Id |
Subject |
Object |
Predicate |
Lexical cue |
T4786 |
630-631 |
. |
denotes |
. |
T4785 |
629-630 |
-RRB- |
denotes |
] |
T4784 |
627-628 |
, |
denotes |
, |
T4783 |
626-627 |
CD |
denotes |
3 |
T4782 |
628-629 |
CD |
denotes |
4 |
T4781 |
625-626 |
-LRB- |
denotes |
[ |
T4780 |
618-624 |
RBR |
denotes |
longer |
T4779 |
615-617 |
CC |
denotes |
or |
T4778 |
605-614 |
NN |
denotes |
overnight |
T4777 |
601-604 |
IN |
denotes |
for |
T4776 |
595-600 |
NNS |
denotes |
films |
T4775 |
589-594 |
NN |
denotes |
X-ray |
T4774 |
586-588 |
IN |
denotes |
to |
T4773 |
573-577 |
VBD |
denotes |
were |
T4772 |
578-585 |
VBN |
denotes |
exposed |
T4771 |
565-572 |
NNS |
denotes |
filters |
T4770 |
558-564 |
JJ |
denotes |
Washed |
T4769 |
557-631 |
sentence |
denotes |
Washed filters were exposed to X-ray films for overnight or longer [3,4]. |
T4768 |
556-557 |
. |
denotes |
. |
T4767 |
552-553 |
HYPH |
denotes |
- |
T4766 |
553-556 |
NN |
denotes |
min |
T4765 |
550-552 |
CD |
denotes |
15 |
T4764 |
546-549 |
IN |
denotes |
for |
T4763 |
543-545 |
NN |
denotes |
°C |
T4762 |
541-543 |
CD |
denotes |
65 |
T4761 |
538-540 |
IN |
denotes |
at |
T4760 |
536-537 |
-RRB- |
denotes |
) |
T4759 |
531-532 |
NN |
denotes |
% |
T4758 |
528-531 |
CD |
denotes |
0.1 |
T4757 |
526-528 |
, |
denotes |
, |
T4756 |
522-523 |
SYM |
denotes |
× |
T4755 |
523-526 |
NN |
denotes |
SSC |
T4754 |
518-522 |
CD |
denotes |
0.25 |
T4753 |
533-536 |
NN |
denotes |
SDS |
T4752 |
517-518 |
-LRB- |
denotes |
( |
T4751 |
514-516 |
CD |
denotes |
II |
T4750 |
507-513 |
NN |
denotes |
buffer |
T4749 |
499-506 |
NN |
denotes |
washing |
T4748 |
494-498 |
IN |
denotes |
with |
T4747 |
488-493 |
RB |
denotes |
twice |
T4746 |
481-487 |
VBD |
denotes |
washed |
T4745 |
476-480 |
RB |
denotes |
then |
T4744 |
472-475 |
CC |
denotes |
and |
T4743 |
470-472 |
, |
denotes |
, |
T4742 |
466-467 |
HYPH |
denotes |
- |
T4741 |
467-470 |
NN |
denotes |
min |
T4740 |
464-466 |
CD |
denotes |
15 |
T4739 |
460-463 |
IN |
denotes |
for |
T4738 |
457-459 |
NN |
denotes |
°C |
T4737 |
455-457 |
CD |
denotes |
42 |
T4736 |
452-454 |
IN |
denotes |
at |
T4735 |
450-451 |
-RRB- |
denotes |
) |
T4734 |
445-446 |
NN |
denotes |
% |
T4733 |
442-445 |
CD |
denotes |
0.1 |
T4732 |
440-442 |
, |
denotes |
, |
T4731 |
436-437 |
SYM |
denotes |
× |
T4730 |
437-440 |
NN |
denotes |
SSC |
T4729 |
435-436 |
CD |
denotes |
2 |
T4728 |
447-450 |
NN |
denotes |
SDS |
T4727 |
434-435 |
-LRB- |
denotes |
( |
T4726 |
432-433 |
CD |
denotes |
I |
T4725 |
425-431 |
NN |
denotes |
buffer |
T4724 |
417-424 |
VBG |
denotes |
washing |
T4723 |
412-416 |
IN |
denotes |
with |
T4722 |
406-411 |
RB |
denotes |
twice |
T4721 |
394-398 |
VBD |
denotes |
were |
T4720 |
399-405 |
VBN |
denotes |
washed |
T4719 |
386-393 |
NNS |
denotes |
filters |
T4718 |
382-385 |
DT |
denotes |
The |
T4717 |
381-557 |
sentence |
denotes |
The filters were washed twice with washing buffer I (2×SSC, 0.1% SDS) at 42°C for 15-min, and then washed twice with washing buffer II (0.25×SSC, 0.1% SDS) at 65°C for 15-min. |
T4716 |
380-381 |
. |
denotes |
. |
T4715 |
371-380 |
RB |
denotes |
overnight |
T4714 |
367-370 |
RP |
denotes |
out |
T4713 |
354-358 |
VBD |
denotes |
were |
T4712 |
359-366 |
VBN |
denotes |
carried |
T4711 |
339-353 |
NNS |
denotes |
Hybridizations |
T4710 |
338-381 |
sentence |
denotes |
Hybridizations were carried out overnight. |
T4709 |
337-338 |
. |
denotes |
. |
T4708 |
336-337 |
-RRB- |
denotes |
) |
T4707 |
319-323 |
NNP |
denotes |
Life |
T4706 |
324-336 |
NNP |
denotes |
Technologies |
T4705 |
318-319 |
-LRB- |
denotes |
( |
T4704 |
301-310 |
NN |
denotes |
extension |
T4703 |
294-300 |
NN |
denotes |
primer |
T4702 |
287-293 |
JJ |
denotes |
random |
T4701 |
311-317 |
NN |
denotes |
system |
T4700 |
283-286 |
DT |
denotes |
the |
T4699 |
277-282 |
VBG |
denotes |
using |
T4698 |
272-276 |
NN |
denotes |
dCTP |
T4697 |
267-268 |
HYPH |
denotes |
- |
T4696 |
268-271 |
NN |
denotes |
32P |
T4695 |
266-267 |
NN |
denotes |
α |
T4694 |
261-265 |
IN |
denotes |
with |
T4693 |
253-260 |
VBN |
denotes |
labeled |
T4692 |
233-245 |
JJ |
denotes |
untranslated |
T4691 |
231-232 |
SYM |
denotes |
' |
T4690 |
246-252 |
NN |
denotes |
region |
T4689 |
230-231 |
CD |
denotes |
3 |
T4688 |
226-229 |
DT |
denotes |
the |
T4687 |
222-225 |
CC |
denotes |
and |
T4686 |
217-221 |
NN |
denotes |
exon |
T4685 |
212-216 |
JJ |
denotes |
last |
T4684 |
207-211 |
IN |
denotes |
from |
T4683 |
205-206 |
-RRB- |
denotes |
) |
T4682 |
199-202 |
CD |
denotes |
350 |
T4681 |
192-198 |
IN |
denotes |
around |
T4680 |
203-205 |
NN |
denotes |
bp |
T4679 |
191-192 |
-LRB- |
denotes |
( |
T4678 |
178-181 |
NN |
denotes |
PCR |
T4677 |
182-190 |
NN |
denotes |
fragment |
T4676 |
176-177 |
DT |
denotes |
a |
T4675 |
171-175 |
IN |
denotes |
with |
T4674 |
161-165 |
NN |
denotes |
Acdp |
T4673 |
166-170 |
NN |
denotes |
gene |
T4672 |
156-160 |
DT |
denotes |
each |
T4671 |
152-155 |
IN |
denotes |
for |
T4670 |
140-144 |
VBD |
denotes |
were |
T4669 |
145-151 |
VBN |
denotes |
probed |
T4668 |
132-139 |
NNS |
denotes |
filters |
T4667 |
128-131 |
DT |
denotes |
The |
T4666 |
127-338 |
sentence |
denotes |
The filters were probed for each Acdp gene with a PCR fragment (around 350 bp) from last exon and the 3' untranslated region labeled with α-32P dCTP using the random primer extension system (Life Technologies). |
T4665 |
126-127 |
. |
denotes |
. |
T4664 |
119-126 |
NNP |
denotes |
Origene |
T4663 |
114-118 |
IN |
denotes |
from |
T4662 |
99-103 |
VBD |
denotes |
were |
T4661 |
85-90 |
NN |
denotes |
mouse |
T4660 |
75-84 |
JJ |
denotes |
different |
T4659 |
91-98 |
NNS |
denotes |
tissues |
T4658 |
72-74 |
CD |
denotes |
12 |
T4657 |
61-71 |
VBG |
denotes |
containing |
T4656 |
104-113 |
VBN |
denotes |
purchased |
T4655 |
48-52 |
NN |
denotes |
Blot |
T4654 |
39-47 |
NNP |
denotes |
Northern |
T4653 |
32-38 |
NN |
denotes |
Choice |
T4652 |
53-60 |
NNS |
denotes |
filters |
T4651 |
23-31 |
JJ |
denotes |
Multiple |
T4650 |
22-127 |
sentence |
denotes |
Multiple Choice Northern Blot filters containing 12 different mouse tissues were purchased from Origene. |
T4649 |
14-22 |
NNS |
denotes |
analyses |
T4648 |
9-13 |
NN |
denotes |
blot |
T4647 |
0-8 |
NNP |
denotes |
Northern |
R2774 |
T4647 |
T4648 |
compound |
Northern,blot |
R2775 |
T4648 |
T4649 |
compound |
blot,analyses |
R2776 |
T4651 |
T4652 |
amod |
Multiple,filters |
R2777 |
T4652 |
T4656 |
nsubjpass |
filters,purchased |
R2778 |
T4653 |
T4652 |
compound |
Choice,filters |
R2779 |
T4654 |
T4655 |
compound |
Northern,Blot |
R2780 |
T4655 |
T4652 |
compound |
Blot,filters |
R2781 |
T4657 |
T4652 |
acl |
containing,filters |
R2782 |
T4658 |
T4659 |
nummod |
12,tissues |
R2783 |
T4659 |
T4657 |
dobj |
tissues,containing |
R2784 |
T4660 |
T4659 |
amod |
different,tissues |
R2785 |
T4661 |
T4659 |
compound |
mouse,tissues |
R2786 |
T4662 |
T4656 |
auxpass |
were,purchased |
R2787 |
T4663 |
T4656 |
prep |
from,purchased |
R2788 |
T4664 |
T4663 |
pobj |
Origene,from |
R2789 |
T4665 |
T4656 |
punct |
.,purchased |
R2790 |
T4667 |
T4668 |
det |
The,filters |
R2791 |
T4668 |
T4669 |
nsubjpass |
filters,probed |
R2792 |
T4670 |
T4669 |
auxpass |
were,probed |
R2793 |
T4671 |
T4669 |
prep |
for,probed |
R2794 |
T4672 |
T4673 |
det |
each,gene |
R2795 |
T4673 |
T4671 |
pobj |
gene,for |
R2796 |
T4674 |
T4673 |
compound |
Acdp,gene |
R2797 |
T4675 |
T4669 |
prep |
with,probed |
R2798 |
T4676 |
T4677 |
det |
a,fragment |
R2799 |
T4677 |
T4675 |
pobj |
fragment,with |
R2800 |
T4678 |
T4677 |
compound |
PCR,fragment |
R2801 |
T4679 |
T4680 |
punct |
(,bp |
R2802 |
T4680 |
T4677 |
parataxis |
bp,fragment |
R2803 |
T4681 |
T4682 |
quantmod |
around,350 |
R2804 |
T4682 |
T4680 |
nummod |
350,bp |
R2805 |
T4683 |
T4680 |
punct |
),bp |
R2806 |
T4684 |
T4677 |
prep |
from,fragment |
R2807 |
T4685 |
T4686 |
amod |
last,exon |
R2808 |
T4686 |
T4684 |
pobj |
exon,from |
R2809 |
T4687 |
T4669 |
cc |
and,probed |
R2810 |
T4688 |
T4689 |
det |
the,3 |
R2811 |
T4689 |
T4690 |
nummod |
3,region |
R2812 |
T4690 |
T4693 |
nsubj |
region,labeled |
R2813 |
T4691 |
T4689 |
punct |
',3 |
R2814 |
T4692 |
T4689 |
amod |
untranslated,3 |
R2815 |
T4693 |
T4669 |
conj |
labeled,probed |
R2816 |
T4694 |
T4693 |
prep |
with,labeled |
R2817 |
T4695 |
T4696 |
compound |
α,32P |
R2818 |
T4696 |
T4698 |
compound |
32P,dCTP |
R2819 |
T4697 |
T4696 |
punct |
-,32P |
R2820 |
T4698 |
T4694 |
pobj |
dCTP,with |
R2821 |
T4699 |
T4693 |
advcl |
using,labeled |
R2822 |
T4700 |
T4701 |
det |
the,system |
R2823 |
T4701 |
T4699 |
dobj |
system,using |
R2824 |
T4702 |
T4701 |
amod |
random,system |
R2825 |
T4703 |
T4701 |
compound |
primer,system |
R2826 |
T4704 |
T4701 |
compound |
extension,system |
R2827 |
T4705 |
T4706 |
punct |
(,Technologies |
R2828 |
T4706 |
T4701 |
parataxis |
Technologies,system |
R2829 |
T4707 |
T4706 |
compound |
Life,Technologies |
R2830 |
T4708 |
T4706 |
punct |
),Technologies |
R2831 |
T4709 |
T4693 |
punct |
.,labeled |
R2832 |
T4711 |
T4712 |
nsubjpass |
Hybridizations,carried |
R2833 |
T4713 |
T4712 |
auxpass |
were,carried |
R2834 |
T4714 |
T4712 |
prt |
out,carried |
R2835 |
T4715 |
T4712 |
advmod |
overnight,carried |
R2836 |
T4716 |
T4712 |
punct |
.,carried |
R2837 |
T4718 |
T4719 |
det |
The,filters |
R2838 |
T4719 |
T4720 |
nsubjpass |
filters,washed |
R2839 |
T4721 |
T4720 |
auxpass |
were,washed |
R2840 |
T4722 |
T4720 |
advmod |
twice,washed |
R2841 |
T4723 |
T4720 |
prep |
with,washed |
R2842 |
T4724 |
T4725 |
amod |
washing,buffer |
R2843 |
T4725 |
T4723 |
pobj |
buffer,with |
R2844 |
T4726 |
T4725 |
nummod |
I,buffer |
R2845 |
T4727 |
T4728 |
punct |
(,SDS |
R2846 |
T4728 |
T4725 |
parataxis |
SDS,buffer |
R2847 |
T4729 |
T4730 |
nummod |
2,SSC |
R2848 |
T4730 |
T4728 |
dep |
SSC,SDS |
R2849 |
T4731 |
T4730 |
punct |
×,SSC |
R2850 |
T4732 |
T4728 |
punct |
", ",SDS |
R2851 |
T4733 |
T4734 |
nummod |
0.1,% |
R2852 |
T4734 |
T4728 |
compound |
%,SDS |
R2853 |
T4735 |
T4728 |
punct |
),SDS |
R2854 |
T4736 |
T4720 |
prep |
at,washed |
R2855 |
T4737 |
T4738 |
nummod |
42,°C |
R2856 |
T4738 |
T4736 |
pobj |
°C,at |
R2857 |
T4739 |
T4720 |
prep |
for,washed |
R2858 |
T4740 |
T4741 |
nummod |
15,min |
R2859 |
T4741 |
T4739 |
pobj |
min,for |
R2860 |
T4742 |
T4741 |
punct |
-,min |
R2861 |
T4743 |
T4720 |
punct |
", ",washed |
R2862 |
T4744 |
T4720 |
cc |
and,washed |
R2863 |
T4745 |
T4746 |
advmod |
then,washed |
R2864 |
T4746 |
T4720 |
conj |
washed,washed |
R2865 |
T4747 |
T4746 |
advmod |
twice,washed |
R2866 |
T4748 |
T4746 |
prep |
with,washed |
R2867 |
T4749 |
T4750 |
compound |
washing,buffer |
R2868 |
T4750 |
T4748 |
pobj |
buffer,with |
R2869 |
T4751 |
T4750 |
nummod |
II,buffer |
R2870 |
T4752 |
T4753 |
punct |
(,SDS |
R2871 |
T4753 |
T4750 |
parataxis |
SDS,buffer |
R2872 |
T4754 |
T4755 |
nummod |
0.25,SSC |
R2873 |
T4755 |
T4753 |
dep |
SSC,SDS |
R2874 |
T4756 |
T4755 |
punct |
×,SSC |
R2875 |
T4757 |
T4753 |
punct |
", ",SDS |
R2876 |
T4758 |
T4759 |
nummod |
0.1,% |
R2877 |
T4759 |
T4753 |
compound |
%,SDS |
R2878 |
T4760 |
T4753 |
punct |
),SDS |
R2879 |
T4761 |
T4746 |
prep |
at,washed |
R2880 |
T4762 |
T4763 |
nummod |
65,°C |
R2881 |
T4763 |
T4761 |
pobj |
°C,at |
R2882 |
T4764 |
T4746 |
prep |
for,washed |
R2883 |
T4765 |
T4766 |
nummod |
15,min |
R2884 |
T4766 |
T4764 |
pobj |
min,for |
R2885 |
T4767 |
T4766 |
punct |
-,min |
R2886 |
T4768 |
T4720 |
punct |
.,washed |
R2887 |
T4770 |
T4771 |
amod |
Washed,filters |
R2888 |
T4771 |
T4772 |
nsubjpass |
filters,exposed |
R2889 |
T4773 |
T4772 |
auxpass |
were,exposed |
R2890 |
T4774 |
T4772 |
prep |
to,exposed |
R2891 |
T4775 |
T4776 |
compound |
X-ray,films |
R2892 |
T4776 |
T4774 |
pobj |
films,to |
R2893 |
T4777 |
T4772 |
prep |
for,exposed |
R2894 |
T4778 |
T4777 |
pcomp |
overnight,for |
R2895 |
T4779 |
T4778 |
cc |
or,overnight |
R2896 |
T4780 |
T4778 |
conj |
longer,overnight |
R2897 |
T4781 |
T4782 |
punct |
[,4 |
R2898 |
T4782 |
T4772 |
parataxis |
4,exposed |
R2899 |
T4783 |
T4782 |
nummod |
3,4 |
R2900 |
T4784 |
T4782 |
punct |
",",4 |
R2901 |
T4785 |
T4782 |
punct |
],4 |
R2902 |
T4786 |
T4772 |
punct |
.,exposed |