PMC:3492202 / 2444-3317 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3492202","sourcedb":"PMC","sourceid":"3492202","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3492202","text":"The codon optimized nucleocapsid (NP) gene fragments (aa 410–653) of RESTV and EBOV were synthesized based on reference sequences downloaded from GenBank with reference numbers FJ621583 and L11365, respectively, and subcloned into pFastBac-HTb. The His-tagged truncated NP of RESTV (Reston-NP) or EBOV (Zaire-NP) were expressed in insect cells using the Bac-to-bac system (Invitrogen) and purified with a His.Bind Kit (Novagen) (Figure 1A \u0026 B) following the manufacturer’s instructions. Both the truncated Zaire-NP and Reston-NP strongly reacted to hyperimmune rabbit sera raised against the full-length NP protein of RESTV [10] by ELISA, although the optical density (OD450) readings to Reston-NP were higher than those to Zaire-NP under the same dilution (Figure 1C). The results indicated that both of these antigens were suitable for detection of ebolavirus antibodies.","tracks":[{"project":"2_test","denotations":[{"id":"23062147-21987755-143781705","span":{"begin":625,"end":627},"obj":"21987755"}],"attributes":[{"subj":"23062147-21987755-143781705","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#9f93ec","default":true}]}]}}