PMC:3585731 / 58999-59693 JSONTXT

{"project":"sentences","denotations":[{"id":"T391","span":{"begin":477,"end":564},"obj":"Sentence"},{"id":"T392","span":{"begin":565,"end":608},"obj":"Sentence"},{"id":"T393","span":{"begin":609,"end":654},"obj":"Sentence"},{"id":"T394","span":{"begin":655,"end":693},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"RIP1 kinase-dependent increase in Akt Thr308 phosphorylation during necroptosis. (A, B) L929 cells treated with zVAD.fmk (A) or TNFα (B) for the indicated period of time followed by assessment of cell viability. (C) Cells were serum starved followed by treatment with IGF alone or IGF/zVAD.fmk and samples were collected at the indicated time points for western blot. (D,E) L929 cells were stimulated with bFGF and/or zVAD.fmk and Nec-1 (N1) for the indicated periods of time. Samples were analyzed using phospho-Thr308, phospho-Ser473 and pan-Akt ELISAOne assays. Phospho-signals were normalized to pan-Akt. Fold induction over control cells is plotted. In all graphs, average±SD was plotted.\n"}