PMC:3585731 / 55366-55431 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T27272	61-65	NNP	denotes	4°C.
T27271	58-60	IN	denotes	at
T27270	48-57	RB	denotes	overnight
T27269	39-47	NN	denotes	BSA/TBST
T27268	38-39	NN	denotes	%
T27267	37-38	CD	denotes	5
T27266	34-36	IN	denotes	in
T27265	24-33	VB	denotes	incubated
T27264	19-23	VB	denotes	were
T27263	8-18	NN	denotes	antibodies
T27262	0-7	JJ	denotes	Primary
R18762	T27263	T27262	arg1Of	antibodies,Primary
R18763	T27263	T27264	arg1Of	antibodies,were
R18764	T27263	T27265	arg2Of	antibodies,incubated
R18765	T27265	T27264	arg2Of	incubated,were
R18766	T27265	T27266	arg1Of	incubated,in
R18767	T27265	T27270	arg1Of	incubated,overnight
R18768	T27267	T27268	arg1Of	5,%
R18769	T27269	T27266	arg2Of	BSA/TBST,in
R18770	T27269	T27267	arg1Of	BSA/TBST,5

Id	Subject	Object	Predicate	Lexical cue
T27081	8-18	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies

Id	Subject	Object	Predicate	Lexical cue
T27094	8-18	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T27091	8-18	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies

Id	Subject	Object	Predicate	Lexical cue
T351	0-65	Sentence	denotes	Primary antibodies were incubated in 5%BSA/TBST overnight at 4°C.

Id	Subject	Object	Predicate	Lexical cue
T27343	0-18	Entity	denotes	Primary antibodies

Id	Subject	Object	Predicate	Lexical cue
T27561	63-65	NNP	denotes	C.
T27560	62-63	CD	denotes	°
T27559	61-62	CD	denotes	4
T27558	58-60	IN	denotes	at
T27557	48-57	JJ	denotes	overnight
T27556	39-47	NNP	denotes	BSA/TBST
T27555	38-39	NN	denotes	%
T27554	37-38	CD	denotes	5
T27553	34-36	IN	denotes	in
T27552	24-33	VBN	denotes	incubated
T27551	19-23	VBD	denotes	were
T27550	8-18	NNS	denotes	antibodies
T27549	0-7	JJ	denotes	Primary
R19001	T27549	T27550	amod	Primary,antibodies
R19002	T27550	T27552	nsubjpass	antibodies,incubated
R19003	T27551	T27552	auxpass	were,incubated
R19004	T27552	T27552	ROOT	incubated,incubated
R19005	T27553	T27552	prep	in,incubated
R19006	T27554	T27555	nummod	5,%
R19007	T27555	T27556	compound	%,BSA/TBST
R19008	T27556	T27557	compound	BSA/TBST,overnight
R19009	T27557	T27553	pobj	overnight,in
R19010	T27558	T27552	prep	at,incubated
R19011	T27559	T27560	nummod	4,°