PMC:3583298 / 39883-41688 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3583298","sourcedb":"PMC","sourceid":"3583298","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3583298","text":"Figure 2 YAP promotes proliferation and survival in irradiated CGNPs\n(A) Western blot analysis of proliferation (cyclin D2) and apoptosis (cleaved caspase 3) in GFP- or YAP-transduced Shh-treated CGNPs untreated (NT) and 20 hours after γ-irradiation (IR) with 10 Gy. Panel to the right shows over-exposed image. Similar infection efficiencies were obtained with both retroviruses (Supplementary Figure 2).\n(B) Immunofluorescence analysis and quantification of cleaved caspase 3 and the proliferation markers Ki67 and phosphorylated histone H3 (P-Hist3) in GFP- or YAP-transduced CGNPs 20 hours post-irradiation.\n(C) Left, immunofluorescence analysis of BrdU incorporation (1-hour pulse) untreated (NT; top panels) and 18 hours after irradiation (IR; middle panels). The S-phase ratios for GFP and YAP-transduced CGNPs (%BrdU-positive of treated / %BrdU-positive untreated) are shown at the bottom. Irradiated GFP-transduced cells were normalized to non-irradiated GFP-transduced CGNPs; irradiated YAP-transduced CGNPs were normalized to non-irradiated YAP-transduced CGNPs. The higher S-phase ratio indicates that YAP-over-expressing CGNPs present a defect in the G1/S checkpoint. Right, immunofluorescence analysis of phosphorylated histone H3 before (NT; top panels) and 1 hour after radiation (IR; middle panels) in GFP- or YAP-transduced CGNPs. YAP-expressing CGNPs showed a higher mitotic ratio (%P-Hist3 positive of treated / % P-Hist3 positive untreated) (bottom right), indicating failure of the G2/M checkpoint in these cells. Again, irradiated GFP-transduced cells were normalized to non-irradiated GFP-transduced CGNPs; irradiated YAP-transduced CGNPs were normalized to non-irradiated YAP-transduced CGNPs.\nStatistically significant differences are indicated as (*) P \u003c 0.05; (***) P \u003c 0.001.","divisions":[{"label":"label","span":{"begin":0,"end":8}},{"label":"title","span":{"begin":10,"end":69}},{"label":"p","span":{"begin":70,"end":406}},{"label":"p","span":{"begin":407,"end":612}},{"label":"p","span":{"begin":613,"end":1719}}],"tracks":[]}