PMC:3583298 / 14484-15932 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3583298","sourcedb":"PMC","sourceid":"3583298","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3583298","text":"In contrast, at 9 hours after irradiation, YAP-infected cells had strikingly pronounced comet tails. Comet tails persisted in YAP-infected CGNPs 24 hours after irradiation, a time point at which we can detect proliferation in these cells (Figure 2). In order to analyze whether the DNA repair defect leads to chromosomal alterations, we performed metaphase spreads on GFP- or YAP-infected CGNPs 24 hours after irradiation. As shown in Figure 3C, YAP infection is associated with an increase in the number of cells with persistent DNA breaks. We also observed that YAP-transduced Pzp53med cells (Berman et al 2002) showed a similar loss of irradiation-induced foci and increased number of cells with DNA breaks after irradiation (Supplementary Figure 3A, B). These observations indicate that YAP expression enables cells to proliferate with unrepaired DNA, a condition which can lead to genomic instability, a hallmark of cancer cells. To address whether the effect of YAP was specific for radiation-induced DNA damage, we treated GFP- and YAP-infected cells with the antibiotic Bleomycin, which causes double strand breaks in DNA. Similar to what we observed in irradiated cells, 9 and 24 hours after treatment there were significantly fewer cells that contained foci among those over-expressing YAP, which indicates that the role of YAP promoting focus dis-assembly is not specific to radiation-induced damage in the DNA (Supplementary Figure 3C).","tracks":[{"project":"2_test","denotations":[{"id":"21874045-12202832-79138690","span":{"begin":608,"end":612},"obj":"12202832"},{"id":"21874045-12202832-79138690","span":{"begin":608,"end":612},"obj":"12202832"}],"attributes":[{"subj":"21874045-12202832-79138690","pred":"source","obj":"2_test"},{"subj":"21874045-12202832-79138690","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93cb","default":true}]}]}}