PMC:3585731 / 29720-30779
Annnotations
testone
{"project":"testone","denotations":[{"id":"T31168","span":{"begin":774,"end":784},"obj":"Gene_expression"},{"id":"T31166","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31165","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31164","span":{"begin":677,"end":681},"obj":"Protein"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
pmc-enju-pas
{"project":"pmc-enju-pas","denotations":[{"id":"T31429","span":{"begin":1051,"end":1058},"obj":"VB"},{"id":"T31428","span":{"begin":1047,"end":1050},"obj":"VB"},{"id":"T31427","span":{"begin":1036,"end":1046},"obj":"NN"},{"id":"T31426","span":{"begin":1034,"end":1035},"obj":"-COMMA-"},{"id":"T31425","span":{"begin":1028,"end":1034},"obj":"NN"},{"id":"T31424","span":{"begin":1024,"end":1027},"obj":"DT"},{"id":"T31423","span":{"begin":1021,"end":1023},"obj":"IN"},{"id":"T31422","span":{"begin":1018,"end":1019},"obj":"-RRB-"},{"id":"T31421","span":{"begin":1017,"end":1018},"obj":"NN"},{"id":"T31420","span":{"begin":1016,"end":1017},"obj":"-LRB-"},{"id":"T31419","span":{"begin":1012,"end":1015},"obj":"NN"},{"id":"T31418","span":{"begin":1010,"end":1011},"obj":"CD"},{"id":"T31417","span":{"begin":1007,"end":1009},"obj":"IN"},{"id":"T31416","span":{"begin":999,"end":1006},"obj":"NN"},{"id":"T31415","span":{"begin":996,"end":998},"obj":"IN"},{"id":"T31414","span":{"begin":989,"end":995},"obj":"NN"},{"id":"T31413","span":{"begin":984,"end":988},"obj":"NN"},{"id":"T31412","span":{"begin":979,"end":983},"obj":"NN"},{"id":"T31411","span":{"begin":976,"end":978},"obj":"IN"},{"id":"T31410","span":{"begin":965,"end":975},"obj":"NN"},{"id":"T31409","span":{"begin":962,"end":964},"obj":"CC"},{"id":"T31408","span":{"begin":960,"end":961},"obj":"-COMMA-"},{"id":"T31407","span":{"begin":959,"end":960},"obj":"-RRB-"},{"id":"T31406","span":{"begin":958,"end":959},"obj":"NN"},{"id":"T31405","span":{"begin":957,"end":958},"obj":"-LRB-"},{"id":"T31404","span":{"begin":954,"end":956},"obj":"NN"},{"id":"T31403","span":{"begin":952,"end":953},"obj":"CD"},{"id":"T31402","span":{"begin":949,"end":951},"obj":"IN"},{"id":"T31401","span":{"begin":944,"end":948},"obj":"NN"},{"id":"T31400","span":{"begin":936,"end":943},"obj":"JJ"},{"id":"T31399","span":{"begin":934,"end":935},"obj":"-COMMA-"},{"id":"T31398","span":{"begin":933,"end":934},"obj":"-RRB-"},{"id":"T31397","span":{"begin":932,"end":933},"obj":"NN"},{"id":"T31396","span":{"begin":931,"end":932},"obj":"-LRB-"},{"id":"T31395","span":{"begin":928,"end":930},"obj":"NN"},{"id":"T31394","span":{"begin":925,"end":927},"obj":"CD"},{"id":"T31393","span":{"begin":922,"end":924},"obj":"IN"},{"id":"T31392","span":{"begin":916,"end":921},"obj":"NN"},{"id":"T31391","span":{"begin":906,"end":915},"obj":"NN"},{"id":"T31390","span":{"begin":903,"end":905},"obj":"IN"},{"id":"T31389","span":{"begin":894,"end":902},"obj":"VB"},{"id":"T31388","span":{"begin":892,"end":893},"obj":"-COMMA-"},{"id":"T31387","span":{"begin":882,"end":892},"obj":"NN"},{"id":"T31386","span":{"begin":877,"end":881},"obj":"JJ"},{"id":"T31385","span":{"begin":871,"end":876},"obj":"NN"},{"id":"T31384","span":{"begin":865,"end":870},"obj":"IN"},{"id":"T31383","span":{"begin":856,"end":864},"obj":"NN"},{"id":"T31382","span":{"begin":851,"end":855},"obj":"IN"},{"id":"T31381","span":{"begin":843,"end":850},"obj":"VB"},{"id":"T31380","span":{"begin":838,"end":842},"obj":"VB"},{"id":"T31379","span":{"begin":831,"end":837},"obj":"NN"},{"id":"T31378","span":{"begin":827,"end":830},"obj":"CC"},{"id":"T31377","span":{"begin":820,"end":826},"obj":"NN"},{"id":"T31376","span":{"begin":817,"end":819},"obj":"IN"},{"id":"T31375","span":{"begin":809,"end":816},"obj":"NN"},{"id":"T31374","span":{"begin":805,"end":808},"obj":"NN"},{"id":"T31373","span":{"begin":801,"end":804},"obj":"CC"},{"id":"T31372","span":{"begin":797,"end":800},"obj":"NN"},{"id":"T31371","span":{"begin":793,"end":796},"obj":"CC"},{"id":"T31370","span":{"begin":785,"end":792},"obj":"NN"},{"id":"T31369","span":{"begin":774,"end":784},"obj":"VB"},{"id":"T31368","span":{"begin":768,"end":773},"obj":"NN"},{"id":"T31367","span":{"begin":763,"end":767},"obj":"NN"},{"id":"T31366","span":{"begin":761,"end":762},"obj":"-RRB-"},{"id":"T31365","span":{"begin":758,"end":761},"obj":"NN"},{"id":"T31364","span":{"begin":757,"end":758},"obj":"-LRB-"},{"id":"T31363","span":{"begin":752,"end":756},"obj":"NN"},{"id":"T31362","span":{"begin":748,"end":751},"obj":"NN"},{"id":"T31361","span":{"begin":742,"end":747},"obj":"NN"},{"id":"T31360","span":{"begin":736,"end":741},"obj":"VB"},{"id":"T31359","span":{"begin":725,"end":735},"obj":"VB"},{"id":"T31358","span":{"begin":721,"end":724},"obj":"CC"},{"id":"T31357","span":{"begin":713,"end":720},"obj":"NN"},{"id":"T31356","span":{"begin":710,"end":712},"obj":"IN"},{"id":"T31355","span":{"begin":699,"end":709},"obj":"VB"},{"id":"T31354","span":{"begin":694,"end":698},"obj":"VB"},{"id":"T31353","span":{"begin":687,"end":693},"obj":"NN"},{"id":"T31352","span":{"begin":682,"end":686},"obj":"NN"},{"id":"T31351","span":{"begin":677,"end":681},"obj":"NN"},{"id":"T31350","span":{"begin":665,"end":675},"obj":"NN"},{"id":"T31349","span":{"begin":660,"end":664},"obj":"JJ"},{"id":"T31348","span":{"begin":654,"end":659},"obj":"NN"},{"id":"T31347","span":{"begin":648,"end":653},"obj":"IN"},{"id":"T31346","span":{"begin":645,"end":647},"obj":"NN"},{"id":"T31345","span":{"begin":643,"end":644},"obj":"CD"},{"id":"T31344","span":{"begin":639,"end":642},"obj":"IN"},{"id":"T31343","span":{"begin":630,"end":638},"obj":"NN"},{"id":"T31342","span":{"begin":625,"end":629},"obj":"IN"},{"id":"T31341","span":{"begin":614,"end":624},"obj":"VB"},{"id":"T31340","span":{"begin":609,"end":613},"obj":"VB"},{"id":"T31339","span":{"begin":607,"end":608},"obj":"-COMMA-"},{"id":"T31338","span":{"begin":601,"end":607},"obj":"NN"},{"id":"T31337","span":{"begin":593,"end":600},"obj":"NN"},{"id":"T31336","span":{"begin":589,"end":592},"obj":"CC"},{"id":"T31335","span":{"begin":581,"end":588},"obj":"NN"},{"id":"T31334","span":{"begin":576,"end":580},"obj":"IN"},{"id":"T31333","span":{"begin":567,"end":575},"obj":"VB"},{"id":"T31332","span":{"begin":560,"end":566},"obj":"RB"},{"id":"T31331","span":{"begin":558,"end":559},"obj":"-COMMA-"},{"id":"T31330","span":{"begin":553,"end":558},"obj":"NN"},{"id":"T31329","span":{"begin":548,"end":552},"obj":"NN"},{"id":"T31328","span":{"begin":546,"end":547},"obj":"-RRB-"},{"id":"T31327","span":{"begin":545,"end":546},"obj":"NN"},{"id":"T31326","span":{"begin":544,"end":545},"obj":"-LRB-"},{"id":"T31325","span":{"begin":533,"end":543},"obj":"VB"},{"id":"T31324","span":{"begin":529,"end":532},"obj":"VB"},{"id":"T31323","span":{"begin":523,"end":528},"obj":"NN"},{"id":"T31322","span":{"begin":521,"end":522},"obj":"-RRB-"},{"id":"T31321","span":{"begin":520,"end":521},"obj":"SYM"},{"id":"T31320","span":{"begin":519,"end":520},"obj":"-LRB-"},{"id":"T31279","span":{"begin":294,"end":303},"obj":"VB"},{"id":"T31278","span":{"begin":289,"end":293},"obj":"VB"},{"id":"T31277","span":{"begin":282,"end":288},"obj":"NN"},{"id":"T31276","span":{"begin":272,"end":281},"obj":"NN"},{"id":"T31275","span":{"begin":269,"end":270},"obj":"-RRB-"},{"id":"T31274","span":{"begin":268,"end":269},"obj":"NN"},{"id":"T31273","span":{"begin":267,"end":268},"obj":"-LRB-"},{"id":"T31272","span":{"begin":261,"end":266},"obj":"NN"},{"id":"T31271","span":{"begin":256,"end":260},"obj":"IN"},{"id":"T31270","span":{"begin":245,"end":255},"obj":"NN"},{"id":"T31269","span":{"begin":240,"end":244},"obj":"JJ"},{"id":"T31268","span":{"begin":234,"end":239},"obj":"NN"},{"id":"T31267","span":{"begin":231,"end":233},"obj":"CC"},{"id":"T31266","span":{"begin":225,"end":230},"obj":"NN"},{"id":"T31265","span":{"begin":222,"end":224},"obj":"CC"},{"id":"T31264","span":{"begin":220,"end":221},"obj":"-RRB-"},{"id":"T31263","span":{"begin":219,"end":220},"obj":"NN"},{"id":"T31262","span":{"begin":218,"end":219},"obj":"-LRB-"},{"id":"T31261","span":{"begin":207,"end":217},"obj":"NN"},{"id":"T31260","span":{"begin":202,"end":206},"obj":"JJ"},{"id":"T31259","span":{"begin":196,"end":201},"obj":"NN"},{"id":"T31258","span":{"begin":190,"end":195},"obj":"IN"},{"id":"T31257","span":{"begin":181,"end":189},"obj":"NN"},{"id":"T31256","span":{"begin":178,"end":180},"obj":"IN"},{"id":"T31255","span":{"begin":169,"end":177},"obj":"NN"},{"id":"T31254","span":{"begin":165,"end":168},"obj":"DT"},{"id":"T31253","span":{"begin":162,"end":164},"obj":"IN"},{"id":"T31252","span":{"begin":154,"end":161},"obj":"VB"},{"id":"T31251","span":{"begin":150,"end":153},"obj":"VB"},{"id":"T31250","span":{"begin":138,"end":149},"obj":"NN"},{"id":"T31249","span":{"begin":131,"end":136},"obj":"NN"},{"id":"T31248","span":{"begin":123,"end":130},"obj":"NN"},{"id":"T31247","span":{"begin":114,"end":122},"obj":"JJ"},{"id":"T31246","span":{"begin":100,"end":113},"obj":"RB"},{"id":"T31245","span":{"begin":96,"end":99},"obj":"DT"},{"id":"T31244","span":{"begin":93,"end":95},"obj":"CC"},{"id":"T31243","span":{"begin":85,"end":92},"obj":"NN"},{"id":"T31242","span":{"begin":76,"end":84},"obj":"VB"},{"id":"T31241","span":{"begin":68,"end":75},"obj":"NN"},{"id":"T31240","span":{"begin":65,"end":67},"obj":"CC"},{"id":"T31239","span":{"begin":54,"end":64},"obj":"NN"},{"id":"T31238","span":{"begin":49,"end":53},"obj":"NN"},{"id":"T31237","span":{"begin":43,"end":48},"obj":"JJ"},{"id":"T31236","span":{"begin":38,"end":42},"obj":"IN"},{"id":"T31235","span":{"begin":29,"end":37},"obj":"VB"},{"id":"T31234","span":{"begin":22,"end":28},"obj":"RB"},{"id":"T31233","span":{"begin":17,"end":21},"obj":"VB"},{"id":"T31232","span":{"begin":11,"end":16},"obj":"NN"},{"id":"T31231","span":{"begin":6,"end":10},"obj":"NN"},{"id":"T31230","span":{"begin":4,"end":5},"obj":"-RRB-"},{"id":"T31229","span":{"begin":3,"end":4},"obj":"NN"},{"id":"T31228","span":{"begin":2,"end":3},"obj":"-COMMA-"},{"id":"T31227","span":{"begin":1,"end":2},"obj":"NN"},{"id":"T31226","span":{"begin":0,"end":1},"obj":"-LRB-"},{"id":"T31319","span":{"begin":511,"end":518},"obj":"NN"},{"id":"T31318","span":{"begin":507,"end":510},"obj":"CC"},{"id":"T31317","span":{"begin":505,"end":506},"obj":"-RRB-"},{"id":"T31316","span":{"begin":504,"end":505},"obj":"NN"},{"id":"T31315","span":{"begin":503,"end":504},"obj":"-LRB-"},{"id":"T31314","span":{"begin":499,"end":502},"obj":"NN"},{"id":"T31313","span":{"begin":488,"end":498},"obj":"JJ"},{"id":"T31312","span":{"begin":476,"end":486},"obj":"NN"},{"id":"T31311","span":{"begin":466,"end":475},"obj":"JJ"},{"id":"T31310","span":{"begin":462,"end":465},"obj":"DT"},{"id":"T31309","span":{"begin":456,"end":461},"obj":"VB"},{"id":"T31308","span":{"begin":451,"end":455},"obj":"NN"},{"id":"T31307","span":{"begin":443,"end":450},"obj":"JJ"},{"id":"T31306","span":{"begin":440,"end":442},"obj":"IN"},{"id":"T31305","span":{"begin":431,"end":439},"obj":"VB"},{"id":"T31304","span":{"begin":429,"end":430},"obj":"-COMMA-"},{"id":"T31303","span":{"begin":427,"end":429},"obj":"NN"},{"id":"T31302","span":{"begin":425,"end":426},"obj":"CD"},{"id":"T31301","span":{"begin":421,"end":424},"obj":"IN"},{"id":"T31300","span":{"begin":410,"end":420},"obj":"NN"},{"id":"T31299","span":{"begin":405,"end":409},"obj":"JJ"},{"id":"T31298","span":{"begin":399,"end":404},"obj":"NN"},{"id":"T31297","span":{"begin":393,"end":398},"obj":"IN"},{"id":"T31296","span":{"begin":387,"end":392},"obj":"NN"},{"id":"T31295","span":{"begin":380,"end":386},"obj":"CC"},{"id":"T31294","span":{"begin":371,"end":379},"obj":"NN"},{"id":"T31293","span":{"begin":366,"end":370},"obj":"IN"},{"id":"T31292","span":{"begin":358,"end":365},"obj":"VB"},{"id":"T31291","span":{"begin":353,"end":357},"obj":"VB"},{"id":"T31290","span":{"begin":347,"end":352},"obj":"NN"},{"id":"T31289","span":{"begin":341,"end":346},"obj":"NN"},{"id":"T31288","span":{"begin":333,"end":340},"obj":"JJ"},{"id":"T31287","span":{"begin":329,"end":332},"obj":"CC"},{"id":"T31286","span":{"begin":321,"end":328},"obj":"NN"},{"id":"T31285","span":{"begin":319,"end":320},"obj":"-RRB-"},{"id":"T31284","span":{"begin":318,"end":319},"obj":"NN"},{"id":"T31283","span":{"begin":317,"end":318},"obj":"-LRB-"},{"id":"T31282","span":{"begin":313,"end":316},"obj":"NN"},{"id":"T31281","span":{"begin":310,"end":312},"obj":"CD"},{"id":"T31280","span":{"begin":304,"end":309},"obj":"IN"}],"relations":[{"id":"R21552","pred":"arg2Of","subj":"T31227","obj":"T31226"},{"id":"R21553","pred":"arg1Of","subj":"T31227","obj":"T31228"},{"id":"R21554","pred":"arg2Of","subj":"T31229","obj":"T31228"},{"id":"R21555","pred":"arg3Of","subj":"T31230","obj":"T31226"},{"id":"R21556","pred":"arg1Of","subj":"T31232","obj":"T31226"},{"id":"R21557","pred":"arg1Of","subj":"T31232","obj":"T31231"},{"id":"R21558","pred":"arg1Of","subj":"T31232","obj":"T31233"},{"id":"R21559","pred":"arg2Of","subj":"T31232","obj":"T31235"},{"id":"R21560","pred":"arg2Of","subj":"T31235","obj":"T31233"},{"id":"R21561","pred":"arg1Of","subj":"T31235","obj":"T31234"},{"id":"R21562","pred":"arg1Of","subj":"T31235","obj":"T31236"},{"id":"R21563","pred":"arg1Of","subj":"T31239","obj":"T31237"},{"id":"R21564","pred":"arg1Of","subj":"T31239","obj":"T31238"},{"id":"R21565","pred":"arg1Of","subj":"T31239","obj":"T31240"},{"id":"R21566","pred":"arg2Of","subj":"T31240","obj":"T31236"},{"id":"R21567","pred":"arg2Of","subj":"T31241","obj":"T31240"},{"id":"R21568","pred":"arg1Of","subj":"T31241","obj":"T31242"},{"id":"R21569","pred":"arg1Of","subj":"T31243","obj":"T31244"},{"id":"R21570","pred":"arg2Of","subj":"T31244","obj":"T31242"},{"id":"R21571","pred":"arg2Of","subj":"T31249","obj":"T31244"},{"id":"R21572","pred":"arg1Of","subj":"T31249","obj":"T31245"},{"id":"R21573","pred":"arg1Of","subj":"T31249","obj":"T31246"},{"id":"R21574","pred":"arg1Of","subj":"T31249","obj":"T31247"},{"id":"R21575","pred":"arg1Of","subj":"T31249","obj":"T31248"},{"id":"R21576","pred":"arg1Of","subj":"T31250","obj":"T31251"},{"id":"R21577","pred":"arg2Of","subj":"T31250","obj":"T31252"},{"id":"R21578","pred":"arg2Of","subj":"T31252","obj":"T31251"},{"id":"R21579","pred":"arg1Of","subj":"T31255","obj":"T31254"},{"id":"R21580","pred":"arg1Of","subj":"T31255","obj":"T31256"},{"id":"R21581","pred":"arg1Of","subj":"T31255","obj":"T31258"},{"id":"R21582","pred":"arg1Of","subj":"T31255","obj":"T31265"},{"id":"R21583","pred":"arg2Of","subj":"T31257","obj":"T31256"},{"id":"R21584","pred":"arg2Of","subj":"T31261","obj":"T31258"},{"id":"R21585","pred":"arg1Of","subj":"T31261","obj":"T31259"},{"id":"R21586","pred":"arg1Of","subj":"T31261","obj":"T31260"},{"id":"R21587","pred":"arg1Of","subj":"T31261","obj":"T31262"},{"id":"R21588","pred":"arg2Of","subj":"T31263","obj":"T31262"},{"id":"R21589","pred":"arg3Of","subj":"T31264","obj":"T31262"},{"id":"R21590","pred":"arg1Of","subj":"T31265","obj":"T31252"},{"id":"R21591","pred":"arg2Of","subj":"T31265","obj":"T31253"},{"id":"R21592","pred":"arg1Of","subj":"T31266","obj":"T31267"},{"id":"R21593","pred":"arg2Of","subj":"T31267","obj":"T31265"},{"id":"R21594","pred":"arg1Of","subj":"T31267","obj":"T31271"},{"id":"R21595","pred":"arg2Of","subj":"T31270","obj":"T31267"},{"id":"R21596","pred":"arg1Of","subj":"T31270","obj":"T31268"},{"id":"R21597","pred":"arg1Of","subj":"T31270","obj":"T31269"},{"id":"R21598","pred":"arg2Of","subj":"T31272","obj":"T31271"},{"id":"R21599","pred":"arg1Of","subj":"T31272","obj":"T31273"},{"id":"R21600","pred":"arg2Of","subj":"T31274","obj":"T31273"},{"id":"R21601","pred":"arg3Of","subj":"T31275","obj":"T31273"},{"id":"R21602","pred":"arg1Of","subj":"T31277","obj":"T31276"},{"id":"R21603","pred":"arg1Of","subj":"T31277","obj":"T31278"},{"id":"R21604","pred":"arg2Of","subj":"T31277","obj":"T31279"},{"id":"R21605","pred":"arg2Of","subj":"T31279","obj":"T31278"},{"id":"R21606","pred":"arg1Of","subj":"T31279","obj":"T31280"},{"id":"R21607","pred":"arg2Of","subj":"T31282","obj":"T31280"},{"id":"R21608","pred":"arg1Of","subj":"T31282","obj":"T31281"},{"id":"R21609","pred":"arg1Of","subj":"T31282","obj":"T31283"},{"id":"R21610","pred":"modOf","subj":"T31282","obj":"T31291"},{"id":"R21611","pred":"arg2Of","subj":"T31284","obj":"T31283"},{"id":"R21612","pred":"arg3Of","subj":"T31285","obj":"T31283"},{"id":"R21613","pred":"arg1Of","subj":"T31286","obj":"T31287"},{"id":"R21614","pred":"arg1Of","subj":"T31287","obj":"T31291"},{"id":"R21615","pred":"arg2Of","subj":"T31287","obj":"T31292"},{"id":"R21616","pred":"arg2Of","subj":"T31290","obj":"T31287"},{"id":"R21617","pred":"arg1Of","subj":"T31290","obj":"T31288"},{"id":"R21618","pred":"arg1Of","subj":"T31290","obj":"T31289"},{"id":"R21619","pred":"arg2Of","subj":"T31292","obj":"T31291"},{"id":"R21620","pred":"arg1Of","subj":"T31292","obj":"T31293"},{"id":"R21621","pred":"arg1Of","subj":"T31292","obj":"T31297"},{"id":"R21622","pred":"arg1Of","subj":"T31292","obj":"T31301"},{"id":"R21623","pred":"arg1Of","subj":"T31292","obj":"T31304"},{"id":"R21624","pred":"modOf","subj":"T31292","obj":"T31305"},{"id":"R21625","pred":"arg1Of","subj":"T31294","obj":"T31295"},{"id":"R21626","pred":"arg2Of","subj":"T31295","obj":"T31293"},{"id":"R21627","pred":"arg2Of","subj":"T31296","obj":"T31295"},{"id":"R21628","pred":"arg2Of","subj":"T31300","obj":"T31297"},{"id":"R21629","pred":"arg1Of","subj":"T31300","obj":"T31298"},{"id":"R21630","pred":"arg1Of","subj":"T31300","obj":"T31299"},{"id":"R21631","pred":"arg2Of","subj":"T31303","obj":"T31301"},{"id":"R21632","pred":"arg1Of","subj":"T31303","obj":"T31302"},{"id":"R21633","pred":"arg1Of","subj":"T31308","obj":"T31305"},{"id":"R21634","pred":"arg2Of","subj":"T31308","obj":"T31306"},{"id":"R21635","pred":"arg1Of","subj":"T31308","obj":"T31307"},{"id":"R21636","pred":"arg1Of","subj":"T31308","obj":"T31309"},{"id":"R21637","pred":"arg2Of","subj":"T31312","obj":"T31309"},{"id":"R21638","pred":"arg1Of","subj":"T31312","obj":"T31310"},{"id":"R21639","pred":"arg1Of","subj":"T31312","obj":"T31311"},{"id":"R21640","pred":"arg1Of","subj":"T31314","obj":"T31313"},{"id":"R21641","pred":"arg1Of","subj":"T31314","obj":"T31315"},{"id":"R21642","pred":"arg1Of","subj":"T31314","obj":"T31318"},{"id":"R21643","pred":"arg2Of","subj":"T31316","obj":"T31315"},{"id":"R21644","pred":"arg3Of","subj":"T31317","obj":"T31315"},{"id":"R21645","pred":"modOf","subj":"T31318","obj":"T31324"},{"id":"R21646","pred":"arg1Of","subj":"T31318","obj":"T31340"},{"id":"R21647","pred":"arg2Of","subj":"T31318","obj":"T31341"},{"id":"R21648","pred":"arg2Of","subj":"T31319","obj":"T31318"},{"id":"R21649","pred":"arg1Of","subj":"T31319","obj":"T31320"},{"id":"R21650","pred":"arg2Of","subj":"T31321","obj":"T31320"},{"id":"R21651","pred":"arg3Of","subj":"T31322","obj":"T31320"},{"id":"R21652","pred":"arg1Of","subj":"T31323","obj":"T31324"},{"id":"R21653","pred":"arg2Of","subj":"T31323","obj":"T31325"},{"id":"R21654","pred":"arg2Of","subj":"T31325","obj":"T31324"},{"id":"R21655","pred":"arg2Of","subj":"T31327","obj":"T31326"},{"id":"R21656","pred":"arg3Of","subj":"T31328","obj":"T31326"},{"id":"R21657","pred":"arg3Of","subj":"T31330","obj":"T31325"},{"id":"R21658","pred":"arg1Of","subj":"T31330","obj":"T31326"},{"id":"R21659","pred":"arg1Of","subj":"T31330","obj":"T31329"},{"id":"R21660","pred":"arg1Of","subj":"T31330","obj":"T31331"},{"id":"R21661","pred":"arg2Of","subj":"T31330","obj":"T31333"},{"id":"R21662","pred":"arg1Of","subj":"T31333","obj":"T31332"},{"id":"R21663","pred":"arg1Of","subj":"T31333","obj":"T31334"},{"id":"R21664","pred":"arg1Of","subj":"T31335","obj":"T31336"},{"id":"R21665","pred":"arg2Of","subj":"T31336","obj":"T31334"},{"id":"R21666","pred":"arg2Of","subj":"T31338","obj":"T31336"},{"id":"R21667","pred":"arg1Of","subj":"T31338","obj":"T31337"},{"id":"R21668","pred":"arg1Of","subj":"T31341","obj":"T31339"},{"id":"R21669","pred":"arg2Of","subj":"T31341","obj":"T31340"},{"id":"R21670","pred":"arg1Of","subj":"T31341","obj":"T31342"},{"id":"R21671","pred":"arg1Of","subj":"T31341","obj":"T31344"},{"id":"R21672","pred":"arg1Of","subj":"T31341","obj":"T31347"},{"id":"R21673","pred":"arg2Of","subj":"T31343","obj":"T31342"},{"id":"R21674","pred":"arg2Of","subj":"T31346","obj":"T31344"},{"id":"R21675","pred":"arg1Of","subj":"T31346","obj":"T31345"},{"id":"R21676","pred":"arg2Of","subj":"T31350","obj":"T31347"},{"id":"R21677","pred":"arg1Of","subj":"T31350","obj":"T31348"},{"id":"R21678","pred":"arg1Of","subj":"T31350","obj":"T31349"},{"id":"R21679","pred":"arg1Of","subj":"T31353","obj":"T31351"},{"id":"R21680","pred":"arg1Of","subj":"T31353","obj":"T31352"},{"id":"R21681","pred":"arg1Of","subj":"T31353","obj":"T31354"},{"id":"R21682","pred":"arg2Of","subj":"T31353","obj":"T31355"},{"id":"R21683","pred":"arg2Of","subj":"T31353","obj":"T31359"},{"id":"R21684","pred":"arg1Of","subj":"T31353","obj":"T31360"},{"id":"R21685","pred":"arg1Of","subj":"T31355","obj":"T31356"},{"id":"R21686","pred":"arg1Of","subj":"T31355","obj":"T31358"},{"id":"R21687","pred":"arg2Of","subj":"T31357","obj":"T31356"},{"id":"R21688","pred":"arg2Of","subj":"T31358","obj":"T31354"},{"id":"R21689","pred":"arg2Of","subj":"T31359","obj":"T31358"},{"id":"R21690","pred":"arg3Of","subj":"T31360","obj":"T31359"},{"id":"R21691","pred":"arg1Of","subj":"T31360","obj":"T31415"},{"id":"R21692","pred":"arg1Of","subj":"T31363","obj":"T31361"},{"id":"R21693","pred":"arg1Of","subj":"T31363","obj":"T31362"},{"id":"R21694","pred":"arg1Of","subj":"T31363","obj":"T31364"},{"id":"R21695","pred":"modOf","subj":"T31363","obj":"T31380"},{"id":"R21696","pred":"arg1Of","subj":"T31363","obj":"T31399"},{"id":"R21697","pred":"arg2Of","subj":"T31365","obj":"T31364"},{"id":"R21698","pred":"arg3Of","subj":"T31366","obj":"T31364"},{"id":"R21699","pred":"arg1Of","subj":"T31368","obj":"T31367"},{"id":"R21700","pred":"arg1Of","subj":"T31368","obj":"T31369"},{"id":"R21701","pred":"arg1Of","subj":"T31368","obj":"T31380"},{"id":"R21702","pred":"arg2Of","subj":"T31368","obj":"T31381"},{"id":"R21703","pred":"arg1Of","subj":"T31370","obj":"T31371"},{"id":"R21704","pred":"arg1Of","subj":"T31371","obj":"T31373"},{"id":"R21705","pred":"arg2Of","subj":"T31372","obj":"T31371"},{"id":"R21706","pred":"arg2Of","subj":"T31373","obj":"T31369"},{"id":"R21707","pred":"arg2Of","subj":"T31375","obj":"T31373"},{"id":"R21708","pred":"arg1Of","subj":"T31375","obj":"T31374"},{"id":"R21709","pred":"arg1Of","subj":"T31375","obj":"T31376"},{"id":"R21710","pred":"arg1Of","subj":"T31377","obj":"T31378"},{"id":"R21711","pred":"arg2Of","subj":"T31378","obj":"T31376"},{"id":"R21712","pred":"arg2Of","subj":"T31379","obj":"T31378"},{"id":"R21713","pred":"arg2Of","subj":"T31381","obj":"T31380"},{"id":"R21714","pred":"arg1Of","subj":"T31381","obj":"T31382"},{"id":"R21715","pred":"arg1Of","subj":"T31381","obj":"T31384"},{"id":"R21716","pred":"arg1Of","subj":"T31381","obj":"T31388"},{"id":"R21717","pred":"modOf","subj":"T31381","obj":"T31389"},{"id":"R21718","pred":"arg2Of","subj":"T31383","obj":"T31382"},{"id":"R21719","pred":"arg2Of","subj":"T31387","obj":"T31384"},{"id":"R21720","pred":"arg1Of","subj":"T31387","obj":"T31385"},{"id":"R21721","pred":"arg1Of","subj":"T31387","obj":"T31386"},{"id":"R21722","pred":"arg1Of","subj":"T31392","obj":"T31389"},{"id":"R21723","pred":"arg2Of","subj":"T31392","obj":"T31390"},{"id":"R21724","pred":"arg1Of","subj":"T31392","obj":"T31391"},{"id":"R21725","pred":"arg1Of","subj":"T31392","obj":"T31393"},{"id":"R21726","pred":"arg2Of","subj":"T31395","obj":"T31393"},{"id":"R21727","pred":"arg1Of","subj":"T31395","obj":"T31394"},{"id":"R21728","pred":"arg1Of","subj":"T31395","obj":"T31396"},{"id":"R21729","pred":"arg2Of","subj":"T31397","obj":"T31396"},{"id":"R21730","pred":"arg3Of","subj":"T31398","obj":"T31396"},{"id":"R21731","pred":"arg1Of","subj":"T31399","obj":"T31409"},{"id":"R21732","pred":"arg2Of","subj":"T31401","obj":"T31399"},{"id":"R21733","pred":"arg1Of","subj":"T31401","obj":"T31400"},{"id":"R21734","pred":"arg1Of","subj":"T31401","obj":"T31402"},{"id":"R21735","pred":"arg2Of","subj":"T31404","obj":"T31402"},{"id":"R21736","pred":"arg1Of","subj":"T31404","obj":"T31403"},{"id":"R21737","pred":"arg1Of","subj":"T31404","obj":"T31405"},{"id":"R21738","pred":"arg2Of","subj":"T31406","obj":"T31405"},{"id":"R21739","pred":"arg3Of","subj":"T31407","obj":"T31405"},{"id":"R21740","pred":"arg2Of","subj":"T31409","obj":"T31360"},{"id":"R21741","pred":"arg1Of","subj":"T31409","obj":"T31408"},{"id":"R21742","pred":"arg2Of","subj":"T31410","obj":"T31409"},{"id":"R21743","pred":"arg1Of","subj":"T31410","obj":"T31411"},{"id":"R21744","pred":"arg2Of","subj":"T31414","obj":"T31411"},{"id":"R21745","pred":"arg1Of","subj":"T31414","obj":"T31412"},{"id":"R21746","pred":"arg1Of","subj":"T31414","obj":"T31413"},{"id":"R21747","pred":"arg2Of","subj":"T31416","obj":"T31415"},{"id":"R21748","pred":"arg1Of","subj":"T31416","obj":"T31417"},{"id":"R21749","pred":"arg2Of","subj":"T31419","obj":"T31417"},{"id":"R21750","pred":"arg1Of","subj":"T31419","obj":"T31418"},{"id":"R21751","pred":"arg1Of","subj":"T31419","obj":"T31420"},{"id":"R21752","pred":"arg2Of","subj":"T31421","obj":"T31420"},{"id":"R21753","pred":"arg3Of","subj":"T31422","obj":"T31420"},{"id":"R21754","pred":"arg2Of","subj":"T31425","obj":"T31423"},{"id":"R21755","pred":"arg1Of","subj":"T31425","obj":"T31424"},{"id":"R21756","pred":"arg1Of","subj":"T31427","obj":"T31428"},{"id":"R21757","pred":"arg2Of","subj":"T31427","obj":"T31429"},{"id":"R21758","pred":"arg1Of","subj":"T31429","obj":"T31423"},{"id":"R21759","pred":"arg1Of","subj":"T31429","obj":"T31426"},{"id":"R21760","pred":"arg2Of","subj":"T31429","obj":"T31428"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
bionlp-st-ge-2016-test-proteins
{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T31196","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31195","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31194","span":{"begin":677,"end":681},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T31465","span":{"begin":979,"end":983},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T31464","span":{"begin":677,"end":681},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T31463","span":{"begin":789,"end":792},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31462","span":{"begin":597,"end":600},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31461","span":{"begin":585,"end":588},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31460","span":{"begin":515,"end":518},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31459","span":{"begin":499,"end":502},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31458","span":{"begin":337,"end":340},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31457","span":{"begin":325,"end":328},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31456","span":{"begin":127,"end":130},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31455","span":{"begin":89,"end":92},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T31453","span":{"begin":789,"end":792},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31452","span":{"begin":597,"end":600},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31451","span":{"begin":585,"end":588},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31450","span":{"begin":515,"end":518},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31449","span":{"begin":499,"end":502},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31448","span":{"begin":337,"end":340},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31447","span":{"begin":325,"end":328},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31446","span":{"begin":127,"end":130},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31445","span":{"begin":89,"end":92},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T31443","span":{"begin":789,"end":792},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31442","span":{"begin":597,"end":600},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31441","span":{"begin":585,"end":588},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31440","span":{"begin":515,"end":518},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31439","span":{"begin":499,"end":502},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31438","span":{"begin":337,"end":340},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31437","span":{"begin":325,"end":328},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31436","span":{"begin":127,"end":130},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T31435","span":{"begin":89,"end":92},"obj":"http://www.uniprot.org/uniprot/P31749"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
UBERON-AE
{"project":"UBERON-AE","denotations":[{"id":"T31183","span":{"begin":871,"end":876},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T31182","span":{"begin":654,"end":659},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T31181","span":{"begin":399,"end":404},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T31180","span":{"begin":234,"end":239},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T31179","span":{"begin":225,"end":230},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T31178","span":{"begin":196,"end":201},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T31200","span":{"begin":138,"end":149},"obj":"http://purl.obolibrary.org/obo/GO_0097528"},{"id":"T31198","span":{"begin":138,"end":149},"obj":"http://purl.obolibrary.org/obo/GO_0070266"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T31201","span":{"begin":476,"end":486},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T31207","span":{"begin":476,"end":486},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T31206","span":{"begin":476,"end":486},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T31205","span":{"begin":768,"end":773},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T31204","span":{"begin":553,"end":558},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T31203","span":{"begin":347,"end":352},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T31202","span":{"begin":11,"end":16},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
sentences
{"project":"sentences","denotations":[{"id":"T31190","span":{"begin":1021,"end":1059},"obj":"Sentence"},{"id":"T31189","span":{"begin":677,"end":1020},"obj":"Sentence"},{"id":"T31188","span":{"begin":488,"end":676},"obj":"Sentence"},{"id":"T31187","span":{"begin":272,"end":487},"obj":"Sentence"},{"id":"T31186","span":{"begin":138,"end":271},"obj":"Sentence"},{"id":"T31185","span":{"begin":0,"end":137},"obj":"Sentence"},{"id":"T187","span":{"begin":0,"end":137},"obj":"Sentence"},{"id":"T188","span":{"begin":138,"end":271},"obj":"Sentence"},{"id":"T189","span":{"begin":272,"end":487},"obj":"Sentence"},{"id":"T190","span":{"begin":488,"end":676},"obj":"Sentence"},{"id":"T191","span":{"begin":677,"end":1020},"obj":"Sentence"},{"id":"T192","span":{"begin":1021,"end":1059},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
simple1
{"project":"simple1","denotations":[{"id":"T31213","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31212","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31211","span":{"begin":677,"end":681},"obj":"Protein"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
BioNLP16_DUT
{"project":"BioNLP16_DUT","denotations":[{"id":"T31433","span":{"begin":687,"end":693},"obj":"Transcription"},{"id":"T31432","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31431","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31430","span":{"begin":677,"end":681},"obj":"Protein"}],"relations":[{"id":"R21761","pred":"themeOf","subj":"T31430","obj":"T31433"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
BioNLP16_Messiy
{"project":"BioNLP16_Messiy","denotations":[{"id":"T31704","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31703","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31702","span":{"begin":677,"end":681},"obj":"Protein"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
DLUT931
{"project":"DLUT931","denotations":[{"id":"T31470","span":{"begin":989,"end":995},"obj":"Transcription"},{"id":"T31469","span":{"begin":687,"end":693},"obj":"Transcription"},{"id":"T31468","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31467","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31466","span":{"begin":677,"end":681},"obj":"Protein"}],"relations":[{"id":"R21762","pred":"themeOf","subj":"T31466","obj":"T31469"},{"id":"R21763","pred":"themeOf","subj":"T31468","obj":"T31470"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
bionlp-st-ge-2016-test-ihmc
{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T31780","span":{"begin":593,"end":608},"obj":"Entity"},{"id":"T31779","span":{"begin":976,"end":1006},"obj":"Protein"},{"id":"T31778","span":{"begin":321,"end":328},"obj":"Entity"},{"id":"T31777","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31776","span":{"begin":333,"end":352},"obj":"Entity"},{"id":"T31775","span":{"begin":742,"end":755},"obj":"Protein"},{"id":"T31774","span":{"begin":333,"end":352},"obj":"Protein"},{"id":"T31773","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31772","span":{"begin":785,"end":792},"obj":"Entity"},{"id":"T31771","span":{"begin":1010,"end":1019},"obj":"Protein"},{"id":"T31770","span":{"begin":677,"end":681},"obj":"Protein"},{"id":"T31769","span":{"begin":488,"end":506},"obj":"Protein"},{"id":"T31768","span":{"begin":958,"end":959},"obj":"Entity"},{"id":"T31767","span":{"begin":333,"end":352},"obj":"Entity"},{"id":"T31766","span":{"begin":831,"end":837},"obj":"Entity"},{"id":"T31765","span":{"begin":387,"end":429},"obj":"Protein"},{"id":"T31764","span":{"begin":114,"end":136},"obj":"Protein"},{"id":"T31763","span":{"begin":178,"end":189},"obj":"Entity"},{"id":"T31762","span":{"begin":763,"end":767},"obj":"Entity"},{"id":"T31761","span":{"begin":581,"end":588},"obj":"Protein"},{"id":"T31760","span":{"begin":511,"end":518},"obj":"Protein"},{"id":"T31759","span":{"begin":593,"end":608},"obj":"Entity"},{"id":"T31758","span":{"begin":677,"end":693},"obj":"Protein"},{"id":"T31757","span":{"begin":758,"end":761},"obj":"Entity"},{"id":"T31756","span":{"begin":763,"end":837},"obj":"Entity"},{"id":"T31755","span":{"begin":371,"end":379},"obj":"Entity"},{"id":"T31754","span":{"begin":114,"end":136},"obj":"Entity"},{"id":"T31753","span":{"begin":820,"end":826},"obj":"Entity"},{"id":"T31752","span":{"begin":856,"end":864},"obj":"Entity"},{"id":"T31751","span":{"begin":476,"end":486},"obj":"Entity"},{"id":"T31750","span":{"begin":593,"end":608},"obj":"Protein"},{"id":"T31749","span":{"begin":85,"end":92},"obj":"Entity"},{"id":"T31748","span":{"begin":511,"end":518},"obj":"Entity"},{"id":"T31747","span":{"begin":85,"end":92},"obj":"Protein"},{"id":"T31746","span":{"begin":581,"end":588},"obj":"Entity"},{"id":"T31745","span":{"begin":805,"end":808},"obj":"Protein"},{"id":"T31744","span":{"begin":999,"end":1006},"obj":"Protein"},{"id":"T31743","span":{"begin":261,"end":270},"obj":"Protein"},{"id":"T31742","span":{"begin":785,"end":792},"obj":"Protein"},{"id":"T31741","span":{"begin":630,"end":647},"obj":"Entity"},{"id":"T31740","span":{"begin":797,"end":800},"obj":"Entity"},{"id":"T31739","span":{"begin":321,"end":328},"obj":"Protein"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
bionlp-st-ge-2016-spacy-parsed
{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T31698","span":{"begin":1058,"end":1059},"obj":"."},{"id":"T31697","span":{"begin":1051,"end":1058},"obj":"VBN"},{"id":"T31696","span":{"begin":1047,"end":1050},"obj":"VBD"},{"id":"T31695","span":{"begin":1044,"end":1046},"obj":"NNP"},{"id":"T31694","span":{"begin":1043,"end":1044},"obj":"NN"},{"id":"T31693","span":{"begin":1036,"end":1043},"obj":"JJ"},{"id":"T31692","span":{"begin":1034,"end":1035},"obj":","},{"id":"T31691","span":{"begin":1028,"end":1034},"obj":"NNS"},{"id":"T31690","span":{"begin":1024,"end":1027},"obj":"DT"},{"id":"T31689","span":{"begin":1021,"end":1023},"obj":"IN"},{"id":"T31688","span":{"begin":1019,"end":1020},"obj":"."},{"id":"T31687","span":{"begin":1018,"end":1019},"obj":"-RRB-"},{"id":"T31686","span":{"begin":1017,"end":1018},"obj":"NNP"},{"id":"T31685","span":{"begin":1016,"end":1017},"obj":"-LRB-"},{"id":"T31684","span":{"begin":1012,"end":1015},"obj":"NNS"},{"id":"T31683","span":{"begin":1010,"end":1011},"obj":"CD"},{"id":"T31682","span":{"begin":1007,"end":1009},"obj":"IN"},{"id":"T31681","span":{"begin":999,"end":1006},"obj":"NNP"},{"id":"T31680","span":{"begin":996,"end":998},"obj":"IN"},{"id":"T31679","span":{"begin":989,"end":995},"obj":"NNS"},{"id":"T31678","span":{"begin":984,"end":988},"obj":"NNP"},{"id":"T31677","span":{"begin":979,"end":983},"obj":"NNP"},{"id":"T31676","span":{"begin":976,"end":978},"obj":"IN"},{"id":"T31675","span":{"begin":965,"end":975},"obj":"NN"},{"id":"T31674","span":{"begin":962,"end":964},"obj":"CC"},{"id":"T31673","span":{"begin":960,"end":961},"obj":","},{"id":"T31672","span":{"begin":959,"end":960},"obj":"-RRB-"},{"id":"T31671","span":{"begin":958,"end":959},"obj":"NN"},{"id":"T31670","span":{"begin":957,"end":958},"obj":"-LRB-"},{"id":"T31669","span":{"begin":954,"end":956},"obj":"NN"},{"id":"T31668","span":{"begin":952,"end":953},"obj":"CD"},{"id":"T31667","span":{"begin":949,"end":951},"obj":"IN"},{"id":"T31666","span":{"begin":944,"end":948},"obj":"NN"},{"id":"T31628","span":{"begin":755,"end":756},"obj":"."},{"id":"T31587","span":{"begin":533,"end":542},"obj":"VBN"},{"id":"T31586","span":{"begin":529,"end":532},"obj":"VBP"},{"id":"T31585","span":{"begin":523,"end":528},"obj":"NNS"},{"id":"T31584","span":{"begin":521,"end":522},"obj":"-RRB-"},{"id":"T31583","span":{"begin":520,"end":521},"obj":"SYM"},{"id":"T31582","span":{"begin":519,"end":520},"obj":"-LRB-"},{"id":"T31581","span":{"begin":511,"end":518},"obj":"NNP"},{"id":"T31580","span":{"begin":507,"end":510},"obj":"CC"},{"id":"T31579","span":{"begin":505,"end":506},"obj":"-RRB-"},{"id":"T31578","span":{"begin":504,"end":505},"obj":"VBN"},{"id":"T31535","span":{"begin":270,"end":271},"obj":"."},{"id":"T31534","span":{"begin":269,"end":270},"obj":"-RRB-"},{"id":"T31533","span":{"begin":268,"end":269},"obj":"NNP"},{"id":"T31532","span":{"begin":267,"end":268},"obj":"-LRB-"},{"id":"T31531","span":{"begin":261,"end":266},"obj":"NN"},{"id":"T31530","span":{"begin":256,"end":260},"obj":"IN"},{"id":"T31529","span":{"begin":245,"end":255},"obj":"NNS"},{"id":"T31528","span":{"begin":240,"end":244},"obj":"JJ"},{"id":"T31527","span":{"begin":234,"end":239},"obj":"NN"},{"id":"T31526","span":{"begin":231,"end":233},"obj":"CC"},{"id":"T31525","span":{"begin":225,"end":230},"obj":"NN"},{"id":"T31524","span":{"begin":222,"end":224},"obj":"CC"},{"id":"T31523","span":{"begin":220,"end":221},"obj":"-RRB-"},{"id":"T31522","span":{"begin":219,"end":220},"obj":"NNP"},{"id":"T31521","span":{"begin":218,"end":219},"obj":"-LRB-"},{"id":"T31520","span":{"begin":207,"end":217},"obj":"NNS"},{"id":"T31519","span":{"begin":202,"end":206},"obj":"JJ"},{"id":"T31518","span":{"begin":196,"end":201},"obj":"NN"},{"id":"T31517","span":{"begin":190,"end":195},"obj":"IN"},{"id":"T31516","span":{"begin":181,"end":189},"obj":"NNP"},{"id":"T31515","span":{"begin":178,"end":180},"obj":"IN"},{"id":"T31514","span":{"begin":169,"end":177},"obj":"NN"},{"id":"T31513","span":{"begin":165,"end":168},"obj":"DT"},{"id":"T31512","span":{"begin":162,"end":164},"obj":"IN"},{"id":"T31511","span":{"begin":154,"end":161},"obj":"VBN"},{"id":"T31510","span":{"begin":150,"end":153},"obj":"VBD"},{"id":"T31509","span":{"begin":138,"end":149},"obj":"NNP"},{"id":"T31508","span":{"begin":136,"end":137},"obj":"."},{"id":"T31507","span":{"begin":131,"end":136},"obj":"NNP"},{"id":"T31506","span":{"begin":123,"end":130},"obj":"NNP"},{"id":"T31505","span":{"begin":114,"end":122},"obj":"JJ"},{"id":"T31504","span":{"begin":100,"end":113},"obj":"RB"},{"id":"T31503","span":{"begin":96,"end":99},"obj":"DT"},{"id":"T31502","span":{"begin":93,"end":95},"obj":"CC"},{"id":"T31501","span":{"begin":85,"end":92},"obj":"NNP"},{"id":"T31500","span":{"begin":76,"end":84},"obj":"VBG"},{"id":"T31499","span":{"begin":68,"end":75},"obj":"NNS"},{"id":"T31498","span":{"begin":65,"end":67},"obj":"CC"},{"id":"T31497","span":{"begin":54,"end":64},"obj":"NN"},{"id":"T31496","span":{"begin":49,"end":53},"obj":"NNP"},{"id":"T31495","span":{"begin":43,"end":48},"obj":"JJ"},{"id":"T31494","span":{"begin":38,"end":42},"obj":"IN"},{"id":"T31493","span":{"begin":29,"end":37},"obj":"VBN"},{"id":"T31492","span":{"begin":22,"end":28},"obj":"RB"},{"id":"T31491","span":{"begin":17,"end":21},"obj":"VBD"},{"id":"T31577","span":{"begin":503,"end":504},"obj":"-LRB-"},{"id":"T31576","span":{"begin":499,"end":502},"obj":"NNP"},{"id":"T31575","span":{"begin":488,"end":498},"obj":"NNP"},{"id":"T31574","span":{"begin":486,"end":487},"obj":"."},{"id":"T31573","span":{"begin":476,"end":486},"obj":"NNS"},{"id":"T31572","span":{"begin":466,"end":475},"obj":"VBN"},{"id":"T31571","span":{"begin":462,"end":465},"obj":"DT"},{"id":"T31570","span":{"begin":456,"end":461},"obj":"VBG"},{"id":"T31569","span":{"begin":451,"end":455},"obj":"NN"},{"id":"T31568","span":{"begin":443,"end":450},"obj":"JJ"},{"id":"T31567","span":{"begin":440,"end":442},"obj":"IN"},{"id":"T31566","span":{"begin":431,"end":439},"obj":"VBN"},{"id":"T31565","span":{"begin":429,"end":430},"obj":","},{"id":"T31564","span":{"begin":427,"end":429},"obj":"NN"},{"id":"T31563","span":{"begin":425,"end":426},"obj":"CD"},{"id":"T31562","span":{"begin":421,"end":424},"obj":"IN"},{"id":"T31561","span":{"begin":410,"end":420},"obj":"NNS"},{"id":"T31560","span":{"begin":405,"end":409},"obj":"JJ"},{"id":"T31559","span":{"begin":399,"end":404},"obj":"NN"},{"id":"T31558","span":{"begin":393,"end":398},"obj":"IN"},{"id":"T31557","span":{"begin":387,"end":392},"obj":"NNP"},{"id":"T31556","span":{"begin":380,"end":386},"obj":"CC"},{"id":"T31555","span":{"begin":371,"end":379},"obj":"NNP"},{"id":"T31554","span":{"begin":366,"end":370},"obj":"IN"},{"id":"T31553","span":{"begin":358,"end":365},"obj":"VBN"},{"id":"T31552","span":{"begin":353,"end":357},"obj":"VBD"},{"id":"T31551","span":{"begin":347,"end":352},"obj":"NNS"},{"id":"T31550","span":{"begin":341,"end":346},"obj":"NNP"},{"id":"T31549","span":{"begin":333,"end":340},"obj":"NNP"},{"id":"T31548","span":{"begin":329,"end":332},"obj":"CC"},{"id":"T31547","span":{"begin":321,"end":328},"obj":"NNP"},{"id":"T31546","span":{"begin":319,"end":320},"obj":"-RRB-"},{"id":"T31545","span":{"begin":318,"end":319},"obj":"NNP"},{"id":"T31544","span":{"begin":317,"end":318},"obj":"-LRB-"},{"id":"T31543","span":{"begin":315,"end":316},"obj":"."},{"id":"T31542","span":{"begin":313,"end":315},"obj":"NN"},{"id":"T31541","span":{"begin":310,"end":312},"obj":"CD"},{"id":"T31540","span":{"begin":304,"end":309},"obj":"IN"},{"id":"T31539","span":{"begin":294,"end":303},"obj":"VBN"},{"id":"T31538","span":{"begin":289,"end":293},"obj":"VBD"},{"id":"T31537","span":{"begin":282,"end":288},"obj":"NNS"},{"id":"T31536","span":{"begin":272,"end":281},"obj":"NN"},{"id":"T31588","span":{"begin":542,"end":543},"obj":"."},{"id":"T31488","span":{"begin":4,"end":5},"obj":"-RRB-"},{"id":"T31487","span":{"begin":3,"end":4},"obj":"NNP"},{"id":"T31486","span":{"begin":2,"end":3},"obj":","},{"id":"T31485","span":{"begin":1,"end":2},"obj":"DT"},{"id":"T31484","span":{"begin":0,"end":1},"obj":"-LRB-"},{"id":"T31490","span":{"begin":11,"end":16},"obj":"NNS"},{"id":"T31489","span":{"begin":6,"end":10},"obj":"NNP"},{"id":"T31627","span":{"begin":752,"end":755},"obj":"NNP"},{"id":"T31626","span":{"begin":748,"end":751},"obj":"CD"},{"id":"T31625","span":{"begin":742,"end":747},"obj":"NN"},{"id":"T31624","span":{"begin":736,"end":741},"obj":"VBG"},{"id":"T31623","span":{"begin":725,"end":735},"obj":"VBN"},{"id":"T31622","span":{"begin":721,"end":724},"obj":"CC"},{"id":"T31621","span":{"begin":713,"end":720},"obj":"NNP"},{"id":"T31620","span":{"begin":710,"end":712},"obj":"IN"},{"id":"T31619","span":{"begin":699,"end":709},"obj":"VBN"},{"id":"T31618","span":{"begin":694,"end":698},"obj":"VBD"},{"id":"T31617","span":{"begin":687,"end":693},"obj":"NNS"},{"id":"T31616","span":{"begin":682,"end":686},"obj":"NNP"},{"id":"T31615","span":{"begin":677,"end":681},"obj":"NNP"},{"id":"T31614","span":{"begin":675,"end":676},"obj":"."},{"id":"T31613","span":{"begin":665,"end":675},"obj":"NNS"},{"id":"T31612","span":{"begin":660,"end":664},"obj":"JJ"},{"id":"T31611","span":{"begin":654,"end":659},"obj":"NN"},{"id":"T31610","span":{"begin":648,"end":653},"obj":"IN"},{"id":"T31609","span":{"begin":645,"end":647},"obj":"NN"},{"id":"T31608","span":{"begin":643,"end":644},"obj":"CD"},{"id":"T31607","span":{"begin":639,"end":642},"obj":"IN"},{"id":"T31606","span":{"begin":630,"end":638},"obj":"NNP"},{"id":"T31605","span":{"begin":625,"end":629},"obj":"IN"},{"id":"T31604","span":{"begin":614,"end":624},"obj":"VBN"},{"id":"T31603","span":{"begin":609,"end":613},"obj":"VBD"},{"id":"T31602","span":{"begin":607,"end":608},"obj":","},{"id":"T31601","span":{"begin":601,"end":607},"obj":"NNP"},{"id":"T31600","span":{"begin":593,"end":600},"obj":"NNP"},{"id":"T31599","span":{"begin":589,"end":592},"obj":"CC"},{"id":"T31598","span":{"begin":581,"end":588},"obj":"NNP"},{"id":"T31597","span":{"begin":576,"end":580},"obj":"IN"},{"id":"T31596","span":{"begin":567,"end":575},"obj":"VBN"},{"id":"T31595","span":{"begin":560,"end":566},"obj":"RB"},{"id":"T31594","span":{"begin":558,"end":559},"obj":","},{"id":"T31593","span":{"begin":553,"end":558},"obj":"NNS"},{"id":"T31592","span":{"begin":548,"end":552},"obj":"NNP"},{"id":"T31591","span":{"begin":546,"end":547},"obj":"-RRB-"},{"id":"T31590","span":{"begin":545,"end":546},"obj":"NNP"},{"id":"T31589","span":{"begin":544,"end":545},"obj":"-LRB-"},{"id":"T31665","span":{"begin":936,"end":943},"obj":"JJ"},{"id":"T31664","span":{"begin":934,"end":935},"obj":","},{"id":"T31663","span":{"begin":933,"end":934},"obj":"-RRB-"},{"id":"T31662","span":{"begin":932,"end":933},"obj":"NNP"},{"id":"T31661","span":{"begin":931,"end":932},"obj":"-LRB-"},{"id":"T31660","span":{"begin":928,"end":930},"obj":"NN"},{"id":"T31659","span":{"begin":925,"end":927},"obj":"CD"},{"id":"T31658","span":{"begin":922,"end":924},"obj":"IN"},{"id":"T31657","span":{"begin":916,"end":921},"obj":"NN"},{"id":"T31656","span":{"begin":906,"end":915},"obj":"NN"},{"id":"T31655","span":{"begin":903,"end":905},"obj":"IN"},{"id":"T31654","span":{"begin":894,"end":902},"obj":"VBN"},{"id":"T31653","span":{"begin":892,"end":893},"obj":","},{"id":"T31652","span":{"begin":882,"end":892},"obj":"NNS"},{"id":"T31651","span":{"begin":877,"end":881},"obj":"JJ"},{"id":"T31650","span":{"begin":871,"end":876},"obj":"NN"},{"id":"T31649","span":{"begin":865,"end":870},"obj":"IN"},{"id":"T31648","span":{"begin":856,"end":864},"obj":"NNP"},{"id":"T31647","span":{"begin":851,"end":855},"obj":"IN"},{"id":"T31646","span":{"begin":843,"end":850},"obj":"VBN"},{"id":"T31645","span":{"begin":838,"end":842},"obj":"VBD"},{"id":"T31644","span":{"begin":831,"end":837},"obj":"NNP"},{"id":"T31643","span":{"begin":827,"end":830},"obj":"CC"},{"id":"T31642","span":{"begin":820,"end":826},"obj":"NNP"},{"id":"T31641","span":{"begin":817,"end":819},"obj":"IN"},{"id":"T31640","span":{"begin":809,"end":816},"obj":"NNS"},{"id":"T31639","span":{"begin":805,"end":808},"obj":"NNP"},{"id":"T31638","span":{"begin":801,"end":804},"obj":"CC"},{"id":"T31637","span":{"begin":797,"end":800},"obj":"NNP"},{"id":"T31636","span":{"begin":793,"end":796},"obj":"CC"},{"id":"T31635","span":{"begin":785,"end":792},"obj":"NNP"},{"id":"T31634","span":{"begin":774,"end":784},"obj":"VBG"},{"id":"T31633","span":{"begin":768,"end":773},"obj":"NNS"},{"id":"T31632","span":{"begin":763,"end":767},"obj":"NNP"},{"id":"T31631","span":{"begin":761,"end":762},"obj":"-RRB-"},{"id":"T31630","span":{"begin":758,"end":761},"obj":"NNP"},{"id":"T31629","span":{"begin":757,"end":758},"obj":"-LRB-"}],"relations":[{"id":"R21777","pred":"punct","subj":"T31484","obj":"T31493"},{"id":"R21778","pred":"intj","subj":"T31485","obj":"T31493"},{"id":"R21779","pred":"punct","subj":"T31486","obj":"T31493"},{"id":"R21780","pred":"nmod","subj":"T31487","obj":"T31490"},{"id":"R21781","pred":"punct","subj":"T31488","obj":"T31487"},{"id":"R21782","pred":"compound","subj":"T31489","obj":"T31490"},{"id":"R21783","pred":"nsubjpass","subj":"T31490","obj":"T31493"},{"id":"R21784","pred":"auxpass","subj":"T31491","obj":"T31493"},{"id":"R21785","pred":"advmod","subj":"T31492","obj":"T31493"},{"id":"R21786","pred":"ROOT","subj":"T31493","obj":"T31493"},{"id":"R21787","pred":"prep","subj":"T31494","obj":"T31493"},{"id":"R21788","pred":"amod","subj":"T31495","obj":"T31497"},{"id":"R21789","pred":"compound","subj":"T31496","obj":"T31497"},{"id":"R21790","pred":"pobj","subj":"T31497","obj":"T31494"},{"id":"R21791","pred":"cc","subj":"T31498","obj":"T31497"},{"id":"R21792","pred":"conj","subj":"T31499","obj":"T31497"},{"id":"R21793","pred":"acl","subj":"T31500","obj":"T31499"},{"id":"R21794","pred":"dobj","subj":"T31501","obj":"T31500"},{"id":"R21795","pred":"cc","subj":"T31502","obj":"T31501"},{"id":"R21796","pred":"det","subj":"T31503","obj":"T31507"},{"id":"R21797","pred":"advmod","subj":"T31504","obj":"T31505"},{"id":"R21798","pred":"amod","subj":"T31505","obj":"T31507"},{"id":"R21799","pred":"compound","subj":"T31506","obj":"T31507"},{"id":"R21800","pred":"conj","subj":"T31507","obj":"T31501"},{"id":"R21801","pred":"punct","subj":"T31508","obj":"T31493"},{"id":"R21802","pred":"nsubjpass","subj":"T31509","obj":"T31511"},{"id":"R21803","pred":"auxpass","subj":"T31510","obj":"T31511"},{"id":"R21804","pred":"ROOT","subj":"T31511","obj":"T31511"},{"id":"R21805","pred":"agent","subj":"T31512","obj":"T31511"},{"id":"R21806","pred":"det","subj":"T31513","obj":"T31514"},{"id":"R21807","pred":"pobj","subj":"T31514","obj":"T31512"},{"id":"R21808","pred":"prep","subj":"T31515","obj":"T31514"},{"id":"R21809","pred":"pobj","subj":"T31516","obj":"T31515"},{"id":"R21810","pred":"prep","subj":"T31517","obj":"T31511"},{"id":"R21811","pred":"npadvmod","subj":"T31518","obj":"T31519"},{"id":"R21812","pred":"amod","subj":"T31519","obj":"T31520"},{"id":"R21813","pred":"pobj","subj":"T31520","obj":"T31517"},{"id":"R21814","pred":"punct","subj":"T31521","obj":"T31522"},{"id":"R21815","pred":"appos","subj":"T31522","obj":"T31520"},{"id":"R21816","pred":"punct","subj":"T31523","obj":"T31522"},{"id":"R21817","pred":"cc","subj":"T31524","obj":"T31522"},{"id":"R21818","pred":"nmod","subj":"T31525","obj":"T31529"},{"id":"R21819","pred":"cc","subj":"T31526","obj":"T31525"},{"id":"R21820","pred":"conj","subj":"T31527","obj":"T31525"},{"id":"R21821","pred":"amod","subj":"T31528","obj":"T31529"},{"id":"R21822","pred":"appos","subj":"T31529","obj":"T31520"},{"id":"R21823","pred":"prep","subj":"T31530","obj":"T31529"},{"id":"R21824","pred":"pobj","subj":"T31531","obj":"T31530"},{"id":"R21825","pred":"punct","subj":"T31532","obj":"T31533"},{"id":"R21826","pred":"appos","subj":"T31533","obj":"T31531"},{"id":"R21827","pred":"punct","subj":"T31534","obj":"T31531"},{"id":"R21828","pred":"punct","subj":"T31535","obj":"T31511"},{"id":"R21829","pred":"compound","subj":"T31536","obj":"T31537"},{"id":"R21830","pred":"nsubjpass","subj":"T31537","obj":"T31539"},{"id":"R21831","pred":"auxpass","subj":"T31538","obj":"T31539"},{"id":"R21832","pred":"ROOT","subj":"T31539","obj":"T31539"},{"id":"R21833","pred":"prep","subj":"T31540","obj":"T31539"},{"id":"R21834","pred":"nummod","subj":"T31541","obj":"T31542"},{"id":"R21835","pred":"pobj","subj":"T31542","obj":"T31540"},{"id":"R21836","pred":"punct","subj":"T31543","obj":"T31539"},{"id":"R21837","pred":"punct","subj":"T31544","obj":"T31547"},{"id":"R21838","pred":"nmod","subj":"T31545","obj":"T31547"},{"id":"R21839","pred":"punct","subj":"T31546","obj":"T31547"},{"id":"R21840","pred":"nmod","subj":"T31547","obj":"T31551"},{"id":"R21841","pred":"cc","subj":"T31548","obj":"T31547"},{"id":"R21842","pred":"compound","subj":"T31549","obj":"T31550"},{"id":"R21843","pred":"conj","subj":"T31550","obj":"T31547"},{"id":"R21844","pred":"nsubjpass","subj":"T31551","obj":"T31553"},{"id":"R21845","pred":"auxpass","subj":"T31552","obj":"T31553"},{"id":"R21846","pred":"ROOT","subj":"T31553","obj":"T31553"},{"id":"R21847","pred":"prep","subj":"T31554","obj":"T31553"},{"id":"R21848","pred":"pobj","subj":"T31555","obj":"T31554"},{"id":"R21849","pred":"cc","subj":"T31556","obj":"T31555"},{"id":"R21850","pred":"conj","subj":"T31557","obj":"T31555"},{"id":"R21851","pred":"prep","subj":"T31558","obj":"T31553"},{"id":"R21852","pred":"pobj","subj":"T31559","obj":"T31558"},{"id":"R21853","pred":"amod","subj":"T31560","obj":"T31561"},{"id":"R21854","pred":"pobj","subj":"T31561","obj":"T31558"},{"id":"R21855","pred":"prep","subj":"T31562","obj":"T31553"},{"id":"R21856","pred":"nummod","subj":"T31563","obj":"T31564"},{"id":"R21857","pred":"pobj","subj":"T31564","obj":"T31562"},{"id":"R21858","pred":"punct","subj":"T31565","obj":"T31553"},{"id":"R21859","pred":"advcl","subj":"T31566","obj":"T31553"},{"id":"R21860","pred":"agent","subj":"T31567","obj":"T31566"},{"id":"R21861","pred":"amod","subj":"T31568","obj":"T31569"},{"id":"R21862","pred":"pobj","subj":"T31569","obj":"T31567"},{"id":"R21863","pred":"advcl","subj":"T31570","obj":"T31566"},{"id":"R21864","pred":"det","subj":"T31571","obj":"T31573"},{"id":"R21865","pred":"amod","subj":"T31572","obj":"T31573"},{"id":"R21866","pred":"dobj","subj":"T31573","obj":"T31570"},{"id":"R21867","pred":"punct","subj":"T31574","obj":"T31553"},{"id":"R21868","pred":"compound","subj":"T31575","obj":"T31576"},{"id":"R21869","pred":"nsubjpass","subj":"T31576","obj":"T31587"},{"id":"R21870","pred":"punct","subj":"T31577","obj":"T31578"},{"id":"R21871","pred":"parataxis","subj":"T31578","obj":"T31576"},{"id":"R21872","pred":"punct","subj":"T31579","obj":"T31578"},{"id":"R21873","pred":"cc","subj":"T31580","obj":"T31576"},{"id":"R21874","pred":"conj","subj":"T31581","obj":"T31576"},{"id":"R21875","pred":"punct","subj":"T31582","obj":"T31583"},{"id":"R21876","pred":"appos","subj":"T31583","obj":"T31576"},{"id":"R21877","pred":"punct","subj":"T31584","obj":"T31583"},{"id":"R21878","pred":"nsubjpass","subj":"T31585","obj":"T31587"},{"id":"R21879","pred":"auxpass","subj":"T31586","obj":"T31587"},{"id":"R21880","pred":"ROOT","subj":"T31587","obj":"T31587"},{"id":"R21881","pred":"punct","subj":"T31588","obj":"T31587"},{"id":"R21882","pred":"punct","subj":"T31589","obj":"T31604"},{"id":"R21883","pred":"nmod","subj":"T31590","obj":"T31593"},{"id":"R21884","pred":"punct","subj":"T31591","obj":"T31590"},{"id":"R21885","pred":"compound","subj":"T31592","obj":"T31593"},{"id":"R21886","pred":"nsubjpass","subj":"T31593","obj":"T31604"},{"id":"R21887","pred":"punct","subj":"T31594","obj":"T31593"},{"id":"R21888","pred":"advmod","subj":"T31595","obj":"T31596"},{"id":"R21889","pred":"acl","subj":"T31596","obj":"T31593"},{"id":"R21890","pred":"prep","subj":"T31597","obj":"T31596"},{"id":"R21891","pred":"pobj","subj":"T31598","obj":"T31597"},{"id":"R21892","pred":"cc","subj":"T31599","obj":"T31598"},{"id":"R21893","pred":"compound","subj":"T31600","obj":"T31601"},{"id":"R21894","pred":"conj","subj":"T31601","obj":"T31598"},{"id":"R21895","pred":"punct","subj":"T31602","obj":"T31604"},{"id":"R21896","pred":"auxpass","subj":"T31603","obj":"T31604"},{"id":"R21897","pred":"ROOT","subj":"T31604","obj":"T31604"},{"id":"R21898","pred":"prep","subj":"T31605","obj":"T31604"},{"id":"R21899","pred":"pobj","subj":"T31606","obj":"T31605"},{"id":"R21900","pred":"prep","subj":"T31607","obj":"T31604"},{"id":"R21901","pred":"nummod","subj":"T31608","obj":"T31609"},{"id":"R21902","pred":"pobj","subj":"T31609","obj":"T31607"},{"id":"R21903","pred":"prep","subj":"T31610","obj":"T31609"},{"id":"R21904","pred":"npadvmod","subj":"T31611","obj":"T31612"},{"id":"R21905","pred":"amod","subj":"T31612","obj":"T31613"},{"id":"R21906","pred":"pobj","subj":"T31613","obj":"T31610"},{"id":"R21907","pred":"punct","subj":"T31614","obj":"T31604"},{"id":"R21908","pred":"compound","subj":"T31615","obj":"T31616"},{"id":"R21909","pred":"compound","subj":"T31616","obj":"T31617"},{"id":"R21910","pred":"nsubjpass","subj":"T31617","obj":"T31619"},{"id":"R21911","pred":"auxpass","subj":"T31618","obj":"T31619"},{"id":"R21912","pred":"ROOT","subj":"T31619","obj":"T31619"},{"id":"R21913","pred":"agent","subj":"T31620","obj":"T31619"},{"id":"R21914","pred":"pobj","subj":"T31621","obj":"T31620"},{"id":"R21915","pred":"cc","subj":"T31622","obj":"T31619"},{"id":"R21916","pred":"conj","subj":"T31623","obj":"T31619"},{"id":"R21917","pred":"advcl","subj":"T31624","obj":"T31623"},{"id":"R21918","pred":"nmod","subj":"T31625","obj":"T31627"},{"id":"R21919","pred":"nummod","subj":"T31626","obj":"T31627"},{"id":"R21920","pred":"dobj","subj":"T31627","obj":"T31624"},{"id":"R21921","pred":"punct","subj":"T31628","obj":"T31619"},{"id":"R21922","pred":"punct","subj":"T31629","obj":"T31646"},{"id":"R21923","pred":"compound","subj":"T31630","obj":"T31633"},{"id":"R21924","pred":"punct","subj":"T31631","obj":"T31632"},{"id":"R21925","pred":"compound","subj":"T31632","obj":"T31633"},{"id":"R21926","pred":"nsubjpass","subj":"T31633","obj":"T31646"},{"id":"R21927","pred":"acl","subj":"T31634","obj":"T31633"},{"id":"R21928","pred":"dobj","subj":"T31635","obj":"T31634"},{"id":"R21929","pred":"cc","subj":"T31636","obj":"T31635"},{"id":"R21930","pred":"conj","subj":"T31637","obj":"T31635"},{"id":"R21931","pred":"cc","subj":"T31638","obj":"T31637"},{"id":"R21932","pred":"conj","subj":"T31639","obj":"T31637"},{"id":"R21933","pred":"conj","subj":"T31640","obj":"T31635"},{"id":"R21934","pred":"prep","subj":"T31641","obj":"T31640"},{"id":"R21935","pred":"pobj","subj":"T31642","obj":"T31641"},{"id":"R21936","pred":"cc","subj":"T31643","obj":"T31642"},{"id":"R21937","pred":"conj","subj":"T31644","obj":"T31642"},{"id":"R21938","pred":"auxpass","subj":"T31645","obj":"T31646"},{"id":"R21939","pred":"ROOT","subj":"T31646","obj":"T31646"},{"id":"R21940","pred":"prep","subj":"T31647","obj":"T31646"},{"id":"R21941","pred":"pobj","subj":"T31648","obj":"T31647"},{"id":"R21942","pred":"prep","subj":"T31649","obj":"T31646"},{"id":"R21943","pred":"npadvmod","subj":"T31650","obj":"T31651"},{"id":"R21944","pred":"amod","subj":"T31651","obj":"T31652"},{"id":"R21945","pred":"pobj","subj":"T31652","obj":"T31649"},{"id":"R21946","pred":"punct","subj":"T31653","obj":"T31646"},{"id":"R21947","pred":"advcl","subj":"T31654","obj":"T31646"},{"id":"R21948","pred":"agent","subj":"T31655","obj":"T31654"},{"id":"R21949","pred":"compound","subj":"T31656","obj":"T31657"},{"id":"R21950","pred":"pobj","subj":"T31657","obj":"T31655"},{"id":"R21951","pred":"prep","subj":"T31658","obj":"T31654"},{"id":"R21952","pred":"nummod","subj":"T31659","obj":"T31660"},{"id":"R21953","pred":"pobj","subj":"T31660","obj":"T31658"},{"id":"R21954","pred":"punct","subj":"T31661","obj":"T31662"},{"id":"R21955","pred":"appos","subj":"T31662","obj":"T31660"},{"id":"R21956","pred":"punct","subj":"T31663","obj":"T31662"},{"id":"R21957","pred":"punct","subj":"T31664","obj":"T31660"},{"id":"R21958","pred":"amod","subj":"T31665","obj":"T31666"},{"id":"R21959","pred":"npadvmod","subj":"T31666","obj":"T31654"},{"id":"R21960","pred":"prep","subj":"T31667","obj":"T31654"},{"id":"R21961","pred":"nummod","subj":"T31668","obj":"T31669"},{"id":"R21962","pred":"pobj","subj":"T31669","obj":"T31667"},{"id":"R21963","pred":"punct","subj":"T31670","obj":"T31671"},{"id":"R21964","pred":"appos","subj":"T31671","obj":"T31669"},{"id":"R21965","pred":"punct","subj":"T31672","obj":"T31669"},{"id":"R21966","pred":"punct","subj":"T31673","obj":"T31669"},{"id":"R21967","pred":"cc","subj":"T31674","obj":"T31669"},{"id":"R21968","pred":"conj","subj":"T31675","obj":"T31669"},{"id":"R21969","pred":"prep","subj":"T31676","obj":"T31669"},{"id":"R21970","pred":"compound","subj":"T31677","obj":"T31678"},{"id":"R21971","pred":"compound","subj":"T31678","obj":"T31679"},{"id":"R21972","pred":"pobj","subj":"T31679","obj":"T31676"},{"id":"R21973","pred":"agent","subj":"T31680","obj":"T31654"},{"id":"R21974","pred":"pobj","subj":"T31681","obj":"T31680"},{"id":"R21975","pred":"prep","subj":"T31682","obj":"T31654"},{"id":"R21976","pred":"nummod","subj":"T31683","obj":"T31684"},{"id":"R21977","pred":"pobj","subj":"T31684","obj":"T31682"},{"id":"R21978","pred":"punct","subj":"T31685","obj":"T31686"},{"id":"R21979","pred":"appos","subj":"T31686","obj":"T31684"},{"id":"R21980","pred":"punct","subj":"T31687","obj":"T31684"},{"id":"R21981","pred":"punct","subj":"T31688","obj":"T31646"},{"id":"R21982","pred":"prep","subj":"T31689","obj":"T31697"},{"id":"R21983","pred":"det","subj":"T31690","obj":"T31691"},{"id":"R21984","pred":"pobj","subj":"T31691","obj":"T31689"},{"id":"R21985","pred":"punct","subj":"T31692","obj":"T31697"},{"id":"R21986","pred":"amod","subj":"T31693","obj":"T31694"},{"id":"R21987","pred":"compound","subj":"T31694","obj":"T31695"},{"id":"R21988","pred":"nsubjpass","subj":"T31695","obj":"T31697"},{"id":"R21989","pred":"auxpass","subj":"T31696","obj":"T31697"},{"id":"R21990","pred":"ROOT","subj":"T31697","obj":"T31697"},{"id":"R21991","pred":"punct","subj":"T31698","obj":"T31697"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
bionlp-st-ge-2016-test-tees
{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T31721","span":{"begin":774,"end":784},"obj":"Gene_expression"},{"id":"T31720","span":{"begin":979,"end":988},"obj":"Protein"},{"id":"T31719","span":{"begin":785,"end":792},"obj":"Protein"},{"id":"T31718","span":{"begin":742,"end":755},"obj":"Protein"},{"id":"T31717","span":{"begin":677,"end":686},"obj":"Protein"},{"id":"T31716","span":{"begin":630,"end":638},"obj":"Protein"},{"id":"T31715","span":{"begin":593,"end":607},"obj":"Protein"},{"id":"T31714","span":{"begin":581,"end":588},"obj":"Protein"},{"id":"T31713","span":{"begin":515,"end":518},"obj":"Protein"},{"id":"T31712","span":{"begin":511,"end":514},"obj":"Protein"},{"id":"T31711","span":{"begin":371,"end":379},"obj":"Protein"},{"id":"T31710","span":{"begin":337,"end":340},"obj":"Protein"},{"id":"T31709","span":{"begin":333,"end":336},"obj":"Protein"},{"id":"T31708","span":{"begin":325,"end":328},"obj":"Protein"},{"id":"T31707","span":{"begin":321,"end":324},"obj":"Protein"},{"id":"T31706","span":{"begin":123,"end":136},"obj":"Protein"},{"id":"T31705","span":{"begin":85,"end":92},"obj":"Protein"}],"relations":[{"id":"R21992","pred":"themeOf","subj":"T31719","obj":"T31721"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}
test3
{"project":"test3","denotations":[{"id":"T31176","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31175","span":{"begin":774,"end":784},"obj":"Gene_expression"},{"id":"T31174","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31173","span":{"begin":677,"end":681},"obj":"Protein"},{"id":"T31172","span":{"begin":154,"end":161},"obj":"Positive_regulation"},{"id":"T31171","span":{"begin":979,"end":983},"obj":"Protein"},{"id":"T31170","span":{"begin":748,"end":751},"obj":"Protein"},{"id":"T31169","span":{"begin":677,"end":681},"obj":"Protein"}],"text":"(A,B) L929 cells were stably infected with empty MSCV retrovirus or viruses encoding Myr-Akt or the catalytically inactive Myr-Akt K179M. Necroptosis was induced by the addition of zVAD.fmk under serum free conditions (A) or serum or serum free conditions with Nec-1 (B). Viability assays were performed after 24 hr. (C) Myr-Akt and Myr-Akt K179M cells were treated with zVAD.fmk and/or Nec-1 under serum free conditions for 9 hr, followed by western blot using the indicated antibodies. Endogenous Akt (∼) and Myr-Akt (*) bands are indicated. (D) L929 cells, stably infected with Myr-Akt and Myr-Akt K179KM, were stimulated with zVAD.fmk for 9 hr under serum free conditions. TNFα mRNA levels were determined by qRT-PCR and normalized using mouse 18S RNA. (E-G) L929 cells expressing Myr-Akt and Ala and Asp mutants of Thr308 and Ser473 were treated with zVAD.fmk under serum free conditions, followed by viability assay at 24 hr (E), western blot at 9 hr (F), or evaluation of TNFα mRNA levels by qRT-PCR at 9 hrs (G). In all graphs, average±SD was plotted."}