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PMC:3585731 / 56105-56510 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T27857 395-404 NN denotes puromycin
T27856 389-394 NN denotes µg/ml
T27855 386-388 CD denotes 10
T27854 383-385 IN denotes in
T27853 372-382 VB denotes maintained
T27852 368-371 CC denotes and
T27851 359-367 VB denotes selected
T27850 354-358 VB denotes were
T27849 348-353 NN denotes Cells
T27848 337-346 NN denotes polybrene
T27847 331-336 NN denotes µg/ml
T27846 329-330 CD denotes 8
T27845 324-328 IN denotes with
T27844 318-323 NN denotes cells
T27843 313-317 NN denotes L929
T27842 310-312 TO denotes to
T27841 302-309 VB denotes applied
T27840 298-301 CC denotes and
T27839 291-297 NN denotes filter
T27838 288-290 NN denotes µm
T27837 283-287 CD denotes 0.45
T27836 275-282 IN denotes through
T27835 266-274 VB denotes filtered
T27834 264-265 -COMMA- denotes ,
T27833 259-264 RB denotes later
T27832 256-258 NN denotes hr
T27831 253-255 CD denotes 72
T27830 243-252 VB denotes collected
T27829 239-242 VB denotes was
T27828 233-238 NN denotes media
T27827 216-232 JJ denotes Virus-containing
T27826 213-214 -RRB- denotes )
T27825 209-213 NN denotes Labs
T27824 200-208 NN denotes Signagen
T27823 199-200 -LRB- denotes (
T27822 191-198 NN denotes reagent
T27821 178-190 NN denotes transfection
T27820 171-177 NN denotes GenJet
T27819 165-170 VB denotes using
T27818 158-164 VB denotes plates
T27817 153-157 RB denotes well
T27816 151-152 CD denotes 6
T27815 148-150 IN denotes in
T27814 139-147 NN denotes plasmids
T27813 129-138 JJ denotes accessory
T27812 123-128 JJ denotes VSV-G
T27811 119-122 CC denotes and
T27810 111-118 NN denotes gal/pol
T27809 108-110 IN denotes of
T27808 105-107 NN denotes µg
T27807 103-104 CD denotes 1
T27806 99-102 CC denotes and
T27805 95-98 NN denotes DNA
T27804 89-94 JJ denotes viral
T27803 86-88 IN denotes of
T27802 83-85 NN denotes µg
T27801 81-82 CD denotes 2
T27800 76-80 IN denotes with
T27799 64-75 VB denotes transfected
T27798 59-63 VB denotes were
T27797 57-58 -RRB- denotes )
T27796 47-57 NN denotes Invitrogen
T27795 46-47 -LRB- denotes (
T27794 40-45 NN denotes cells
T27793 31-39 NN denotes HEK293FT
T27792 29-30 -COMMA- denotes ,
T27791 17-29 NN denotes retroviruses
T27790 12-16 NN denotes MSCV
T27789 3-11 VB denotes generate
T27788 0-2 TO denotes To
R19209 T27789 T27788 arg1Of generate,To
R19210 T27791 T27789 arg2Of retroviruses,generate
R19211 T27791 T27790 arg1Of retroviruses,MSCV
R19212 T27794 T27789 arg1Of cells,generate
R19213 T27794 T27793 arg1Of cells,HEK293FT
R19214 T27794 T27795 arg1Of cells,(
R19215 T27794 T27798 arg1Of cells,were
R19216 T27794 T27799 arg2Of cells,transfected
R19217 T27796 T27795 arg2Of Invitrogen,(
R19218 T27797 T27795 arg3Of ),(
R19219 T27799 T27798 arg2Of transfected,were
R19220 T27799 T27800 arg1Of transfected,with
R19221 T27799 T27806 arg1Of transfected,and
R19222 T27802 T27800 arg2Of µg,with
R19223 T27802 T27801 arg1Of µg,2
R19224 T27802 T27803 arg1Of µg,of
R19225 T27805 T27803 arg2Of DNA,of
R19226 T27805 T27804 arg1Of DNA,viral
R19227 T27806 T27788 modOf and,To
R19228 T27806 T27792 arg1Of and,","
R19229 T27808 T27807 arg1Of µg,1
R19230 T27808 T27809 arg1Of µg,of
R19231 T27808 T27815 arg1Of µg,in
R19232 T27808 T27818 arg1Of µg,plates
R19233 T27808 T27819 arg1Of µg,using
R19234 T27810 T27811 arg1Of gal/pol,and
R19235 T27813 T27811 arg2Of accessory,and
R19236 T27813 T27812 arg1Of accessory,VSV-G
R19237 T27814 T27809 arg2Of plasmids,of
R19238 T27814 T27810 arg1Of plasmids,gal/pol
R19239 T27814 T27813 arg1Of plasmids,accessory
R19240 T27816 T27815 arg2Of 6,in
R19241 T27818 T27806 arg2Of plates,and
R19242 T27818 T27817 arg1Of plates,well
R19243 T27819 T27818 arg2Of using,plates
R19244 T27822 T27819 arg2Of reagent,using
R19245 T27822 T27820 arg1Of reagent,GenJet
R19246 T27822 T27821 arg1Of reagent,transfection
R19247 T27822 T27823 arg1Of reagent,(
R19248 T27825 T27823 arg2Of Labs,(
R19249 T27825 T27824 arg1Of Labs,Signagen
R19250 T27826 T27823 arg3Of ),(
R19251 T27828 T27827 arg1Of media,Virus-containing
R19252 T27828 T27829 arg1Of media,was
R19253 T27828 T27830 arg2Of media,collected
R19254 T27828 T27835 arg2Of media,filtered
R19255 T27828 T27841 arg2Of media,applied
R19256 T27830 T27832 arg1Of collected,hr
R19257 T27830 T27833 arg1Of collected,later
R19258 T27830 T27834 arg1Of collected,","
R19259 T27832 T27831 arg1Of hr,72
R19260 T27834 T27840 arg1Of ",",and
R19261 T27835 T27834 arg2Of filtered,","
R19262 T27835 T27836 arg1Of filtered,through
R19263 T27837 T27838 arg1Of 0.45,µm
R19264 T27839 T27836 arg2Of filter,through
R19265 T27839 T27837 arg1Of filter,0.45
R19266 T27840 T27829 arg2Of and,was
R19267 T27841 T27840 arg2Of applied,and
R19268 T27841 T27842 arg1Of applied,to
R19269 T27844 T27842 arg2Of cells,to
R19270 T27844 T27843 arg1Of cells,L929
R19271 T27844 T27845 arg1Of cells,with
R19272 T27848 T27845 arg2Of polybrene,with
R19273 T27848 T27846 arg1Of polybrene,8
R19274 T27848 T27847 arg1Of polybrene,µg/ml
R19275 T27849 T27850 arg1Of Cells,were
R19276 T27849 T27851 arg2Of Cells,selected
R19277 T27849 T27853 arg2Of Cells,maintained
R19278 T27851 T27852 arg1Of selected,and
R19279 T27852 T27850 arg2Of and,were
R19280 T27852 T27854 arg1Of and,in
R19281 T27853 T27852 arg2Of maintained,and
R19282 T27857 T27854 arg2Of puromycin,in
R19283 T27857 T27855 arg1Of puromycin,10
R19284 T27857 T27856 arg1Of puromycin,µg/ml

GO-CC

Id Subject Object Predicate Lexical cue
T27781 318-323 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27780 40-45 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T27777 348-405 Sentence denotes Cells were selected and maintained in 10 µg/ml puromycin.
T27776 216-347 Sentence denotes Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene.
T27775 0-215 Sentence denotes To generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs).
T362 0-215 Sentence denotes To generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs).
T363 216-347 Sentence denotes Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene.
T364 348-405 Sentence denotes Cells were selected and maintained in 10 µg/ml puromycin.

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T27876 81-98 Entity denotes 2 µg of viral DNA
T27875 386-404 Entity denotes 10 µg/ml puromycin
T27874 31-58 Entity denotes HEK293FT cells (Invitrogen)
T27873 348-353 Entity denotes Cells
T27872 171-214 Entity denotes GenJet transfection reagent (Signagen Labs)
T27870 86-98 Entity denotes of viral DNA
T27869 329-346 Entity denotes 8 µg/ml polybrene
T27868 171-174 Protein denotes Gen

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T27958 404-405 . denotes .
T27957 395-404 NN denotes puromycin
T27956 392-394 NN denotes ml
T27955 391-392 NN denotes /
T27954 389-391 NN denotes µg
T27953 386-388 CD denotes 10
T27952 383-385 IN denotes in
T27951 372-382 VBN denotes maintained
T27950 368-371 CC denotes and
T27949 359-367 VBN denotes selected
T27948 354-358 VBD denotes were
T27947 348-353 NNS denotes Cells
T27946 346-347 . denotes .
T27945 337-346 NN denotes polybrene
T27944 334-336 NN denotes ml
T27943 333-334 NN denotes /
T27942 331-333 NN denotes µg
T27941 329-330 CD denotes 8
T27940 324-328 IN denotes with
T27939 318-323 NNS denotes cells
T27938 313-317 CD denotes L929
T27937 310-312 TO denotes to
T27936 302-309 VBD denotes applied
T27935 298-301 CC denotes and
T27934 291-297 NN denotes filter
T27933 288-290 NN denotes µm
T27932 283-287 CD denotes 0.45
T27931 275-282 IN denotes through
T27930 266-274 VBN denotes filtered
T27929 264-265 , denotes ,
T27928 259-264 RB denotes later
T27927 256-258 NN denotes hr
T27926 253-255 CD denotes 72
T27925 243-252 VBN denotes collected
T27924 239-242 VBD denotes was
T27923 233-238 NNS denotes media
T27922 216-232 VBG denotes Virus-containing
T27921 214-215 . denotes .
T27920 213-214 -RRB- denotes )
T27919 209-213 NNPS denotes Labs
T27918 200-208 NNP denotes Signagen
T27917 199-200 -LRB- denotes (
T27916 191-198 NN denotes reagent
T27915 178-190 NN denotes transfection
T27914 171-177 NNP denotes GenJet
T27913 165-170 VBG denotes using
T27912 158-164 VBZ denotes plates
T27911 153-157 RB denotes well
T27910 151-152 CD denotes 6
T27909 148-150 IN denotes in
T27908 139-147 NNS denotes plasmids
T27907 129-138 JJ denotes accessory
T27906 123-128 NNP denotes VSV-G
T27905 119-122 CC denotes and
T27904 111-118 NN denotes gal/pol
T27903 108-110 IN denotes of
T27902 105-107 NN denotes µg
T27901 103-104 CD denotes 1
T27900 99-102 CC denotes and
T27899 95-98 NN denotes DNA
T27898 89-94 JJ denotes viral
T27897 86-88 IN denotes of
T27896 83-85 NN denotes µg
T27895 81-82 CD denotes 2
T27894 76-80 IN denotes with
T27893 64-75 VBN denotes transfected
T27892 59-63 VBD denotes were
T27891 57-58 -RRB- denotes )
T27890 47-57 NNP denotes Invitrogen
T27889 46-47 -LRB- denotes (
T27888 40-45 NNS denotes cells
T27887 31-39 JJ denotes HEK293FT
T27886 29-30 , denotes ,
T27885 17-29 NNS denotes retroviruses
T27884 12-16 NNP denotes MSCV
T27883 3-11 VB denotes generate
T27882 0-2 TO denotes To
R19298 T27890 T27885 appos Invitrogen,retroviruses
R19299 T27891 T27883 punct ),generate
R19300 T27892 T27893 auxpass were,transfected
R19301 T27893 T27883 dep transfected,generate
R19302 T27894 T27893 prep with,transfected
R19303 T27895 T27896 nummod 2,µg
R19304 T27896 T27894 pobj µg,with
R19305 T27897 T27896 prep of,µg
R19306 T27898 T27899 amod viral,DNA
R19307 T27899 T27897 pobj DNA,of
R19308 T27900 T27896 cc and,µg
R19309 T27901 T27902 nummod 1,µg
R19310 T27902 T27896 conj µg,µg
R19311 T27903 T27902 prep of,µg
R19312 T27904 T27903 pobj gal/pol,of
R19313 T27905 T27904 cc and,gal/pol
R19314 T27906 T27908 compound VSV-G,plasmids
R19315 T27907 T27908 compound accessory,plasmids
R19316 T27908 T27902 conj plasmids,µg
R19317 T27909 T27908 prep in,plasmids
R19318 T27910 T27909 pobj 6,in
R19319 T27911 T27912 intj well,plates
R19320 T27912 T27893 conj plates,transfected
R19321 T27913 T27912 advcl using,plates
R19322 T27914 T27916 nmod GenJet,reagent
R19323 T27915 T27916 compound transfection,reagent
R19324 T27916 T27913 dobj reagent,using
R19325 T27917 T27919 punct (,Labs
R19326 T27918 T27919 compound Signagen,Labs
R19327 T27919 T27916 appos Labs,reagent
R19328 T27920 T27919 punct ),Labs
R19329 T27921 T27883 punct .,generate
R19330 T27922 T27923 amod Virus-containing,media
R19331 T27923 T27925 nsubjpass media,collected
R19332 T27924 T27925 auxpass was,collected
R19333 T27925 T27925 ROOT collected,collected
R19334 T27926 T27928 npadvmod 72,later
R19335 T27927 T27928 npadvmod hr,later
R19336 T27928 T27925 advmod later,collected
R19337 T27929 T27930 punct ",",filtered
R19338 T27930 T27925 advcl filtered,collected
R19339 T27931 T27930 prep through,filtered
R19340 T27932 T27934 nummod 0.45,filter
R19341 T27933 T27934 compound µm,filter
R19342 T27934 T27931 pobj filter,through
R19343 T27935 T27930 cc and,filtered
R19346 T27938 T27939 nummod L929,cells
R19347 T27939 T27937 pobj cells,to
R19348 T27940 T27936 prep with,applied
R19349 T27941 T27942 nummod 8,µg
R19350 T27942 T27945 amod µg,polybrene
R19351 T27943 T27945 nmod /,polybrene
R19352 T27944 T27945 compound ml,polybrene
R19353 T27945 T27940 pobj polybrene,with
R19354 T27946 T27925 punct .,collected
R19355 T27947 T27949 nsubjpass Cells,selected
R19356 T27948 T27949 auxpass were,selected
R19357 T27949 T27949 ROOT selected,selected
R19358 T27950 T27949 cc and,selected
R19359 T27951 T27949 conj maintained,selected
R19360 T27952 T27951 prep in,maintained
R19361 T27953 T27954 nummod 10,µg
R19362 T27954 T27957 compound µg,puromycin
R19363 T27955 T27957 compound /,puromycin
R19364 T27956 T27957 compound ml,puromycin
R19365 T27957 T27952 pobj puromycin,in
R19366 T27958 T27949 punct .,selected
R19290 T27882 T27883 aux To,generate
R19291 T27883 T27883 ROOT generate,generate
R19292 T27884 T27885 compound MSCV,retroviruses
R19293 T27885 T27883 dobj retroviruses,generate
R19294 T27886 T27885 punct ",",retroviruses
R19295 T27887 T27888 amod HEK293FT,cells
R19296 T27888 T27885 conj cells,retroviruses
R19297 T27889 T27890 punct (,Invitrogen
R19344 T27936 T27930 conj applied,filtered
R19345 T27937 T27936 prep to,applied

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T27864 115-118 Protein denotes pol
T27863 111-114 Protein denotes gal