PMC:3514855 / 25104-26148 JSONTXT

{"project":"2_test","denotations":[{"id":"23069868-22193367-25970128","span":{"begin":169,"end":170},"obj":"22193367"},{"id":"23069868-16228363-25970129","span":{"begin":499,"end":500},"obj":"16228363"},{"id":"23069868-16228363-25970130","span":{"begin":944,"end":945},"obj":"16228363"}],"text":"‘PCC-M’ shows sequence differences in several genes compared with the reference sequence of ‘GT-Kazusa’ and also to the recently sequenced ‘GT-S’ strain. Kanesaki et al.9 concluded that 15 differences between the resequenced strains and the published GT-Kazusa sequence were annotation errors in the latter due to sequencing artefacts, a list to which we add two more putative errors in the database, differences #4 and #42 in Table 1. According to the proposed strain history in Ikeuchi and Tabata,8 the early division of Synechocystis sp. PCC 6803 into two branches occurred due to an insertion in spkA. Thus, our data suggest that the motile ‘PCC-M’ strain belongs to the motile PCC 6803 branch, whereas the non-motile ‘GT-Kazusa’, ‘GT-S’ and ‘GT-V’ strains are more closely related to each other and belong to the ATCC 27 184 branch. However, the 1-bp insertion in the pilC leading to ‘GT-Kazusa’ as described in the proposed strain history8 is not present in either ‘GT-S’ or ‘GT-V’, characterizing ‘GT-Kazusa’ as a more derived substrain."}