PMC:3492202 / 3741-4377 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3492202","sourcedb":"PMC","sourceid":"3492202","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3492202","text":"Considering the close geographical relationship of Chinese and Philippine bats, Reston-NP was used for the initial screening. ELISA plates were coated with the recombinant Reston-NP at approximately 100 ng/well and bat sera were tested in triplicates at a dilution of 1:100, followed by detection with horseradish peroxidase (HRP) conjugated Protein A/G (Pierce) at 1:20,000. Samples with a mean optical density 2.1-fold or higher than that of the negative control (OD450 value: 0.19) were considered positive. Positive serum samples were retested at dilutions of 1:100, 1:400, and 1:1600 against both Reston-NP and Zaire-NP (Figure 2).","tracks":[]}