PMC:3475485 / 4438-5150 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3475485","sourcedb":"PMC","sourceid":"3475485","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3475485","text":"Establishment of MEF derived from wild-type and PKD2 mutation embryos\nMEF were acquired from PKD2 TG embryos or Pkd2 KO embryos during development (13.5 days) [11]. Heads and limes were removed from embryos. The remaining embryonic tissues were minced and dispersed in 0.05% trypsin prior to incubation at 37℃ for 15 min. Cells were plated in Dulbecco's Modified Eagle's Medium of Defined Minimal Essential medium (Welgene Biotech, Taipei, Taiwan) supplemented with 10% fetal bovine serum (Welgene Biotech) and were cultured at 37℃ in an atmosphere of 5% CO2 until confluent growth was achieved. MEF cells were frozen as stocks at the second passage and were used for the subsequent studies at the third passage.","divisions":[{"label":"Title","span":{"begin":0,"end":69}}],"tracks":[{"project":"2_test","denotations":[{"id":"23105924-19098310-44845729","span":{"begin":160,"end":162},"obj":"19098310"}],"attributes":[{"subj":"23105924-19098310-44845729","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#eca693","default":true}]}]}}