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PMC:3585731 / 54479-55698 JSONTXT

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testone

Id Subject Object Predicate Lexical cue
T27054 327-331 Protein denotes TNFα
T27053 168-172 Protein denotes TNFα

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T27316 1208-1218 NN denotes antibodies
T27315 1204-1207 JJ denotes new
T27314 1199-1203 IN denotes with
T27313 1190-1198 VB denotes reprobed
T27312 1186-1189 CC denotes and
T27311 1184-1185 -RRB- denotes )
T27310 1173-1184 NNP denotes Biosciences
T27309 1170-1172 NNP denotes GM
T27308 1169-1170 -LRB- denotes (
T27307 1162-1168 NN denotes buffer
T27306 1152-1161 VB denotes stripping
T27305 1142-1151 NN denotes OneMinute
T27304 1136-1141 VB denotes using
T27303 1127-1135 VB denotes stripped
T27302 1122-1126 VB denotes were
T27301 1112-1121 NN denotes membranes
T27300 1110-1111 -COMMA- denotes ,
T27299 1105-1110 NN denotes cases
T27298 1100-1104 DT denotes some
T27297 1097-1099 IN denotes In
T27296 1088-1095 NN denotes signals
T27295 1084-1087 DT denotes the
T27294 1076-1083 VB denotes develop
T27293 1073-1075 TO denotes to
T27292 1068-1072 VB denotes used
T27291 1063-1067 VB denotes were
T27290 1054-1062 NN denotes reagents
T27289 1050-1053 NN denotes ECL
T27288 1048-1049 -RRB- denotes )
T27287 1039-1048 NNP denotes Millipore
T27286 1038-1039 -LRB- denotes (
T27285 1029-1037 NNP denotes Luminata
T27284 1016-1027 NN denotes temperature
T27283 1011-1015 NN denotes room
T27282 1008-1010 IN denotes at
T27281 1004-1007 NN denotes min
T27280 1001-1003 CD denotes 30
T27279 997-1000 IN denotes for
T27278 992-996 NN denotes TBST
T27277 989-991 IN denotes in
T27276 979-988 VB denotes incubated
T27275 974-978 VB denotes were
T27274 963-973 NN denotes antibodies
T27273 953-962 NNP denotes Secondary
T27272 948-952 NNP denotes 4°C.
T27271 945-947 IN denotes at
T27270 935-944 RB denotes overnight
T27269 926-934 NN denotes BSA/TBST
T27268 925-926 NN denotes %
T27267 924-925 CD denotes 5
T27266 921-923 IN denotes in
T27265 911-920 VB denotes incubated
T27264 906-910 VB denotes were
T27263 895-905 NN denotes antibodies
T27262 887-894 JJ denotes Primary
T27261 874-885 NN denotes temperature
T27260 869-873 NN denotes room
T27259 866-868 IN denotes at
T27258 862-865 NN denotes min
T27257 859-861 CD denotes 30
T27256 855-858 IN denotes for
T27255 848-854 NN denotes buffer
T27254 843-847 NN denotes TBST
T27253 840-842 IN denotes in
T27252 838-839 -RRB- denotes )
T27251 835-838 NN denotes BSA
T27250 834-835 -LRB- denotes (
T27249 826-833 NN denotes albumin
T27248 820-825 NN denotes serum
T27247 813-819 JJ denotes bovine
T27246 811-812 NN denotes %
T27245 810-811 CD denotes 5
T27244 807-809 CC denotes or
T27243 802-806 NN denotes milk
T27242 800-801 NN denotes %
T27241 799-800 CD denotes 3
T27240 796-798 IN denotes in
T27239 788-795 VB denotes blocked
T27238 786-787 -COMMA- denotes ,
T27237 778-786 NN denotes membrane
T27236 773-777 NN denotes PVDF
T27235 770-772 TO denotes to
T27234 758-769 VB denotes transferred
T27233 753-757 VB denotes were
T27232 748-752 NN denotes gels
T27231 739-747 NN denotes SDS-PAGE
T27230 737-738 -COMMA- denotes ,
T27229 730-737 RB denotes Briefly
T27228 719-728 NN denotes protocols
T27227 710-718 JJ denotes standard
T27226 707-709 TO denotes to
T27225 697-706 VB denotes according
T27224 687-696 VB denotes performed
T27223 683-686 VB denotes was
T27222 674-682 NN denotes blotting
T27221 666-673 NNP denotes Western
T27220 660-665 NNP denotes 95°C.
T27219 657-659 IN denotes at
T27218 653-656 NN denotes min
T27217 651-652 CD denotes 5
T27216 647-650 IN denotes for
T27215 640-646 VB denotes boiled
T27214 635-639 VB denotes were
T27213 626-634 NN denotes proteins
T27212 623-625 IN denotes of
T27211 615-622 NN denotes amounts
T27210 609-614 JJ denotes Equal
T27209 606-607 -RRB- denotes )
T27208 600-606 NNP denotes Pierce
T27207 599-600 -LRB- denotes (
T27206 591-598 NN denotes Reagent
T27205 585-590 NN denotes Assay
T27204 582-584 NN denotes nm
T27203 578-581 CD denotes 660
T27202 571-577 NNP denotes Pierce
T27201 567-570 DT denotes the
T27200 561-566 VB denotes using
T27199 552-560 VB denotes measured
T27198 547-551 VB denotes were
T27197 532-546 NN denotes concentrations
T27196 524-531 NN denotes Protein
T27195 512-522 NN denotes 14,000×rpm
T27194 509-511 IN denotes at
T27193 505-508 NN denotes min
T27192 502-504 CD denotes 15
T27191 498-501 IN denotes for
T27190 493-497 RP denotes down
T27189 488-492 VB denotes spun
T27188 483-487 VB denotes were
T27187 475-482 NN denotes lysates
T27186 470-474 NN denotes cell
T27185 468-469 -COMMA- denotes ,
T27184 458-468 NN denotes sonication
T27183 452-457 JJ denotes brief
T27182 446-451 IN denotes After
T27181 415-444 NN denotes phenylmethanesulfonylfluoride
T27180 409-414 NN denotes µg/ml
T27179 406-408 CD denotes 50
T27178 401-405 IN denotes with
T27177 388-400 VB denotes supplemented
T27176 386-387 -RRB- denotes )
T27175 377-386 NN denotes Signaling
T27174 372-376 NN denotes Cell
T27173 371-372 -LRB- denotes (
T27172 364-370 NN denotes buffer
T27171 357-363 NN denotes 1×RIPA
T27170 354-356 IN denotes in
T27169 344-353 VB denotes harvested
T27168 339-343 VB denotes were
T27167 333-338 NN denotes Cells
T27166 327-331 NN denotes TNFα
T27165 321-326 NN denotes mouse
T27164 315-320 NN denotes ng/ml
T27163 312-314 CD denotes 10
T27162 309-311 CC denotes or
T27161 300-308 NN denotes zVAD.fmk
T27160 297-299 NN denotes µM
T27159 294-296 CD denotes 20
T27158 292-293 -COMMA- denotes ,
T27157 285-292 NN denotes factors
T27156 278-284 NN denotes growth
T27155 275-277 IN denotes of
T27154 266-274 NN denotes addition
T27153 262-265 DT denotes the
T27152 259-261 TO denotes to
T27151 253-258 JJ denotes prior
T27150 250-252 NN denotes hr
T27149 247-249 CD denotes 24
T27148 243-246 IN denotes for
T27147 235-242 VB denotes starved
T27146 229-234 NN denotes serum
T27145 224-228 VB denotes were
T27144 218-223 NN denotes cells
T27143 216-217 -COMMA- denotes ,
T27142 206-216 NN denotes conditions
T27141 201-205 JJ denotes free
T27140 195-200 NN denotes serum
T27139 189-194 IN denotes under
T27138 178-188 NN denotes treatments
T27137 174-177 IN denotes For
T27136 168-172 NN denotes TNFα
T27135 162-167 NN denotes mouse
T27134 156-161 NN denotes ng/ml
T27133 153-155 CD denotes 10
T27132 150-152 CC denotes or
T27131 141-149 NN denotes zVAD.fmk
T27130 138-140 NN denotes µM
T27123 104-106 NN denotes hr
T27122 98-103 CD denotes 24–48
T27121 92-97 IN denotes After
T27120 84-90 NN denotes dishes
T27119 80-83 NN denotes mm2
T27118 77-79 CD denotes 35
T27117 72-76 IN denotes into
T27116 65-71 VB denotes seeded
T27115 60-64 VB denotes were
T27114 58-59 -RRB- denotes )
T27113 53-58 NN denotes cells
T27112 46-52 NN denotes Jurkat
T27111 40-45 CD denotes 1×106
T27110 39-40 -LRB- denotes (
T27109 33-38 NN denotes cells
T27108 24-32 JJ denotes adherent
T27107 18-23 CD denotes 4×105
T27106 16-17 -COMMA- denotes ,
T27105 12-16 NN denotes blot
T27104 4-11 JJ denotes Western
T27103 0-3 IN denotes For
T27129 135-137 CD denotes 30
T27128 130-134 IN denotes with
T27127 119-129 VB denotes stimulated
T27126 114-118 VB denotes were
T27125 108-113 NN denotes cells
T27124 106-107 -COMMA- denotes ,
R18602 T27105 T27103 arg2Of blot,For
R18603 T27105 T27104 arg1Of blot,Western
R18604 T27109 T27107 arg1Of cells,4×105
R18605 T27109 T27108 arg1Of cells,adherent
R18606 T27109 T27110 arg1Of cells,(
R18607 T27109 T27115 arg1Of cells,were
R18608 T27109 T27116 arg2Of cells,seeded
R18609 T27113 T27110 arg2Of cells,(
R18610 T27113 T27111 arg1Of cells,1×106
R18611 T27113 T27112 arg1Of cells,Jurkat
R18612 T27114 T27110 arg3Of ),(
R18613 T27116 T27103 arg1Of seeded,For
R18614 T27116 T27106 arg1Of seeded,","
R18615 T27116 T27115 arg2Of seeded,were
R18616 T27116 T27117 arg1Of seeded,into
R18617 T27120 T27117 arg2Of dishes,into
R18618 T27120 T27118 arg1Of dishes,35
R18619 T27120 T27119 arg1Of dishes,mm2
R18620 T27123 T27121 arg2Of hr,After
R18621 T27123 T27122 arg1Of hr,24–48
R18622 T27125 T27126 arg1Of cells,were
R18623 T27125 T27127 arg2Of cells,stimulated
R18624 T27127 T27121 arg1Of stimulated,After
R18625 T27127 T27124 arg1Of stimulated,","
R18626 T27127 T27126 arg2Of stimulated,were
R18627 T27127 T27128 arg1Of stimulated,with
R18628 T27129 T27130 arg1Of 30,µM
R18629 T27131 T27129 arg1Of zVAD.fmk,30
R18630 T27131 T27132 arg1Of zVAD.fmk,or
R18631 T27132 T27128 arg2Of or,with
R18632 T27136 T27132 arg2Of TNFα,or
R18633 T27136 T27133 arg1Of TNFα,10
R18634 T27136 T27134 arg1Of TNFα,ng/ml
R18635 T27136 T27135 arg1Of TNFα,mouse
R18636 T27138 T27137 arg2Of treatments,For
R18637 T27138 T27139 arg1Of treatments,under
R18638 T27142 T27139 arg2Of conditions,under
R18639 T27142 T27140 arg1Of conditions,serum
R18640 T27142 T27141 arg1Of conditions,free
R18641 T27144 T27145 arg1Of cells,were
R18642 T27145 T27137 arg1Of were,For
R18643 T27145 T27143 arg1Of were,","
R18644 T27146 T27145 arg2Of serum,were
R18645 T27146 T27147 arg2Of serum,starved
R18646 T27147 T27148 arg1Of starved,for
R18647 T27150 T27148 arg2Of hr,for
R18648 T27150 T27149 arg1Of hr,24
R18649 T27150 T27151 arg1Of hr,prior
R18650 T27151 T27152 arg1Of prior,to
R18651 T27154 T27152 arg2Of addition,to
R18652 T27154 T27153 arg1Of addition,the
R18653 T27154 T27155 arg1Of addition,of
R18654 T27154 T27158 arg1Of addition,","
R18655 T27157 T27155 arg2Of factors,of
R18656 T27157 T27156 arg1Of factors,growth
R18657 T27159 T27160 arg1Of 20,µM
R18658 T27161 T27159 arg1Of zVAD.fmk,20
R18659 T27161 T27162 arg1Of zVAD.fmk,or
R18660 T27162 T27158 arg2Of or,","
R18661 T27166 T27162 arg2Of TNFα,or
R18662 T27166 T27163 arg1Of TNFα,10
R18663 T27166 T27164 arg1Of TNFα,ng/ml
R18664 T27166 T27165 arg1Of TNFα,mouse
R18665 T27167 T27168 arg1Of Cells,were
R18666 T27167 T27169 arg2Of Cells,harvested
R18667 T27169 T27168 arg2Of harvested,were
R18668 T27169 T27170 arg1Of harvested,in
R18669 T27172 T27170 arg2Of buffer,in
R18670 T27172 T27171 arg1Of buffer,1×RIPA
R18671 T27172 T27173 arg1Of buffer,(
R18672 T27172 T27177 arg2Of buffer,supplemented
R18673 T27175 T27173 arg2Of Signaling,(
R18674 T27175 T27174 arg1Of Signaling,Cell
R18675 T27176 T27173 arg3Of ),(
R18676 T27177 T27178 arg1Of supplemented,with
R18677 T27181 T27178 arg2Of phenylmethanesulfonylfluoride,with
R18678 T27181 T27179 arg1Of phenylmethanesulfonylfluoride,50
R18679 T27181 T27180 arg1Of phenylmethanesulfonylfluoride,µg/ml
R18680 T27184 T27182 arg2Of sonication,After
R18681 T27184 T27183 arg1Of sonication,brief
R18682 T27187 T27186 arg1Of lysates,cell
R18683 T27187 T27188 arg1Of lysates,were
R18684 T27187 T27189 arg2Of lysates,spun
R18685 T27189 T27182 arg1Of spun,After
R18686 T27189 T27185 arg1Of spun,","
R18687 T27189 T27188 arg2Of spun,were
R18688 T27189 T27190 arg1Of spun,down
R18689 T27189 T27191 arg1Of spun,for
R18690 T27189 T27194 arg1Of spun,at
R18691 T27193 T27191 arg2Of min,for
R18692 T27193 T27192 arg1Of min,15
R18693 T27195 T27194 arg2Of "14,000×rpm",at
R18694 T27197 T27196 arg1Of concentrations,Protein
R18695 T27197 T27198 arg1Of concentrations,were
R18696 T27197 T27199 arg2Of concentrations,measured
R18697 T27199 T27198 arg2Of measured,were
R18698 T27200 T27199 arg3Of using,measured
R18699 T27200 T27207 arg1Of using,(
R18700 T27202 T27203 arg1Of Pierce,660
R18701 T27206 T27200 arg2Of Reagent,using
R18702 T27206 T27201 arg1Of Reagent,the
R18703 T27206 T27202 arg1Of Reagent,Pierce
R18704 T27206 T27204 arg1Of Reagent,nm
R18705 T27206 T27205 arg1Of Reagent,Assay
R18706 T27208 T27207 arg2Of Pierce,(
R18707 T27209 T27207 arg3Of ),(
R18708 T27211 T27210 arg1Of amounts,Equal
R18709 T27211 T27212 arg1Of amounts,of
R18710 T27211 T27214 arg1Of amounts,were
R18711 T27211 T27215 arg2Of amounts,boiled
R18712 T27213 T27212 arg2Of proteins,of
R18713 T27215 T27214 arg2Of boiled,were
R18714 T27215 T27216 arg1Of boiled,for
R18715 T27218 T27216 arg2Of min,for
R18716 T27218 T27217 arg1Of min,5
R18717 T27218 T27219 arg1Of min,at
R18718 T27221 T27219 arg2Of Western,at
R18719 T27221 T27220 arg1Of Western,95°C.
R18720 T27222 T27223 arg1Of blotting,was
R18721 T27222 T27224 arg2Of blotting,performed
R18722 T27224 T27214 modOf performed,were
R18723 T27224 T27223 arg2Of performed,was
R18724 T27224 T27225 arg1Of performed,according
R18725 T27226 T27225 arg2Of to,according
R18726 T27228 T27226 arg2Of protocols,to
R18727 T27228 T27227 arg1Of protocols,standard
R18728 T27232 T27231 arg1Of gels,SDS-PAGE
R18729 T27232 T27233 arg1Of gels,were
R18730 T27232 T27234 arg2Of gels,transferred
R18731 T27232 T27239 arg1Of gels,blocked
R18732 T27234 T27233 arg2Of transferred,were
R18733 T27234 T27235 arg1Of transferred,to
R18734 T27234 T27238 arg1Of transferred,","
R18735 T27237 T27235 arg2Of membrane,to
R18736 T27237 T27236 arg1Of membrane,PVDF
R18737 T27238 T27229 arg1Of ",",Briefly
R18738 T27238 T27230 arg1Of ",",","
R18739 T27238 T27256 arg1Of ",",for
R18740 T27239 T27238 arg2Of blocked,","
R18741 T27239 T27240 arg1Of blocked,in
R18742 T27242 T27240 arg2Of %,in
R18743 T27242 T27241 arg1Of %,3
R18744 T27243 T27244 arg1Of milk,or
R18745 T27244 T27239 arg2Of or,blocked
R18746 T27245 T27246 arg1Of 5,%
R18747 T27249 T27244 arg2Of albumin,or
R18748 T27249 T27245 arg1Of albumin,5
R18749 T27249 T27247 arg1Of albumin,bovine
R18750 T27249 T27248 arg1Of albumin,serum
R18751 T27249 T27250 arg1Of albumin,(
R18752 T27249 T27253 arg1Of albumin,in
R18753 T27251 T27250 arg2Of BSA,(
R18754 T27252 T27250 arg3Of ),(
R18755 T27255 T27253 arg2Of buffer,in
R18756 T27255 T27254 arg1Of buffer,TBST
R18757 T27258 T27256 arg2Of min,for
R18758 T27258 T27257 arg1Of min,30
R18759 T27258 T27259 arg1Of min,at
R18760 T27261 T27259 arg2Of temperature,at
R18761 T27261 T27260 arg1Of temperature,room
R18762 T27263 T27262 arg1Of antibodies,Primary
R18763 T27263 T27264 arg1Of antibodies,were
R18764 T27263 T27265 arg2Of antibodies,incubated
R18765 T27265 T27264 arg2Of incubated,were
R18766 T27265 T27266 arg1Of incubated,in
R18767 T27265 T27270 arg1Of incubated,overnight
R18768 T27267 T27268 arg1Of 5,%
R18769 T27269 T27266 arg2Of BSA/TBST,in
R18770 T27269 T27267 arg1Of BSA/TBST,5
R18771 T27270 T27275 modOf overnight,were
R18772 T27273 T27272 arg1Of Secondary,4°C.
R18773 T27274 T27273 arg1Of antibodies,Secondary
R18774 T27274 T27275 arg1Of antibodies,were
R18775 T27274 T27276 arg2Of antibodies,incubated
R18776 T27276 T27271 arg1Of incubated,at
R18777 T27276 T27275 arg2Of incubated,were
R18778 T27276 T27277 arg1Of incubated,in
R18779 T27276 T27279 arg1Of incubated,for
R18780 T27276 T27282 arg1Of incubated,at
R18781 T27278 T27277 arg2Of TBST,in
R18782 T27281 T27279 arg2Of min,for
R18783 T27281 T27280 arg1Of min,30
R18784 T27284 T27282 arg2Of temperature,at
R18785 T27284 T27283 arg1Of temperature,room
R18786 T27287 T27286 arg2Of Millipore,(
R18787 T27288 T27286 arg3Of ),(
R18788 T27290 T27285 arg1Of reagents,Luminata
R18789 T27290 T27286 arg1Of reagents,(
R18790 T27290 T27289 arg1Of reagents,ECL
R18791 T27290 T27291 arg1Of reagents,were
R18792 T27290 T27292 arg2Of reagents,used
R18793 T27292 T27291 arg2Of used,were
R18794 T27294 T27292 arg3Of develop,used
R18795 T27294 T27293 arg1Of develop,to
R18796 T27296 T27294 arg2Of signals,develop
R18797 T27296 T27295 arg1Of signals,the
R18798 T27299 T27297 arg2Of cases,In
R18799 T27299 T27298 arg1Of cases,some
R18800 T27301 T27302 arg1Of membranes,were
R18801 T27301 T27303 arg2Of membranes,stripped
R18802 T27301 T27313 arg2Of membranes,reprobed
R18803 T27303 T27312 arg1Of stripped,and
R18804 T27304 T27303 arg3Of using,stripped
R18805 T27305 T27304 arg2Of OneMinute,using
R18806 T27305 T27306 arg1Of OneMinute,stripping
R18807 T27307 T27306 arg2Of buffer,stripping
R18808 T27307 T27308 arg1Of buffer,(
R18809 T27310 T27308 arg2Of Biosciences,(
R18810 T27310 T27309 arg1Of Biosciences,GM
R18811 T27311 T27308 arg3Of ),(
R18812 T27312 T27297 arg1Of and,In
R18813 T27312 T27300 arg1Of and,","
R18814 T27312 T27302 arg2Of and,were
R18815 T27313 T27312 arg2Of reprobed,and
R18816 T27313 T27314 arg1Of reprobed,with
R18817 T27316 T27314 arg2Of antibodies,with
R18818 T27316 T27315 arg1Of antibodies,new

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T27078 327-331 Protein denotes TNFα
T27077 168-172 Protein denotes TNFα

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T27320 327-331 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T27319 168-172 http://www.uniprot.org/uniprot/P01375 denotes TNFα

UBERON-AE

Id Subject Object Predicate Lexical cue
T27062 802-806 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T27061 820-825 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T27060 229-234 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T27059 195-200 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T27080 1088-1095 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T27079 278-284 http://purl.obolibrary.org/obo/GO_0040007 denotes growth

GO-MF

Id Subject Object Predicate Lexical cue
T27083 1208-1218 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27082 963-973 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27081 895-905 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T27096 1208-1218 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27095 963-973 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27094 895-905 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27093 1208-1218 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27092 963-973 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27091 895-905 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27090 1112-1121 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T27089 778-786 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T27088 470-474 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T27087 218-223 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27086 108-113 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27085 53-58 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27084 33-38 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T27074 1097-1219 Sentence denotes In some cases, membranes were stripped using OneMinute stripping buffer (GM Biosciences) and reprobed with new antibodies.
T27073 1029-1096 Sentence denotes Luminata (Millipore) ECL reagents were used to develop the signals.
T27072 887-1028 Sentence denotes Primary antibodies were incubated in 5%BSA/TBST overnight at 4°C. Secondary antibodies were incubated in TBST for 30 min at room temperature.
T27071 730-886 Sentence denotes Briefly, SDS-PAGE gels were transferred to PVDF membrane, blocked in 3% milk or 5% bovine serum albumin (BSA) in TBST buffer for 30 min at room temperature.
T27070 609-729 Sentence denotes Equal amounts of proteins were boiled for 5 min at 95°C. Western blotting was performed according to standard protocols.
T27069 524-608 Sentence denotes Protein concentrations were measured using the Pierce 660 nm Assay Reagent (Pierce).
T27068 446-523 Sentence denotes After brief sonication, cell lysates were spun down for 15 min at 14,000×rpm.
T27067 333-445 Sentence denotes Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.
T27066 174-332 Sentence denotes For treatments under serum free conditions, cells were serum starved for 24 hr prior to the addition of growth factors, 20 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T27065 92-173 Sentence denotes After 24–48 hr, cells were stimulated with 30 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T27064 0-91 Sentence denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes.
T342 0-91 Sentence denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes.
T343 92-173 Sentence denotes After 24–48 hr, cells were stimulated with 30 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T344 174-332 Sentence denotes For treatments under serum free conditions, cells were serum starved for 24 hr prior to the addition of growth factors, 20 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T345 333-445 Sentence denotes Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.
T346 446-523 Sentence denotes After brief sonication, cell lysates were spun down for 15 min at 14,000×rpm.
T347 524-608 Sentence denotes Protein concentrations were measured using the Pierce 660 nm Assay Reagent (Pierce).
T348 609-665 Sentence denotes Equal amounts of proteins were boiled for 5 min at 95°C.
T349 666-729 Sentence denotes Western blotting was performed according to standard protocols.
T350 730-886 Sentence denotes Briefly, SDS-PAGE gels were transferred to PVDF membrane, blocked in 3% milk or 5% bovine serum albumin (BSA) in TBST buffer for 30 min at room temperature.
T351 887-952 Sentence denotes Primary antibodies were incubated in 5%BSA/TBST overnight at 4°C.
T352 953-1028 Sentence denotes Secondary antibodies were incubated in TBST for 30 min at room temperature.
T353 1029-1096 Sentence denotes Luminata (Millipore) ECL reagents were used to develop the signals.
T354 1097-1219 Sentence denotes In some cases, membranes were stripped using OneMinute stripping buffer (GM Biosciences) and reprobed with new antibodies.

simple1

Id Subject Object Predicate Lexical cue
T27100 327-331 Protein denotes TNFα
T27099 168-172 Protein denotes TNFα

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T27318 327-331 Protein denotes TNFα
T27317 168-172 Protein denotes TNFα

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T27326 327-331 Protein denotes TNFα
T27325 168-172 Protein denotes TNFα

DLUT931

Id Subject Object Predicate Lexical cue
T27322 327-331 Protein denotes TNFα
T27321 168-172 Protein denotes TNFα

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T27345 40-58 Entity denotes 1×106 Jurkat cells
T27344 524-546 Entity denotes Protein concentrations
T27343 887-905 Entity denotes Primary antibodies
T27342 135-149 Entity denotes 30 µM zVAD.fmk
T27341 773-777 Entity denotes PVDF
T27340 810-839 Protein denotes 5% bovine serum albumin (BSA)
T27339 623-634 Protein denotes of proteins
T27366 333-363 Binding denotes Cells were harvested in 1×RIPA
T27365 0-90 Binding denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes
T27364 582-607 Entity denotes nm Assay Reagent (Pierce)
T27363 218-223 Entity denotes cells
T27362 1050-1062 Entity denotes ECL reagents
T27361 773-786 Entity denotes PVDF membrane
T27360 953-973 Entity denotes Secondary antibodies
T27359 18-59 Entity denotes 4×105 adherent cells (1×106 Jurkat cells)
T27358 108-113 Entity denotes cells
T27357 1170-1172 Entity denotes GM
T27356 333-338 Entity denotes Cells
T27355 1204-1218 Entity denotes new antibodies
T27354 372-376 Entity denotes Cell
T27353 300-308 Entity denotes zVAD.fmk
T27352 470-482 Entity denotes cell lysates
T27351 1050-1053 Protein denotes ECL
T27350 1112-1121 Entity denotes membranes
T27349 153-172 Protein denotes 10 ng/ml mouse TNFα
T27348 294-331 Protein denotes 20 µM zVAD.fmk or 10 ng/ml mouse TNFα
T27347 739-752 Entity denotes SDS-PAGE gels
T27346 835-838 Protein denotes BSA

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T27575 1029-1037 NNP denotes Luminata
T27608 1218-1219 . denotes .
T27607 1208-1218 NNS denotes antibodies
T27606 1204-1207 JJ denotes new
T27605 1199-1203 IN denotes with
T27604 1190-1198 VBN denotes reprobed
T27603 1186-1189 CC denotes and
T27602 1184-1185 -RRB- denotes )
T27601 1173-1184 NNPS denotes Biosciences
T27600 1170-1172 NNP denotes GM
T27599 1169-1170 -LRB- denotes (
T27598 1162-1168 NN denotes buffer
T27597 1152-1161 VBG denotes stripping
T27596 1142-1151 NNP denotes OneMinute
T27595 1136-1141 VBG denotes using
T27594 1127-1135 VBN denotes stripped
T27593 1122-1126 VBD denotes were
T27592 1112-1121 NNS denotes membranes
T27591 1110-1111 , denotes ,
T27590 1105-1110 NNS denotes cases
T27589 1100-1104 DT denotes some
T27588 1097-1099 IN denotes In
T27587 1095-1096 . denotes .
T27586 1088-1095 NNS denotes signals
T27585 1084-1087 DT denotes the
T27584 1076-1083 VB denotes develop
T27583 1073-1075 TO denotes to
T27582 1068-1072 VBN denotes used
T27581 1063-1067 VBD denotes were
T27580 1054-1062 NNS denotes reagents
T27579 1050-1053 NNP denotes ECL
T27578 1048-1049 -RRB- denotes )
T27577 1039-1048 NNP denotes Millipore
T27576 1038-1039 -LRB- denotes (
T27574 1027-1028 . denotes .
T27573 1016-1027 NN denotes temperature
T27572 1011-1015 NN denotes room
T27571 1008-1010 IN denotes at
T27570 1004-1007 NN denotes min
T27569 1001-1003 CD denotes 30
T27568 997-1000 IN denotes for
T27567 992-996 NNP denotes TBST
T27566 989-991 IN denotes in
T27565 979-988 VBN denotes incubated
T27564 974-978 VBD denotes were
T27563 963-973 NNS denotes antibodies
T27562 953-962 JJ denotes Secondary
T27561 950-952 NNP denotes C.
T27560 949-950 CD denotes °
T27559 948-949 CD denotes 4
T27558 945-947 IN denotes at
T27557 935-944 JJ denotes overnight
T27556 926-934 NNP denotes BSA/TBST
T27555 925-926 NN denotes %
T27554 924-925 CD denotes 5
T27553 921-923 IN denotes in
T27552 911-920 VBN denotes incubated
T27551 906-910 VBD denotes were
T27550 895-905 NNS denotes antibodies
T27549 887-894 JJ denotes Primary
T27548 885-886 . denotes .
T27547 874-885 NN denotes temperature
T27546 869-873 NN denotes room
T27545 866-868 IN denotes at
T27544 862-865 NN denotes min
T27543 859-861 CD denotes 30
T27542 855-858 IN denotes for
T27541 848-854 NN denotes buffer
T27540 843-847 NNP denotes TBST
T27539 840-842 IN denotes in
T27538 838-839 -RRB- denotes )
T27537 835-838 NNP denotes BSA
T27536 834-835 -LRB- denotes (
T27535 826-833 NN denotes albumin
T27534 820-825 NN denotes serum
T27533 813-819 JJ denotes bovine
T27532 811-812 NN denotes %
T27531 810-811 CD denotes 5
T27530 807-809 CC denotes or
T27529 802-806 NN denotes milk
T27528 800-801 NN denotes %
T27527 799-800 CD denotes 3
T27526 796-798 IN denotes in
T27525 788-795 VBN denotes blocked
T27524 786-787 , denotes ,
T27523 778-786 NN denotes membrane
T27522 773-777 NNP denotes PVDF
T27521 770-772 TO denotes to
T27520 758-769 VBN denotes transferred
T27519 753-757 VBD denotes were
T27518 748-752 NNS denotes gels
T27517 739-747 NNP denotes SDS-PAGE
T27516 737-738 , denotes ,
T27515 730-737 RB denotes Briefly
T27514 728-729 . denotes .
T27513 719-728 NNS denotes protocols
T27512 710-718 JJ denotes standard
T27511 707-709 TO denotes to
T27510 697-706 VBG denotes according
T27509 687-696 VBN denotes performed
T27508 683-686 VBD denotes was
T27507 674-682 VBG denotes blotting
T27506 666-673 NNP denotes Western
T27505 663-665 NNP denotes C.
T27504 662-663 CD denotes °
T27503 660-662 CD denotes 95
T27502 657-659 IN denotes at
T27501 653-656 NN denotes min
T27500 651-652 CD denotes 5
T27499 647-650 IN denotes for
T27498 640-646 VBN denotes boiled
T27497 635-639 VBD denotes were
T27496 626-634 NNS denotes proteins
T27495 623-625 IN denotes of
T27494 615-622 NNS denotes amounts
T27493 609-614 NNP denotes Equal
T27492 607-608 . denotes .
T27491 606-607 -RRB- denotes )
T27490 600-606 NNP denotes Pierce
T27489 599-600 -LRB- denotes (
T27488 591-598 NNP denotes Reagent
T27487 585-590 NNP denotes Assay
T27486 582-584 NN denotes nm
T27485 578-581 CD denotes 660
T27484 571-577 NNP denotes Pierce
T27483 567-570 DT denotes the
T27482 561-566 VBG denotes using
T27481 552-560 VBN denotes measured
T27480 547-551 VBD denotes were
T27479 532-546 NNS denotes concentrations
T27478 524-531 NN denotes Protein
T27477 522-523 . denotes .
T27476 519-522 NN denotes rpm
T27475 518-519 CD denotes ×
T27474 512-518 CD denotes 14,000
T27473 509-511 IN denotes at
T27472 505-508 NN denotes min
T27471 502-504 CD denotes 15
T27470 498-501 IN denotes for
T27469 493-497 RP denotes down
T27468 488-492 VBN denotes spun
T27467 483-487 VBD denotes were
T27466 475-482 NNS denotes lysates
T27465 470-474 NN denotes cell
T27464 468-469 , denotes ,
T27463 458-468 NN denotes sonication
T27462 452-457 JJ denotes brief
T27461 446-451 IN denotes After
T27460 444-445 . denotes .
T27459 415-444 NN denotes phenylmethanesulfonylfluoride
T27458 412-414 NN denotes ml
T27457 411-412 NN denotes /
T27456 409-411 NN denotes µg
T27455 406-408 CD denotes 50
T27454 401-405 IN denotes with
T27453 388-400 VBN denotes supplemented
T27452 386-387 -RRB- denotes )
T27451 377-386 NNP denotes Signaling
T27450 372-376 NNP denotes Cell
T27449 371-372 -LRB- denotes (
T27448 364-370 NN denotes buffer
T27447 359-363 NNP denotes RIPA
T27446 358-359 CD denotes ×
T27445 357-358 CD denotes 1
T27444 354-356 IN denotes in
T27443 344-353 VBN denotes harvested
T27442 339-343 VBD denotes were
T27441 333-338 NNS denotes Cells
T27440 331-332 . denotes .
T27439 327-331 NNP denotes TNFα
T27438 321-326 NN denotes mouse
T27437 315-320 JJ denotes ng/ml
T27436 312-314 CD denotes 10
T27435 309-311 CC denotes or
T27434 300-308 NNP denotes zVAD.fmk
T27433 297-299 NNP denotes µM
T27432 294-296 CD denotes 20
T27431 292-293 , denotes ,
T27430 285-292 NNS denotes factors
T27429 278-284 NN denotes growth
T27428 275-277 IN denotes of
T27427 266-274 NN denotes addition
T27426 262-265 DT denotes the
T27425 259-261 TO denotes to
T27424 253-258 RB denotes prior
T27423 250-252 NN denotes hr
T27422 247-249 CD denotes 24
T27421 243-246 IN denotes for
T27420 235-242 VBN denotes starved
T27419 229-234 JJ denotes serum
T27418 224-228 VBD denotes were
T27417 218-223 NNS denotes cells
T27416 216-217 , denotes ,
T27415 206-216 NNS denotes conditions
T27414 201-205 JJ denotes free
T27413 195-200 NN denotes serum
T27412 189-194 IN denotes under
T27411 178-188 NNS denotes treatments
T27410 174-177 IN denotes For
T27409 172-173 . denotes .
T27408 168-172 NNP denotes TNFα
T27407 162-167 NN denotes mouse
T27406 156-161 JJ denotes ng/ml
T27405 153-155 CD denotes 10
T27404 150-152 CC denotes or
T27403 141-149 NNP denotes zVAD.fmk
T27402 138-140 NNP denotes µM
T27401 135-137 CD denotes 30
T27400 130-134 IN denotes with
T27399 119-129 VBN denotes stimulated
T27398 114-118 VBD denotes were
T27397 108-113 NNS denotes cells
T27396 106-107 , denotes ,
T27395 104-106 NN denotes hr
T27394 101-103 CD denotes 48
T27393 98-100 CD denotes 24
T27392 92-97 IN denotes After
T27391 90-91 . denotes .
T27390 84-90 NNS denotes dishes
T27389 80-83 CD denotes mm2
T27388 77-79 CD denotes 35
T27387 72-76 IN denotes into
T27386 65-71 VBN denotes seeded
T27385 60-64 VBD denotes were
T27384 58-59 -RRB- denotes )
T27383 53-58 NNS denotes cells
T27382 46-52 NN denotes Jurkat
T27381 42-45 CD denotes 106
T27380 41-42 CD denotes ×
T27379 40-41 CD denotes 1
T27378 39-40 -LRB- denotes (
T27377 33-38 NNS denotes cells
T27376 24-32 JJ denotes adherent
T27375 20-23 CD denotes 105
T27374 19-20 CD denotes ×
T27373 18-19 CD denotes 4
T27372 16-17 , denotes ,
T27371 12-16 NN denotes blot
T27370 4-11 JJ denotes Western
T27369 0-3 IN denotes For
R18821 T27369 T27386 prep For,seeded
R18822 T27370 T27371 amod Western,blot
R18823 T27371 T27369 pobj blot,For
R18824 T27372 T27386 punct ",",seeded
R18825 T27373 T27377 nummod 4,cells
R18826 T27374 T27377 nummod ×,cells
R18827 T27375 T27377 nummod 105,cells
R18828 T27376 T27377 amod adherent,cells
R18829 T27377 T27386 nsubjpass cells,seeded
R18830 T27378 T27377 punct (,cells
R18831 T27379 T27383 nummod 1,cells
R18832 T27380 T27383 nummod ×,cells
R18833 T27381 T27383 nummod 106,cells
R18834 T27382 T27383 compound Jurkat,cells
R18835 T27383 T27377 appos cells,cells
R18836 T27384 T27377 punct ),cells
R18837 T27385 T27386 auxpass were,seeded
R18838 T27386 T27386 ROOT seeded,seeded
R18839 T27387 T27386 prep into,seeded
R18840 T27388 T27390 nummod 35,dishes
R18841 T27389 T27390 nummod mm2,dishes
R18842 T27390 T27387 pobj dishes,into
R18843 T27391 T27386 punct .,seeded
R18844 T27392 T27399 prep After,stimulated
R18845 T27393 T27392 pobj 24,After
R18846 T27394 T27395 nummod 48,hr
R18847 T27395 T27393 appos hr,24
R18848 T27396 T27399 punct ",",stimulated
R18849 T27397 T27399 nsubjpass cells,stimulated
R18850 T27398 T27399 auxpass were,stimulated
R18851 T27399 T27399 ROOT stimulated,stimulated
R18852 T27400 T27399 prep with,stimulated
R18853 T27401 T27403 nummod 30,zVAD.fmk
R18854 T27402 T27403 compound µM,zVAD.fmk
R18855 T27403 T27400 pobj zVAD.fmk,with
R18856 T27404 T27403 cc or,zVAD.fmk
R18857 T27405 T27407 nummod 10,mouse
R18858 T27406 T27407 amod ng/ml,mouse
R18859 T27407 T27408 compound mouse,TNFα
R18860 T27408 T27403 conj TNFα,zVAD.fmk
R18861 T27409 T27399 punct .,stimulated
R18862 T27410 T27420 prep For,starved
R18863 T27411 T27410 pobj treatments,For
R18864 T27412 T27411 prep under,treatments
R18865 T27413 T27415 nmod serum,conditions
R18866 T27414 T27415 amod free,conditions
R18867 T27415 T27412 pobj conditions,under
R18868 T27416 T27420 punct ",",starved
R18869 T27417 T27420 nsubjpass cells,starved
R18870 T27418 T27420 auxpass were,starved
R18871 T27419 T27420 advmod serum,starved
R18872 T27420 T27420 ROOT starved,starved
R18873 T27421 T27420 prep for,starved
R18874 T27422 T27423 nummod 24,hr
R18875 T27423 T27421 pobj hr,for
R18876 T27424 T27420 advmod prior,starved
R18877 T27425 T27424 prep to,prior
R18878 T27426 T27427 det the,addition
R18879 T27427 T27425 pobj addition,to
R18880 T27428 T27427 prep of,addition
R18881 T27429 T27430 compound growth,factors
R18882 T27430 T27428 pobj factors,of
R18883 T27431 T27430 punct ",",factors
R18884 T27432 T27434 nummod 20,zVAD.fmk
R18885 T27433 T27434 compound µM,zVAD.fmk
R18886 T27434 T27430 appos zVAD.fmk,factors
R18887 T27435 T27434 cc or,zVAD.fmk
R18888 T27436 T27439 nummod 10,TNFα
R18889 T27437 T27438 amod ng/ml,mouse
R18890 T27438 T27439 compound mouse,TNFα
R18891 T27439 T27434 conj TNFα,zVAD.fmk
R18892 T27440 T27420 punct .,starved
R18893 T27441 T27443 nsubjpass Cells,harvested
R18894 T27442 T27443 auxpass were,harvested
R18895 T27443 T27443 ROOT harvested,harvested
R18896 T27444 T27443 prep in,harvested
R18897 T27445 T27446 nummod 1,×
R18898 T27446 T27448 nummod ×,buffer
R18899 T27447 T27448 compound RIPA,buffer
R18900 T27448 T27444 pobj buffer,in
R18901 T27449 T27448 punct (,buffer
R18902 T27450 T27451 compound Cell,Signaling
R18903 T27451 T27448 appos Signaling,buffer
R18904 T27452 T27448 punct ),buffer
R18905 T27453 T27443 conj supplemented,harvested
R18906 T27454 T27453 prep with,supplemented
R18907 T27455 T27456 nummod 50,µg
R18908 T27456 T27459 nmod µg,phenylmethanesulfonylfluoride
R18909 T27457 T27459 nmod /,phenylmethanesulfonylfluoride
R18910 T27458 T27459 compound ml,phenylmethanesulfonylfluoride
R18911 T27459 T27454 pobj phenylmethanesulfonylfluoride,with
R18912 T27460 T27443 punct .,harvested
R18913 T27461 T27468 prep After,spun
R18914 T27462 T27463 amod brief,sonication
R18915 T27463 T27468 advcl sonication,spun
R18916 T27464 T27468 punct ",",spun
R18917 T27465 T27466 compound cell,lysates
R18918 T27466 T27468 nsubjpass lysates,spun
R18919 T27467 T27468 auxpass were,spun
R18920 T27468 T27468 ROOT spun,spun
R18921 T27469 T27468 prt down,spun
R18922 T27470 T27468 prep for,spun
R18923 T27471 T27472 nummod 15,min
R18924 T27472 T27470 pobj min,for
R18925 T27473 T27472 prep at,min
R18926 T27474 T27475 nummod "14,000",×
R18927 T27475 T27476 nummod ×,rpm
R18928 T27476 T27473 pobj rpm,at
R18929 T27477 T27468 punct .,spun
R18930 T27478 T27479 compound Protein,concentrations
R18931 T27479 T27481 nsubjpass concentrations,measured
R18932 T27480 T27481 auxpass were,measured
R18933 T27481 T27481 ROOT measured,measured
R18934 T27482 T27481 advcl using,measured
R18935 T27483 T27484 det the,Pierce
R18936 T27484 T27482 dobj Pierce,using
R18937 T27485 T27484 nummod 660,Pierce
R18938 T27486 T27488 compound nm,Reagent
R18939 T27487 T27488 compound Assay,Reagent
R18940 T27488 T27484 conj Reagent,Pierce
R18941 T27489 T27490 punct (,Pierce
R18942 T27490 T27488 appos Pierce,Reagent
R18943 T27491 T27488 punct ),Reagent
R18944 T27492 T27481 punct .,measured
R18945 T27493 T27494 compound Equal,amounts
R18946 T27494 T27498 nsubjpass amounts,boiled
R18947 T27495 T27494 prep of,amounts
R18948 T27496 T27495 pobj proteins,of
R18949 T27497 T27498 auxpass were,boiled
R18950 T27498 T27498 ROOT boiled,boiled
R18951 T27499 T27498 prep for,boiled
R18952 T27500 T27501 nummod 5,min
R18953 T27501 T27499 pobj min,for
R18954 T27502 T27498 prep at,boiled
R18955 T27503 T27504 nummod 95,°
R18956 T27504 T27507 nummod °,blotting
R18957 T27505 T27506 compound C.,Western
R18958 T27506 T27507 compound Western,blotting
R18959 T27507 T27502 pobj blotting,at
R18960 T27508 T27509 auxpass was,performed
R18961 T27509 T27498 conj performed,boiled
R18962 T27510 T27509 prep according,performed
R18963 T27511 T27510 prep to,according
R18964 T27512 T27513 amod standard,protocols
R18965 T27513 T27511 pobj protocols,to
R18966 T27514 T27498 punct .,boiled
R18967 T27515 T27520 advmod Briefly,transferred
R18968 T27516 T27520 punct ",",transferred
R18969 T27517 T27518 compound SDS-PAGE,gels
R18970 T27518 T27520 nsubjpass gels,transferred
R18971 T27519 T27520 auxpass were,transferred
R18972 T27520 T27520 ROOT transferred,transferred
R18973 T27521 T27520 prep to,transferred
R18974 T27522 T27523 compound PVDF,membrane
R18975 T27523 T27521 pobj membrane,to
R18976 T27524 T27523 punct ",",membrane
R18977 T27525 T27520 conj blocked,transferred
R18978 T27526 T27525 prep in,blocked
R18979 T27527 T27528 nummod 3,%
R18980 T27528 T27529 compound %,milk
R18981 T27529 T27526 pobj milk,in
R18983 T27531 T27532 nummod 5,%
R18984 T27532 T27535 compound %,albumin
R18985 T27533 T27534 amod bovine,serum
R18986 T27534 T27535 compound serum,albumin
R18987 T27535 T27529 conj albumin,milk
R18988 T27536 T27537 punct (,BSA
R18989 T27537 T27535 appos BSA,albumin
R18990 T27538 T27525 punct ),blocked
R18991 T27539 T27525 prep in,blocked
R18992 T27540 T27541 compound TBST,buffer
R18993 T27541 T27539 pobj buffer,in
R18994 T27542 T27525 prep for,blocked
R18995 T27543 T27544 nummod 30,min
R18996 T27544 T27542 pobj min,for
R18997 T27545 T27544 prep at,min
R18998 T27546 T27547 compound room,temperature
R18999 T27547 T27545 pobj temperature,at
R19000 T27548 T27520 punct .,transferred
R19001 T27549 T27550 amod Primary,antibodies
R19002 T27550 T27552 nsubjpass antibodies,incubated
R19003 T27551 T27552 auxpass were,incubated
R19004 T27552 T27552 ROOT incubated,incubated
R19005 T27553 T27552 prep in,incubated
R19006 T27554 T27555 nummod 5,%
R19007 T27555 T27556 compound %,BSA/TBST
R19008 T27556 T27557 compound BSA/TBST,overnight
R19009 T27557 T27553 pobj overnight,in
R19010 T27558 T27552 prep at,incubated
R19011 T27559 T27560 nummod 4,°
R19012 T27560 T27563 nummod °,antibodies
R19013 T27561 T27563 nmod C.,antibodies
R19014 T27562 T27563 amod Secondary,antibodies
R19015 T27563 T27558 pobj antibodies,at
R19016 T27564 T27565 auxpass were,incubated
R19017 T27565 T27552 conj incubated,incubated
R19018 T27566 T27565 prep in,incubated
R19019 T27567 T27566 pobj TBST,in
R19020 T27568 T27565 prep for,incubated
R19021 T27569 T27570 nummod 30,min
R19022 T27570 T27568 pobj min,for
R19023 T27571 T27570 prep at,min
R19024 T27572 T27573 compound room,temperature
R19025 T27573 T27571 pobj temperature,at
R19026 T27574 T27552 punct .,incubated
R19027 T27575 T27575 ROOT Luminata,Luminata
R19028 T27576 T27577 punct (,Millipore
R19029 T27577 T27575 appos Millipore,Luminata
R19030 T27578 T27577 punct ),Millipore
R19031 T27579 T27580 compound ECL,reagents
R19032 T27580 T27582 nsubjpass reagents,used
R19033 T27581 T27582 auxpass were,used
R19034 T27582 T27582 ROOT used,used
R19035 T27583 T27584 aux to,develop
R19036 T27584 T27582 xcomp develop,used
R19037 T27585 T27586 det the,signals
R19038 T27586 T27584 dobj signals,develop
R19039 T27587 T27582 punct .,used
R19040 T27588 T27594 prep In,stripped
R19041 T27589 T27590 det some,cases
R19042 T27590 T27588 pobj cases,In
R19043 T27591 T27594 punct ",",stripped
R19044 T27592 T27594 nsubjpass membranes,stripped
R19045 T27593 T27594 auxpass were,stripped
R19046 T27594 T27594 ROOT stripped,stripped
R19047 T27595 T27594 advcl using,stripped
R19048 T27596 T27595 dobj OneMinute,using
R19049 T27597 T27595 conj stripping,using
R19050 T27598 T27597 dobj buffer,stripping
R19051 T27599 T27601 punct (,Biosciences
R19052 T27600 T27601 compound GM,Biosciences
R19053 T27601 T27598 appos Biosciences,buffer
R19054 T27602 T27601 punct ),Biosciences
R19055 T27603 T27597 cc and,stripping
R19056 T27604 T27597 conj reprobed,stripping
R19057 T27605 T27604 prep with,reprobed
R19058 T27606 T27607 amod new,antibodies
R19059 T27607 T27605 pobj antibodies,with
R19060 T27608 T27594 punct .,stripped
R18982 T27530 T27529 cc or,milk

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T27332 835-838 Protein denotes BSA
T27331 820-833 Protein denotes serum albumin
T27330 327-331 Protein denotes TNFα
T27329 297-308 Protein denotes µM zVAD.fmk
T27328 168-172 Protein denotes TNFα
T27327 138-149 Protein denotes µM zVAD.fmk

test3

Id Subject Object Predicate Lexical cue
T27058 327-331 Protein denotes TNFα
T27057 168-172 Protein denotes TNFα
T27056 327-331 Protein denotes TNFα
T27055 168-172 Protein denotes TNFα