PMC:3463543 / 12190-13711 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3463543","sourcedb":"PMC","sourceid":"3463543","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3463543","text":"IAV Viability in Model Media v. RH\nAs shown in Figure 1A and 1B, IAV viabilities in four model media containing various combinations of salts and proteins were highest at extremely high (∼100%) and low (\u003c50%) RH and minimal at medium (50 to 84%) RH. At ∼100% RH, viabilities ranged from 8% to 57%, namely, 36±15% in PBS, 7.8±0.27% in DMEM, 11±4.1% in PBS+FCS, and 57±11% in DMEM+FCS of input virus. At ∼84% RH, viabilities were much lower (∼1–5%) but were of the same order of magnitude in all four media.\nFigure 1 Relationship between RH and IAV viability in (A) media with mainly salts, (B) media with salts plus proteins, and (C) mucus. Error bars denote standard deviations. At RH between 50% and 84%, the trends in viability diverged dramatically, depending on whether the media contained proteins or not. The viability in PBS decreased with decreasing RH and reached a minimum at 50% RH. In DMEM, the trend was similar, but the minimum occurred at an RH of 60% (Figure 1A). In contrast, in PBS+FCS, viabilities did not change significantly over this range of RH, and in DMEM+FCS, viabilities increased slightly with decreasing RH (Figure 1B).\nAt RH below 50%, viabilities were higher in all types of media compared to those at medium RH, although viability in PBS alone was significantly lower than in the three other media, possibly due to the lack of proteins.\nWe repeated the experiment to confirm these results. The percent viabilities recovered varied slightly, but trends in viability versus RH were similar.","divisions":[{"label":"title","span":{"begin":0,"end":34}},{"label":"p","span":{"begin":35,"end":505}},{"label":"figure","span":{"begin":506,"end":679}},{"label":"label","span":{"begin":506,"end":514}},{"label":"caption","span":{"begin":516,"end":679}},{"label":"title","span":{"begin":516,"end":679}},{"label":"p","span":{"begin":680,"end":1149}},{"label":"p","span":{"begin":1150,"end":1369}}],"tracks":[]}