PMC:3475479 / 17131-17927 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3475479","sourcedb":"PMC","sourceid":"3475479","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3475479","text":"Conclusion\nNGS technology provides a cost-effective way of sequencing for novel whole-genome sequencing, resequencing, and expression profiling. Rohce/454 pyrosequencing is recommended to de novo assembly of whole prokaryote novel genomes, while hybrid assembly with Illumina/Solexa sequences would be optimal for whole eukaryote genomes and transcriptome studies of non-model organisms. Also, Illumina/Solexa sequencing is useful in detecting DNA variation, mapping the short-read resequence to the reference genome and profiling expressed genes in model organisms. Furthermore, the high-throughput NGS sequencing enables us to study with an in silico method in variable research application fields of molecular genetics, including population diversity and comparative genomics, in a short time.","divisions":[{"label":"Title","span":{"begin":0,"end":10}}],"tracks":[]}