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PMC:340383 / 18407-18641 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T4538 233-234 . denotes .
T4537 232-233 -RRB- denotes )
T4536 230-232 NN denotes °C
T4535 228-230 CD denotes 62
T4534 227-228 -LRB- denotes (
T4533 205-214 VBG denotes annealing
T4532 215-226 NN denotes temperature
T4531 200-204 JJ denotes high
T4530 197-199 IN denotes at
T4529 184-187 NN denotes PCR
T4528 178-183 NN denotes round
T4527 172-177 JJ denotes first
T4526 188-196 NNS denotes products
T4525 168-171 DT denotes the
T4524 163-167 IN denotes from
T4523 152-157 NN denotes mouse
T4522 143-151 JJ denotes specific
T4521 158-162 NN denotes gene
T4520 139-142 DT denotes the
T4519 131-138 VB denotes amplify
T4518 128-130 TO denotes to
T4517 124-127 RP denotes out
T4516 111-115 RB denotes then
T4515 107-110 VBD denotes was
T4514 92-99 JJ denotes forward
T4513 86-91 JJ denotes human
T4512 81-85 JJ denotes same
T4511 100-106 NN denotes primer
T4510 77-80 DT denotes the
T4509 73-76 CC denotes and
T4508 60-63 NN denotes EST
T4507 54-59 NN denotes mouse
T4506 64-72 NN denotes sequence
T4505 50-53 DT denotes the
T4504 45-49 IN denotes from
T4503 30-37 JJ denotes reverse
T4502 23-29 JJ denotes inside
T4501 38-44 NN denotes primer
T4500 20-22 DT denotes an
T4499 14-19 VBG denotes using
T4498 116-123 VBN denotes carried
T4497 3-9 JJ denotes nested
T4496 10-13 NN denotes PCR
T4495 1-2 DT denotes A
T4494 0-234 sentence denotes A nested PCR using an inside reverse primer from the mouse EST sequence and the same human forward primer was then carried out to amplify the specific mouse gene from the first round PCR products at high annealing temperature (62°C).
R2668 T4495 T4496 det A,PCR
R2669 T4496 T4498 nsubjpass PCR,carried
R2670 T4497 T4496 amod nested,PCR
R2671 T4499 T4496 acl using,PCR
R2672 T4500 T4501 det an,primer
R2673 T4501 T4499 dobj primer,using
R2674 T4502 T4501 amod inside,primer
R2675 T4503 T4501 amod reverse,primer
R2676 T4504 T4501 prep from,primer
R2677 T4505 T4506 det the,sequence
R2678 T4506 T4504 pobj sequence,from
R2679 T4507 T4506 compound mouse,sequence
R2680 T4508 T4506 compound EST,sequence
R2681 T4509 T4501 cc and,primer
R2682 T4510 T4511 det the,primer
R2683 T4511 T4501 conj primer,primer
R2684 T4512 T4511 amod same,primer
R2685 T4513 T4511 amod human,primer
R2686 T4514 T4511 amod forward,primer
R2687 T4515 T4498 auxpass was,carried
R2688 T4516 T4498 advmod then,carried
R2689 T4517 T4498 prt out,carried
R2690 T4518 T4519 aux to,amplify
R2691 T4519 T4498 advcl amplify,carried
R2692 T4520 T4521 det the,gene
R2693 T4521 T4519 dobj gene,amplify
R2694 T4522 T4521 amod specific,gene
R2695 T4523 T4521 compound mouse,gene
R2696 T4524 T4521 prep from,gene
R2697 T4525 T4526 det the,products
R2698 T4526 T4524 pobj products,from
R2699 T4527 T4528 amod first,round
R2700 T4528 T4526 compound round,products
R2701 T4529 T4526 compound PCR,products
R2702 T4530 T4519 prep at,amplify
R2703 T4531 T4532 amod high,temperature
R2704 T4532 T4530 pobj temperature,at
R2705 T4533 T4532 amod annealing,temperature
R2706 T4534 T4532 punct (,temperature
R2707 T4535 T4536 nummod 62,°C
R2708 T4536 T4532 appos °C,temperature
R2709 T4537 T4498 punct ),carried
R2710 T4538 T4498 punct .,carried

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4325 205-214 GO:0097617 denotes annealing
T4324 184-196 SO:0000006 denotes PCR products
T4323 158-162 SO:0000704 denotes gene
T4322 152-157 NCBITaxon:10088 denotes mouse
T4321 92-106 SO:0000121 denotes forward primer
T4320 86-91 NCBITaxon:9606 denotes human
T4319 60-63 SO:0000345 denotes EST
T4318 54-59 NCBITaxon:10088 denotes mouse
T4317 30-44 SO:0000132 denotes reverse primer

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4377 205-214 GO:0097617 denotes annealing
T4376 184-196 SO_EXT:0000006 denotes PCR products
T4375 158-162 SO_EXT:0000704 denotes gene
T4374 152-157 NCBITaxon:10088 denotes mouse
T4373 92-106 SO_EXT:0000121 denotes forward primer
T4372 86-91 NCBITaxon:9606 denotes human
T4371 64-72 SO_EXT:biological_sequence denotes sequence
T4370 60-63 SO_EXT:0000345 denotes EST
T4369 54-59 NCBITaxon:10088 denotes mouse
T4368 30-44 SO_EXT:0000132 denotes reverse primer