PMC:3395577 / 20630-21373 JSONTXT

{"project":"MicrobeTaxon","denotations":[{"id":"T46","span":{"begin":103,"end":135},"obj":"1517"},{"id":"T47","span":{"begin":192,"end":199},"obj":"562"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Conclusion\nWith this study, we successfully over-expressed the novel β-glucosidase (BGL) gene bgl from T. thermosaccharolyticum DSM 571 by replacing the rare codons with the optimal codons in E. coli. The Phylogenies analysis showed that the BGL had close relationship with the β-Glucosidases from thermophile, and was distant from the other glucose-tolerant β-Glucosidases. As compared on the enzyme properties, the BGL was higher tolerant to glucose and cellobiose, more efficient in hydrolysis of cellibiose, more thermal stability than β-glucosidases from other microorganisms. Thus, this study provides a useful novel β-glucosidase, which may be used to improve the enzymatic conversion of cellulosic to glucose through synergetic action."}