PMC:3570799 / 13630-14271 JSONTXT

{"project":"2_test","denotations":[{"id":"23450099-20211023-140447146","span":{"begin":56,"end":58},"obj":"20211023"},{"id":"23450099-20436475-140447147","span":{"begin":206,"end":208},"obj":"20436475"},{"id":"23450099-9023104-140447148","span":{"begin":583,"end":585},"obj":"9023104"},{"id":"23450099-17452365-140447149","span":{"begin":596,"end":598},"obj":"17452365"},{"id":"23450099-12520045-140447150","span":{"begin":607,"end":609},"obj":"12520045"},{"id":"23450099-11152613-140447151","span":{"begin":619,"end":621},"obj":"11152613"},{"id":"23450099-15223320-140447152","span":{"begin":637,"end":639},"obj":"15223320"}],"text":"Genome annotation\nGenes were identified using Prodigal [62] as part of the Oak Ridge National Laboratory genome annotation pipeline, followed by a round of manual curation using the JGI GenePRIMP pipeline [63]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) non-redundant database, UniProt, TIGRFam, Pfam, PRIAM, KEGG, COG, and InterPro databases. These data sources were combined to assert a product description for each predicted protein. Non-coding genes and miscellaneous features were predicted using tRNAscan-SE [64, RNAMMer [65], Rfam [66], TMHMM [67], and SignalP [68]."}