Id |
Subject |
Object |
Predicate |
Lexical cue |
T6606 |
3306-3307 |
. |
denotes |
. |
T6605 |
3305-3306 |
-RRB- |
denotes |
) |
T6604 |
3299-3302 |
CD |
denotes |
4.5 |
T6603 |
3303-3305 |
NNS |
denotes |
μm |
T6602 |
3298-3299 |
-LRB- |
denotes |
( |
T6601 |
3286-3292 |
JJ |
denotes |
middle |
T6600 |
3293-3297 |
NN |
denotes |
plan |
T6599 |
3282-3285 |
DT |
denotes |
the |
T6598 |
3279-3281 |
IN |
denotes |
to |
T6597 |
3277-3278 |
-RRB- |
denotes |
) |
T6596 |
3273-3274 |
CD |
denotes |
0 |
T6595 |
3275-3277 |
NNS |
denotes |
μm |
T6594 |
3272-3273 |
-LRB- |
denotes |
( |
T6593 |
3265-3271 |
NN |
denotes |
neuron |
T6592 |
3261-3264 |
DT |
denotes |
the |
T6591 |
3258-3260 |
IN |
denotes |
of |
T6590 |
3250-3257 |
NN |
denotes |
surface |
T6589 |
3246-3249 |
DT |
denotes |
the |
T6588 |
3241-3245 |
IN |
denotes |
from |
T6587 |
3239-3241 |
, |
denotes |
, |
T6586 |
3237-3239 |
NNS |
denotes |
μm |
T6585 |
3233-3236 |
CD |
denotes |
0.5 |
T6584 |
3214-3221 |
NN |
denotes |
imaging |
T6583 |
3222-3229 |
NN |
denotes |
section |
T6582 |
3209-3213 |
DT |
denotes |
each |
T6581 |
3206-3208 |
IN |
denotes |
of |
T6580 |
3230-3232 |
VBZ |
denotes |
is |
T6579 |
3201-3205 |
NN |
denotes |
step |
T6578 |
3197-3200 |
DT |
denotes |
The |
T6577 |
3196-3307 |
sentence |
denotes |
The step of each imaging section is 0.5 μm, from the surface of the neuron (0 μm) to the middle plan (4.5 μm). |
T6576 |
3195-3196 |
. |
denotes |
. |
T6575 |
3176-3186 |
JJ |
denotes |
anti-Acdp1 |
T6574 |
3187-3195 |
NN |
denotes |
antibody |
T6573 |
3173-3175 |
DT |
denotes |
an |
T6572 |
3168-3172 |
IN |
denotes |
with |
T6571 |
3160-3167 |
VBN |
denotes |
stained |
T6570 |
3144-3152 |
VBN |
denotes |
cultured |
T6569 |
3153-3159 |
NN |
denotes |
neuron |
T6568 |
3142-3143 |
DT |
denotes |
a |
T6567 |
3137-3141 |
IN |
denotes |
from |
T6566 |
3130-3136 |
NNS |
denotes |
images |
T6565 |
3121-3129 |
JJ |
denotes |
confocal |
T6564 |
3118-3120 |
IN |
denotes |
of |
T6563 |
3111-3117 |
NN |
denotes |
series |
T6562 |
3109-3110 |
DT |
denotes |
A |
T6561 |
3108-3196 |
sentence |
denotes |
A series of confocal images from a cultured neuron stained with an anti-Acdp1 antibody. |
T6560 |
3107-3108 |
. |
denotes |
. |
T6559 |
3100-3107 |
NNS |
denotes |
neurons |
T6558 |
3088-3099 |
NN |
denotes |
hippocampus |
T6557 |
3085-3087 |
IN |
denotes |
in |
T6556 |
3079-3084 |
NN |
denotes |
Acdp1 |
T6555 |
3076-3078 |
IN |
denotes |
of |
T6554 |
3063-3075 |
NN |
denotes |
localization |
T6553 |
3051-3062 |
JJ |
denotes |
Subcellular |
T6464 |
2776-2784 |
JJ |
denotes |
terminal |
T6463 |
2774-2775 |
NN |
denotes |
C |
T6462 |
2770-2773 |
CC |
denotes |
and |
T6461 |
2785-2793 |
NNS |
denotes |
peptides |
T6460 |
2760-2761 |
HYPH |
denotes |
- |
T6459 |
2761-2769 |
JJ |
denotes |
terminal |
T6458 |
2759-2760 |
NN |
denotes |
N |
T6457 |
2756-2758 |
IN |
denotes |
by |
T6456 |
2746-2755 |
VBN |
denotes |
generated |
T6455 |
2729-2734 |
NN |
denotes |
Acdp1 |
T6454 |
2735-2745 |
NNS |
denotes |
antibodies |
T6453 |
2725-2728 |
DT |
denotes |
the |
T6452 |
2720-2724 |
IN |
denotes |
with |
T6451 |
2711-2713 |
, |
denotes |
, |
T6450 |
2710-2711 |
CD |
denotes |
3 |
T6449 |
2706-2709 |
CC |
denotes |
and |
T6448 |
2713-2719 |
VBN |
denotes |
probed |
T6447 |
2704-2705 |
CD |
denotes |
2 |
T6446 |
2699-2703 |
NN |
denotes |
Lane |
T6445 |
2698-2808 |
sentence |
denotes |
Lane 2 and 3, probed with the Acdp1 antibodies generated by N-terminal and C-terminal peptides, respectively. |
T6444 |
2697-2698 |
. |
denotes |
. |
T6443 |
2696-2697 |
-RRB- |
denotes |
) |
T6442 |
2691-2693 |
CD |
denotes |
80 |
T6441 |
2694-2696 |
NN |
denotes |
kD |
T6440 |
2690-2691 |
-LRB- |
denotes |
( |
T6439 |
2684-2689 |
NN |
denotes |
Acdp3 |
T6438 |
2680-2683 |
CC |
denotes |
and |
T6437 |
2678-2679 |
-RRB- |
denotes |
) |
T6436 |
2673-2675 |
CD |
denotes |
90 |
T6435 |
2676-2678 |
NN |
denotes |
kD |
T6434 |
2672-2673 |
-LRB- |
denotes |
( |
T6433 |
2666-2671 |
NN |
denotes |
Acdp4 |
T6432 |
2664-2666 |
, |
denotes |
, |
T6431 |
2663-2664 |
-RRB- |
denotes |
) |
T6430 |
2657-2660 |
CD |
denotes |
100 |
T6429 |
2661-2663 |
NN |
denotes |
kD |
T6428 |
2656-2657 |
-LRB- |
denotes |
( |
T6427 |
2650-2655 |
NN |
denotes |
Acdp2 |
T6426 |
2648-2650 |
, |
denotes |
, |
T6425 |
2647-2648 |
-RRB- |
denotes |
) |
T6424 |
2641-2644 |
CD |
denotes |
115 |
T6423 |
2645-2647 |
NN |
denotes |
kD |
T6422 |
2640-2641 |
-LRB- |
denotes |
( |
T6421 |
2634-2639 |
NN |
denotes |
Acdp1 |
T6420 |
2631-2633 |
IN |
denotes |
to |
T6419 |
2617-2630 |
VBG |
denotes |
corresponding |
T6418 |
2607-2611 |
DT |
denotes |
each |
T6417 |
2605-2607 |
, |
denotes |
, |
T6416 |
2599-2605 |
NN |
denotes |
bottom |
T6415 |
2596-2598 |
IN |
denotes |
to |
T6414 |
2592-2595 |
NN |
denotes |
top |
T6413 |
2612-2616 |
NN |
denotes |
band |
T6412 |
2587-2591 |
IN |
denotes |
From |
T6411 |
2586-2698 |
sentence |
denotes |
From top to bottom, each band corresponding to Acdp1 (115 kD), Acdp2 (100 kD), Acdp4 (90 kD) and Acdp3 (80 kD). |
T6410 |
2585-2586 |
. |
denotes |
. |
T6409 |
2578-2585 |
NN |
denotes |
peptide |
T6408 |
2568-2577 |
JJ |
denotes |
conserved |
T6407 |
2565-2567 |
IN |
denotes |
by |
T6406 |
2555-2564 |
VBN |
denotes |
generated |
T6405 |
2546-2554 |
NN |
denotes |
antibody |
T6404 |
2541-2545 |
IN |
denotes |
with |
T6403 |
2532-2534 |
, |
denotes |
, |
T6402 |
2531-2532 |
CD |
denotes |
1 |
T6401 |
2534-2540 |
VBN |
denotes |
probed |
T6400 |
2526-2530 |
NN |
denotes |
Lane |
T6399 |
2525-2586 |
sentence |
denotes |
Lane 1, probed with antibody generated by conserved peptide. |
T6398 |
2524-2525 |
. |
denotes |
. |
T6397 |
2523-2524 |
-RRB- |
denotes |
) |
T6396 |
2517-2523 |
NN |
denotes |
PIERCE |
T6395 |
2516-2517 |
-LRB- |
denotes |
( |
T6394 |
2514-2515 |
-RRB- |
denotes |
) |
T6393 |
2511-2514 |
NN |
denotes |
ECL |
T6392 |
2510-2511 |
-LRB- |
denotes |
( |
T6391 |
2493-2502 |
NN |
denotes |
detection |
T6390 |
2484-2492 |
NN |
denotes |
blotting |
T6389 |
2476-2483 |
NNP |
denotes |
Western |
T6388 |
2503-2509 |
NN |
denotes |
system |
T6387 |
2474-2475 |
DT |
denotes |
a |
T6386 |
2468-2473 |
VBG |
denotes |
using |
T6385 |
2464-2467 |
RP |
denotes |
out |
T6384 |
2451-2455 |
VBD |
denotes |
were |
T6383 |
2456-2463 |
VBN |
denotes |
carried |
T6382 |
2436-2450 |
NN |
denotes |
Immunoblotting |
T6381 |
2435-2525 |
sentence |
denotes |
Immunoblotting were carried out using a Western blotting detection system (ECL) (PIERCE). |
T6380 |
2434-2435 |
. |
denotes |
. |
T6379 |
2426-2434 |
NNS |
denotes |
extracts |
T6378 |
2419-2425 |
NN |
denotes |
tissue |
T6377 |
2413-2418 |
NN |
denotes |
brain |
T6376 |
2410-2412 |
IN |
denotes |
in |
T6375 |
2401-2409 |
NN |
denotes |
proteins |
T6374 |
2396-2400 |
NN |
denotes |
Acdp |
T6373 |
2393-2395 |
IN |
denotes |
of |
T6372 |
2373-2383 |
NN |
denotes |
Immunoblot |
T6371 |
2371-2373 |
: |
denotes |
: |
T6370 |
2369-2371 |
CD |
denotes |
6A |
T6369 |
2384-2392 |
NN |
denotes |
analysis |
T6368 |
2364-2368 |
NN |
denotes |
Fig. |
T6528 |
3039-3040 |
. |
denotes |
. |
T6527 |
3027-3031 |
NN |
denotes |
cell |
T6526 |
3022-3026 |
NN |
denotes |
PC12 |
T6525 |
3018-3021 |
CC |
denotes |
and |
T6524 |
3032-3039 |
NNS |
denotes |
lysates |
T6523 |
3014-3017 |
NN |
denotes |
3T3 |
T6522 |
3011-3013 |
IN |
denotes |
of |
T6521 |
3004-3007 |
CD |
denotes |
100 |
T6520 |
3002-3004 |
, |
denotes |
, |
T6519 |
3001-3002 |
CD |
denotes |
3 |
T6518 |
2997-3000 |
CC |
denotes |
and |
T6517 |
3008-3010 |
NNS |
denotes |
μg |
T6516 |
2995-2996 |
CD |
denotes |
2 |
T6515 |
2990-2994 |
NN |
denotes |
Lane |
T6514 |
2989-3040 |
sentence |
denotes |
Lane 2 and 3, 100 μg of 3T3 and PC12 cell lysates. |
T6513 |
2988-2989 |
. |
denotes |
. |
T6512 |
2976-2980 |
NN |
denotes |
cell |
T6511 |
2981-2988 |
NNS |
denotes |
lysates |
T6510 |
2969-2975 |
NN |
denotes |
HEK293 |
T6509 |
2966-2968 |
IN |
denotes |
of |
T6508 |
2960-2962 |
CD |
denotes |
10 |
T6507 |
2958-2960 |
, |
denotes |
, |
T6506 |
2957-2958 |
CD |
denotes |
1 |
T6505 |
2963-2965 |
NNS |
denotes |
μg |
T6504 |
2952-2956 |
NN |
denotes |
Lane |
T6503 |
2951-2989 |
sentence |
denotes |
Lane 1, 10 μg of HEK293 cell lysates. |
T6502 |
2950-2951 |
. |
denotes |
. |
T6501 |
2949-2950 |
CD |
denotes |
1 |
T6500 |
2948-2949 |
HYPH |
denotes |
- |
T6499 |
2944-2948 |
NN |
denotes |
Acdp |
T6498 |
2941-2943 |
IN |
denotes |
of |
T6497 |
2931-2932 |
HYPH |
denotes |
- |
T6496 |
2930-2931 |
NN |
denotes |
C |
T6495 |
2932-2940 |
NN |
denotes |
terminus |
T6494 |
2926-2929 |
DT |
denotes |
the |
T6493 |
2918-2925 |
IN |
denotes |
against |
T6492 |
2909-2917 |
NN |
denotes |
antibody |
T6491 |
2905-2908 |
DT |
denotes |
the |
T6490 |
2900-2904 |
IN |
denotes |
with |
T6489 |
2888-2892 |
VBD |
denotes |
were |
T6488 |
2893-2899 |
VBN |
denotes |
probed |
T6487 |
2882-2887 |
NNS |
denotes |
blots |
T6486 |
2878-2881 |
DT |
denotes |
The |
T6485 |
2877-2951 |
sentence |
denotes |
The blots were probed with the antibody against the C-terminus of Acdp-1. |
T6484 |
2876-2877 |
. |
denotes |
. |
T6483 |
2866-2870 |
NN |
denotes |
PC12 |
T6482 |
2862-2865 |
CC |
denotes |
and |
T6481 |
2858-2861 |
NN |
denotes |
3T3 |
T6480 |
2856-2858 |
, |
denotes |
, |
T6479 |
2871-2876 |
NNS |
denotes |
cells |
T6478 |
2850-2856 |
NN |
denotes |
HEK293 |
T6477 |
2847-2849 |
IN |
denotes |
in |
T6476 |
2841-2846 |
NN |
denotes |
Acdp1 |
T6475 |
2838-2840 |
IN |
denotes |
of |
T6474 |
2818-2828 |
NN |
denotes |
Immunoblot |
T6473 |
2816-2818 |
: |
denotes |
: |
T6472 |
2814-2816 |
CD |
denotes |
6B |
T6471 |
2829-2837 |
NN |
denotes |
analysis |
T6470 |
2809-2813 |
NN |
denotes |
Fig. |
T6469 |
2808-2877 |
sentence |
denotes |
Fig. 6B: Immunoblot analysis of Acdp1 in HEK293, 3T3 and PC12 cells. |
T6468 |
2807-2808 |
. |
denotes |
. |
T6467 |
2795-2807 |
RB |
denotes |
respectively |
T6466 |
2793-2795 |
, |
denotes |
, |
T6465 |
2775-2776 |
HYPH |
denotes |
- |
T3199 |
1761-1763 |
PRP |
denotes |
we |
T3198 |
1759-1761 |
, |
denotes |
, |
T3197 |
1754-1759 |
NN |
denotes |
brain |
T3196 |
1750-1753 |
DT |
denotes |
the |
T3195 |
1747-1749 |
IN |
denotes |
in |
T3194 |
1741-1746 |
NN |
denotes |
Acdp1 |
T3193 |
1738-1740 |
IN |
denotes |
of |
T3192 |
1727-1737 |
NN |
denotes |
expression |
T3191 |
1717-1726 |
JJ |
denotes |
exclusive |
T3190 |
1710-1716 |
RB |
denotes |
almost |
T3189 |
1764-1772 |
VBD |
denotes |
examined |
T3188 |
1696-1700 |
NN |
denotes |
blot |
T3187 |
1687-1695 |
NNP |
denotes |
Northern |
T3186 |
1701-1709 |
VBD |
denotes |
revealed |
T3185 |
1681-1686 |
IN |
denotes |
Since |
T3184 |
1680-1853 |
sentence |
denotes |
Since Northern blot revealed almost exclusive expression of Acdp1 in the brain, we examined its subcellular localization in hippocampus neurons isolated from mouse embryos. |
T3183 |
1679-1680 |
. |
denotes |
. |
T3182 |
1674-1679 |
NNS |
denotes |
cells |
T3181 |
1667-1673 |
IN |
denotes |
within |
T3180 |
1665-1666 |
CD |
denotes |
1 |
T3179 |
1664-1665 |
HYPH |
denotes |
- |
T3178 |
1660-1664 |
NN |
denotes |
Acdp |
T3177 |
1651-1659 |
VB |
denotes |
localize |
T3176 |
1648-1650 |
TO |
denotes |
to |
T3175 |
1645-1647 |
PRP |
denotes |
it |
T3174 |
1639-1644 |
VBG |
denotes |
using |
T3173 |
1636-1638 |
IN |
denotes |
of |
T3172 |
1624-1635 |
NN |
denotes |
possibility |
T3171 |
1620-1623 |
DT |
denotes |
the |
T3170 |
1592-1593 |
HYPH |
denotes |
- |
T3169 |
1593-1601 |
NN |
denotes |
terminus |
T3168 |
1591-1592 |
NN |
denotes |
C |
T3167 |
1585-1590 |
NN |
denotes |
Acdp1 |
T3166 |
1602-1610 |
NN |
denotes |
antibody |
T3165 |
1581-1584 |
DT |
denotes |
the |
T3164 |
1578-1580 |
IN |
denotes |
of |
T3163 |
1611-1619 |
VBZ |
denotes |
suggests |
T3162 |
1566-1577 |
NN |
denotes |
specificity |
T3161 |
1562-1565 |
DT |
denotes |
The |
T3160 |
1561-1680 |
sentence |
denotes |
The specificity of the Acdp1 C-terminus antibody suggests the possibility of using it to localize Acdp-1 within cells. |
T3159 |
1560-1561 |
. |
denotes |
. |
T3158 |
1559-1560 |
CD |
denotes |
1 |
T3157 |
1558-1559 |
HYPH |
denotes |
- |
T3156 |
1554-1558 |
NN |
denotes |
Acdp |
T3155 |
1530-1542 |
RB |
denotes |
specifically |
T3154 |
1520-1521 |
HYPH |
denotes |
- |
T3153 |
1519-1520 |
NN |
denotes |
C |
T3152 |
1521-1529 |
NN |
denotes |
terminus |
T3151 |
1513-1518 |
NN |
denotes |
Acdp1 |
T3150 |
1505-1512 |
IN |
denotes |
against |
T3149 |
1496-1504 |
NN |
denotes |
antibody |
T3148 |
1492-1495 |
DT |
denotes |
the |
T3147 |
1543-1553 |
VBZ |
denotes |
recognizes |
T3146 |
1487-1491 |
IN |
denotes |
that |
T3145 |
1471-1477 |
NN |
denotes |
tissue |
T3144 |
1478-1486 |
NNS |
denotes |
extracts |
T3143 |
1465-1470 |
NN |
denotes |
brain |
T3142 |
1462-1464 |
IN |
denotes |
of |
T3141 |
1453-1461 |
NN |
denotes |
analysis |
T3140 |
1449-1452 |
PRP$ |
denotes |
our |
T3139 |
1422-1432 |
NN |
denotes |
immunoblot |
T3138 |
1433-1440 |
NNS |
denotes |
results |
T3137 |
1416-1421 |
DT |
denotes |
these |
T3136 |
1414-1416 |
, |
denotes |
, |
T3135 |
1441-1448 |
VBP |
denotes |
support |
T3134 |
1402-1414 |
RB |
denotes |
Nevertheless |
T3133 |
1401-1561 |
sentence |
denotes |
Nevertheless, these immunoblot results support our analysis of brain tissue extracts that the antibody against Acdp1 C-terminus specifically recognizes Acdp-1. |
T3132 |
1400-1401 |
. |
denotes |
. |
T3131 |
1395-1400 |
NNS |
denotes |
cells |
T3130 |
1388-1394 |
NN |
denotes |
HEK293 |
T3129 |
1385-1387 |
IN |
denotes |
in |
T3128 |
1366-1373 |
JJS |
denotes |
highest |
T3127 |
1374-1384 |
NN |
denotes |
expression |
T3126 |
1362-1365 |
DT |
denotes |
the |
T3125 |
1357-1361 |
IN |
denotes |
with |
T3124 |
1355-1357 |
, |
denotes |
, |
T3123 |
1352-1355 |
NN |
denotes |
lot |
T3122 |
1350-1351 |
DT |
denotes |
a |
T3121 |
1334-1338 |
NN |
denotes |
cell |
T3120 |
1339-1344 |
NNS |
denotes |
types |
T3119 |
1328-1333 |
DT |
denotes |
these |
T3118 |
1325-1327 |
IN |
denotes |
in |
T3117 |
1319-1324 |
NN |
denotes |
Acdp1 |
T3116 |
1316-1318 |
IN |
denotes |
of |
T3115 |
1298-1308 |
NN |
denotes |
expression |
T3114 |
1309-1315 |
NNS |
denotes |
levels |
T3113 |
1294-1297 |
DT |
denotes |
the |
T3112 |
1292-1294 |
, |
denotes |
, |
T3111 |
1345-1349 |
VBP |
denotes |
vary |
T3110 |
1288-1292 |
RB |
denotes |
Thus |
T3109 |
1287-1401 |
sentence |
denotes |
Thus, the expression levels of Acdp1 in these cell types vary a lot, with the highest expression in HEK293 cells. |
T3108 |
1286-1287 |
. |
denotes |
. |
T3107 |
1274-1278 |
NN |
denotes |
cell |
T3106 |
1269-1273 |
NN |
denotes |
PC12 |
T3105 |
1265-1268 |
CC |
denotes |
and |
T3104 |
1279-1286 |
NNS |
denotes |
lysates |
T3103 |
1261-1264 |
NN |
denotes |
3T3 |
T3102 |
1258-1260 |
IN |
denotes |
of |
T3101 |
1249-1257 |
NNS |
denotes |
extracts |
T3100 |
1246-1248 |
NN |
denotes |
μg |
T3099 |
1242-1245 |
CD |
denotes |
100 |
T3098 |
1240-1242 |
, |
denotes |
, |
T3097 |
1228-1232 |
NN |
denotes |
cell |
T3096 |
1233-1240 |
NNS |
denotes |
lysates |
T3095 |
1221-1227 |
NN |
denotes |
HEK293 |
T3094 |
1218-1220 |
IN |
denotes |
of |
T3093 |
1209-1217 |
NNS |
denotes |
extracts |
T3092 |
1206-1208 |
NN |
denotes |
μg |
T3091 |
1203-1205 |
CD |
denotes |
10 |
T3090 |
1200-1202 |
IN |
denotes |
of |
T3089 |
1192-1199 |
NNS |
denotes |
signals |
T3088 |
1185-1187 |
CD |
denotes |
6B |
T3087 |
1180-1184 |
NN |
denotes |
Fig. |
T3086 |
1177-1179 |
IN |
denotes |
in |
T3085 |
1171-1176 |
VBN |
denotes |
shown |
T3084 |
1169-1171 |
, |
denotes |
, |
T3083 |
1165-1169 |
NN |
denotes |
note |
T3082 |
1188-1191 |
VBP |
denotes |
are |
T3081 |
1162-1164 |
IN |
denotes |
Of |
T3080 |
1161-1287 |
sentence |
denotes |
Of note, shown in Fig. 6B are signals of 10 μg extracts of HEK293 cell lysates, 100 μg extracts of 3T3 and PC12 cell lysates. |
T3079 |
1160-1161 |
. |
denotes |
. |
T3078 |
1148-1152 |
NN |
denotes |
cell |
T3077 |
1153-1160 |
NNS |
denotes |
lysates |
T3076 |
1144-1147 |
DT |
denotes |
all |
T3075 |
1141-1143 |
IN |
denotes |
in |
T3074 |
1135-1140 |
NN |
denotes |
Acdp1 |
T3073 |
1132-1134 |
IN |
denotes |
of |
T3072 |
1118-1126 |
JJ |
denotes |
specific |
T3071 |
1127-1131 |
NN |
denotes |
band |
T3070 |
1116-1117 |
DT |
denotes |
a |
T3069 |
1098-1106 |
NN |
denotes |
antibody |
T3068 |
1093-1097 |
DT |
denotes |
this |
T3067 |
1091-1093 |
, |
denotes |
, |
T3066 |
1107-1115 |
VBD |
denotes |
detected |
T3065 |
1081-1091 |
RB |
denotes |
Apparently |
T3064 |
1080-1161 |
sentence |
denotes |
Apparently, this antibody detected a specific band of Acdp1 in all cell lysates. |
T3063 |
1079-1080 |
. |
denotes |
. |
T3062 |
1077-1079 |
CD |
denotes |
6B |
T3061 |
1072-1076 |
NN |
denotes |
Fig. |
T3060 |
1069-1071 |
IN |
denotes |
in |
T3059 |
1059-1062 |
VBP |
denotes |
are |
T3058 |
1063-1068 |
VBN |
denotes |
shown |
T3057 |
1051-1058 |
NNS |
denotes |
results |
T3056 |
1047-1050 |
DT |
denotes |
The |
T3055 |
1046-1080 |
sentence |
denotes |
The results are shown in Fig. 6B. |
T3054 |
1045-1046 |
. |
denotes |
. |
T3053 |
1035-1039 |
NN |
denotes |
PC12 |
T3052 |
1031-1034 |
CC |
denotes |
and |
T3051 |
1027-1030 |
NN |
denotes |
3T3 |
T3050 |
1025-1027 |
, |
denotes |
, |
T3049 |
1040-1045 |
NNS |
denotes |
cells |
T3048 |
1019-1025 |
NN |
denotes |
HEK293 |
T3047 |
1016-1018 |
IN |
denotes |
of |
T3046 |
1007-1015 |
NNS |
denotes |
extracts |
T3045 |
995-997 |
PRP |
denotes |
we |
T3044 |
993-995 |
, |
denotes |
, |
T3043 |
984-985 |
HYPH |
denotes |
- |
T3042 |
983-984 |
NN |
denotes |
C |
T3041 |
977-982 |
NN |
denotes |
Acdp1 |
T3040 |
985-993 |
NN |
denotes |
terminus |
T3039 |
973-976 |
DT |
denotes |
the |
T3038 |
965-972 |
IN |
denotes |
against |
T3037 |
956-964 |
NN |
denotes |
antibody |
T3036 |
952-955 |
DT |
denotes |
the |
T3035 |
949-951 |
IN |
denotes |
of |
T3034 |
937-948 |
NN |
denotes |
specificity |
T3033 |
933-936 |
DT |
denotes |
the |
T3032 |
998-1006 |
VBD |
denotes |
analyzed |
T3031 |
915-922 |
RB |
denotes |
further |
T3030 |
923-932 |
VB |
denotes |
determine |
T3029 |
912-914 |
TO |
denotes |
To |
T3028 |
911-1046 |
sentence |
denotes |
To further determine the specificity of the antibody against the Acdp1 C-terminus, we analyzed extracts of HEK293, 3T3 and PC12 cells. |
T3027 |
910-911 |
. |
denotes |
. |
T3026 |
909-910 |
-RRB- |
denotes |
) |
T3025 |
908-909 |
CD |
denotes |
1 |
T3024 |
901-903 |
, |
denotes |
, |
T3023 |
899-901 |
CD |
denotes |
6A |
T3022 |
894-898 |
NN |
denotes |
Fig. |
T3021 |
903-907 |
NN |
denotes |
lane |
T3020 |
893-894 |
-LRB- |
denotes |
( |
T3019 |
879-883 |
NN |
denotes |
Acdp |
T3018 |
884-892 |
NN |
denotes |
proteins |
T3017 |
875-878 |
DT |
denotes |
all |
T3016 |
849-857 |
NN |
denotes |
sequence |
T3015 |
839-848 |
VBN |
denotes |
conserved |
T3014 |
858-865 |
NN |
denotes |
peptide |
T3013 |
835-838 |
DT |
denotes |
the |
T3012 |
832-834 |
IN |
denotes |
by |
T3011 |
823-831 |
VBN |
denotes |
produced |
T3010 |
814-822 |
NN |
denotes |
antibody |
T3009 |
810-813 |
DT |
denotes |
the |
T3008 |
808-810 |
, |
denotes |
, |
T3007 |
866-874 |
VBD |
denotes |
detected |
T3006 |
800-808 |
VBN |
denotes |
expected |
T3005 |
797-799 |
IN |
denotes |
As |
T3004 |
796-911 |
sentence |
denotes |
As expected, the antibody produced by the conserved sequence peptide detected all Acdp proteins (Fig. 6A, lane 1). |
T3003 |
795-796 |
. |
denotes |
. |
T3002 |
794-795 |
-RRB- |
denotes |
) |
T3001 |
793-794 |
CD |
denotes |
2 |
T3000 |
786-788 |
, |
denotes |
, |
T2999 |
784-786 |
CD |
denotes |
6A |
T2998 |
779-783 |
NN |
denotes |
Fig. |
T2997 |
788-792 |
NN |
denotes |
lane |
T2996 |
778-779 |
-LRB- |
denotes |
( |
T2995 |
771-777 |
JJR |
denotes |
higher |
T2994 |
757-770 |
RB |
denotes |
significantly |
T2993 |
746-752 |
NN |
denotes |
latter |
T2992 |
743-745 |
IN |
denotes |
to |
T2991 |
732-742 |
NN |
denotes |
reactivity |
T2990 |
728-731 |
DT |
denotes |
the |
T2989 |
753-756 |
VBD |
denotes |
was |
T2988 |
719-727 |
IN |
denotes |
although |
T2987 |
717-719 |
, |
denotes |
, |
T2986 |
712-717 |
NN |
denotes |
Acdp1 |
T2985 |
709-711 |
IN |
denotes |
to |
T2984 |
700-708 |
NN |
denotes |
addition |
T2978 |
661-662 |
NN |
denotes |
N |
T2977 |
658-660 |
IN |
denotes |
by |
T2976 |
648-657 |
VBN |
denotes |
generated |
T2975 |
680-690 |
VBD |
denotes |
recognized |
T2974 |
639-647 |
NN |
denotes |
antibody |
T2973 |
635-638 |
DT |
denotes |
The |
T2972 |
634-796 |
sentence |
denotes |
The antibody generated by N-terminal peptide recognized Acdp4 in addition to Acdp1, although the reactivity to latter was significantly higher (Fig. 6A, lane 2). |
T2971 |
633-634 |
. |
denotes |
. |
T2970 |
632-633 |
-RRB- |
denotes |
) |
T2969 |
631-632 |
CD |
denotes |
3 |
T2968 |
626-630 |
NN |
denotes |
lane |
T2967 |
625-626 |
-LRB- |
denotes |
( |
T2966 |
619-624 |
NN |
denotes |
Acdp1 |
T2965 |
597-609 |
RB |
denotes |
specifically |
T2964 |
580-588 |
JJ |
denotes |
terminal |
T2963 |
579-580 |
HYPH |
denotes |
- |
T2962 |
589-596 |
NN |
denotes |
peptide |
T2961 |
578-579 |
NN |
denotes |
C |
T2960 |
575-577 |
IN |
denotes |
by |
T2959 |
566-574 |
VBN |
denotes |
produced |
T2958 |
557-565 |
NN |
denotes |
antibody |
T2957 |
553-556 |
DT |
denotes |
the |
T2956 |
551-553 |
, |
denotes |
, |
T2955 |
549-551 |
CD |
denotes |
6A |
T2954 |
544-548 |
NN |
denotes |
Fig. |
T2953 |
541-543 |
IN |
denotes |
in |
T2952 |
610-618 |
VBD |
denotes |
detected |
T2951 |
535-540 |
VBN |
denotes |
shown |
T2950 |
532-534 |
IN |
denotes |
As |
T2949 |
531-634 |
sentence |
denotes |
As shown in Fig. 6A, the antibody produced by C-terminal peptide specifically detected Acdp1 (lane 3). |
T2948 |
530-531 |
. |
denotes |
. |
T2947 |
522-530 |
NNS |
denotes |
extracts |
T2946 |
515-521 |
NN |
denotes |
tissue |
T2945 |
509-514 |
NN |
denotes |
brain |
T2944 |
503-508 |
NN |
denotes |
mouse |
T2943 |
500-502 |
IN |
denotes |
of |
T2942 |
491-499 |
NN |
denotes |
analysis |
T2941 |
485-486 |
HYPH |
denotes |
- |
T2940 |
486-490 |
NN |
denotes |
blot |
T2939 |
478-485 |
NNP |
denotes |
Western |
T2938 |
465-467 |
PRP |
denotes |
we |
T2937 |
463-465 |
, |
denotes |
, |
T2936 |
453-463 |
NNS |
denotes |
antibodies |
T2935 |
449-452 |
DT |
denotes |
the |
T2934 |
446-448 |
IN |
denotes |
of |
T2933 |
434-445 |
NN |
denotes |
specificity |
T2932 |
430-433 |
DT |
denotes |
the |
T2931 |
468-477 |
VBD |
denotes |
conducted |
T2930 |
425-429 |
VB |
denotes |
test |
T2929 |
422-424 |
TO |
denotes |
To |
T2928 |
421-531 |
sentence |
denotes |
To test the specificity of the antibodies, we conducted Western-blot analysis of mouse brain tissue extracts. |
T2927 |
420-421 |
. |
denotes |
. |
T2926 |
413-420 |
NNS |
denotes |
rabbits |
T2925 |
402-412 |
VBG |
denotes |
immunizing |
T2924 |
399-401 |
IN |
denotes |
by |
T2923 |
385-389 |
VBD |
denotes |
were |
T2922 |
377-384 |
NN |
denotes |
peptide |
T2921 |
372-376 |
DT |
denotes |
each |
T2920 |
368-371 |
IN |
denotes |
for |
T2919 |
390-398 |
VBN |
denotes |
produced |
T2918 |
357-367 |
NNS |
denotes |
antibodies |
T2917 |
346-356 |
JJ |
denotes |
polyclonal |
T2916 |
342-345 |
CC |
denotes |
and |
T2915 |
334-341 |
NNP |
denotes |
Genosys |
T2914 |
328-333 |
NNP |
denotes |
Sigma |
T2913 |
323-327 |
IN |
denotes |
from |
T2912 |
314-322 |
NN |
denotes |
software |
T2911 |
311-313 |
IN |
denotes |
by |
T2910 |
296-300 |
VBD |
denotes |
were |
T2909 |
301-310 |
VBN |
denotes |
predicted |
T2908 |
280-289 |
JJ |
denotes |
antigenic |
T2907 |
290-295 |
NNS |
denotes |
sites |
T2906 |
274-279 |
DT |
denotes |
These |
T2905 |
273-421 |
sentence |
denotes |
These antigenic sites were predicted by software from Sigma Genosys and polyclonal antibodies for each peptide were produced by immunizing rabbits. |
T2904 |
272-273 |
. |
denotes |
. |
T2903 |
271-272 |
-RRB- |
denotes |
) |
T2902 |
258-263 |
NNP |
denotes |
Sigma |
T2901 |
264-271 |
NNP |
denotes |
Genosys |
T2900 |
257-258 |
-LRB- |
denotes |
( |
T2899 |
232-244 |
RB |
denotes |
commercially |
T2898 |
227-231 |
VBD |
denotes |
were |
T2897 |
225-226 |
-RRB- |
denotes |
) |
T2896 |
196-225 |
NN |
denotes |
HNIVDILFVKDLAFVDPDDCTPLLTVTRF |
T2895 |
195-196 |
-LRB- |
denotes |
( |
T2894 |
189-194 |
NN |
denotes |
Acdp1 |
T2893 |
186-188 |
IN |
denotes |
of |
T2892 |
179-185 |
NN |
denotes |
domain |
T2891 |
175-178 |
NN |
denotes |
ACD |
T2890 |
170-174 |
IN |
denotes |
from |
T2889 |
162-169 |
NN |
denotes |
peptide |
T2888 |
152-161 |
JJ |
denotes |
conserved |
T2887 |
150-152 |
, |
denotes |
, |
T2886 |
138-139 |
-RRB- |
denotes |
) |
T2885 |
124-138 |
NN |
denotes |
TQQLTLSPAAVPTR |
T2884 |
123-124 |
-LRB- |
denotes |
( |
T2883 |
121-122 |
HYPH |
denotes |
- |
T2882 |
120-121 |
NN |
denotes |
C |
T2881 |
116-119 |
CC |
denotes |
and |
T2880 |
114-115 |
-RRB- |
denotes |
) |
T2879 |
90-114 |
NN |
denotes |
TSFLLRVYFQPGPPATAAPVPSPT |
T2878 |
89-90 |
-LRB- |
denotes |
( |
T2877 |
87-88 |
HYPH |
denotes |
- |
T2876 |
86-87 |
NN |
denotes |
N |
T2875 |
140-150 |
NNS |
denotes |
terminuses |
T2874 |
80-85 |
NN |
denotes |
Acdp1 |
T2873 |
75-79 |
IN |
denotes |
from |
T2872 |
245-256 |
VBN |
denotes |
synthesized |
T2871 |
66-74 |
NNS |
denotes |
Peptides |
T2870 |
65-273 |
sentence |
denotes |
Peptides from Acdp1 N- (TSFLLRVYFQPGPPATAAPVPSPT) and C- (TQQLTLSPAAVPTR) terminuses, conserved peptide from ACD domain of Acdp1 (HNIVDILFVKDLAFVDPDDCTPLLTVTRF) were commercially synthesized (Sigma Genosys). |
T2869 |
53-65 |
NN |
denotes |
localization |
T2868 |
41-52 |
JJ |
denotes |
subcellular |
T2867 |
37-40 |
CC |
denotes |
and |
T2866 |
29-36 |
NNS |
denotes |
results |
T2865 |
21-28 |
NNP |
denotes |
Western |
T2864 |
19-21 |
, |
denotes |
, |
T2863 |
9-19 |
NN |
denotes |
production |
T2862 |
0-8 |
NN |
denotes |
Antibody |
T3297 |
2352-2353 |
. |
denotes |
. |
T3296 |
2339-2343 |
NN |
denotes |
Acdp |
T3295 |
2344-2352 |
NN |
denotes |
proteins |
T3294 |
2335-2338 |
DT |
denotes |
the |
T3293 |
2328-2334 |
IN |
denotes |
within |
T3292 |
2320-2327 |
NNS |
denotes |
domains |
T3291 |
2306-2319 |
NN |
denotes |
transmembrane |
T3290 |
2303-2305 |
IN |
denotes |
of |
T3289 |
2291-2302 |
NN |
denotes |
observation |
T3288 |
2287-2290 |
DT |
denotes |
the |
T3287 |
2282-2286 |
IN |
denotes |
with |
T3286 |
2271-2281 |
JJ |
denotes |
consistent |
T3285 |
2268-2270 |
VBZ |
denotes |
is |
T3284 |
2262-2267 |
WDT |
denotes |
which |
T3283 |
2260-2262 |
, |
denotes |
, |
T3282 |
2259-2260 |
-RRB- |
denotes |
) |
T3281 |
2258-2259 |
CD |
denotes |
7 |
T3280 |
2253-2257 |
NN |
denotes |
Fig. |
T3279 |
2252-2253 |
-LRB- |
denotes |
( |
T3278 |
2234-2235 |
HYPH |
denotes |
- |
T3277 |
2235-2251 |
NN |
denotes |
immunoreactivity |
T3276 |
2229-2234 |
NN |
denotes |
Acdp1 |
T3275 |
2226-2228 |
IN |
denotes |
of |
T3274 |
2217-2225 |
NN |
denotes |
location |
T3273 |
2208-2216 |
NN |
denotes |
membrane |
T3272 |
2193-2200 |
RB |
denotes |
clearly |
T3271 |
2182-2192 |
NN |
denotes |
micrometer |
T3270 |
2178-2181 |
CD |
denotes |
0.5 |
T3269 |
2175-2177 |
IN |
denotes |
of |
T3268 |
2165-2174 |
NN |
denotes |
thickness |
T3267 |
2161-2164 |
DT |
denotes |
the |
T3266 |
2158-2160 |
IN |
denotes |
at |
T3265 |
2153-2157 |
NN |
denotes |
cell |
T3264 |
2151-2152 |
DT |
denotes |
a |
T3263 |
2148-2150 |
IN |
denotes |
of |
T3262 |
2139-2147 |
NNS |
denotes |
sections |
T3261 |
2136-2138 |
IN |
denotes |
of |
T3260 |
2201-2207 |
VBD |
denotes |
showed |
T3259 |
2129-2135 |
NN |
denotes |
series |
T3258 |
2127-2128 |
DT |
denotes |
A |
T3257 |
2126-2353 |
sentence |
denotes |
A series of sections of a cell at the thickness of 0.5 micrometer clearly showed membrane location of Acdp1-immunoreactivity (Fig. 7), which is consistent with the observation of transmembrane domains within the Acdp proteins. |
T3256 |
2125-2126 |
. |
denotes |
. |
T3255 |
2110-2116 |
NN |
denotes |
plasma |
T3254 |
2117-2125 |
NN |
denotes |
membrane |
T3253 |
2106-2109 |
DT |
denotes |
the |
T3252 |
2103-2105 |
IN |
denotes |
on |
T3251 |
2079-2092 |
RB |
denotes |
predominantly |
T3250 |
2076-2078 |
VBZ |
denotes |
is |
T3249 |
2070-2075 |
NN |
denotes |
Acdp1 |
T3248 |
2093-2102 |
VBN |
denotes |
localized |
T3247 |
2065-2069 |
IN |
denotes |
that |
T3246 |
2056-2064 |
VBD |
denotes |
revealed |
T3245 |
2048-2055 |
NN |
denotes |
imaging |
T3244 |
2039-2047 |
JJ |
denotes |
Confocal |
T3243 |
2038-2126 |
sentence |
denotes |
Confocal imaging revealed that Acdp1 is predominantly localized on the plasma membrane. |
T3242 |
2037-2038 |
. |
denotes |
. |
T3241 |
2020-2028 |
JJ |
denotes |
specific |
T3240 |
2010-2011 |
HYPH |
denotes |
- |
T3239 |
2011-2019 |
NN |
denotes |
terminus |
T3238 |
2009-2010 |
NN |
denotes |
C |
T3237 |
2003-2008 |
NN |
denotes |
Acdp1 |
T3236 |
2029-2037 |
NN |
denotes |
antibody |
T3235 |
1999-2002 |
DT |
denotes |
the |
T3234 |
1989-1992 |
VBD |
denotes |
was |
T3233 |
1993-1998 |
VBG |
denotes |
using |
T3232 |
1974-1988 |
NN |
denotes |
Immunostaining |
T3231 |
1973-2038 |
sentence |
denotes |
Immunostaining was using the Acdp1 C-terminus specific antibody. |
T3230 |
1972-1973 |
. |
denotes |
. |
T3229 |
1966-1972 |
NNS |
denotes |
dishes |
T3228 |
1963-1965 |
IN |
denotes |
in |
T3227 |
1943-1951 |
JJ |
denotes |
cortical |
T3226 |
1952-1962 |
NNS |
denotes |
astrocytes |
T3225 |
1937-1942 |
NN |
denotes |
mouse |
T3224 |
1934-1936 |
IN |
denotes |
of |
T3223 |
1914-1923 |
JJ |
denotes |
confluent |
T3222 |
1924-1933 |
NN |
denotes |
monolayer |
T3221 |
1912-1913 |
DT |
denotes |
a |
T3220 |
1907-1911 |
IN |
denotes |
with |
T3219 |
1900-1906 |
VBN |
denotes |
coated |
T3218 |
1889-1899 |
NNS |
denotes |
coverslips |
T3217 |
1883-1888 |
NN |
denotes |
glass |
T3216 |
1880-1882 |
IN |
denotes |
on |
T3215 |
1866-1870 |
VBD |
denotes |
were |
T3214 |
1871-1879 |
VBN |
denotes |
cultured |
T3213 |
1858-1865 |
NNS |
denotes |
neurons |
T3212 |
1854-1857 |
DT |
denotes |
The |
T3211 |
1853-1973 |
sentence |
denotes |
The neurons were cultured on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes in dishes. |
T3210 |
1852-1853 |
. |
denotes |
. |
T3209 |
1845-1852 |
NNS |
denotes |
embryos |
T3208 |
1839-1844 |
NN |
denotes |
mouse |
T3207 |
1834-1838 |
IN |
denotes |
from |
T3206 |
1825-1833 |
VBN |
denotes |
isolated |
T3205 |
1817-1824 |
NNS |
denotes |
neurons |
T3204 |
1805-1816 |
NN |
denotes |
hippocampus |
T3203 |
1802-1804 |
IN |
denotes |
in |
T3202 |
1777-1788 |
JJ |
denotes |
subcellular |
T3201 |
1789-1801 |
NN |
denotes |
localization |
T3200 |
1773-1776 |
PRP$ |
denotes |
its |
T2983 |
697-699 |
IN |
denotes |
in |
T2982 |
691-696 |
NN |
denotes |
Acdp4 |
T2981 |
663-671 |
JJ |
denotes |
terminal |
T2980 |
662-663 |
HYPH |
denotes |
- |
T2979 |
672-679 |
NN |
denotes |
peptide |
R1572 |
T2862 |
T2863 |
compound |
Antibody,production |
R1573 |
T2864 |
T2863 |
punct |
", ",production |
R1574 |
T2865 |
T2866 |
compound |
Western,results |
R1575 |
T2866 |
T2863 |
conj |
results,production |
R1576 |
T2867 |
T2866 |
cc |
and,results |
R1577 |
T2868 |
T2869 |
amod |
subcellular,localization |
R1578 |
T2869 |
T2866 |
conj |
localization,results |
R1579 |
T2871 |
T2872 |
nsubjpass |
Peptides,synthesized |
R1580 |
T2873 |
T2871 |
prep |
from,Peptides |
R1581 |
T2874 |
T2875 |
nmod |
Acdp1,terminuses |
R1582 |
T2875 |
T2873 |
pobj |
terminuses,from |
R1583 |
T2876 |
T2875 |
nmod |
N,terminuses |
R1584 |
T2877 |
T2876 |
punct |
-,N |
R1585 |
T2878 |
T2876 |
punct |
(,N |
R1586 |
T2879 |
T2876 |
appos |
TSFLLRVYFQPGPPATAAPVPSPT,N |
R1587 |
T2880 |
T2876 |
punct |
),N |
R1588 |
T2881 |
T2876 |
cc |
and,N |
R1589 |
T2882 |
T2876 |
conj |
C,N |
R1590 |
T2883 |
T2882 |
punct |
-,C |
R1591 |
T2884 |
T2882 |
punct |
(,C |
R1592 |
T2885 |
T2882 |
appos |
TQQLTLSPAAVPTR,C |
R1593 |
T2886 |
T2875 |
punct |
),terminuses |
R1594 |
T2887 |
T2871 |
punct |
", ",Peptides |
R1595 |
T2888 |
T2889 |
amod |
conserved,peptide |
R1596 |
T2889 |
T2871 |
appos |
peptide,Peptides |
R1597 |
T2890 |
T2889 |
prep |
from,peptide |
R1598 |
T2891 |
T2892 |
compound |
ACD,domain |
R1599 |
T2892 |
T2890 |
pobj |
domain,from |
R1600 |
T2893 |
T2892 |
prep |
of,domain |
R1601 |
T2894 |
T2893 |
pobj |
Acdp1,of |
R1602 |
T2895 |
T2894 |
punct |
(,Acdp1 |
R1603 |
T2896 |
T2894 |
appos |
HNIVDILFVKDLAFVDPDDCTPLLTVTRF,Acdp1 |
R1604 |
T2897 |
T2872 |
punct |
),synthesized |
R1605 |
T2898 |
T2872 |
auxpass |
were,synthesized |
R1606 |
T2899 |
T2872 |
advmod |
commercially,synthesized |
R1607 |
T2900 |
T2901 |
punct |
(,Genosys |
R1608 |
T2901 |
T2872 |
parataxis |
Genosys,synthesized |
R1609 |
T2902 |
T2901 |
compound |
Sigma,Genosys |
R1610 |
T2903 |
T2901 |
punct |
),Genosys |
R1611 |
T2904 |
T2872 |
punct |
.,synthesized |
R1612 |
T2906 |
T2907 |
det |
These,sites |
R1613 |
T2907 |
T2909 |
nsubjpass |
sites,predicted |
R1614 |
T2908 |
T2907 |
amod |
antigenic,sites |
R1615 |
T2910 |
T2909 |
auxpass |
were,predicted |
R1616 |
T2911 |
T2909 |
prep |
by,predicted |
R1617 |
T2912 |
T2911 |
pobj |
software,by |
R1618 |
T2913 |
T2912 |
prep |
from,software |
R1619 |
T2914 |
T2915 |
compound |
Sigma,Genosys |
R1620 |
T2915 |
T2913 |
pobj |
Genosys,from |
R1621 |
T2916 |
T2909 |
cc |
and,predicted |
R1622 |
T2917 |
T2918 |
amod |
polyclonal,antibodies |
R1623 |
T2918 |
T2919 |
nsubjpass |
antibodies,produced |
R1624 |
T2919 |
T2909 |
conj |
produced,predicted |
R1625 |
T2920 |
T2918 |
prep |
for,antibodies |
R1626 |
T2921 |
T2922 |
det |
each,peptide |
R1627 |
T2922 |
T2920 |
pobj |
peptide,for |
R1628 |
T2923 |
T2919 |
auxpass |
were,produced |
R1629 |
T2924 |
T2919 |
prep |
by,produced |
R1630 |
T2925 |
T2924 |
pcomp |
immunizing,by |
R1631 |
T2926 |
T2925 |
dobj |
rabbits,immunizing |
R1632 |
T2927 |
T2919 |
punct |
.,produced |
R1633 |
T2929 |
T2930 |
aux |
To,test |
R1634 |
T2930 |
T2931 |
advcl |
test,conducted |
R1635 |
T2932 |
T2933 |
det |
the,specificity |
R1636 |
T2933 |
T2930 |
dobj |
specificity,test |
R1637 |
T2934 |
T2933 |
prep |
of,specificity |
R1638 |
T2935 |
T2936 |
det |
the,antibodies |
R1639 |
T2936 |
T2934 |
pobj |
antibodies,of |
R1640 |
T2937 |
T2931 |
punct |
", ",conducted |
R1641 |
T2938 |
T2931 |
nsubj |
we,conducted |
R1642 |
T2939 |
T2940 |
compound |
Western,blot |
R1643 |
T2940 |
T2942 |
compound |
blot,analysis |
R1644 |
T2941 |
T2940 |
punct |
-,blot |
R1645 |
T2942 |
T2931 |
dobj |
analysis,conducted |
R1646 |
T2943 |
T2942 |
prep |
of,analysis |
R1647 |
T2944 |
T2945 |
compound |
mouse,brain |
R1648 |
T2945 |
T2946 |
compound |
brain,tissue |
R1649 |
T2946 |
T2947 |
compound |
tissue,extracts |
R1650 |
T2947 |
T2943 |
pobj |
extracts,of |
R1651 |
T2948 |
T2931 |
punct |
.,conducted |
R1652 |
T2950 |
T2951 |
mark |
As,shown |
R1653 |
T2951 |
T2952 |
advcl |
shown,detected |
R1654 |
T2953 |
T2951 |
prep |
in,shown |
R1655 |
T2954 |
T2953 |
pobj |
Fig.,in |
R1656 |
T2955 |
T2954 |
nummod |
6A,Fig. |
R1657 |
T2956 |
T2952 |
punct |
", ",detected |
R1658 |
T2957 |
T2958 |
det |
the,antibody |
R1659 |
T2958 |
T2952 |
nsubj |
antibody,detected |
R1660 |
T2959 |
T2958 |
acl |
produced,antibody |
R1661 |
T2960 |
T2959 |
prep |
by,produced |
R1662 |
T2961 |
T2962 |
nmod |
C,peptide |
R1663 |
T2962 |
T2960 |
pobj |
peptide,by |
R1664 |
T2963 |
T2961 |
punct |
-,C |
R1665 |
T2964 |
T2961 |
amod |
terminal,C |
R1666 |
T2965 |
T2952 |
advmod |
specifically,detected |
R1667 |
T2966 |
T2952 |
dobj |
Acdp1,detected |
R1668 |
T2967 |
T2968 |
punct |
(,lane |
R1669 |
T2968 |
T2952 |
parataxis |
lane,detected |
R1670 |
T2969 |
T2968 |
nummod |
3,lane |
R1671 |
T2970 |
T2968 |
punct |
),lane |
R1672 |
T2971 |
T2952 |
punct |
.,detected |
R1673 |
T2973 |
T2974 |
det |
The,antibody |
R1674 |
T2974 |
T2975 |
nsubj |
antibody,recognized |
R1675 |
T2976 |
T2974 |
acl |
generated,antibody |
R1676 |
T2977 |
T2976 |
agent |
by,generated |
R1677 |
T2978 |
T2979 |
nmod |
N,peptide |
R1678 |
T2979 |
T2977 |
pobj |
peptide,by |
R1679 |
T2980 |
T2978 |
punct |
-,N |
R1680 |
T2981 |
T2978 |
amod |
terminal,N |
R1681 |
T2982 |
T2975 |
dobj |
Acdp4,recognized |
R1682 |
T2983 |
T2975 |
prep |
in,recognized |
R1683 |
T2984 |
T2983 |
pobj |
addition,in |
R1684 |
T2985 |
T2984 |
prep |
to,addition |
R1685 |
T2986 |
T2985 |
pobj |
Acdp1,to |
R1686 |
T2987 |
T2975 |
punct |
", ",recognized |
R1687 |
T2988 |
T2989 |
mark |
although,was |
R1688 |
T2989 |
T2975 |
advcl |
was,recognized |
R1689 |
T2990 |
T2991 |
det |
the,reactivity |
R1690 |
T2991 |
T2989 |
nsubj |
reactivity,was |
R1691 |
T2992 |
T2991 |
prep |
to,reactivity |
R1692 |
T2993 |
T2992 |
pobj |
latter,to |
R1693 |
T2994 |
T2995 |
advmod |
significantly,higher |
R1694 |
T2995 |
T2989 |
acomp |
higher,was |
R1695 |
T2996 |
T2997 |
punct |
(,lane |
R1696 |
T2997 |
T2975 |
parataxis |
lane,recognized |
R1697 |
T2998 |
T2997 |
dep |
Fig.,lane |
R1698 |
T2999 |
T2998 |
nummod |
6A,Fig. |
R1699 |
T3000 |
T2997 |
punct |
", ",lane |
R1700 |
T3001 |
T2997 |
nummod |
2,lane |
R1701 |
T3002 |
T2997 |
punct |
),lane |
R1702 |
T3003 |
T2975 |
punct |
.,recognized |
R1703 |
T3005 |
T3006 |
mark |
As,expected |
R1704 |
T3006 |
T3007 |
advcl |
expected,detected |
R1705 |
T3008 |
T3007 |
punct |
", ",detected |
R1706 |
T3009 |
T3010 |
det |
the,antibody |
R1707 |
T3010 |
T3007 |
nsubj |
antibody,detected |
R1708 |
T3011 |
T3010 |
acl |
produced,antibody |
R1709 |
T3012 |
T3011 |
agent |
by,produced |
R1710 |
T3013 |
T3014 |
det |
the,peptide |
R1711 |
T3014 |
T3012 |
pobj |
peptide,by |
R1712 |
T3015 |
T3014 |
amod |
conserved,peptide |
R1713 |
T3016 |
T3014 |
compound |
sequence,peptide |
R1714 |
T3017 |
T3018 |
det |
all,proteins |
R1715 |
T3018 |
T3007 |
dobj |
proteins,detected |
R1716 |
T3019 |
T3018 |
compound |
Acdp,proteins |
R1717 |
T3020 |
T3021 |
punct |
(,lane |
R1718 |
T3021 |
T3007 |
parataxis |
lane,detected |
R1719 |
T3022 |
T3021 |
dep |
Fig.,lane |
R1720 |
T3023 |
T3022 |
nummod |
6A,Fig. |
R1721 |
T3024 |
T3021 |
punct |
", ",lane |
R1722 |
T3025 |
T3021 |
nummod |
1,lane |
R1723 |
T3026 |
T3021 |
punct |
),lane |
R1724 |
T3027 |
T3007 |
punct |
.,detected |
R1725 |
T3029 |
T3030 |
aux |
To,determine |
R1726 |
T3030 |
T3032 |
advcl |
determine,analyzed |
R1727 |
T3031 |
T3030 |
advmod |
further,determine |
R1728 |
T3033 |
T3034 |
det |
the,specificity |
R1729 |
T3034 |
T3030 |
dobj |
specificity,determine |
R1730 |
T3035 |
T3034 |
prep |
of,specificity |
R1731 |
T3036 |
T3037 |
det |
the,antibody |
R1732 |
T3037 |
T3035 |
pobj |
antibody,of |
R1733 |
T3038 |
T3034 |
prep |
against,specificity |
R1734 |
T3039 |
T3040 |
det |
the,terminus |
R1735 |
T3040 |
T3038 |
pobj |
terminus,against |
R1736 |
T3041 |
T3040 |
compound |
Acdp1,terminus |
R1737 |
T3042 |
T3040 |
compound |
C,terminus |
R1738 |
T3043 |
T3040 |
punct |
-,terminus |
R1739 |
T3044 |
T3032 |
punct |
", ",analyzed |
R1740 |
T3045 |
T3032 |
nsubj |
we,analyzed |
R1741 |
T3046 |
T3032 |
dobj |
extracts,analyzed |
R1742 |
T3047 |
T3046 |
prep |
of,extracts |
R1743 |
T3048 |
T3049 |
nmod |
HEK293,cells |
R1744 |
T3049 |
T3047 |
pobj |
cells,of |
R1745 |
T3050 |
T3048 |
punct |
", ",HEK293 |
R1746 |
T3051 |
T3048 |
conj |
3T3,HEK293 |
R1747 |
T3052 |
T3051 |
cc |
and,3T3 |
R1748 |
T3053 |
T3051 |
conj |
PC12,3T3 |
R1749 |
T3054 |
T3032 |
punct |
.,analyzed |
R1750 |
T3056 |
T3057 |
det |
The,results |
R1751 |
T3057 |
T3058 |
nsubjpass |
results,shown |
R1752 |
T3059 |
T3058 |
auxpass |
are,shown |
R1753 |
T3060 |
T3058 |
prep |
in,shown |
R1754 |
T3061 |
T3060 |
pobj |
Fig.,in |
R1755 |
T3062 |
T3061 |
nummod |
6B,Fig. |
R1756 |
T3063 |
T3058 |
punct |
.,shown |
R1757 |
T3065 |
T3066 |
advmod |
Apparently,detected |
R1758 |
T3067 |
T3066 |
punct |
", ",detected |
R1759 |
T3068 |
T3069 |
det |
this,antibody |
R1760 |
T3069 |
T3066 |
nsubj |
antibody,detected |
R1761 |
T3070 |
T3071 |
det |
a,band |
R1762 |
T3071 |
T3066 |
dobj |
band,detected |
R1763 |
T3072 |
T3071 |
amod |
specific,band |
R1764 |
T3073 |
T3071 |
prep |
of,band |
R1765 |
T3074 |
T3073 |
pobj |
Acdp1,of |
R1766 |
T3075 |
T3066 |
prep |
in,detected |
R1767 |
T3076 |
T3077 |
det |
all,lysates |
R1768 |
T3077 |
T3075 |
pobj |
lysates,in |
R1769 |
T3078 |
T3077 |
compound |
cell,lysates |
R1770 |
T3079 |
T3066 |
punct |
.,detected |
R1771 |
T3081 |
T3082 |
prep |
Of,are |
R1772 |
T3083 |
T3081 |
pobj |
note,Of |
R1773 |
T3084 |
T3082 |
punct |
", ",are |
R1774 |
T3085 |
T3082 |
dep |
shown,are |
R1775 |
T3086 |
T3085 |
prep |
in,shown |
R1776 |
T3087 |
T3086 |
pobj |
Fig.,in |
R1777 |
T3088 |
T3087 |
nummod |
6B,Fig. |
R1778 |
T3089 |
T3082 |
nsubj |
signals,are |
R1779 |
T3090 |
T3089 |
prep |
of,signals |
R1780 |
T3091 |
T3092 |
nummod |
10,μg |
R1781 |
T3092 |
T3093 |
compound |
μg,extracts |
R1782 |
T3093 |
T3090 |
pobj |
extracts,of |
R1783 |
T3094 |
T3093 |
prep |
of,extracts |
R1784 |
T3095 |
T3096 |
compound |
HEK293,lysates |
R1785 |
T3096 |
T3094 |
pobj |
lysates,of |
R1786 |
T3097 |
T3096 |
compound |
cell,lysates |
R1787 |
T3098 |
T3093 |
punct |
", ",extracts |
R1788 |
T3099 |
T3100 |
nummod |
100,μg |
R1789 |
T3100 |
T3101 |
compound |
μg,extracts |
R1790 |
T3101 |
T3093 |
appos |
extracts,extracts |
R1791 |
T3102 |
T3101 |
prep |
of,extracts |
R1792 |
T3103 |
T3104 |
nmod |
3T3,lysates |
R1793 |
T3104 |
T3102 |
pobj |
lysates,of |
R1794 |
T3105 |
T3103 |
cc |
and,3T3 |
R1795 |
T3106 |
T3103 |
conj |
PC12,3T3 |
R1796 |
T3107 |
T3104 |
compound |
cell,lysates |
R1797 |
T3108 |
T3082 |
punct |
.,are |
R1798 |
T3110 |
T3111 |
advmod |
Thus,vary |
R1799 |
T3112 |
T3111 |
punct |
", ",vary |
R1800 |
T3113 |
T3114 |
det |
the,levels |
R1801 |
T3114 |
T3111 |
nsubj |
levels,vary |
R1802 |
T3115 |
T3114 |
compound |
expression,levels |
R1803 |
T3116 |
T3114 |
prep |
of,levels |
R1804 |
T3117 |
T3116 |
pobj |
Acdp1,of |
R1805 |
T3118 |
T3114 |
prep |
in,levels |
R1806 |
T3119 |
T3120 |
det |
these,types |
R1807 |
T3120 |
T3118 |
pobj |
types,in |
R1808 |
T3121 |
T3120 |
compound |
cell,types |
R1809 |
T3122 |
T3123 |
det |
a,lot |
R1810 |
T3123 |
T3111 |
dobj |
lot,vary |
R1811 |
T3124 |
T3111 |
punct |
", ",vary |
R1812 |
T3125 |
T3111 |
prep |
with,vary |
R1813 |
T3126 |
T3127 |
det |
the,expression |
R1814 |
T3127 |
T3125 |
pobj |
expression,with |
R1815 |
T3128 |
T3127 |
amod |
highest,expression |
R1816 |
T3129 |
T3127 |
prep |
in,expression |
R1817 |
T3130 |
T3131 |
compound |
HEK293,cells |
R1818 |
T3131 |
T3129 |
pobj |
cells,in |
R1819 |
T3132 |
T3111 |
punct |
.,vary |
R1820 |
T3134 |
T3135 |
advmod |
Nevertheless,support |
R1821 |
T3136 |
T3135 |
punct |
", ",support |
R1822 |
T3137 |
T3138 |
det |
these,results |
R1823 |
T3138 |
T3135 |
nsubj |
results,support |
R1824 |
T3139 |
T3138 |
compound |
immunoblot,results |
R1825 |
T3140 |
T3141 |
poss |
our,analysis |
R1826 |
T3141 |
T3135 |
dobj |
analysis,support |
R1827 |
T3142 |
T3141 |
prep |
of,analysis |
R1828 |
T3143 |
T3144 |
compound |
brain,extracts |
R1829 |
T3144 |
T3142 |
pobj |
extracts,of |
R1830 |
T3145 |
T3144 |
compound |
tissue,extracts |
R1831 |
T3146 |
T3147 |
mark |
that,recognizes |
R1832 |
T3147 |
T3141 |
acl |
recognizes,analysis |
R1833 |
T3148 |
T3149 |
det |
the,antibody |
R1834 |
T3149 |
T3147 |
nsubj |
antibody,recognizes |
R1835 |
T3150 |
T3149 |
prep |
against,antibody |
R1836 |
T3151 |
T3152 |
compound |
Acdp1,terminus |
R1837 |
T3152 |
T3150 |
pobj |
terminus,against |
R1838 |
T3153 |
T3152 |
compound |
C,terminus |
R1839 |
T3154 |
T3152 |
punct |
-,terminus |
R1840 |
T3155 |
T3147 |
advmod |
specifically,recognizes |
R1841 |
T3156 |
T3147 |
dobj |
Acdp,recognizes |
R1842 |
T3157 |
T3156 |
punct |
-,Acdp |
R1843 |
T3158 |
T3156 |
nummod |
1,Acdp |
R1844 |
T3159 |
T3135 |
punct |
.,support |
R1845 |
T3161 |
T3162 |
det |
The,specificity |
R1846 |
T3162 |
T3163 |
nsubj |
specificity,suggests |
R1847 |
T3164 |
T3162 |
prep |
of,specificity |
R1848 |
T3165 |
T3166 |
det |
the,antibody |
R1849 |
T3166 |
T3164 |
pobj |
antibody,of |
R1850 |
T3167 |
T3166 |
compound |
Acdp1,antibody |
R1851 |
T3168 |
T3169 |
compound |
C,terminus |
R1852 |
T3169 |
T3166 |
compound |
terminus,antibody |
R1853 |
T3170 |
T3169 |
punct |
-,terminus |
R1854 |
T3171 |
T3172 |
det |
the,possibility |
R1855 |
T3172 |
T3163 |
dobj |
possibility,suggests |
R1856 |
T3173 |
T3172 |
prep |
of,possibility |
R1857 |
T3174 |
T3173 |
pcomp |
using,of |
R1858 |
T3175 |
T3174 |
dobj |
it,using |
R1859 |
T3176 |
T3177 |
aux |
to,localize |
R1860 |
T3177 |
T3174 |
advcl |
localize,using |
R1861 |
T3178 |
T3177 |
dobj |
Acdp,localize |
R1862 |
T3179 |
T3178 |
punct |
-,Acdp |
R1863 |
T3180 |
T3178 |
nummod |
1,Acdp |
R1864 |
T3181 |
T3177 |
prep |
within,localize |
R1865 |
T3182 |
T3181 |
pobj |
cells,within |
R1866 |
T3183 |
T3163 |
punct |
.,suggests |
R1867 |
T3185 |
T3186 |
mark |
Since,revealed |
R1868 |
T3186 |
T3189 |
advcl |
revealed,examined |
R1869 |
T3187 |
T3188 |
compound |
Northern,blot |
R1870 |
T3188 |
T3186 |
nsubj |
blot,revealed |
R1871 |
T3190 |
T3191 |
advmod |
almost,exclusive |
R1872 |
T3191 |
T3192 |
amod |
exclusive,expression |
R1873 |
T3192 |
T3186 |
dobj |
expression,revealed |
R1874 |
T3193 |
T3192 |
prep |
of,expression |
R1875 |
T3194 |
T3193 |
pobj |
Acdp1,of |
R1876 |
T3195 |
T3186 |
prep |
in,revealed |
R1877 |
T3196 |
T3197 |
det |
the,brain |
R1878 |
T3197 |
T3195 |
pobj |
brain,in |
R1879 |
T3198 |
T3189 |
punct |
", ",examined |
R1880 |
T3199 |
T3189 |
nsubj |
we,examined |
R1881 |
T3200 |
T3201 |
poss |
its,localization |
R1882 |
T3201 |
T3189 |
dobj |
localization,examined |
R1883 |
T3202 |
T3201 |
amod |
subcellular,localization |
R1884 |
T3203 |
T3189 |
prep |
in,examined |
R1885 |
T3204 |
T3205 |
compound |
hippocampus,neurons |
R1886 |
T3205 |
T3203 |
pobj |
neurons,in |
R1887 |
T3206 |
T3205 |
acl |
isolated,neurons |
R1888 |
T3207 |
T3206 |
prep |
from,isolated |
R1889 |
T3208 |
T3209 |
compound |
mouse,embryos |
R1890 |
T3209 |
T3207 |
pobj |
embryos,from |
R1891 |
T3210 |
T3189 |
punct |
.,examined |
R1892 |
T3212 |
T3213 |
det |
The,neurons |
R1893 |
T3213 |
T3214 |
nsubjpass |
neurons,cultured |
R1894 |
T3215 |
T3214 |
auxpass |
were,cultured |
R1895 |
T3216 |
T3214 |
prep |
on,cultured |
R1896 |
T3217 |
T3218 |
compound |
glass,coverslips |
R1897 |
T3218 |
T3216 |
pobj |
coverslips,on |
R1898 |
T3219 |
T3218 |
acl |
coated,coverslips |
R1899 |
T3220 |
T3219 |
prep |
with,coated |
R1900 |
T3221 |
T3222 |
det |
a,monolayer |
R1901 |
T3222 |
T3220 |
pobj |
monolayer,with |
R1902 |
T3223 |
T3222 |
amod |
confluent,monolayer |
R1903 |
T3224 |
T3222 |
prep |
of,monolayer |
R1904 |
T3225 |
T3226 |
nmod |
mouse,astrocytes |
R1905 |
T3226 |
T3224 |
pobj |
astrocytes,of |
R1906 |
T3227 |
T3226 |
amod |
cortical,astrocytes |
R1907 |
T3228 |
T3214 |
prep |
in,cultured |
R1908 |
T3229 |
T3228 |
pobj |
dishes,in |
R1909 |
T3230 |
T3214 |
punct |
.,cultured |
R1910 |
T3232 |
T3233 |
nsubj |
Immunostaining,using |
R1911 |
T3234 |
T3233 |
aux |
was,using |
R1912 |
T3235 |
T3236 |
det |
the,antibody |
R1913 |
T3236 |
T3233 |
dobj |
antibody,using |
R1914 |
T3237 |
T3236 |
nmod |
Acdp1,antibody |
R1915 |
T3238 |
T3239 |
compound |
C,terminus |
R1916 |
T3239 |
T3241 |
npadvmod |
terminus,specific |
R1917 |
T3240 |
T3239 |
punct |
-,terminus |
R1918 |
T3241 |
T3236 |
amod |
specific,antibody |
R1919 |
T3242 |
T3233 |
punct |
.,using |
R1920 |
T3244 |
T3245 |
amod |
Confocal,imaging |
R1921 |
T3245 |
T3246 |
nsubj |
imaging,revealed |
R1922 |
T3247 |
T3248 |
mark |
that,localized |
R1923 |
T3248 |
T3246 |
ccomp |
localized,revealed |
R1924 |
T3249 |
T3248 |
nsubjpass |
Acdp1,localized |
R1925 |
T3250 |
T3248 |
auxpass |
is,localized |
R1926 |
T3251 |
T3248 |
advmod |
predominantly,localized |
R1927 |
T3252 |
T3248 |
prep |
on,localized |
R1928 |
T3253 |
T3254 |
det |
the,membrane |
R1929 |
T3254 |
T3252 |
pobj |
membrane,on |
R1930 |
T3255 |
T3254 |
compound |
plasma,membrane |
R1931 |
T3256 |
T3246 |
punct |
.,revealed |
R1932 |
T3258 |
T3259 |
det |
A,series |
R1933 |
T3259 |
T3260 |
nsubj |
series,showed |
R1934 |
T3261 |
T3259 |
prep |
of,series |
R1935 |
T3262 |
T3261 |
pobj |
sections,of |
R1936 |
T3263 |
T3262 |
prep |
of,sections |
R1937 |
T3264 |
T3265 |
det |
a,cell |
R1938 |
T3265 |
T3263 |
pobj |
cell,of |
R1939 |
T3266 |
T3259 |
prep |
at,series |
R1940 |
T3267 |
T3268 |
det |
the,thickness |
R1941 |
T3268 |
T3266 |
pobj |
thickness,at |
R1942 |
T3269 |
T3268 |
prep |
of,thickness |
R1943 |
T3270 |
T3271 |
nummod |
0.5,micrometer |
R1944 |
T3271 |
T3269 |
pobj |
micrometer,of |
R1945 |
T3272 |
T3260 |
advmod |
clearly,showed |
R1946 |
T3273 |
T3274 |
compound |
membrane,location |
R1947 |
T3274 |
T3260 |
dobj |
location,showed |
R1948 |
T3275 |
T3274 |
prep |
of,location |
R1949 |
T3276 |
T3277 |
compound |
Acdp1,immunoreactivity |
R1950 |
T3277 |
T3275 |
pobj |
immunoreactivity,of |
R1951 |
T3278 |
T3277 |
punct |
-,immunoreactivity |
R1952 |
T3279 |
T3280 |
punct |
(,Fig. |
R1953 |
T3280 |
T3277 |
parataxis |
Fig.,immunoreactivity |
R1954 |
T3281 |
T3280 |
nummod |
7,Fig. |
R1955 |
T3282 |
T3280 |
punct |
),Fig. |
R1956 |
T3283 |
T3260 |
punct |
", ",showed |
R1957 |
T3284 |
T3285 |
dep |
which,is |
R1958 |
T3285 |
T3260 |
advcl |
is,showed |
R1959 |
T3286 |
T3285 |
acomp |
consistent,is |
R1960 |
T3287 |
T3286 |
prep |
with,consistent |
R1961 |
T3288 |
T3289 |
det |
the,observation |
R1962 |
T3289 |
T3287 |
pobj |
observation,with |
R1963 |
T3290 |
T3289 |
prep |
of,observation |
R1964 |
T3291 |
T3292 |
compound |
transmembrane,domains |
R1965 |
T3292 |
T3290 |
pobj |
domains,of |
R1966 |
T3293 |
T3289 |
prep |
within,observation |
R1967 |
T3294 |
T3295 |
det |
the,proteins |
R1968 |
T3295 |
T3293 |
pobj |
proteins,within |
R1969 |
T3296 |
T3295 |
compound |
Acdp,proteins |
R1970 |
T3297 |
T3260 |
punct |
.,showed |
R3899 |
T6368 |
T6369 |
dep |
Fig.,analysis |
R3900 |
T6370 |
T6368 |
nummod |
6A,Fig. |
R3901 |
T6371 |
T6369 |
punct |
: ,analysis |
R3902 |
T6372 |
T6369 |
compound |
Immunoblot,analysis |
R3903 |
T6373 |
T6369 |
prep |
of,analysis |
R3904 |
T6374 |
T6375 |
compound |
Acdp,proteins |
R3905 |
T6375 |
T6373 |
pobj |
proteins,of |
R3906 |
T6376 |
T6375 |
prep |
in,proteins |
R3907 |
T6377 |
T6378 |
compound |
brain,tissue |
R3908 |
T6378 |
T6379 |
compound |
tissue,extracts |
R3909 |
T6379 |
T6376 |
pobj |
extracts,in |
R3910 |
T6380 |
T6369 |
punct |
.,analysis |
R3911 |
T6382 |
T6383 |
nsubjpass |
Immunoblotting,carried |
R3912 |
T6384 |
T6383 |
auxpass |
were,carried |
R3913 |
T6385 |
T6383 |
prt |
out,carried |
R3914 |
T6386 |
T6383 |
advcl |
using,carried |
R3915 |
T6387 |
T6388 |
det |
a,system |
R3916 |
T6388 |
T6386 |
dobj |
system,using |
R3917 |
T6389 |
T6390 |
compound |
Western,blotting |
R3918 |
T6390 |
T6388 |
compound |
blotting,system |
R3919 |
T6391 |
T6388 |
compound |
detection,system |
R3920 |
T6392 |
T6393 |
punct |
(,ECL |
R3921 |
T6393 |
T6386 |
parataxis |
ECL,using |
R3922 |
T6394 |
T6393 |
punct |
),ECL |
R3923 |
T6395 |
T6396 |
punct |
(,PIERCE |
R3924 |
T6396 |
T6386 |
parataxis |
PIERCE,using |
R3925 |
T6397 |
T6396 |
punct |
),PIERCE |
R3926 |
T6398 |
T6383 |
punct |
.,carried |
R3927 |
T6400 |
T6401 |
nsubj |
Lane,probed |
R3928 |
T6402 |
T6400 |
nummod |
1,Lane |
R3929 |
T6403 |
T6401 |
punct |
", ",probed |
R3930 |
T6404 |
T6401 |
prep |
with,probed |
R3931 |
T6405 |
T6404 |
pobj |
antibody,with |
R3932 |
T6406 |
T6405 |
acl |
generated,antibody |
R3933 |
T6407 |
T6406 |
agent |
by,generated |
R3934 |
T6408 |
T6409 |
amod |
conserved,peptide |
R3935 |
T6409 |
T6407 |
pobj |
peptide,by |
R3936 |
T6410 |
T6401 |
punct |
.,probed |
R3937 |
T6412 |
T6413 |
prep |
From,band |
R3938 |
T6414 |
T6412 |
pobj |
top,From |
R3939 |
T6415 |
T6412 |
prep |
to,From |
R3940 |
T6416 |
T6415 |
pobj |
bottom,to |
R3941 |
T6417 |
T6413 |
punct |
", ",band |
R3942 |
T6418 |
T6413 |
det |
each,band |
R3943 |
T6419 |
T6413 |
acl |
corresponding,band |
R3944 |
T6420 |
T6419 |
prep |
to,corresponding |
R3945 |
T6421 |
T6420 |
pobj |
Acdp1,to |
R3946 |
T6422 |
T6423 |
punct |
(,kD |
R3947 |
T6423 |
T6421 |
parataxis |
kD,Acdp1 |
R3948 |
T6424 |
T6423 |
nummod |
115,kD |
R3949 |
T6425 |
T6423 |
punct |
),kD |
R3950 |
T6426 |
T6421 |
punct |
", ",Acdp1 |
R3951 |
T6427 |
T6421 |
conj |
Acdp2,Acdp1 |
R3952 |
T6428 |
T6429 |
punct |
(,kD |
R3953 |
T6429 |
T6427 |
parataxis |
kD,Acdp2 |
R3954 |
T6430 |
T6429 |
nummod |
100,kD |
R3955 |
T6431 |
T6429 |
punct |
),kD |
R3956 |
T6432 |
T6427 |
punct |
", ",Acdp2 |
R3957 |
T6433 |
T6427 |
conj |
Acdp4,Acdp2 |
R3958 |
T6434 |
T6435 |
punct |
(,kD |
R3959 |
T6435 |
T6433 |
parataxis |
kD,Acdp4 |
R3960 |
T6436 |
T6435 |
nummod |
90,kD |
R3961 |
T6437 |
T6435 |
punct |
),kD |
R3962 |
T6438 |
T6433 |
cc |
and,Acdp4 |
R3963 |
T6439 |
T6433 |
conj |
Acdp3,Acdp4 |
R3964 |
T6440 |
T6441 |
punct |
(,kD |
R3965 |
T6441 |
T6439 |
parataxis |
kD,Acdp3 |
R3966 |
T6442 |
T6441 |
nummod |
80,kD |
R3967 |
T6443 |
T6441 |
punct |
),kD |
R3968 |
T6444 |
T6413 |
punct |
.,band |
R3969 |
T6446 |
T6447 |
nmod |
Lane,2 |
R3970 |
T6447 |
T6448 |
nsubj |
2,probed |
R3971 |
T6449 |
T6447 |
cc |
and,2 |
R3972 |
T6450 |
T6447 |
conj |
3,2 |
R3973 |
T6451 |
T6448 |
punct |
", ",probed |
R3974 |
T6452 |
T6448 |
prep |
with,probed |
R3975 |
T6453 |
T6454 |
det |
the,antibodies |
R3976 |
T6454 |
T6452 |
pobj |
antibodies,with |
R3977 |
T6455 |
T6454 |
compound |
Acdp1,antibodies |
R3978 |
T6456 |
T6454 |
acl |
generated,antibodies |
R3979 |
T6457 |
T6456 |
agent |
by,generated |
R3980 |
T6458 |
T6459 |
npadvmod |
N,terminal |
R3981 |
T6459 |
T6461 |
amod |
terminal,peptides |
R3982 |
T6460 |
T6459 |
punct |
-,terminal |
R3983 |
T6461 |
T6457 |
pobj |
peptides,by |
R3984 |
T6462 |
T6459 |
cc |
and,terminal |
R3985 |
T6463 |
T6464 |
npadvmod |
C,terminal |
R3986 |
T6464 |
T6459 |
conj |
terminal,terminal |
R3987 |
T6465 |
T6464 |
punct |
-,terminal |
R3988 |
T6466 |
T6456 |
punct |
", ",generated |
R3989 |
T6467 |
T6456 |
advmod |
respectively,generated |
R3990 |
T6468 |
T6448 |
punct |
.,probed |
R3991 |
T6470 |
T6471 |
dep |
Fig.,analysis |
R3992 |
T6472 |
T6470 |
nummod |
6B,Fig. |
R3993 |
T6473 |
T6471 |
punct |
: ,analysis |
R3994 |
T6474 |
T6471 |
compound |
Immunoblot,analysis |
R3995 |
T6475 |
T6471 |
prep |
of,analysis |
R3996 |
T6476 |
T6475 |
pobj |
Acdp1,of |
R3997 |
T6477 |
T6471 |
prep |
in,analysis |
R3998 |
T6478 |
T6479 |
nmod |
HEK293,cells |
R3999 |
T6479 |
T6477 |
pobj |
cells,in |
R4000 |
T6480 |
T6478 |
punct |
", ",HEK293 |
R4001 |
T6481 |
T6478 |
conj |
3T3,HEK293 |
R4002 |
T6482 |
T6481 |
cc |
and,3T3 |
R4003 |
T6483 |
T6481 |
conj |
PC12,3T3 |
R4004 |
T6484 |
T6471 |
punct |
.,analysis |
R4005 |
T6486 |
T6487 |
det |
The,blots |
R4006 |
T6487 |
T6488 |
nsubjpass |
blots,probed |
R4007 |
T6489 |
T6488 |
auxpass |
were,probed |
R4008 |
T6490 |
T6488 |
prep |
with,probed |
R4009 |
T6491 |
T6492 |
det |
the,antibody |
R4010 |
T6492 |
T6490 |
pobj |
antibody,with |
R4011 |
T6493 |
T6492 |
prep |
against,antibody |
R4012 |
T6494 |
T6495 |
det |
the,terminus |
R4013 |
T6495 |
T6493 |
pobj |
terminus,against |
R4014 |
T6496 |
T6495 |
compound |
C,terminus |
R4015 |
T6497 |
T6495 |
punct |
-,terminus |
R4016 |
T6498 |
T6495 |
prep |
of,terminus |
R4017 |
T6499 |
T6498 |
pobj |
Acdp,of |
R4018 |
T6500 |
T6499 |
punct |
-,Acdp |
R4019 |
T6501 |
T6499 |
nummod |
1,Acdp |
R4020 |
T6502 |
T6488 |
punct |
.,probed |
R4021 |
T6504 |
T6505 |
dep |
Lane,μg |
R4022 |
T6506 |
T6504 |
nummod |
1,Lane |
R4023 |
T6507 |
T6505 |
punct |
", ",μg |
R4024 |
T6508 |
T6505 |
nummod |
10,μg |
R4025 |
T6509 |
T6505 |
prep |
of,μg |
R4026 |
T6510 |
T6511 |
compound |
HEK293,lysates |
R4027 |
T6511 |
T6509 |
pobj |
lysates,of |
R4028 |
T6512 |
T6511 |
compound |
cell,lysates |
R4029 |
T6513 |
T6505 |
punct |
.,μg |
R4030 |
T6515 |
T6516 |
nmod |
Lane,2 |
R4031 |
T6516 |
T6517 |
dep |
2,μg |
R4032 |
T6518 |
T6516 |
cc |
and,2 |
R4033 |
T6519 |
T6516 |
conj |
3,2 |
R4034 |
T6520 |
T6517 |
punct |
", ",μg |
R4035 |
T6521 |
T6517 |
nummod |
100,μg |
R4036 |
T6522 |
T6517 |
prep |
of,μg |
R4037 |
T6523 |
T6524 |
nmod |
3T3,lysates |
R4038 |
T6524 |
T6522 |
pobj |
lysates,of |
R4039 |
T6525 |
T6523 |
cc |
and,3T3 |
R4040 |
T6526 |
T6523 |
conj |
PC12,3T3 |
R4041 |
T6527 |
T6524 |
compound |
cell,lysates |
R4042 |
T6528 |
T6517 |
punct |
.,μg |
R4043 |
T6553 |
T6554 |
amod |
Subcellular,localization |
R4044 |
T6555 |
T6554 |
prep |
of,localization |
R4045 |
T6556 |
T6555 |
pobj |
Acdp1,of |
R4046 |
T6557 |
T6554 |
prep |
in,localization |
R4047 |
T6558 |
T6559 |
compound |
hippocampus,neurons |
R4048 |
T6559 |
T6557 |
pobj |
neurons,in |
R4049 |
T6560 |
T6554 |
punct |
.,localization |
R4050 |
T6562 |
T6563 |
det |
A,series |
R4051 |
T6564 |
T6563 |
prep |
of,series |
R4052 |
T6565 |
T6566 |
amod |
confocal,images |
R4053 |
T6566 |
T6564 |
pobj |
images,of |
R4054 |
T6567 |
T6563 |
prep |
from,series |
R4055 |
T6568 |
T6569 |
det |
a,neuron |
R4056 |
T6569 |
T6567 |
pobj |
neuron,from |
R4057 |
T6570 |
T6569 |
amod |
cultured,neuron |
R4058 |
T6571 |
T6569 |
acl |
stained,neuron |
R4059 |
T6572 |
T6571 |
prep |
with,stained |
R4060 |
T6573 |
T6574 |
det |
an,antibody |
R4061 |
T6574 |
T6572 |
pobj |
antibody,with |
R4062 |
T6575 |
T6574 |
amod |
anti-Acdp1,antibody |
R4063 |
T6576 |
T6563 |
punct |
.,series |
R4064 |
T6578 |
T6579 |
det |
The,step |
R4065 |
T6579 |
T6580 |
nsubj |
step,is |
R4066 |
T6581 |
T6579 |
prep |
of,step |
R4067 |
T6582 |
T6583 |
det |
each,section |
R4068 |
T6583 |
T6581 |
pobj |
section,of |
R4069 |
T6584 |
T6583 |
compound |
imaging,section |
R4070 |
T6585 |
T6586 |
nummod |
0.5,μm |
R4071 |
T6586 |
T6580 |
attr |
μm,is |
R4072 |
T6587 |
T6580 |
punct |
", ",is |
R4073 |
T6588 |
T6580 |
prep |
from,is |
R4074 |
T6589 |
T6590 |
det |
the,surface |
R4075 |
T6590 |
T6588 |
pobj |
surface,from |
R4076 |
T6591 |
T6590 |
prep |
of,surface |
R4077 |
T6592 |
T6593 |
det |
the,neuron |
R4078 |
T6593 |
T6591 |
pobj |
neuron,of |
R4079 |
T6594 |
T6595 |
punct |
(,μm |
R4080 |
T6595 |
T6590 |
parataxis |
μm,surface |
R4081 |
T6596 |
T6595 |
nummod |
0,μm |
R4082 |
T6597 |
T6595 |
punct |
),μm |
R4083 |
T6598 |
T6588 |
prep |
to,from |
R4084 |
T6599 |
T6600 |
det |
the,plan |
R4085 |
T6600 |
T6598 |
pobj |
plan,to |
R4086 |
T6601 |
T6600 |
amod |
middle,plan |
R4087 |
T6602 |
T6603 |
punct |
(,μm |
R4088 |
T6603 |
T6600 |
parataxis |
μm,plan |
R4089 |
T6604 |
T6603 |
nummod |
4.5,μm |
R4090 |
T6605 |
T6603 |
punct |
),μm |
R4091 |
T6606 |
T6580 |
punct |
.,is |