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{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3583298","sourcedb":"PMC","sourceid":"3583298","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3583298","text":"CGNP cultures were generated as previously described (Kenney and Rowitch 2000). Cells were plated on individual poly-DL-ornithine (Sigma) pre-coated plates or pre-coated glass coverslips. In all cases cells were treated with 3 μg/mL of Shh. Where indicated, LY 294002 (Sigma) was used at a concentration of 10 nM for 6h.","tracks":[{"project":"2_test","denotations":[{"id":"21874045-11074003-79138734","span":{"begin":73,"end":77},"obj":"11074003"},{"id":"21874045-11074003-79138734","span":{"begin":73,"end":77},"obj":"11074003"}],"attributes":[{"subj":"21874045-11074003-79138734","pred":"source","obj":"2_test"},{"subj":"21874045-11074003-79138734","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93adec","default":true}]}]}}