PMC:3463543 / 5506-6066 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3463543","sourcedb":"PMC","sourceid":"3463543","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3463543","text":"Madin-Darby canine kidney (MDCK) cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 5% fetal calf serum (FCS). The stock of the IAV A/PR/8/34 (H1N1) was prepared with a plaque purified strain [22] in 10-day-old specific pathogen-free embryonated hens’ eggs. Virus stocks were stored as aliquots of allantoic fluid from infected embryos at −80°C until use. The titer of the virus stock was determined to be 1.78×108 median tissue culture infective dose (TCID50) mL−1. All virus titration experiments were performed in MDCK cells.","tracks":[{"project":"2_test","denotations":[{"id":"23056454-16945126-92972545","span":{"begin":225,"end":227},"obj":"16945126"}],"attributes":[{"subj":"23056454-16945126-92972545","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#a793ec","default":true}]}]}}