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PMC:340383 / 21150-23115 JSONTXT

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Id Subject Object Predicate Lexical cue
T5370 592-602 NN denotes atmosphere
T5369 579-580 DT denotes a
T5368 576-578 IN denotes in
T5367 573-575 NN denotes °C
T5366 571-573 CD denotes 37
T5365 568-570 IN denotes at
T5364 552-556 VBD denotes were
T5363 557-567 VBN denotes maintained
T5362 544-551 NNS denotes neurons
T5361 540-543 DT denotes The
T5360 539-615 sentence denotes The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2.
T5359 538-539 . denotes .
T5358 533-538 RB denotes below
T5357 523-532 VBN denotes described
T5356 520-522 IN denotes as
T5355 511-519 VBN denotes obtained
T5354 491-499 JJ denotes cortical
T5353 500-510 NNS denotes astrocytes
T5352 485-490 NN denotes mouse
T5351 482-484 IN denotes of
T5350 462-471 JJ denotes confluent
T5349 472-481 NN denotes monolayer
T5348 460-461 DT denotes a
T5347 455-459 IN denotes with
T5346 448-454 VBN denotes coated
T5345 437-447 NNS denotes coverslips
T5344 431-436 NN denotes glass
T5343 428-430 IN denotes on
T5342 416-420 VBD denotes were
T5341 421-427 VBN denotes plated
T5340 396-407 JJ denotes hippocampal
T5339 384-395 JJ denotes dissociated
T5338 408-415 NNS denotes neurons
T5337 380-383 DT denotes The
T5336 379-539 sentence denotes The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below.
T5335 378-379 . denotes .
T5334 377-378 -RRB- denotes )
T5333 360-364 NNP denotes Life
T5332 365-377 NNP denotes Technologies
T5331 359-360 -LRB- denotes (
T5330 351-358 NN denotes trypsin
T5329 349-350 NN denotes %
T5328 345-349 CD denotes 0.25
T5327 340-344 CC denotes plus
T5326 338-339 -RRB- denotes )
T5325 321-325 NNP denotes Life
T5324 326-338 NNP denotes Technologies
T5323 320-321 -LRB- denotes (
T5322 312-319 NN denotes culture
T5321 301-311 NN denotes suspension
T5320 297-300 IN denotes for
T5319 288-296 VBN denotes modified
T5318 286-287 -RRB- denotes )
T5317 270-279 JJ denotes essential
T5316 280-286 NN denotes medium
T5315 262-269 JJ denotes minimum
T5314 261-262 -LRB- denotes (
T5313 257-260 NN denotes MEM
T5312 254-256 IN denotes in
T5311 251-253 NN denotes °C
T5310 249-251 CD denotes 37
T5309 246-248 IN denotes at
T5308 242-245 NN denotes min
T5307 239-241 CD denotes 20
T5306 235-238 IN denotes for
T5305 220-224 RB denotes then
T5304 215-219 VBD denotes were
T5303 225-234 VBN denotes incubated
T5302 207-214 NNS denotes tissues
T5301 203-206 DT denotes The
T5300 202-379 sentence denotes The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies).
T5299 201-202 . denotes .
T5298 196-201 FW denotes utero
T5297 193-195 FW denotes in
T5296 188-192 NNS denotes days
T5295 185-187 CD denotes 16
T5294 182-184 IN denotes at
T5293 174-181 NNS denotes embryos
T5292 168-173 NN denotes mouse
T5291 163-167 IN denotes from
T5290 159-162 RP denotes out
T5289 144-148 VBD denotes were
T5288 130-143 NNS denotes hippocampuses
T5287 126-129 DT denotes the
T5286 124-126 , denotes ,
T5285 119-124 NN denotes brief
T5284 149-158 VBN denotes dissected
T5283 116-118 IN denotes In
T5282 115-202 sentence denotes In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero.
T5281 114-115 . denotes .
T5280 113-114 -RRB- denotes ]
T5279 112-113 CD denotes 6
T5278 111-112 -LRB- denotes [
T5277 91-101 RB denotes previously
T5276 102-110 VBN denotes reported
T5275 88-90 IN denotes as
T5274 74-78 VBD denotes were
T5273 79-87 VBN denotes prepared
T5272 58-64 NN denotes neuron
T5271 65-73 NNS denotes cultures
T5270 46-57 JJ denotes Hippocampal
T5269 45-115 sentence denotes Hippocampal neuron cultures were prepared as previously reported [6].
T5268 30-45 NN denotes immunostanining
T5267 26-29 CC denotes and
T5266 9-13 NN denotes cell
T5265 14-25 NN denotes preparation
T5264 0-8 JJ denotes Neuronal
T5655 1964-1965 . denotes .
T5654 1963-1964 -RRB- denotes )
T5653 1961-1963 NNP denotes MD
T5652 1959-1961 , denotes ,
T5651 1950-1959 NNP denotes Frederick
T5650 1948-1950 , denotes ,
T5649 1931-1936 NNP denotes Scion
T5648 1937-1948 NNP denotes Corporation
T5647 1930-1931 -LRB- denotes (
T5646 1915-1920 NN denotes Image
T5645 1909-1914 NNP denotes Scion
T5644 1921-1929 NN denotes software
T5643 1905-1908 DT denotes the
T5642 1902-1904 IN denotes by
T5641 1893-1901 VBN denotes acquired
T5640 1889-1892 CC denotes and
T5639 1878-1881 NN denotes CCD
T5638 1882-1888 NN denotes camera
T5637 1876-1877 DT denotes a
T5636 1871-1875 IN denotes with
T5635 1857-1861 VBD denotes were
T5634 1862-1870 VBN denotes captured
T5633 1850-1856 NNS denotes Images
T5632 1849-1965 sentence denotes Images were captured with a CCD camera and acquired by the Scion Image software (Scion Corporation, Frederick, MD).
T5631 1848-1849 . denotes .
T5630 1847-1848 -RRB- denotes )
T5629 1837-1841 NNP denotes Carl
T5628 1842-1847 NNP denotes Zeiss
T5627 1836-1837 -LRB- denotes (
T5560 1498-1500 DT denotes an
T5559 1493-1497 IN denotes with
T5558 1478-1482 VBD denotes were
T5557 1472-1477 NNS denotes cells
T5556 1468-1471 DT denotes the
T5555 1466-1468 , denotes ,
T5554 1454-1457 NN denotes PBS
T5553 1458-1466 NN denotes solution
T5552 1443-1447 NN denotes goat
T5551 1448-1453 NN denotes serum
T5550 1441-1442 NN denotes %
T5549 1440-1441 CD denotes 1
T5548 1435-1439 IN denotes with
T5547 1427-1434 NN denotes washing
T5546 1417-1426 JJ denotes extensive
T5545 1483-1492 VBN denotes incubated
T5544 1411-1416 IN denotes After
T5543 1410-1626 sentence denotes After extensive washing with 1% goat serum PBS solution, the cells were incubated with an Alex 488 conjugated secondary antibody (1:100 in 1% goat serum PBS solution, Molecular Probes) for 3 hrs at room temperature.
T5542 1409-1410 . denotes .
T5541 1407-1409 NN denotes °C
T5540 1406-1407 CD denotes 4
T5539 1403-1405 IN denotes at
T5538 1393-1402 RB denotes overnight
T5537 1391-1392 -RRB- denotes )
T5536 1387-1391 CD denotes 3000
T5535 1386-1387 SYM denotes :
T5534 1385-1386 CD denotes 1
T5533 1384-1385 -LRB- denotes (
T5532 1365-1374 JJ denotes anti-ACDP
T5531 1354-1364 JJ denotes polyclonal
T5530 1347-1353 NN denotes rabbit
T5529 1375-1383 NN denotes antibody
T5528 1345-1346 DT denotes a
T5527 1340-1344 IN denotes with
T5526 1330-1339 VBN denotes incubated
T5525 1317-1329 RB denotes subsequently
T5524 1313-1316 CC denotes and
T5523 1311-1313 , denotes ,
T5522 1301-1305 NN denotes goat
T5521 1294-1300 JJ denotes normal
T5520 1291-1293 CD denotes 30
T5519 1290-1291 SYM denotes :
T5518 1306-1311 NN denotes serum
T5517 1289-1290 CD denotes 1
T5516 1278-1288 VBG denotes containing
T5515 1260-1268 VBG denotes blocking
T5514 1269-1277 NN denotes solution
T5513 1258-1259 DT denotes a
T5512 1253-1257 IN denotes with
T5511 1238-1242 VBD denotes were
T5510 1232-1237 NNS denotes cells
T5509 1228-1231 DT denotes the
T5508 1226-1228 , denotes ,
T5507 1221-1226 NNS denotes times
T5506 1215-1220 CD denotes three
T5505 1211-1214 NN denotes PBS
T5504 1206-1210 IN denotes with
T5503 1198-1205 VBG denotes washing
T5502 1243-1252 VBN denotes incubated
T5501 1192-1197 IN denotes After
T5500 1191-1410 sentence denotes After washing with PBS three times, the cells were incubated with a blocking solution containing 1:30 normal goat serum, and subsequently incubated with a rabbit polyclonal anti-ACDP antibody (1:3000) overnight at 4°C.
T5499 1190-1191 . denotes .
T5498 1188-1190 NN denotes hr
T5497 1186-1187 CD denotes 1
T5496 1182-1185 IN denotes for
T5495 1179-1181 NN denotes °C
T5494 1178-1179 CD denotes 4
T5493 1175-1177 IN denotes at
T5492 1170-1171 HYPH denotes -
T5491 1169-1170 NN denotes X
T5490 1162-1168 NN denotes Triton
T5489 1171-1174 NN denotes 100
T5488 1160-1161 NN denotes %
T5487 1157-1160 CD denotes 0.4
T5486 1153-1156 CC denotes and
T5485 1149-1152 NN denotes PFA
T5484 1147-1148 NN denotes %
T5483 1146-1147 CD denotes 4
T5482 1135-1145 VBG denotes containing
T5481 1126-1134 NN denotes solution
T5480 1124-1125 DT denotes a
T5479 1121-1123 IN denotes in
T5478 1111-1120 VBN denotes incubated
T5477 1106-1110 RB denotes then
T5476 1102-1105 CC denotes and
T5475 1099-1101 NN denotes °C
T5474 1098-1099 CD denotes 4
T5473 1095-1097 IN denotes at
T5472 1091-1094 NNS denotes hrs
T5471 1088-1090 CD denotes 12
T5470 1084-1087 IN denotes for
T5469 1082-1083 -RRB- denotes )
T5468 1079-1082 NN denotes PFA
T5467 1078-1079 -LRB- denotes (
T5466 1061-1077 NN denotes paraformaldehyde
T5465 1059-1060 NN denotes %
T5464 1058-1059 CD denotes 4
T5463 1047-1057 VBG denotes containing
T5462 1043-1046 NN denotes PBS
T5461 1040-1042 IN denotes in
T5460 1028-1033 RB denotes first
T5459 1023-1027 VBD denotes were
T5458 1012-1022 NNS denotes coverslips
T5457 1008-1011 DT denotes the
T5456 1005-1007 IN denotes on
T5455 999-1004 NNS denotes cells
T5454 990-998 JJ denotes neuronal
T5453 988-990 , denotes ,
T5452 974-988 NN denotes immunostaining
T5451 1034-1039 VBN denotes fixed
T5450 970-973 IN denotes For
T5449 969-1191 sentence denotes For immunostaining, neuronal cells on the coverslips were first fixed in PBS containing 4% paraformaldehyde (PFA) for 12 hrs at 4°C and then incubated in a solution containing 4% PFA and 0.4% Triton X-100 at 4°C for 1 hr.
T5448 968-969 . denotes .
T5447 958-968 NNS denotes coverslips
T5446 950-957 VBG denotes coating
T5445 946-949 IN denotes for
T5444 936-940 VBD denotes were
T5443 930-935 NNS denotes cells
T5442 926-929 DT denotes the
T5441 924-926 , denotes ,
T5440 918-924 NN denotes debris
T5439 909-917 JJ denotes cellular
T5438 904-908 IN denotes with
T5437 898-903 NNS denotes media
T5436 894-897 DT denotes the
T5435 891-893 IN denotes of
T5434 884-890 NN denotes remove
T5433 941-945 VBN denotes used
T5432 878-883 IN denotes After
T5431 877-969 sentence denotes After remove of the media with cellular debris, the cells were used for coating coverslips.
T5430 876-877 . denotes .
T5429 874-876 NN denotes °C
T5428 872-874 CD denotes 37
T5427 869-871 IN denotes at
T5426 861-862 SYM denotes –
T5425 862-864 CD denotes 24
T5424 859-861 CD denotes 12
T5423 865-868 NNS denotes hrs
T5422 848-858 JJ denotes additional
T5421 844-847 IN denotes for
T5420 835-843 VBN denotes cultured
T5419 831-834 CC denotes and
T5418 829-830 -RRB- denotes )
T5417 824-829 NNP denotes Sigma
T5416 823-824 -LRB- denotes (
T5415 802-810 NN denotes cytosine
T5414 811-822 NN denotes arabinoside
T5413 800-801 NN denotes M
T5412 797-798 SYM denotes -
T5411 798-799 CD denotes 5
T5410 795-797 CD denotes 10
T5409 792-794 IN denotes to
T5408 779-783 VBD denotes were
T5407 784-791 VBN denotes exposed
T5406 773-778 NNS denotes cells
T5405 769-772 DT denotes The
T5404 768-877 sentence denotes The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C.
T5403 767-768 . denotes .
T5402 758-767 JJ denotes confluent
T5401 752-757 IN denotes until
T5400 748-751 NN denotes CO2
T5399 746-747 NN denotes %
T5398 745-746 CD denotes 5
T5397 740-744 IN denotes with
T5396 718-728 JJ denotes humidified
T5395 729-739 NN denotes atmosphere
T5394 716-717 DT denotes a
T5393 713-715 IN denotes in
T5392 710-712 NN denotes °C
T5391 708-710 CD denotes 37
T5390 705-707 IN denotes at
T5389 698-704 NNS denotes flasks
T5388 690-697 NN denotes culture
T5387 687-689 IN denotes in
T5386 676-680 VBD denotes were
T5385 667-675 NNS denotes cortices
T5384 661-666 NN denotes mouse
T5383 653-660 JJ denotes newborn
T5382 648-652 IN denotes from
T5381 636-647 VBN denotes dissociated
T5380 681-686 VBN denotes grown
T5379 625-635 NNS denotes astrocytes
T5378 616-624 JJ denotes Cortical
T5377 615-768 sentence denotes Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent.
T5376 614-615 . denotes .
T5375 611-614 NN denotes CO2
T5374 609-610 NN denotes %
T5373 608-609 CD denotes 5
T5372 603-607 IN denotes with
T5371 581-591 JJ denotes humidified
T5626 1816-1824 JJ denotes confocal
T5625 1825-1835 NN denotes microscope
T5624 1814-1815 DT denotes a
T5623 1808-1813 IN denotes under
T5622 1796-1800 VBD denotes were
T5621 1801-1807 VBN denotes viewed
T5620 1790-1795 NNS denotes cells
T5619 1786-1789 DT denotes The
T5618 1785-1849 sentence denotes The cells were viewed under a confocal microscope (Carl Zeiss).
T5617 1784-1785 . denotes .
T5616 1761-1777 JJ denotes anti-photobleach
T5615 1754-1755 HYPH denotes -
T5614 1755-1760 VBN denotes based
T5613 1746-1754 NN denotes glycerol
T5612 1778-1784 NN denotes medium
T5611 1744-1745 DT denotes a
T5610 1739-1743 IN denotes with
T5609 1730-1731 HYPH denotes -
T5608 1725-1730 NN denotes cover
T5607 1720-1724 VBD denotes were
T5606 1709-1719 NNS denotes coverslips
T5605 1705-1708 DT denotes the
T5604 1702-1704 IN denotes on
T5603 1687-1695 JJ denotes neuronal
T5602 1696-1701 NNS denotes cells
T5601 1683-1686 DT denotes the
T5600 1681-1683 , denotes ,
T5599 1669-1672 NN denotes PBS
T5598 1673-1681 NN denotes solution
T5597 1658-1662 NN denotes goat
T5596 1663-1668 NN denotes serum
T5595 1656-1657 NN denotes %
T5594 1655-1656 CD denotes 1
T5593 1650-1654 IN denotes with
T5592 1643-1649 NNS denotes washes
T5591 1637-1642 JJ denotes final
T5590 1731-1738 VBN denotes slipped
T5589 1627-1636 IN denotes Following
T5588 1626-1785 sentence denotes Following final washes with 1% goat serum PBS solution, the neuronal cells on the coverslips were cover-slipped with a glycerol-based anti-photobleach medium.
T5587 1625-1626 . denotes .
T5586 1614-1625 NN denotes temperature
T5585 1609-1613 NN denotes room
T5584 1606-1608 IN denotes at
T5583 1602-1605 NNS denotes hrs
T5582 1600-1601 CD denotes 3
T5581 1596-1599 IN denotes for
T5580 1594-1595 -RRB- denotes )
T5579 1578-1587 NNP denotes Molecular
T5578 1576-1578 , denotes ,
T5577 1564-1567 NN denotes PBS
T5576 1568-1576 NN denotes solution
T5575 1553-1557 NN denotes goat
T5574 1558-1563 NN denotes serum
T5573 1551-1552 NN denotes %
T5572 1550-1551 CD denotes 1
T5571 1547-1549 IN denotes in
T5570 1543-1546 CD denotes 100
T5569 1542-1543 SYM denotes :
T5568 1541-1542 CD denotes 1
T5567 1588-1594 NNP denotes Probes
T5566 1540-1541 -LRB- denotes (
T5565 1521-1530 JJ denotes secondary
T5564 1510-1520 JJ denotes conjugated
T5563 1506-1509 CD denotes 488
T5562 1501-1505 NNP denotes Alex
T5561 1531-1539 NN denotes antibody
R3141 T5264 T5265 amod Neuronal,preparation
R3142 T5266 T5265 compound cell,preparation
R3143 T5267 T5265 cc and,preparation
R3144 T5268 T5265 conj immunostanining,preparation
R3145 T5270 T5271 amod Hippocampal,cultures
R3146 T5271 T5273 nsubjpass cultures,prepared
R3147 T5272 T5271 compound neuron,cultures
R3148 T5274 T5273 auxpass were,prepared
R3149 T5275 T5276 mark as,reported
R3150 T5276 T5273 advcl reported,prepared
R3151 T5277 T5276 advmod previously,reported
R3152 T5278 T5279 punct [,6
R3153 T5279 T5273 parataxis 6,prepared
R3154 T5280 T5279 punct ],6
R3155 T5281 T5273 punct .,prepared
R3156 T5283 T5284 prep In,dissected
R3157 T5285 T5283 pobj brief,In
R3158 T5286 T5284 punct ", ",dissected
R3159 T5287 T5288 det the,hippocampuses
R3160 T5288 T5284 nsubjpass hippocampuses,dissected
R3161 T5289 T5284 auxpass were,dissected
R3162 T5290 T5284 prt out,dissected
R3163 T5291 T5284 prep from,dissected
R3164 T5292 T5293 compound mouse,embryos
R3165 T5293 T5291 pobj embryos,from
R3166 T5294 T5284 prep at,dissected
R3167 T5295 T5296 nummod 16,days
R3168 T5296 T5294 pobj days,at
R3169 T5297 T5298 advmod in,utero
R3170 T5298 T5296 advmod utero,days
R3171 T5299 T5284 punct .,dissected
R3172 T5301 T5302 det The,tissues
R3173 T5302 T5303 nsubjpass tissues,incubated
R3174 T5304 T5303 auxpass were,incubated
R3175 T5305 T5303 advmod then,incubated
R3176 T5306 T5303 prep for,incubated
R3177 T5307 T5308 nummod 20,min
R3178 T5308 T5306 pobj min,for
R3179 T5309 T5303 prep at,incubated
R3180 T5310 T5311 nummod 37,°C
R3181 T5311 T5309 pobj °C,at
R3182 T5312 T5303 prep in,incubated
R3183 T5313 T5312 pobj MEM,in
R3184 T5314 T5313 punct (,MEM
R3185 T5315 T5316 amod minimum,medium
R3186 T5316 T5313 appos medium,MEM
R3187 T5317 T5316 amod essential,medium
R3188 T5318 T5313 punct ),MEM
R3189 T5319 T5313 acl modified,MEM
R3190 T5320 T5319 prep for,modified
R3191 T5321 T5322 compound suspension,culture
R3192 T5322 T5320 pobj culture,for
R3193 T5323 T5324 punct (,Technologies
R3194 T5324 T5313 parataxis Technologies,MEM
R3195 T5325 T5324 compound Life,Technologies
R3196 T5326 T5324 punct ),Technologies
R3197 T5327 T5313 cc plus,MEM
R3198 T5328 T5329 nummod 0.25,%
R3199 T5329 T5330 compound %,trypsin
R3200 T5330 T5313 conj trypsin,MEM
R3201 T5331 T5332 punct (,Technologies
R3202 T5332 T5330 parataxis Technologies,trypsin
R3203 T5333 T5332 compound Life,Technologies
R3204 T5334 T5332 punct ),Technologies
R3205 T5335 T5303 punct .,incubated
R3206 T5337 T5338 det The,neurons
R3207 T5338 T5341 nsubjpass neurons,plated
R3208 T5339 T5338 amod dissociated,neurons
R3209 T5340 T5338 amod hippocampal,neurons
R3210 T5342 T5341 auxpass were,plated
R3211 T5343 T5341 prep on,plated
R3212 T5344 T5345 compound glass,coverslips
R3213 T5345 T5343 pobj coverslips,on
R3214 T5346 T5345 acl coated,coverslips
R3215 T5347 T5346 prep with,coated
R3216 T5348 T5349 det a,monolayer
R3217 T5349 T5347 pobj monolayer,with
R3218 T5350 T5349 amod confluent,monolayer
R3219 T5351 T5349 prep of,monolayer
R3220 T5352 T5353 nmod mouse,astrocytes
R3221 T5353 T5351 pobj astrocytes,of
R3222 T5354 T5353 amod cortical,astrocytes
R3223 T5355 T5353 acl obtained,astrocytes
R3224 T5356 T5357 mark as,described
R3225 T5357 T5355 advcl described,obtained
R3226 T5358 T5357 advmod below,described
R3227 T5359 T5341 punct .,plated
R3228 T5361 T5362 det The,neurons
R3229 T5362 T5363 nsubjpass neurons,maintained
R3230 T5364 T5363 auxpass were,maintained
R3231 T5365 T5363 prep at,maintained
R3232 T5366 T5367 nummod 37,°C
R3233 T5367 T5365 pobj °C,at
R3234 T5368 T5363 prep in,maintained
R3235 T5369 T5370 det a,atmosphere
R3236 T5370 T5368 pobj atmosphere,in
R3237 T5371 T5370 amod humidified,atmosphere
R3238 T5372 T5370 prep with,atmosphere
R3239 T5373 T5374 nummod 5,%
R3240 T5374 T5375 compound %,CO2
R3241 T5375 T5372 pobj CO2,with
R3242 T5376 T5363 punct .,maintained
R3243 T5378 T5379 amod Cortical,astrocytes
R3244 T5379 T5380 nsubjpass astrocytes,grown
R3245 T5381 T5379 acl dissociated,astrocytes
R3246 T5382 T5381 prep from,dissociated
R3247 T5383 T5384 amod newborn,mouse
R3248 T5384 T5385 compound mouse,cortices
R3249 T5385 T5382 pobj cortices,from
R3250 T5386 T5380 auxpass were,grown
R3251 T5387 T5380 prep in,grown
R3252 T5388 T5389 compound culture,flasks
R3253 T5389 T5387 pobj flasks,in
R3254 T5390 T5380 prep at,grown
R3255 T5391 T5392 nummod 37,°C
R3256 T5392 T5390 pobj °C,at
R3257 T5393 T5380 prep in,grown
R3258 T5394 T5395 det a,atmosphere
R3259 T5395 T5393 pobj atmosphere,in
R3260 T5396 T5395 amod humidified,atmosphere
R3261 T5397 T5395 prep with,atmosphere
R3262 T5398 T5399 nummod 5,%
R3263 T5399 T5400 compound %,CO2
R3264 T5400 T5397 pobj CO2,with
R3265 T5401 T5380 prep until,grown
R3266 T5402 T5401 pcomp confluent,until
R3267 T5403 T5380 punct .,grown
R3268 T5405 T5406 det The,cells
R3269 T5406 T5407 nsubjpass cells,exposed
R3270 T5408 T5407 auxpass were,exposed
R3271 T5409 T5407 prep to,exposed
R3272 T5410 T5411 quantmod 10,5
R3273 T5411 T5413 nummod 5,M
R3274 T5412 T5411 punct -,5
R3275 T5413 T5414 compound M,arabinoside
R3276 T5414 T5409 pobj arabinoside,to
R3277 T5415 T5414 compound cytosine,arabinoside
R3278 T5416 T5417 punct (,Sigma
R3279 T5417 T5414 parataxis Sigma,arabinoside
R3280 T5418 T5417 punct ),Sigma
R3281 T5419 T5407 cc and,exposed
R3282 T5420 T5407 conj cultured,exposed
R3283 T5421 T5420 prep for,cultured
R3284 T5422 T5423 amod additional,hrs
R3285 T5423 T5421 pobj hrs,for
R3286 T5424 T5425 quantmod 12,24
R3287 T5425 T5423 nummod 24,hrs
R3288 T5426 T5425 punct –,24
R3289 T5427 T5420 prep at,cultured
R3290 T5428 T5429 nummod 37,°C
R3291 T5429 T5427 pobj °C,at
R3292 T5430 T5407 punct .,exposed
R3293 T5432 T5433 prep After,used
R3294 T5434 T5432 pobj remove,After
R3295 T5435 T5434 prep of,remove
R3296 T5436 T5437 det the,media
R3297 T5437 T5435 pobj media,of
R3298 T5438 T5434 prep with,remove
R3299 T5439 T5440 amod cellular,debris
R3300 T5440 T5438 pobj debris,with
R3301 T5441 T5433 punct ", ",used
R3302 T5442 T5443 det the,cells
R3303 T5443 T5433 nsubjpass cells,used
R3304 T5444 T5433 auxpass were,used
R3305 T5445 T5433 prep for,used
R3306 T5446 T5445 pcomp coating,for
R3307 T5447 T5446 dobj coverslips,coating
R3308 T5448 T5433 punct .,used
R3309 T5450 T5451 prep For,fixed
R3310 T5452 T5450 pobj immunostaining,For
R3311 T5453 T5451 punct ", ",fixed
R3312 T5454 T5455 amod neuronal,cells
R3313 T5455 T5451 nsubjpass cells,fixed
R3314 T5456 T5455 prep on,cells
R3315 T5457 T5458 det the,coverslips
R3316 T5458 T5456 pobj coverslips,on
R3317 T5459 T5451 auxpass were,fixed
R3318 T5460 T5451 advmod first,fixed
R3319 T5461 T5451 prep in,fixed
R3320 T5462 T5461 pobj PBS,in
R3321 T5463 T5462 acl containing,PBS
R3322 T5464 T5465 nummod 4,%
R3323 T5465 T5466 compound %,paraformaldehyde
R3324 T5466 T5463 dobj paraformaldehyde,containing
R3325 T5467 T5466 punct (,paraformaldehyde
R3326 T5468 T5466 appos PFA,paraformaldehyde
R3327 T5469 T5463 punct ),containing
R3328 T5470 T5463 prep for,containing
R3329 T5471 T5472 nummod 12,hrs
R3330 T5472 T5470 pobj hrs,for
R3331 T5473 T5463 prep at,containing
R3332 T5474 T5475 nummod 4,°C
R3333 T5475 T5473 pobj °C,at
R3334 T5476 T5451 cc and,fixed
R3335 T5477 T5478 advmod then,incubated
R3336 T5478 T5451 conj incubated,fixed
R3337 T5479 T5478 prep in,incubated
R3338 T5480 T5481 det a,solution
R3339 T5481 T5479 pobj solution,in
R3340 T5482 T5481 acl containing,solution
R3341 T5483 T5484 nummod 4,%
R3342 T5484 T5485 compound %,PFA
R3343 T5485 T5482 dobj PFA,containing
R3344 T5486 T5485 cc and,PFA
R3345 T5487 T5488 nummod 0.4,%
R3346 T5488 T5489 compound %,100
R3347 T5489 T5485 conj 100,PFA
R3348 T5490 T5489 compound Triton,100
R3349 T5491 T5489 compound X,100
R3350 T5492 T5489 punct -,100
R3351 T5493 T5478 prep at,incubated
R3352 T5494 T5495 nummod 4,°C
R3353 T5495 T5493 pobj °C,at
R3354 T5496 T5478 prep for,incubated
R3355 T5497 T5498 nummod 1,hr
R3356 T5498 T5496 pobj hr,for
R3357 T5499 T5451 punct .,fixed
R3358 T5501 T5502 prep After,incubated
R3359 T5503 T5501 pcomp washing,After
R3360 T5504 T5503 prep with,washing
R3361 T5505 T5504 pobj PBS,with
R3362 T5506 T5507 nummod three,times
R3363 T5507 T5503 npadvmod times,washing
R3364 T5508 T5502 punct ", ",incubated
R3365 T5509 T5510 det the,cells
R3366 T5510 T5502 nsubjpass cells,incubated
R3367 T5511 T5502 auxpass were,incubated
R3368 T5512 T5502 prep with,incubated
R3369 T5513 T5514 det a,solution
R3370 T5514 T5512 pobj solution,with
R3371 T5515 T5514 amod blocking,solution
R3372 T5516 T5514 acl containing,solution
R3373 T5517 T5518 nummod 1,serum
R3374 T5518 T5516 dobj serum,containing
R3375 T5519 T5520 punct :,30
R3376 T5520 T5517 prep 30,1
R3377 T5521 T5518 amod normal,serum
R3378 T5522 T5518 compound goat,serum
R3379 T5523 T5502 punct ", ",incubated
R3380 T5524 T5502 cc and,incubated
R3381 T5525 T5526 advmod subsequently,incubated
R3382 T5526 T5502 conj incubated,incubated
R3383 T5527 T5526 prep with,incubated
R3384 T5528 T5529 det a,antibody
R3385 T5529 T5527 pobj antibody,with
R3386 T5530 T5529 nmod rabbit,antibody
R3387 T5531 T5529 amod polyclonal,antibody
R3388 T5532 T5529 amod anti-ACDP,antibody
R3389 T5533 T5534 punct (,1
R3390 T5534 T5529 parataxis 1,antibody
R3391 T5535 T5536 punct :,3000
R3392 T5536 T5534 prep 3000,1
R3393 T5537 T5534 punct ),1
R3394 T5538 T5526 advmod overnight,incubated
R3395 T5539 T5526 prep at,incubated
R3396 T5540 T5541 nummod 4,°C
R3397 T5541 T5539 pobj °C,at
R3398 T5542 T5502 punct .,incubated
R3399 T5544 T5545 prep After,incubated
R3400 T5546 T5547 amod extensive,washing
R3401 T5547 T5544 pobj washing,After
R3402 T5548 T5547 prep with,washing
R3403 T5549 T5550 nummod 1,%
R3404 T5550 T5551 compound %,serum
R3405 T5551 T5553 compound serum,solution
R3406 T5552 T5551 compound goat,serum
R3407 T5553 T5548 pobj solution,with
R3408 T5554 T5553 compound PBS,solution
R3409 T5555 T5545 punct ", ",incubated
R3410 T5556 T5557 det the,cells
R3411 T5557 T5545 nsubjpass cells,incubated
R3412 T5558 T5545 auxpass were,incubated
R3413 T5559 T5545 prep with,incubated
R3414 T5560 T5561 det an,antibody
R3415 T5561 T5559 pobj antibody,with
R3416 T5562 T5561 nmod Alex,antibody
R3417 T5563 T5562 nummod 488,Alex
R3418 T5564 T5561 amod conjugated,antibody
R3419 T5565 T5561 amod secondary,antibody
R3420 T5566 T5567 punct (,Probes
R3421 T5567 T5561 parataxis Probes,antibody
R3422 T5568 T5567 dep 1,Probes
R3423 T5569 T5570 punct :,100
R3424 T5570 T5568 prep 100,1
R3425 T5571 T5568 prep in,1
R3426 T5572 T5573 nummod 1,%
R3427 T5573 T5574 compound %,serum
R3428 T5574 T5576 compound serum,solution
R3429 T5575 T5574 compound goat,serum
R3430 T5576 T5571 pobj solution,in
R3431 T5577 T5576 compound PBS,solution
R3432 T5578 T5567 punct ", ",Probes
R3433 T5579 T5567 compound Molecular,Probes
R3434 T5580 T5567 punct ),Probes
R3435 T5581 T5545 prep for,incubated
R3436 T5582 T5583 nummod 3,hrs
R3437 T5583 T5581 pobj hrs,for
R3438 T5584 T5545 prep at,incubated
R3439 T5585 T5586 compound room,temperature
R3440 T5586 T5584 pobj temperature,at
R3441 T5587 T5545 punct .,incubated
R3442 T5589 T5590 prep Following,slipped
R3443 T5591 T5592 amod final,washes
R3444 T5592 T5589 pobj washes,Following
R3445 T5593 T5592 prep with,washes
R3446 T5594 T5595 nummod 1,%
R3447 T5595 T5596 compound %,serum
R3448 T5596 T5598 compound serum,solution
R3449 T5597 T5596 compound goat,serum
R3450 T5598 T5593 pobj solution,with
R3451 T5599 T5598 compound PBS,solution
R3452 T5600 T5590 punct ", ",slipped
R3453 T5601 T5602 det the,cells
R3454 T5602 T5590 nsubjpass cells,slipped
R3455 T5603 T5602 amod neuronal,cells
R3456 T5604 T5602 prep on,cells
R3457 T5605 T5606 det the,coverslips
R3458 T5606 T5604 pobj coverslips,on
R3459 T5607 T5590 auxpass were,slipped
R3460 T5608 T5590 dep cover,slipped
R3461 T5609 T5590 punct -,slipped
R3462 T5610 T5590 prep with,slipped
R3463 T5611 T5612 det a,medium
R3464 T5612 T5610 pobj medium,with
R3465 T5613 T5614 npadvmod glycerol,based
R3466 T5614 T5612 amod based,medium
R3467 T5615 T5614 punct -,based
R3468 T5616 T5612 amod anti-photobleach,medium
R3469 T5617 T5590 punct .,slipped
R3470 T5619 T5620 det The,cells
R3471 T5620 T5621 nsubjpass cells,viewed
R3472 T5622 T5621 auxpass were,viewed
R3473 T5623 T5621 prep under,viewed
R3474 T5624 T5625 det a,microscope
R3475 T5625 T5623 pobj microscope,under
R3476 T5626 T5625 amod confocal,microscope
R3477 T5627 T5628 punct (,Zeiss
R3478 T5628 T5625 parataxis Zeiss,microscope
R3479 T5629 T5628 compound Carl,Zeiss
R3480 T5630 T5628 punct ),Zeiss
R3481 T5631 T5621 punct .,viewed
R3482 T5633 T5634 nsubjpass Images,captured
R3483 T5635 T5634 auxpass were,captured
R3484 T5636 T5634 prep with,captured
R3485 T5637 T5638 det a,camera
R3486 T5638 T5636 pobj camera,with
R3487 T5639 T5638 compound CCD,camera
R3488 T5640 T5634 cc and,captured
R3489 T5641 T5634 conj acquired,captured
R3490 T5642 T5641 prep by,acquired
R3491 T5643 T5644 det the,software
R3492 T5644 T5642 pobj software,by
R3493 T5645 T5644 compound Scion,software
R3494 T5646 T5644 compound Image,software
R3495 T5647 T5648 punct (,Corporation
R3496 T5648 T5644 parataxis Corporation,software
R3497 T5649 T5648 compound Scion,Corporation
R3498 T5650 T5648 punct ", ",Corporation
R3499 T5651 T5648 npadvmod Frederick,Corporation
R3500 T5652 T5648 punct ", ",Corporation
R3501 T5653 T5648 npadvmod MD,Corporation
R3502 T5654 T5648 punct ),Corporation
R3503 T5655 T5634 punct .,captured

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5207 1746-1754 CHEBI:17754 denotes glycerol
T5206 1687-1701 CL:0000540 denotes neuronal cells
T5205 1673-1681 CHEBI:75958 denotes solution
T5204 1658-1662 NCBITaxon:9925 denotes goat
T5203 1568-1576 CHEBI:75958 denotes solution
T5202 1553-1557 NCBITaxon:9925 denotes goat
T5201 1531-1539 GO:0042571 denotes antibody
T5200 1510-1520 MOP:0000779 denotes conjugated
T5199 1501-1509 CHEBI:52661 denotes Alex 488
T5198 1458-1466 CHEBI:75958 denotes solution
T5197 1443-1447 NCBITaxon:9925 denotes goat
T5196 1375-1383 GO:0042571 denotes antibody
T5195 1347-1353 NCBITaxon:9986 denotes rabbit
T5194 1301-1305 NCBITaxon:9925 denotes goat
T5193 1269-1277 CHEBI:75958 denotes solution
T5192 1162-1174 CHEBI:9750 denotes Triton X-100
T5191 1126-1134 CHEBI:75958 denotes solution
T5190 990-1004 CL:0000540 denotes neuronal cells
T5189 802-822 CHEBI:28680 denotes cytosine arabinoside
T5188 748-751 CHEBI:16526 denotes CO2
T5187 681-686 GO:0040007 denotes grown
T5186 667-675 UBERON:0001851 denotes cortices
T5185 661-666 NCBITaxon:10088 denotes mouse
T5184 616-635 CL:0002605 denotes Cortical astrocytes
T5183 616-624 UBERON:0001851 denotes Cortical
T5182 611-614 CHEBI:16526 denotes CO2
T5181 544-551 CL:0000540 denotes neurons
T5180 491-510 CL:0002605 denotes cortical astrocytes
T5179 491-499 UBERON:0001851 denotes cortical
T5178 485-490 NCBITaxon:10088 denotes mouse
T5177 396-415 CL:0002608 denotes hippocampal neurons
T5176 351-358 PR:000027795 denotes trypsin
T5175 207-214 UBERON:0000479 denotes tissues
T5174 174-181 UBERON:0000922 denotes embryos
T5173 168-173 NCBITaxon:10088 denotes mouse
T5172 46-64 CL:0002608 denotes Hippocampal neuron
T5171 0-13 CL:0000540 denotes Neuronal cell

2_test

Id Subject Object Predicate Lexical cue
14723793-8657283-10642200 112-113 8657283 denotes 6

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5263 1790-1795 CL_GO_EXT:cell denotes cells
T5262 1746-1754 CHEBI:17754 denotes glycerol
T5261 1696-1701 CL_GO_EXT:cell denotes cells
T5260 1687-1701 CL:0000540 denotes neuronal cells
T5259 1673-1681 CHEBI:75958 denotes solution
T5258 1658-1662 NCBITaxon:9925 denotes goat
T5257 1588-1594 CHEBI_SO_EXT:molecular_probe denotes Probes
T5256 1578-1587 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T5255 1568-1576 CHEBI:75958 denotes solution
T5254 1553-1557 NCBITaxon:9925 denotes goat
T5253 1531-1539 GO:0042571 denotes antibody
T5252 1510-1520 MOP:0000779 denotes conjugated
T5251 1501-1509 CHEBI:52661 denotes Alex 488
T5250 1472-1477 CL_GO_EXT:cell denotes cells
T5249 1458-1466 CHEBI:75958 denotes solution
T5248 1443-1447 NCBITaxon:9925 denotes goat
T5247 1375-1383 GO:0042571 denotes antibody
T5246 1370-1374 PR_EXT:ACDP_or_CNNM denotes ACDP
T5245 1347-1353 NCBITaxon:9986 denotes rabbit
T5244 1301-1305 NCBITaxon:9925 denotes goat
T5243 1269-1277 CHEBI:75958 denotes solution
T5242 1232-1237 CL_GO_EXT:cell denotes cells
T5241 1162-1174 CHEBI:9750 denotes Triton X-100
T5240 1149-1152 CHEBI_EXT:paraformaldehye denotes PFA
T5239 1126-1134 CHEBI:75958 denotes solution
T5238 1079-1082 CHEBI_EXT:paraformaldehye denotes PFA
T5237 1061-1077 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T5236 999-1004 CL_GO_EXT:cell denotes cells
T5235 990-1004 CL:0000540 denotes neuronal cells
T5234 930-935 CL_GO_EXT:cell denotes cells
T5233 909-917 CL_GO_EXT:cell denotes cellular
T5232 802-822 CHEBI:28680 denotes cytosine arabinoside
T5231 773-778 CL_GO_EXT:cell denotes cells
T5230 748-751 CHEBI:16526 denotes CO2
T5229 681-686 GO:0040007 denotes grown
T5228 667-675 UBERON:0001851 denotes cortices
T5227 661-666 NCBITaxon:10088 denotes mouse
T5226 653-660 PATO_UBERON_EXT:neonate_or_newborn denotes newborn
T5225 616-635 CL:0002605 denotes Cortical astrocytes
T5224 616-624 UBERON:0001851 denotes Cortical
T5223 611-614 CHEBI:16526 denotes CO2
T5222 544-551 CL:0000540 denotes neurons
T5221 491-510 CL:0002605 denotes cortical astrocytes
T5220 491-499 UBERON:0001851 denotes cortical
T5219 485-490 NCBITaxon:10088 denotes mouse
T5218 396-415 CL:0002608 denotes hippocampal neurons
T5217 396-407 UBERON_EXT:hippocampus_proper_or_hippocampal_formation denotes hippocampal
T5216 351-358 PR_EXT:000027795 denotes trypsin
T5215 207-214 UBERON:0000479 denotes tissues
T5214 174-181 UBERON:0000922 denotes embryos
T5213 168-173 NCBITaxon:10088 denotes mouse
T5212 130-143 UBERON_EXT:hippocampus_proper_or_hippocampal_formation denotes hippocampuses
T5211 46-64 CL:0002608 denotes Hippocampal neuron
T5210 46-57 UBERON_EXT:hippocampus_proper_or_hippocampal_formation denotes Hippocampal
T5209 9-13 CL_GO_EXT:cell denotes cell
T5208 0-13 CL:0000540 denotes Neuronal cell