PMC:3585731 / 60162-60796 JSONTXT

{"project":"sentences","denotations":[{"id":"T401","span":{"begin":322,"end":363},"obj":"Sentence"},{"id":"T402","span":{"begin":364,"end":463},"obj":"Sentence"},{"id":"T403","span":{"begin":464,"end":532},"obj":"Sentence"},{"id":"T404","span":{"begin":533,"end":594},"obj":"Sentence"},{"id":"T405","span":{"begin":595,"end":633},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Downstream Akt signaling contributes to the control of necroptosis. (A,B) L929 were stimulated with zVAD.fmk (A) TNFα (B) for the indicated periods of time, followed by western blot using indicated antibodies. (C,D) L929 were stimulated with TNFα or zVAD.fmk in the presence of the indicated concentrations of PF-4706871. Viability was determined after 24 hr (C). Western blot samples were collected after 9 hr (D). (E) L929 cells were transfected with S6 siRNAs. After 48 hr, necroptosis was induced by TNFα and zVAD.fmk for 24 hr. Inset, levels of S6 were determined 48 hr after transfection. In all graphs, average±SD was plotted.\n"}