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PMC:3359311 / 32752-33166 JSONTXT

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test2

Id Subject Object Predicate Lexical cue
T12138 204-224 Protein denotes alkaline phosphatase

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T12207 6-10 P05412 denotes AP-1
T12208 260-264 P05412 denotes AP-1

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T12139 204-224 Protein denotes alkaline phosphatase

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T12293 0-5 Protein denotes NF-κB
T12294 6-10 Protein denotes AP-1
T12295 204-224 Protein denotes alkaline phosphatase
T12296 254-259 Protein denotes NF-κB
T12297 260-264 Protein denotes AP-1

events-check-again

Id Subject Object Predicate Lexical cue
T12292 204-224 Protein denotes alkaline phosphatase

GO-CC

Id Subject Object Predicate Lexical cue
T12289 59-64 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T12288 213-224 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-BP

Id Subject Object Predicate Lexical cue
T12287 213-224 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T12209 0-5 NNP denotes NF-κB
T12210 5-6 NNP denotes /
T12211 6-10 NNP denotes AP-1
T12212 11-19 NNP denotes Activity
T12213 20-25 NNP denotes Assay
T12214 26-38 NNPS denotes Supernatants
T12215 39-43 IN denotes from
T12216 44-49 CD denotes THP-1
T12217 50-58 NN denotes reporter
T12218 59-64 NNS denotes cells
T12219 65-69 VBD denotes were
T12220 70-79 VBN denotes incubated
T12221 80-84 IN denotes with
T12222 85-96 JJ denotes Quanti-Blue
T12223 97-106 NN denotes substrate
T12224 107-108 -LRB- denotes (
T12225 108-117 NNP denotes Invivogen
T12226 117-118 -RRB- denotes )
T12227 119-121 IN denotes at
T12228 122-124 CD denotes 37
T12229 124-125 CD denotes °
T12230 125-126 NNP denotes C
T12231 127-130 CC denotes and
T12232 131-138 VBN denotes allowed
T12233 139-141 TO denotes to
T12234 142-149 VB denotes develop
T12235 150-153 IN denotes for
T12236 154-156 CD denotes 16
T12237 157-159 CD denotes 18
T12238 160-165 NNS denotes hours
T12239 165-166 . denotes .
T12240 167-178 JJ denotes Quanti-Blue
T12241 179-187 VBZ denotes contains
T12242 188-189 DT denotes a
T12243 190-199 NN denotes substrate
T12244 200-203 IN denotes for
T12245 204-212 JJ denotes alkaline
T12246 213-224 NN denotes phosphatase
T12247 225-228 CC denotes and
T12248 229-236 NNS denotes changes
T12249 237-239 IN denotes in
T12250 240-243 DT denotes the
T12251 244-250 NN denotes amount
T12252 251-253 IN denotes of
T12253 254-259 NNP denotes NF-κB
T12254 259-260 NNP denotes /
T12255 260-264 NNP denotes AP-1
T12256 265-273 NN denotes activity
T12257 274-278 VBD denotes were
T12258 279-289 VBN denotes quantified
T12259 290-292 IN denotes by
T12260 293-300 JJ denotes optical
T12261 301-308 NN denotes density
T12262 309-310 -LRB- denotes (
T12263 310-311 FW denotes λ
T12264 312-313 SYM denotes =
T12265 314-317 CD denotes 655
T12266 317-318 -RRB- denotes )
T12267 319-327 VBN denotes measured
T12268 328-333 VBG denotes using
T12269 334-335 DT denotes a
T12270 336-346 NNP denotes SpectraMax
T12271 347-350 CD denotes 384
T12272 351-355 NNP denotes Plus
T12273 356-361 NN denotes plate
T12274 362-368 NN denotes reader
T12275 369-372 CC denotes and
T12276 373-380 NNP denotes SoftMax
T12277 381-384 FW denotes Pro
T12278 385-393 NN denotes software
T12279 394-395 -LRB- denotes (
T12280 395-404 JJ denotes Molecular
T12281 405-412 NNPS denotes Devices
T12282 412-413 -RRB- denotes )
T12283 413-414 . denotes .
R10809 T12209 T12214 compound NF-κB,Supernatants
R10810 T12210 T12214 compound /,Supernatants
R10811 T12211 T12214 compound AP-1,Supernatants
R10812 T12212 T12214 compound Activity,Supernatants
R10813 T12213 T12214 compound Assay,Supernatants
R10814 T12214 T12220 nsubjpass Supernatants,incubated
R10815 T12215 T12214 prep from,Supernatants
R10816 T12216 T12218 nummod THP-1,cells
R10817 T12217 T12218 compound reporter,cells
R10818 T12218 T12215 pobj cells,from
R10819 T12219 T12220 auxpass were,incubated
R10820 T12220 T12220 ROOT incubated,incubated
R10821 T12221 T12220 prep with,incubated
R10822 T12222 T12223 amod Quanti-Blue,substrate
R10823 T12223 T12221 pobj substrate,with
R10824 T12224 T12225 punct (,Invivogen
R10825 T12225 T12223 appos Invivogen,substrate
R10826 T12226 T12225 punct ),Invivogen
R10827 T12227 T12220 prep at,incubated
R10828 T12228 T12229 nummod 37,°
R10829 T12229 T12230 nummod °,C
R10830 T12230 T12227 pobj C,at
R10831 T12231 T12220 cc and,incubated
R10832 T12232 T12220 conj allowed,incubated
R10833 T12233 T12234 aux to,develop
R10834 T12234 T12232 xcomp develop,allowed
R10835 T12235 T12234 prep for,develop
R10836 T12236 T12235 pobj 16,for
R10837 T12237 T12238 nummod 18,hours
R10838 T12238 T12236 appos hours,16
R10839 T12239 T12220 punct .,incubated
R10840 T12240 T12241 nsubj Quanti-Blue,contains
R10841 T12241 T12241 ROOT contains,contains
R10842 T12242 T12243 det a,substrate
R10843 T12243 T12241 dobj substrate,contains
R10844 T12244 T12243 prep for,substrate
R10845 T12245 T12246 amod alkaline,phosphatase
R10846 T12246 T12244 pobj phosphatase,for
R10847 T12247 T12246 cc and,phosphatase
R10848 T12248 T12258 nsubjpass changes,quantified
R10849 T12249 T12248 prep in,changes
R10850 T12250 T12251 det the,amount
R10851 T12251 T12249 pobj amount,in
R10852 T12252 T12251 prep of,amount
R10853 T12253 T12255 compound NF-κB,AP-1
R10854 T12254 T12255 compound /,AP-1
R10855 T12255 T12256 compound AP-1,activity
R10856 T12256 T12252 pobj activity,of
R10857 T12257 T12258 auxpass were,quantified
R10858 T12258 T12243 relcl quantified,substrate
R10859 T12259 T12258 agent by,quantified
R10860 T12260 T12261 amod optical,density
R10861 T12261 T12259 pobj density,by
R10862 T12262 T12265 punct (,655
R10863 T12263 T12265 nmod λ,655
R10864 T12264 T12265 punct =,655
R10865 T12265 T12261 appos 655,density
R10866 T12266 T12261 punct ),density
R10867 T12267 T12243 acl measured,substrate
R10868 T12268 T12267 advcl using,measured
R10869 T12269 T12274 det a,reader
R10870 T12270 T12272 nmod SpectraMax,Plus
R10871 T12271 T12272 nummod 384,Plus
R10872 T12272 T12274 nmod Plus,reader
R10873 T12273 T12274 compound plate,reader
R10874 T12274 T12268 dobj reader,using
R10875 T12275 T12274 cc and,reader
R10876 T12276 T12278 nmod SoftMax,software
R10877 T12277 T12278 amod Pro,software
R10878 T12278 T12274 conj software,reader
R10879 T12279 T12281 punct (,Devices
R10880 T12280 T12281 compound Molecular,Devices
R10881 T12281 T12278 appos Devices,software
R10882 T12282 T12241 punct ),contains
R10883 T12283 T12241 punct .,contains

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T12143 0-10 NN denotes NF-κB/AP-1
T12144 11-19 NN denotes Activity
T12145 20-25 NN denotes Assay
T12146 26-38 NN denotes Supernatants
T12147 39-43 IN denotes from
T12148 44-49 NN denotes THP-1
T12149 50-58 NN denotes reporter
T12150 59-64 NN denotes cells
T12151 65-69 VB denotes were
T12152 70-79 VB denotes incubated
T12153 80-84 IN denotes with
T12154 85-96 JJ denotes Quanti-Blue
T12155 97-106 NN denotes substrate
T12156 107-108 -LRB- denotes (
T12157 108-117 NN denotes Invivogen
T12158 117-118 -RRB- denotes )
T12159 119-121 IN denotes at
T12160 122-126 NN denotes 37°C
T12161 127-130 CC denotes and
T12162 131-138 VB denotes allowed
T12163 139-141 TO denotes to
T12164 142-149 VB denotes develop
T12165 150-153 IN denotes for
T12166 154-159 CD denotes 16–18
T12167 160-165 NN denotes hours
T12168 167-178 NN denotes Quanti-Blue
T12169 179-187 VB denotes contains
T12170 188-189 DT denotes a
T12171 190-199 NN denotes substrate
T12172 200-203 IN denotes for
T12173 204-212 NN denotes alkaline
T12174 213-224 NN denotes phosphatase
T12175 225-228 CC denotes and
T12176 229-236 NN denotes changes
T12177 237-239 IN denotes in
T12178 240-243 DT denotes the
T12179 244-250 NN denotes amount
T12180 251-253 IN denotes of
T12181 254-264 NN denotes NF-κB/AP-1
T12182 265-273 NN denotes activity
T12183 274-278 VB denotes were
T12184 279-289 VB denotes quantified
T12185 290-292 IN denotes by
T12186 293-300 JJ denotes optical
T12187 301-308 NN denotes density
T12188 309-310 -LRB- denotes (
T12189 310-317 CD denotes λ = 655
T12190 317-318 -RRB- denotes )
T12191 319-327 VB denotes measured
T12192 328-333 VB denotes using
T12193 334-335 DT denotes a
T12194 336-346 NN denotes SpectraMax
T12195 347-350 CD denotes 384
T12196 351-355 NN denotes Plus
T12197 356-361 NN denotes plate
T12198 362-368 NN denotes reader
T12199 369-372 CC denotes and
T12200 373-380 NNP denotes SoftMax
T12201 381-384 NN denotes Pro
T12202 385-393 NN denotes software
T12203 394-395 -LRB- denotes (
T12204 395-404 NNP denotes Molecular
T12205 405-412 NNP denotes Devices
T12206 412-413 -RRB- denotes )
R10744 T12145 T12143 arg1Of Assay,NF-κB/AP-1
R10745 T12145 T12144 arg1Of Assay,Activity
R10746 T12146 T12147 arg1Of Supernatants,from
R10747 T12146 T12151 arg1Of Supernatants,were
R10748 T12146 T12152 arg2Of Supernatants,incubated
R10749 T12146 T12162 arg2Of Supernatants,allowed
R10750 T12146 T12164 arg1Of Supernatants,develop
R10751 T12150 T12147 arg2Of cells,from
R10752 T12150 T12148 arg1Of cells,THP-1
R10753 T12150 T12149 arg1Of cells,reporter
R10754 T12152 T12153 arg1Of incubated,with
R10755 T12152 T12161 arg1Of incubated,and
R10756 T12155 T12153 arg2Of substrate,with
R10757 T12155 T12154 arg1Of substrate,Quanti-Blue
R10758 T12155 T12156 arg1Of substrate,(
R10759 T12155 T12159 arg1Of substrate,at
R10760 T12157 T12156 arg2Of Invivogen,(
R10761 T12158 T12156 arg3Of ),(
R10762 T12160 T12159 arg2Of 37°C,at
R10763 T12161 T12151 arg2Of and,were
R10764 T12162 T12161 arg2Of allowed,and
R10765 T12164 T12162 arg3Of develop,allowed
R10766 T12164 T12163 arg1Of develop,to
R10767 T12164 T12165 arg1Of develop,for
R10768 T12167 T12165 arg2Of hours,for
R10769 T12167 T12166 arg1Of hours,16–18
R10770 T12168 T12169 arg1Of Quanti-Blue,contains
R10771 T12169 T12175 arg1Of contains,and
R10772 T12171 T12169 arg2Of substrate,contains
R10773 T12171 T12170 arg1Of substrate,a
R10774 T12171 T12172 arg1Of substrate,for
R10775 T12174 T12172 arg2Of phosphatase,for
R10776 T12174 T12173 arg1Of phosphatase,alkaline
R10777 T12176 T12177 arg1Of changes,in
R10778 T12176 T12183 arg1Of changes,were
R10779 T12176 T12184 arg2Of changes,quantified
R10780 T12179 T12177 arg2Of amount,in
R10781 T12179 T12178 arg1Of amount,the
R10782 T12179 T12180 arg1Of amount,of
R10783 T12182 T12180 arg2Of activity,of
R10784 T12182 T12181 arg1Of activity,NF-κB/AP-1
R10785 T12184 T12175 arg2Of quantified,and
R10786 T12184 T12183 arg2Of quantified,were
R10787 T12184 T12192 modOf quantified,using
R10788 T12187 T12184 arg1Of density,quantified
R10789 T12187 T12185 arg2Of density,by
R10790 T12187 T12186 arg1Of density,optical
R10791 T12187 T12188 arg1Of density,(
R10792 T12187 T12191 arg2Of density,measured
R10793 T12189 T12188 arg2Of λ = 655,(
R10794 T12190 T12188 arg3Of ),(
R10795 T12198 T12193 arg1Of reader,a
R10796 T12198 T12194 arg1Of reader,SpectraMax
R10797 T12198 T12195 arg1Of reader,384
R10798 T12198 T12196 arg1Of reader,Plus
R10799 T12198 T12197 arg1Of reader,plate
R10800 T12198 T12199 arg1Of reader,and
R10801 T12199 T12192 arg2Of and,using
R10802 T12202 T12199 arg2Of software,and
R10803 T12202 T12200 arg1Of software,SoftMax
R10804 T12202 T12201 arg1Of software,Pro
R10805 T12202 T12203 arg1Of software,(
R10806 T12205 T12203 arg2Of Devices,(
R10807 T12205 T12204 arg1Of Devices,Molecular
R10808 T12206 T12203 arg3Of ),(

sentences

Id Subject Object Predicate Lexical cue
T12140 0-25 Sentence denotes NF-κB/AP-1 Activity Assay
T12141 26-166 Sentence denotes Supernatants from THP-1 reporter cells were incubated with Quanti-Blue substrate (Invivogen) at 37°C and allowed to develop for 16–18 hours.
T12142 167-414 Sentence denotes Quanti-Blue contains a substrate for alkaline phosphatase and changes in the amount of NF-κB/AP-1 activity were quantified by optical density (λ = 655) measured using a SpectraMax 384 Plus plate reader and SoftMax Pro software (Molecular Devices).
T226 0-25 Sentence denotes NF-κB/AP-1 Activity Assay
T227 26-166 Sentence denotes Supernatants from THP-1 reporter cells were incubated with Quanti-Blue substrate (Invivogen) at 37°C and allowed to develop for 16–18 hours.
T228 167-414 Sentence denotes Quanti-Blue contains a substrate for alkaline phosphatase and changes in the amount of NF-κB/AP-1 activity were quantified by optical density (λ = 655) measured using a SpectraMax 384 Plus plate reader and SoftMax Pro software (Molecular Devices).