PMC:3585731 / 39365-39989 JSONTXT

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However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T18398","span":{"begin":486,"end":490},"obj":"Protein"},{"id":"T18397","span":{"begin":455,"end":468},"obj":"Protein"},{"id":"T18396","span":{"begin":214,"end":218},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19386","span":{"begin":214,"end":218},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T19381","span":{"begin":550,"end":553},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T19380","span":{"begin":318,"end":321},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T19379","span":{"begin":48,"end":51},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T19337","span":{"begin":550,"end":553},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T19336","span":{"begin":318,"end":321},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T19335","span":{"begin":48,"end":51},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T19359","span":{"begin":550,"end":553},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T19358","span":{"begin":318,"end":321},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T19357","span":{"begin":48,"end":51},"obj":"http://www.uniprot.org/uniprot/P31751"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"GO-BP","denotations":[{"id":"T18470","span":{"begin":486,"end":490},"obj":"http://purl.obolibrary.org/obo/GO_0004740"},{"id":"T18469","span":{"begin":472,"end":476},"obj":"http://purl.obolibrary.org/obo/GO_0016303"},{"id":"T18465","span":{"begin":194,"end":199},"obj":"http://purl.obolibrary.org/obo/GO_0016265"},{"id":"T18449","span":{"begin":554,"end":569},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T18448","span":{"begin":292,"end":307},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T18447","span":{"begin":52,"end":67},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T18416","span":{"begin":124,"end":127},"obj":"http://purl.obolibrary.org/obo/GO_0004705"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"GO-MF","denotations":[{"id":"T18473","span":{"begin":124,"end":127},"obj":"http://purl.obolibrary.org/obo/GO_0004705"},{"id":"T18480","span":{"begin":486,"end":490},"obj":"http://purl.obolibrary.org/obo/GO_0004740"},{"id":"T18479","span":{"begin":472,"end":476},"obj":"http://purl.obolibrary.org/obo/GO_0016303"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"GO-CC","denotations":[{"id":"T18485","span":{"begin":183,"end":188},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"sentences","denotations":[{"id":"T18326","span":{"begin":497,"end":624},"obj":"Sentence"},{"id":"T18325","span":{"begin":361,"end":496},"obj":"Sentence"},{"id":"T18324","span":{"begin":129,"end":360},"obj":"Sentence"},{"id":"T18323","span":{"begin":0,"end":128},"obj":"Sentence"},{"id":"T244","span":{"begin":0,"end":128},"obj":"Sentence"},{"id":"T245","span":{"begin":129,"end":360},"obj":"Sentence"},{"id":"T246","span":{"begin":361,"end":496},"obj":"Sentence"},{"id":"T247","span":{"begin":497,"end":624},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"simple1","denotations":[{"id":"T18501","span":{"begin":486,"end":490},"obj":"Protein"},{"id":"T18500","span":{"begin":455,"end":468},"obj":"Protein"},{"id":"T18499","span":{"begin":214,"end":218},"obj":"Protein"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T19300","span":{"begin":443,"end":450},"obj":"Negative_regulation"},{"id":"T19299","span":{"begin":204,"end":213},"obj":"Negative_regulation"},{"id":"T19298","span":{"begin":219,"end":229},"obj":"Gene_expression"},{"id":"T19278","span":{"begin":486,"end":490},"obj":"Protein"},{"id":"T19277","span":{"begin":455,"end":468},"obj":"Protein"},{"id":"T19276","span":{"begin":214,"end":218},"obj":"Protein"}],"relations":[{"id":"R12909","pred":"themeOf","subj":"T19276","obj":"T19298"},{"id":"R12910","pred":"themeOf","subj":"T19277","obj":"T19300"},{"id":"R12925","pred":"themeOf","subj":"T19298","obj":"T19299"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T19501","span":{"begin":486,"end":490},"obj":"Protein"},{"id":"T19500","span":{"begin":455,"end":468},"obj":"Protein"},{"id":"T19499","span":{"begin":214,"end":218},"obj":"Protein"},{"id":"T19522","span":{"begin":219,"end":229},"obj":"Gene_expression"},{"id":"T19521","span":{"begin":204,"end":213},"obj":"Negative_regulation"},{"id":"T19524","span":{"begin":424,"end":433},"obj":"Negative_regulation"},{"id":"T19523","span":{"begin":443,"end":450},"obj":"Negative_regulation"}],"relations":[{"id":"R12978","pred":"themeOf","subj":"T19499","obj":"T19522"},{"id":"R12979","pred":"themeOf","subj":"T19500","obj":"T19523"},{"id":"R12992","pred":"themeOf","subj":"T19522","obj":"T19521"},{"id":"R12993","pred":"themeOf","subj":"T19523","obj":"T19524"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

{"project":"DLUT931","denotations":[{"id":"T19432","span":{"begin":219,"end":229},"obj":"Gene_expression"},{"id":"T19409","span":{"begin":486,"end":490},"obj":"Protein"},{"id":"T19408","span":{"begin":455,"end":468},"obj":"Protein"},{"id":"T19407","span":{"begin":214,"end":218},"obj":"Protein"},{"id":"T19433","span":{"begin":204,"end":213},"obj":"Negative_regulation"}],"relations":[{"id":"R12938","pred":"themeOf","subj":"T19407","obj":"T19432"},{"id":"R12948","pred":"themeOf","subj":"T19432","obj":"T19433"}],"text":"In order to further test our model, we examined Akt phosphorylation after inhibition of a downstream kinase in the pathway, JNK. However, we found that SP600125, which protected L929 cells from death and inhibited TNFα production (Fig. 2A,B S2A S10A), inhibited both basal and post-treatment phosphorylation levels of Akt at both Ser473 and Thr308 (Fig. S10B). It has been published that SP600125 is a somewhat non-specific inhibitor that may inhibit the p110δ subunit of PI3K [41] and PDK1 [42]. Both of these off-target effects could inhibit basal Akt phosphorylation levels, precluding the use of SP600125 in this system."}

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