PMC:3585731 / 61919-62480 JSONTXT

{"project":"sentences","denotations":[{"id":"T416","span":{"begin":205,"end":255},"obj":"Sentence"},{"id":"T417","span":{"begin":256,"end":362},"obj":"Sentence"},{"id":"T418","span":{"begin":363,"end":420},"obj":"Sentence"},{"id":"T419","span":{"begin":421,"end":469},"obj":"Sentence"},{"id":"T420","span":{"begin":470,"end":521},"obj":"Sentence"},{"id":"T421","span":{"begin":522,"end":560},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"PI3-Kinase and PDK1 mediate the increase in Akt Thr308 phosphorylation under necroptotic conditions. (A-D) L929 cells were stimulated by zVAD.fmk and TNFα in the presence of LY249002 (A,B) or BX912 (C,D). Viability assays were performed after 24 hr (A,C). Western blot samples were collected after 9 hr. (B,D). (E,F) L929 cells were transfected with PDK1 siRNAs. After 72 hr, necroptosis was induced by TNFα or zVAD.fmk. Viability assays were performed after 24 hr (E). Western blot samples were collected after 9 hr (F). In all graphs, average±SD was plotted.\n"}