Id |
Subject |
Object |
Predicate |
Lexical cue |
T3159 |
1494-1495 |
. |
denotes |
. |
T3158 |
1493-1494 |
CD |
denotes |
1 |
T3157 |
1492-1493 |
HYPH |
denotes |
- |
T3156 |
1488-1492 |
NN |
denotes |
Acdp |
T3155 |
1464-1476 |
RB |
denotes |
specifically |
T3154 |
1454-1455 |
HYPH |
denotes |
- |
T3153 |
1453-1454 |
NN |
denotes |
C |
T3152 |
1455-1463 |
NN |
denotes |
terminus |
T3151 |
1447-1452 |
NN |
denotes |
Acdp1 |
T3150 |
1439-1446 |
IN |
denotes |
against |
T3149 |
1430-1438 |
NN |
denotes |
antibody |
T3148 |
1426-1429 |
DT |
denotes |
the |
T3147 |
1477-1487 |
VBZ |
denotes |
recognizes |
T3146 |
1421-1425 |
IN |
denotes |
that |
T3145 |
1405-1411 |
NN |
denotes |
tissue |
T3144 |
1412-1420 |
NNS |
denotes |
extracts |
T3143 |
1399-1404 |
NN |
denotes |
brain |
T3142 |
1396-1398 |
IN |
denotes |
of |
T3141 |
1387-1395 |
NN |
denotes |
analysis |
T3140 |
1383-1386 |
PRP$ |
denotes |
our |
T3139 |
1356-1366 |
NN |
denotes |
immunoblot |
T3138 |
1367-1374 |
NNS |
denotes |
results |
T3137 |
1350-1355 |
DT |
denotes |
these |
T3136 |
1348-1350 |
, |
denotes |
, |
T3135 |
1375-1382 |
VBP |
denotes |
support |
T3134 |
1336-1348 |
RB |
denotes |
Nevertheless |
T3133 |
1335-1495 |
sentence |
denotes |
Nevertheless, these immunoblot results support our analysis of brain tissue extracts that the antibody against Acdp1 C-terminus specifically recognizes Acdp-1. |
T3132 |
1334-1335 |
. |
denotes |
. |
T3131 |
1329-1334 |
NNS |
denotes |
cells |
T3130 |
1322-1328 |
NN |
denotes |
HEK293 |
T3129 |
1319-1321 |
IN |
denotes |
in |
T3128 |
1300-1307 |
JJS |
denotes |
highest |
T3127 |
1308-1318 |
NN |
denotes |
expression |
T3126 |
1296-1299 |
DT |
denotes |
the |
T3125 |
1291-1295 |
IN |
denotes |
with |
T3124 |
1289-1291 |
, |
denotes |
, |
T3123 |
1286-1289 |
NN |
denotes |
lot |
T3122 |
1284-1285 |
DT |
denotes |
a |
T3121 |
1268-1272 |
NN |
denotes |
cell |
T3120 |
1273-1278 |
NNS |
denotes |
types |
T3119 |
1262-1267 |
DT |
denotes |
these |
T3118 |
1259-1261 |
IN |
denotes |
in |
T3117 |
1253-1258 |
NN |
denotes |
Acdp1 |
T3116 |
1250-1252 |
IN |
denotes |
of |
T3115 |
1232-1242 |
NN |
denotes |
expression |
T3114 |
1243-1249 |
NNS |
denotes |
levels |
T3113 |
1228-1231 |
DT |
denotes |
the |
T3112 |
1226-1228 |
, |
denotes |
, |
T3111 |
1279-1283 |
VBP |
denotes |
vary |
T3110 |
1222-1226 |
RB |
denotes |
Thus |
T3109 |
1221-1335 |
sentence |
denotes |
Thus, the expression levels of Acdp1 in these cell types vary a lot, with the highest expression in HEK293 cells. |
T3108 |
1220-1221 |
. |
denotes |
. |
T3107 |
1208-1212 |
NN |
denotes |
cell |
T3106 |
1203-1207 |
NN |
denotes |
PC12 |
T3105 |
1199-1202 |
CC |
denotes |
and |
T3104 |
1213-1220 |
NNS |
denotes |
lysates |
T3103 |
1195-1198 |
NN |
denotes |
3T3 |
T3102 |
1192-1194 |
IN |
denotes |
of |
T3101 |
1183-1191 |
NNS |
denotes |
extracts |
T3100 |
1180-1182 |
NN |
denotes |
μg |
T3099 |
1176-1179 |
CD |
denotes |
100 |
T3098 |
1174-1176 |
, |
denotes |
, |
T3097 |
1162-1166 |
NN |
denotes |
cell |
T3096 |
1167-1174 |
NNS |
denotes |
lysates |
T3095 |
1155-1161 |
NN |
denotes |
HEK293 |
T3094 |
1152-1154 |
IN |
denotes |
of |
T3093 |
1143-1151 |
NNS |
denotes |
extracts |
T3092 |
1140-1142 |
NN |
denotes |
μg |
T3091 |
1137-1139 |
CD |
denotes |
10 |
T3090 |
1134-1136 |
IN |
denotes |
of |
T3089 |
1126-1133 |
NNS |
denotes |
signals |
T3088 |
1119-1121 |
CD |
denotes |
6B |
T3087 |
1114-1118 |
NN |
denotes |
Fig. |
T3086 |
1111-1113 |
IN |
denotes |
in |
T3085 |
1105-1110 |
VBN |
denotes |
shown |
T3084 |
1103-1105 |
, |
denotes |
, |
T3083 |
1099-1103 |
NN |
denotes |
note |
T3082 |
1122-1125 |
VBP |
denotes |
are |
T3081 |
1096-1098 |
IN |
denotes |
Of |
T3080 |
1095-1221 |
sentence |
denotes |
Of note, shown in Fig. 6B are signals of 10 μg extracts of HEK293 cell lysates, 100 μg extracts of 3T3 and PC12 cell lysates. |
T3079 |
1094-1095 |
. |
denotes |
. |
T3078 |
1082-1086 |
NN |
denotes |
cell |
T3077 |
1087-1094 |
NNS |
denotes |
lysates |
T3076 |
1078-1081 |
DT |
denotes |
all |
T3075 |
1075-1077 |
IN |
denotes |
in |
T3074 |
1069-1074 |
NN |
denotes |
Acdp1 |
T3073 |
1066-1068 |
IN |
denotes |
of |
T3072 |
1052-1060 |
JJ |
denotes |
specific |
T3071 |
1061-1065 |
NN |
denotes |
band |
T3070 |
1050-1051 |
DT |
denotes |
a |
T3069 |
1032-1040 |
NN |
denotes |
antibody |
T3068 |
1027-1031 |
DT |
denotes |
this |
T3067 |
1025-1027 |
, |
denotes |
, |
T3066 |
1041-1049 |
VBD |
denotes |
detected |
T3065 |
1015-1025 |
RB |
denotes |
Apparently |
T3064 |
1014-1095 |
sentence |
denotes |
Apparently, this antibody detected a specific band of Acdp1 in all cell lysates. |
T3063 |
1013-1014 |
. |
denotes |
. |
T3062 |
1011-1013 |
CD |
denotes |
6B |
T3061 |
1006-1010 |
NN |
denotes |
Fig. |
T3060 |
1003-1005 |
IN |
denotes |
in |
T3059 |
993-996 |
VBP |
denotes |
are |
T3058 |
997-1002 |
VBN |
denotes |
shown |
T3057 |
985-992 |
NNS |
denotes |
results |
T3056 |
981-984 |
DT |
denotes |
The |
T3055 |
980-1014 |
sentence |
denotes |
The results are shown in Fig. 6B. |
T3054 |
979-980 |
. |
denotes |
. |
T3053 |
969-973 |
NN |
denotes |
PC12 |
T3052 |
965-968 |
CC |
denotes |
and |
T3051 |
961-964 |
NN |
denotes |
3T3 |
T3050 |
959-961 |
, |
denotes |
, |
T3049 |
974-979 |
NNS |
denotes |
cells |
T3048 |
953-959 |
NN |
denotes |
HEK293 |
T3047 |
950-952 |
IN |
denotes |
of |
T3046 |
941-949 |
NNS |
denotes |
extracts |
T3045 |
929-931 |
PRP |
denotes |
we |
T3044 |
927-929 |
, |
denotes |
, |
T3043 |
918-919 |
HYPH |
denotes |
- |
T3042 |
917-918 |
NN |
denotes |
C |
T3041 |
911-916 |
NN |
denotes |
Acdp1 |
T3040 |
919-927 |
NN |
denotes |
terminus |
T3039 |
907-910 |
DT |
denotes |
the |
T3038 |
899-906 |
IN |
denotes |
against |
T3037 |
890-898 |
NN |
denotes |
antibody |
T3036 |
886-889 |
DT |
denotes |
the |
T3035 |
883-885 |
IN |
denotes |
of |
T3034 |
871-882 |
NN |
denotes |
specificity |
T3033 |
867-870 |
DT |
denotes |
the |
T3032 |
932-940 |
VBD |
denotes |
analyzed |
T3031 |
849-856 |
RB |
denotes |
further |
T3030 |
857-866 |
VB |
denotes |
determine |
T3029 |
846-848 |
TO |
denotes |
To |
T3028 |
845-980 |
sentence |
denotes |
To further determine the specificity of the antibody against the Acdp1 C-terminus, we analyzed extracts of HEK293, 3T3 and PC12 cells. |
T3027 |
844-845 |
. |
denotes |
. |
T3026 |
843-844 |
-RRB- |
denotes |
) |
T3025 |
842-843 |
CD |
denotes |
1 |
T3024 |
835-837 |
, |
denotes |
, |
T3023 |
833-835 |
CD |
denotes |
6A |
T3022 |
828-832 |
NN |
denotes |
Fig. |
T3021 |
837-841 |
NN |
denotes |
lane |
T3020 |
827-828 |
-LRB- |
denotes |
( |
T3019 |
813-817 |
NN |
denotes |
Acdp |
T3018 |
818-826 |
NN |
denotes |
proteins |
T3017 |
809-812 |
DT |
denotes |
all |
T3016 |
783-791 |
NN |
denotes |
sequence |
T3015 |
773-782 |
VBN |
denotes |
conserved |
T3014 |
792-799 |
NN |
denotes |
peptide |
T3013 |
769-772 |
DT |
denotes |
the |
T3012 |
766-768 |
IN |
denotes |
by |
T3011 |
757-765 |
VBN |
denotes |
produced |
T3010 |
748-756 |
NN |
denotes |
antibody |
T3009 |
744-747 |
DT |
denotes |
the |
T3008 |
742-744 |
, |
denotes |
, |
T3007 |
800-808 |
VBD |
denotes |
detected |
T3006 |
734-742 |
VBN |
denotes |
expected |
T3005 |
731-733 |
IN |
denotes |
As |
T3004 |
730-845 |
sentence |
denotes |
As expected, the antibody produced by the conserved sequence peptide detected all Acdp proteins (Fig. 6A, lane 1). |
T3003 |
729-730 |
. |
denotes |
. |
T3002 |
728-729 |
-RRB- |
denotes |
) |
T3001 |
727-728 |
CD |
denotes |
2 |
T3000 |
720-722 |
, |
denotes |
, |
T2999 |
718-720 |
CD |
denotes |
6A |
T2998 |
713-717 |
NN |
denotes |
Fig. |
T2997 |
722-726 |
NN |
denotes |
lane |
T2996 |
712-713 |
-LRB- |
denotes |
( |
T2995 |
705-711 |
JJR |
denotes |
higher |
T2994 |
691-704 |
RB |
denotes |
significantly |
T2993 |
680-686 |
NN |
denotes |
latter |
T2992 |
677-679 |
IN |
denotes |
to |
T2991 |
666-676 |
NN |
denotes |
reactivity |
T2990 |
662-665 |
DT |
denotes |
the |
T2989 |
687-690 |
VBD |
denotes |
was |
T2988 |
653-661 |
IN |
denotes |
although |
T2987 |
651-653 |
, |
denotes |
, |
T2986 |
646-651 |
NN |
denotes |
Acdp1 |
T2985 |
643-645 |
IN |
denotes |
to |
T2984 |
634-642 |
NN |
denotes |
addition |
T2978 |
595-596 |
NN |
denotes |
N |
T2977 |
592-594 |
IN |
denotes |
by |
T2976 |
582-591 |
VBN |
denotes |
generated |
T2975 |
614-624 |
VBD |
denotes |
recognized |
T2974 |
573-581 |
NN |
denotes |
antibody |
T2973 |
569-572 |
DT |
denotes |
The |
T2972 |
568-730 |
sentence |
denotes |
The antibody generated by N-terminal peptide recognized Acdp4 in addition to Acdp1, although the reactivity to latter was significantly higher (Fig. 6A, lane 2). |
T2971 |
567-568 |
. |
denotes |
. |
T2970 |
566-567 |
-RRB- |
denotes |
) |
T2969 |
565-566 |
CD |
denotes |
3 |
T2968 |
560-564 |
NN |
denotes |
lane |
T2967 |
559-560 |
-LRB- |
denotes |
( |
T2966 |
553-558 |
NN |
denotes |
Acdp1 |
T2965 |
531-543 |
RB |
denotes |
specifically |
T2964 |
514-522 |
JJ |
denotes |
terminal |
T2963 |
513-514 |
HYPH |
denotes |
- |
T2962 |
523-530 |
NN |
denotes |
peptide |
T2961 |
512-513 |
NN |
denotes |
C |
T2960 |
509-511 |
IN |
denotes |
by |
T2959 |
500-508 |
VBN |
denotes |
produced |
T2958 |
491-499 |
NN |
denotes |
antibody |
T2957 |
487-490 |
DT |
denotes |
the |
T2956 |
485-487 |
, |
denotes |
, |
T2955 |
483-485 |
CD |
denotes |
6A |
T2954 |
478-482 |
NN |
denotes |
Fig. |
T2953 |
475-477 |
IN |
denotes |
in |
T2952 |
544-552 |
VBD |
denotes |
detected |
T2951 |
469-474 |
VBN |
denotes |
shown |
T2950 |
466-468 |
IN |
denotes |
As |
T2949 |
465-568 |
sentence |
denotes |
As shown in Fig. 6A, the antibody produced by C-terminal peptide specifically detected Acdp1 (lane 3). |
T2948 |
464-465 |
. |
denotes |
. |
T2947 |
456-464 |
NNS |
denotes |
extracts |
T2946 |
449-455 |
NN |
denotes |
tissue |
T2945 |
443-448 |
NN |
denotes |
brain |
T2944 |
437-442 |
NN |
denotes |
mouse |
T2943 |
434-436 |
IN |
denotes |
of |
T2942 |
425-433 |
NN |
denotes |
analysis |
T2941 |
419-420 |
HYPH |
denotes |
- |
T2940 |
420-424 |
NN |
denotes |
blot |
T2939 |
412-419 |
NNP |
denotes |
Western |
T2938 |
399-401 |
PRP |
denotes |
we |
T2937 |
397-399 |
, |
denotes |
, |
T2936 |
387-397 |
NNS |
denotes |
antibodies |
T2935 |
383-386 |
DT |
denotes |
the |
T2934 |
380-382 |
IN |
denotes |
of |
T2933 |
368-379 |
NN |
denotes |
specificity |
T2932 |
364-367 |
DT |
denotes |
the |
T2931 |
402-411 |
VBD |
denotes |
conducted |
T2930 |
359-363 |
VB |
denotes |
test |
T2929 |
356-358 |
TO |
denotes |
To |
T2928 |
355-465 |
sentence |
denotes |
To test the specificity of the antibodies, we conducted Western-blot analysis of mouse brain tissue extracts. |
T2927 |
354-355 |
. |
denotes |
. |
T2926 |
347-354 |
NNS |
denotes |
rabbits |
T2925 |
336-346 |
VBG |
denotes |
immunizing |
T2924 |
333-335 |
IN |
denotes |
by |
T2923 |
319-323 |
VBD |
denotes |
were |
T2922 |
311-318 |
NN |
denotes |
peptide |
T2921 |
306-310 |
DT |
denotes |
each |
T2920 |
302-305 |
IN |
denotes |
for |
T2919 |
324-332 |
VBN |
denotes |
produced |
T2918 |
291-301 |
NNS |
denotes |
antibodies |
T2917 |
280-290 |
JJ |
denotes |
polyclonal |
T2916 |
276-279 |
CC |
denotes |
and |
T2915 |
268-275 |
NNP |
denotes |
Genosys |
T2914 |
262-267 |
NNP |
denotes |
Sigma |
T2913 |
257-261 |
IN |
denotes |
from |
T2912 |
248-256 |
NN |
denotes |
software |
T2911 |
245-247 |
IN |
denotes |
by |
T2910 |
230-234 |
VBD |
denotes |
were |
T2909 |
235-244 |
VBN |
denotes |
predicted |
T2908 |
214-223 |
JJ |
denotes |
antigenic |
T2907 |
224-229 |
NNS |
denotes |
sites |
T2906 |
208-213 |
DT |
denotes |
These |
T2905 |
207-355 |
sentence |
denotes |
These antigenic sites were predicted by software from Sigma Genosys and polyclonal antibodies for each peptide were produced by immunizing rabbits. |
T2904 |
206-207 |
. |
denotes |
. |
T2903 |
205-206 |
-RRB- |
denotes |
) |
T2902 |
192-197 |
NNP |
denotes |
Sigma |
T2901 |
198-205 |
NNP |
denotes |
Genosys |
T2900 |
191-192 |
-LRB- |
denotes |
( |
T2899 |
166-178 |
RB |
denotes |
commercially |
T2898 |
161-165 |
VBD |
denotes |
were |
T2897 |
159-160 |
-RRB- |
denotes |
) |
T2896 |
130-159 |
NN |
denotes |
HNIVDILFVKDLAFVDPDDCTPLLTVTRF |
T2895 |
129-130 |
-LRB- |
denotes |
( |
T2894 |
123-128 |
NN |
denotes |
Acdp1 |
T2893 |
120-122 |
IN |
denotes |
of |
T2892 |
113-119 |
NN |
denotes |
domain |
T2891 |
109-112 |
NN |
denotes |
ACD |
T2890 |
104-108 |
IN |
denotes |
from |
T2889 |
96-103 |
NN |
denotes |
peptide |
T2888 |
86-95 |
JJ |
denotes |
conserved |
T2887 |
84-86 |
, |
denotes |
, |
T2886 |
72-73 |
-RRB- |
denotes |
) |
T2885 |
58-72 |
NN |
denotes |
TQQLTLSPAAVPTR |
T2884 |
57-58 |
-LRB- |
denotes |
( |
T2883 |
55-56 |
HYPH |
denotes |
- |
T2882 |
54-55 |
NN |
denotes |
C |
T2881 |
50-53 |
CC |
denotes |
and |
T2880 |
48-49 |
-RRB- |
denotes |
) |
T2879 |
24-48 |
NN |
denotes |
TSFLLRVYFQPGPPATAAPVPSPT |
T2878 |
23-24 |
-LRB- |
denotes |
( |
T2877 |
21-22 |
HYPH |
denotes |
- |
T2876 |
20-21 |
NN |
denotes |
N |
T2875 |
74-84 |
NNS |
denotes |
terminuses |
T2874 |
14-19 |
NN |
denotes |
Acdp1 |
T2873 |
9-13 |
IN |
denotes |
from |
T2872 |
179-190 |
VBN |
denotes |
synthesized |
T2871 |
0-8 |
NNS |
denotes |
Peptides |
T2983 |
631-633 |
IN |
denotes |
in |
T2982 |
625-630 |
NN |
denotes |
Acdp4 |
T2981 |
597-605 |
JJ |
denotes |
terminal |
T2980 |
596-597 |
HYPH |
denotes |
- |
T2979 |
606-613 |
NN |
denotes |
peptide |
R1579 |
T2871 |
T2872 |
nsubjpass |
Peptides,synthesized |
R1580 |
T2873 |
T2871 |
prep |
from,Peptides |
R1581 |
T2874 |
T2875 |
nmod |
Acdp1,terminuses |
R1582 |
T2875 |
T2873 |
pobj |
terminuses,from |
R1583 |
T2876 |
T2875 |
nmod |
N,terminuses |
R1584 |
T2877 |
T2876 |
punct |
-,N |
R1585 |
T2878 |
T2876 |
punct |
(,N |
R1586 |
T2879 |
T2876 |
appos |
TSFLLRVYFQPGPPATAAPVPSPT,N |
R1587 |
T2880 |
T2876 |
punct |
),N |
R1588 |
T2881 |
T2876 |
cc |
and,N |
R1589 |
T2882 |
T2876 |
conj |
C,N |
R1590 |
T2883 |
T2882 |
punct |
-,C |
R1591 |
T2884 |
T2882 |
punct |
(,C |
R1592 |
T2885 |
T2882 |
appos |
TQQLTLSPAAVPTR,C |
R1593 |
T2886 |
T2875 |
punct |
),terminuses |
R1594 |
T2887 |
T2871 |
punct |
", ",Peptides |
R1595 |
T2888 |
T2889 |
amod |
conserved,peptide |
R1596 |
T2889 |
T2871 |
appos |
peptide,Peptides |
R1597 |
T2890 |
T2889 |
prep |
from,peptide |
R1598 |
T2891 |
T2892 |
compound |
ACD,domain |
R1599 |
T2892 |
T2890 |
pobj |
domain,from |
R1600 |
T2893 |
T2892 |
prep |
of,domain |
R1601 |
T2894 |
T2893 |
pobj |
Acdp1,of |
R1602 |
T2895 |
T2894 |
punct |
(,Acdp1 |
R1603 |
T2896 |
T2894 |
appos |
HNIVDILFVKDLAFVDPDDCTPLLTVTRF,Acdp1 |
R1604 |
T2897 |
T2872 |
punct |
),synthesized |
R1605 |
T2898 |
T2872 |
auxpass |
were,synthesized |
R1606 |
T2899 |
T2872 |
advmod |
commercially,synthesized |
R1607 |
T2900 |
T2901 |
punct |
(,Genosys |
R1608 |
T2901 |
T2872 |
parataxis |
Genosys,synthesized |
R1609 |
T2902 |
T2901 |
compound |
Sigma,Genosys |
R1610 |
T2903 |
T2901 |
punct |
),Genosys |
R1611 |
T2904 |
T2872 |
punct |
.,synthesized |
R1612 |
T2906 |
T2907 |
det |
These,sites |
R1613 |
T2907 |
T2909 |
nsubjpass |
sites,predicted |
R1614 |
T2908 |
T2907 |
amod |
antigenic,sites |
R1615 |
T2910 |
T2909 |
auxpass |
were,predicted |
R1616 |
T2911 |
T2909 |
prep |
by,predicted |
R1617 |
T2912 |
T2911 |
pobj |
software,by |
R1618 |
T2913 |
T2912 |
prep |
from,software |
R1619 |
T2914 |
T2915 |
compound |
Sigma,Genosys |
R1620 |
T2915 |
T2913 |
pobj |
Genosys,from |
R1621 |
T2916 |
T2909 |
cc |
and,predicted |
R1622 |
T2917 |
T2918 |
amod |
polyclonal,antibodies |
R1623 |
T2918 |
T2919 |
nsubjpass |
antibodies,produced |
R1624 |
T2919 |
T2909 |
conj |
produced,predicted |
R1625 |
T2920 |
T2918 |
prep |
for,antibodies |
R1626 |
T2921 |
T2922 |
det |
each,peptide |
R1627 |
T2922 |
T2920 |
pobj |
peptide,for |
R1628 |
T2923 |
T2919 |
auxpass |
were,produced |
R1629 |
T2924 |
T2919 |
prep |
by,produced |
R1630 |
T2925 |
T2924 |
pcomp |
immunizing,by |
R1631 |
T2926 |
T2925 |
dobj |
rabbits,immunizing |
R1632 |
T2927 |
T2919 |
punct |
.,produced |
R1633 |
T2929 |
T2930 |
aux |
To,test |
R1634 |
T2930 |
T2931 |
advcl |
test,conducted |
R1635 |
T2932 |
T2933 |
det |
the,specificity |
R1636 |
T2933 |
T2930 |
dobj |
specificity,test |
R1637 |
T2934 |
T2933 |
prep |
of,specificity |
R1638 |
T2935 |
T2936 |
det |
the,antibodies |
R1639 |
T2936 |
T2934 |
pobj |
antibodies,of |
R1640 |
T2937 |
T2931 |
punct |
", ",conducted |
R1641 |
T2938 |
T2931 |
nsubj |
we,conducted |
R1642 |
T2939 |
T2940 |
compound |
Western,blot |
R1643 |
T2940 |
T2942 |
compound |
blot,analysis |
R1644 |
T2941 |
T2940 |
punct |
-,blot |
R1645 |
T2942 |
T2931 |
dobj |
analysis,conducted |
R1646 |
T2943 |
T2942 |
prep |
of,analysis |
R1647 |
T2944 |
T2945 |
compound |
mouse,brain |
R1648 |
T2945 |
T2946 |
compound |
brain,tissue |
R1649 |
T2946 |
T2947 |
compound |
tissue,extracts |
R1650 |
T2947 |
T2943 |
pobj |
extracts,of |
R1651 |
T2948 |
T2931 |
punct |
.,conducted |
R1652 |
T2950 |
T2951 |
mark |
As,shown |
R1653 |
T2951 |
T2952 |
advcl |
shown,detected |
R1654 |
T2953 |
T2951 |
prep |
in,shown |
R1655 |
T2954 |
T2953 |
pobj |
Fig.,in |
R1656 |
T2955 |
T2954 |
nummod |
6A,Fig. |
R1657 |
T2956 |
T2952 |
punct |
", ",detected |
R1658 |
T2957 |
T2958 |
det |
the,antibody |
R1659 |
T2958 |
T2952 |
nsubj |
antibody,detected |
R1660 |
T2959 |
T2958 |
acl |
produced,antibody |
R1661 |
T2960 |
T2959 |
prep |
by,produced |
R1662 |
T2961 |
T2962 |
nmod |
C,peptide |
R1663 |
T2962 |
T2960 |
pobj |
peptide,by |
R1664 |
T2963 |
T2961 |
punct |
-,C |
R1665 |
T2964 |
T2961 |
amod |
terminal,C |
R1666 |
T2965 |
T2952 |
advmod |
specifically,detected |
R1667 |
T2966 |
T2952 |
dobj |
Acdp1,detected |
R1668 |
T2967 |
T2968 |
punct |
(,lane |
R1669 |
T2968 |
T2952 |
parataxis |
lane,detected |
R1670 |
T2969 |
T2968 |
nummod |
3,lane |
R1671 |
T2970 |
T2968 |
punct |
),lane |
R1672 |
T2971 |
T2952 |
punct |
.,detected |
R1673 |
T2973 |
T2974 |
det |
The,antibody |
R1674 |
T2974 |
T2975 |
nsubj |
antibody,recognized |
R1675 |
T2976 |
T2974 |
acl |
generated,antibody |
R1676 |
T2977 |
T2976 |
agent |
by,generated |
R1677 |
T2978 |
T2979 |
nmod |
N,peptide |
R1678 |
T2979 |
T2977 |
pobj |
peptide,by |
R1679 |
T2980 |
T2978 |
punct |
-,N |
R1680 |
T2981 |
T2978 |
amod |
terminal,N |
R1681 |
T2982 |
T2975 |
dobj |
Acdp4,recognized |
R1682 |
T2983 |
T2975 |
prep |
in,recognized |
R1683 |
T2984 |
T2983 |
pobj |
addition,in |
R1684 |
T2985 |
T2984 |
prep |
to,addition |
R1685 |
T2986 |
T2985 |
pobj |
Acdp1,to |
R1686 |
T2987 |
T2975 |
punct |
", ",recognized |
R1687 |
T2988 |
T2989 |
mark |
although,was |
R1688 |
T2989 |
T2975 |
advcl |
was,recognized |
R1689 |
T2990 |
T2991 |
det |
the,reactivity |
R1690 |
T2991 |
T2989 |
nsubj |
reactivity,was |
R1691 |
T2992 |
T2991 |
prep |
to,reactivity |
R1692 |
T2993 |
T2992 |
pobj |
latter,to |
R1693 |
T2994 |
T2995 |
advmod |
significantly,higher |
R1694 |
T2995 |
T2989 |
acomp |
higher,was |
R1695 |
T2996 |
T2997 |
punct |
(,lane |
R1696 |
T2997 |
T2975 |
parataxis |
lane,recognized |
R1697 |
T2998 |
T2997 |
dep |
Fig.,lane |
R1698 |
T2999 |
T2998 |
nummod |
6A,Fig. |
R1699 |
T3000 |
T2997 |
punct |
", ",lane |
R1700 |
T3001 |
T2997 |
nummod |
2,lane |
R1701 |
T3002 |
T2997 |
punct |
),lane |
R1702 |
T3003 |
T2975 |
punct |
.,recognized |
R1703 |
T3005 |
T3006 |
mark |
As,expected |
R1704 |
T3006 |
T3007 |
advcl |
expected,detected |
R1705 |
T3008 |
T3007 |
punct |
", ",detected |
R1706 |
T3009 |
T3010 |
det |
the,antibody |
R1707 |
T3010 |
T3007 |
nsubj |
antibody,detected |
R1708 |
T3011 |
T3010 |
acl |
produced,antibody |
R1709 |
T3012 |
T3011 |
agent |
by,produced |
R1710 |
T3013 |
T3014 |
det |
the,peptide |
R1711 |
T3014 |
T3012 |
pobj |
peptide,by |
R1712 |
T3015 |
T3014 |
amod |
conserved,peptide |
R1713 |
T3016 |
T3014 |
compound |
sequence,peptide |
R1714 |
T3017 |
T3018 |
det |
all,proteins |
R1715 |
T3018 |
T3007 |
dobj |
proteins,detected |
R1716 |
T3019 |
T3018 |
compound |
Acdp,proteins |
R1717 |
T3020 |
T3021 |
punct |
(,lane |
R1718 |
T3021 |
T3007 |
parataxis |
lane,detected |
R1719 |
T3022 |
T3021 |
dep |
Fig.,lane |
R1720 |
T3023 |
T3022 |
nummod |
6A,Fig. |
R1721 |
T3024 |
T3021 |
punct |
", ",lane |
R1722 |
T3025 |
T3021 |
nummod |
1,lane |
R1723 |
T3026 |
T3021 |
punct |
),lane |
R1724 |
T3027 |
T3007 |
punct |
.,detected |
R1725 |
T3029 |
T3030 |
aux |
To,determine |
R1726 |
T3030 |
T3032 |
advcl |
determine,analyzed |
R1727 |
T3031 |
T3030 |
advmod |
further,determine |
R1728 |
T3033 |
T3034 |
det |
the,specificity |
R1729 |
T3034 |
T3030 |
dobj |
specificity,determine |
R1730 |
T3035 |
T3034 |
prep |
of,specificity |
R1731 |
T3036 |
T3037 |
det |
the,antibody |
R1732 |
T3037 |
T3035 |
pobj |
antibody,of |
R1733 |
T3038 |
T3034 |
prep |
against,specificity |
R1734 |
T3039 |
T3040 |
det |
the,terminus |
R1735 |
T3040 |
T3038 |
pobj |
terminus,against |
R1736 |
T3041 |
T3040 |
compound |
Acdp1,terminus |
R1737 |
T3042 |
T3040 |
compound |
C,terminus |
R1738 |
T3043 |
T3040 |
punct |
-,terminus |
R1739 |
T3044 |
T3032 |
punct |
", ",analyzed |
R1740 |
T3045 |
T3032 |
nsubj |
we,analyzed |
R1741 |
T3046 |
T3032 |
dobj |
extracts,analyzed |
R1742 |
T3047 |
T3046 |
prep |
of,extracts |
R1743 |
T3048 |
T3049 |
nmod |
HEK293,cells |
R1744 |
T3049 |
T3047 |
pobj |
cells,of |
R1745 |
T3050 |
T3048 |
punct |
", ",HEK293 |
R1746 |
T3051 |
T3048 |
conj |
3T3,HEK293 |
R1747 |
T3052 |
T3051 |
cc |
and,3T3 |
R1748 |
T3053 |
T3051 |
conj |
PC12,3T3 |
R1749 |
T3054 |
T3032 |
punct |
.,analyzed |
R1750 |
T3056 |
T3057 |
det |
The,results |
R1751 |
T3057 |
T3058 |
nsubjpass |
results,shown |
R1752 |
T3059 |
T3058 |
auxpass |
are,shown |
R1753 |
T3060 |
T3058 |
prep |
in,shown |
R1754 |
T3061 |
T3060 |
pobj |
Fig.,in |
R1755 |
T3062 |
T3061 |
nummod |
6B,Fig. |
R1756 |
T3063 |
T3058 |
punct |
.,shown |
R1757 |
T3065 |
T3066 |
advmod |
Apparently,detected |
R1758 |
T3067 |
T3066 |
punct |
", ",detected |
R1759 |
T3068 |
T3069 |
det |
this,antibody |
R1760 |
T3069 |
T3066 |
nsubj |
antibody,detected |
R1761 |
T3070 |
T3071 |
det |
a,band |
R1762 |
T3071 |
T3066 |
dobj |
band,detected |
R1763 |
T3072 |
T3071 |
amod |
specific,band |
R1764 |
T3073 |
T3071 |
prep |
of,band |
R1765 |
T3074 |
T3073 |
pobj |
Acdp1,of |
R1766 |
T3075 |
T3066 |
prep |
in,detected |
R1767 |
T3076 |
T3077 |
det |
all,lysates |
R1768 |
T3077 |
T3075 |
pobj |
lysates,in |
R1769 |
T3078 |
T3077 |
compound |
cell,lysates |
R1770 |
T3079 |
T3066 |
punct |
.,detected |
R1771 |
T3081 |
T3082 |
prep |
Of,are |
R1772 |
T3083 |
T3081 |
pobj |
note,Of |
R1773 |
T3084 |
T3082 |
punct |
", ",are |
R1774 |
T3085 |
T3082 |
dep |
shown,are |
R1775 |
T3086 |
T3085 |
prep |
in,shown |
R1776 |
T3087 |
T3086 |
pobj |
Fig.,in |
R1777 |
T3088 |
T3087 |
nummod |
6B,Fig. |
R1778 |
T3089 |
T3082 |
nsubj |
signals,are |
R1779 |
T3090 |
T3089 |
prep |
of,signals |
R1780 |
T3091 |
T3092 |
nummod |
10,μg |
R1781 |
T3092 |
T3093 |
compound |
μg,extracts |
R1782 |
T3093 |
T3090 |
pobj |
extracts,of |
R1783 |
T3094 |
T3093 |
prep |
of,extracts |
R1784 |
T3095 |
T3096 |
compound |
HEK293,lysates |
R1785 |
T3096 |
T3094 |
pobj |
lysates,of |
R1786 |
T3097 |
T3096 |
compound |
cell,lysates |
R1787 |
T3098 |
T3093 |
punct |
", ",extracts |
R1788 |
T3099 |
T3100 |
nummod |
100,μg |
R1789 |
T3100 |
T3101 |
compound |
μg,extracts |
R1790 |
T3101 |
T3093 |
appos |
extracts,extracts |
R1791 |
T3102 |
T3101 |
prep |
of,extracts |
R1792 |
T3103 |
T3104 |
nmod |
3T3,lysates |
R1793 |
T3104 |
T3102 |
pobj |
lysates,of |
R1794 |
T3105 |
T3103 |
cc |
and,3T3 |
R1795 |
T3106 |
T3103 |
conj |
PC12,3T3 |
R1796 |
T3107 |
T3104 |
compound |
cell,lysates |
R1797 |
T3108 |
T3082 |
punct |
.,are |
R1798 |
T3110 |
T3111 |
advmod |
Thus,vary |
R1799 |
T3112 |
T3111 |
punct |
", ",vary |
R1800 |
T3113 |
T3114 |
det |
the,levels |
R1801 |
T3114 |
T3111 |
nsubj |
levels,vary |
R1802 |
T3115 |
T3114 |
compound |
expression,levels |
R1803 |
T3116 |
T3114 |
prep |
of,levels |
R1804 |
T3117 |
T3116 |
pobj |
Acdp1,of |
R1805 |
T3118 |
T3114 |
prep |
in,levels |
R1806 |
T3119 |
T3120 |
det |
these,types |
R1807 |
T3120 |
T3118 |
pobj |
types,in |
R1808 |
T3121 |
T3120 |
compound |
cell,types |
R1809 |
T3122 |
T3123 |
det |
a,lot |
R1810 |
T3123 |
T3111 |
dobj |
lot,vary |
R1811 |
T3124 |
T3111 |
punct |
", ",vary |
R1812 |
T3125 |
T3111 |
prep |
with,vary |
R1813 |
T3126 |
T3127 |
det |
the,expression |
R1814 |
T3127 |
T3125 |
pobj |
expression,with |
R1815 |
T3128 |
T3127 |
amod |
highest,expression |
R1816 |
T3129 |
T3127 |
prep |
in,expression |
R1817 |
T3130 |
T3131 |
compound |
HEK293,cells |
R1818 |
T3131 |
T3129 |
pobj |
cells,in |
R1819 |
T3132 |
T3111 |
punct |
.,vary |
R1820 |
T3134 |
T3135 |
advmod |
Nevertheless,support |
R1821 |
T3136 |
T3135 |
punct |
", ",support |
R1822 |
T3137 |
T3138 |
det |
these,results |
R1823 |
T3138 |
T3135 |
nsubj |
results,support |
R1824 |
T3139 |
T3138 |
compound |
immunoblot,results |
R1825 |
T3140 |
T3141 |
poss |
our,analysis |
R1826 |
T3141 |
T3135 |
dobj |
analysis,support |
R1827 |
T3142 |
T3141 |
prep |
of,analysis |
R1828 |
T3143 |
T3144 |
compound |
brain,extracts |
R1829 |
T3144 |
T3142 |
pobj |
extracts,of |
R1830 |
T3145 |
T3144 |
compound |
tissue,extracts |
R1831 |
T3146 |
T3147 |
mark |
that,recognizes |
R1832 |
T3147 |
T3141 |
acl |
recognizes,analysis |
R1833 |
T3148 |
T3149 |
det |
the,antibody |
R1834 |
T3149 |
T3147 |
nsubj |
antibody,recognizes |
R1835 |
T3150 |
T3149 |
prep |
against,antibody |
R1836 |
T3151 |
T3152 |
compound |
Acdp1,terminus |
R1837 |
T3152 |
T3150 |
pobj |
terminus,against |
R1838 |
T3153 |
T3152 |
compound |
C,terminus |
R1839 |
T3154 |
T3152 |
punct |
-,terminus |
R1840 |
T3155 |
T3147 |
advmod |
specifically,recognizes |
R1841 |
T3156 |
T3147 |
dobj |
Acdp,recognizes |
R1842 |
T3157 |
T3156 |
punct |
-,Acdp |
R1843 |
T3158 |
T3156 |
nummod |
1,Acdp |
R1844 |
T3159 |
T3135 |
punct |
.,support |