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Immunoblotting were carried out using a Western blotting detection system (ECL) (PIERCE). Lane 1, probed with antibody generated by conserved peptide. From top to bottom, each band corresponding to Acdp1 (115 kD), Acdp2 (100 kD), Acdp4 (90 kD) and Acdp3 (80 kD). Lane 2 and 3, probed with the Acdp1 antibodies generated by N-terminal and C-terminal peptides, respectively. Fig. 6B: Immunoblot analysis of Acdp1 in HEK293, 3T3 and PC12 cells. The blots were probed with the antibody against the C-terminus of Acdp-1. Lane 1, 10 μg of HEK293 cell lysates. Lane 2 and 3, 100 μg of 3T3 and PC12 cell lysates."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T6344","span":{"begin":590,"end":596},"obj":"PR:000005643"},{"id":"T6343","span":{"begin":555,"end":563},"obj":"GO:0042571"},{"id":"T6342","span":{"begin":487,"end":492},"obj":"PR:000005643"},{"id":"T6341","span":{"begin":381,"end":391},"obj":"GO:0042571"},{"id":"T6340","span":{"begin":375,"end":380},"obj":"PR:000005643"},{"id":"T6339","span":{"begin":330,"end":335},"obj":"PR:000005645"},{"id":"T6338","span":{"begin":312,"end":317},"obj":"PR:000005646"},{"id":"T6337","span":{"begin":296,"end":301},"obj":"PR:000005644"},{"id":"T6336","span":{"begin":280,"end":285},"obj":"PR:000005643"},{"id":"T6335","span":{"begin":192,"end":200},"obj":"GO:0042571"},{"id":"T6334","span":{"begin":65,"end":71},"obj":"UBERON:0000479"},{"id":"T6333","span":{"begin":59,"end":64},"obj":"UBERON:0000955"}],"text":"Figure 6 Fig. 6A: Immunoblot analysis of Acdp proteins in brain tissue extracts. Immunoblotting were carried out using a Western blotting detection system (ECL) (PIERCE). Lane 1, probed with antibody generated by conserved peptide. From top to bottom, each band corresponding to Acdp1 (115 kD), Acdp2 (100 kD), Acdp4 (90 kD) and Acdp3 (80 kD). Lane 2 and 3, probed with the Acdp1 antibodies generated by N-terminal and C-terminal peptides, respectively. Fig. 6B: Immunoblot analysis of Acdp1 in HEK293, 3T3 and PC12 cells. The blots were probed with the antibody against the C-terminus of Acdp-1. Lane 1, 10 μg of HEK293 cell lysates. Lane 2 and 3, 100 μg of 3T3 and PC12 cell lysates."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T6367","span":{"begin":673,"end":677},"obj":"CL_GO_EXT:cell"},{"id":"T6366","span":{"begin":622,"end":626},"obj":"CL_GO_EXT:cell"},{"id":"T6365","span":{"begin":590,"end":596},"obj":"PR_EXT:000005643"},{"id":"T6364","span":{"begin":576,"end":586},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T6363","span":{"begin":555,"end":563},"obj":"GO:0042571"},{"id":"T6362","span":{"begin":517,"end":522},"obj":"CL_GO_EXT:cell"},{"id":"T6361","span":{"begin":487,"end":492},"obj":"PR_EXT:000005643"},{"id":"T6360","span":{"begin":431,"end":439},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T6359","span":{"begin":420,"end":430},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T6358","span":{"begin":405,"end":415},"obj":"CHEBI_SO_EXT:N_terminus_or_N_terminal_region"},{"id":"T6357","span":{"begin":381,"end":391},"obj":"GO:0042571"},{"id":"T6356","span":{"begin":375,"end":380},"obj":"PR_EXT:000005643"},{"id":"T6355","span":{"begin":330,"end":335},"obj":"PR_EXT:000005645"},{"id":"T6354","span":{"begin":312,"end":317},"obj":"PR_EXT:000005646"},{"id":"T6353","span":{"begin":296,"end":301},"obj":"PR_EXT:000005644"},{"id":"T6352","span":{"begin":280,"end":285},"obj":"PR_EXT:000005643"},{"id":"T6351","span":{"begin":224,"end":231},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T6350","span":{"begin":214,"end":223},"obj":"SO_EXT:biological_conservation_process_or_quality"},{"id":"T6349","span":{"begin":192,"end":200},"obj":"GO:0042571"},{"id":"T6348","span":{"begin":65,"end":71},"obj":"UBERON:0000479"},{"id":"T6347","span":{"begin":59,"end":64},"obj":"UBERON:0000955"},{"id":"T6346","span":{"begin":47,"end":55},"obj":"CHEBI_PR_EXT:protein"},{"id":"T6345","span":{"begin":42,"end":46},"obj":"PR_EXT:ACDP_or_CNNM"}],"text":"Figure 6 Fig. 6A: Immunoblot analysis of Acdp proteins in brain tissue extracts. Immunoblotting were carried out using a Western blotting detection system (ECL) (PIERCE). Lane 1, probed with antibody generated by conserved peptide. From top to bottom, each band corresponding to Acdp1 (115 kD), Acdp2 (100 kD), Acdp4 (90 kD) and Acdp3 (80 kD). Lane 2 and 3, probed with the Acdp1 antibodies generated by N-terminal and C-terminal peptides, respectively. Fig. 6B: Immunoblot analysis of Acdp1 in HEK293, 3T3 and PC12 cells. The blots were probed with the antibody against the C-terminus of Acdp-1. Lane 1, 10 μg of HEK293 cell lysates. Lane 2 and 3, 100 μg of 3T3 and PC12 cell lysates."}