PMC:3514855 / 21453-22722
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3514855","sourcedb":"PMC","sourceid":"3514855","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3514855","text":"Two major mutations affect the clustered regularly interspaced short palindrome repeats-CRISPR-associated proteins (CRISPR-Cas) system, located on the plasmid pSYSA. CRISPR-Cas systems provide in many archaea and bacteria an adaptive immunity against invading DNA.38–44 The plasmid pSYSA encodes the three independent systems CRISPR1, CRISPR2 and CRISPR3. A 2399-bp deletion encompassing the spacer-repeat regions 15–47 of CRISPR1 was detected in ‘PCC-M’ (#43), which also eliminated the relatively short genes ssr7018, ssl7019, ssl7020 and ssl7021, annotated within the spacer-repeat array of CRISPR1. However, the theoretical protein sequences of these gene products show no conservation at all and might not constitute real genes. Nevertheless, the deletion of spacer-repeat regions 15–47 of CRISPR1 is severe, since compared with the reference, it has eliminated two-thirds, 33 of its 49 spacer-repeat units. The sequence analysis suggests that the recombination events leading to the deletion of spacer-repeat regions 15–47 must have occurred within the direct repeats. Thus, this recombination is in agreement with previous observations that the downstream ends of the repeat clusters are conserved such that deletions and recombination events occur internally.45","tracks":[{"project":"2_test","denotations":[{"id":"23069868-21294681-25970121","span":{"begin":264,"end":266},"obj":"21294681"},{"id":"23069868-20528693-25970121","span":{"begin":264,"end":266},"obj":"20528693"},{"id":"23069868-20056882-25970121","span":{"begin":264,"end":266},"obj":"20056882"},{"id":"23069868-20129051-25970121","span":{"begin":264,"end":266},"obj":"20129051"},{"id":"23069868-20072129-25970121","span":{"begin":264,"end":266},"obj":"20072129"},{"id":"23069868-21531607-25970121","span":{"begin":264,"end":266},"obj":"21531607"},{"id":"23069868-22337052-25970121","span":{"begin":264,"end":266},"obj":"22337052"},{"id":"23069868-19239620-25970122","span":{"begin":1267,"end":1269},"obj":"19239620"}],"attributes":[{"subj":"23069868-21294681-25970121","pred":"source","obj":"2_test"},{"subj":"23069868-20528693-25970121","pred":"source","obj":"2_test"},{"subj":"23069868-20056882-25970121","pred":"source","obj":"2_test"},{"subj":"23069868-20129051-25970121","pred":"source","obj":"2_test"},{"subj":"23069868-20072129-25970121","pred":"source","obj":"2_test"},{"subj":"23069868-21531607-25970121","pred":"source","obj":"2_test"},{"subj":"23069868-22337052-25970121","pred":"source","obj":"2_test"},{"subj":"23069868-19239620-25970122","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ecc093","default":true}]}]}}