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PMC:3585731 / 54466-55698 JSONTXT

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testone

Id Subject Object Predicate Lexical cue
T27054 340-344 Protein denotes TNFα
T27053 181-185 Protein denotes TNFα

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T27316 1221-1231 NN denotes antibodies
T27315 1217-1220 JJ denotes new
T27314 1212-1216 IN denotes with
T27313 1203-1211 VB denotes reprobed
T27312 1199-1202 CC denotes and
T27311 1197-1198 -RRB- denotes )
T27310 1186-1197 NNP denotes Biosciences
T27309 1183-1185 NNP denotes GM
T27308 1182-1183 -LRB- denotes (
T27307 1175-1181 NN denotes buffer
T27306 1165-1174 VB denotes stripping
T27305 1155-1164 NN denotes OneMinute
T27304 1149-1154 VB denotes using
T27303 1140-1148 VB denotes stripped
T27302 1135-1139 VB denotes were
T27301 1125-1134 NN denotes membranes
T27300 1123-1124 -COMMA- denotes ,
T27299 1118-1123 NN denotes cases
T27298 1113-1117 DT denotes some
T27297 1110-1112 IN denotes In
T27296 1101-1108 NN denotes signals
T27295 1097-1100 DT denotes the
T27294 1089-1096 VB denotes develop
T27293 1086-1088 TO denotes to
T27292 1081-1085 VB denotes used
T27291 1076-1080 VB denotes were
T27290 1067-1075 NN denotes reagents
T27289 1063-1066 NN denotes ECL
T27288 1061-1062 -RRB- denotes )
T27287 1052-1061 NNP denotes Millipore
T27286 1051-1052 -LRB- denotes (
T27285 1042-1050 NNP denotes Luminata
T27284 1029-1040 NN denotes temperature
T27283 1024-1028 NN denotes room
T27282 1021-1023 IN denotes at
T27281 1017-1020 NN denotes min
T27280 1014-1016 CD denotes 30
T27279 1010-1013 IN denotes for
T27278 1005-1009 NN denotes TBST
T27277 1002-1004 IN denotes in
T27276 992-1001 VB denotes incubated
T27275 987-991 VB denotes were
T27274 976-986 NN denotes antibodies
T27273 966-975 NNP denotes Secondary
T27272 961-965 NNP denotes 4°C.
T27271 958-960 IN denotes at
T27270 948-957 RB denotes overnight
T27269 939-947 NN denotes BSA/TBST
T27268 938-939 NN denotes %
T27267 937-938 CD denotes 5
T27266 934-936 IN denotes in
T27265 924-933 VB denotes incubated
T27264 919-923 VB denotes were
T27263 908-918 NN denotes antibodies
T27262 900-907 JJ denotes Primary
T27261 887-898 NN denotes temperature
T27260 882-886 NN denotes room
T27259 879-881 IN denotes at
T27258 875-878 NN denotes min
T27257 872-874 CD denotes 30
T27256 868-871 IN denotes for
T27255 861-867 NN denotes buffer
T27254 856-860 NN denotes TBST
T27253 853-855 IN denotes in
T27252 851-852 -RRB- denotes )
T27251 848-851 NN denotes BSA
T27250 847-848 -LRB- denotes (
T27249 839-846 NN denotes albumin
T27248 833-838 NN denotes serum
T27247 826-832 JJ denotes bovine
T27246 824-825 NN denotes %
T27245 823-824 CD denotes 5
T27244 820-822 CC denotes or
T27243 815-819 NN denotes milk
T27242 813-814 NN denotes %
T27241 812-813 CD denotes 3
T27240 809-811 IN denotes in
T27239 801-808 VB denotes blocked
T27238 799-800 -COMMA- denotes ,
T27237 791-799 NN denotes membrane
T27236 786-790 NN denotes PVDF
T27235 783-785 TO denotes to
T27234 771-782 VB denotes transferred
T27233 766-770 VB denotes were
T27232 761-765 NN denotes gels
T27231 752-760 NN denotes SDS-PAGE
T27230 750-751 -COMMA- denotes ,
T27229 743-750 RB denotes Briefly
T27228 732-741 NN denotes protocols
T27227 723-731 JJ denotes standard
T27226 720-722 TO denotes to
T27225 710-719 VB denotes according
T27224 700-709 VB denotes performed
T27223 696-699 VB denotes was
T27222 687-695 NN denotes blotting
T27221 679-686 NNP denotes Western
T27220 673-678 NNP denotes 95°C.
T27219 670-672 IN denotes at
T27218 666-669 NN denotes min
T27217 664-665 CD denotes 5
T27216 660-663 IN denotes for
T27215 653-659 VB denotes boiled
T27214 648-652 VB denotes were
T27213 639-647 NN denotes proteins
T27212 636-638 IN denotes of
T27211 628-635 NN denotes amounts
T27210 622-627 JJ denotes Equal
T27209 619-620 -RRB- denotes )
T27208 613-619 NNP denotes Pierce
T27207 612-613 -LRB- denotes (
T27206 604-611 NN denotes Reagent
T27205 598-603 NN denotes Assay
T27204 595-597 NN denotes nm
T27203 591-594 CD denotes 660
T27202 584-590 NNP denotes Pierce
T27201 580-583 DT denotes the
T27200 574-579 VB denotes using
T27199 565-573 VB denotes measured
T27198 560-564 VB denotes were
T27197 545-559 NN denotes concentrations
T27196 537-544 NN denotes Protein
T27195 525-535 NN denotes 14,000×rpm
T27194 522-524 IN denotes at
T27193 518-521 NN denotes min
T27192 515-517 CD denotes 15
T27191 511-514 IN denotes for
T27190 506-510 RP denotes down
T27189 501-505 VB denotes spun
T27188 496-500 VB denotes were
T27187 488-495 NN denotes lysates
T27186 483-487 NN denotes cell
T27185 481-482 -COMMA- denotes ,
T27184 471-481 NN denotes sonication
T27183 465-470 JJ denotes brief
T27182 459-464 IN denotes After
T27181 428-457 NN denotes phenylmethanesulfonylfluoride
T27180 422-427 NN denotes µg/ml
T27179 419-421 CD denotes 50
T27178 414-418 IN denotes with
T27177 401-413 VB denotes supplemented
T27176 399-400 -RRB- denotes )
T27175 390-399 NN denotes Signaling
T27174 385-389 NN denotes Cell
T27173 384-385 -LRB- denotes (
T27172 377-383 NN denotes buffer
T27171 370-376 NN denotes 1×RIPA
T27170 367-369 IN denotes in
T27169 357-366 VB denotes harvested
T27168 352-356 VB denotes were
T27167 346-351 NN denotes Cells
T27166 340-344 NN denotes TNFα
T27165 334-339 NN denotes mouse
T27164 328-333 NN denotes ng/ml
T27163 325-327 CD denotes 10
T27162 322-324 CC denotes or
T27161 313-321 NN denotes zVAD.fmk
T27160 310-312 NN denotes µM
T27159 307-309 CD denotes 20
T27158 305-306 -COMMA- denotes ,
T27157 298-305 NN denotes factors
T27156 291-297 NN denotes growth
T27155 288-290 IN denotes of
T27154 279-287 NN denotes addition
T27153 275-278 DT denotes the
T27152 272-274 TO denotes to
T27151 266-271 JJ denotes prior
T27150 263-265 NN denotes hr
T27149 260-262 CD denotes 24
T27148 256-259 IN denotes for
T27147 248-255 VB denotes starved
T27146 242-247 NN denotes serum
T27145 237-241 VB denotes were
T27144 231-236 NN denotes cells
T27143 229-230 -COMMA- denotes ,
T27142 219-229 NN denotes conditions
T27141 214-218 JJ denotes free
T27140 208-213 NN denotes serum
T27139 202-207 IN denotes under
T27138 191-201 NN denotes treatments
T27137 187-190 IN denotes For
T27136 181-185 NN denotes TNFα
T27135 175-180 NN denotes mouse
T27134 169-174 NN denotes ng/ml
T27133 166-168 CD denotes 10
T27132 163-165 CC denotes or
T27131 154-162 NN denotes zVAD.fmk
T27130 151-153 NN denotes µM
T27123 117-119 NN denotes hr
T27122 111-116 CD denotes 24–48
T27121 105-110 IN denotes After
T27120 97-103 NN denotes dishes
T27119 93-96 NN denotes mm2
T27118 90-92 CD denotes 35
T27117 85-89 IN denotes into
T27116 78-84 VB denotes seeded
T27115 73-77 VB denotes were
T27114 71-72 -RRB- denotes )
T27113 66-71 NN denotes cells
T27112 59-65 NN denotes Jurkat
T27111 53-58 CD denotes 1×106
T27110 52-53 -LRB- denotes (
T27109 46-51 NN denotes cells
T27108 37-45 JJ denotes adherent
T27107 31-36 CD denotes 4×105
T27106 29-30 -COMMA- denotes ,
T27105 25-29 NN denotes blot
T27104 17-24 JJ denotes Western
T27103 13-16 IN denotes For
T27102 8-12 NNP denotes Blot
T27101 0-7 NNP denotes Western
T27129 148-150 CD denotes 30
T27128 143-147 IN denotes with
T27127 132-142 VB denotes stimulated
T27126 127-131 VB denotes were
T27125 121-126 NN denotes cells
T27124 119-120 -COMMA- denotes ,
R18601 T27102 T27101 arg1Of Blot,Western
R18602 T27105 T27103 arg2Of blot,For
R18603 T27105 T27104 arg1Of blot,Western
R18604 T27109 T27107 arg1Of cells,4×105
R18605 T27109 T27108 arg1Of cells,adherent
R18606 T27109 T27110 arg1Of cells,(
R18607 T27109 T27115 arg1Of cells,were
R18608 T27109 T27116 arg2Of cells,seeded
R18609 T27113 T27110 arg2Of cells,(
R18610 T27113 T27111 arg1Of cells,1×106
R18611 T27113 T27112 arg1Of cells,Jurkat
R18612 T27114 T27110 arg3Of ),(
R18613 T27116 T27103 arg1Of seeded,For
R18614 T27116 T27106 arg1Of seeded,","
R18615 T27116 T27115 arg2Of seeded,were
R18616 T27116 T27117 arg1Of seeded,into
R18617 T27120 T27117 arg2Of dishes,into
R18618 T27120 T27118 arg1Of dishes,35
R18619 T27120 T27119 arg1Of dishes,mm2
R18620 T27123 T27121 arg2Of hr,After
R18621 T27123 T27122 arg1Of hr,24–48
R18622 T27125 T27126 arg1Of cells,were
R18623 T27125 T27127 arg2Of cells,stimulated
R18624 T27127 T27121 arg1Of stimulated,After
R18625 T27127 T27124 arg1Of stimulated,","
R18626 T27127 T27126 arg2Of stimulated,were
R18627 T27127 T27128 arg1Of stimulated,with
R18628 T27129 T27130 arg1Of 30,µM
R18629 T27131 T27129 arg1Of zVAD.fmk,30
R18630 T27131 T27132 arg1Of zVAD.fmk,or
R18631 T27132 T27128 arg2Of or,with
R18632 T27136 T27132 arg2Of TNFα,or
R18633 T27136 T27133 arg1Of TNFα,10
R18634 T27136 T27134 arg1Of TNFα,ng/ml
R18635 T27136 T27135 arg1Of TNFα,mouse
R18636 T27138 T27137 arg2Of treatments,For
R18637 T27138 T27139 arg1Of treatments,under
R18638 T27142 T27139 arg2Of conditions,under
R18639 T27142 T27140 arg1Of conditions,serum
R18640 T27142 T27141 arg1Of conditions,free
R18641 T27144 T27145 arg1Of cells,were
R18642 T27145 T27137 arg1Of were,For
R18643 T27145 T27143 arg1Of were,","
R18644 T27146 T27145 arg2Of serum,were
R18645 T27146 T27147 arg2Of serum,starved
R18646 T27147 T27148 arg1Of starved,for
R18647 T27150 T27148 arg2Of hr,for
R18648 T27150 T27149 arg1Of hr,24
R18649 T27150 T27151 arg1Of hr,prior
R18650 T27151 T27152 arg1Of prior,to
R18651 T27154 T27152 arg2Of addition,to
R18652 T27154 T27153 arg1Of addition,the
R18653 T27154 T27155 arg1Of addition,of
R18654 T27154 T27158 arg1Of addition,","
R18655 T27157 T27155 arg2Of factors,of
R18656 T27157 T27156 arg1Of factors,growth
R18657 T27159 T27160 arg1Of 20,µM
R18658 T27161 T27159 arg1Of zVAD.fmk,20
R18659 T27161 T27162 arg1Of zVAD.fmk,or
R18660 T27162 T27158 arg2Of or,","
R18661 T27166 T27162 arg2Of TNFα,or
R18662 T27166 T27163 arg1Of TNFα,10
R18663 T27166 T27164 arg1Of TNFα,ng/ml
R18664 T27166 T27165 arg1Of TNFα,mouse
R18665 T27167 T27168 arg1Of Cells,were
R18666 T27167 T27169 arg2Of Cells,harvested
R18667 T27169 T27168 arg2Of harvested,were
R18668 T27169 T27170 arg1Of harvested,in
R18669 T27172 T27170 arg2Of buffer,in
R18670 T27172 T27171 arg1Of buffer,1×RIPA
R18671 T27172 T27173 arg1Of buffer,(
R18672 T27172 T27177 arg2Of buffer,supplemented
R18673 T27175 T27173 arg2Of Signaling,(
R18674 T27175 T27174 arg1Of Signaling,Cell
R18675 T27176 T27173 arg3Of ),(
R18676 T27177 T27178 arg1Of supplemented,with
R18677 T27181 T27178 arg2Of phenylmethanesulfonylfluoride,with
R18678 T27181 T27179 arg1Of phenylmethanesulfonylfluoride,50
R18679 T27181 T27180 arg1Of phenylmethanesulfonylfluoride,µg/ml
R18680 T27184 T27182 arg2Of sonication,After
R18681 T27184 T27183 arg1Of sonication,brief
R18682 T27187 T27186 arg1Of lysates,cell
R18683 T27187 T27188 arg1Of lysates,were
R18684 T27187 T27189 arg2Of lysates,spun
R18685 T27189 T27182 arg1Of spun,After
R18686 T27189 T27185 arg1Of spun,","
R18687 T27189 T27188 arg2Of spun,were
R18688 T27189 T27190 arg1Of spun,down
R18689 T27189 T27191 arg1Of spun,for
R18690 T27189 T27194 arg1Of spun,at
R18691 T27193 T27191 arg2Of min,for
R18692 T27193 T27192 arg1Of min,15
R18693 T27195 T27194 arg2Of "14,000×rpm",at
R18694 T27197 T27196 arg1Of concentrations,Protein
R18695 T27197 T27198 arg1Of concentrations,were
R18696 T27197 T27199 arg2Of concentrations,measured
R18697 T27199 T27198 arg2Of measured,were
R18698 T27200 T27199 arg3Of using,measured
R18699 T27200 T27207 arg1Of using,(
R18700 T27202 T27203 arg1Of Pierce,660
R18701 T27206 T27200 arg2Of Reagent,using
R18702 T27206 T27201 arg1Of Reagent,the
R18703 T27206 T27202 arg1Of Reagent,Pierce
R18704 T27206 T27204 arg1Of Reagent,nm
R18705 T27206 T27205 arg1Of Reagent,Assay
R18706 T27208 T27207 arg2Of Pierce,(
R18707 T27209 T27207 arg3Of ),(
R18708 T27211 T27210 arg1Of amounts,Equal
R18709 T27211 T27212 arg1Of amounts,of
R18710 T27211 T27214 arg1Of amounts,were
R18711 T27211 T27215 arg2Of amounts,boiled
R18712 T27213 T27212 arg2Of proteins,of
R18713 T27215 T27214 arg2Of boiled,were
R18714 T27215 T27216 arg1Of boiled,for
R18715 T27218 T27216 arg2Of min,for
R18716 T27218 T27217 arg1Of min,5
R18717 T27218 T27219 arg1Of min,at
R18718 T27221 T27219 arg2Of Western,at
R18719 T27221 T27220 arg1Of Western,95°C.
R18720 T27222 T27223 arg1Of blotting,was
R18721 T27222 T27224 arg2Of blotting,performed
R18722 T27224 T27214 modOf performed,were
R18723 T27224 T27223 arg2Of performed,was
R18724 T27224 T27225 arg1Of performed,according
R18725 T27226 T27225 arg2Of to,according
R18726 T27228 T27226 arg2Of protocols,to
R18727 T27228 T27227 arg1Of protocols,standard
R18728 T27232 T27231 arg1Of gels,SDS-PAGE
R18729 T27232 T27233 arg1Of gels,were
R18730 T27232 T27234 arg2Of gels,transferred
R18731 T27232 T27239 arg1Of gels,blocked
R18732 T27234 T27233 arg2Of transferred,were
R18733 T27234 T27235 arg1Of transferred,to
R18734 T27234 T27238 arg1Of transferred,","
R18735 T27237 T27235 arg2Of membrane,to
R18736 T27237 T27236 arg1Of membrane,PVDF
R18737 T27238 T27229 arg1Of ",",Briefly
R18738 T27238 T27230 arg1Of ",",","
R18739 T27238 T27256 arg1Of ",",for
R18740 T27239 T27238 arg2Of blocked,","
R18741 T27239 T27240 arg1Of blocked,in
R18742 T27242 T27240 arg2Of %,in
R18743 T27242 T27241 arg1Of %,3
R18744 T27243 T27244 arg1Of milk,or
R18745 T27244 T27239 arg2Of or,blocked
R18746 T27245 T27246 arg1Of 5,%
R18747 T27249 T27244 arg2Of albumin,or
R18748 T27249 T27245 arg1Of albumin,5
R18749 T27249 T27247 arg1Of albumin,bovine
R18750 T27249 T27248 arg1Of albumin,serum
R18751 T27249 T27250 arg1Of albumin,(
R18752 T27249 T27253 arg1Of albumin,in
R18753 T27251 T27250 arg2Of BSA,(
R18754 T27252 T27250 arg3Of ),(
R18755 T27255 T27253 arg2Of buffer,in
R18756 T27255 T27254 arg1Of buffer,TBST
R18757 T27258 T27256 arg2Of min,for
R18758 T27258 T27257 arg1Of min,30
R18759 T27258 T27259 arg1Of min,at
R18760 T27261 T27259 arg2Of temperature,at
R18761 T27261 T27260 arg1Of temperature,room
R18762 T27263 T27262 arg1Of antibodies,Primary
R18763 T27263 T27264 arg1Of antibodies,were
R18764 T27263 T27265 arg2Of antibodies,incubated
R18765 T27265 T27264 arg2Of incubated,were
R18766 T27265 T27266 arg1Of incubated,in
R18767 T27265 T27270 arg1Of incubated,overnight
R18768 T27267 T27268 arg1Of 5,%
R18769 T27269 T27266 arg2Of BSA/TBST,in
R18770 T27269 T27267 arg1Of BSA/TBST,5
R18771 T27270 T27275 modOf overnight,were
R18772 T27273 T27272 arg1Of Secondary,4°C.
R18773 T27274 T27273 arg1Of antibodies,Secondary
R18774 T27274 T27275 arg1Of antibodies,were
R18775 T27274 T27276 arg2Of antibodies,incubated
R18776 T27276 T27271 arg1Of incubated,at
R18777 T27276 T27275 arg2Of incubated,were
R18778 T27276 T27277 arg1Of incubated,in
R18779 T27276 T27279 arg1Of incubated,for
R18780 T27276 T27282 arg1Of incubated,at
R18781 T27278 T27277 arg2Of TBST,in
R18782 T27281 T27279 arg2Of min,for
R18783 T27281 T27280 arg1Of min,30
R18784 T27284 T27282 arg2Of temperature,at
R18785 T27284 T27283 arg1Of temperature,room
R18786 T27287 T27286 arg2Of Millipore,(
R18787 T27288 T27286 arg3Of ),(
R18788 T27290 T27285 arg1Of reagents,Luminata
R18789 T27290 T27286 arg1Of reagents,(
R18790 T27290 T27289 arg1Of reagents,ECL
R18791 T27290 T27291 arg1Of reagents,were
R18792 T27290 T27292 arg2Of reagents,used
R18793 T27292 T27291 arg2Of used,were
R18794 T27294 T27292 arg3Of develop,used
R18795 T27294 T27293 arg1Of develop,to
R18796 T27296 T27294 arg2Of signals,develop
R18797 T27296 T27295 arg1Of signals,the
R18798 T27299 T27297 arg2Of cases,In
R18799 T27299 T27298 arg1Of cases,some
R18800 T27301 T27302 arg1Of membranes,were
R18801 T27301 T27303 arg2Of membranes,stripped
R18802 T27301 T27313 arg2Of membranes,reprobed
R18803 T27303 T27312 arg1Of stripped,and
R18804 T27304 T27303 arg3Of using,stripped
R18805 T27305 T27304 arg2Of OneMinute,using
R18806 T27305 T27306 arg1Of OneMinute,stripping
R18807 T27307 T27306 arg2Of buffer,stripping
R18808 T27307 T27308 arg1Of buffer,(
R18809 T27310 T27308 arg2Of Biosciences,(
R18810 T27310 T27309 arg1Of Biosciences,GM
R18811 T27311 T27308 arg3Of ),(
R18812 T27312 T27297 arg1Of and,In
R18813 T27312 T27300 arg1Of and,","
R18814 T27312 T27302 arg2Of and,were
R18815 T27313 T27312 arg2Of reprobed,and
R18816 T27313 T27314 arg1Of reprobed,with
R18817 T27316 T27314 arg2Of antibodies,with
R18818 T27316 T27315 arg1Of antibodies,new

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T27078 340-344 Protein denotes TNFα
T27077 181-185 Protein denotes TNFα

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T27320 340-344 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T27319 181-185 http://www.uniprot.org/uniprot/P01375 denotes TNFα

UBERON-AE

Id Subject Object Predicate Lexical cue
T27062 815-819 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T27061 833-838 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T27060 242-247 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T27059 208-213 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T27080 1101-1108 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T27079 291-297 http://purl.obolibrary.org/obo/GO_0040007 denotes growth

GO-MF

Id Subject Object Predicate Lexical cue
T27083 1221-1231 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27082 976-986 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27081 908-918 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T27096 1221-1231 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27095 976-986 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27094 908-918 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27093 1221-1231 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27092 976-986 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27091 908-918 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27090 1125-1134 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T27089 791-799 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T27088 483-487 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T27087 231-236 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27086 121-126 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27085 66-71 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27084 46-51 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T27074 1110-1232 Sentence denotes In some cases, membranes were stripped using OneMinute stripping buffer (GM Biosciences) and reprobed with new antibodies.
T27073 1042-1109 Sentence denotes Luminata (Millipore) ECL reagents were used to develop the signals.
T27072 900-1041 Sentence denotes Primary antibodies were incubated in 5%BSA/TBST overnight at 4°C. Secondary antibodies were incubated in TBST for 30 min at room temperature.
T27071 743-899 Sentence denotes Briefly, SDS-PAGE gels were transferred to PVDF membrane, blocked in 3% milk or 5% bovine serum albumin (BSA) in TBST buffer for 30 min at room temperature.
T27070 622-742 Sentence denotes Equal amounts of proteins were boiled for 5 min at 95°C. Western blotting was performed according to standard protocols.
T27069 537-621 Sentence denotes Protein concentrations were measured using the Pierce 660 nm Assay Reagent (Pierce).
T27068 459-536 Sentence denotes After brief sonication, cell lysates were spun down for 15 min at 14,000×rpm.
T27067 346-458 Sentence denotes Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.
T27066 187-345 Sentence denotes For treatments under serum free conditions, cells were serum starved for 24 hr prior to the addition of growth factors, 20 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T27065 105-186 Sentence denotes After 24–48 hr, cells were stimulated with 30 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T27064 13-104 Sentence denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes.
T27063 0-12 Sentence denotes Western Blot
T341 0-12 Sentence denotes Western Blot
T342 13-104 Sentence denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes.
T343 105-186 Sentence denotes After 24–48 hr, cells were stimulated with 30 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T344 187-345 Sentence denotes For treatments under serum free conditions, cells were serum starved for 24 hr prior to the addition of growth factors, 20 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T345 346-458 Sentence denotes Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.
T346 459-536 Sentence denotes After brief sonication, cell lysates were spun down for 15 min at 14,000×rpm.
T347 537-621 Sentence denotes Protein concentrations were measured using the Pierce 660 nm Assay Reagent (Pierce).
T348 622-678 Sentence denotes Equal amounts of proteins were boiled for 5 min at 95°C.
T349 679-742 Sentence denotes Western blotting was performed according to standard protocols.
T350 743-899 Sentence denotes Briefly, SDS-PAGE gels were transferred to PVDF membrane, blocked in 3% milk or 5% bovine serum albumin (BSA) in TBST buffer for 30 min at room temperature.
T351 900-965 Sentence denotes Primary antibodies were incubated in 5%BSA/TBST overnight at 4°C.
T352 966-1041 Sentence denotes Secondary antibodies were incubated in TBST for 30 min at room temperature.
T353 1042-1109 Sentence denotes Luminata (Millipore) ECL reagents were used to develop the signals.
T354 1110-1232 Sentence denotes In some cases, membranes were stripped using OneMinute stripping buffer (GM Biosciences) and reprobed with new antibodies.

simple1

Id Subject Object Predicate Lexical cue
T27100 340-344 Protein denotes TNFα
T27099 181-185 Protein denotes TNFα

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T27318 340-344 Protein denotes TNFα
T27317 181-185 Protein denotes TNFα

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T27326 340-344 Protein denotes TNFα
T27325 181-185 Protein denotes TNFα

DLUT931

Id Subject Object Predicate Lexical cue
T27322 340-344 Protein denotes TNFα
T27321 181-185 Protein denotes TNFα

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T27345 53-71 Entity denotes 1×106 Jurkat cells
T27344 537-559 Entity denotes Protein concentrations
T27343 900-918 Entity denotes Primary antibodies
T27342 148-162 Entity denotes 30 µM zVAD.fmk
T27341 786-790 Entity denotes PVDF
T27340 823-852 Protein denotes 5% bovine serum albumin (BSA)
T27339 636-647 Protein denotes of proteins
T27366 346-376 Binding denotes Cells were harvested in 1×RIPA
T27365 13-103 Binding denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes
T27364 595-620 Entity denotes nm Assay Reagent (Pierce)
T27363 231-236 Entity denotes cells
T27362 1063-1075 Entity denotes ECL reagents
T27361 786-799 Entity denotes PVDF membrane
T27360 966-986 Entity denotes Secondary antibodies
T27359 31-72 Entity denotes 4×105 adherent cells (1×106 Jurkat cells)
T27358 121-126 Entity denotes cells
T27357 1183-1185 Entity denotes GM
T27356 346-351 Entity denotes Cells
T27355 1217-1231 Entity denotes new antibodies
T27354 385-389 Entity denotes Cell
T27353 313-321 Entity denotes zVAD.fmk
T27352 483-495 Entity denotes cell lysates
T27351 1063-1066 Protein denotes ECL
T27350 1125-1134 Entity denotes membranes
T27349 166-185 Protein denotes 10 ng/ml mouse TNFα
T27348 307-344 Protein denotes 20 µM zVAD.fmk or 10 ng/ml mouse TNFα
T27347 752-765 Entity denotes SDS-PAGE gels
T27346 848-851 Protein denotes BSA

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T27575 1042-1050 NNP denotes Luminata
T27608 1231-1232 . denotes .
T27607 1221-1231 NNS denotes antibodies
T27606 1217-1220 JJ denotes new
T27605 1212-1216 IN denotes with
T27604 1203-1211 VBN denotes reprobed
T27603 1199-1202 CC denotes and
T27602 1197-1198 -RRB- denotes )
T27601 1186-1197 NNPS denotes Biosciences
T27600 1183-1185 NNP denotes GM
T27599 1182-1183 -LRB- denotes (
T27598 1175-1181 NN denotes buffer
T27597 1165-1174 VBG denotes stripping
T27596 1155-1164 NNP denotes OneMinute
T27595 1149-1154 VBG denotes using
T27594 1140-1148 VBN denotes stripped
T27593 1135-1139 VBD denotes were
T27592 1125-1134 NNS denotes membranes
T27591 1123-1124 , denotes ,
T27590 1118-1123 NNS denotes cases
T27589 1113-1117 DT denotes some
T27588 1110-1112 IN denotes In
T27587 1108-1109 . denotes .
T27586 1101-1108 NNS denotes signals
T27585 1097-1100 DT denotes the
T27584 1089-1096 VB denotes develop
T27583 1086-1088 TO denotes to
T27582 1081-1085 VBN denotes used
T27581 1076-1080 VBD denotes were
T27580 1067-1075 NNS denotes reagents
T27579 1063-1066 NNP denotes ECL
T27578 1061-1062 -RRB- denotes )
T27577 1052-1061 NNP denotes Millipore
T27576 1051-1052 -LRB- denotes (
T27574 1040-1041 . denotes .
T27573 1029-1040 NN denotes temperature
T27572 1024-1028 NN denotes room
T27571 1021-1023 IN denotes at
T27570 1017-1020 NN denotes min
T27569 1014-1016 CD denotes 30
T27568 1010-1013 IN denotes for
T27567 1005-1009 NNP denotes TBST
T27566 1002-1004 IN denotes in
T27565 992-1001 VBN denotes incubated
T27564 987-991 VBD denotes were
T27563 976-986 NNS denotes antibodies
T27562 966-975 JJ denotes Secondary
T27561 963-965 NNP denotes C.
T27560 962-963 CD denotes °
T27559 961-962 CD denotes 4
T27558 958-960 IN denotes at
T27557 948-957 JJ denotes overnight
T27556 939-947 NNP denotes BSA/TBST
T27555 938-939 NN denotes %
T27554 937-938 CD denotes 5
T27553 934-936 IN denotes in
T27552 924-933 VBN denotes incubated
T27551 919-923 VBD denotes were
T27550 908-918 NNS denotes antibodies
T27549 900-907 JJ denotes Primary
T27548 898-899 . denotes .
T27547 887-898 NN denotes temperature
T27546 882-886 NN denotes room
T27545 879-881 IN denotes at
T27544 875-878 NN denotes min
T27543 872-874 CD denotes 30
T27542 868-871 IN denotes for
T27541 861-867 NN denotes buffer
T27540 856-860 NNP denotes TBST
T27539 853-855 IN denotes in
T27538 851-852 -RRB- denotes )
T27537 848-851 NNP denotes BSA
T27536 847-848 -LRB- denotes (
T27535 839-846 NN denotes albumin
T27534 833-838 NN denotes serum
T27533 826-832 JJ denotes bovine
T27532 824-825 NN denotes %
T27531 823-824 CD denotes 5
T27530 820-822 CC denotes or
T27529 815-819 NN denotes milk
T27528 813-814 NN denotes %
T27527 812-813 CD denotes 3
T27526 809-811 IN denotes in
T27525 801-808 VBN denotes blocked
T27524 799-800 , denotes ,
T27523 791-799 NN denotes membrane
T27522 786-790 NNP denotes PVDF
T27521 783-785 TO denotes to
T27520 771-782 VBN denotes transferred
T27519 766-770 VBD denotes were
T27518 761-765 NNS denotes gels
T27517 752-760 NNP denotes SDS-PAGE
T27516 750-751 , denotes ,
T27515 743-750 RB denotes Briefly
T27514 741-742 . denotes .
T27513 732-741 NNS denotes protocols
T27512 723-731 JJ denotes standard
T27511 720-722 TO denotes to
T27510 710-719 VBG denotes according
T27509 700-709 VBN denotes performed
T27508 696-699 VBD denotes was
T27507 687-695 VBG denotes blotting
T27506 679-686 NNP denotes Western
T27505 676-678 NNP denotes C.
T27504 675-676 CD denotes °
T27503 673-675 CD denotes 95
T27502 670-672 IN denotes at
T27501 666-669 NN denotes min
T27500 664-665 CD denotes 5
T27499 660-663 IN denotes for
T27498 653-659 VBN denotes boiled
T27497 648-652 VBD denotes were
T27496 639-647 NNS denotes proteins
T27495 636-638 IN denotes of
T27494 628-635 NNS denotes amounts
T27493 622-627 NNP denotes Equal
T27492 620-621 . denotes .
T27491 619-620 -RRB- denotes )
T27490 613-619 NNP denotes Pierce
T27489 612-613 -LRB- denotes (
T27488 604-611 NNP denotes Reagent
T27487 598-603 NNP denotes Assay
T27486 595-597 NN denotes nm
T27485 591-594 CD denotes 660
T27484 584-590 NNP denotes Pierce
T27483 580-583 DT denotes the
T27482 574-579 VBG denotes using
T27481 565-573 VBN denotes measured
T27480 560-564 VBD denotes were
T27479 545-559 NNS denotes concentrations
T27478 537-544 NN denotes Protein
T27477 535-536 . denotes .
T27476 532-535 NN denotes rpm
T27475 531-532 CD denotes ×
T27474 525-531 CD denotes 14,000
T27473 522-524 IN denotes at
T27472 518-521 NN denotes min
T27471 515-517 CD denotes 15
T27470 511-514 IN denotes for
T27469 506-510 RP denotes down
T27468 501-505 VBN denotes spun
T27467 496-500 VBD denotes were
T27466 488-495 NNS denotes lysates
T27465 483-487 NN denotes cell
T27464 481-482 , denotes ,
T27463 471-481 NN denotes sonication
T27462 465-470 JJ denotes brief
T27461 459-464 IN denotes After
T27460 457-458 . denotes .
T27459 428-457 NN denotes phenylmethanesulfonylfluoride
T27458 425-427 NN denotes ml
T27457 424-425 NN denotes /
T27456 422-424 NN denotes µg
T27455 419-421 CD denotes 50
T27454 414-418 IN denotes with
T27453 401-413 VBN denotes supplemented
T27452 399-400 -RRB- denotes )
T27451 390-399 NNP denotes Signaling
T27450 385-389 NNP denotes Cell
T27449 384-385 -LRB- denotes (
T27448 377-383 NN denotes buffer
T27447 372-376 NNP denotes RIPA
T27446 371-372 CD denotes ×
T27445 370-371 CD denotes 1
T27444 367-369 IN denotes in
T27443 357-366 VBN denotes harvested
T27442 352-356 VBD denotes were
T27441 346-351 NNS denotes Cells
T27440 344-345 . denotes .
T27439 340-344 NNP denotes TNFα
T27438 334-339 NN denotes mouse
T27437 328-333 JJ denotes ng/ml
T27436 325-327 CD denotes 10
T27435 322-324 CC denotes or
T27434 313-321 NNP denotes zVAD.fmk
T27433 310-312 NNP denotes µM
T27432 307-309 CD denotes 20
T27431 305-306 , denotes ,
T27430 298-305 NNS denotes factors
T27429 291-297 NN denotes growth
T27428 288-290 IN denotes of
T27427 279-287 NN denotes addition
T27426 275-278 DT denotes the
T27425 272-274 TO denotes to
T27424 266-271 RB denotes prior
T27423 263-265 NN denotes hr
T27422 260-262 CD denotes 24
T27421 256-259 IN denotes for
T27420 248-255 VBN denotes starved
T27419 242-247 JJ denotes serum
T27418 237-241 VBD denotes were
T27417 231-236 NNS denotes cells
T27416 229-230 , denotes ,
T27415 219-229 NNS denotes conditions
T27414 214-218 JJ denotes free
T27413 208-213 NN denotes serum
T27412 202-207 IN denotes under
T27411 191-201 NNS denotes treatments
T27410 187-190 IN denotes For
T27409 185-186 . denotes .
T27408 181-185 NNP denotes TNFα
T27407 175-180 NN denotes mouse
T27406 169-174 JJ denotes ng/ml
T27405 166-168 CD denotes 10
T27404 163-165 CC denotes or
T27403 154-162 NNP denotes zVAD.fmk
T27402 151-153 NNP denotes µM
T27401 148-150 CD denotes 30
T27400 143-147 IN denotes with
T27399 132-142 VBN denotes stimulated
T27398 127-131 VBD denotes were
T27397 121-126 NNS denotes cells
T27396 119-120 , denotes ,
T27395 117-119 NN denotes hr
T27394 114-116 CD denotes 48
T27393 111-113 CD denotes 24
T27392 105-110 IN denotes After
T27391 103-104 . denotes .
T27390 97-103 NNS denotes dishes
T27389 93-96 CD denotes mm2
T27388 90-92 CD denotes 35
T27387 85-89 IN denotes into
T27386 78-84 VBN denotes seeded
T27385 73-77 VBD denotes were
T27384 71-72 -RRB- denotes )
T27383 66-71 NNS denotes cells
T27382 59-65 NN denotes Jurkat
T27381 55-58 CD denotes 106
T27380 54-55 CD denotes ×
T27379 53-54 CD denotes 1
T27378 52-53 -LRB- denotes (
T27377 46-51 NNS denotes cells
T27376 37-45 JJ denotes adherent
T27375 33-36 CD denotes 105
T27374 32-33 CD denotes ×
T27373 31-32 CD denotes 4
T27372 29-30 , denotes ,
T27371 25-29 NN denotes blot
T27370 17-24 JJ denotes Western
T27369 13-16 IN denotes For
T27368 8-12 NN denotes Blot
T27367 0-7 JJ denotes Western
R18819 T27367 T27368 amod Western,Blot
R18820 T27368 T27386 nsubjpass Blot,seeded
R18821 T27369 T27386 prep For,seeded
R18822 T27370 T27371 amod Western,blot
R18823 T27371 T27369 pobj blot,For
R18824 T27372 T27386 punct ",",seeded
R18825 T27373 T27377 nummod 4,cells
R18826 T27374 T27377 nummod ×,cells
R18827 T27375 T27377 nummod 105,cells
R18828 T27376 T27377 amod adherent,cells
R18829 T27377 T27386 nsubjpass cells,seeded
R18830 T27378 T27377 punct (,cells
R18831 T27379 T27383 nummod 1,cells
R18832 T27380 T27383 nummod ×,cells
R18833 T27381 T27383 nummod 106,cells
R18834 T27382 T27383 compound Jurkat,cells
R18835 T27383 T27377 appos cells,cells
R18836 T27384 T27377 punct ),cells
R18837 T27385 T27386 auxpass were,seeded
R18838 T27386 T27386 ROOT seeded,seeded
R18839 T27387 T27386 prep into,seeded
R18840 T27388 T27390 nummod 35,dishes
R18841 T27389 T27390 nummod mm2,dishes
R18842 T27390 T27387 pobj dishes,into
R18843 T27391 T27386 punct .,seeded
R18844 T27392 T27399 prep After,stimulated
R18845 T27393 T27392 pobj 24,After
R18846 T27394 T27395 nummod 48,hr
R18847 T27395 T27393 appos hr,24
R18848 T27396 T27399 punct ",",stimulated
R18849 T27397 T27399 nsubjpass cells,stimulated
R18850 T27398 T27399 auxpass were,stimulated
R18851 T27399 T27399 ROOT stimulated,stimulated
R18852 T27400 T27399 prep with,stimulated
R18853 T27401 T27403 nummod 30,zVAD.fmk
R18854 T27402 T27403 compound µM,zVAD.fmk
R18855 T27403 T27400 pobj zVAD.fmk,with
R18856 T27404 T27403 cc or,zVAD.fmk
R18857 T27405 T27407 nummod 10,mouse
R18858 T27406 T27407 amod ng/ml,mouse
R18859 T27407 T27408 compound mouse,TNFα
R18860 T27408 T27403 conj TNFα,zVAD.fmk
R18861 T27409 T27399 punct .,stimulated
R18862 T27410 T27420 prep For,starved
R18863 T27411 T27410 pobj treatments,For
R18864 T27412 T27411 prep under,treatments
R18865 T27413 T27415 nmod serum,conditions
R18866 T27414 T27415 amod free,conditions
R18867 T27415 T27412 pobj conditions,under
R18868 T27416 T27420 punct ",",starved
R18869 T27417 T27420 nsubjpass cells,starved
R18870 T27418 T27420 auxpass were,starved
R18871 T27419 T27420 advmod serum,starved
R18872 T27420 T27420 ROOT starved,starved
R18873 T27421 T27420 prep for,starved
R18874 T27422 T27423 nummod 24,hr
R18875 T27423 T27421 pobj hr,for
R18876 T27424 T27420 advmod prior,starved
R18877 T27425 T27424 prep to,prior
R18878 T27426 T27427 det the,addition
R18879 T27427 T27425 pobj addition,to
R18880 T27428 T27427 prep of,addition
R18881 T27429 T27430 compound growth,factors
R18882 T27430 T27428 pobj factors,of
R18883 T27431 T27430 punct ",",factors
R18884 T27432 T27434 nummod 20,zVAD.fmk
R18885 T27433 T27434 compound µM,zVAD.fmk
R18886 T27434 T27430 appos zVAD.fmk,factors
R18887 T27435 T27434 cc or,zVAD.fmk
R18888 T27436 T27439 nummod 10,TNFα
R18889 T27437 T27438 amod ng/ml,mouse
R18890 T27438 T27439 compound mouse,TNFα
R18891 T27439 T27434 conj TNFα,zVAD.fmk
R18892 T27440 T27420 punct .,starved
R18893 T27441 T27443 nsubjpass Cells,harvested
R18894 T27442 T27443 auxpass were,harvested
R18895 T27443 T27443 ROOT harvested,harvested
R18896 T27444 T27443 prep in,harvested
R18897 T27445 T27446 nummod 1,×
R18898 T27446 T27448 nummod ×,buffer
R18899 T27447 T27448 compound RIPA,buffer
R18900 T27448 T27444 pobj buffer,in
R18901 T27449 T27448 punct (,buffer
R18902 T27450 T27451 compound Cell,Signaling
R18903 T27451 T27448 appos Signaling,buffer
R18904 T27452 T27448 punct ),buffer
R18905 T27453 T27443 conj supplemented,harvested
R18906 T27454 T27453 prep with,supplemented
R18907 T27455 T27456 nummod 50,µg
R18908 T27456 T27459 nmod µg,phenylmethanesulfonylfluoride
R18909 T27457 T27459 nmod /,phenylmethanesulfonylfluoride
R18910 T27458 T27459 compound ml,phenylmethanesulfonylfluoride
R18911 T27459 T27454 pobj phenylmethanesulfonylfluoride,with
R18912 T27460 T27443 punct .,harvested
R18913 T27461 T27468 prep After,spun
R18914 T27462 T27463 amod brief,sonication
R18915 T27463 T27468 advcl sonication,spun
R18916 T27464 T27468 punct ",",spun
R18917 T27465 T27466 compound cell,lysates
R18918 T27466 T27468 nsubjpass lysates,spun
R18919 T27467 T27468 auxpass were,spun
R18920 T27468 T27468 ROOT spun,spun
R18921 T27469 T27468 prt down,spun
R18922 T27470 T27468 prep for,spun
R18923 T27471 T27472 nummod 15,min
R18924 T27472 T27470 pobj min,for
R18925 T27473 T27472 prep at,min
R18926 T27474 T27475 nummod "14,000",×
R18927 T27475 T27476 nummod ×,rpm
R18928 T27476 T27473 pobj rpm,at
R18929 T27477 T27468 punct .,spun
R18930 T27478 T27479 compound Protein,concentrations
R18931 T27479 T27481 nsubjpass concentrations,measured
R18932 T27480 T27481 auxpass were,measured
R18933 T27481 T27481 ROOT measured,measured
R18934 T27482 T27481 advcl using,measured
R18935 T27483 T27484 det the,Pierce
R18936 T27484 T27482 dobj Pierce,using
R18937 T27485 T27484 nummod 660,Pierce
R18938 T27486 T27488 compound nm,Reagent
R18939 T27487 T27488 compound Assay,Reagent
R18940 T27488 T27484 conj Reagent,Pierce
R18941 T27489 T27490 punct (,Pierce
R18942 T27490 T27488 appos Pierce,Reagent
R18943 T27491 T27488 punct ),Reagent
R18944 T27492 T27481 punct .,measured
R18945 T27493 T27494 compound Equal,amounts
R18946 T27494 T27498 nsubjpass amounts,boiled
R18947 T27495 T27494 prep of,amounts
R18948 T27496 T27495 pobj proteins,of
R18949 T27497 T27498 auxpass were,boiled
R18950 T27498 T27498 ROOT boiled,boiled
R18951 T27499 T27498 prep for,boiled
R18952 T27500 T27501 nummod 5,min
R18953 T27501 T27499 pobj min,for
R18954 T27502 T27498 prep at,boiled
R18955 T27503 T27504 nummod 95,°
R18956 T27504 T27507 nummod °,blotting
R18957 T27505 T27506 compound C.,Western
R18958 T27506 T27507 compound Western,blotting
R18959 T27507 T27502 pobj blotting,at
R18960 T27508 T27509 auxpass was,performed
R18961 T27509 T27498 conj performed,boiled
R18962 T27510 T27509 prep according,performed
R18963 T27511 T27510 prep to,according
R18964 T27512 T27513 amod standard,protocols
R18965 T27513 T27511 pobj protocols,to
R18966 T27514 T27498 punct .,boiled
R18967 T27515 T27520 advmod Briefly,transferred
R18968 T27516 T27520 punct ",",transferred
R18969 T27517 T27518 compound SDS-PAGE,gels
R18970 T27518 T27520 nsubjpass gels,transferred
R18971 T27519 T27520 auxpass were,transferred
R18972 T27520 T27520 ROOT transferred,transferred
R18973 T27521 T27520 prep to,transferred
R18974 T27522 T27523 compound PVDF,membrane
R18975 T27523 T27521 pobj membrane,to
R18976 T27524 T27523 punct ",",membrane
R18977 T27525 T27520 conj blocked,transferred
R18978 T27526 T27525 prep in,blocked
R18979 T27527 T27528 nummod 3,%
R18980 T27528 T27529 compound %,milk
R18981 T27529 T27526 pobj milk,in
R18983 T27531 T27532 nummod 5,%
R18984 T27532 T27535 compound %,albumin
R18985 T27533 T27534 amod bovine,serum
R18986 T27534 T27535 compound serum,albumin
R18987 T27535 T27529 conj albumin,milk
R18988 T27536 T27537 punct (,BSA
R18989 T27537 T27535 appos BSA,albumin
R18990 T27538 T27525 punct ),blocked
R18991 T27539 T27525 prep in,blocked
R18992 T27540 T27541 compound TBST,buffer
R18993 T27541 T27539 pobj buffer,in
R18994 T27542 T27525 prep for,blocked
R18995 T27543 T27544 nummod 30,min
R18996 T27544 T27542 pobj min,for
R18997 T27545 T27544 prep at,min
R18998 T27546 T27547 compound room,temperature
R18999 T27547 T27545 pobj temperature,at
R19000 T27548 T27520 punct .,transferred
R19001 T27549 T27550 amod Primary,antibodies
R19002 T27550 T27552 nsubjpass antibodies,incubated
R19003 T27551 T27552 auxpass were,incubated
R19004 T27552 T27552 ROOT incubated,incubated
R19005 T27553 T27552 prep in,incubated
R19006 T27554 T27555 nummod 5,%
R19007 T27555 T27556 compound %,BSA/TBST
R19008 T27556 T27557 compound BSA/TBST,overnight
R19009 T27557 T27553 pobj overnight,in
R19010 T27558 T27552 prep at,incubated
R19011 T27559 T27560 nummod 4,°
R19012 T27560 T27563 nummod °,antibodies
R19013 T27561 T27563 nmod C.,antibodies
R19014 T27562 T27563 amod Secondary,antibodies
R19015 T27563 T27558 pobj antibodies,at
R19016 T27564 T27565 auxpass were,incubated
R19017 T27565 T27552 conj incubated,incubated
R19018 T27566 T27565 prep in,incubated
R19019 T27567 T27566 pobj TBST,in
R19020 T27568 T27565 prep for,incubated
R19021 T27569 T27570 nummod 30,min
R19022 T27570 T27568 pobj min,for
R19023 T27571 T27570 prep at,min
R19024 T27572 T27573 compound room,temperature
R19025 T27573 T27571 pobj temperature,at
R19026 T27574 T27552 punct .,incubated
R19027 T27575 T27575 ROOT Luminata,Luminata
R19028 T27576 T27577 punct (,Millipore
R19029 T27577 T27575 appos Millipore,Luminata
R19030 T27578 T27577 punct ),Millipore
R19031 T27579 T27580 compound ECL,reagents
R19032 T27580 T27582 nsubjpass reagents,used
R19033 T27581 T27582 auxpass were,used
R19034 T27582 T27582 ROOT used,used
R19035 T27583 T27584 aux to,develop
R19036 T27584 T27582 xcomp develop,used
R19037 T27585 T27586 det the,signals
R19038 T27586 T27584 dobj signals,develop
R19039 T27587 T27582 punct .,used
R19040 T27588 T27594 prep In,stripped
R19041 T27589 T27590 det some,cases
R19042 T27590 T27588 pobj cases,In
R19043 T27591 T27594 punct ",",stripped
R19044 T27592 T27594 nsubjpass membranes,stripped
R19045 T27593 T27594 auxpass were,stripped
R19046 T27594 T27594 ROOT stripped,stripped
R19047 T27595 T27594 advcl using,stripped
R19048 T27596 T27595 dobj OneMinute,using
R19049 T27597 T27595 conj stripping,using
R19050 T27598 T27597 dobj buffer,stripping
R19051 T27599 T27601 punct (,Biosciences
R19052 T27600 T27601 compound GM,Biosciences
R19053 T27601 T27598 appos Biosciences,buffer
R19054 T27602 T27601 punct ),Biosciences
R19055 T27603 T27597 cc and,stripping
R19056 T27604 T27597 conj reprobed,stripping
R19057 T27605 T27604 prep with,reprobed
R19058 T27606 T27607 amod new,antibodies
R19059 T27607 T27605 pobj antibodies,with
R19060 T27608 T27594 punct .,stripped
R18982 T27530 T27529 cc or,milk

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T27332 848-851 Protein denotes BSA
T27331 833-846 Protein denotes serum albumin
T27330 340-344 Protein denotes TNFα
T27329 310-321 Protein denotes µM zVAD.fmk
T27328 181-185 Protein denotes TNFα
T27327 151-162 Protein denotes µM zVAD.fmk

test3

Id Subject Object Predicate Lexical cue
T27058 340-344 Protein denotes TNFα
T27057 181-185 Protein denotes TNFα
T27056 340-344 Protein denotes TNFα
T27055 181-185 Protein denotes TNFα