PMC:3514855 / 14992-17275 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3514855","sourcedb":"PMC","sourceid":"3514855","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3514855","text":"3.2.1. SNPs unique to ‘PCC-M’\nSix of the 10 SNPs unique to ‘PCC-M’ are located within coding regions and cause amino acid substitutions or alter the length of the respective reading frame.\nA single basepair transversion in the gene sigF (slr1564; #39 in Table 1) is leading to a M231K substitution within the −35 element DNA-binding region29 of a group 3 sigma factor required for phototactic movement30 and salt-stress response.31 This SNP cannot lead to impaired motility as ‘PCC-M’ is motile but it might influence the DNA–protein interaction of SigF because positively charged residues such as lysine located in this part of the σ4.2 region can directly interact with DNA.29\nAnother transversion, in argB (slr1898; #8 in Table 1), leads to an S2N amino acid substitution in N-acetylglutamate kinase, the enzyme performing the first committed step of Arg biosynthesis. Transitions in sll1359 and slr1609 (#11 and #3 in Table 1) result in an N–K substitution at a very conserved position within a predicted cytochrome and an L608S (L548S) substitution in the long-chain acyl-CoA-synthetase Slr1609 that has been found crucial for fatty acid activation and the biosynthesis of alkanes.32 Interestingly, an unrelated SNP exists at position 488 923 within the slr1609 coding sequence in a strain ‘YF’, leading to a G546L (G486L) substitution.17 It should be noted that the slr1609 reading frame has been annotated 60 codons shorter (636 instead of 696 amino acids) during recent re-sequencing analyses,9,10 compared with the original annotation of ‘GT-Kazusa’ (numbers in brackets). The shorter Slr1609 protein of 636 amino acids is also consistent with the mapped start site of transcription at position 487 352,33 located 115 nt upstream of the revised start codon.\nA transition in slr0753 (#41 in Table 1) leads to a P113L substitution in a putative chloride efflux transport protein involved in maintaining the chloride ion concentration homoeostasis as required for a functional photosystem II.34\nA single basepair deletion in sll1496 (#38 in Table 1), encoding mannose-1-phosphate guanyltransferase, causes a frameshift and premature stop of the gene in ‘PCC-M’. The resulting protein is with 515 instead of 643 amino acids severely truncated and may be rendered function-less.","divisions":[{"label":"title","span":{"begin":8,"end":30}},{"label":"p","span":{"begin":31,"end":189}},{"label":"p","span":{"begin":190,"end":679}},{"label":"p","span":{"begin":680,"end":1767}},{"label":"p","span":{"begin":1768,"end":2001}}],"tracks":[{"project":"2_test","denotations":[{"id":"23069868-16903784-25970104","span":{"begin":340,"end":342},"obj":"16903784"},{"id":"23069868-11065366-25970105","span":{"begin":430,"end":432},"obj":"11065366"},{"id":"23069868-16903784-25970106","span":{"begin":677,"end":679},"obj":"16903784"},{"id":"23069868-22433663-25970107","span":{"begin":1187,"end":1189},"obj":"22433663"},{"id":"23069868-22375005-25970108","span":{"begin":1342,"end":1344},"obj":"22375005"},{"id":"23069868-22193367-25970109","span":{"begin":1502,"end":1503},"obj":"22193367"},{"id":"23069868-21803841-25970110","span":{"begin":1504,"end":1506},"obj":"21803841"},{"id":"23069868-21245330-25970111","span":{"begin":1713,"end":1715},"obj":"21245330"},{"id":"23069868-16621843-25970112","span":{"begin":1999,"end":2001},"obj":"16621843"}],"attributes":[{"subj":"23069868-16903784-25970104","pred":"source","obj":"2_test"},{"subj":"23069868-11065366-25970105","pred":"source","obj":"2_test"},{"subj":"23069868-16903784-25970106","pred":"source","obj":"2_test"},{"subj":"23069868-22433663-25970107","pred":"source","obj":"2_test"},{"subj":"23069868-22375005-25970108","pred":"source","obj":"2_test"},{"subj":"23069868-22193367-25970109","pred":"source","obj":"2_test"},{"subj":"23069868-21803841-25970110","pred":"source","obj":"2_test"},{"subj":"23069868-21245330-25970111","pred":"source","obj":"2_test"},{"subj":"23069868-16621843-25970112","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#bdec93","default":true}]}]}}