PMC:3359311 / 30800-31777
Annnotations
bionlp-st-ge-2016-uniprot
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11550 | 481-486 | P01584 | denotes | IL-1β |
| T11551 | 501-505 | P25963 | denotes | IκBα |
| T11552 | 527-534 | P60709 | denotes | β-actin |
bionlp-st-ge-2016-reference
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11378 | 501-505 | Protein | denotes | IκBα |
| T11379 | 527-534 | Protein | denotes | β-actin |
bionlp-st-ge-2016-reference-tees
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11748 | 126-155 | Protein | denotes | Halt protease and phosphatase |
| T11749 | 501-534 | Protein | denotes | IκBα (Cell Signaling), or β-actin |
| T11750 | 796-809 | Protein | denotes | serum albumin |
events-check-again
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11746 | 501-505 | Protein | denotes | IκBα |
| T11747 | 527-534 | Protein | denotes | β-actin |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11736 | 21-26 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T11737 | 372-381 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membranes |
| T11738 | 621-631 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibodies |
| T11739 | 621-631 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibodies |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11740 | 144-155 | http://purl.obolibrary.org/obo/GO_0016791 | denotes | phosphatase |
| T11741 | 621-631 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibodies |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11735 | 144-155 | http://purl.obolibrary.org/obo/GO_0016791 | denotes | phosphatase |
UBERON-AE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11377 | 796-801 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
bionlp-st-ge-2016-spacy-parsed
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11553 | 0-14 | VBG | denotes | Immunoblotting |
| T11554 | 15-16 | CD | denotes | 1 |
| T11555 | 16-17 | CD | denotes | × |
| T11556 | 17-20 | CD | denotes | 106 |
| T11557 | 21-26 | NNS | denotes | cells |
| T11558 | 27-31 | VBD | denotes | were |
| T11559 | 32-38 | VBN | denotes | seeded |
| T11560 | 39-41 | IN | denotes | in |
| T11561 | 42-49 | JJ | denotes | 12-well |
| T11562 | 50-56 | NNS | denotes | plates |
| T11563 | 56-57 | , | denotes | , |
| T11564 | 58-68 | VBN | denotes | stimulated |
| T11565 | 69-71 | IN | denotes | as |
| T11566 | 72-81 | VBN | denotes | indicated |
| T11567 | 81-82 | , | denotes | , |
| T11568 | 83-86 | CC | denotes | and |
| T11569 | 87-92 | VBD | denotes | lysed |
| T11570 | 93-95 | IN | denotes | in |
| T11571 | 96-100 | NNP | denotes | RIPA |
| T11572 | 101-107 | NN | denotes | buffer |
| T11573 | 108-120 | VBN | denotes | supplemented |
| T11574 | 121-125 | IN | denotes | with |
| T11575 | 126-130 | NNP | denotes | Halt |
| T11576 | 131-139 | NN | denotes | protease |
| T11577 | 140-143 | CC | denotes | and |
| T11578 | 144-155 | NN | denotes | phosphatase |
| T11579 | 156-165 | NN | denotes | inhibitor |
| T11580 | 166-174 | NN | denotes | cocktail |
| T11581 | 175-176 | -LRB- | denotes | ( |
| T11582 | 176-182 | NNP | denotes | Thermo |
| T11583 | 183-193 | NNP | denotes | Scientific |
| T11584 | 193-194 | -RRB- | denotes | ) |
| T11585 | 194-195 | . | denotes | . |
| T11586 | 196-203 | NN | denotes | Protein |
| T11587 | 204-218 | NNS | denotes | concentrations |
| T11588 | 219-223 | VBD | denotes | were |
| T11589 | 224-234 | VBN | denotes | determined |
| T11590 | 235-237 | IN | denotes | by |
| T11591 | 238-246 | NNP | denotes | Bradford |
| T11592 | 247-252 | NN | denotes | assay |
| T11593 | 253-254 | -LRB- | denotes | ( |
| T11594 | 254-260 | NNP | denotes | Thermo |
| T11595 | 261-271 | NNP | denotes | Scientific |
| T11596 | 271-272 | -RRB- | denotes | ) |
| T11597 | 272-273 | . | denotes | . |
| T11598 | 274-281 | NNS | denotes | Lysates |
| T11599 | 282-286 | VBD | denotes | were |
| T11600 | 287-295 | VBN | denotes | resolved |
| T11601 | 296-298 | IN | denotes | by |
| T11602 | 299-314 | NNS | denotes | electrophoreses |
| T11603 | 315-317 | IN | denotes | on |
| T11604 | 318-320 | CD | denotes | 10 |
| T11605 | 320-321 | NN | denotes | % |
| T11606 | 322-340 | JJ | denotes | SDS-polyacrylamide |
| T11607 | 341-345 | NNS | denotes | gels |
| T11608 | 346-349 | CC | denotes | and |
| T11609 | 350-361 | VBN | denotes | transferred |
| T11610 | 362-366 | IN | denotes | onto |
| T11611 | 367-371 | NNP | denotes | PVDF |
| T11612 | 372-381 | NNS | denotes | membranes |
| T11613 | 382-383 | -LRB- | denotes | ( |
| T11614 | 383-392 | NNP | denotes | Millipore |
| T11615 | 392-393 | -RRB- | denotes | ) |
| T11616 | 393-394 | . | denotes | . |
| T11617 | 395-400 | NNS | denotes | Blots |
| T11618 | 401-405 | VBD | denotes | were |
| T11619 | 406-413 | VBN | denotes | blocked |
| T11620 | 414-417 | IN | denotes | for |
| T11621 | 418-419 | CD | denotes | 1 |
| T11622 | 420-424 | NN | denotes | hour |
| T11623 | 425-427 | IN | denotes | at |
| T11624 | 428-432 | NN | denotes | room |
| T11625 | 433-444 | NN | denotes | temperature |
| T11626 | 445-448 | CC | denotes | and |
| T11627 | 449-455 | VBD | denotes | probed |
| T11628 | 456-465 | JJ | denotes | overnight |
| T11629 | 466-468 | IN | denotes | at |
| T11630 | 469-470 | CD | denotes | 4 |
| T11631 | 470-471 | CD | denotes | ° |
| T11632 | 471-472 | NNP | denotes | C |
| T11633 | 473-476 | IN | denotes | for |
| T11634 | 477-486 | NNP | denotes | pro-IL-1β |
| T11635 | 487-488 | -LRB- | denotes | ( |
| T11636 | 488-493 | NNP | denotes | Santa |
| T11637 | 494-498 | NNP | denotes | Cruz |
| T11638 | 498-499 | -RRB- | denotes | ) |
| T11639 | 499-500 | , | denotes | , |
| T11640 | 501-505 | NNP | denotes | IκBα |
| T11641 | 506-507 | -LRB- | denotes | ( |
| T11642 | 507-511 | NNP | denotes | Cell |
| T11643 | 512-521 | NNP | denotes | Signaling |
| T11644 | 521-522 | -RRB- | denotes | ) |
| T11645 | 522-523 | , | denotes | , |
| T11646 | 524-526 | CC | denotes | or |
| T11647 | 527-534 | NN | denotes | β-actin |
| T11648 | 535-536 | -LRB- | denotes | ( |
| T11649 | 536-540 | NNP | denotes | Cell |
| T11650 | 541-550 | NNP | denotes | Signaling |
| T11651 | 550-551 | -RRB- | denotes | ) |
| T11652 | 551-552 | . | denotes | . |
| T11653 | 553-558 | NNS | denotes | Blots |
| T11654 | 559-563 | VBD | denotes | were |
| T11655 | 564-576 | RB | denotes | subsequently |
| T11656 | 577-583 | VBN | denotes | probed |
| T11657 | 584-588 | IN | denotes | with |
| T11658 | 589-610 | JJ | denotes | fluorescently-labeled |
| T11659 | 611-620 | JJ | denotes | secondary |
| T11660 | 621-631 | NNS | denotes | antibodies |
| T11661 | 631-632 | , | denotes | , |
| T11662 | 633-638 | NNP | denotes | IRDye |
| T11663 | 638-639 | NNP | denotes | ® |
| T11664 | 640-643 | CD | denotes | 680 |
| T11665 | 644-646 | CC | denotes | or |
| T11666 | 647-652 | CD | denotes | 800CW |
| T11667 | 653-654 | -LRB- | denotes | ( |
| T11668 | 654-660 | NNP | denotes | LI-COR |
| T11669 | 661-672 | NNPS | denotes | Biosciences |
| T11670 | 672-673 | -RRB- | denotes | ) |
| T11671 | 674-677 | IN | denotes | for |
| T11672 | 678-679 | CD | denotes | 1 |
| T11673 | 680-684 | NN | denotes | hour |
| T11674 | 684-685 | . | denotes | . |
| T11675 | 686-690 | DT | denotes | Both |
| T11676 | 691-699 | VBG | denotes | blocking |
| T11677 | 700-703 | CC | denotes | and |
| T11678 | 704-711 | VBG | denotes | probing |
| T11679 | 712-717 | NNS | denotes | steps |
| T11680 | 718-722 | VBD | denotes | were |
| T11681 | 723-730 | VBN | denotes | carried |
| T11682 | 731-734 | RP | denotes | out |
| T11683 | 735-737 | IN | denotes | in |
| T11684 | 738-751 | JJ | denotes | tris-buffered |
| T11685 | 752-758 | NN | denotes | saline |
| T11686 | 759-760 | -LRB- | denotes | ( |
| T11687 | 760-761 | NNP | denotes | G |
| T11688 | 762-773 | NNPS | denotes | Biosciences |
| T11689 | 773-774 | -RRB- | denotes | ) |
| T11690 | 775-785 | VBG | denotes | containing |
| T11691 | 786-787 | CD | denotes | 5 |
| T11692 | 787-788 | NN | denotes | % |
| T11693 | 789-795 | JJ | denotes | bovine |
| T11694 | 796-801 | NN | denotes | serum |
| T11695 | 802-809 | NN | denotes | albumin |
| T11696 | 810-813 | CC | denotes | and |
| T11697 | 814-817 | CD | denotes | 0.1 |
| T11698 | 817-818 | NN | denotes | % |
| T11699 | 819-824 | NNP | denotes | TWEEN |
| T11700 | 825-827 | CD | denotes | 20 |
| T11701 | 828-829 | -LRB- | denotes | ( |
| T11702 | 829-839 | NN | denotes | Calbiochem |
| T11703 | 839-840 | -RRB- | denotes | ) |
| T11704 | 840-841 | . | denotes | . |
| T11705 | 842-847 | NNS | denotes | Blots |
| T11706 | 848-852 | VBD | denotes | were |
| T11707 | 853-859 | VBN | denotes | imaged |
| T11708 | 860-862 | IN | denotes | on |
| T11709 | 863-864 | DT | denotes | a |
| T11710 | 865-871 | NNP | denotes | LI-COR |
| T11711 | 872-879 | NNP | denotes | Odyssey |
| T11712 | 880-888 | VBD | denotes | infrared |
| T11713 | 889-896 | NN | denotes | imaging |
| T11714 | 897-903 | NN | denotes | system |
| T11715 | 904-905 | -LRB- | denotes | ( |
| T11716 | 905-911 | NNP | denotes | LI-COR |
| T11717 | 912-923 | NNPS | denotes | Biosciences |
| T11718 | 923-924 | -RRB- | denotes | ) |
| T11719 | 925-928 | CC | denotes | and |
| T11720 | 929-939 | VBD | denotes | quantified |
| T11721 | 940-945 | VBG | denotes | using |
| T11722 | 946-949 | DT | denotes | the |
| T11723 | 950-958 | VBN | denotes | included |
| T11724 | 959-967 | NN | denotes | analysis |
| T11725 | 968-976 | NN | denotes | software |
| T11726 | 976-977 | . | denotes | . |
| R10220 | T11553 | T11559 | advcl | Immunoblotting,seeded |
| R10221 | T11554 | T11557 | nummod | 1,cells |
| R10222 | T11555 | T11557 | nummod | ×,cells |
| R10223 | T11556 | T11557 | nummod | 106,cells |
| R10224 | T11557 | T11559 | nsubjpass | cells,seeded |
| R10225 | T11558 | T11559 | auxpass | were,seeded |
| R10226 | T11559 | T11559 | ROOT | seeded,seeded |
| R10227 | T11560 | T11559 | prep | in,seeded |
| R10228 | T11561 | T11562 | amod | 12-well,plates |
| R10229 | T11562 | T11560 | pobj | plates,in |
| R10230 | T11563 | T11559 | punct | ",",seeded |
| R10231 | T11564 | T11559 | conj | stimulated,seeded |
| R10232 | T11565 | T11566 | mark | as,indicated |
| R10233 | T11566 | T11564 | advcl | indicated,stimulated |
| R10234 | T11567 | T11564 | punct | ",",stimulated |
| R10235 | T11568 | T11564 | cc | and,stimulated |
| R10236 | T11569 | T11564 | conj | lysed,stimulated |
| R10237 | T11570 | T11569 | prep | in,lysed |
| R10238 | T11571 | T11572 | compound | RIPA,buffer |
| R10239 | T11572 | T11570 | pobj | buffer,in |
| R10240 | T11573 | T11569 | dep | supplemented,lysed |
| R10241 | T11574 | T11573 | prep | with,supplemented |
| R10242 | T11575 | T11576 | compound | Halt,protease |
| R10243 | T11576 | T11574 | pobj | protease,with |
| R10244 | T11577 | T11576 | cc | and,protease |
| R10245 | T11578 | T11580 | compound | phosphatase,cocktail |
| R10246 | T11579 | T11580 | compound | inhibitor,cocktail |
| R10247 | T11580 | T11576 | conj | cocktail,protease |
| R10248 | T11581 | T11583 | punct | (,Scientific |
| R10249 | T11582 | T11583 | compound | Thermo,Scientific |
| R10250 | T11583 | T11580 | appos | Scientific,cocktail |
| R10251 | T11584 | T11583 | punct | ),Scientific |
| R10252 | T11585 | T11559 | punct | .,seeded |
| R10253 | T11586 | T11587 | compound | Protein,concentrations |
| R10254 | T11587 | T11589 | nsubjpass | concentrations,determined |
| R10255 | T11588 | T11589 | auxpass | were,determined |
| R10256 | T11589 | T11589 | ROOT | determined,determined |
| R10257 | T11590 | T11589 | agent | by,determined |
| R10258 | T11591 | T11592 | compound | Bradford,assay |
| R10259 | T11592 | T11590 | pobj | assay,by |
| R10260 | T11593 | T11595 | punct | (,Scientific |
| R10261 | T11594 | T11595 | compound | Thermo,Scientific |
| R10262 | T11595 | T11592 | appos | Scientific,assay |
| R10263 | T11596 | T11595 | punct | ),Scientific |
| R10264 | T11597 | T11589 | punct | .,determined |
| R10265 | T11598 | T11600 | nsubjpass | Lysates,resolved |
| R10266 | T11599 | T11600 | auxpass | were,resolved |
| R10267 | T11600 | T11600 | ROOT | resolved,resolved |
| R10268 | T11601 | T11600 | agent | by,resolved |
| R10269 | T11602 | T11601 | pobj | electrophoreses,by |
| R10270 | T11603 | T11602 | prep | on,electrophoreses |
| R10271 | T11604 | T11605 | nummod | 10,% |
| R10272 | T11605 | T11607 | nmod | %,gels |
| R10273 | T11606 | T11607 | amod | SDS-polyacrylamide,gels |
| R10274 | T11607 | T11603 | pobj | gels,on |
| R10275 | T11608 | T11600 | cc | and,resolved |
| R10276 | T11609 | T11600 | conj | transferred,resolved |
| R10277 | T11610 | T11609 | prep | onto,transferred |
| R10278 | T11611 | T11612 | compound | PVDF,membranes |
| R10279 | T11612 | T11610 | pobj | membranes,onto |
| R10280 | T11613 | T11614 | punct | (,Millipore |
| R10281 | T11614 | T11612 | appos | Millipore,membranes |
| R10282 | T11615 | T11612 | punct | ),membranes |
| R10283 | T11616 | T11600 | punct | .,resolved |
| R10284 | T11617 | T11619 | nsubjpass | Blots,blocked |
| R10285 | T11618 | T11619 | auxpass | were,blocked |
| R10286 | T11619 | T11619 | ROOT | blocked,blocked |
| R10287 | T11620 | T11619 | prep | for,blocked |
| R10288 | T11621 | T11622 | nummod | 1,hour |
| R10289 | T11622 | T11620 | pobj | hour,for |
| R10290 | T11623 | T11619 | prep | at,blocked |
| R10291 | T11624 | T11625 | compound | room,temperature |
| R10292 | T11625 | T11623 | pobj | temperature,at |
| R10293 | T11626 | T11619 | cc | and,blocked |
| R10294 | T11627 | T11619 | conj | probed,blocked |
| R10295 | T11628 | T11627 | dobj | overnight,probed |
| R10296 | T11629 | T11627 | prep | at,probed |
| R10297 | T11630 | T11631 | nummod | 4,° |
| R10298 | T11631 | T11629 | pobj | °,at |
| R10299 | T11632 | T11627 | conj | C,probed |
| R10300 | T11633 | T11632 | prep | for,C |
| R10301 | T11634 | T11633 | pobj | pro-IL-1β,for |
| R10302 | T11635 | T11637 | punct | (,Cruz |
| R10303 | T11636 | T11637 | compound | Santa,Cruz |
| R10304 | T11637 | T11634 | appos | Cruz,pro-IL-1β |
| R10305 | T11638 | T11637 | punct | ),Cruz |
| R10306 | T11639 | T11634 | punct | ",",pro-IL-1β |
| R10307 | T11640 | T11634 | conj | IκBα,pro-IL-1β |
| R10308 | T11641 | T11643 | punct | (,Signaling |
| R10309 | T11642 | T11643 | compound | Cell,Signaling |
| R10310 | T11643 | T11640 | parataxis | Signaling,IκBα |
| R10311 | T11644 | T11643 | punct | ),Signaling |
| R10312 | T11645 | T11643 | punct | ",",Signaling |
| R10313 | T11646 | T11640 | cc | or,IκBα |
| R10314 | T11647 | T11640 | conj | β-actin,IκBα |
| R10315 | T11648 | T11650 | punct | (,Signaling |
| R10316 | T11649 | T11650 | compound | Cell,Signaling |
| R10317 | T11650 | T11647 | appos | Signaling,β-actin |
| R10318 | T11651 | T11647 | punct | ),β-actin |
| R10319 | T11652 | T11619 | punct | .,blocked |
| R10320 | T11653 | T11656 | nsubjpass | Blots,probed |
| R10321 | T11654 | T11656 | auxpass | were,probed |
| R10322 | T11655 | T11656 | advmod | subsequently,probed |
| R10323 | T11656 | T11656 | ROOT | probed,probed |
| R10324 | T11657 | T11656 | prep | with,probed |
| R10325 | T11658 | T11660 | amod | fluorescently-labeled,antibodies |
| R10326 | T11659 | T11660 | amod | secondary,antibodies |
| R10327 | T11660 | T11657 | pobj | antibodies,with |
| R10328 | T11661 | T11656 | punct | ",",probed |
| R10329 | T11662 | T11663 | compound | IRDye,® |
| R10330 | T11663 | T11656 | punct | ®,probed |
| R10331 | T11664 | T11663 | nummod | 680,® |
| R10332 | T11665 | T11664 | cc | or,680 |
| R10333 | T11666 | T11663 | appos | 800CW,® |
| R10334 | T11667 | T11669 | punct | (,Biosciences |
| R10335 | T11668 | T11669 | compound | LI-COR,Biosciences |
| R10336 | T11669 | T11666 | appos | Biosciences,800CW |
| R10337 | T11670 | T11663 | punct | ),® |
| R10338 | T11671 | T11656 | prep | for,probed |
| R10339 | T11672 | T11673 | nummod | 1,hour |
| R10340 | T11673 | T11671 | pobj | hour,for |
| R10341 | T11674 | T11656 | punct | .,probed |
| R10342 | T11675 | T11676 | det | Both,blocking |
| R10343 | T11676 | T11681 | nsubjpass | blocking,carried |
| R10344 | T11677 | T11676 | cc | and,blocking |
| R10345 | T11678 | T11676 | conj | probing,blocking |
| R10346 | T11679 | T11681 | nsubjpass | steps,carried |
| R10347 | T11680 | T11681 | auxpass | were,carried |
| R10348 | T11681 | T11681 | ROOT | carried,carried |
| R10349 | T11682 | T11681 | prt | out,carried |
| R10350 | T11683 | T11681 | prep | in,carried |
| R10351 | T11684 | T11685 | amod | tris-buffered,saline |
| R10352 | T11685 | T11683 | pobj | saline,in |
| R10353 | T11686 | T11688 | punct | (,Biosciences |
| R10354 | T11687 | T11688 | compound | G,Biosciences |
| R10355 | T11688 | T11685 | appos | Biosciences,saline |
| R10356 | T11689 | T11688 | punct | ),Biosciences |
| R10357 | T11690 | T11681 | prep | containing,carried |
| R10358 | T11691 | T11692 | nummod | 5,% |
| R10359 | T11692 | T11695 | compound | %,albumin |
| R10360 | T11693 | T11694 | amod | bovine,serum |
| R10361 | T11694 | T11695 | compound | serum,albumin |
| R10362 | T11695 | T11690 | dobj | albumin,containing |
| R10363 | T11696 | T11695 | cc | and,albumin |
| R10364 | T11697 | T11698 | nummod | 0.1,% |
| R10365 | T11698 | T11700 | quantmod | %,20 |
| R10366 | T11699 | T11700 | quantmod | TWEEN,20 |
| R10367 | T11700 | T11695 | conj | 20,albumin |
| R10368 | T11701 | T11700 | punct | (,20 |
| R10369 | T11702 | T11700 | quantmod | Calbiochem,20 |
| R10370 | T11703 | T11700 | punct | ),20 |
| R10371 | T11704 | T11681 | punct | .,carried |
| R10372 | T11705 | T11707 | nsubjpass | Blots,imaged |
| R10373 | T11706 | T11707 | auxpass | were,imaged |
| R10374 | T11707 | T11707 | ROOT | imaged,imaged |
| R10375 | T11708 | T11707 | prep | on,imaged |
| R10376 | T11709 | T11714 | det | a,system |
| R10377 | T11710 | T11711 | compound | LI-COR,Odyssey |
| R10378 | T11711 | T11714 | nmod | Odyssey,system |
| R10379 | T11712 | T11714 | amod | infrared,system |
| R10380 | T11713 | T11714 | compound | imaging,system |
| R10381 | T11714 | T11708 | pobj | system,on |
| R10382 | T11715 | T11714 | punct | (,system |
| R10383 | T11716 | T11717 | compound | LI-COR,Biosciences |
| R10384 | T11717 | T11714 | appos | Biosciences,system |
| R10385 | T11718 | T11714 | punct | ),system |
| R10386 | T11719 | T11707 | cc | and,imaged |
| R10387 | T11720 | T11707 | conj | quantified,imaged |
| R10388 | T11721 | T11720 | xcomp | using,quantified |
| R10389 | T11722 | T11725 | det | the,software |
| R10390 | T11723 | T11725 | amod | included,software |
| R10391 | T11724 | T11725 | compound | analysis,software |
| R10392 | T11725 | T11721 | dobj | software,using |
| R10393 | T11726 | T11707 | punct | .,imaged |
pmc-enju-pas
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11388 | 0-14 | NN | denotes | Immunoblotting |
| T11389 | 15-20 | CD | denotes | 1×106 |
| T11390 | 21-26 | NN | denotes | cells |
| T11391 | 27-31 | VB | denotes | were |
| T11392 | 32-38 | VB | denotes | seeded |
| T11393 | 39-41 | IN | denotes | in |
| T11394 | 42-49 | JJ | denotes | 12-well |
| T11395 | 50-56 | NN | denotes | plates |
| T11396 | 56-57 | -COMMA- | denotes | , |
| T11397 | 58-68 | VB | denotes | stimulated |
| T11398 | 69-71 | IN | denotes | as |
| T11399 | 72-81 | VB | denotes | indicated |
| T11400 | 81-82 | -COMMA- | denotes | , |
| T11401 | 83-86 | CC | denotes | and |
| T11402 | 87-92 | VB | denotes | lysed |
| T11403 | 93-95 | IN | denotes | in |
| T11404 | 96-100 | NN | denotes | RIPA |
| T11405 | 101-107 | NN | denotes | buffer |
| T11406 | 108-120 | VB | denotes | supplemented |
| T11407 | 121-125 | IN | denotes | with |
| T11408 | 126-130 | NN | denotes | Halt |
| T11409 | 131-139 | NN | denotes | protease |
| T11410 | 140-143 | CC | denotes | and |
| T11411 | 144-155 | NN | denotes | phosphatase |
| T11412 | 156-165 | NN | denotes | inhibitor |
| T11413 | 166-174 | NN | denotes | cocktail |
| T11414 | 175-176 | -LRB- | denotes | ( |
| T11415 | 176-182 | NNP | denotes | Thermo |
| T11416 | 183-193 | NNP | denotes | Scientific |
| T11417 | 193-194 | -RRB- | denotes | ) |
| T11418 | 196-203 | NN | denotes | Protein |
| T11419 | 204-218 | NN | denotes | concentrations |
| T11420 | 219-223 | VB | denotes | were |
| T11421 | 224-234 | VB | denotes | determined |
| T11422 | 235-237 | IN | denotes | by |
| T11423 | 238-246 | NN | denotes | Bradford |
| T11424 | 247-252 | NN | denotes | assay |
| T11425 | 253-254 | -LRB- | denotes | ( |
| T11426 | 254-260 | NNP | denotes | Thermo |
| T11427 | 261-271 | NNP | denotes | Scientific |
| T11428 | 271-272 | -RRB- | denotes | ) |
| T11429 | 274-281 | NN | denotes | Lysates |
| T11430 | 282-286 | VB | denotes | were |
| T11431 | 287-295 | VB | denotes | resolved |
| T11432 | 296-298 | IN | denotes | by |
| T11433 | 299-314 | NN | denotes | electrophoreses |
| T11434 | 315-317 | IN | denotes | on |
| T11435 | 318-320 | CD | denotes | 10 |
| T11436 | 320-321 | NN | denotes | % |
| T11437 | 322-340 | NN | denotes | SDS-polyacrylamide |
| T11438 | 341-345 | NN | denotes | gels |
| T11439 | 346-349 | CC | denotes | and |
| T11440 | 350-361 | VB | denotes | transferred |
| T11441 | 362-366 | IN | denotes | onto |
| T11442 | 367-371 | NN | denotes | PVDF |
| T11443 | 372-381 | NN | denotes | membranes |
| T11444 | 382-383 | -LRB- | denotes | ( |
| T11445 | 383-392 | NNP | denotes | Millipore |
| T11446 | 392-393 | -RRB- | denotes | ) |
| T11447 | 395-400 | NN | denotes | Blots |
| T11448 | 401-405 | VB | denotes | were |
| T11449 | 406-413 | VB | denotes | blocked |
| T11450 | 414-417 | IN | denotes | for |
| T11451 | 418-419 | CD | denotes | 1 |
| T11452 | 420-424 | NN | denotes | hour |
| T11453 | 425-427 | IN | denotes | at |
| T11454 | 428-432 | NN | denotes | room |
| T11455 | 433-444 | NN | denotes | temperature |
| T11456 | 445-448 | CC | denotes | and |
| T11457 | 449-455 | VB | denotes | probed |
| T11458 | 456-465 | RB | denotes | overnight |
| T11459 | 466-468 | IN | denotes | at |
| T11460 | 469-472 | NN | denotes | 4°C |
| T11461 | 473-476 | IN | denotes | for |
| T11462 | 477-486 | NN | denotes | pro-IL-1β |
| T11463 | 487-488 | -LRB- | denotes | ( |
| T11464 | 488-493 | NNP | denotes | Santa |
| T11465 | 494-498 | NNP | denotes | Cruz |
| T11466 | 498-499 | -RRB- | denotes | ) |
| T11467 | 499-500 | -COMMA- | denotes | , |
| T11468 | 501-505 | NN | denotes | IκBα |
| T11469 | 506-507 | -LRB- | denotes | ( |
| T11470 | 507-511 | NN | denotes | Cell |
| T11471 | 512-521 | NN | denotes | Signaling |
| T11472 | 521-522 | -RRB- | denotes | ) |
| T11473 | 522-523 | -COMMA- | denotes | , |
| T11474 | 524-526 | CC | denotes | or |
| T11475 | 527-534 | NN | denotes | β-actin |
| T11476 | 535-536 | -LRB- | denotes | ( |
| T11477 | 536-540 | NN | denotes | Cell |
| T11478 | 541-550 | NN | denotes | Signaling |
| T11479 | 550-551 | -RRB- | denotes | ) |
| T11480 | 553-558 | NN | denotes | Blots |
| T11481 | 559-563 | VB | denotes | were |
| T11482 | 564-576 | RB | denotes | subsequently |
| T11483 | 577-583 | VB | denotes | probed |
| T11484 | 584-588 | IN | denotes | with |
| T11485 | 589-610 | JJ | denotes | fluorescently-labeled |
| T11486 | 611-620 | JJ | denotes | secondary |
| T11487 | 621-631 | NN | denotes | antibodies |
| T11488 | 631-632 | -COMMA- | denotes | , |
| T11489 | 633-639 | NN | denotes | IRDye® |
| T11490 | 640-643 | CD | denotes | 680 |
| T11491 | 644-646 | CC | denotes | or |
| T11492 | 647-652 | NN | denotes | 800CW |
| T11493 | 653-654 | -LRB- | denotes | ( |
| T11494 | 654-660 | NNP | denotes | LI-COR |
| T11495 | 661-672 | NNP | denotes | Biosciences |
| T11496 | 672-673 | -RRB- | denotes | ) |
| T11497 | 674-677 | IN | denotes | for |
| T11498 | 678-679 | CD | denotes | 1 |
| T11499 | 680-684 | NN | denotes | hour |
| T11500 | 686-690 | CC | denotes | Both |
| T11501 | 691-699 | VB | denotes | blocking |
| T11502 | 700-703 | CC | denotes | and |
| T11503 | 704-711 | VB | denotes | probing |
| T11504 | 712-717 | NN | denotes | steps |
| T11505 | 718-722 | VB | denotes | were |
| T11506 | 723-730 | VB | denotes | carried |
| T11507 | 731-734 | RP | denotes | out |
| T11508 | 735-737 | IN | denotes | in |
| T11509 | 738-751 | JJ | denotes | tris-buffered |
| T11510 | 752-758 | NN | denotes | saline |
| T11511 | 759-760 | -LRB- | denotes | ( |
| T11512 | 760-761 | NNP | denotes | G |
| T11513 | 762-773 | NNP | denotes | Biosciences |
| T11514 | 773-774 | -RRB- | denotes | ) |
| T11515 | 775-785 | VB | denotes | containing |
| T11516 | 786-787 | CD | denotes | 5 |
| T11517 | 787-788 | NN | denotes | % |
| T11518 | 789-795 | JJ | denotes | bovine |
| T11519 | 796-801 | NN | denotes | serum |
| T11520 | 802-809 | NN | denotes | albumin |
| T11521 | 810-813 | CC | denotes | and |
| T11522 | 814-817 | CD | denotes | 0.1 |
| T11523 | 817-818 | NN | denotes | % |
| T11524 | 819-824 | NN | denotes | TWEEN |
| T11525 | 825-827 | CD | denotes | 20 |
| T11526 | 828-829 | -LRB- | denotes | ( |
| T11527 | 829-839 | NN | denotes | Calbiochem |
| T11528 | 839-840 | -RRB- | denotes | ) |
| T11529 | 842-847 | NN | denotes | Blots |
| T11530 | 848-852 | VB | denotes | were |
| T11531 | 853-859 | VB | denotes | imaged |
| T11532 | 860-862 | IN | denotes | on |
| T11533 | 863-864 | DT | denotes | a |
| T11534 | 865-871 | NNP | denotes | LI-COR |
| T11535 | 872-879 | NNP | denotes | Odyssey |
| T11536 | 880-888 | VB | denotes | infrared |
| T11537 | 889-896 | NN | denotes | imaging |
| T11538 | 897-903 | NN | denotes | system |
| T11539 | 904-905 | -LRB- | denotes | ( |
| T11540 | 905-911 | NNP | denotes | LI-COR |
| T11541 | 912-923 | NNP | denotes | Biosciences |
| T11542 | 923-924 | -RRB- | denotes | ) |
| T11543 | 925-928 | CC | denotes | and |
| T11544 | 929-939 | VB | denotes | quantified |
| T11545 | 940-945 | VB | denotes | using |
| T11546 | 946-949 | DT | denotes | the |
| T11547 | 950-958 | VB | denotes | included |
| T11548 | 959-967 | NN | denotes | analysis |
| T11549 | 968-976 | NN | denotes | software |
| R10054 | T11390 | T11389 | arg1Of | cells,1×106 |
| R10055 | T11390 | T11391 | arg1Of | cells,were |
| R10056 | T11390 | T11392 | arg2Of | cells,seeded |
| R10057 | T11390 | T11402 | arg2Of | cells,lysed |
| R10058 | T11392 | T11393 | arg1Of | seeded,in |
| R10059 | T11392 | T11401 | arg1Of | seeded,and |
| R10060 | T11395 | T11393 | arg2Of | plates,in |
| R10061 | T11395 | T11394 | arg1Of | plates,12-well |
| R10062 | T11395 | T11396 | arg1Of | plates,"," |
| R10063 | T11395 | T11397 | arg2Of | plates,stimulated |
| R10064 | T11397 | T11398 | arg1Of | stimulated,as |
| R10065 | T11399 | T11398 | arg2Of | indicated,as |
| R10066 | T11401 | T11391 | arg2Of | and,were |
| R10067 | T11401 | T11400 | arg1Of | and,"," |
| R10068 | T11402 | T11401 | arg2Of | lysed,and |
| R10069 | T11402 | T11403 | arg1Of | lysed,in |
| R10070 | T11405 | T11403 | arg2Of | buffer,in |
| R10071 | T11405 | T11404 | arg1Of | buffer,RIPA |
| R10072 | T11405 | T11406 | arg2Of | buffer,supplemented |
| R10073 | T11406 | T11407 | arg1Of | supplemented,with |
| R10074 | T11409 | T11408 | arg1Of | protease,Halt |
| R10075 | T11409 | T11410 | arg1Of | protease,and |
| R10076 | T11410 | T11407 | arg2Of | and,with |
| R10077 | T11413 | T11410 | arg2Of | cocktail,and |
| R10078 | T11413 | T11411 | arg1Of | cocktail,phosphatase |
| R10079 | T11413 | T11412 | arg1Of | cocktail,inhibitor |
| R10080 | T11413 | T11414 | arg1Of | cocktail,( |
| R10081 | T11416 | T11414 | arg2Of | Scientific,( |
| R10082 | T11416 | T11415 | arg1Of | Scientific,Thermo |
| R10083 | T11417 | T11414 | arg3Of | ),( |
| R10084 | T11419 | T11418 | arg1Of | concentrations,Protein |
| R10085 | T11419 | T11420 | arg1Of | concentrations,were |
| R10086 | T11419 | T11421 | arg2Of | concentrations,determined |
| R10087 | T11421 | T11420 | arg2Of | determined,were |
| R10088 | T11424 | T11421 | arg1Of | assay,determined |
| R10089 | T11424 | T11422 | arg2Of | assay,by |
| R10090 | T11424 | T11423 | arg1Of | assay,Bradford |
| R10091 | T11424 | T11425 | arg1Of | assay,( |
| R10092 | T11427 | T11425 | arg2Of | Scientific,( |
| R10093 | T11427 | T11426 | arg1Of | Scientific,Thermo |
| R10094 | T11428 | T11425 | arg3Of | ),( |
| R10095 | T11429 | T11430 | arg1Of | Lysates,were |
| R10096 | T11429 | T11431 | arg2Of | Lysates,resolved |
| R10097 | T11429 | T11440 | arg2Of | Lysates,transferred |
| R10098 | T11431 | T11432 | arg1Of | resolved,by |
| R10099 | T11431 | T11439 | arg1Of | resolved,and |
| R10100 | T11433 | T11432 | arg2Of | electrophoreses,by |
| R10101 | T11433 | T11434 | arg1Of | electrophoreses,on |
| R10102 | T11435 | T11436 | arg1Of | 10,% |
| R10103 | T11438 | T11434 | arg2Of | gels,on |
| R10104 | T11438 | T11435 | arg1Of | gels,10 |
| R10105 | T11438 | T11437 | arg1Of | gels,SDS-polyacrylamide |
| R10106 | T11439 | T11430 | arg2Of | and,were |
| R10107 | T11440 | T11439 | arg2Of | transferred,and |
| R10108 | T11440 | T11441 | arg1Of | transferred,onto |
| R10109 | T11443 | T11441 | arg2Of | membranes,onto |
| R10110 | T11443 | T11442 | arg1Of | membranes,PVDF |
| R10111 | T11443 | T11444 | arg1Of | membranes,( |
| R10112 | T11445 | T11444 | arg2Of | Millipore,( |
| R10113 | T11446 | T11444 | arg3Of | ),( |
| R10114 | T11447 | T11448 | arg1Of | Blots,were |
| R10115 | T11447 | T11449 | arg2Of | Blots,blocked |
| R10116 | T11447 | T11457 | arg1Of | Blots,probed |
| R10117 | T11449 | T11448 | arg2Of | blocked,were |
| R10118 | T11449 | T11450 | arg1Of | blocked,for |
| R10119 | T11449 | T11456 | arg1Of | blocked,and |
| R10120 | T11452 | T11450 | arg2Of | hour,for |
| R10121 | T11452 | T11451 | arg1Of | hour,1 |
| R10122 | T11452 | T11453 | arg1Of | hour,at |
| R10123 | T11455 | T11453 | arg2Of | temperature,at |
| R10124 | T11455 | T11454 | arg1Of | temperature,room |
| R10125 | T11457 | T11456 | arg2Of | probed,and |
| R10126 | T11457 | T11458 | arg1Of | probed,overnight |
| R10127 | T11457 | T11459 | arg1Of | probed,at |
| R10128 | T11460 | T11459 | arg2Of | 4°C,at |
| R10129 | T11460 | T11461 | arg1Of | 4°C,for |
| R10130 | T11462 | T11463 | arg1Of | pro-IL-1β,( |
| R10131 | T11462 | T11467 | arg1Of | pro-IL-1β,"," |
| R10132 | T11465 | T11463 | arg2Of | Cruz,( |
| R10133 | T11465 | T11464 | arg1Of | Cruz,Santa |
| R10134 | T11466 | T11463 | arg3Of | ),( |
| R10135 | T11467 | T11474 | arg1Of | ",",or |
| R10136 | T11468 | T11467 | arg2Of | IκBα,"," |
| R10137 | T11468 | T11469 | arg1Of | IκBα,( |
| R10138 | T11471 | T11469 | arg2Of | Signaling,( |
| R10139 | T11471 | T11470 | arg1Of | Signaling,Cell |
| R10140 | T11472 | T11469 | arg3Of | ),( |
| R10141 | T11474 | T11461 | arg2Of | or,for |
| R10142 | T11474 | T11473 | arg1Of | or,"," |
| R10143 | T11475 | T11474 | arg2Of | β-actin,or |
| R10144 | T11475 | T11476 | arg1Of | β-actin,( |
| R10145 | T11478 | T11476 | arg2Of | Signaling,( |
| R10146 | T11478 | T11477 | arg1Of | Signaling,Cell |
| R10147 | T11479 | T11476 | arg3Of | ),( |
| R10148 | T11480 | T11481 | arg1Of | Blots,were |
| R10149 | T11480 | T11483 | arg2Of | Blots,probed |
| R10150 | T11483 | T11481 | arg2Of | probed,were |
| R10151 | T11483 | T11482 | arg1Of | probed,subsequently |
| R10152 | T11483 | T11484 | arg1Of | probed,with |
| R10153 | T11483 | T11497 | arg1Of | probed,for |
| R10154 | T11487 | T11484 | arg2Of | antibodies,with |
| R10155 | T11487 | T11485 | arg1Of | antibodies,fluorescently-labeled |
| R10156 | T11487 | T11486 | arg1Of | antibodies,secondary |
| R10157 | T11487 | T11488 | arg1Of | antibodies,"," |
| R10158 | T11489 | T11490 | arg1Of | IRDye®,680 |
| R10159 | T11489 | T11491 | arg1Of | IRDye®,or |
| R10160 | T11491 | T11488 | arg2Of | or,"," |
| R10161 | T11492 | T11491 | arg2Of | 800CW,or |
| R10162 | T11492 | T11493 | arg1Of | 800CW,( |
| R10163 | T11495 | T11493 | arg2Of | Biosciences,( |
| R10164 | T11495 | T11494 | arg1Of | Biosciences,LI-COR |
| R10165 | T11496 | T11493 | arg3Of | ),( |
| R10166 | T11499 | T11497 | arg2Of | hour,for |
| R10167 | T11499 | T11498 | arg1Of | hour,1 |
| R10168 | T11504 | T11501 | arg1Of | steps,blocking |
| R10169 | T11504 | T11502 | arg1Of | steps,and |
| R10170 | T11504 | T11503 | arg1Of | steps,probing |
| R10171 | T11504 | T11505 | arg1Of | steps,were |
| R10172 | T11504 | T11506 | arg2Of | steps,carried |
| R10173 | T11506 | T11500 | arg1Of | carried,Both |
| R10174 | T11506 | T11505 | arg2Of | carried,were |
| R10175 | T11506 | T11507 | arg1Of | carried,out |
| R10176 | T11506 | T11508 | arg1Of | carried,in |
| R10177 | T11510 | T11508 | arg2Of | saline,in |
| R10178 | T11510 | T11509 | arg1Of | saline,tris-buffered |
| R10179 | T11510 | T11511 | arg1Of | saline,( |
| R10180 | T11510 | T11515 | arg1Of | saline,containing |
| R10181 | T11513 | T11511 | arg2Of | Biosciences,( |
| R10182 | T11513 | T11512 | arg1Of | Biosciences,G |
| R10183 | T11514 | T11511 | arg3Of | ),( |
| R10184 | T11516 | T11517 | arg1Of | 5,% |
| R10185 | T11520 | T11516 | arg1Of | albumin,5 |
| R10186 | T11520 | T11518 | arg1Of | albumin,bovine |
| R10187 | T11520 | T11519 | arg1Of | albumin,serum |
| R10188 | T11520 | T11521 | arg1Of | albumin,and |
| R10189 | T11521 | T11515 | arg2Of | and,containing |
| R10190 | T11522 | T11523 | arg1Of | 0.1,% |
| R10191 | T11524 | T11521 | arg2Of | TWEEN,and |
| R10192 | T11524 | T11522 | arg1Of | TWEEN,0.1 |
| R10193 | T11524 | T11525 | arg1Of | TWEEN,20 |
| R10194 | T11524 | T11526 | arg1Of | TWEEN,( |
| R10195 | T11527 | T11526 | arg2Of | Calbiochem,( |
| R10196 | T11528 | T11526 | arg3Of | ),( |
| R10197 | T11529 | T11530 | arg1Of | Blots,were |
| R10198 | T11529 | T11531 | arg2Of | Blots,imaged |
| R10199 | T11529 | T11544 | arg2Of | Blots,quantified |
| R10200 | T11529 | T11545 | arg1Of | Blots,using |
| R10201 | T11531 | T11532 | arg1Of | imaged,on |
| R10202 | T11531 | T11543 | arg1Of | imaged,and |
| R10203 | T11535 | T11532 | arg2Of | Odyssey,on |
| R10204 | T11535 | T11533 | arg1Of | Odyssey,a |
| R10205 | T11535 | T11534 | arg1Of | Odyssey,LI-COR |
| R10206 | T11538 | T11531 | arg3Of | system,imaged |
| R10207 | T11538 | T11536 | arg2Of | system,infrared |
| R10208 | T11538 | T11537 | arg1Of | system,imaging |
| R10209 | T11538 | T11539 | arg1Of | system,( |
| R10210 | T11541 | T11539 | arg2Of | Biosciences,( |
| R10211 | T11541 | T11540 | arg1Of | Biosciences,LI-COR |
| R10212 | T11542 | T11539 | arg3Of | ),( |
| R10213 | T11543 | T11530 | arg2Of | and,were |
| R10214 | T11544 | T11543 | arg2Of | quantified,and |
| R10215 | T11545 | T11544 | arg3Of | using,quantified |
| R10216 | T11549 | T11545 | arg2Of | software,using |
| R10217 | T11549 | T11546 | arg1Of | software,the |
| R10218 | T11549 | T11547 | arg2Of | software,included |
| R10219 | T11549 | T11548 | arg1Of | software,analysis |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T11380 | 0-14 | Sentence | denotes | Immunoblotting |
| T11381 | 15-195 | Sentence | denotes | 1×106 cells were seeded in 12-well plates, stimulated as indicated, and lysed in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific). |
| T11382 | 196-273 | Sentence | denotes | Protein concentrations were determined by Bradford assay (Thermo Scientific). |
| T11383 | 274-394 | Sentence | denotes | Lysates were resolved by electrophoreses on 10% SDS-polyacrylamide gels and transferred onto PVDF membranes (Millipore). |
| T11384 | 395-552 | Sentence | denotes | Blots were blocked for 1 hour at room temperature and probed overnight at 4°C for pro-IL-1β (Santa Cruz), IκBα (Cell Signaling), or β-actin (Cell Signaling). |
| T11385 | 553-685 | Sentence | denotes | Blots were subsequently probed with fluorescently-labeled secondary antibodies, IRDye® 680 or 800CW (LI-COR Biosciences) for 1 hour. |
| T11386 | 686-841 | Sentence | denotes | Both blocking and probing steps were carried out in tris-buffered saline (G Biosciences) containing 5% bovine serum albumin and 0.1% TWEEN 20 (Calbiochem). |
| T11387 | 842-977 | Sentence | denotes | Blots were imaged on a LI-COR Odyssey infrared imaging system (LI-COR Biosciences) and quantified using the included analysis software. |
| T210 | 0-14 | Sentence | denotes | Immunoblotting |
| T211 | 15-195 | Sentence | denotes | 1×106 cells were seeded in 12-well plates, stimulated as indicated, and lysed in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific). |
| T212 | 196-273 | Sentence | denotes | Protein concentrations were determined by Bradford assay (Thermo Scientific). |
| T213 | 274-394 | Sentence | denotes | Lysates were resolved by electrophoreses on 10% SDS-polyacrylamide gels and transferred onto PVDF membranes (Millipore). |
| T214 | 395-552 | Sentence | denotes | Blots were blocked for 1 hour at room temperature and probed overnight at 4°C for pro-IL-1β (Santa Cruz), IκBα (Cell Signaling), or β-actin (Cell Signaling). |
| T215 | 553-685 | Sentence | denotes | Blots were subsequently probed with fluorescently-labeled secondary antibodies, IRDye® 680 or 800CW (LI-COR Biosciences) for 1 hour. |
| T216 | 686-841 | Sentence | denotes | Both blocking and probing steps were carried out in tris-buffered saline (G Biosciences) containing 5% bovine serum albumin and 0.1% TWEEN 20 (Calbiochem). |
| T217 | 842-977 | Sentence | denotes | Blots were imaged on a LI-COR Odyssey infrared imaging system (LI-COR Biosciences) and quantified using the included analysis software. |