PMC:3431878 / 6418-8001 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3431878","sourcedb":"PMC","sourceid":"3431878","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3431878","text":"The currently available genomic information on CA125 is based on two autonomous studies conducted in 2001 [17,18]. By screening a cDNA library generated using OVCAR-3 mRNA, Yin et al. identified a clone with a 5797 base pair (bp) nucleotide sequence [17] (reviewed in [19,20]). Although this region translated to a partial CA125 protein, it confirmed and revealed the existence of some key features. Importantly, they established the presence of partially conserved tandem repeats and were the first to describe the homology to the “sea urchin sperm protein, enterokinase, agrin” (SEA) domain. Yin et al. also postulated the existence of a transmembrane domain based upon a cluster of hydrophobic residues and a phosphorylation site based on a tyrosine phosphorylation consensus motif [17]. Furthermore, O-glycosylation was inferred due to the serine, threonine and proline content as well as N-glycosylation due to the asparagine content of the protein. These findings were supported and extended on by O’Brien and co-workers [18,21]. Here, a large transcript encoding CA125 was sequenced based upon a cyanogen bromide cleavage fragment of CA125 and a translated expressed sequence tag [16]. The resultant protein was described in three parts; an amino terminal domain, a repeat domain and a carboxy terminal domain (Table 1, Figure 1). The carboxy terminal domain reported by O’Brien et al. was homologous to the findings of Yin et al. [17,18,20]. One feature described only by O’Brien was the existence of a potential proteolytic cleavage site at amino acid residues 171–181 [18].","tracks":[{"project":"2_test","denotations":[{"id":"22949880-11369781-50810654","span":{"begin":107,"end":109},"obj":"11369781"},{"id":"22949880-11786729-50810655","span":{"begin":110,"end":112},"obj":"11786729"},{"id":"22949880-11369781-50810656","span":{"begin":251,"end":253},"obj":"11369781"},{"id":"22949880-15788735-50810657","span":{"begin":269,"end":271},"obj":"15788735"},{"id":"22949880-11786728-50810658","span":{"begin":272,"end":274},"obj":"11786728"},{"id":"22949880-11369781-50810659","span":{"begin":786,"end":788},"obj":"11369781"},{"id":"22949880-11786729-50810660","span":{"begin":1028,"end":1030},"obj":"11786729"},{"id":"22949880-12218296-50810661","span":{"begin":1031,"end":1033},"obj":"12218296"},{"id":"22949880-10228899-50810662","span":{"begin":1188,"end":1190},"obj":"10228899"},{"id":"22949880-11369781-50810663","span":{"begin":1439,"end":1441},"obj":"11369781"},{"id":"22949880-11786729-50810664","span":{"begin":1442,"end":1444},"obj":"11786729"},{"id":"22949880-11786728-50810665","span":{"begin":1445,"end":1447},"obj":"11786728"},{"id":"22949880-11786729-50810666","span":{"begin":1579,"end":1581},"obj":"11786729"},{"id":"T57307","span":{"begin":107,"end":109},"obj":"11369781"},{"id":"T17647","span":{"begin":110,"end":112},"obj":"11786729"},{"id":"T74833","span":{"begin":251,"end":253},"obj":"11369781"},{"id":"T21795","span":{"begin":269,"end":271},"obj":"15788735"},{"id":"T20828","span":{"begin":272,"end":274},"obj":"11786728"},{"id":"T91204","span":{"begin":786,"end":788},"obj":"11369781"},{"id":"T98513","span":{"begin":1028,"end":1030},"obj":"11786729"},{"id":"T75911","span":{"begin":1031,"end":1033},"obj":"12218296"},{"id":"T13278","span":{"begin":1188,"end":1190},"obj":"10228899"},{"id":"T89329","span":{"begin":1439,"end":1441},"obj":"11369781"},{"id":"T48694","span":{"begin":1442,"end":1444},"obj":"11786729"},{"id":"T2259","span":{"begin":1445,"end":1447},"obj":"11786728"},{"id":"T16302","span":{"begin":1579,"end":1581},"obj":"11786729"}],"attributes":[{"subj":"22949880-11369781-50810654","pred":"source","obj":"2_test"},{"subj":"22949880-11786729-50810655","pred":"source","obj":"2_test"},{"subj":"22949880-11369781-50810656","pred":"source","obj":"2_test"},{"subj":"22949880-15788735-50810657","pred":"source","obj":"2_test"},{"subj":"22949880-11786728-50810658","pred":"source","obj":"2_test"},{"subj":"22949880-11369781-50810659","pred":"source","obj":"2_test"},{"subj":"22949880-11786729-50810660","pred":"source","obj":"2_test"},{"subj":"22949880-12218296-50810661","pred":"source","obj":"2_test"},{"subj":"22949880-10228899-50810662","pred":"source","obj":"2_test"},{"subj":"22949880-11369781-50810663","pred":"source","obj":"2_test"},{"subj":"22949880-11786729-50810664","pred":"source","obj":"2_test"},{"subj":"22949880-11786728-50810665","pred":"source","obj":"2_test"},{"subj":"22949880-11786729-50810666","pred":"source","obj":"2_test"},{"subj":"T57307","pred":"source","obj":"2_test"},{"subj":"T17647","pred":"source","obj":"2_test"},{"subj":"T74833","pred":"source","obj":"2_test"},{"subj":"T21795","pred":"source","obj":"2_test"},{"subj":"T20828","pred":"source","obj":"2_test"},{"subj":"T91204","pred":"source","obj":"2_test"},{"subj":"T98513","pred":"source","obj":"2_test"},{"subj":"T75911","pred":"source","obj":"2_test"},{"subj":"T13278","pred":"source","obj":"2_test"},{"subj":"T89329","pred":"source","obj":"2_test"},{"subj":"T48694","pred":"source","obj":"2_test"},{"subj":"T2259","pred":"source","obj":"2_test"},{"subj":"T16302","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ecd2","default":true}]}]}}