","obj":"T16214"},{"id":"R11914","pred":"advmod","subj":"T16213","obj":"T16214"},{"id":"R11915","pred":"ccomp","subj":"T16214","obj":"T16206"},{"id":"R11916","pred":"prep","subj":"T16215","obj":"T16214"},{"id":"R11917","pred":"compound","subj":"T16216","obj":"T16217"},{"id":"R11918","pred":"pobj","subj":"T16217","obj":"T16215"},{"id":"R11919","pred":"advcl","subj":"T16218","obj":"T16214"},{"id":"R11920","pred":"nummod","subj":"T16219","obj":"T16220"},{"id":"R11921","pred":"dobj","subj":"T16220","obj":"T16218"},{"id":"R11922","pred":"cc","subj":"T16221","obj":"T16218"},{"id":"R11923","pred":"conj","subj":"T16222","obj":"T16218"},{"id":"R11924","pred":"prep","subj":"T16223","obj":"T16222"},{"id":"R11925","pred":"compound","subj":"T16224","obj":"T16225"},{"id":"R11926","pred":"pobj","subj":"T16225","obj":"T16223"},{"id":"R11927","pred":"conj","subj":"T16226","obj":"T16218"},{"id":"R11928","pred":"nummod","subj":"T16227","obj":"T16228"},{"id":"R11929","pred":"dobj","subj":"T16228","obj":"T16226"},{"id":"R11930","pred":"punct","subj":"T16229","obj":"T16228"},{"id":"R11931","pred":"compound","subj":"T16230","obj":"T16231"},{"id":"R11932","pred":"appos","subj":"T16231","obj":"T16228"},{"id":"R11933","pred":"punct","subj":"T16232","obj":"T16228"},{"id":"R11934","pred":"punct","subj":"T16233","obj":"T16226"},{"id":"R11935","pred":"advcl","subj":"T16234","obj":"T16226"},{"id":"R11936","pred":"mark","subj":"T16235","obj":"T16241"},{"id":"R11937","pred":"amod","subj":"T16236","obj":"T16239"},{"id":"R11938","pred":"compound","subj":"T16237","obj":"T16239"},{"id":"R11939","pred":"punct","subj":"T16238","obj":"T16239"},{"id":"R11940","pred":"nsubjpass","subj":"T16239","obj":"T16241"},{"id":"R11941","pred":"auxpass","subj":"T16240","obj":"T16241"},{"id":"R11942","pred":"ccomp","subj":"T16241","obj":"T16234"},{"id":"R11943","pred":"prep","subj":"T16242","obj":"T16241"},{"id":"R11944","pred":"amod","subj":"T16243","obj":"T16247"},{"id":"R11945","pred":"amod","subj":"T16244","obj":"T16247"},{"id":"R11946","pred":"amod","subj":"T16245","obj":"T16247"},{"id":"R11947","pred":"compound","subj":"T16246","obj":"T16247"},{"id":"R11948","pred":"pobj","subj":"T16247","obj":"T16242"},{"id":"R11949","pred":"punct","subj":"T16248","obj":"T16206"},{"id":"R18885","pred":"amod","subj":"T26747","obj":"T26749"},{"id":"R18886","pred":"punct","subj":"T26748","obj":"T26749"},{"id":"R18887","pred":"nsubj","subj":"T26749","obj":"T26750"},{"id":"R18888","pred":"ROOT","subj":"T26750","obj":"T26750"},{"id":"R18889","pred":"acomp","subj":"T26751","obj":"T26750"},{"id":"R18890","pred":"prep","subj":"T26752","obj":"T26750"},{"id":"R18891","pred":"nmod","subj":"T26753","obj":"T26756"},{"id":"R18892","pred":"amod","subj":"T26754","obj":"T26756"},{"id":"R18893","pred":"compound","subj":"T26755","obj":"T26756"},{"id":"R18894","pred":"pobj","subj":"T26756","obj":"T26752"},{"id":"R18895","pred":"punct","subj":"T26757","obj":"T26750"},{"id":"R18896","pred":"punct","subj":"T26758","obj":"T26759"},{"id":"R18897","pred":"ROOT","subj":"T26759","obj":"T26759"},{"id":"R18898","pred":"punct","subj":"T26760","obj":"T26759"},{"id":"R18899","pred":"det","subj":"T26761","obj":"T26764"},{"id":"R18900","pred":"amod","subj":"T26762","obj":"T26764"},{"id":"R18901","pred":"amod","subj":"T26763","obj":"T26764"},{"id":"R18902","pred":"nsubjpass","subj":"T26764","obj":"T26767"},{"id":"R18903","pred":"auxpass","subj":"T26765","obj":"T26767"},{"id":"R18904","pred":"advmod","subj":"T26766","obj":"T26767"},{"id":"R18905","pred":"ROOT","subj":"T26767","obj":"T26767"},{"id":"R18906","pred":"prep","subj":"T26768","obj":"T26767"},{"id":"R18907","pred":"amod","subj":"T26769","obj":"T26771"},{"id":"R18908","pred":"compound","subj":"T26770","obj":"T26771"},{"id":"R18909","pred":"compound","subj":"T26771","obj":"T26780"},{"id":"R18910","pred":"dep","subj":"T26772","obj":"T26780"},{"id":"R18911","pred":"nummod","subj":"T26773","obj":"T26774"},{"id":"R18912","pred":"dobj","subj":"T26774","obj":"T26772"},{"id":"R18913","pred":"cc","subj":"T26775","obj":"T26774"},{"id":"R18914","pred":"neg","subj":"T26776","obj":"T26777"},{"id":"R18915","pred":"prep","subj":"T26777","obj":"T26772"},{"id":"R18929","pred":"compound","subj":"T26791","obj":"T26792"},{"id":"R18930","pred":"compound","subj":"T26792","obj":"T26793"},{"id":"R18931","pred":"ROOT","subj":"T26793","obj":"T26793"},{"id":"R18932","pred":"dobj","subj":"T26794","obj":"T26793"},{"id":"R18933","pred":"cc","subj":"T26795","obj":"T26793"},{"id":"R18934","pred":"nsubj","subj":"T26796","obj":"T26797"},{"id":"R18935","pred":"conj","subj":"T26797","obj":"T26793"},{"id":"R18936","pred":"nummod","subj":"T26798","obj":"T26800"},{"id":"R18937","pred":"nummod","subj":"T26799","obj":"T26800"},{"id":"R18938","pred":"dobj","subj":"T26800","obj":"T26797"},{"id":"R18939","pred":"acl","subj":"T26801","obj":"T26800"},{"id":"R18940","pred":"det","subj":"T26802","obj":"T26806"},{"id":"R18941","pred":"compound","subj":"T26803","obj":"T26804"},{"id":"R18942","pred":"nmod","subj":"T26804","obj":"T26806"},{"id":"R18943","pred":"amod","subj":"T26805","obj":"T26806"},{"id":"R18944","pred":"dobj","subj":"T26806","obj":"T26801"},{"id":"R18945","pred":"prep","subj":"T26807","obj":"T26801"},{"id":"R18946","pred":"det","subj":"T26808","obj":"T26809"},{"id":"R18947","pred":"pobj","subj":"T26809","obj":"T26807"},{"id":"R18948","pred":"cc","subj":"T26810","obj":"T26809"},{"id":"R18949","pred":"conj","subj":"T26811","obj":"T26809"},{"id":"R18950","pred":"prep","subj":"T26812","obj":"T26811"},{"id":"R18951","pred":"pobj","subj":"T26813","obj":"T26812"},{"id":"R18952","pred":"punct","subj":"T26814","obj":"T26816"},{"id":"R18953","pred":"nummod","subj":"T26815","obj":"T26816"},{"id":"R18954","pred":"appos","subj":"T26816","obj":"T26813"},{"id":"R18955","pred":"punct","subj":"T26817","obj":"T26797"},{"id":"R18956","pred":"punct","subj":"T26818","obj":"T26793"},{"id":"R18957","pred":"nsubjpass","subj":"T26819","obj":"T26821"},{"id":"R18958","pred":"auxpass","subj":"T26820","obj":"T26821"},{"id":"R18959","pred":"ROOT","subj":"T26821","obj":"T26821"},{"id":"R18960","pred":"prep","subj":"T26822","obj":"T26821"},{"id":"R18961","pred":"det","subj":"T26823","obj":"T26827"},{"id":"R18962","pred":"nmod","subj":"T26824","obj":"T26827"},{"id":"R18963","pred":"nmod","subj":"T26825","obj":"T26827"},{"id":"R18964","pred":"amod","subj":"T26826","obj":"T26827"},{"id":"R18965","pred":"pobj","subj":"T26827","obj":"T26822"},{"id":"R18966","pred":"cc","subj":"T26828","obj":"T26827"},{"id":"R18967","pred":"amod","subj":"T26829","obj":"T26830"},{"id":"R18968","pred":"conj","subj":"T26830","obj":"T26827"},{"id":"R18969","pred":"conj","subj":"T26831","obj":"T26827"},{"id":"R18970","pred":"punct","subj":"T26832","obj":"T26831"},{"id":"R18971","pred":"cc","subj":"T26833","obj":"T26831"},{"id":"R18972","pred":"compound","subj":"T26834","obj":"T26835"},{"id":"R18973","pred":"conj","subj":"T26835","obj":"T26831"},{"id":"R18974","pred":"conj","subj":"T26836","obj":"T26821"},{"id":"R18975","pred":"prep","subj":"T26837","obj":"T26836"},{"id":"R18976","pred":"amod","subj":"T26838","obj":"T26839"},{"id":"R18977","pred":"pobj","subj":"T26839","obj":"T26837"},{"id":"R18978","pred":"punct","subj":"T26840","obj":"T26821"},{"id":"R18979","pred":"punct","subj":"T26841","obj":"T26842"},{"id":"R18980","pred":"nmod","subj":"T26842","obj":"T26848"},{"id":"R18981","pred":"punct","subj":"T26843","obj":"T26842"},{"id":"R18982","pred":"punct","subj":"T26844","obj":"T26846"},{"id":"R18983","pred":"punct","subj":"T26845","obj":"T26846"},{"id":"R18984","pred":"compound","subj":"T26846","obj":"T26847"},{"id":"R18985","pred":"compound","subj":"T26847","obj":"T26848"},{"id":"R18986","pred":"ROOT","subj":"T26848","obj":"T26848"},{"id":"R18987","pred":"xcomp","subj":"T26849","obj":"T26848"},{"id":"R18988","pred":"compound","subj":"T26850","obj":"T26851"},{"id":"R18989","pred":"dobj","subj":"T26851","obj":"T26849"},{"id":"R18990","pred":"cc","subj":"T26852","obj":"T26851"},{"id":"R18991","pred":"compound","subj":"T26853","obj":"T26854"},{"id":"R18992","pred":"conj","subj":"T26854","obj":"T26851"},{"id":"R18993","pred":"punct","subj":"T26855","obj":"T26848"},{"id":"R18994","pred":"punct","subj":"T26856","obj":"T26859"},{"id":"R18995","pred":"nmod","subj":"T26857","obj":"T26859"},{"id":"R18996","pred":"punct","subj":"T26858","obj":"T26859"},{"id":"R18997","pred":"nsubj","subj":"T26859","obj":"T26868"},{"id":"R18998","pred":"prep","subj":"T26860","obj":"T26859"},{"id":"R18999","pred":"pobj","subj":"T26861","obj":"T26860"},{"id":"R19000","pred":"punct","subj":"T26862","obj":"T26863"},{"id":"R19001","pred":"appos","subj":"T26863","obj":"T26859"},{"id":"R19002","pred":"prep","subj":"T26864","obj":"T26863"},{"id":"R19003","pred":"pobj","subj":"T26865","obj":"T26864"},{"id":"R19004","pred":"nummod","subj":"T26866","obj":"T26865"},{"id":"R19005","pred":"advmod","subj":"T26867","obj":"T26868"},{"id":"R19006","pred":"ROOT","subj":"T26868","obj":"T26868"},{"id":"R19007","pred":"amod","subj":"T26869","obj":"T26872"},{"id":"R19008","pred":"nummod","subj":"T26870","obj":"T26872"},{"id":"R19009","pred":"compound","subj":"T26871","obj":"T26872"},{"id":"R19010","pred":"dobj","subj":"T26872","obj":"T26868"},{"id":"R19011","pred":"punct","subj":"T26873","obj":"T26868"},{"id":"R19012","pred":"compound","subj":"T26874","obj":"T26875"},{"id":"R19013","pred":"nsubj","subj":"T26875","obj":"T26876"},{"id":"R19014","pred":"ROOT","subj":"T26876","obj":"T26876"},{"id":"R19015","pred":"nummod","subj":"T26877","obj":"T26878"},{"id":"R19016","pred":"nsubjpass","subj":"T26878","obj":"T26880"},{"id":"R19017","pred":"auxpass","subj":"T26879","obj":"T26880"},{"id":"R19018","pred":"ccomp","subj":"T26880","obj":"T26876"},{"id":"R19019","pred":"prep","subj":"T26881","obj":"T26880"},{"id":"R19020","pred":"compound","subj":"T26882","obj":"T26883"},{"id":"R19021","pred":"nsubj","subj":"T26883","obj":"T26884"},{"id":"R19022","pred":"pcomp","subj":"T26884","obj":"T26881"},{"id":"R19023","pred":"amod","subj":"T26885","obj":"T26888"},{"id":"R19024","pred":"compound","subj":"T26886","obj":"T26888"},{"id":"R19025","pred":"punct","subj":"T26887","obj":"T26888"},{"id":"R19026","pred":"dobj","subj":"T26888","obj":"T26884"},{"id":"R19027","pred":"cc","subj":"T26889","obj":"T26888"},{"id":"R19028","pred":"det","subj":"T26890","obj":"T26896"},{"id":"R19029","pred":"amod","subj":"T26891","obj":"T26896"},{"id":"R19030","pred":"nmod","subj":"T26892","obj":"T26896"},{"id":"R19031","pred":"punct","subj":"T26893","obj":"T26894"},{"id":"R19032","pred":"nmod","subj":"T26894","obj":"T26896"},{"id":"R19033","pred":"compound","subj":"T26895","obj":"T26896"},{"id":"R19034","pred":"conj","subj":"T26896","obj":"T26888"},{"id":"R19035","pred":"punct","subj":"T26897","obj":"T26896"},{"id":"R19036","pred":"prep","subj":"T26898","obj":"T26884"},{"id":"R19037","pred":"prep","subj":"T26899","obj":"T26898"},{"id":"R19038","pred":"det","subj":"T26900","obj":"T26904"},{"id":"R19039","pred":"compound","subj":"T26901","obj":"T26902"},{"id":"R19040","pred":"nmod","subj":"T26902","obj":"T26904"},{"id":"R19041","pred":"amod","subj":"T26903","obj":"T26904"},{"id":"R19042","pred":"pobj","subj":"T26904","obj":"T26899"},{"id":"R19043","pred":"cc","subj":"T26905","obj":"T26904"},{"id":"R19044","pred":"amod","subj":"T26906","obj":"T26907"},{"id":"R19045","pred":"conj","subj":"T26907","obj":"T26904"},{"id":"R19046","pred":"conj","subj":"T26908","obj":"T26904"},{"id":"R19047","pred":"punct","subj":"T26909","obj":"T26908"},{"id":"R19048","pred":"cc","subj":"T26910","obj":"T26880"},{"id":"R19049","pred":"conj","subj":"T26911","obj":"T26880"},{"id":"R19050","pred":"prep","subj":"T26912","obj":"T26911"},{"id":"R19051","pred":"pobj","subj":"T26913","obj":"T26912"},{"id":"R19052","pred":"prep","subj":"T26914","obj":"T26911"},{"id":"R19053","pred":"nummod","subj":"T26915","obj":"T26916"},{"id":"R19054","pred":"pobj","subj":"T26916","obj":"T26914"},{"id":"R19055","pred":"punct","subj":"T26917","obj":"T26876"},{"id":"R19056","pred":"amod","subj":"T26918","obj":"T26919"},{"id":"R19057","pred":"nsubjpass","subj":"T26919","obj":"T26921"},{"id":"R19058","pred":"auxpass","subj":"T26920","obj":"T26921"},{"id":"R19059","pred":"ROOT","subj":"T26921","obj":"T26921"},{"id":"R19060","pred":"cc","subj":"T26922","obj":"T26921"},{"id":"R19061","pred":"conj","subj":"T26923","obj":"T26921"},{"id":"R19062","pred":"prep","subj":"T26924","obj":"T26923"},{"id":"R19063","pred":"amod","subj":"T26925","obj":"T26926"},{"id":"R19064","pred":"pobj","subj":"T26926","obj":"T26924"},{"id":"R19065","pred":"punct","subj":"T26927","obj":"T26921"},{"id":"R19066","pred":"det","subj":"T26928","obj":"T26930"},{"id":"R19067","pred":"amod","subj":"T26929","obj":"T26930"},{"id":"R19068","pred":"nsubj","subj":"T26930","obj":"T26936"},{"id":"R19069","pred":"punct","subj":"T26931","obj":"T26932"},{"id":"R19070","pred":"prep","subj":"T26932","obj":"T26930"},{"id":"R19071","pred":"det","subj":"T26933","obj":"T26934"},{"id":"R19072","pred":"pobj","subj":"T26934","obj":"T26932"},{"id":"R19073","pred":"punct","subj":"T26935","obj":"T26930"},{"id":"R19074","pred":"ROOT","subj":"T26936","obj":"T26936"},{"id":"R19075","pred":"det","subj":"T26937","obj":"T26938"},{"id":"R19076","pred":"dobj","subj":"T26938","obj":"T26936"},{"id":"R19077","pred":"prep","subj":"T26939","obj":"T26938"},{"id":"R19078","pred":"det","subj":"T26940","obj":"T26946"},{"id":"R19079","pred":"amod","subj":"T26941","obj":"T26946"},{"id":"R19080","pred":"nmod","subj":"T26942","obj":"T26946"},{"id":"R19081","pred":"punct","subj":"T26943","obj":"T26944"},{"id":"R19082","pred":"nmod","subj":"T26944","obj":"T26946"},{"id":"R19083","pred":"compound","subj":"T26945","obj":"T26946"},{"id":"R19084","pred":"pobj","subj":"T26946","obj":"T26939"},{"id":"R19085","pred":"punct","subj":"T26947","obj":"T26936"},{"id":"R19086","pred":"det","subj":"T26948","obj":"T26955"},{"id":"R19087","pred":"nsubj","subj":"T26949","obj":"T26955"},{"id":"R19088","pred":"punct","subj":"T26950","obj":"T26951"},{"id":"R19089","pred":"punct","subj":"T26951","obj":"T26953"},{"id":"R19090","pred":"punct","subj":"T26952","obj":"T26953"},{"id":"R19091","pred":"amod","subj":"T26953","obj":"T26954"},{"id":"R19092","pred":"nsubj","subj":"T26954","obj":"T26955"},{"id":"R19093","pred":"ROOT","subj":"T26955","obj":"T26955"},{"id":"R19094","pred":"preconj","subj":"T26956","obj":"T26959"},{"id":"R19095","pred":"det","subj":"T26957","obj":"T26959"},{"id":"R19096","pred":"nummod","subj":"T26958","obj":"T26959"},{"id":"R19097","pred":"dobj","subj":"T26959","obj":"T26955"},{"id":"R19098","pred":"cc","subj":"T26960","obj":"T26959"},{"id":"R19099","pred":"nummod","subj":"T26961","obj":"T26963"},{"id":"R19100","pred":"compound","subj":"T26962","obj":"T26963"},{"id":"R19101","pred":"conj","subj":"T26963","obj":"T26959"},{"id":"R19102","pred":"prep","subj":"T26964","obj":"T26963"},{"id":"R19103","pred":"compound","subj":"T26965","obj":"T26967"},{"id":"R19104","pred":"punct","subj":"T26966","obj":"T26967"},{"id":"R19105","pred":"pobj","subj":"T26967","obj":"T26964"},{"id":"R19106","pred":"punct","subj":"T26968","obj":"T26955"},{"id":"R19107","pred":"det","subj":"T26969","obj":"T26970"},{"id":"R19108","pred":"nsubj","subj":"T26970","obj":"T26971"},{"id":"R19109","pred":"ROOT","subj":"T26971","obj":"T26971"},{"id":"R19110","pred":"det","subj":"T26972","obj":"T26973"},{"id":"R19111","pred":"dobj","subj":"T26973","obj":"T26971"},{"id":"R19112","pred":"cc","subj":"T26974","obj":"T26973"},{"id":"R19113","pred":"conj","subj":"T26975","obj":"T26973"},{"id":"R19114","pred":"prep","subj":"T26976","obj":"T26975"},{"id":"R19115","pred":"advmod","subj":"T26977","obj":"T26978"},{"id":"R19116","pred":"advmod","subj":"T26978","obj":"T26979"},{"id":"R19117","pred":"nummod","subj":"T26979","obj":"T26981"},{"id":"R19118","pred":"amod","subj":"T26980","obj":"T26981"},{"id":"R19119","pred":"pobj","subj":"T26981","obj":"T26976"},{"id":"R19120","pred":"acl","subj":"T26982","obj":"T26981"},{"id":"R19121","pred":"prep","subj":"T26983","obj":"T26982"},{"id":"R19122","pred":"pobj","subj":"T26984","obj":"T26983"},{"id":"R19123","pred":"punct","subj":"T26985","obj":"T26971"},{"id":"R19124","pred":"punct","subj":"T26986","obj":"T26989"},{"id":"R19125","pred":"punct","subj":"T26987","obj":"T26989"},{"id":"R19126","pred":"punct","subj":"T26988","obj":"T26989"},{"id":"R19127","pred":"ROOT","subj":"T26989","obj":"T26989"},{"id":"R19128","pred":"compound","subj":"T26990","obj":"T26991"},{"id":"R19129","pred":"dobj","subj":"T26991","obj":"T26989"},{"id":"R19130","pred":"punct","subj":"T26992","obj":"T26991"},{"id":"R19131","pred":"amod","subj":"T26993","obj":"T26994"},{"id":"R19132","pred":"npadvmod","subj":"T26994","obj":"T26989"},{"id":"R19133","pred":"punct","subj":"T26995","obj":"T26989"},{"id":"R19134","pred":"punct","subj":"T26996","obj":"T26989"},{"id":"R18916","pred":"compound","subj":"T26778","obj":"T26779"},{"id":"R18917","pred":"pobj","subj":"T26779","obj":"T26777"},{"id":"R18918","pred":"advcl","subj":"T26780","obj":"T26767"},{"id":"R18919","pred":"nummod","subj":"T26781","obj":"T26782"},{"id":"R18920","pred":"dobj","subj":"T26782","obj":"T26780"},{"id":"R18921","pred":"punct","subj":"T26783","obj":"T26767"},{"id":"R18922","pred":"compound","subj":"T26784","obj":"T26788"},{"id":"R18923","pred":"compound","subj":"T26785","obj":"T26788"},{"id":"R18924","pred":"compound","subj":"T26786","obj":"T26787"},{"id":"R18925","pred":"compound","subj":"T26787","obj":"T26788"},{"id":"R18926","pred":"ROOT","subj":"T26788","obj":"T26788"},{"id":"R18927","pred":"acl","subj":"T26789","obj":"T26788"},{"id":"R18928","pred":"prep","subj":"T26790","obj":"T26789"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"pmc-enju-pas","denotations":[{"id":"T26385","span":{"begin":2053,"end":2056},"obj":"DT"},{"id":"T26384","span":{"begin":2044,"end":2051},"obj":"NN"},{"id":"T26383","span":{"begin":2027,"end":2043},"obj":"NN"},{"id":"T26382","span":{"begin":2019,"end":2026},"obj":"NN"},{"id":"T26381","span":{"begin":2008,"end":2018},"obj":"JJ"},{"id":"T26380","span":{"begin":2004,"end":2007},"obj":"DT"},{"id":"T26379","span":{"begin":2001,"end":2003},"obj":"IN"},{"id":"T26378","span":{"begin":1990,"end":2000},"obj":"NN"},{"id":"T26377","span":{"begin":1986,"end":1989},"obj":"DT"},{"id":"T26376","span":{"begin":1977,"end":1985},"obj":"VB"},{"id":"T26375","span":{"begin":1975,"end":1976},"obj":"-RRB-"},{"id":"T26374","span":{"begin":1969,"end":1975},"obj":"NN"},{"id":"T26373","span":{"begin":1965,"end":1968},"obj":"DT"},{"id":"T26372","span":{"begin":1959,"end":1964},"obj":"IN"},{"id":"T26371","span":{"begin":1958,"end":1959},"obj":"-LRB-"},{"id":"T26370","span":{"begin":1953,"end":1957},"obj":"NN"},{"id":"T26369","span":{"begin":1945,"end":1952},"obj":"JJ"},{"id":"T26368","span":{"begin":1941,"end":1944},"obj":"DT"},{"id":"T26367","span":{"begin":1931,"end":1939},"obj":"NN"},{"id":"T26366","span":{"begin":1915,"end":1930},"obj":"JJ"},{"id":"T26365","span":{"begin":1912,"end":1914},"obj":"TO"},{"id":"T26364","span":{"begin":1901,"end":1911},"obj":"VB"},{"id":"T26363","span":{"begin":1897,"end":1900},"obj":"CC"},{"id":"T26362","span":{"begin":1886,"end":1896},"obj":"VB"},{"id":"T26361","span":{"begin":1882,"end":1885},"obj":"VB"},{"id":"T26360","span":{"begin":1873,"end":1881},"obj":"NN"},{"id":"T26359","span":{"begin":1862,"end":1872},"obj":"NN"},{"id":"T26358","span":{"begin":1857,"end":1860},"obj":"NN"},{"id":"T26357","span":{"begin":1855,"end":1856},"obj":"CD"},{"id":"T26356","span":{"begin":1851,"end":1854},"obj":"IN"},{"id":"T26355","span":{"begin":1847,"end":1850},"obj":"NN"},{"id":"T26354","span":{"begin":1842,"end":1846},"obj":"IN"},{"id":"T26353","span":{"begin":1831,"end":1841},"obj":"VB"},{"id":"T26352","span":{"begin":1827,"end":1830},"obj":"CC"},{"id":"T26351","span":{"begin":1825,"end":1826},"obj":"-COMMA-"},{"id":"T26350","span":{"begin":1817,"end":1825},"obj":"NN"},{"id":"T26349","span":{"begin":1808,"end":1816},"obj":"NN"},{"id":"T26348","span":{"begin":1792,"end":1807},"obj":"JJ"},{"id":"T26347","span":{"begin":1788,"end":1791},"obj":"CC"},{"id":"T26346","span":{"begin":1779,"end":1787},"obj":"NN"},{"id":"T26345","span":{"begin":1759,"end":1778},"obj":"NN"},{"id":"T26344","span":{"begin":1750,"end":1758},"obj":"NN"},{"id":"T26343","span":{"begin":1740,"end":1749},"obj":"NN"},{"id":"T26342","span":{"begin":1736,"end":1739},"obj":"DT"},{"id":"T26341","span":{"begin":1731,"end":1735},"obj":"IN"},{"id":"T26340","span":{"begin":1725,"end":1730},"obj":"IN"},{"id":"T26339","span":{"begin":1723,"end":1724},"obj":"-COMMA-"},{"id":"T26338","span":{"begin":1716,"end":1723},"obj":"NN"},{"id":"T26337","span":{"begin":1699,"end":1715},"obj":"NN"},{"id":"T26336","span":{"begin":1691,"end":1698},"obj":"NN"},{"id":"T26335","span":{"begin":1681,"end":1690},"obj":"VB"},{"id":"T26334","span":{"begin":1677,"end":1680},"obj":"DT"},{"id":"T26333","span":{"begin":1674,"end":1676},"obj":"CC"},{"id":"T26332","span":{"begin":1666,"end":1673},"obj":"NN"},{"id":"T26331","span":{"begin":1656,"end":1665},"obj":"JJ"},{"id":"T26330","span":{"begin":1647,"end":1655},"obj":"VB"},{"id":"T26329","span":{"begin":1638,"end":1646},"obj":"NN"},{"id":"T26328","span":{"begin":1627,"end":1637},"obj":"NN"},{"id":"T26327","span":{"begin":1622,"end":1626},"obj":"IN"},{"id":"T26326","span":{"begin":1607,"end":1621},"obj":"VB"},{"id":"T26325","span":{"begin":1602,"end":1606},"obj":"VB"},{"id":"T26324","span":{"begin":1597,"end":1601},"obj":"NN"},{"id":"T26323","span":{"begin":1588,"end":1596},"obj":"NN"},{"id":"T26322","span":{"begin":1578,"end":1586},"obj":"NN"},{"id":"T26321","span":{"begin":1569,"end":1577},"obj":"NN"},{"id":"T26320","span":{"begin":1560,"end":1568},"obj":"NN"},{"id":"T26319","span":{"begin":1545,"end":1559},"obj":"JJ"},{"id":"T26318","span":{"begin":1535,"end":1544},"obj":"VB"},{"id":"T26317","span":{"begin":1524,"end":1534},"obj":"RB"},{"id":"T26316","span":{"begin":1521,"end":1523},"obj":"CD"},{"id":"T26315","span":{"begin":1514,"end":1520},"obj":"NN"},{"id":"T26314","span":{"begin":1511,"end":1513},"obj":"IN"},{"id":"T26313","span":{"begin":1503,"end":1510},"obj":"NN"},{"id":"T26312","span":{"begin":1500,"end":1502},"obj":"IN"},{"id":"T26311","span":{"begin":1484,"end":1499},"obj":"NN"},{"id":"T26310","span":{"begin":1482,"end":1483},"obj":"-RRB-"},{"id":"T26309","span":{"begin":1481,"end":1482},"obj":"NN"},{"id":"T26308","span":{"begin":1480,"end":1481},"obj":"-LRB-"},{"id":"T26307","span":{"begin":1473,"end":1479},"obj":"NN"},{"id":"T26306","span":{"begin":1456,"end":1472},"obj":"NN"},{"id":"T26305","span":{"begin":1452,"end":1455},"obj":"CC"},{"id":"T26304","span":{"begin":1442,"end":1451},"obj":"NN"},{"id":"T26303","span":{"begin":1437,"end":1441},"obj":"NN"},{"id":"T26302","span":{"begin":1427,"end":1436},"obj":"VB"},{"id":"T26301","span":{"begin":1417,"end":1426},"obj":"JJ"},{"id":"T26300","span":{"begin":1412,"end":1416},"obj":"NN"},{"id":"T26299","span":{"begin":1404,"end":1411},"obj":"NN"},{"id":"T26298","span":{"begin":1402,"end":1403},"obj":"-RRB-"},{"id":"T26297","span":{"begin":1401,"end":1402},"obj":"NN"},{"id":"T26296","span":{"begin":1400,"end":1401},"obj":"-LRB-"},{"id":"T26295","span":{"begin":1390,"end":1399},"obj":"NN"},{"id":"T26294","span":{"begin":1374,"end":1389},"obj":"JJ"},{"id":"T26293","span":{"begin":1371,"end":1373},"obj":"TO"},{"id":"T26292","span":{"begin":1360,"end":1370},"obj":"VB"},{"id":"T26291","span":{"begin":1354,"end":1359},"obj":"NN"},{"id":"T26290","span":{"begin":1343,"end":1353},"obj":"NN"},{"id":"T26289","span":{"begin":1339,"end":1342},"obj":"CC"},{"id":"T26288","span":{"begin":1337,"end":1338},"obj":"-COMMA-"},{"id":"T26287","span":{"begin":1329,"end":1337},"obj":"NN"},{"id":"T26286","span":{"begin":1320,"end":1328},"obj":"NN"},{"id":"T26285","span":{"begin":1304,"end":1319},"obj":"JJ"},{"id":"T26284","span":{"begin":1300,"end":1303},"obj":"CC"},{"id":"T26283","span":{"begin":1291,"end":1299},"obj":"NN"},{"id":"T26282","span":{"begin":1271,"end":1290},"obj":"NN"},{"id":"T26281","span":{"begin":1262,"end":1270},"obj":"NN"},{"id":"T26280","span":{"begin":1252,"end":1261},"obj":"NN"},{"id":"T26279","span":{"begin":1248,"end":1251},"obj":"DT"},{"id":"T26278","span":{"begin":1243,"end":1247},"obj":"IN"},{"id":"T26277","span":{"begin":1228,"end":1242},"obj":"VB"},{"id":"T26276","span":{"begin":1223,"end":1227},"obj":"VB"},{"id":"T26275","span":{"begin":1217,"end":1222},"obj":"NN"},{"id":"T26274","span":{"begin":1214,"end":1215},"obj":"-RRB-"},{"id":"T26273","span":{"begin":1209,"end":1214},"obj":"NN"},{"id":"T26272","span":{"begin":1205,"end":1208},"obj":"CD"},{"id":"T26271","span":{"begin":1204,"end":1205},"obj":"-LRB-"},{"id":"T26270","span":{"begin":1200,"end":1203},"obj":"NN"},{"id":"T26269","span":{"begin":1197,"end":1199},"obj":"IN"},{"id":"T26268","span":{"begin":1189,"end":1196},"obj":"NN"},{"id":"T26267","span":{"begin":1186,"end":1188},"obj":"CC"},{"id":"T26266","span":{"begin":1177,"end":1185},"obj":"NN"},{"id":"T26265","span":{"begin":1173,"end":1176},"obj":"DT"},{"id":"T26264","span":{"begin":1170,"end":1172},"obj":"IN"},{"id":"T26263","span":{"begin":1161,"end":1169},"obj":"NN"},{"id":"T26262","span":{"begin":1141,"end":1160},"obj":"NN"},{"id":"T26261","span":{"begin":1132,"end":1140},"obj":"NN"},{"id":"T26260","span":{"begin":1122,"end":1131},"obj":"NN"},{"id":"T26259","span":{"begin":1118,"end":1121},"obj":"DT"},{"id":"T26258","span":{"begin":1107,"end":1117},"obj":"VB"},{"id":"T26257","span":{"begin":1102,"end":1106},"obj":"NN"},{"id":"T26256","span":{"begin":1093,"end":1101},"obj":"NNP"},{"id":"T26255","span":{"begin":1089,"end":1092},"obj":"CC"},{"id":"T26254","span":{"begin":1080,"end":1088},"obj":"JJ"},{"id":"T26253","span":{"begin":1077,"end":1079},"obj":"IN"},{"id":"T26252","span":{"begin":1068,"end":1076},"obj":"VB"},{"id":"T26251","span":{"begin":1059,"end":1067},"obj":"NN"},{"id":"T26250","span":{"begin":1050,"end":1058},"obj":"NN"},{"id":"T26249","span":{"begin":1045,"end":1049},"obj":"NN"},{"id":"T26248","span":{"begin":1036,"end":1044},"obj":"NN"},{"id":"T26247","span":{"begin":1029,"end":1035},"obj":"JJ"},{"id":"T26246","span":{"begin":1023,"end":1027},"obj":"NN"},{"id":"T26245","span":{"begin":1014,"end":1022},"obj":"NN"},{"id":"T26244","span":{"begin":1011,"end":1013},"obj":"IN"},{"id":"T26243","span":{"begin":1007,"end":1010},"obj":"RB"},{"id":"T26242","span":{"begin":1003,"end":1006},"obj":"CC"},{"id":"T26241","span":{"begin":998,"end":1002},"obj":"NN"},{"id":"T26240","span":{"begin":989,"end":997},"obj":"JJ"},{"id":"T26239","span":{"begin":974,"end":988},"obj":"JJ"},{"id":"T26238","span":{"begin":971,"end":973},"obj":"IN"},{"id":"T26237","span":{"begin":961,"end":970},"obj":"VB"},{"id":"T26236","span":{"begin":947,"end":960},"obj":"RB"},{"id":"T26235","span":{"begin":944,"end":946},"obj":"VB"},{"id":"T26234","span":{"begin":935,"end":943},"obj":"NN"},{"id":"T26233","span":{"begin":926,"end":934},"obj":"JJ"},{"id":"T26232","span":{"begin":917,"end":925},"obj":"NN"},{"id":"T26231","span":{"begin":913,"end":916},"obj":"DT"},{"id":"T26230","span":{"begin":911,"end":912},"obj":"-RRB-"},{"id":"T26229","span":{"begin":910,"end":911},"obj":"NN"},{"id":"T26228","span":{"begin":909,"end":910},"obj":"-LRB-"},{"id":"T26227","span":{"begin":899,"end":907},"obj":"NN"},{"id":"T26226","span":{"begin":890,"end":898},"obj":"NN"},{"id":"T26225","span":{"begin":881,"end":889},"obj":"JJ"},{"id":"T26224","span":{"begin":872,"end":880},"obj":"NN"},{"id":"T26223","span":{"begin":868,"end":871},"obj":"IN"},{"id":"T26222","span":{"begin":858,"end":867},"obj":"JJ"},{"id":"T26221","span":{"begin":855,"end":857},"obj":"VB"},{"id":"T26220","span":{"begin":847,"end":854},"obj":"NN"},{"id":"T26425","span":{"begin":2275,"end":2276},"obj":"-RRB-"},{"id":"T26424","span":{"begin":2270,"end":2275},"obj":"NN"},{"id":"T26423","span":{"begin":2262,"end":2269},"obj":"JJ"},{"id":"T26422","span":{"begin":2260,"end":2261},"obj":"-COMMA-"},{"id":"T26421","span":{"begin":2256,"end":2260},"obj":"CD"},{"id":"T26420","span":{"begin":2255,"end":2256},"obj":"SYM"},{"id":"T26419","span":{"begin":2254,"end":2255},"obj":"NN"},{"id":"T26418","span":{"begin":2252,"end":2253},"obj":"-COMMA-"},{"id":"T26417","span":{"begin":2251,"end":2252},"obj":"SYM"},{"id":"T26416","span":{"begin":2250,"end":2251},"obj":"-LRB-"},{"id":"T26415","span":{"begin":2238,"end":2249},"obj":"FW"},{"id":"T26414","span":{"begin":2235,"end":2237},"obj":"IN"},{"id":"T26413","span":{"begin":2225,"end":2234},"obj":"VB"},{"id":"T26412","span":{"begin":2213,"end":2224},"obj":"NN"},{"id":"T26411","span":{"begin":2201,"end":2212},"obj":"JJ"},{"id":"T26410","span":{"begin":2195,"end":2200},"obj":"CD"},{"id":"T26409","span":{"begin":2189,"end":2194},"obj":"JJ"},{"id":"T26408","span":{"begin":2186,"end":2188},"obj":"IN"},{"id":"T26407","span":{"begin":2183,"end":2185},"obj":"IN"},{"id":"T26406","span":{"begin":2179,"end":2182},"obj":"NN"},{"id":"T26405","span":{"begin":2175,"end":2178},"obj":"CC"},{"id":"T26404","span":{"begin":2170,"end":2174},"obj":"NN"},{"id":"T26403","span":{"begin":2166,"end":2169},"obj":"DT"},{"id":"T26402","span":{"begin":2156,"end":2165},"obj":"VB"},{"id":"T26401","span":{"begin":2148,"end":2155},"obj":"NN"},{"id":"T26400","span":{"begin":2144,"end":2147},"obj":"DT"},{"id":"T26399","span":{"begin":2135,"end":2142},"obj":"NN"},{"id":"T26398","span":{"begin":2132,"end":2134},"obj":"IN"},{"id":"T26397","span":{"begin":2123,"end":2131},"obj":"NN"},{"id":"T26396","span":{"begin":2119,"end":2122},"obj":"NN"},{"id":"T26395","span":{"begin":2116,"end":2118},"obj":"CD"},{"id":"T26394","span":{"begin":2112,"end":2115},"obj":"CC"},{"id":"T26393","span":{"begin":2108,"end":2111},"obj":"NN"},{"id":"T26392","span":{"begin":2105,"end":2107},"obj":"CD"},{"id":"T26391","span":{"begin":2101,"end":2104},"obj":"DT"},{"id":"T26390","span":{"begin":2096,"end":2100},"obj":"CC"},{"id":"T26389","span":{"begin":2088,"end":2095},"obj":"VB"},{"id":"T26388","span":{"begin":2083,"end":2087},"obj":"NN"},{"id":"T26387","span":{"begin":2070,"end":2082},"obj":"JJ"},{"id":"T26386","span":{"begin":2057,"end":2069},"obj":"NN"},{"id":"T15906","span":{"begin":793,"end":801},"obj":"NN"},{"id":"T15905","span":{"begin":784,"end":792},"obj":"NN"},{"id":"T15904","span":{"begin":775,"end":783},"obj":"JJ"},{"id":"T15903","span":{"begin":766,"end":774},"obj":"NN"},{"id":"T15902","span":{"begin":754,"end":765},"obj":"JJ"},{"id":"T15901","span":{"begin":750,"end":753},"obj":"IN"},{"id":"T15900","span":{"begin":741,"end":749},"obj":"VB"},{"id":"T15899","span":{"begin":738,"end":740},"obj":"VB"},{"id":"T15898","span":{"begin":730,"end":737},"obj":"NN"},{"id":"T15897","span":{"begin":719,"end":729},"obj":"JJ"},{"id":"T15896","span":{"begin":714,"end":718},"obj":"IN"},{"id":"T15895","span":{"begin":703,"end":713},"obj":"VB"},{"id":"T15894","span":{"begin":701,"end":702},"obj":"-COMMA-"},{"id":"T15893","span":{"begin":700,"end":701},"obj":"-RRB-"},{"id":"T15892","span":{"begin":698,"end":700},"obj":"NN"},{"id":"T15891","span":{"begin":693,"end":697},"obj":"NNP"},{"id":"T15890","span":{"begin":692,"end":693},"obj":"-LRB-"},{"id":"T15889","span":{"begin":687,"end":691},"obj":"NN"},{"id":"T15888","span":{"begin":678,"end":686},"obj":"NN"},{"id":"T15887","span":{"begin":675,"end":677},"obj":"IN"},{"id":"T15886","span":{"begin":665,"end":674},"obj":"VB"},{"id":"T15885","span":{"begin":661,"end":664},"obj":"CC"},{"id":"T15884","span":{"begin":656,"end":660},"obj":"NN"},{"id":"T15883","span":{"begin":647,"end":655},"obj":"JJ"},{"id":"T15882","span":{"begin":644,"end":646},"obj":"IN"},{"id":"T15881","span":{"begin":634,"end":643},"obj":"VB"},{"id":"T15880","span":{"begin":620,"end":633},"obj":"RB"},{"id":"T15879","span":{"begin":616,"end":619},"obj":"VB"},{"id":"T15878","span":{"begin":607,"end":615},"obj":"NN"},{"id":"T15877","span":{"begin":598,"end":606},"obj":"NN"},{"id":"T15876","span":{"begin":589,"end":597},"obj":"NN"},{"id":"T15875","span":{"begin":574,"end":588},"obj":"JJ"},{"id":"T15874","span":{"begin":569,"end":573},"obj":"IN"},{"id":"T15873","span":{"begin":563,"end":568},"obj":"VB"},{"id":"T15872","span":{"begin":560,"end":562},"obj":"PRP"},{"id":"T15871","span":{"begin":554,"end":558},"obj":"NN"},{"id":"T15870","span":{"begin":545,"end":553},"obj":"NNP"},{"id":"T15869","span":{"begin":541,"end":544},"obj":"CC"},{"id":"T15868","span":{"begin":532,"end":540},"obj":"NNP"},{"id":"T15867","span":{"begin":527,"end":531},"obj":"IN"},{"id":"T15866","span":{"begin":517,"end":526},"obj":"NN"},{"id":"T15865","span":{"begin":508,"end":516},"obj":"NN"},{"id":"T15864","span":{"begin":497,"end":507},"obj":"NN"},{"id":"T15863","span":{"begin":488,"end":496},"obj":"NN"},{"id":"T15862","span":{"begin":481,"end":487},"obj":"JJ"},{"id":"T15861","span":{"begin":471,"end":480},"obj":"NN"},{"id":"T15860","span":{"begin":467,"end":470},"obj":"DT"},{"id":"T15859","span":{"begin":464,"end":466},"obj":"IN"},{"id":"T15858","span":{"begin":451,"end":463},"obj":"NN"},{"id":"T15857","span":{"begin":448,"end":450},"obj":"IN"},{"id":"T15856","span":{"begin":439,"end":447},"obj":"VB"},{"id":"T15855","span":{"begin":435,"end":438},"obj":"VB"},{"id":"T15854","span":{"begin":426,"end":434},"obj":"NN"},{"id":"T15853","span":{"begin":417,"end":425},"obj":"JJ"},{"id":"T15852","span":{"begin":408,"end":416},"obj":"NN"},{"id":"T15851","span":{"begin":404,"end":407},"obj":"DT"},{"id":"T15850","span":{"begin":399,"end":403},"obj":"IN"},{"id":"T15849","span":{"begin":385,"end":398},"obj":"NN"},{"id":"T15848","span":{"begin":378,"end":384},"obj":"VB"},{"id":"T15847","span":{"begin":375,"end":377},"obj":"TO"},{"id":"T15846","span":{"begin":367,"end":374},"obj":"NN"},{"id":"T15845","span":{"begin":356,"end":366},"obj":"JJ"},{"id":"T15844","span":{"begin":353,"end":355},"obj":"IN"},{"id":"T15843","span":{"begin":345,"end":352},"obj":"NN"},{"id":"T15842","span":{"begin":341,"end":344},"obj":"DT"},{"id":"T15841","span":{"begin":336,"end":339},"obj":"NN"},{"id":"T15840","span":{"begin":333,"end":335},"obj":"TO"},{"id":"T15839","span":{"begin":324,"end":332},"obj":"NN"},{"id":"T15838","span":{"begin":321,"end":323},"obj":"IN"},{"id":"T15837","span":{"begin":315,"end":320},"obj":"NN"},{"id":"T15836","span":{"begin":312,"end":314},"obj":"IN"},{"id":"T15835","span":{"begin":303,"end":311},"obj":"NN"},{"id":"T15834","span":{"begin":294,"end":302},"obj":"NN"},{"id":"T15833","span":{"begin":290,"end":293},"obj":"DT"},{"id":"T15832","span":{"begin":285,"end":289},"obj":"IN"},{"id":"T15831","span":{"begin":271,"end":284},"obj":"NN"},{"id":"T15830","span":{"begin":263,"end":270},"obj":"VB"},{"id":"T15829","span":{"begin":259,"end":262},"obj":"IN"},{"id":"T15828","span":{"begin":252,"end":258},"obj":"NN"},{"id":"T15827","span":{"begin":242,"end":251},"obj":"JJ"},{"id":"T15826","span":{"begin":239,"end":241},"obj":"DT"},{"id":"T15825","span":{"begin":235,"end":238},"obj":"VB"},{"id":"T15824","span":{"begin":227,"end":234},"obj":"NN"},{"id":"T15823","span":{"begin":219,"end":226},"obj":"IN"},{"id":"T15822","span":{"begin":216,"end":218},"obj":"IN"},{"id":"T15821","span":{"begin":207,"end":215},"obj":"NN"},{"id":"T15820","span":{"begin":203,"end":206},"obj":"DT"},{"id":"T15819","span":{"begin":195,"end":202},"obj":"VB"},{"id":"T15818","span":{"begin":192,"end":194},"obj":"TO"},{"id":"T15817","span":{"begin":185,"end":191},"obj":"VB"},{"id":"T15816","span":{"begin":182,"end":184},"obj":"PRP"},{"id":"T15815","span":{"begin":180,"end":181},"obj":"-COMMA-"},{"id":"T15814","span":{"begin":176,"end":180},"obj":"FW"},{"id":"T15813","span":{"begin":173,"end":175},"obj":"FW"},{"id":"T15812","span":{"begin":169,"end":172},"obj":"CC"},{"id":"T15811","span":{"begin":163,"end":168},"obj":"FW"},{"id":"T15810","span":{"begin":160,"end":162},"obj":"FW"},{"id":"T15809","span":{"begin":155,"end":159},"obj":"CC"},{"id":"T15808","span":{"begin":148,"end":154},"obj":"NN"},{"id":"T15807","span":{"begin":134,"end":147},"obj":"JJ"},{"id":"T15806","span":{"begin":131,"end":133},"obj":"DT"},{"id":"T15805","span":{"begin":128,"end":130},"obj":"IN"},{"id":"T15804","span":{"begin":119,"end":127},"obj":"NN"},{"id":"T15803","span":{"begin":110,"end":118},"obj":"JJ"},{"id":"T15802","span":{"begin":101,"end":109},"obj":"NN"},{"id":"T15801","span":{"begin":97,"end":100},"obj":"DT"},{"id":"T15800","span":{"begin":94,"end":96},"obj":"TO"},{"id":"T15799","span":{"begin":88,"end":93},"obj":"VB"},{"id":"T15798","span":{"begin":80,"end":87},"obj":"NN"},{"id":"T15797","span":{"begin":74,"end":79},"obj":"IN"},{"id":"T15796","span":{"begin":65,"end":73},"obj":"NN"},{"id":"T15795","span":{"begin":56,"end":64},"obj":"NN"},{"id":"T15794","span":{"begin":47,"end":55},"obj":"JJ"},{"id":"T15793","span":{"begin":38,"end":46},"obj":"NN"},{"id":"T15792","span":{"begin":31,"end":37},"obj":"NNP"},{"id":"T15791","span":{"begin":17,"end":30},"obj":"JJ"},{"id":"T15790","span":{"begin":8,"end":16},"obj":"VB"},{"id":"T15789","span":{"begin":0,"end":7},"obj":"NN"}],"relations":[{"id":"R11620","pred":"arg1Of","subj":"T15789","obj":"T15790"},{"id":"R11621","pred":"arg2Of","subj":"T15796","obj":"T15790"},{"id":"R11622","pred":"arg1Of","subj":"T15796","obj":"T15791"},{"id":"R11623","pred":"arg1Of","subj":"T15796","obj":"T15792"},{"id":"R11624","pred":"arg1Of","subj":"T15796","obj":"T15793"},{"id":"R11625","pred":"arg1Of","subj":"T15796","obj":"T15794"},{"id":"R11626","pred":"arg1Of","subj":"T15796","obj":"T15795"},{"id":"R11627","pred":"arg1Of","subj":"T15798","obj":"T15799"},{"id":"R11628","pred":"arg2Of","subj":"T15799","obj":"T15797"},{"id":"R11629","pred":"arg1Of","subj":"T15799","obj":"T15800"},{"id":"R11630","pred":"arg1Of","subj":"T15799","obj":"T15805"},{"id":"R11631","pred":"arg1Of","subj":"T15799","obj":"T15811"},{"id":"R11632","pred":"arg1Of","subj":"T15799","obj":"T15814"},{"id":"R11633","pred":"arg2Of","subj":"T15804","obj":"T15800"},{"id":"R11634","pred":"arg1Of","subj":"T15804","obj":"T15801"},{"id":"R11635","pred":"arg1Of","subj":"T15804","obj":"T15802"},{"id":"R11636","pred":"arg1Of","subj":"T15804","obj":"T15803"},{"id":"R11637","pred":"arg2Of","subj":"T15808","obj":"T15805"},{"id":"R11638","pred":"arg1Of","subj":"T15808","obj":"T15806"},{"id":"R11639","pred":"arg1Of","subj":"T15808","obj":"T15807"},{"id":"R11640","pred":"arg1Of","subj":"T15811","obj":"T15810"},{"id":"R11641","pred":"arg1Of","subj":"T15811","obj":"T15812"},{"id":"R11642","pred":"arg1Of","subj":"T15812","obj":"T15809"},{"id":"R11643","pred":"arg2Of","subj":"T15814","obj":"T15812"},{"id":"R11644","pred":"arg1Of","subj":"T15814","obj":"T15813"},{"id":"R11645","pred":"arg1Of","subj":"T15816","obj":"T15817"},{"id":"R11646","pred":"arg1Of","subj":"T15816","obj":"T15819"},{"id":"R11647","pred":"arg1Of","subj":"T15817","obj":"T15797"},{"id":"R11648","pred":"arg1Of","subj":"T15817","obj":"T15815"},{"id":"R11649","pred":"arg2Of","subj":"T15819","obj":"T15817"},{"id":"R11650","pred":"arg1Of","subj":"T15819","obj":"T15818"},{"id":"R11651","pred":"arg2Of","subj":"T15821","obj":"T15819"},{"id":"R11652","pred":"arg1Of","subj":"T15821","obj":"T15820"},{"id":"R11653","pred":"arg1Of","subj":"T15821","obj":"T15822"},{"id":"R11654","pred":"arg1Of","subj":"T15824","obj":"T15825"},{"id":"R11655","pred":"arg2Of","subj":"T15825","obj":"T15822"},{"id":"R11656","pred":"arg1Of","subj":"T15825","obj":"T15823"},{"id":"R11657","pred":"arg2Of","subj":"T15828","obj":"T15825"},{"id":"R11658","pred":"arg1Of","subj":"T15828","obj":"T15826"},{"id":"R11659","pred":"arg1Of","subj":"T15828","obj":"T15827"},{"id":"R11660","pred":"arg1Of","subj":"T15828","obj":"T15829"},{"id":"R11661","pred":"arg2Of","subj":"T15830","obj":"T15829"},{"id":"R11662","pred":"arg2Of","subj":"T15831","obj":"T15830"},{"id":"R11663","pred":"arg1Of","subj":"T15831","obj":"T15832"},{"id":"R11664","pred":"arg1Of","subj":"T15831","obj":"T15836"},{"id":"R11665","pred":"arg2Of","subj":"T15835","obj":"T15832"},{"id":"R11666","pred":"arg1Of","subj":"T15835","obj":"T15833"},{"id":"R11667","pred":"arg1Of","subj":"T15835","obj":"T15834"},{"id":"R11668","pred":"arg2Of","subj":"T15837","obj":"T15836"},{"id":"R11669","pred":"arg1Of","subj":"T15837","obj":"T15838"},{"id":"R11670","pred":"arg2Of","subj":"T15839","obj":"T15838"},{"id":"R11671","pred":"arg1Of","subj":"T15839","obj":"T15840"},{"id":"R11672","pred":"arg2Of","subj":"T15841","obj":"T15840"},{"id":"R11673","pred":"arg1Of","subj":"T15843","obj":"T15842"},{"id":"R11674","pred":"arg1Of","subj":"T15843","obj":"T15844"},{"id":"R11675","pred":"modOf","subj":"T15843","obj":"T15847"},{"id":"R11676","pred":"arg1Of","subj":"T15843","obj":"T15855"},{"id":"R11677","pred":"arg2Of","subj":"T15843","obj":"T15856"},{"id":"R11678","pred":"arg2Of","subj":"T15846","obj":"T15844"},{"id":"R11679","pred":"arg1Of","subj":"T15846","obj":"T15845"},{"id":"R11680","pred":"arg1Of","subj":"T15848","obj":"T15847"},{"id":"R11681","pred":"arg2Of","subj":"T15849","obj":"T15848"},{"id":"R11682","pred":"arg1Of","subj":"T15849","obj":"T15850"},{"id":"R11683","pred":"arg2Of","subj":"T15854","obj":"T15850"},{"id":"R11684","pred":"arg1Of","subj":"T15854","obj":"T15851"},{"id":"R11685","pred":"arg1Of","subj":"T15854","obj":"T15852"},{"id":"R11686","pred":"arg1Of","subj":"T15854","obj":"T15853"},{"id":"R11687","pred":"arg2Of","subj":"T15856","obj":"T15855"},{"id":"R11688","pred":"arg1Of","subj":"T15858","obj":"T15856"},{"id":"R11689","pred":"arg2Of","subj":"T15858","obj":"T15857"},{"id":"R11690","pred":"arg1Of","subj":"T15858","obj":"T15859"},{"id":"R11691","pred":"arg1Of","subj":"T15858","obj":"T15867"},{"id":"R11692","pred":"arg2Of","subj":"T15866","obj":"T15859"},{"id":"R11693","pred":"arg1Of","subj":"T15866","obj":"T15860"},{"id":"R11694","pred":"arg1Of","subj":"T15866","obj":"T15861"},{"id":"R11695","pred":"arg1Of","subj":"T15866","obj":"T15862"},{"id":"R11696","pred":"arg1Of","subj":"T15866","obj":"T15863"},{"id":"R11697","pred":"arg1Of","subj":"T15866","obj":"T15864"},{"id":"R11698","pred":"arg1Of","subj":"T15866","obj":"T15865"},{"id":"R11699","pred":"arg1Of","subj":"T15868","obj":"T15869"},{"id":"R11700","pred":"arg2Of","subj":"T15870","obj":"T15869"},{"id":"R11701","pred":"arg2Of","subj":"T15871","obj":"T15867"},{"id":"R11702","pred":"arg1Of","subj":"T15871","obj":"T15868"},{"id":"R11703","pred":"arg1Of","subj":"T15871","obj":"T15870"},{"id":"R11704","pred":"arg1Of","subj":"T15872","obj":"T15873"},{"id":"R11705","pred":"arg1Of","subj":"T15873","obj":"T15894"},{"id":"R11706","pred":"modOf","subj":"T15873","obj":"T15895"},{"id":"R11707","pred":"arg1Of","subj":"T15878","obj":"T15875"},{"id":"R11708","pred":"arg1Of","subj":"T15878","obj":"T15876"},{"id":"R11709","pred":"arg1Of","subj":"T15878","obj":"T15877"},{"id":"R11710","pred":"arg1Of","subj":"T15878","obj":"T15879"},{"id":"R11711","pred":"arg2Of","subj":"T15878","obj":"T15881"},{"id":"R11712","pred":"arg1Of","subj":"T15878","obj":"T15886"},{"id":"R11713","pred":"arg2Of","subj":"T15881","obj":"T15879"},{"id":"R11714","pred":"arg1Of","subj":"T15881","obj":"T15880"},{"id":"R11715","pred":"arg1Of","subj":"T15881","obj":"T15882"},{"id":"R11716","pred":"arg1Of","subj":"T15881","obj":"T15885"},{"id":"R11717","pred":"arg2Of","subj":"T15884","obj":"T15882"},{"id":"R11718","pred":"arg1Of","subj":"T15884","obj":"T15883"},{"id":"R11719","pred":"arg2Of","subj":"T15885","obj":"T15873"},{"id":"R11720","pred":"arg1Of","subj":"T15885","obj":"T15874"},{"id":"R11721","pred":"arg2Of","subj":"T15886","obj":"T15885"},{"id":"R11722","pred":"arg1Of","subj":"T15886","obj":"T15887"},{"id":"R11723","pred":"arg2Of","subj":"T15889","obj":"T15887"},{"id":"R11724","pred":"arg1Of","subj":"T15889","obj":"T15888"},{"id":"R11725","pred":"arg2Of","subj":"T15892","obj":"T15886"},{"id":"R11726","pred":"arg2Of","subj":"T15892","obj":"T15890"},{"id":"R11727","pred":"arg1Of","subj":"T15892","obj":"T15891"},{"id":"R11728","pred":"arg3Of","subj":"T15893","obj":"T15890"},{"id":"R11729","pred":"arg1Of","subj":"T15898","obj":"T15897"},{"id":"R11730","pred":"arg1Of","subj":"T15898","obj":"T15899"},{"id":"R11731","pred":"arg2Of","subj":"T15898","obj":"T15900"},{"id":"R11732","pred":"arg2Of","subj":"T15900","obj":"T15895"},{"id":"R11733","pred":"arg1Of","subj":"T15900","obj":"T15896"},{"id":"R11734","pred":"arg2Of","subj":"T15900","obj":"T15899"},{"id":"R11735","pred":"arg1Of","subj":"T15900","obj":"T15901"},{"id":"R11736","pred":"arg2Of","subj":"T15906","obj":"T15901"},{"id":"R11737","pred":"arg1Of","subj":"T15906","obj":"T15902"},{"id":"R11738","pred":"arg1Of","subj":"T15906","obj":"T15903"},{"id":"R11739","pred":"arg1Of","subj":"T15906","obj":"T15904"},{"id":"R11740","pred":"arg1Of","subj":"T15906","obj":"T15905"},{"id":"R18586","pred":"arg1Of","subj":"T26220","obj":"T26221"},{"id":"R18587","pred":"arg1Of","subj":"T26220","obj":"T26222"},{"id":"R18588","pred":"arg2Of","subj":"T26222","obj":"T26221"},{"id":"R18589","pred":"arg1Of","subj":"T26222","obj":"T26223"},{"id":"R18590","pred":"arg2Of","subj":"T26227","obj":"T26223"},{"id":"R18591","pred":"arg1Of","subj":"T26227","obj":"T26224"},{"id":"R18592","pred":"arg1Of","subj":"T26227","obj":"T26225"},{"id":"R18593","pred":"arg1Of","subj":"T26227","obj":"T26226"},{"id":"R18594","pred":"arg2Of","subj":"T26229","obj":"T26228"},{"id":"R18595","pred":"arg3Of","subj":"T26230","obj":"T26228"},{"id":"R18596","pred":"arg1Of","subj":"T26232","obj":"T26231"},{"id":"R18597","pred":"arg1Of","subj":"T26233","obj":"T26232"},{"id":"R18598","pred":"arg1Of","subj":"T26234","obj":"T26228"},{"id":"R18599","pred":"arg1Of","subj":"T26234","obj":"T26233"},{"id":"R18600","pred":"arg1Of","subj":"T26234","obj":"T26235"},{"id":"R18601","pred":"arg2Of","subj":"T26234","obj":"T26237"},{"id":"R18602","pred":"arg2Of","subj":"T26237","obj":"T26235"},{"id":"R18603","pred":"arg1Of","subj":"T26237","obj":"T26236"},{"id":"R18604","pred":"arg1Of","subj":"T26237","obj":"T26238"},{"id":"R18605","pred":"arg1Of","subj":"T26237","obj":"T26244"},{"id":"R18606","pred":"arg1Of","subj":"T26238","obj":"T26242"},{"id":"R18607","pred":"arg2Of","subj":"T26241","obj":"T26238"},{"id":"R18608","pred":"arg1Of","subj":"T26241","obj":"T26239"},{"id":"R18609","pred":"arg1Of","subj":"T26241","obj":"T26240"},{"id":"R18610","pred":"arg2Of","subj":"T26244","obj":"T26242"},{"id":"R18611","pred":"arg1Of","subj":"T26244","obj":"T26243"},{"id":"R18612","pred":"arg2Of","subj":"T26246","obj":"T26244"},{"id":"R18613","pred":"arg1Of","subj":"T26246","obj":"T26245"},{"id":"R18614","pred":"arg1Of","subj":"T26251","obj":"T26247"},{"id":"R18615","pred":"arg1Of","subj":"T26251","obj":"T26248"},{"id":"R18616","pred":"arg1Of","subj":"T26251","obj":"T26249"},{"id":"R18617","pred":"arg1Of","subj":"T26251","obj":"T26250"},{"id":"R18618","pred":"arg2Of","subj":"T26251","obj":"T26252"},{"id":"R18619","pred":"arg1Of","subj":"T26252","obj":"T26253"},{"id":"R18620","pred":"arg1Of","subj":"T26254","obj":"T26255"},{"id":"R18621","pred":"arg2Of","subj":"T26256","obj":"T26255"},{"id":"R18622","pred":"arg2Of","subj":"T26257","obj":"T26253"},{"id":"R18623","pred":"arg1Of","subj":"T26257","obj":"T26254"},{"id":"R18624","pred":"arg1Of","subj":"T26257","obj":"T26256"},{"id":"R18625","pred":"arg1Of","subj":"T26257","obj":"T26258"},{"id":"R18626","pred":"arg1Of","subj":"T26258","obj":"T26264"},{"id":"R18627","pred":"arg2Of","subj":"T26263","obj":"T26258"},{"id":"R18628","pred":"arg1Of","subj":"T26263","obj":"T26259"},{"id":"R18629","pred":"arg1Of","subj":"T26263","obj":"T26260"},{"id":"R18630","pred":"arg1Of","subj":"T26263","obj":"T26261"},{"id":"R18631","pred":"arg1Of","subj":"T26263","obj":"T26262"},{"id":"R18632","pred":"arg1Of","subj":"T26266","obj":"T26267"},{"id":"R18633","pred":"arg2Of","subj":"T26267","obj":"T26264"},{"id":"R18634","pred":"arg1Of","subj":"T26267","obj":"T26265"},{"id":"R18635","pred":"arg1Of","subj":"T26267","obj":"T26269"},{"id":"R18636","pred":"arg2Of","subj":"T26268","obj":"T26267"},{"id":"R18637","pred":"arg2Of","subj":"T26270","obj":"T26269"},{"id":"R18638","pred":"arg1Of","subj":"T26270","obj":"T26271"},{"id":"R18639","pred":"arg2Of","subj":"T26273","obj":"T26271"},{"id":"R18640","pred":"arg1Of","subj":"T26273","obj":"T26272"},{"id":"R18641","pred":"arg3Of","subj":"T26274","obj":"T26271"},{"id":"R18642","pred":"arg1Of","subj":"T26275","obj":"T26276"},{"id":"R18643","pred":"arg2Of","subj":"T26275","obj":"T26277"},{"id":"R18644","pred":"arg2Of","subj":"T26277","obj":"T26276"},{"id":"R18645","pred":"arg1Of","subj":"T26277","obj":"T26278"},{"id":"R18646","pred":"arg1Of","subj":"T26277","obj":"T26289"},{"id":"R18647","pred":"arg1Of","subj":"T26283","obj":"T26279"},{"id":"R18648","pred":"arg1Of","subj":"T26283","obj":"T26280"},{"id":"R18649","pred":"arg1Of","subj":"T26283","obj":"T26281"},{"id":"R18650","pred":"arg1Of","subj":"T26283","obj":"T26282"},{"id":"R18651","pred":"arg1Of","subj":"T26283","obj":"T26284"},{"id":"R18652","pred":"arg2Of","subj":"T26284","obj":"T26278"},{"id":"R18653","pred":"arg2Of","subj":"T26287","obj":"T26284"},{"id":"R18654","pred":"arg1Of","subj":"T26287","obj":"T26285"},{"id":"R18655","pred":"arg1Of","subj":"T26287","obj":"T26286"},{"id":"R18656","pred":"arg1Of","subj":"T26289","obj":"T26288"},{"id":"R18657","pred":"arg1Of","subj":"T26291","obj":"T26290"},{"id":"R18658","pred":"arg1Of","subj":"T26291","obj":"T26292"},{"id":"R18659","pred":"arg2Of","subj":"T26292","obj":"T26289"},{"id":"R18660","pred":"arg1Of","subj":"T26292","obj":"T26293"},{"id":"R18661","pred":"arg2Of","subj":"T26297","obj":"T26296"},{"id":"R18662","pred":"arg3Of","subj":"T26298","obj":"T26296"},{"id":"R18663","pred":"arg1Of","subj":"T26304","obj":"T26301"},{"id":"R18664","pred":"arg1Of","subj":"T26304","obj":"T26302"},{"id":"R18665","pred":"arg1Of","subj":"T26304","obj":"T26303"},{"id":"R18666","pred":"arg1Of","subj":"T26304","obj":"T26305"},{"id":"R18667","pred":"arg2Of","subj":"T26305","obj":"T26293"},{"id":"R18668","pred":"arg1Of","subj":"T26305","obj":"T26294"},{"id":"R18669","pred":"arg1Of","subj":"T26305","obj":"T26295"},{"id":"R18670","pred":"arg1Of","subj":"T26305","obj":"T26296"},{"id":"R18671","pred":"arg1Of","subj":"T26305","obj":"T26299"},{"id":"R18672","pred":"arg1Of","subj":"T26305","obj":"T26300"},{"id":"R18673","pred":"modOf","subj":"T26305","obj":"T26318"},{"id":"R18674","pred":"arg2Of","subj":"T26307","obj":"T26305"},{"id":"R18675","pred":"arg1Of","subj":"T26307","obj":"T26306"},{"id":"R18676","pred":"arg1Of","subj":"T26307","obj":"T26308"},{"id":"R18677","pred":"arg2Of","subj":"T26309","obj":"T26308"},{"id":"R18678","pred":"arg3Of","subj":"T26310","obj":"T26308"},{"id":"R18679","pred":"arg1Of","subj":"T26311","obj":"T26312"},{"id":"R18680","pred":"arg1Of","subj":"T26311","obj":"T26314"},{"id":"R18681","pred":"arg1Of","subj":"T26311","obj":"T26318"},{"id":"R18682","pred":"arg2Of","subj":"T26313","obj":"T26312"},{"id":"R18683","pred":"arg2Of","subj":"T26315","obj":"T26314"},{"id":"R18684","pred":"arg1Of","subj":"T26315","obj":"T26316"},{"id":"R18685","pred":"arg1Of","subj":"T26318","obj":"T26317"},{"id":"R18686","pred":"arg2Of","subj":"T26322","obj":"T26318"},{"id":"R18687","pred":"arg1Of","subj":"T26322","obj":"T26319"},{"id":"R18688","pred":"arg1Of","subj":"T26322","obj":"T26320"},{"id":"R18689","pred":"arg1Of","subj":"T26322","obj":"T26321"},{"id":"R18690","pred":"arg1Of","subj":"T26324","obj":"T26323"},{"id":"R18691","pred":"arg1Of","subj":"T26324","obj":"T26325"},{"id":"R18692","pred":"arg2Of","subj":"T26324","obj":"T26326"},{"id":"R18693","pred":"arg2Of","subj":"T26324","obj":"T26353"},{"id":"R18694","pred":"arg1Of","subj":"T26326","obj":"T26327"},{"id":"R18695","pred":"arg1Of","subj":"T26326","obj":"T26352"},{"id":"R18696","pred":"arg1Of","subj":"T26329","obj":"T26328"},{"id":"R18697","pred":"arg1Of","subj":"T26329","obj":"T26330"},{"id":"R18698","pred":"arg1Of","subj":"T26329","obj":"T26347"},{"id":"R18699","pred":"arg1Of","subj":"T26332","obj":"T26331"},{"id":"R18700","pred":"arg1Of","subj":"T26332","obj":"T26333"},{"id":"R18701","pred":"arg2Of","subj":"T26333","obj":"T26330"},{"id":"R18702","pred":"arg2Of","subj":"T26338","obj":"T26333"},{"id":"R18703","pred":"arg1Of","subj":"T26338","obj":"T26334"},{"id":"R18704","pred":"arg2Of","subj":"T26338","obj":"T26335"},{"id":"R18705","pred":"arg1Of","subj":"T26338","obj":"T26336"},{"id":"R18706","pred":"arg1Of","subj":"T26338","obj":"T26337"},{"id":"R18707","pred":"arg1Of","subj":"T26338","obj":"T26339"},{"id":"R18708","pred":"arg1Of","subj":"T26338","obj":"T26340"},{"id":"R18709","pred":"arg1Of","subj":"T26340","obj":"T26341"},{"id":"R18710","pred":"arg2Of","subj":"T26346","obj":"T26341"},{"id":"R18711","pred":"arg1Of","subj":"T26346","obj":"T26342"},{"id":"R18712","pred":"arg1Of","subj":"T26346","obj":"T26343"},{"id":"R18713","pred":"arg1Of","subj":"T26346","obj":"T26344"},{"id":"R18714","pred":"arg1Of","subj":"T26346","obj":"T26345"},{"id":"R18715","pred":"arg2Of","subj":"T26347","obj":"T26327"},{"id":"R18716","pred":"arg2Of","subj":"T26350","obj":"T26347"},{"id":"R18717","pred":"arg1Of","subj":"T26350","obj":"T26348"},{"id":"R18718","pred":"arg1Of","subj":"T26350","obj":"T26349"},{"id":"R18719","pred":"arg2Of","subj":"T26352","obj":"T26325"},{"id":"R18720","pred":"arg1Of","subj":"T26352","obj":"T26351"},{"id":"R18721","pred":"arg2Of","subj":"T26353","obj":"T26352"},{"id":"R18722","pred":"arg1Of","subj":"T26353","obj":"T26354"},{"id":"R18723","pred":"arg1Of","subj":"T26353","obj":"T26356"},{"id":"R18724","pred":"arg2Of","subj":"T26355","obj":"T26354"},{"id":"R18725","pred":"arg2Of","subj":"T26358","obj":"T26356"},{"id":"R18726","pred":"arg1Of","subj":"T26358","obj":"T26357"},{"id":"R18727","pred":"arg1Of","subj":"T26360","obj":"T26359"},{"id":"R18728","pred":"arg1Of","subj":"T26360","obj":"T26361"},{"id":"R18729","pred":"arg2Of","subj":"T26360","obj":"T26362"},{"id":"R18730","pred":"arg2Of","subj":"T26360","obj":"T26364"},{"id":"R18731","pred":"arg1Of","subj":"T26362","obj":"T26363"},{"id":"R18732","pred":"arg2Of","subj":"T26363","obj":"T26361"},{"id":"R18733","pred":"arg2Of","subj":"T26364","obj":"T26363"},{"id":"R18734","pred":"arg1Of","subj":"T26364","obj":"T26365"},{"id":"R18735","pred":"arg2Of","subj":"T26367","obj":"T26365"},{"id":"R18736","pred":"arg1Of","subj":"T26367","obj":"T26366"},{"id":"R18737","pred":"arg1Of","subj":"T26370","obj":"T26368"},{"id":"R18738","pred":"arg1Of","subj":"T26370","obj":"T26369"},{"id":"R18739","pred":"arg1Of","subj":"T26370","obj":"T26371"},{"id":"R18740","pred":"arg1Of","subj":"T26370","obj":"T26376"},{"id":"R18741","pred":"arg2Of","subj":"T26372","obj":"T26371"},{"id":"R18742","pred":"arg2Of","subj":"T26374","obj":"T26372"},{"id":"R18743","pred":"arg1Of","subj":"T26374","obj":"T26373"},{"id":"R18744","pred":"arg3Of","subj":"T26375","obj":"T26371"},{"id":"R18745","pred":"arg2Of","subj":"T26378","obj":"T26376"},{"id":"R18746","pred":"arg1Of","subj":"T26378","obj":"T26377"},{"id":"R18747","pred":"arg1Of","subj":"T26378","obj":"T26379"},{"id":"R18748","pred":"arg2Of","subj":"T26384","obj":"T26379"},{"id":"R18749","pred":"arg1Of","subj":"T26384","obj":"T26380"},{"id":"R18750","pred":"arg1Of","subj":"T26384","obj":"T26381"},{"id":"R18751","pred":"arg1Of","subj":"T26384","obj":"T26382"},{"id":"R18752","pred":"arg1Of","subj":"T26384","obj":"T26383"},{"id":"R18753","pred":"arg1Of","subj":"T26388","obj":"T26385"},{"id":"R18754","pred":"arg1Of","subj":"T26388","obj":"T26386"},{"id":"R18755","pred":"arg1Of","subj":"T26388","obj":"T26387"},{"id":"R18756","pred":"arg1Of","subj":"T26388","obj":"T26389"},{"id":"R18757","pred":"arg1Of","subj":"T26393","obj":"T26394"},{"id":"R18758","pred":"arg2Of","subj":"T26395","obj":"T26394"},{"id":"R18759","pred":"arg2Of","subj":"T26397","obj":"T26389"},{"id":"R18760","pred":"arg1Of","subj":"T26397","obj":"T26390"},{"id":"R18761","pred":"arg1Of","subj":"T26397","obj":"T26391"},{"id":"R18762","pred":"arg1Of","subj":"T26397","obj":"T26392"},{"id":"R18763","pred":"arg1Of","subj":"T26397","obj":"T26393"},{"id":"R18764","pred":"arg1Of","subj":"T26397","obj":"T26395"},{"id":"R18765","pred":"arg1Of","subj":"T26397","obj":"T26396"},{"id":"R18766","pred":"arg1Of","subj":"T26397","obj":"T26398"},{"id":"R18767","pred":"arg2Of","subj":"T26399","obj":"T26398"},{"id":"R18768","pred":"arg1Of","subj":"T26401","obj":"T26400"},{"id":"R18769","pred":"arg1Of","subj":"T26401","obj":"T26402"},{"id":"R18770","pred":"arg1Of","subj":"T26404","obj":"T26405"},{"id":"R18771","pred":"arg2Of","subj":"T26405","obj":"T26402"},{"id":"R18772","pred":"arg1Of","subj":"T26405","obj":"T26403"},{"id":"R18773","pred":"arg1Of","subj":"T26405","obj":"T26407"},{"id":"R18774","pred":"arg2Of","subj":"T26406","obj":"T26405"},{"id":"R18775","pred":"arg1Of","subj":"T26410","obj":"T26408"},{"id":"R18776","pred":"arg1Of","subj":"T26410","obj":"T26409"},{"id":"R18777","pred":"arg2Of","subj":"T26412","obj":"T26407"},{"id":"R18778","pred":"arg1Of","subj":"T26412","obj":"T26410"},{"id":"R18779","pred":"arg1Of","subj":"T26412","obj":"T26411"},{"id":"R18780","pred":"arg2Of","subj":"T26412","obj":"T26413"},{"id":"R18781","pred":"arg1Of","subj":"T26413","obj":"T26414"},{"id":"R18782","pred":"arg2Of","subj":"T26415","obj":"T26414"},{"id":"R18783","pred":"arg1Of","subj":"T26415","obj":"T26416"},{"id":"R18784","pred":"arg1Of","subj":"T26417","obj":"T26418"},{"id":"R18785","pred":"arg2Of","subj":"T26419","obj":"T26418"},{"id":"R18786","pred":"arg1Of","subj":"T26421","obj":"T26420"},{"id":"R18787","pred":"arg1Of","subj":"T26421","obj":"T26422"},{"id":"R18788","pred":"arg2Of","subj":"T26423","obj":"T26422"},{"id":"R18789","pred":"arg2Of","subj":"T26424","obj":"T26416"},{"id":"R18790","pred":"arg1Of","subj":"T26424","obj":"T26417"},{"id":"R18791","pred":"arg1Of","subj":"T26424","obj":"T26421"},{"id":"R18792","pred":"arg1Of","subj":"T26424","obj":"T26423"},{"id":"R18793","pred":"arg3Of","subj":"T26425","obj":"T26416"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T15768","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T15767","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T15766","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T15765","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T15764","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T15763","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T15762","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T15761","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T15760","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T15759","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T15758","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T15757","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T15756","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T15755","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T15754","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T15753","span":{"begin":0,"end":7},"obj":"Protein"},{"id":"T26146","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26145","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26144","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26143","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26142","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26141","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26140","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26139","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26138","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26137","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26136","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T26135","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26134","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T26133","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T26152","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26151","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26150","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26149","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26148","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26147","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26158","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26157","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26156","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26155","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26154","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26153","span":{"begin":1792,"end":1816},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T26512","span":{"begin":1862,"end":1872},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26511","span":{"begin":1768,"end":1778},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26510","span":{"begin":1343,"end":1353},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26509","span":{"begin":1280,"end":1290},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26508","span":{"begin":1150,"end":1160},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26507","span":{"begin":1750,"end":1758},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26506","span":{"begin":1560,"end":1568},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26505","span":{"begin":1262,"end":1270},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26504","span":{"begin":1132,"end":1140},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26503","span":{"begin":1036,"end":1044},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26502","span":{"begin":917,"end":925},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26501","span":{"begin":872,"end":880},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26500","span":{"begin":1588,"end":1593},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26499","span":{"begin":1093,"end":1098},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26498","span":{"begin":1080,"end":1085},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26497","span":{"begin":1014,"end":1019},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26496","span":{"begin":989,"end":994},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26495","span":{"begin":2019,"end":2026},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26494","span":{"begin":1691,"end":1698},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26493","span":{"begin":1666,"end":1673},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26492","span":{"begin":1503,"end":1510},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26491","span":{"begin":1404,"end":1411},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26490","span":{"begin":847,"end":854},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15962","span":{"begin":497,"end":507},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T15961","span":{"begin":766,"end":774},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T15960","span":{"begin":589,"end":597},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T15959","span":{"begin":488,"end":496},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T15958","span":{"begin":408,"end":416},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T15957","span":{"begin":294,"end":302},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T15956","span":{"begin":101,"end":109},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T15955","span":{"begin":38,"end":46},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T15954","span":{"begin":678,"end":683},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15953","span":{"begin":647,"end":652},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15952","span":{"begin":545,"end":550},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15951","span":{"begin":532,"end":537},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15950","span":{"begin":367,"end":374},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15949","span":{"begin":227,"end":234},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15948","span":{"begin":80,"end":87},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T15947","span":{"begin":0,"end":7},"obj":"http://www.uniprot.org/uniprot/P28033"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"GO-BP","denotations":[{"id":"T26178","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0050397"},{"id":"T26177","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T26176","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T26175","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T26174","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T26173","span":{"begin":1484,"end":1499},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T26172","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26171","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26170","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26169","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26168","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26167","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26166","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_1903770"},{"id":"T26165","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_1903770"},{"id":"T26164","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_1903771"},{"id":"T26163","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_1903771"},{"id":"T26162","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T26161","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T26160","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T26159","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T15771","span":{"begin":324,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0032496"},{"id":"T15770","span":{"begin":385,"end":398},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T15769","span":{"begin":271,"end":284},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"GO-MF","denotations":[{"id":"T26193","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0050397"},{"id":"T26192","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T26191","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T26190","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T26189","span":{"begin":1862,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T26188","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26187","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26181","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T26180","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T26179","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T26186","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26185","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26184","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26183","span":{"begin":1374,"end":1398},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26182","span":{"begin":1915,"end":1939},"obj":"http://purl.obolibrary.org/obo/GO_0004336"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"GO-CC","denotations":[{"id":"T15772","span":{"begin":315,"end":320},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"sentences","denotations":[{"id":"T26094","span":{"begin":2144,"end":2277},"obj":"Sentence"},{"id":"T26093","span":{"begin":2053,"end":2143},"obj":"Sentence"},{"id":"T26092","span":{"begin":1941,"end":2052},"obj":"Sentence"},{"id":"T26091","span":{"begin":1862,"end":1940},"obj":"Sentence"},{"id":"T26090","span":{"begin":1588,"end":1861},"obj":"Sentence"},{"id":"T26089","span":{"begin":1217,"end":1587},"obj":"Sentence"},{"id":"T26088","span":{"begin":1029,"end":1216},"obj":"Sentence"},{"id":"T26087","span":{"begin":909,"end":1028},"obj":"Sentence"},{"id":"T26086","span":{"begin":847,"end":908},"obj":"Sentence"},{"id":"T15713","span":{"begin":560,"end":802},"obj":"Sentence"},{"id":"T15712","span":{"begin":341,"end":559},"obj":"Sentence"},{"id":"T15711","span":{"begin":74,"end":340},"obj":"Sentence"},{"id":"T15710","span":{"begin":0,"end":73},"obj":"Sentence"},{"id":"T219","span":{"begin":0,"end":73},"obj":"Sentence"},{"id":"T220","span":{"begin":74,"end":340},"obj":"Sentence"},{"id":"T221","span":{"begin":341,"end":559},"obj":"Sentence"},{"id":"T222","span":{"begin":560,"end":802},"obj":"Sentence"},{"id":"T223","span":{"begin":803,"end":908},"obj":"Sentence"},{"id":"T224","span":{"begin":909,"end":1028},"obj":"Sentence"},{"id":"T225","span":{"begin":1029,"end":1216},"obj":"Sentence"},{"id":"T226","span":{"begin":1217,"end":1587},"obj":"Sentence"},{"id":"T227","span":{"begin":1588,"end":1861},"obj":"Sentence"},{"id":"T228","span":{"begin":1862,"end":1940},"obj":"Sentence"},{"id":"T229","span":{"begin":1941,"end":2052},"obj":"Sentence"},{"id":"T230","span":{"begin":2053,"end":2143},"obj":"Sentence"},{"id":"T231","span":{"begin":2144,"end":2277},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"simple1","denotations":[{"id":"T26219","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26218","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26217","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26216","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26215","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26214","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26213","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26212","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26211","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26210","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26209","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26208","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26207","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26206","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26205","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26204","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26203","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26202","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26201","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26200","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26199","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26198","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26197","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T26196","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26195","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T26194","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T15788","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T15787","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T15786","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T15785","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T15784","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T15783","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T15782","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T15781","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T15780","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T15779","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T15778","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T15777","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T15776","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T15775","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T15774","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T15773","span":{"begin":0,"end":7},"obj":"Protein"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T26464","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26463","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26462","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26461","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26460","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26459","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26458","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26457","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26456","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26455","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T26454","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26453","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T26452","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T15934","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T15933","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T15932","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T15931","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T15930","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T15929","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T15928","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T15927","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T15926","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T15925","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T15924","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T15923","span":{"begin":0,"end":7},"obj":"Protein"},{"id":"T15946","span":{"begin":574,"end":588},"obj":"Positive_regulation"},{"id":"T15945","span":{"begin":665,"end":674},"obj":"Negative_regulation"},{"id":"T15944","span":{"begin":754,"end":765},"obj":"Positive_regulation"},{"id":"T15943","span":{"begin":741,"end":749},"obj":"Positive_regulation"},{"id":"T15942","span":{"begin":634,"end":643},"obj":"Positive_regulation"},{"id":"T15941","span":{"begin":385,"end":398},"obj":"Transcription"},{"id":"T15940","span":{"begin":88,"end":93},"obj":"Binding"},{"id":"T15939","span":{"begin":271,"end":284},"obj":"Transcription"},{"id":"T15938","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T15937","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T15936","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T15935","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T26489","span":{"begin":2088,"end":2095},"obj":"Gene_expression"},{"id":"T26488","span":{"begin":1990,"end":2000},"obj":"Gene_expression"},{"id":"T26487","span":{"begin":1607,"end":1621},"obj":"Gene_expression"},{"id":"T26486","span":{"begin":1831,"end":1841},"obj":"Positive_regulation"},{"id":"T26485","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26484","span":{"begin":1484,"end":1499},"obj":"Phosphorylation"},{"id":"T26483","span":{"begin":1535,"end":1544},"obj":"Regulation"},{"id":"T26482","span":{"begin":1545,"end":1559},"obj":"Positive_regulation"},{"id":"T26481","span":{"begin":1228,"end":1242},"obj":"Gene_expression"},{"id":"T26480","span":{"begin":1107,"end":1117},"obj":"Gene_expression"},{"id":"T26479","span":{"begin":961,"end":970},"obj":"Positive_regulation"},{"id":"T26478","span":{"begin":858,"end":867},"obj":"Positive_regulation"},{"id":"T26477","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26476","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26475","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26474","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26473","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26472","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26471","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26470","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26469","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26468","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26467","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26466","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26465","span":{"begin":1404,"end":1411},"obj":"Protein"}],"relations":[{"id":"R11743","pred":"themeOf","subj":"T15925","obj":"T15940"},{"id":"R11744","pred":"themeOf","subj":"T15926","obj":"T15940"},{"id":"R11745","pred":"themeOf","subj":"T15928","obj":"T15939"},{"id":"R11746","pred":"themeOf","subj":"T15932","obj":"T15941"},{"id":"R11747","pred":"themeOf","subj":"T15934","obj":"T15942"},{"id":"R11748","pred":"themeOf","subj":"T15934","obj":"T15945"},{"id":"R11749","pred":"themeOf","subj":"T15934","obj":"T15946"},{"id":"R11750","pred":"themeOf","subj":"T15935","obj":"T15942"},{"id":"R11751","pred":"causeOf","subj":"T15937","obj":"T15943"},{"id":"R11752","pred":"causeOf","subj":"T15937","obj":"T15943"},{"id":"R11753","pred":"themeOf","subj":"T15938","obj":"T15943"},{"id":"R11754","pred":"themeOf","subj":"T15938","obj":"T15944"},{"id":"R11755","pred":"themeOf","subj":"T15944","obj":"T15943"},{"id":"R11756","pred":"themeOf","subj":"T15946","obj":"T15942"},{"id":"R11757","pred":"themeOf","subj":"T15946","obj":"T15945"},{"id":"R18794","pred":"causeOf","subj":"T26452","obj":"T26478"},{"id":"R18795","pred":"themeOf","subj":"T26453","obj":"T26478"},{"id":"R18796","pred":"themeOf","subj":"T26454","obj":"T26479"},{"id":"R18797","pred":"themeOf","subj":"T26455","obj":"T26479"},{"id":"R18798","pred":"themeOf","subj":"T26460","obj":"T26480"},{"id":"R18799","pred":"themeOf","subj":"T26461","obj":"T26481"},{"id":"R18801","pred":"themeOf","subj":"T26467","obj":"T26483"},{"id":"R18808","pred":"themeOf","subj":"T26482","obj":"T26483"},{"id":"R18800","pred":"themeOf","subj":"T26466","obj":"T26484"},{"id":"R18802","pred":"themeOf","subj":"T26467","obj":"T26482"},{"id":"R18803","pred":"themeOf","subj":"T26468","obj":"T26486"},{"id":"R18804","pred":"themeOf","subj":"T26468","obj":"T26487"},{"id":"R18805","pred":"themeOf","subj":"T26469","obj":"T26485"},{"id":"R18806","pred":"themeOf","subj":"T26475","obj":"T26488"},{"id":"R18807","pred":"themeOf","subj":"T26477","obj":"T26489"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T26612","span":{"begin":2088,"end":2095},"obj":"Gene_expression"},{"id":"T26611","span":{"begin":1990,"end":2000},"obj":"Gene_expression"},{"id":"T26610","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26609","span":{"begin":1484,"end":1499},"obj":"Phosphorylation"},{"id":"T26608","span":{"begin":1228,"end":1242},"obj":"Gene_expression"},{"id":"T26607","span":{"begin":1107,"end":1117},"obj":"Gene_expression"},{"id":"T26606","span":{"begin":961,"end":970},"obj":"Positive_regulation"},{"id":"T26605","span":{"begin":858,"end":867},"obj":"Positive_regulation"},{"id":"T26604","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26603","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26602","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26601","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26600","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26599","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26598","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26597","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26596","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26595","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26594","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26593","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26592","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26591","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26590","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26589","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26588","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26587","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26586","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26585","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26584","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26583","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26582","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T26581","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26580","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T16025","span":{"begin":741,"end":749},"obj":"Positive_regulation"},{"id":"T16024","span":{"begin":634,"end":643},"obj":"Positive_regulation"},{"id":"T16023","span":{"begin":665,"end":674},"obj":"Negative_regulation"},{"id":"T16022","span":{"begin":754,"end":765},"obj":"Positive_regulation"},{"id":"T16021","span":{"begin":242,"end":251},"obj":"Positive_regulation"},{"id":"T16020","span":{"begin":88,"end":93},"obj":"Binding"},{"id":"T16019","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T16018","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T16017","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T16016","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T16015","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T16014","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T16013","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T16012","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T16011","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T16010","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T16009","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T16008","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T16007","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T16006","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T16005","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T16004","span":{"begin":0,"end":7},"obj":"Protein"},{"id":"T26579","span":{"begin":847,"end":854},"obj":"Protein"}],"relations":[{"id":"R11772","pred":"themeOf","subj":"T16006","obj":"T16020"},{"id":"R11773","pred":"themeOf","subj":"T16007","obj":"T16020"},{"id":"R11774","pred":"themeOf","subj":"T16008","obj":"T16021"},{"id":"R11775","pred":"themeOf","subj":"T16016","obj":"T16024"},{"id":"R11776","pred":"themeOf","subj":"T16017","obj":"T16023"},{"id":"R11777","pred":"causeOf","subj":"T16018","obj":"T16025"},{"id":"R11778","pred":"themeOf","subj":"T16019","obj":"T16025"},{"id":"R11779","pred":"themeOf","subj":"T16019","obj":"T16022"},{"id":"R18832","pred":"themeOf","subj":"T26579","obj":"T26605"},{"id":"R18833","pred":"themeOf","subj":"T26581","obj":"T26606"},{"id":"R18834","pred":"themeOf","subj":"T26582","obj":"T26606"},{"id":"R18835","pred":"themeOf","subj":"T26587","obj":"T26607"},{"id":"R18836","pred":"themeOf","subj":"T26588","obj":"T26608"},{"id":"R18837","pred":"themeOf","subj":"T26593","obj":"T26609"},{"id":"R18838","pred":"themeOf","subj":"T26596","obj":"T26610"},{"id":"R18839","pred":"themeOf","subj":"T26602","obj":"T26611"},{"id":"R18840","pred":"themeOf","subj":"T26604","obj":"T26612"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"DLUT931","denotations":[{"id":"T26514","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T26513","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T15985","span":{"begin":754,"end":765},"obj":"Positive_regulation"},{"id":"T15984","span":{"begin":665,"end":674},"obj":"Negative_regulation"},{"id":"T15983","span":{"begin":634,"end":643},"obj":"Positive_regulation"},{"id":"T15982","span":{"begin":242,"end":251},"obj":"Positive_regulation"},{"id":"T15981","span":{"begin":271,"end":284},"obj":"Transcription"},{"id":"T15980","span":{"begin":88,"end":93},"obj":"Binding"},{"id":"T15979","span":{"begin":17,"end":30},"obj":"Positive_regulation"},{"id":"T15978","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T15977","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T15976","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T15975","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T15974","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T15973","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T15972","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T15971","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T15970","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T15969","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T15968","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T15967","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T15966","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T15965","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T15964","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T15963","span":{"begin":0,"end":7},"obj":"Protein"},{"id":"T26527","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26526","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26525","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26524","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26523","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26522","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26521","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26520","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26519","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26518","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26517","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26516","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T26515","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26548","span":{"begin":2088,"end":2095},"obj":"Gene_expression"},{"id":"T26547","span":{"begin":2070,"end":2082},"obj":"Gene_expression"},{"id":"T26546","span":{"begin":1990,"end":2000},"obj":"Gene_expression"},{"id":"T26545","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26544","span":{"begin":1535,"end":1544},"obj":"Regulation"},{"id":"T26543","span":{"begin":1545,"end":1559},"obj":"Positive_regulation"},{"id":"T26542","span":{"begin":1484,"end":1499},"obj":"Phosphorylation"},{"id":"T26541","span":{"begin":1228,"end":1242},"obj":"Gene_expression"},{"id":"T26540","span":{"begin":1107,"end":1117},"obj":"Gene_expression"},{"id":"T26539","span":{"begin":961,"end":970},"obj":"Positive_regulation"},{"id":"T26538","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26537","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26536","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26535","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26534","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26533","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26532","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26531","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26530","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26529","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26528","span":{"begin":1560,"end":1568},"obj":"Protein"}],"relations":[{"id":"R11758","pred":"themeOf","subj":"T15964","obj":"T15979"},{"id":"R11759","pred":"themeOf","subj":"T15965","obj":"T15980"},{"id":"R11760","pred":"themeOf","subj":"T15966","obj":"T15980"},{"id":"R11761","pred":"causeOf","subj":"T15967","obj":"T15982"},{"id":"R11762","pred":"themeOf","subj":"T15968","obj":"T15981"},{"id":"R11763","pred":"themeOf","subj":"T15974","obj":"T15983"},{"id":"R11764","pred":"themeOf","subj":"T15974","obj":"T15984"},{"id":"R11765","pred":"themeOf","subj":"T15978","obj":"T15985"},{"id":"R11766","pred":"themeOf","subj":"T15981","obj":"T15982"},{"id":"R18809","pred":"themeOf","subj":"T26515","obj":"T26539"},{"id":"R18810","pred":"themeOf","subj":"T26521","obj":"T26540"},{"id":"R18811","pred":"themeOf","subj":"T26522","obj":"T26541"},{"id":"R18812","pred":"themeOf","subj":"T26523","obj":"T26541"},{"id":"R18813","pred":"themeOf","subj":"T26527","obj":"T26542"},{"id":"R18814","pred":"themeOf","subj":"T26528","obj":"T26543"},{"id":"R18815","pred":"themeOf","subj":"T26529","obj":"T26545"},{"id":"R18816","pred":"themeOf","subj":"T26530","obj":"T26545"},{"id":"R18817","pred":"themeOf","subj":"T26531","obj":"T26545"},{"id":"R18818","pred":"themeOf","subj":"T26532","obj":"T26545"},{"id":"R18819","pred":"themeOf","subj":"T26533","obj":"T26545"},{"id":"R18820","pred":"themeOf","subj":"T26536","obj":"T26546"},{"id":"R18821","pred":"themeOf","subj":"T26537","obj":"T26547"},{"id":"R18822","pred":"themeOf","subj":"T26537","obj":"T26548"},{"id":"R18823","pred":"themeOf","subj":"T26538","obj":"T26548"},{"id":"R18824","pred":"causeOf","subj":"T26542","obj":"T26544"},{"id":"R18825","pred":"themeOf","subj":"T26543","obj":"T26544"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T26746","span":{"begin":974,"end":1002},"obj":"Positive_regulation"},{"id":"T26745","span":{"begin":1404,"end":1451},"obj":"Gene_expression"},{"id":"T26744","span":{"begin":1102,"end":1203},"obj":"Gene_expression"},{"id":"T26743","span":{"begin":1545,"end":1586},"obj":"Positive_regulation"},{"id":"T26742","span":{"begin":1484,"end":1586},"obj":"Regulation"},{"id":"T26741","span":{"begin":1736,"end":1787},"obj":"Protein"},{"id":"T26740","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26739","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26738","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26737","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T26736","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26735","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26734","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26733","span":{"begin":1597,"end":1600},"obj":"Protein"},{"id":"T26732","span":{"begin":1102,"end":1203},"obj":"Protein"},{"id":"T26731","span":{"begin":1656,"end":1673},"obj":"Protein"},{"id":"T26730","span":{"begin":1118,"end":1169},"obj":"Protein"},{"id":"T26729","span":{"begin":1248,"end":1299},"obj":"Protein"},{"id":"T26728","span":{"begin":872,"end":898},"obj":"Entity"},{"id":"T26727","span":{"begin":913,"end":943},"obj":"Entity"},{"id":"T26726","span":{"begin":1023,"end":1027},"obj":"Protein"},{"id":"T26725","span":{"begin":1217,"end":1222},"obj":"Entity"},{"id":"T26724","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26723","span":{"begin":1253,"end":1257},"obj":"Protein"},{"id":"T26722","span":{"begin":1029,"end":1067},"obj":"Protein"},{"id":"T26721","span":{"begin":974,"end":988},"obj":"Entity"},{"id":"T26720","span":{"begin":2057,"end":2082},"obj":"Protein"},{"id":"T26719","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26718","span":{"begin":1560,"end":1577},"obj":"Entity"},{"id":"T26717","span":{"begin":1847,"end":1850},"obj":"Entity"},{"id":"T26716","span":{"begin":1500,"end":1510},"obj":"Protein"},{"id":"T26715","span":{"begin":1855,"end":1860},"obj":"Protein"},{"id":"T26714","span":{"begin":1404,"end":1451},"obj":"Protein"},{"id":"T26713","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26712","span":{"begin":1050,"end":1058},"obj":"Entity"},{"id":"T26711","span":{"begin":1741,"end":1745},"obj":"Protein"},{"id":"T26710","span":{"begin":2053,"end":2087},"obj":"Protein"},{"id":"T26709","span":{"begin":1514,"end":1523},"obj":"Entity"},{"id":"T26708","span":{"begin":1123,"end":1127},"obj":"Protein"},{"id":"T26707","span":{"begin":1197,"end":1203},"obj":"Entity"},{"id":"T26706","span":{"begin":1545,"end":1559},"obj":"Entity"},{"id":"T26705","span":{"begin":1588,"end":1594},"obj":"Protein"},{"id":"T26704","span":{"begin":1093,"end":1099},"obj":"Protein"},{"id":"T26703","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T26702","span":{"begin":1456,"end":1478},"obj":"Entity"},{"id":"T26701","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26700","span":{"begin":1437,"end":1441},"obj":"Protein"},{"id":"T26699","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26698","span":{"begin":2004,"end":2051},"obj":"Protein"},{"id":"T26697","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26696","span":{"begin":974,"end":1002},"obj":"Protein"},{"id":"T26695","span":{"begin":1691,"end":1724},"obj":"Protein"},{"id":"T26694","span":{"begin":1464,"end":1472},"obj":"Entity"},{"id":"T26693","span":{"begin":2262,"end":2275},"obj":"Protein"},{"id":"T16105","span":{"begin":74,"end":180},"obj":"Binding"},{"id":"T16104","span":{"begin":574,"end":615},"obj":"Positive_regulation"},{"id":"T16103","span":{"begin":375,"end":425},"obj":"Regulation"},{"id":"T16102","span":{"begin":0,"end":73},"obj":"Positive_regulation"},{"id":"T16101","span":{"begin":554,"end":558},"obj":"Protein"},{"id":"T16100","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T16099","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T16098","span":{"begin":404,"end":425},"obj":"Protein"},{"id":"T16097","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T16096","span":{"begin":315,"end":320},"obj":"Entity"},{"id":"T16095","span":{"begin":784,"end":792},"obj":"Entity"},{"id":"T16094","span":{"begin":0,"end":7},"obj":"Protein"},{"id":"T16093","span":{"begin":56,"end":64},"obj":"Entity"},{"id":"T16092","span":{"begin":426,"end":434},"obj":"Entity"},{"id":"T16091","span":{"begin":472,"end":476},"obj":"Protein"},{"id":"T16090","span":{"begin":656,"end":660},"obj":"Protein"},{"id":"T16089","span":{"begin":693,"end":696},"obj":"Protein"},{"id":"T16088","span":{"begin":290,"end":311},"obj":"Entity"},{"id":"T16087","span":{"begin":574,"end":588},"obj":"Entity"},{"id":"T16086","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T16085","span":{"begin":545,"end":551},"obj":"Protein"},{"id":"T16084","span":{"begin":714,"end":737},"obj":"Protein"},{"id":"T16083","span":{"begin":687,"end":691},"obj":"Protein"},{"id":"T16082","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T16081","span":{"begin":754,"end":801},"obj":"Protein"},{"id":"T16080","span":{"begin":353,"end":374},"obj":"Protein"},{"id":"T16079","span":{"begin":754,"end":765},"obj":"Entity"},{"id":"T16078","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T16077","span":{"begin":589,"end":606},"obj":"Entity"},{"id":"T16076","span":{"begin":678,"end":684},"obj":"Protein"},{"id":"T16075","span":{"begin":94,"end":127},"obj":"Entity"},{"id":"T16074","span":{"begin":336,"end":339},"obj":"Entity"},{"id":"T16073","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T16072","span":{"begin":488,"end":516},"obj":"Protein"}],"relations":[{"id":"R11800","pred":"partOf","subj":"T16075","obj":"T16078"},{"id":"R11801","pred":"themeOf","subj":"T16075","obj":"T16105"},{"id":"R11802","pred":"partOf","subj":"T16077","obj":"T16086"},{"id":"R11803","pred":"causeOf","subj":"T16087","obj":"T16104"},{"id":"R11804","pred":"partOf","subj":"T16088","obj":"T16082"},{"id":"R11805","pred":"causeOf","subj":"T16094","obj":"T16102"},{"id":"R11806","pred":"themeOf","subj":"T16097","obj":"T16105"},{"id":"R18877","pred":"themeOf","subj":"T26696","obj":"T26746"},{"id":"R18878","pred":"themeOf","subj":"T26700","obj":"T26745"},{"id":"R18879","pred":"causeOf","subj":"T26706","obj":"T26743"},{"id":"R18880","pred":"partOf","subj":"T26718","obj":"T26713"},{"id":"R18881","pred":"causeOf","subj":"T26721","obj":"T26746"},{"id":"R18882","pred":"partOf","subj":"T26727","obj":"T26699"},{"id":"R18883","pred":"partOf","subj":"T26728","obj":"T26737"},{"id":"R18884","pred":"themeOf","subj":"T26730","obj":"T26744"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T26652","span":{"begin":2088,"end":2095},"obj":"Gene_expression"},{"id":"T26651","span":{"begin":2088,"end":2095},"obj":"Gene_expression"},{"id":"T26650","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26649","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26648","span":{"begin":1977,"end":1985},"obj":"Regulation"},{"id":"T26647","span":{"begin":1990,"end":2000},"obj":"Gene_expression"},{"id":"T26646","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26645","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26644","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26643","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26642","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26641","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26640","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26639","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26638","span":{"begin":1768,"end":1778},"obj":"Protein"},{"id":"T26637","span":{"begin":1740,"end":1767},"obj":"Protein"},{"id":"T26636","span":{"begin":1691,"end":1698},"obj":"Protein"},{"id":"T26635","span":{"begin":1484,"end":1499},"obj":"Phosphorylation"},{"id":"T26634","span":{"begin":1514,"end":1520},"obj":"Entity"},{"id":"T26633","span":{"begin":1560,"end":1577},"obj":"Protein"},{"id":"T26632","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26631","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26630","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26629","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26628","span":{"begin":1280,"end":1290},"obj":"Protein"},{"id":"T26627","span":{"begin":1252,"end":1279},"obj":"Protein"},{"id":"T26626","span":{"begin":1107,"end":1117},"obj":"Gene_expression"},{"id":"T26625","span":{"begin":1107,"end":1117},"obj":"Gene_expression"},{"id":"T26624","span":{"begin":1150,"end":1160},"obj":"Protein"},{"id":"T26623","span":{"begin":1122,"end":1149},"obj":"Protein"},{"id":"T26622","span":{"begin":1093,"end":1106},"obj":"Protein"},{"id":"T26621","span":{"begin":961,"end":970},"obj":"Positive_regulation"},{"id":"T26620","span":{"begin":961,"end":970},"obj":"Positive_regulation"},{"id":"T26619","span":{"begin":961,"end":970},"obj":"Positive_regulation"},{"id":"T26618","span":{"begin":1014,"end":1027},"obj":"Protein"},{"id":"T26617","span":{"begin":989,"end":1002},"obj":"Protein"},{"id":"T26616","span":{"begin":917,"end":943},"obj":"Protein"},{"id":"T26615","span":{"begin":858,"end":867},"obj":"Positive_regulation"},{"id":"T26614","span":{"begin":872,"end":898},"obj":"Protein"},{"id":"T26613","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T16048","span":{"begin":741,"end":749},"obj":"Positive_regulation"},{"id":"T16047","span":{"begin":665,"end":674},"obj":"Negative_regulation"},{"id":"T16046","span":{"begin":634,"end":643},"obj":"Positive_regulation"},{"id":"T16045","span":{"begin":766,"end":792},"obj":"Protein"},{"id":"T16044","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T16043","span":{"begin":647,"end":660},"obj":"Protein"},{"id":"T16042","span":{"begin":589,"end":606},"obj":"Protein"},{"id":"T16041","span":{"begin":385,"end":398},"obj":"Transcription"},{"id":"T16040","span":{"begin":545,"end":558},"obj":"Protein"},{"id":"T16039","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T16038","span":{"begin":488,"end":507},"obj":"Protein"},{"id":"T16037","span":{"begin":408,"end":434},"obj":"Protein"},{"id":"T16036","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T16035","span":{"begin":271,"end":284},"obj":"Transcription"},{"id":"T16034","span":{"begin":88,"end":93},"obj":"Binding"},{"id":"T16033","span":{"begin":88,"end":93},"obj":"Binding"},{"id":"T16032","span":{"begin":294,"end":311},"obj":"Protein"},{"id":"T16031","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T16030","span":{"begin":101,"end":127},"obj":"Protein"},{"id":"T16029","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T16028","span":{"begin":17,"end":30},"obj":"Positive_regulation"},{"id":"T16027","span":{"begin":31,"end":64},"obj":"Protein"},{"id":"T16026","span":{"begin":0,"end":7},"obj":"Protein"}],"relations":[{"id":"R11780","pred":"themeOf","subj":"T16027","obj":"T16028"},{"id":"R11781","pred":"themeOf","subj":"T16029","obj":"T16033"},{"id":"R11782","pred":"themeOf","subj":"T16029","obj":"T16034"},{"id":"R11783","pred":"themeOf","subj":"T16030","obj":"T16034"},{"id":"R11784","pred":"themeOf","subj":"T16032","obj":"T16035"},{"id":"R11785","pred":"themeOf","subj":"T16037","obj":"T16041"},{"id":"R11786","pred":"themeOf","subj":"T16042","obj":"T16047"},{"id":"R11787","pred":"themeOf","subj":"T16043","obj":"T16046"},{"id":"R11788","pred":"causeOf","subj":"T16044","obj":"T16048"},{"id":"R11789","pred":"themeOf","subj":"T16045","obj":"T16048"},{"id":"R18841","pred":"causeOf","subj":"T26613","obj":"T26615"},{"id":"R18842","pred":"themeOf","subj":"T26614","obj":"T26615"},{"id":"R18843","pred":"themeOf","subj":"T26616","obj":"T26619"},{"id":"R18844","pred":"themeOf","subj":"T26617","obj":"T26620"},{"id":"R18845","pred":"themeOf","subj":"T26618","obj":"T26621"},{"id":"R18846","pred":"themeOf","subj":"T26622","obj":"T26625"},{"id":"R18847","pred":"themeOf","subj":"T26624","obj":"T26626"},{"id":"R18848","pred":"themeOf","subj":"T26632","obj":"T26635"},{"id":"R18849","pred":"partOf","subj":"T26632","obj":"T26634"},{"id":"R18850","pred":"Site","subj":"T26634","obj":"T26635"},{"id":"R18851","pred":"themeOf","subj":"T26636","obj":"T26640"},{"id":"R18852","pred":"themeOf","subj":"T26637","obj":"T26641"},{"id":"R18853","pred":"themeOf","subj":"T26638","obj":"T26642"},{"id":"R18854","pred":"themeOf","subj":"T26639","obj":"T26643"},{"id":"R18855","pred":"themeOf","subj":"T26646","obj":"T26647"},{"id":"R18856","pred":"themeOf","subj":"T26647","obj":"T26648"},{"id":"R18857","pred":"themeOf","subj":"T26649","obj":"T26651"},{"id":"R18858","pred":"themeOf","subj":"T26650","obj":"T26652"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"testone","denotations":[{"id":"T26026","span":{"begin":2088,"end":2095},"obj":"Gene_expression"},{"id":"T26025","span":{"begin":1990,"end":2000},"obj":"Gene_expression"},{"id":"T26024","span":{"begin":1484,"end":1499},"obj":"Phosphorylation"},{"id":"T26023","span":{"begin":1228,"end":1242},"obj":"Gene_expression"},{"id":"T26022","span":{"begin":1107,"end":1117},"obj":"Gene_expression"},{"id":"T26021","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26020","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26019","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26018","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26017","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26016","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26015","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26014","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26013","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26012","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26011","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26010","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26009","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26008","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26007","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26006","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26005","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26004","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26003","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26002","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26001","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26000","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T25999","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T25998","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T25997","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T25996","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T15670","span":{"begin":665,"end":674},"obj":"Negative_regulation"},{"id":"T15669","span":{"begin":451,"end":463},"obj":"Gene_expression"},{"id":"T15668","span":{"begin":426,"end":434},"obj":"Entity"},{"id":"T15667","span":{"begin":385,"end":398},"obj":"Transcription"},{"id":"T15666","span":{"begin":119,"end":127},"obj":"Entity"},{"id":"T15665","span":{"begin":110,"end":118},"obj":"Entity"},{"id":"T15664","span":{"begin":88,"end":93},"obj":"Binding"},{"id":"T15663","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T15662","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T15661","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T15660","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T15659","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T15658","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T15657","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T15656","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T15655","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T15654","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T15653","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T15652","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T15651","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T15650","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T15649","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T15648","span":{"begin":0,"end":7},"obj":"Protein"}],"relations":[{"id":"R11580","pred":"themeOf","subj":"T15650","obj":"T15664"},{"id":"R11581","pred":"themeOf","subj":"T15654","obj":"T15667"},{"id":"R11582","pred":"themeOf","subj":"T15656","obj":"T15669"},{"id":"R11583","pred":"themeOf","subj":"T15666","obj":"T15664"},{"id":"R11584","pred":"themeOf","subj":"T15668","obj":"T15667"},{"id":"R18557","pred":"themeOf","subj":"T26004","obj":"T26022"},{"id":"R18558","pred":"themeOf","subj":"T26005","obj":"T26023"},{"id":"R18559","pred":"themeOf","subj":"T26006","obj":"T26023"},{"id":"R18560","pred":"themeOf","subj":"T26007","obj":"T26023"},{"id":"R18561","pred":"themeOf","subj":"T26008","obj":"T26023"},{"id":"R18562","pred":"themeOf","subj":"T26010","obj":"T26024"},{"id":"R18563","pred":"themeOf","subj":"T26019","obj":"T26025"},{"id":"R18564","pred":"themeOf","subj":"T26021","obj":"T26026"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}

{"project":"test3","denotations":[{"id":"T26085","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26084","span":{"begin":2088,"end":2095},"obj":"Gene_expression"},{"id":"T26083","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26082","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26081","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26080","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26079","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26078","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26077","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26076","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26075","span":{"begin":1627,"end":1637},"obj":"Gene_expression"},{"id":"T26074","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26073","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26072","span":{"begin":1549,"end":1559},"obj":"Positive_regulation"},{"id":"T26071","span":{"begin":1535,"end":1544},"obj":"Regulation"},{"id":"T26070","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26069","span":{"begin":1484,"end":1499},"obj":"Phosphorylation"},{"id":"T26068","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26067","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26066","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26065","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26064","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26063","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26062","span":{"begin":1107,"end":1117},"obj":"Gene_expression"},{"id":"T26061","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26060","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26059","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26058","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26057","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T26056","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26055","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T26054","span":{"begin":858,"end":867},"obj":"Positive_regulation"},{"id":"T26053","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T26052","span":{"begin":2135,"end":2142},"obj":"Protein"},{"id":"T26051","span":{"begin":2057,"end":2069},"obj":"Protein"},{"id":"T26050","span":{"begin":2019,"end":2051},"obj":"Protein"},{"id":"T26049","span":{"begin":1915,"end":1930},"obj":"Protein"},{"id":"T26048","span":{"begin":1862,"end":1872},"obj":"Protein"},{"id":"T26047","span":{"begin":1792,"end":1816},"obj":"Protein"},{"id":"T26046","span":{"begin":1750,"end":1787},"obj":"Protein"},{"id":"T26045","span":{"begin":1691,"end":1723},"obj":"Protein"},{"id":"T26044","span":{"begin":1666,"end":1673},"obj":"Protein"},{"id":"T26043","span":{"begin":1588,"end":1593},"obj":"Protein"},{"id":"T26042","span":{"begin":1560,"end":1568},"obj":"Protein"},{"id":"T26041","span":{"begin":1503,"end":1510},"obj":"Protein"},{"id":"T26040","span":{"begin":1404,"end":1411},"obj":"Protein"},{"id":"T26039","span":{"begin":1374,"end":1389},"obj":"Protein"},{"id":"T26038","span":{"begin":1343,"end":1353},"obj":"Protein"},{"id":"T26037","span":{"begin":1304,"end":1328},"obj":"Protein"},{"id":"T26036","span":{"begin":1262,"end":1299},"obj":"Protein"},{"id":"T26035","span":{"begin":1132,"end":1169},"obj":"Protein"},{"id":"T26034","span":{"begin":1093,"end":1098},"obj":"Protein"},{"id":"T26033","span":{"begin":1080,"end":1085},"obj":"Protein"},{"id":"T26032","span":{"begin":1036,"end":1044},"obj":"Protein"},{"id":"T26031","span":{"begin":1014,"end":1019},"obj":"Protein"},{"id":"T26030","span":{"begin":989,"end":994},"obj":"Protein"},{"id":"T26029","span":{"begin":917,"end":925},"obj":"Protein"},{"id":"T26028","span":{"begin":872,"end":880},"obj":"Protein"},{"id":"T26027","span":{"begin":847,"end":854},"obj":"Protein"},{"id":"T15693","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T15692","span":{"begin":119,"end":127},"obj":"Entity"},{"id":"T15691","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T15690","span":{"begin":88,"end":93},"obj":"Binding"},{"id":"T15689","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T15688","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T15687","span":{"begin":0,"end":7},"obj":"Protein"},{"id":"T15686","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T15685","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T15684","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T15683","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T15682","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T15681","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T15680","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T15679","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T15678","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T15677","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T15676","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T15675","span":{"begin":227,"end":234},"obj":"Protein"},{"id":"T15674","span":{"begin":101,"end":109},"obj":"Protein"},{"id":"T15673","span":{"begin":80,"end":87},"obj":"Protein"},{"id":"T15672","span":{"begin":38,"end":46},"obj":"Protein"},{"id":"T15671","span":{"begin":0,"end":7},"obj":"Protein"},{"id":"T15709","span":{"begin":766,"end":774},"obj":"Protein"},{"id":"T15708","span":{"begin":758,"end":765},"obj":"Positive_regulation"},{"id":"T15707","span":{"begin":730,"end":737},"obj":"Protein"},{"id":"T15706","span":{"begin":678,"end":683},"obj":"Protein"},{"id":"T15705","span":{"begin":647,"end":652},"obj":"Protein"},{"id":"T15704","span":{"begin":589,"end":597},"obj":"Protein"},{"id":"T15703","span":{"begin":578,"end":588},"obj":"Positive_regulation"},{"id":"T15702","span":{"begin":545,"end":550},"obj":"Protein"},{"id":"T15701","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T15700","span":{"begin":488,"end":516},"obj":"Protein"},{"id":"T15699","span":{"begin":408,"end":416},"obj":"Protein"},{"id":"T15698","span":{"begin":385,"end":398},"obj":"Transcription"},{"id":"T15697","span":{"begin":367,"end":374},"obj":"Protein"},{"id":"T15696","span":{"begin":324,"end":332},"obj":"Positive_regulation"},{"id":"T15695","span":{"begin":294,"end":302},"obj":"Protein"},{"id":"T15694","span":{"begin":271,"end":284},"obj":"Transcription"}],"relations":[{"id":"R11585","pred":"themeOf","subj":"T15689","obj":"T15690"},{"id":"R11586","pred":"themeOf","subj":"T15691","obj":"T15690"},{"id":"R11587","pred":"themeOf","subj":"T15692","obj":"T15690"},{"id":"R11588","pred":"themeOf","subj":"T15695","obj":"T15694"},{"id":"R11589","pred":"themeOf","subj":"T15699","obj":"T15698"},{"id":"R18565","pred":"causeOf","subj":"T26053","obj":"T26054"},{"id":"R18566","pred":"themeOf","subj":"T26063","obj":"T26062"},{"id":"R18567","pred":"causeOf","subj":"T26069","obj":"T26071"},{"id":"R18568","pred":"themeOf","subj":"T26070","obj":"T26069"},{"id":"R18569","pred":"themeOf","subj":"T26072","obj":"T26071"},{"id":"R18570","pred":"themeOf","subj":"T26076","obj":"T26075"},{"id":"R18571","pred":"themeOf","subj":"T26085","obj":"T26084"}],"text":"C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity\nSince C/EBP-β binds to the SerpinB2 proximal promoter in an LPS-inducible manner both in vitro and in vivo, we wanted to address the question of whether C/EBP-β was an essential factor for driving transcription from the SerpinB2 promoter in cells in response to LPS. The ability of endogenous C/EBP-β to direct transcription from the SerpinB2 proximal promoter was examined by transfection of the pGLmP-539 murine SerpinB2 luciferase reporter construct into Cebpb+/+ and Cebpb−/− MEFs. We found that LPS-stimulated SerpinB2 promoter activity was significantly increased in Cebpb+/+ MEFs and abrogated in Cebpb−/− MEFs (Fig. 8A), indicating that endogenous C/EBP-β is required for LPS-induced SerpinB2 proximal promoter activity.\n10.1371/journal.pone.0057855.g008 Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity.\n(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA)."}