PMC:3431878 / 20549-21974
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3431878","sourcedb":"PMC","sourceid":"3431878","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3431878","text":"Isolation of CA125 for the analysis of its molecular features represents a challenge for researchers, as routine high resolution methods such as SDS-PAGE are not ideal for very high molecular weight analytes. Typically, size exclusion chromatography (SEC) is used for CA125 purification; however the separation efficiency is limited and results in cross-contaminations [16]. Additional methods used for the prefractionation of CA125 include immuno-precipitation, affinity chromatography or a combination of these methods that are associated with similar disadvantages [12,16,28,48,50]. Subsequent identification of CA125 in the derived fractions is therefore necessary for reliable downstream analysis. However, CA125 identification solely based on antibody probing alone can lead to false-positive protein assignment as a result of antibody cross-reactivity [41]. Thus, characterization of these secondary proteins may lead to erroneous assignment of molecular attributes to CA125. Several studies show that mass spectrometry (MS) offers a suitable solution for independent CA125 identification (discussed below). It is, however, notable that the published protein sequence for CA125 is incomplete [17,18,21]. Although this may result in some peptide identifications being missed by search algorithms, mass spectrometric identification of CA125 is a viable approach that can complement antibody based identification methods.","tracks":[{"project":"2_test","denotations":[{"id":"22949880-10228899-50810738","span":{"begin":370,"end":372},"obj":"10228899"},{"id":"22949880-12615972-50810739","span":{"begin":569,"end":571},"obj":"12615972"},{"id":"22949880-10228899-50810740","span":{"begin":572,"end":574},"obj":"10228899"},{"id":"22949880-11125279-50810741","span":{"begin":575,"end":577},"obj":"11125279"},{"id":"22949880-18334732-50810742","span":{"begin":578,"end":580},"obj":"18334732"},{"id":"22949880-21901274-50810743","span":{"begin":581,"end":583},"obj":"21901274"},{"id":"22949880-11369781-50810744","span":{"begin":1200,"end":1202},"obj":"11369781"},{"id":"22949880-11786729-50810745","span":{"begin":1203,"end":1205},"obj":"11786729"},{"id":"22949880-12218296-50810746","span":{"begin":1206,"end":1208},"obj":"12218296"},{"id":"T46059","span":{"begin":370,"end":372},"obj":"10228899"},{"id":"T46141","span":{"begin":569,"end":571},"obj":"12615972"},{"id":"T35504","span":{"begin":572,"end":574},"obj":"10228899"},{"id":"T29950","span":{"begin":575,"end":577},"obj":"11125279"},{"id":"T91999","span":{"begin":578,"end":580},"obj":"18334732"},{"id":"T58996","span":{"begin":581,"end":583},"obj":"21901274"},{"id":"T29033","span":{"begin":1200,"end":1202},"obj":"11369781"},{"id":"T39769","span":{"begin":1203,"end":1205},"obj":"11786729"},{"id":"T53745","span":{"begin":1206,"end":1208},"obj":"12218296"}],"attributes":[{"subj":"22949880-10228899-50810738","pred":"source","obj":"2_test"},{"subj":"22949880-12615972-50810739","pred":"source","obj":"2_test"},{"subj":"22949880-10228899-50810740","pred":"source","obj":"2_test"},{"subj":"22949880-11125279-50810741","pred":"source","obj":"2_test"},{"subj":"22949880-18334732-50810742","pred":"source","obj":"2_test"},{"subj":"22949880-21901274-50810743","pred":"source","obj":"2_test"},{"subj":"22949880-11369781-50810744","pred":"source","obj":"2_test"},{"subj":"22949880-11786729-50810745","pred":"source","obj":"2_test"},{"subj":"22949880-12218296-50810746","pred":"source","obj":"2_test"},{"subj":"T46059","pred":"source","obj":"2_test"},{"subj":"T46141","pred":"source","obj":"2_test"},{"subj":"T35504","pred":"source","obj":"2_test"},{"subj":"T29950","pred":"source","obj":"2_test"},{"subj":"T91999","pred":"source","obj":"2_test"},{"subj":"T58996","pred":"source","obj":"2_test"},{"subj":"T29033","pred":"source","obj":"2_test"},{"subj":"T39769","pred":"source","obj":"2_test"},{"subj":"T53745","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93dfec","default":true}]}]}}