PMC:3583298 / 34193-34718 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3583298","sourcedb":"PMC","sourceid":"3583298","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3583298","text":"Total RNA from cerebellar granule neural precursors was extracted and purified using the MiRvana kit (Ambion). cDNA was prepared from 1 μg total RNA by using iScript cDNA Synthesis kit (Bio-Rad). qPCR was performed using TaqMan Universal PCR Master Mix (Applied Biosystems). RNA expression data were acquired and analysed using an StepOnePlus Real Time PCR system (Applied Biosystems). Average results and standard errors are presented. TaqMan probes used for qPCR were IGF2 (Mm01163433-m1) and control HPRT1 (Mm01545399-m1).","tracks":[]}