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PMC:3585731 / 56512-57077 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T28058 558-564 NN denotes values
T28057 550-557 NN denotes pan-Akt
T28056 547-549 IN denotes on
T28055 541-546 VB denotes based
T28054 530-540 VB denotes normalized
T28053 525-529 VB denotes were
T28052 506-524 NN denotes phospho-antibodies
T28051 502-505 IN denotes for
T28050 494-501 NNP denotes Signals
T28049 489-493 NNP denotes 4°C.
T28048 486-488 IN denotes at
T28047 476-485 RB denotes overnight
T28046 466-475 VB denotes incubated
T28045 462-465 VB denotes was
T28044 454-461 NN denotes pan-Akt
T28043 451-453 TO denotes to
T28042 442-450 NN denotes antibody
T28041 434-441 JJ denotes Primary
T28040 421-432 NN denotes temperature
T28039 416-420 NN denotes room
T28038 413-415 IN denotes at
T28037 410-412 NN denotes hr
T28036 408-409 CD denotes 2
T28035 404-407 IN denotes for
T28034 396-403 NN denotes samples
T28033 392-395 DT denotes the
T28032 387-391 IN denotes with
T28031 377-386 VB denotes incubated
T28030 372-376 VB denotes were
T28029 357-371 NN denotes phopsho-Ser473
T28010 239-241 IN denotes of
T28009 227-238 NN denotes microliters
T28008 222-226 CD denotes Five
T28007 215-220 NN denotes blots
T28006 207-214 JJ denotes Western
T28005 203-206 IN denotes for
T28004 193-202 VB denotes described
T28003 190-192 IN denotes as
T28002 183-189 NN denotes buffer
T28001 178-182 NN denotes RIPA
T28000 175-177 IN denotes in
T27999 166-174 VB denotes prepared
T27998 161-165 VB denotes were
T27997 153-160 NN denotes lysates
T27996 148-152 NN denotes Cell
T27995 133-146 NN denotes modifications
T27994 123-132 VB denotes following
T27993 119-122 DT denotes the
T27992 114-118 IN denotes with
T27991 105-113 NN denotes protocol
T27990 90-104 NN denotes manufacturer’s
T27989 87-89 TO denotes to
T27988 77-86 VB denotes according
T27987 67-76 VB denotes performed
T27986 62-66 VB denotes were
T27985 60-61 -RRB- denotes )
T27984 51-60 NNP denotes Australia
T27983 49-50 -COMMA- denotes ,
T27982 40-49 NNP denotes Hindmarsh
T27981 38-39 -COMMA- denotes ,
T27980 32-38 NNP denotes TGRBio
T27979 31-32 -LRB- denotes (
T27978 24-30 NN denotes assays
T27977 15-23 NN denotes ELISAOne
T27976 9-14 NN denotes Assay
T28028 353-356 CC denotes and
T28027 338-352 NN denotes phopsho-Thr308
T28026 335-337 TO denotes to
T28025 324-334 NN denotes antibodies
T28024 316-323 JJ denotes Primary
T28023 306-314 NN denotes analysis
T28022 303-305 TO denotes to
T28021 297-302 JJ denotes prior
T28020 290-296 NN denotes buffer
T28019 284-289 NN denotes lysis
T28018 275-283 NN denotes ELISAOne
T28017 272-274 IN denotes of
T28016 269-271 NN denotes µL
T28015 266-268 CD denotes 45
T28014 263-265 IN denotes in
T28013 255-262 VB denotes diluted
T28012 250-254 VB denotes were
T28011 242-249 NN denotes samples
T27975 0-8 NN denotes ELISAOne
R19367 T27976 T27975 arg1Of Assay,ELISAOne
R19368 T27978 T27977 arg1Of assays,ELISAOne
R19369 T27978 T27979 arg1Of assays,(
R19370 T27978 T27986 arg1Of assays,were
R19371 T27978 T27987 arg2Of assays,performed
R19372 T27980 T27981 arg1Of TGRBio,","
R19373 T27981 T27979 arg2Of ",",(
R19374 T27981 T27983 arg1Of ",",","
R19375 T27982 T27981 arg2Of Hindmarsh,","
R19376 T27984 T27983 arg2Of Australia,","
R19377 T27985 T27979 arg3Of ),(
R19378 T27987 T27986 arg2Of performed,were
R19379 T27987 T27988 arg1Of performed,according
R19380 T27989 T27988 arg2Of to,according
R19381 T27991 T27989 arg2Of protocol,to
R19382 T27991 T27990 arg1Of protocol,manufacturer’s
R19383 T27991 T27992 arg1Of protocol,with
R19384 T27995 T27992 arg2Of modifications,with
R19385 T27995 T27993 arg1Of modifications,the
R19386 T27995 T27994 arg1Of modifications,following
R19387 T27997 T27996 arg1Of lysates,Cell
R19388 T27997 T27998 arg1Of lysates,were
R19389 T27997 T27999 arg2Of lysates,prepared
R19390 T27999 T27998 arg2Of prepared,were
R19391 T27999 T28000 arg1Of prepared,in
R19392 T27999 T28003 arg1Of prepared,as
R19393 T28002 T28000 arg2Of buffer,in
R19394 T28002 T28001 arg1Of buffer,RIPA
R19395 T28004 T28003 arg2Of described,as
R19396 T28004 T28005 arg1Of described,for
R19397 T28007 T28005 arg2Of blots,for
R19398 T28007 T28006 arg1Of blots,Western
R19399 T28009 T28008 arg1Of microliters,Five
R19400 T28009 T28010 arg1Of microliters,of
R19401 T28009 T28012 arg1Of microliters,were
R19402 T28009 T28013 arg2Of microliters,diluted
R19403 T28011 T28010 arg2Of samples,of
R19404 T28013 T28012 arg2Of diluted,were
R19405 T28013 T28014 arg1Of diluted,in
R19406 T28016 T28014 arg2Of µL,in
R19407 T28016 T28015 arg1Of µL,45
R19408 T28016 T28017 arg1Of µL,of
R19409 T28020 T28017 arg2Of buffer,of
R19410 T28020 T28018 arg1Of buffer,ELISAOne
R19411 T28020 T28019 arg1Of buffer,lysis
R19412 T28020 T28021 arg1Of buffer,prior
R19413 T28021 T28022 arg1Of prior,to
R19414 T28023 T28022 arg2Of analysis,to
R19415 T28025 T28024 arg1Of antibodies,Primary
R19416 T28025 T28026 arg1Of antibodies,to
R19417 T28025 T28030 arg1Of antibodies,were
R19418 T28025 T28031 arg2Of antibodies,incubated
R19419 T28027 T28028 arg1Of phopsho-Thr308,and
R19420 T28028 T28026 arg2Of and,to
R19421 T28029 T28028 arg2Of phopsho-Ser473,and
R19422 T28031 T28030 arg2Of incubated,were
R19423 T28031 T28032 arg1Of incubated,with
R19424 T28034 T28032 arg2Of samples,with
R19425 T28034 T28033 arg1Of samples,the
R19426 T28034 T28035 arg1Of samples,for
R19427 T28037 T28035 arg2Of hr,for
R19428 T28037 T28036 arg1Of hr,2
R19429 T28037 T28038 arg1Of hr,at
R19430 T28040 T28038 arg2Of temperature,at
R19431 T28040 T28039 arg1Of temperature,room
R19432 T28042 T28041 arg1Of antibody,Primary
R19433 T28042 T28043 arg1Of antibody,to
R19434 T28042 T28045 arg1Of antibody,was
R19435 T28042 T28046 arg2Of antibody,incubated
R19436 T28044 T28043 arg2Of pan-Akt,to
R19437 T28046 T28045 arg2Of incubated,was
R19438 T28046 T28047 arg1Of incubated,overnight
R19439 T28046 T28048 arg1Of incubated,at
R19440 T28046 T28051 arg1Of incubated,for
R19441 T28050 T28048 arg2Of Signals,at
R19442 T28050 T28049 arg1Of Signals,4°C.
R19443 T28052 T28053 arg1Of phospho-antibodies,were
R19444 T28052 T28054 arg2Of phospho-antibodies,normalized
R19445 T28054 T28051 arg2Of normalized,for
R19446 T28054 T28053 arg2Of normalized,were
R19447 T28054 T28055 arg1Of normalized,based
R19448 T28056 T28055 arg2Of on,based
R19449 T28058 T28056 arg2Of values,on
R19450 T28058 T28057 arg1Of values,pan-Akt

GO-BP

Id Subject Object Predicate Lexical cue
T27967 494-501 http://purl.obolibrary.org/obo/GO_0023052 denotes Signals
T27966 284-289 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

GO-MF

Id Subject Object Predicate Lexical cue
T27969 514-524 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27968 324-334 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T27974 514-524 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27973 324-334 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27972 514-524 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27971 324-334 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27970 148-152 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell

sentences

Id Subject Object Predicate Lexical cue
T27965 434-565 Sentence denotes Primary antibody to pan-Akt was incubated overnight at 4°C. Signals for phospho-antibodies were normalized based on pan-Akt values.
T27964 316-433 Sentence denotes Primary antibodies to phopsho-Thr308 and phopsho-Ser473 were incubated with the samples for 2 hr at room temperature.
T27963 222-315 Sentence denotes Five microliters of samples were diluted in 45 µL of ELISAOne lysis buffer prior to analysis.
T27962 148-221 Sentence denotes Cell lysates were prepared in RIPA buffer as described for Western blots.
T27961 15-147 Sentence denotes ELISAOne assays (TGRBio, Hindmarsh, Australia) were performed according to manufacturer’s protocol with the following modifications.
T27960 0-14 Sentence denotes ELISAOne Assay
T365 0-14 Sentence denotes ELISAOne Assay
T366 15-147 Sentence denotes ELISAOne assays (TGRBio, Hindmarsh, Australia) were performed according to manufacturer’s protocol with the following modifications.
T367 148-221 Sentence denotes Cell lysates were prepared in RIPA buffer as described for Western blots.
T368 222-315 Sentence denotes Five microliters of samples were diluted in 45 µL of ELISAOne lysis buffer prior to analysis.
T369 316-433 Sentence denotes Primary antibodies to phopsho-Thr308 and phopsho-Ser473 were incubated with the samples for 2 hr at room temperature.
T370 434-493 Sentence denotes Primary antibody to pan-Akt was incubated overnight at 4°C.
T371 494-565 Sentence denotes Signals for phospho-antibodies were normalized based on pan-Akt values.

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T28069 148-220 Entity denotes Cell lysates were prepared in RIPA buffer as described for Western blots
T28068 494-501 Entity denotes Signals
T28067 357-371 Entity denotes phopsho-Ser473
T28066 550-564 Protein denotes pan-Akt values
T28065 454-461 Protein denotes pan-Akt
T28064 434-450 Entity denotes Primary antibody
T28063 506-524 Entity denotes phospho-antibodies
T28062 32-35 Protein denotes TGR
T28061 15-61 Entity denotes ELISAOne assays (TGRBio, Hindmarsh, Australia)
T28060 338-352 Entity denotes phopsho-Thr308
T28059 316-371 Entity denotes Primary antibodies to phopsho-Thr308 and phopsho-Ser473

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T28160 564-565 . denotes .
T28159 558-564 NNS denotes values
T28158 550-557 JJ denotes pan-Akt
T28157 547-549 IN denotes on
T28156 541-546 VBN denotes based
T28155 530-540 VBN denotes normalized
T28154 525-529 VBD denotes were
T28153 506-524 NNS denotes phospho-antibodies
T28152 502-505 IN denotes for
T28151 494-501 NNP denotes Signals
T28150 491-493 NNP denotes C.
T28149 490-491 CD denotes °
T28148 489-490 CD denotes 4
T28147 486-488 IN denotes at
T28146 476-485 RB denotes overnight
T28145 466-475 VBN denotes incubated
T28144 462-465 VBD denotes was
T28143 454-461 NN denotes pan-Akt
T28142 451-453 TO denotes to
T28141 442-450 NN denotes antibody
T28140 434-441 JJ denotes Primary
T28139 432-433 . denotes .
T28138 421-432 NN denotes temperature
T28137 416-420 NN denotes room
T28136 413-415 IN denotes at
T28135 410-412 NN denotes hr
T28134 408-409 CD denotes 2
T28133 404-407 IN denotes for
T28132 396-403 NNS denotes samples
T28131 392-395 DT denotes the
T28130 387-391 IN denotes with
T28129 377-386 VBN denotes incubated
T28128 372-376 VBD denotes were
T28127 357-371 JJ denotes phopsho-Ser473
T28126 353-356 CC denotes and
T28125 338-352 JJ denotes phopsho-Thr308
T28124 335-337 TO denotes to
T28123 324-334 NNS denotes antibodies
T28122 316-323 JJ denotes Primary
T28121 314-315 . denotes .
T28120 306-314 NN denotes analysis
T28119 303-305 TO denotes to
T28118 297-302 RB denotes prior
T28117 290-296 NN denotes buffer
T28116 284-289 VBZ denotes lysis
T28107 239-241 IN denotes of
T28106 227-238 NNS denotes microliters
T28105 222-226 CD denotes Five
T28104 220-221 . denotes .
T28103 215-220 NNS denotes blots
T28102 207-214 JJ denotes Western
T28101 203-206 IN denotes for
T28100 193-202 VBN denotes described
T28099 190-192 IN denotes as
T28098 183-189 NN denotes buffer
T28097 178-182 NNP denotes RIPA
T28096 175-177 IN denotes in
T28095 166-174 VBN denotes prepared
T28094 161-165 VBD denotes were
T28093 153-160 NNS denotes lysates
T28092 148-152 NN denotes Cell
T28091 146-147 . denotes .
T28090 133-146 NNS denotes modifications
T28089 123-132 VBG denotes following
T28088 119-122 DT denotes the
T28087 114-118 IN denotes with
T28086 105-113 NN denotes protocol
T28085 90-102 NN denotes manufacturer
T28084 87-89 TO denotes to
T28083 77-86 VBG denotes according
T28082 67-76 VBN denotes performed
T28081 62-66 VBD denotes were
T28080 60-61 -RRB- denotes )
T28079 51-60 NNP denotes Australia
T28078 49-50 , denotes ,
T28077 40-49 NNP denotes Hindmarsh
T28076 38-39 , denotes ,
T28075 32-38 NNP denotes TGRBio
T28074 31-32 -LRB- denotes (
T28073 24-30 NNS denotes assays
T28072 15-23 NNP denotes ELISAOne
T28071 9-14 NNP denotes Assay
T28070 0-8 NNP denotes ELISAOne
T28115 275-283 NNP denotes ELISAOne
T28114 272-274 IN denotes of
T28113 269-271 NN denotes µL
T28112 266-268 CD denotes 45
T28111 263-265 IN denotes in
T28110 255-262 VBN denotes diluted
T28109 250-254 VBD denotes were
T28108 242-249 NNS denotes samples
R19451 T28070 T28072 compound ELISAOne,ELISAOne
R19452 T28071 T28072 compound Assay,ELISAOne
R19453 T28072 T28073 compound ELISAOne,assays
R19454 T28073 T28073 ROOT assays,assays
R19455 T28074 T28082 punct (,performed
R19456 T28075 T28082 nsubjpass TGRBio,performed
R19457 T28076 T28075 punct ",",TGRBio
R19458 T28077 T28075 conj Hindmarsh,TGRBio
R19459 T28078 T28077 punct ",",Hindmarsh
R19460 T28079 T28077 appos Australia,Hindmarsh
R19461 T28080 T28077 punct ),Hindmarsh
R19462 T28081 T28082 auxpass were,performed
R19463 T28082 T28073 relcl performed,assays
R19464 T28083 T28082 prep according,performed
R19465 T28084 T28083 prep to,according
R19466 T28085 T28086 poss manufacturer,protocol
R19467 T28086 T28084 pobj protocol,to
R19468 T28087 T28082 prep with,performed
R19469 T28088 T28090 det the,modifications
R19470 T28089 T28090 amod following,modifications
R19471 T28090 T28087 pobj modifications,with
R19472 T28091 T28082 punct .,performed
R19473 T28092 T28093 compound Cell,lysates
R19474 T28093 T28095 nsubjpass lysates,prepared
R19475 T28094 T28095 auxpass were,prepared
R19476 T28095 T28095 ROOT prepared,prepared
R19477 T28096 T28095 prep in,prepared
R19478 T28097 T28098 compound RIPA,buffer
R19479 T28098 T28096 pobj buffer,in
R19480 T28099 T28100 mark as,described
R19481 T28100 T28095 advcl described,prepared
R19482 T28101 T28100 prep for,described
R19483 T28102 T28103 amod Western,blots
R19484 T28103 T28101 pobj blots,for
R19485 T28104 T28095 punct .,prepared
R19486 T28105 T28106 nummod Five,microliters
R19487 T28106 T28110 nsubjpass microliters,diluted
R19488 T28107 T28106 prep of,microliters
R19489 T28108 T28107 pobj samples,of
R19490 T28109 T28110 auxpass were,diluted
R19491 T28110 T28110 ROOT diluted,diluted
R19492 T28111 T28110 prep in,diluted
R19493 T28112 T28113 nummod 45,µL
R19494 T28113 T28111 pobj µL,in
R19495 T28114 T28113 prep of,µL
R19496 T28115 T28116 compound ELISAOne,lysis
R19497 T28116 T28117 compound lysis,buffer
R19498 T28117 T28114 pobj buffer,of
R19499 T28118 T28110 advmod prior,diluted
R19500 T28119 T28118 prep to,prior
R19501 T28120 T28119 pobj analysis,to
R19502 T28121 T28110 punct .,diluted
R19503 T28122 T28123 amod Primary,antibodies
R19504 T28123 T28129 nsubjpass antibodies,incubated
R19505 T28124 T28123 prep to,antibodies
R19506 T28125 T28124 pobj phopsho-Thr308,to
R19507 T28126 T28125 cc and,phopsho-Thr308
R19508 T28127 T28125 conj phopsho-Ser473,phopsho-Thr308
R19509 T28128 T28129 auxpass were,incubated
R19510 T28129 T28129 ROOT incubated,incubated
R19511 T28130 T28129 prep with,incubated
R19512 T28131 T28132 det the,samples
R19513 T28132 T28130 pobj samples,with
R19514 T28133 T28132 prep for,samples
R19515 T28134 T28135 nummod 2,hr
R19516 T28135 T28133 pobj hr,for
R19517 T28136 T28129 prep at,incubated
R19518 T28137 T28138 compound room,temperature
R19519 T28138 T28136 pobj temperature,at
R19520 T28139 T28129 punct .,incubated
R19521 T28140 T28141 amod Primary,antibody
R19522 T28141 T28145 nsubjpass antibody,incubated
R19523 T28142 T28145 prep to,incubated
R19524 T28143 T28142 pobj pan-Akt,to
R19525 T28144 T28145 auxpass was,incubated
R19526 T28145 T28145 ROOT incubated,incubated
R19527 T28146 T28145 advmod overnight,incubated
R19528 T28147 T28145 prep at,incubated
R19529 T28148 T28149 nummod 4,°
R19530 T28149 T28151 nummod °,Signals
R19531 T28150 T28151 compound C.,Signals
R19532 T28151 T28147 pobj Signals,at
R19533 T28152 T28145 prep for,incubated
R19534 T28153 T28152 pobj phospho-antibodies,for
R19535 T28154 T28145 auxpass were,incubated
R19536 T28155 T28154 acomp normalized,were
R19537 T28156 T28155 prep based,normalized
R19538 T28157 T28156 prep on,based
R19539 T28158 T28159 amod pan-Akt,values
R19540 T28159 T28157 pobj values,on
R19541 T28160 T28145 punct .,incubated