PMC:3589482 / 13058-13321
Annnotations
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7058 | 262-263 | . | denotes | . |
T7057 | 261-262 | -RRB- | denotes | ) |
T7056 | 251-261 | NNP | denotes | Invitrogen |
T7055 | 250-251 | -LRB- | denotes | ( |
T7054 | 242-249 | NN | denotes | reagent |
T7053 | 237-241 | CD | denotes | 2000 |
T7052 | 223-236 | NNP | denotes | Lipofectamine |
T7051 | 217-222 | VBG | denotes | using |
T7050 | 215-216 | -RRB- | denotes | ) |
T7049 | 213-215 | NN | denotes | µg |
T7048 | 208-212 | CD | denotes | 0.25 |
T7047 | 207-208 | -LRB- | denotes | ( |
T7046 | 199-206 | NN | denotes | plasmid |
T7045 | 190-198 | NN | denotes | envelope |
T7044 | 179-189 | NNP | denotes | pCMV-VSV-G |
T7043 | 175-178 | CC | denotes | and |
T7042 | 173-174 | , | denotes | , |
T7041 | 172-173 | -RRB- | denotes | ) |
T7040 | 170-172 | NN | denotes | µg |
T7039 | 165-169 | CD | denotes | 0.75 |
T7038 | 164-165 | -LRB- | denotes | ( |
T7037 | 156-163 | NN | denotes | plasmid |
T7036 | 146-155 | NN | denotes | packaging |
T7035 | 141-145 | NN | denotes | dvpr |
T7034 | 139-141 | CD | denotes | .2 |
T7033 | 131-139 | JJ | denotes | pCMV-ΔR8 |
T7032 | 129-130 | , | denotes | , |
T7031 | 128-129 | -RRB- | denotes | ) |
T7030 | 126-128 | NN | denotes | µg |
T7029 | 124-125 | CD | denotes | 1 |
T7028 | 123-124 | -LRB- | denotes | ( |
T7027 | 115-122 | NN | denotes | plasmid |
T7026 | 104-114 | NN | denotes | expression |
T7025 | 98-103 | NN | denotes | shRNA |
T7024 | 93-97 | DT | denotes | each |
T7023 | 91-92 | : | denotes | : |
T7022 | 83-91 | NNS | denotes | plasmids |
T7021 | 80-82 | IN | denotes | of |
T7020 | 72-79 | NN | denotes | mixture |
T7019 | 70-71 | DT | denotes | a |
T7018 | 65-69 | IN | denotes | with |
T7017 | 53-64 | VBN | denotes | transfected |
T7016 | 48-52 | VBD | denotes | were |
T7015 | 42-47 | NNS | denotes | cells |
T7014 | 33-41 | JJ | denotes | HEK-293T |
T7013 | 31-32 | , | denotes | , |
T7012 | 22-31 | NNS | denotes | particles |
T7011 | 11-21 | JJ | denotes | lentiviral |
T7010 | 3-10 | VB | denotes | produce |
T7009 | 0-2 | TO | denotes | To |
R5051 | T7009 | T7010 | aux | To,produce |
R5052 | T7010 | T7017 | advcl | produce,transfected |
R5053 | T7011 | T7012 | amod | lentiviral,particles |
R5054 | T7012 | T7010 | dobj | particles,produce |
R5055 | T7013 | T7017 | punct | ",",transfected |
R5056 | T7014 | T7015 | amod | HEK-293T,cells |
R5057 | T7015 | T7017 | nsubjpass | cells,transfected |
R5058 | T7016 | T7017 | auxpass | were,transfected |
R5059 | T7017 | T7017 | ROOT | transfected,transfected |
R5060 | T7018 | T7017 | prep | with,transfected |
R5061 | T7019 | T7020 | det | a,mixture |
R5062 | T7020 | T7018 | pobj | mixture,with |
R5063 | T7021 | T7020 | prep | of,mixture |
R5064 | T7022 | T7021 | pobj | plasmids,of |
R5065 | T7023 | T7017 | punct | :,transfected |
R5066 | T7024 | T7027 | det | each,plasmid |
R5067 | T7025 | T7027 | amod | shRNA,plasmid |
R5068 | T7026 | T7027 | compound | expression,plasmid |
R5069 | T7027 | T7027 | ROOT | plasmid,plasmid |
R5070 | T7028 | T7030 | punct | (,µg |
R5071 | T7029 | T7030 | nummod | 1,µg |
R5072 | T7030 | T7027 | appos | µg,plasmid |
R5073 | T7031 | T7027 | punct | ),plasmid |
R5074 | T7032 | T7027 | punct | ",",plasmid |
R5075 | T7033 | T7037 | amod | pCMV-ΔR8,plasmid |
R5076 | T7034 | T7037 | nummod | .2,plasmid |
R5077 | T7035 | T7037 | compound | dvpr,plasmid |
R5078 | T7036 | T7037 | compound | packaging,plasmid |
R5079 | T7037 | T7027 | conj | plasmid,plasmid |
R5080 | T7038 | T7037 | punct | (,plasmid |
R5081 | T7039 | T7040 | nummod | 0.75,µg |
R5082 | T7040 | T7037 | appos | µg,plasmid |
R5083 | T7041 | T7037 | punct | ),plasmid |
R5084 | T7042 | T7037 | punct | ",",plasmid |
R5085 | T7043 | T7017 | cc | and,transfected |
R5086 | T7044 | T7046 | compound | pCMV-VSV-G,plasmid |
R5087 | T7045 | T7046 | compound | envelope,plasmid |
R5088 | T7046 | T7046 | ROOT | plasmid,plasmid |
R5089 | T7047 | T7046 | punct | (,plasmid |
R5090 | T7048 | T7049 | nummod | 0.25,µg |
R5091 | T7049 | T7046 | appos | µg,plasmid |
R5092 | T7050 | T7046 | punct | ),plasmid |
R5093 | T7051 | T7017 | advcl | using,transfected |
R5094 | T7052 | T7054 | nmod | Lipofectamine,reagent |
R5095 | T7053 | T7054 | nummod | 2000,reagent |
R5096 | T7054 | T7051 | dobj | reagent,using |
R5097 | T7055 | T7054 | punct | (,reagent |
R5098 | T7056 | T7054 | appos | Invitrogen,reagent |
R5099 | T7057 | T7054 | punct | ),reagent |
R5100 | T7058 | T7017 | punct | .,transfected |
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6788 | 261-262 | -RRB- | denotes | ) |
T6787 | 251-261 | NN | denotes | Invitrogen |
T6786 | 250-251 | -LRB- | denotes | ( |
T6785 | 242-249 | NN | denotes | reagent |
T6784 | 237-241 | CD | denotes | 2000 |
T6783 | 223-236 | NN | denotes | Lipofectamine |
T6782 | 217-222 | VB | denotes | using |
T6781 | 215-216 | -RRB- | denotes | ) |
T6780 | 213-215 | NN | denotes | µg |
T6779 | 208-212 | CD | denotes | 0.25 |
T6778 | 207-208 | -LRB- | denotes | ( |
T6777 | 199-206 | NN | denotes | plasmid |
T6776 | 190-198 | NN | denotes | envelope |
T6775 | 179-189 | NN | denotes | pCMV-VSV-G |
T6774 | 175-178 | CC | denotes | and |
T6773 | 173-174 | -COMMA- | denotes | , |
T6772 | 172-173 | -RRB- | denotes | ) |
T6771 | 170-172 | NN | denotes | µg |
T6770 | 165-169 | CD | denotes | 0.75 |
T6769 | 164-165 | -LRB- | denotes | ( |
T6768 | 156-163 | NN | denotes | plasmid |
T6767 | 146-155 | NN | denotes | packaging |
T6766 | 131-145 | NN | denotes | pCMV-ΔR8.2dvpr |
T6765 | 129-130 | -COMMA- | denotes | , |
T6764 | 128-129 | -RRB- | denotes | ) |
T6763 | 126-128 | NN | denotes | µg |
T6762 | 124-125 | CD | denotes | 1 |
T6761 | 123-124 | -LRB- | denotes | ( |
T6760 | 115-122 | NN | denotes | plasmid |
T6759 | 104-114 | NN | denotes | expression |
T6758 | 98-103 | NN | denotes | shRNA |
T6757 | 93-97 | DT | denotes | each |
T6756 | 91-92 | -COLON- | denotes | : |
T6755 | 83-91 | NN | denotes | plasmids |
T6754 | 80-82 | IN | denotes | of |
T6753 | 72-79 | NN | denotes | mixture |
T6752 | 70-71 | DT | denotes | a |
T6751 | 65-69 | IN | denotes | with |
T6750 | 53-64 | VB | denotes | transfected |
T6749 | 48-52 | VB | denotes | were |
T6748 | 42-47 | NN | denotes | cells |
T6747 | 33-41 | NN | denotes | HEK-293T |
T6746 | 31-32 | -COMMA- | denotes | , |
T6745 | 22-31 | NN | denotes | particles |
T6744 | 11-21 | JJ | denotes | lentiviral |
T6743 | 3-10 | VB | denotes | produce |
T6742 | 0-2 | TO | denotes | To |
R4823 | T6743 | T6742 | arg1Of | produce,To |
R4824 | T6745 | T6743 | arg2Of | particles,produce |
R4825 | T6745 | T6744 | arg1Of | particles,lentiviral |
R4826 | T6748 | T6743 | arg1Of | cells,produce |
R4827 | T6748 | T6747 | arg1Of | cells,HEK-293T |
R4828 | T6748 | T6749 | arg1Of | cells,were |
R4829 | T6748 | T6750 | arg2Of | cells,transfected |
R4830 | T6748 | T6782 | arg1Of | cells,using |
R4831 | T6750 | T6742 | modOf | transfected,To |
R4832 | T6750 | T6746 | arg1Of | transfected,"," |
R4833 | T6750 | T6749 | arg2Of | transfected,were |
R4834 | T6750 | T6751 | arg1Of | transfected,with |
R4835 | T6750 | T6782 | modOf | transfected,using |
R4836 | T6753 | T6751 | arg2Of | mixture,with |
R4837 | T6753 | T6752 | arg1Of | mixture,a |
R4838 | T6753 | T6754 | arg1Of | mixture,of |
R4839 | T6753 | T6756 | arg1Of | mixture,: |
R4840 | T6755 | T6754 | arg2Of | plasmids,of |
R4841 | T6760 | T6758 | arg1Of | plasmid,shRNA |
R4842 | T6760 | T6759 | arg1Of | plasmid,expression |
R4843 | T6760 | T6761 | arg1Of | plasmid,( |
R4844 | T6760 | T6765 | arg1Of | plasmid,"," |
R4845 | T6763 | T6761 | arg2Of | µg,( |
R4846 | T6763 | T6762 | arg1Of | µg,1 |
R4847 | T6764 | T6761 | arg3Of | ),( |
R4848 | T6765 | T6774 | arg1Of | ",",and |
R4849 | T6768 | T6765 | arg2Of | plasmid,"," |
R4850 | T6768 | T6766 | arg1Of | plasmid,pCMV-ΔR8.2dvpr |
R4851 | T6768 | T6767 | arg1Of | plasmid,packaging |
R4852 | T6768 | T6769 | arg1Of | plasmid,( |
R4853 | T6771 | T6769 | arg2Of | µg,( |
R4854 | T6771 | T6770 | arg1Of | µg,0.75 |
R4855 | T6772 | T6769 | arg3Of | ),( |
R4856 | T6774 | T6756 | arg2Of | and,: |
R4857 | T6774 | T6757 | arg1Of | and,each |
R4858 | T6774 | T6773 | arg1Of | and,"," |
R4859 | T6777 | T6774 | arg2Of | plasmid,and |
R4860 | T6777 | T6775 | arg1Of | plasmid,pCMV-VSV-G |
R4861 | T6777 | T6776 | arg1Of | plasmid,envelope |
R4862 | T6777 | T6778 | arg1Of | plasmid,( |
R4863 | T6780 | T6778 | arg2Of | µg,( |
R4864 | T6780 | T6779 | arg1Of | µg,0.25 |
R4865 | T6781 | T6778 | arg3Of | ),( |
R4866 | T6785 | T6782 | arg2Of | reagent,using |
R4867 | T6785 | T6783 | arg1Of | reagent,Lipofectamine |
R4868 | T6785 | T6784 | arg1Of | reagent,2000 |
R4869 | T6785 | T6786 | arg1Of | reagent,( |
R4870 | T6787 | T6786 | arg2Of | Invitrogen,( |
R4871 | T6788 | T6786 | arg3Of | ),( |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6656 | 190-198 | http://purl.obolibrary.org/obo/GO_0009274 | denotes | envelope |
T6654 | 42-47 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6635 | 0-263 | Sentence | denotes | To produce lentiviral particles, HEK-293T cells were transfected with a mixture of plasmids: each shRNA expression plasmid (1 µg), pCMV-ΔR8.2dvpr packaging plasmid (0.75 µg), and pCMV-VSV-G envelope plasmid (0.25 µg) using Lipofectamine 2000 reagent (Invitrogen). |
T79 | 0-263 | Sentence | denotes | To produce lentiviral particles, HEK-293T cells were transfected with a mixture of plasmids: each shRNA expression plasmid (1 µg), pCMV-ΔR8.2dvpr packaging plasmid (0.75 µg), and pCMV-VSV-G envelope plasmid (0.25 µg) using Lipofectamine 2000 reagent (Invitrogen). |
bionlp-st-ge-2016-test-ihmc
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6923 | 223-262 | Entity | denotes | Lipofectamine 2000 reagent (Invitrogen) |
T6914 | 11-32 | Entity | denotes | lentiviral particles, |
T6909 | 65-91 | Entity | denotes | with a mixture of plasmids |
testone
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6619 | 3-10 | Gene_expression | denotes | produce |