PMC:3395577 / 21398-21831 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3395577","sourcedb":"PMC","sourceid":"3395577","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3395577","text":"Bacterial Strains, Plasmids, Growth Media\nThermoanaerobacterium thermosaccharolyticum DSM 571 was purchased from DSMZ (http://www.dsmz.de). It was grown anaerobically at 60°C as described previously [17]. Escherichia coli JM109 and JM109(DE3) was grown at 37°C in Luria-Bertani medium (LB) and supplemented with ampicillin when required. The expression vectors pET-20b (Novagen) were employed as cloning vector and expression vector.","divisions":[{"label":"Title","span":{"begin":0,"end":41}}],"tracks":[{"project":"MicrobeTaxon","denotations":[{"id":"T48","span":{"begin":0,"end":9},"obj":"2"},{"id":"T49","span":{"begin":42,"end":93},"obj":"1517"},{"id":"T50","span":{"begin":205,"end":221},"obj":"562"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"attributes":[{"subj":"T48","pred":"source","obj":"MicrobeTaxon"},{"subj":"T49","pred":"source","obj":"MicrobeTaxon"},{"subj":"T50","pred":"source","obj":"MicrobeTaxon"}]},{"project":"2_test","denotations":[{"id":"22571470-21464600-7770710","span":{"begin":200,"end":202},"obj":"21464600"}],"attributes":[{"subj":"22571470-21464600-7770710","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"MicrobeTaxon","color":"#ecce93","default":true},{"id":"2_test","color":"#b493ec"}]}]}}