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PMC:3359311 / 25931-28194 JSONTXT

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test2

Id Subject Object Predicate Lexical cue
T9445 973-984 Protein denotes transferrin
T9446 993-1000 Protein denotes insulin
T9447 1658-1671 Protein denotes serum albumin

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T9879 562-566 P05412 denotes AP-1
T9880 1387-1391 P05412 denotes AP-1

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T9451 973-984 Protein denotes transferrin
T9452 993-1000 Protein denotes insulin
T9453 1658-1671 Protein denotes serum albumin

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T10375 181-186 Protein denotes ΔF508
T10376 191-197 Protein denotes W1282X
T10377 166-176 Gene_expression denotes expressing
T10378 166-176 Gene_expression denotes expressing
T10379 322-326 Protein denotes CFTR
T10380 434-447 Protein denotes CFTR genotype
T10381 449-453 Protein denotes THP1
T10382 492-522 Protein denotes embryonic alkaline phosphatase
T10383 524-528 Protein denotes SEAP
T10384 552-557 Protein denotes NF-κB
T10385 562-578 Protein denotes AP-1 (Invivogen)
T10386 473-480 Gene_expression denotes express
T10387 473-480 Gene_expression denotes express
T10388 483-491 Localization denotes secreted
T10389 483-491 Localization denotes secreted
T10390 539-548 Positive_regulation denotes inducible
T10391 539-548 Positive_regulation denotes inducible
T10392 903-924 Protein denotes supplement bullet kit
T10393 926-929 Protein denotes EGF
T10394 973-984 Protein denotes transferrin
T10395 986-1000 Protein denotes bovine insulin
T10396 1253-1257 Protein denotes THP1
T10397 1296-1300 Protein denotes RPMI
T10398 1376-1383 Protein denotes SEAP NF
T10399 1384-1386 Protein denotes κB
T10400 1387-1400 Protein denotes AP-1 reporter
T10401 1361-1371 Gene_expression denotes expressing
T10402 1361-1371 Gene_expression denotes expressing
T10403 1361-1371 Gene_expression denotes expressing
T10404 1597-1599 Protein denotes S9
T10405 1604-1609 Protein denotes IB3-1
T10406 1651-1671 Protein denotes bovine serum albumin
T10407 1685-1696 Protein denotes fibronectin
T10408 1810-1826 Protein denotes collagen type IV
R9119 T10375 T10377 themeOf ΔF508,expressing
R9120 T10376 T10378 themeOf W1282X,expressing
R9121 T10382 T10386 themeOf embryonic alkaline phosphatase,express
R9122 T10382 T10388 themeOf embryonic alkaline phosphatase,secreted
R9123 T10382 T10390 themeOf embryonic alkaline phosphatase,inducible
R9124 T10382 T10391 themeOf embryonic alkaline phosphatase,inducible
R9125 T10383 T10387 themeOf SEAP,express
R9126 T10383 T10389 themeOf SEAP,secreted
R9127 T10384 T10390 causeOf NF-κB,inducible
R9128 T10385 T10391 causeOf AP-1 (Invivogen),inducible
R9129 T10398 T10401 themeOf SEAP NF,expressing
R9130 T10399 T10402 themeOf κB,expressing
R9131 T10400 T10403 themeOf AP-1 reporter,expressing

events-check-again

Id Subject Object Predicate Lexical cue
T10372 973-984 Protein denotes transferrin
T10373 993-1000 Protein denotes insulin
T10374 1658-1671 Protein denotes serum albumin

GO-CC

Id Subject Object Predicate Lexical cue
T10347 0-4 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T10348 63-68 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10349 132-137 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10350 268-273 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10351 284-289 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10352 377-382 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10353 460-465 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10354 684-689 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10355 844-849 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10356 1264-1269 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10357 1355-1360 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10358 1524-1529 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10359 1915-1920 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10360 1945-1950 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10361 1971-1976 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10362 2137-2142 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10363 2165-2170 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T10364 511-522 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T10365 926-929 http://purl.obolibrary.org/obo/GO_0005154 denotes EGF

GO-BP

Id Subject Object Predicate Lexical cue
T10345 511-522 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T10346 1982-2014 http://purl.obolibrary.org/obo/GO_0030225 denotes differentiated into a macrophage

UBERON-AE

Id Subject Object Predicate Lexical cue
T9448 773-778 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T9449 872-877 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T9450 1658-1663 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T9881 0-4 NNP denotes Cell
T9882 5-12 NNP denotes Culture
T9883 13-15 NNP denotes CF
T9884 16-17 -LRB- denotes (
T9885 17-22 NNP denotes IB3-1
T9886 23-26 CC denotes and
T9887 27-33 NNP denotes CuFi-1
T9888 33-34 -RRB- denotes )
T9889 35-38 CC denotes and
T9890 39-46 NN denotes control
T9891 47-48 -LRB- denotes (
T9892 48-50 CD denotes S9
T9893 51-54 CC denotes and
T9894 55-61 NNP denotes NuLi-1
T9895 61-62 -RRB- denotes )
T9896 63-68 NNS denotes cells
T9897 69-73 VBD denotes were
T9898 74-82 VBN denotes obtained
T9899 83-87 IN denotes from
T9900 88-91 DT denotes the
T9901 92-100 JJ denotes American
T9902 101-105 NNP denotes Type
T9903 106-113 NNP denotes Culture
T9904 114-124 NNP denotes Collection
T9905 124-125 . denotes .
T9906 126-131 CD denotes IB3-1
T9907 132-137 NNS denotes cells
T9908 138-142 VBD denotes were
T9909 143-150 VBN denotes derived
T9910 151-155 IN denotes from
T9911 156-157 DT denotes a
T9912 158-165 JJ denotes patient
T9913 166-176 VBG denotes expressing
T9914 177-180 DT denotes the
T9915 181-186 NNP denotes ΔF508
T9916 187-190 CC denotes and
T9917 191-197 NNP denotes W1282X
T9918 198-207 NNS denotes mutations
T9919 208-211 CC denotes and
T9920 212-218 NN denotes CuFi-1
T9921 219-223 VBD denotes were
T9922 224-231 VBN denotes derived
T9923 232-236 IN denotes from
T9924 237-238 DT denotes a
T9925 239-244 NNP denotes ΔF508
T9926 245-255 JJ denotes homozygous
T9927 256-263 NN denotes patient
T9928 263-264 . denotes .
T9929 265-267 CD denotes S9
T9930 268-273 NNS denotes cells
T9931 274-277 VBP denotes are
T9932 278-283 JJ denotes IB3-1
T9933 284-289 NNS denotes cells
T9934 290-294 WDT denotes that
T9935 295-299 VBP denotes have
T9936 300-304 VBN denotes been
T9937 305-316 VBN denotes transfected
T9938 317-321 IN denotes with
T9939 322-326 NNP denotes CFTR
T9940 327-332 VBG denotes using
T9941 333-335 DT denotes an
T9942 336-352 JJ denotes adeno-associated
T9943 353-358 JJ denotes viral
T9944 359-365 NN denotes vector
T9945 366-369 CC denotes and
T9946 370-376 JJ denotes NuLi-1
T9947 377-382 NNS denotes cells
T9948 383-387 VBD denotes were
T9949 388-395 VBN denotes derived
T9950 396-400 IN denotes from
T9951 401-402 DT denotes a
T9952 403-410 JJ denotes patient
T9953 411-421 VBG denotes possessing
T9954 422-423 DT denotes a
T9955 424-433 JJ denotes wild-type
T9956 434-438 NNP denotes CFTR
T9957 439-447 NN denotes genotype
T9958 447-448 . denotes .
T9959 449-459 JJ denotes THP1-XBlue
T9960 460-465 NNS denotes cells
T9961 466-472 RB denotes stably
T9962 473-480 VBP denotes express
T9963 481-482 DT denotes a
T9964 483-491 JJ denotes secreted
T9965 492-501 JJ denotes embryonic
T9966 502-510 NN denotes alkaline
T9967 511-522 NN denotes phosphatase
T9968 523-524 -LRB- denotes (
T9969 524-528 NNP denotes SEAP
T9970 528-529 -RRB- denotes )
T9971 530-538 NN denotes reporter
T9972 539-548 JJ denotes inducible
T9973 549-551 IN denotes by
T9974 552-557 NNP denotes NF-κB
T9975 558-561 CC denotes and
T9976 562-566 NNP denotes AP-1
T9977 567-568 -LRB- denotes (
T9978 568-577 NNP denotes Invivogen
T9979 577-578 -RRB- denotes )
T9980 578-579 . denotes .
T9981 580-585 NNS denotes Cells
T9982 586-590 VBD denotes were
T9983 591-599 VBN denotes cultured
T9984 600-602 IN denotes as
T9985 603-614 VBN denotes recommended
T9986 615-617 IN denotes by
T9987 618-623 PRP$ denotes their
T9988 624-634 JJ denotes respective
T9989 635-644 NNS denotes suppliers
T9990 645-650 VBG denotes using
T9991 651-659 JJ denotes standard
T9992 660-669 NNS denotes protocols
T9993 669-670 . denotes .
T9994 671-673 CD denotes S9
T9995 674-677 CC denotes and
T9996 678-683 CD denotes IB3-1
T9997 684-689 NNS denotes cells
T9998 690-694 VBD denotes were
T9999 695-703 VBN denotes cultured
T10000 704-706 IN denotes in
T10001 707-712 JJ denotes basal
T10002 713-718 NN denotes LHC-8
T10003 719-720 -LRB- denotes (
T10004 720-730 NNP denotes Invitrogen
T10005 730-731 -RRB- denotes )
T10006 732-744 VBN denotes supplemented
T10007 745-749 IN denotes with
T10008 750-752 CD denotes 10
T10009 752-753 NN denotes %
T10010 754-755 -LRB- denotes (
T10011 755-758 FW denotes v/v
T10012 758-759 -RRB- denotes )
T10013 760-765 JJ denotes fetal
T10014 766-772 JJ denotes bovine
T10015 773-778 NN denotes serum
T10016 779-780 -LRB- denotes (
T10017 780-783 NNP denotes FBS
T10018 783-784 -RRB- denotes )
T10019 784-785 , denotes ,
T10020 786-787 CD denotes 2
T10021 788-790 NNP denotes mM
T10022 791-802 NNP denotes L-glutamine
T10023 802-803 , denotes ,
T10024 804-807 CC denotes and
T10025 808-809 CD denotes 1
T10026 810-812 NNP denotes mM
T10027 813-819 NN denotes sodium
T10028 820-828 NN denotes pyruvate
T10029 828-829 . denotes .
T10030 830-836 JJ denotes NuLi-1
T10031 836-837 NN denotes /
T10032 837-843 NN denotes CuFi-1
T10033 844-849 NNS denotes cells
T10034 850-854 VBD denotes were
T10035 855-863 VBN denotes cultured
T10036 864-866 IN denotes in
T10037 867-871 NNP denotes BEBM
T10038 872-882 JJ denotes serum-free
T10039 883-889 NN denotes medium
T10040 890-891 -LRB- denotes (
T10041 891-896 NNP denotes Lonza
T10042 896-897 -RRB- denotes )
T10043 898-902 IN denotes with
T10044 903-913 NN denotes supplement
T10045 914-920 NN denotes bullet
T10046 921-924 NN denotes kit
T10047 925-926 -LRB- denotes (
T10048 926-929 NNP denotes EGF
T10049 929-930 , denotes ,
T10050 931-945 NN denotes hydrocortisone
T10051 945-946 , denotes ,
T10052 947-953 JJ denotes bovine
T10053 954-963 JJ denotes pituitary
T10054 964-971 VB denotes extract
T10055 971-972 , denotes ,
T10056 973-984 NN denotes transferrin
T10057 984-985 , denotes ,
T10058 986-992 JJ denotes bovine
T10059 993-1000 NN denotes insulin
T10060 1000-1001 , denotes ,
T10061 1002-1018 NN denotes triiodothyronine
T10062 1018-1019 , denotes ,
T10063 1020-1031 NN denotes epinephrine
T10064 1031-1032 , denotes ,
T10065 1033-1041 JJ denotes retinoic
T10066 1042-1046 NN denotes acid
T10067 1046-1047 -RRB- denotes )
T10068 1047-1048 , denotes ,
T10069 1049-1050 CD denotes 2
T10070 1051-1053 NNP denotes mM
T10071 1054-1065 NNP denotes L-glutamine
T10072 1065-1066 , denotes ,
T10073 1067-1070 CC denotes and
T10074 1071-1072 CD denotes 1
T10075 1073-1075 NNP denotes mM
T10076 1076-1082 NN denotes sodium
T10077 1083-1091 NN denotes pyruvate
T10078 1091-1092 . denotes .
T10079 1093-1098 NNS denotes PBMCs
T10080 1099-1103 IN denotes from
T10081 1104-1106 NNP denotes CF
T10082 1107-1115 NNS denotes patients
T10083 1116-1119 CC denotes and
T10084 1120-1128 NNS denotes controls
T10085 1129-1133 VBD denotes were
T10086 1134-1142 VBN denotes cultured
T10087 1143-1145 IN denotes in
T10088 1146-1155 NNP denotes RPMI-1640
T10089 1156-1157 -LRB- denotes (
T10090 1157-1164 NNP denotes Hyclone
T10091 1164-1165 -RRB- denotes )
T10092 1166-1178 VBN denotes supplemented
T10093 1179-1183 IN denotes with
T10094 1184-1186 CD denotes 10
T10095 1186-1187 NN denotes %
T10096 1188-1191 NNP denotes FBS
T10097 1191-1192 , denotes ,
T10098 1193-1194 CD denotes 2
T10099 1195-1197 NNP denotes mM
T10100 1198-1209 NNP denotes L-glutamine
T10101 1209-1210 , denotes ,
T10102 1211-1214 CC denotes and
T10103 1215-1216 CD denotes 1
T10104 1217-1219 NNP denotes mM
T10105 1220-1226 NN denotes sodium
T10106 1227-1235 NN denotes pyruvate
T10107 1236-1237 -LRB- denotes (
T10108 1237-1245 JJ denotes complete
T10109 1246-1250 NNP denotes RPMI
T10110 1250-1251 -RRB- denotes )
T10111 1251-1252 . denotes .
T10112 1253-1263 JJ denotes THP1-XBlue
T10113 1264-1269 NNS denotes cells
T10114 1270-1274 VBD denotes were
T10115 1275-1283 VBN denotes cultured
T10116 1284-1286 IN denotes in
T10117 1287-1295 JJ denotes complete
T10118 1296-1300 NNP denotes RPMI
T10119 1301-1305 IN denotes with
T10120 1306-1309 DT denotes the
T10121 1310-1318 NN denotes addition
T10122 1319-1321 IN denotes of
T10123 1322-1328 NN denotes zeocin
T10124 1329-1330 -LRB- denotes (
T10125 1330-1333 CD denotes 100
T10126 1334-1336 NN denotes µg
T10127 1336-1337 NN denotes /
T10128 1337-1339 NN denotes ml
T10129 1339-1340 -RRB- denotes )
T10130 1341-1343 TO denotes to
T10131 1344-1350 VB denotes select
T10132 1351-1354 IN denotes for
T10133 1355-1360 NNS denotes cells
T10134 1361-1371 VBG denotes expressing
T10135 1372-1375 DT denotes the
T10136 1376-1380 NNP denotes SEAP
T10137 1381-1386 NNP denotes NF-κB
T10138 1386-1387 NNP denotes /
T10139 1387-1391 NNP denotes AP-1
T10140 1392-1400 NN denotes reporter
T10141 1400-1401 . denotes .
T10142 1402-1407 RB denotes Prior
T10143 1408-1410 TO denotes to
T10144 1411-1422 NN denotes stimulation
T10145 1422-1423 , denotes ,
T10146 1424-1433 JJ denotes bronchial
T10147 1434-1444 JJ denotes epithelial
T10148 1445-1449 NN denotes cell
T10149 1450-1455 NNS denotes lines
T10150 1456-1460 VBD denotes were
T10151 1461-1467 VBN denotes plated
T10152 1468-1470 IN denotes in
T10153 1471-1477 JJ denotes coated
T10154 1478-1479 NNP denotes [
T10155 1479-1481 CD denotes 62
T10156 1481-1482 NNP denotes ]
T10157 1483-1490 JJ denotes 96-well
T10158 1491-1497 NNS denotes plates
T10159 1498-1499 -LRB- denotes (
T10160 1499-1501 NNP denotes BD
T10161 1502-1513 NNPS denotes Biosciences
T10162 1513-1514 -RRB- denotes )
T10163 1515-1517 IN denotes at
T10164 1518-1519 CD denotes 3
T10165 1519-1520 CD denotes ×
T10166 1520-1523 CD denotes 104
T10167 1524-1534 NN denotes cells/well
T10168 1535-1541 IN denotes unless
T10169 1542-1551 VBN denotes indicated
T10170 1551-1552 , denotes ,
T10171 1553-1556 CC denotes and
T10172 1557-1564 VBD denotes allowed
T10173 1565-1567 TO denotes to
T10174 1568-1574 VB denotes adhere
T10175 1575-1584 JJ denotes overnight
T10176 1584-1585 . denotes .
T10177 1586-1592 NNP denotes Plates
T10178 1593-1596 IN denotes for
T10179 1597-1599 NNP denotes S9
T10180 1600-1603 CC denotes and
T10181 1604-1609 NNP denotes IB3-1
T10182 1610-1622 NNS denotes stimulations
T10183 1623-1627 VBD denotes were
T10184 1628-1634 VBN denotes coated
T10185 1635-1637 IN denotes in
T10186 1638-1639 DT denotes a
T10187 1640-1647 NN denotes mixture
T10188 1648-1650 IN denotes of
T10189 1651-1657 JJ denotes bovine
T10190 1658-1663 NN denotes serum
T10191 1664-1671 NN denotes albumin
T10192 1672-1673 -LRB- denotes (
T10193 1673-1676 CD denotes 100
T10194 1677-1679 NN denotes µg
T10195 1679-1680 NN denotes /
T10196 1680-1682 NN denotes ml
T10197 1682-1683 -RRB- denotes )
T10198 1683-1684 , denotes ,
T10199 1685-1696 NN denotes fibronectin
T10200 1697-1698 -LRB- denotes (
T10201 1698-1700 CD denotes 10
T10202 1701-1703 NN denotes µg
T10203 1703-1704 NN denotes /
T10204 1704-1706 NN denotes ml
T10205 1706-1707 -RRB- denotes )
T10206 1707-1708 , denotes ,
T10207 1709-1712 CC denotes and
T10208 1713-1719 JJ denotes bovine
T10209 1720-1727 NN denotes collage
T10210 1728-1732 NN denotes type
T10211 1733-1734 PRP denotes I
T10212 1735-1736 -LRB- denotes (
T10213 1736-1738 CD denotes 30
T10214 1739-1741 NN denotes µg
T10215 1741-1742 NN denotes /
T10216 1742-1744 NN denotes ml
T10217 1744-1745 -RRB- denotes )
T10218 1746-1747 -LRB- denotes (
T10219 1747-1749 NNP denotes BD
T10220 1750-1761 NNPS denotes Biosciences
T10221 1761-1762 -RRB- denotes )
T10222 1762-1763 . denotes .
T10223 1764-1770 NNS denotes Plates
T10224 1771-1774 IN denotes for
T10225 1775-1781 NN denotes NuLi-1
T10226 1782-1785 CC denotes and
T10227 1786-1792 NN denotes CuFi-1
T10228 1793-1797 VBD denotes were
T10229 1798-1804 VBN denotes coated
T10230 1805-1809 IN denotes with
T10231 1810-1818 NN denotes collagen
T10232 1819-1823 NN denotes type
T10233 1824-1826 NNP denotes IV
T10234 1827-1828 -LRB- denotes (
T10235 1828-1830 CD denotes 60
T10236 1831-1833 NN denotes µg
T10237 1833-1834 NN denotes /
T10238 1834-1836 NN denotes ml
T10239 1836-1837 -RRB- denotes )
T10240 1838-1839 -LRB- denotes (
T10241 1839-1844 NNP denotes Sigma
T10242 1845-1852 NNP denotes Aldrich
T10243 1852-1853 -RRB- denotes )
T10244 1853-1854 . denotes .
T10245 1855-1860 NNS denotes PBMCs
T10246 1861-1865 VBD denotes were
T10247 1866-1872 VBN denotes plated
T10248 1873-1875 IN denotes in
T10249 1876-1883 JJ denotes 96-well
T10250 1884-1890 NNS denotes plates
T10251 1891-1893 IN denotes at
T10252 1894-1895 DT denotes a
T10253 1896-1903 NN denotes density
T10254 1904-1906 IN denotes of
T10255 1907-1910 CD denotes 1.5
T10256 1910-1911 CD denotes ×
T10257 1911-1914 CD denotes 105
T10258 1915-1925 NN denotes cells/well
T10259 1926-1928 IN denotes in
T10260 1929-1932 CD denotes 200
T10261 1933-1935 NN denotes µl
T10262 1936-1937 -LRB- denotes (
T10263 1937-1940 CD denotes 7.5
T10264 1940-1941 CD denotes ×
T10265 1941-1944 CD denotes 105
T10266 1945-1953 NNP denotes cells/mL
T10267 1953-1954 -RRB- denotes )
T10268 1954-1955 . denotes .
T10269 1956-1961 CD denotes THP-1
T10270 1962-1970 NN denotes reporter
T10271 1971-1976 NNS denotes cells
T10272 1977-1981 VBD denotes were
T10273 1982-1996 VBN denotes differentiated
T10274 1997-2001 IN denotes into
T10275 2002-2003 DT denotes a
T10276 2004-2019 JJ denotes macrophage-like
T10277 2020-2029 NN denotes phenotype
T10278 2030-2035 VBG denotes using
T10279 2036-2038 CD denotes 50
T10280 2039-2044 NN denotes ng/ml
T10281 2045-2047 IN denotes of
T10282 2048-2055 NN denotes phorbol
T10283 2056-2068 JJ denotes 12-myristate
T10284 2069-2079 JJ denotes 13-acetate
T10285 2080-2081 -LRB- denotes (
T10286 2081-2084 NNP denotes PMA
T10287 2084-2085 -RRB- denotes )
T10288 2086-2087 -LRB- denotes (
T10289 2087-2092 NNP denotes Sigma
T10290 2093-2100 NNP denotes Aldrich
T10291 2100-2101 -RRB- denotes )
T10292 2102-2105 IN denotes for
T10293 2106-2108 CD denotes 24
T10294 2109-2114 NNS denotes hours
T10295 2115-2117 IN denotes at
T10296 2118-2119 DT denotes a
T10297 2120-2127 NN denotes density
T10298 2128-2130 IN denotes of
T10299 2131-2132 CD denotes 1
T10300 2132-2133 CD denotes ×
T10301 2133-2136 CD denotes 105
T10302 2137-2147 NN denotes cells/well
T10303 2148-2150 IN denotes in
T10304 2151-2154 CD denotes 200
T10305 2155-2157 NN denotes µl
T10306 2158-2159 -LRB- denotes (
T10307 2159-2160 CD denotes 5
T10308 2160-2161 CD denotes ×
T10309 2161-2164 CD denotes 105
T10310 2165-2173 NN denotes cells/ml
T10311 2173-2174 -RRB- denotes )
T10312 2174-2175 . denotes .
T10313 2176-2181 NNS denotes Cells
T10314 2182-2186 VBD denotes were
T10315 2187-2193 VBN denotes washed
T10316 2194-2198 IN denotes with
T10317 2199-2202 NNP denotes PBS
T10318 2203-2206 CC denotes and
T10319 2207-2214 VBN denotes allowed
T10320 2215-2217 TO denotes to
T10321 2218-2222 VB denotes rest
T10322 2223-2224 DT denotes a
T10323 2225-2232 JJ denotes further
T10324 2233-2235 CD denotes 42
T10325 2236-2241 NNS denotes hours
T10326 2242-2247 RB denotes prior
T10327 2248-2250 TO denotes to
T10328 2251-2262 NN denotes stimulation
T10329 2262-2263 . denotes .
R8670 T9881 T9883 compound Cell,CF
R8671 T9882 T9883 compound Culture,CF
R8672 T9883 T9898 nsubjpass CF,obtained
R8673 T9884 T9883 punct (,CF
R8674 T9885 T9896 nmod IB3-1,cells
R8675 T9886 T9885 cc and,IB3-1
R8676 T9887 T9885 conj CuFi-1,IB3-1
R8677 T9888 T9887 punct ),CuFi-1
R8678 T9889 T9885 cc and,IB3-1
R8679 T9890 T9885 conj control,IB3-1
R8680 T9891 T9890 punct (,control
R8681 T9892 T9890 appos S9,control
R8682 T9893 T9885 cc and,IB3-1
R8683 T9894 T9885 conj NuLi-1,IB3-1
R8684 T9895 T9896 punct ),cells
R8685 T9896 T9898 nsubjpass cells,obtained
R8686 T9897 T9898 auxpass were,obtained
R8687 T9898 T9898 ROOT obtained,obtained
R8688 T9899 T9898 prep from,obtained
R8689 T9900 T9904 det the,Collection
R8690 T9901 T9904 compound American,Collection
R8691 T9902 T9904 compound Type,Collection
R8692 T9903 T9904 compound Culture,Collection
R8693 T9904 T9899 pobj Collection,from
R8694 T9905 T9898 punct .,obtained
R8695 T9906 T9907 nummod IB3-1,cells
R8696 T9907 T9909 nsubjpass cells,derived
R8697 T9908 T9909 auxpass were,derived
R8698 T9909 T9909 ROOT derived,derived
R8699 T9910 T9909 prep from,derived
R8700 T9911 T9912 det a,patient
R8701 T9912 T9910 pobj patient,from
R8702 T9913 T9912 acl expressing,patient
R8703 T9914 T9915 det the,ΔF508
R8704 T9915 T9913 dobj ΔF508,expressing
R8705 T9916 T9915 cc and,ΔF508
R8706 T9917 T9915 conj W1282X,ΔF508
R8707 T9918 T9915 conj mutations,ΔF508
R8708 T9919 T9918 cc and,mutations
R8709 T9920 T9918 conj CuFi-1,mutations
R8710 T9921 T9922 auxpass were,derived
R8711 T9922 T9909 conj derived,derived
R8712 T9923 T9922 prep from,derived
R8713 T9924 T9927 det a,patient
R8714 T9925 T9927 nmod ΔF508,patient
R8715 T9926 T9927 amod homozygous,patient
R8716 T9927 T9923 pobj patient,from
R8717 T9928 T9909 punct .,derived
R8718 T9929 T9930 nummod S9,cells
R8719 T9930 T9931 nsubj cells,are
R8720 T9931 T9931 ROOT are,are
R8721 T9932 T9933 amod IB3-1,cells
R8722 T9933 T9931 attr cells,are
R8723 T9934 T9937 nsubjpass that,transfected
R8724 T9935 T9937 aux have,transfected
R8725 T9936 T9937 auxpass been,transfected
R8726 T9937 T9933 relcl transfected,cells
R8727 T9938 T9937 prep with,transfected
R8728 T9939 T9940 nsubj CFTR,using
R8729 T9940 T9938 pcomp using,with
R8730 T9941 T9949 det an,derived
R8731 T9942 T9944 amod adeno-associated,vector
R8732 T9943 T9944 amod viral,vector
R8733 T9944 T9949 nsubjpass vector,derived
R8734 T9945 T9944 cc and,vector
R8735 T9946 T9944 conj NuLi-1,vector
R8736 T9947 T9944 conj cells,vector
R8737 T9948 T9949 auxpass were,derived
R8738 T9949 T9931 advcl derived,are
R8739 T9950 T9949 prep from,derived
R8740 T9951 T9952 det a,patient
R8741 T9952 T9950 pobj patient,from
R8742 T9953 T9952 acl possessing,patient
R8743 T9954 T9957 det a,genotype
R8744 T9955 T9957 amod wild-type,genotype
R8745 T9956 T9957 amod CFTR,genotype
R8746 T9957 T9953 dobj genotype,possessing
R8747 T9958 T9949 punct .,derived
R8748 T9959 T9960 amod THP1-XBlue,cells
R8749 T9960 T9962 nsubj cells,express
R8750 T9961 T9962 advmod stably,express
R8751 T9962 T9962 ROOT express,express
R8752 T9963 T9967 det a,phosphatase
R8753 T9964 T9967 amod secreted,phosphatase
R8754 T9965 T9967 amod embryonic,phosphatase
R8755 T9966 T9967 compound alkaline,phosphatase
R8756 T9967 T9962 dobj phosphatase,express
R8757 T9968 T9967 punct (,phosphatase
R8758 T9969 T9967 appos SEAP,phosphatase
R8759 T9970 T9967 punct ),phosphatase
R8760 T9971 T9972 compound reporter,inducible
R8761 T9972 T9967 appos inducible,phosphatase
R8762 T9973 T9972 prep by,inducible
R8763 T9974 T9973 pobj NF-κB,by
R8764 T9975 T9974 cc and,NF-κB
R8765 T9976 T9974 conj AP-1,NF-κB
R8766 T9977 T9978 punct (,Invivogen
R8767 T9978 T9976 appos Invivogen,AP-1
R8768 T9979 T9976 punct ),AP-1
R8769 T9980 T9962 punct .,express
R8770 T9981 T9983 nsubjpass Cells,cultured
R8771 T9982 T9983 auxpass were,cultured
R8772 T9983 T9983 ROOT cultured,cultured
R8773 T9984 T9985 mark as,recommended
R8774 T9985 T9983 advcl recommended,cultured
R8775 T9986 T9985 agent by,recommended
R8776 T9987 T9989 poss their,suppliers
R8777 T9988 T9989 amod respective,suppliers
R8778 T9989 T9986 pobj suppliers,by
R8779 T9990 T9989 acl using,suppliers
R8780 T9991 T9992 amod standard,protocols
R8781 T9992 T9990 dobj protocols,using
R8782 T9993 T9983 punct .,cultured
R8783 T9994 T9997 nummod S9,cells
R8784 T9995 T9994 cc and,S9
R8785 T9996 T9994 conj IB3-1,S9
R8786 T9997 T9999 nsubjpass cells,cultured
R8787 T9998 T9999 auxpass were,cultured
R8788 T9999 T9999 ROOT cultured,cultured
R8789 T10000 T9999 prep in,cultured
R8790 T10001 T10002 amod basal,LHC-8
R8791 T10002 T10000 pobj LHC-8,in
R8792 T10003 T10004 punct (,Invitrogen
R8793 T10004 T10002 appos Invitrogen,LHC-8
R8794 T10005 T9999 punct ),cultured
R8795 T10006 T9999 prep supplemented,cultured
R8796 T10007 T10006 prep with,supplemented
R8797 T10008 T10009 nummod 10,%
R8798 T10009 T10007 pobj %,with
R8799 T10010 T10011 punct (,v/v
R8800 T10011 T10009 appos v/v,%
R8801 T10012 T10011 punct ),v/v
R8802 T10013 T10015 amod fetal,serum
R8803 T10014 T10015 amod bovine,serum
R8804 T10015 T10009 appos serum,%
R8805 T10016 T10017 punct (,FBS
R8806 T10017 T10015 parataxis FBS,serum
R8807 T10018 T10017 punct ),FBS
R8808 T10019 T10015 punct ",",serum
R8809 T10020 T10022 nummod 2,L-glutamine
R8810 T10021 T10022 compound mM,L-glutamine
R8811 T10022 T10015 appos L-glutamine,serum
R8812 T10023 T10015 punct ",",serum
R8813 T10024 T10015 cc and,serum
R8814 T10025 T10026 nummod 1,mM
R8815 T10026 T10028 compound mM,pyruvate
R8816 T10027 T10028 compound sodium,pyruvate
R8817 T10028 T10015 conj pyruvate,serum
R8818 T10029 T9999 punct .,cultured
R8819 T10030 T10033 amod NuLi-1,cells
R8820 T10031 T10033 compound /,cells
R8821 T10032 T10033 compound CuFi-1,cells
R8822 T10033 T10034 nsubj cells,were
R8823 T10034 T10034 ROOT were,were
R8824 T10035 T10034 acomp cultured,were
R8825 T10036 T10035 prep in,cultured
R8826 T10037 T10039 nmod BEBM,medium
R8827 T10038 T10039 amod serum-free,medium
R8828 T10039 T10036 pobj medium,in
R8829 T10040 T10041 punct (,Lonza
R8830 T10041 T10039 appos Lonza,medium
R8831 T10042 T10035 punct ),cultured
R8832 T10043 T10034 prep with,were
R8833 T10044 T10046 compound supplement,kit
R8834 T10045 T10046 compound bullet,kit
R8835 T10046 T10043 pobj kit,with
R8836 T10047 T10046 punct (,kit
R8837 T10048 T10050 nmod EGF,hydrocortisone
R8838 T10049 T10050 punct ",",hydrocortisone
R8839 T10050 T10046 appos hydrocortisone,kit
R8840 T10051 T10050 punct ",",hydrocortisone
R8841 T10052 T10054 amod bovine,extract
R8842 T10053 T10054 amod pituitary,extract
R8843 T10054 T10050 conj extract,hydrocortisone
R8844 T10055 T10054 punct ",",extract
R8845 T10056 T10050 conj transferrin,hydrocortisone
R8846 T10057 T10056 punct ",",transferrin
R8847 T10058 T10059 amod bovine,insulin
R8848 T10059 T10056 conj insulin,transferrin
R8849 T10060 T10059 punct ",",insulin
R8850 T10061 T10059 conj triiodothyronine,insulin
R8851 T10062 T10061 punct ",",triiodothyronine
R8852 T10063 T10061 conj epinephrine,triiodothyronine
R8853 T10064 T10063 punct ",",epinephrine
R8854 T10065 T10066 amod retinoic,acid
R8855 T10066 T10063 appos acid,epinephrine
R8856 T10067 T10063 punct ),epinephrine
R8857 T10068 T10061 punct ",",triiodothyronine
R8858 T10069 T10071 nummod 2,L-glutamine
R8859 T10070 T10071 compound mM,L-glutamine
R8860 T10071 T10061 conj L-glutamine,triiodothyronine
R8861 T10072 T10071 punct ",",L-glutamine
R8862 T10073 T10071 cc and,L-glutamine
R8863 T10074 T10077 nummod 1,pyruvate
R8864 T10075 T10077 compound mM,pyruvate
R8865 T10076 T10077 compound sodium,pyruvate
R8866 T10077 T10071 conj pyruvate,L-glutamine
R8867 T10078 T10077 punct .,pyruvate
R8868 T10079 T10085 nsubj PBMCs,were
R8869 T10080 T10079 prep from,PBMCs
R8870 T10081 T10082 compound CF,patients
R8871 T10082 T10080 pobj patients,from
R8872 T10083 T10082 cc and,patients
R8873 T10084 T10082 conj controls,patients
R8874 T10085 T10085 ROOT were,were
R8875 T10086 T10085 acomp cultured,were
R8876 T10087 T10086 prep in,cultured
R8877 T10088 T10087 pobj RPMI-1640,in
R8878 T10089 T10088 punct (,RPMI-1640
R8879 T10090 T10088 appos Hyclone,RPMI-1640
R8880 T10091 T10088 punct ),RPMI-1640
R8881 T10092 T10086 conj supplemented,cultured
R8882 T10093 T10092 prep with,supplemented
R8883 T10094 T10095 nummod 10,%
R8884 T10095 T10096 compound %,FBS
R8885 T10096 T10093 pobj FBS,with
R8886 T10097 T10096 punct ",",FBS
R8887 T10098 T10100 nummod 2,L-glutamine
R8888 T10099 T10100 compound mM,L-glutamine
R8889 T10100 T10096 conj L-glutamine,FBS
R8890 T10101 T10100 punct ",",L-glutamine
R8891 T10102 T10100 cc and,L-glutamine
R8892 T10103 T10106 nummod 1,pyruvate
R8893 T10104 T10106 compound mM,pyruvate
R8894 T10105 T10106 compound sodium,pyruvate
R8895 T10106 T10100 conj pyruvate,L-glutamine
R8896 T10107 T10106 punct (,pyruvate
R8897 T10108 T10109 amod complete,RPMI
R8898 T10109 T10106 appos RPMI,pyruvate
R8899 T10110 T10106 punct ),pyruvate
R8900 T10111 T10085 punct .,were
R8901 T10112 T10113 amod THP1-XBlue,cells
R8902 T10113 T10114 nsubj cells,were
R8903 T10114 T10114 ROOT were,were
R8904 T10115 T10114 acomp cultured,were
R8905 T10116 T10115 prep in,cultured
R8906 T10117 T10118 amod complete,RPMI
R8907 T10118 T10116 pobj RPMI,in
R8908 T10119 T10115 prep with,cultured
R8909 T10120 T10121 det the,addition
R8910 T10121 T10119 pobj addition,with
R8911 T10122 T10121 prep of,addition
R8912 T10123 T10122 pobj zeocin,of
R8913 T10124 T10123 punct (,zeocin
R8914 T10125 T10126 nummod 100,µg
R8915 T10126 T10128 nmod µg,ml
R8916 T10127 T10128 compound /,ml
R8917 T10128 T10123 appos ml,zeocin
R8918 T10129 T10128 punct ),ml
R8919 T10130 T10131 aux to,select
R8920 T10131 T10115 advcl select,cultured
R8921 T10132 T10131 prep for,select
R8922 T10133 T10132 pobj cells,for
R8923 T10134 T10133 acl expressing,cells
R8924 T10135 T10140 det the,reporter
R8925 T10136 T10139 compound SEAP,AP-1
R8926 T10137 T10139 compound NF-κB,AP-1
R8927 T10138 T10139 compound /,AP-1
R8928 T10139 T10140 compound AP-1,reporter
R8929 T10140 T10134 dobj reporter,expressing
R8930 T10141 T10114 punct .,were
R8931 T10142 T10151 advmod Prior,plated
R8932 T10143 T10151 prep to,plated
R8933 T10144 T10143 pobj stimulation,to
R8934 T10145 T10151 punct ",",plated
R8935 T10146 T10149 amod bronchial,lines
R8936 T10147 T10149 amod epithelial,lines
R8937 T10148 T10149 compound cell,lines
R8938 T10149 T10151 nsubjpass lines,plated
R8939 T10150 T10151 auxpass were,plated
R8940 T10151 T10151 ROOT plated,plated
R8941 T10152 T10151 prep in,plated
R8942 T10153 T10158 amod coated,plates
R8943 T10154 T10158 nmod [,plates
R8944 T10155 T10158 nummod 62,plates
R8945 T10156 T10158 nmod ],plates
R8946 T10157 T10158 compound 96-well,plates
R8947 T10158 T10152 pobj plates,in
R8948 T10159 T10161 punct (,Biosciences
R8949 T10160 T10161 compound BD,Biosciences
R8950 T10161 T10158 appos Biosciences,plates
R8951 T10162 T10161 punct ),Biosciences
R8952 T10163 T10151 prep at,plated
R8953 T10164 T10167 nummod 3,cells/well
R8954 T10165 T10167 nummod ×,cells/well
R8955 T10166 T10167 nummod 104,cells/well
R8956 T10167 T10163 pobj cells/well,at
R8957 T10168 T10169 mark unless,indicated
R8958 T10169 T10151 advcl indicated,plated
R8959 T10170 T10151 punct ",",plated
R8960 T10171 T10151 cc and,plated
R8961 T10172 T10151 conj allowed,plated
R8962 T10173 T10174 aux to,adhere
R8963 T10174 T10172 xcomp adhere,allowed
R8964 T10175 T10174 advmod overnight,adhere
R8965 T10176 T10151 punct .,plated
R8966 T10177 T10184 nsubjpass Plates,coated
R8967 T10178 T10177 prep for,Plates
R8968 T10179 T10178 pobj S9,for
R8969 T10180 T10179 cc and,S9
R8970 T10181 T10179 conj IB3-1,S9
R8971 T10182 T10184 nsubjpass stimulations,coated
R8972 T10183 T10184 auxpass were,coated
R8973 T10184 T10184 ROOT coated,coated
R8974 T10185 T10184 prep in,coated
R8975 T10186 T10187 det a,mixture
R8976 T10187 T10185 pobj mixture,in
R8977 T10188 T10187 prep of,mixture
R8978 T10189 T10190 amod bovine,serum
R8979 T10190 T10191 compound serum,albumin
R8980 T10191 T10188 pobj albumin,of
R8981 T10192 T10191 punct (,albumin
R8982 T10193 T10194 nummod 100,µg
R8983 T10194 T10196 compound µg,ml
R8984 T10195 T10196 compound /,ml
R8985 T10196 T10191 appos ml,albumin
R8986 T10197 T10191 punct ),albumin
R8987 T10198 T10184 punct ",",coated
R8988 T10199 T10184 dep fibronectin,coated
R8989 T10200 T10199 punct (,fibronectin
R8990 T10201 T10202 nummod 10,µg
R8991 T10202 T10199 appos µg,fibronectin
R8992 T10203 T10204 compound /,ml
R8993 T10204 T10202 appos ml,µg
R8994 T10205 T10202 punct ),µg
R8995 T10206 T10199 punct ",",fibronectin
R8996 T10207 T10199 cc and,fibronectin
R8997 T10208 T10209 amod bovine,collage
R8998 T10209 T10210 compound collage,type
R8999 T10210 T10199 conj type,fibronectin
R9000 T10211 T10199 nsubj I,fibronectin
R9001 T10212 T10211 punct (,I
R9002 T10213 T10214 nummod 30,µg
R9003 T10214 T10216 compound µg,ml
R9004 T10215 T10216 compound /,ml
R9005 T10216 T10211 appos ml,I
R9006 T10217 T10211 punct ),I
R9007 T10218 T10220 punct (,Biosciences
R9008 T10219 T10220 compound BD,Biosciences
R9009 T10220 T10211 appos Biosciences,I
R9010 T10221 T10220 punct ),Biosciences
R9011 T10222 T10184 punct .,coated
R9012 T10223 T10229 nsubjpass Plates,coated
R9013 T10224 T10223 prep for,Plates
R9014 T10225 T10224 pobj NuLi-1,for
R9015 T10226 T10225 cc and,NuLi-1
R9016 T10227 T10225 conj CuFi-1,NuLi-1
R9017 T10228 T10229 auxpass were,coated
R9018 T10229 T10229 ROOT coated,coated
R9019 T10230 T10229 prep with,coated
R9020 T10231 T10232 compound collagen,type
R9021 T10232 T10233 compound type,IV
R9022 T10233 T10230 pobj IV,with
R9023 T10234 T10233 punct (,IV
R9024 T10235 T10236 nummod 60,µg
R9025 T10236 T10238 compound µg,ml
R9026 T10237 T10238 compound /,ml
R9027 T10238 T10233 appos ml,IV
R9028 T10239 T10233 punct ),IV
R9029 T10240 T10233 punct (,IV
R9030 T10241 T10242 compound Sigma,Aldrich
R9031 T10242 T10233 appos Aldrich,IV
R9032 T10243 T10233 punct ),IV
R9033 T10244 T10229 punct .,coated
R9034 T10245 T10247 nsubjpass PBMCs,plated
R9035 T10246 T10247 auxpass were,plated
R9036 T10247 T10247 ROOT plated,plated
R9037 T10248 T10247 prep in,plated
R9038 T10249 T10250 amod 96-well,plates
R9039 T10250 T10248 pobj plates,in
R9040 T10251 T10247 prep at,plated
R9041 T10252 T10253 det a,density
R9042 T10253 T10251 pobj density,at
R9043 T10254 T10253 prep of,density
R9044 T10255 T10258 nummod 1.5,cells/well
R9045 T10256 T10258 nmod ×,cells/well
R9046 T10257 T10258 nummod 105,cells/well
R9047 T10258 T10254 pobj cells/well,of
R9048 T10259 T10247 prep in,plated
R9049 T10260 T10261 nummod 200,µl
R9050 T10261 T10259 pobj µl,in
R9051 T10262 T10261 punct (,µl
R9052 T10263 T10266 nummod 7.5,cells/mL
R9053 T10264 T10266 nummod ×,cells/mL
R9054 T10265 T10266 nummod 105,cells/mL
R9055 T10266 T10261 appos cells/mL,µl
R9056 T10267 T10261 punct ),µl
R9057 T10268 T10247 punct .,plated
R9058 T10269 T10271 nummod THP-1,cells
R9059 T10270 T10271 compound reporter,cells
R9060 T10271 T10273 nsubjpass cells,differentiated
R9061 T10272 T10273 auxpass were,differentiated
R9062 T10273 T10273 ROOT differentiated,differentiated
R9063 T10274 T10273 prep into,differentiated
R9064 T10275 T10277 det a,phenotype
R9065 T10276 T10277 amod macrophage-like,phenotype
R9066 T10277 T10274 pobj phenotype,into
R9067 T10278 T10273 advcl using,differentiated
R9068 T10279 T10280 nummod 50,ng/ml
R9069 T10280 T10278 dobj ng/ml,using
R9070 T10281 T10280 prep of,ng/ml
R9071 T10282 T10284 nmod phorbol,13-acetate
R9072 T10283 T10284 amod 12-myristate,13-acetate
R9073 T10284 T10281 pobj 13-acetate,of
R9074 T10285 T10286 punct (,PMA
R9075 T10286 T10284 appos PMA,13-acetate
R9076 T10287 T10284 punct ),13-acetate
R9077 T10288 T10290 punct (,Aldrich
R9078 T10289 T10290 compound Sigma,Aldrich
R9079 T10290 T10284 appos Aldrich,13-acetate
R9080 T10291 T10280 punct ),ng/ml
R9081 T10292 T10278 prep for,using
R9082 T10293 T10294 nummod 24,hours
R9083 T10294 T10292 pobj hours,for
R9084 T10295 T10278 prep at,using
R9085 T10296 T10297 det a,density
R9086 T10297 T10295 pobj density,at
R9087 T10298 T10297 prep of,density
R9088 T10299 T10302 nummod 1,cells/well
R9089 T10300 T10302 nummod ×,cells/well
R9090 T10301 T10302 nummod 105,cells/well
R9091 T10302 T10298 pobj cells/well,of
R9092 T10303 T10278 prep in,using
R9093 T10304 T10305 nummod 200,µl
R9094 T10305 T10303 pobj µl,in
R9095 T10306 T10305 punct (,µl
R9096 T10307 T10310 nummod 5,cells/ml
R9097 T10308 T10310 nummod ×,cells/ml
R9098 T10309 T10310 nummod 105,cells/ml
R9099 T10310 T10305 appos cells/ml,µl
R9100 T10311 T10305 punct ),µl
R9101 T10312 T10273 punct .,differentiated
R9102 T10313 T10315 nsubjpass Cells,washed
R9103 T10314 T10315 auxpass were,washed
R9104 T10315 T10315 ROOT washed,washed
R9105 T10316 T10315 prep with,washed
R9106 T10317 T10316 pobj PBS,with
R9107 T10318 T10315 cc and,washed
R9108 T10319 T10315 conj allowed,washed
R9109 T10320 T10321 aux to,rest
R9110 T10321 T10319 xcomp rest,allowed
R9111 T10322 T10326 det a,prior
R9112 T10323 T10326 amod further,prior
R9113 T10324 T10325 nummod 42,hours
R9114 T10325 T10326 npadvmod hours,prior
R9115 T10326 T10321 advmod prior,rest
R9116 T10327 T10326 prep to,prior
R9117 T10328 T10327 pobj stimulation,to
R9118 T10329 T10315 punct .,washed

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T9469 0-4 NNP denotes Cell
T9470 5-12 NNP denotes Culture
T9471 13-15 NN denotes CF
T9472 16-17 -LRB- denotes (
T9473 17-22 NN denotes IB3-1
T9474 23-26 CC denotes and
T9475 27-33 NN denotes CuFi-1
T9476 33-34 -RRB- denotes )
T9477 35-38 CC denotes and
T9478 39-46 NN denotes control
T9479 47-48 -LRB- denotes (
T9480 48-50 NN denotes S9
T9481 51-54 CC denotes and
T9482 55-61 NN denotes NuLi-1
T9483 61-62 -RRB- denotes )
T9484 63-68 NN denotes cells
T9485 69-73 VB denotes were
T9486 74-82 VB denotes obtained
T9487 83-87 IN denotes from
T9488 88-91 DT denotes the
T9489 92-100 NNP denotes American
T9490 101-105 NNP denotes Type
T9491 106-113 NNP denotes Culture
T9492 114-125 NNP denotes Collection.
T9493 126-131 NN denotes IB3-1
T9494 132-137 NN denotes cells
T9495 138-142 VB denotes were
T9496 143-150 VB denotes derived
T9497 151-155 IN denotes from
T9498 156-157 DT denotes a
T9499 158-165 NN denotes patient
T9500 166-176 VB denotes expressing
T9501 177-180 DT denotes the
T9502 181-186 NN denotes ΔF508
T9503 187-190 CC denotes and
T9504 191-197 NN denotes W1282X
T9505 198-207 NN denotes mutations
T9506 208-211 CC denotes and
T9507 212-218 NN denotes CuFi-1
T9508 219-223 VB denotes were
T9509 224-231 VB denotes derived
T9510 232-236 IN denotes from
T9511 237-238 DT denotes a
T9512 239-244 NN denotes ΔF508
T9513 245-255 JJ denotes homozygous
T9514 256-263 NN denotes patient
T9515 265-267 NN denotes S9
T9516 268-273 NN denotes cells
T9517 274-277 VB denotes are
T9518 278-283 NN denotes IB3-1
T9519 284-289 NN denotes cells
T9520 290-294 WDT denotes that
T9521 295-299 VB denotes have
T9522 300-304 VB denotes been
T9523 305-316 VB denotes transfected
T9524 317-321 IN denotes with
T9525 322-326 NN denotes CFTR
T9526 327-332 VB denotes using
T9527 333-335 DT denotes an
T9528 336-352 JJ denotes adeno-associated
T9529 353-358 JJ denotes viral
T9530 359-365 NN denotes vector
T9531 366-369 CC denotes and
T9532 370-376 NN denotes NuLi-1
T9533 377-382 NN denotes cells
T9534 383-387 VB denotes were
T9535 388-395 VB denotes derived
T9536 396-400 IN denotes from
T9537 401-402 DT denotes a
T9538 403-410 NN denotes patient
T9539 411-421 VB denotes possessing
T9540 422-423 DT denotes a
T9541 424-433 JJ denotes wild-type
T9542 434-438 NN denotes CFTR
T9543 439-447 NN denotes genotype
T9544 449-459 JJ denotes THP1-XBlue
T9545 460-465 NN denotes cells
T9546 466-472 RB denotes stably
T9547 473-480 VB denotes express
T9548 481-482 DT denotes a
T9549 483-491 VB denotes secreted
T9550 492-501 JJ denotes embryonic
T9551 502-510 NN denotes alkaline
T9552 511-522 NN denotes phosphatase
T9553 523-524 -LRB- denotes (
T9554 524-528 NN denotes SEAP
T9555 528-529 -RRB- denotes )
T9556 530-538 NN denotes reporter
T9557 539-548 JJ denotes inducible
T9558 549-551 IN denotes by
T9559 552-557 NN denotes NF-κB
T9560 558-561 CC denotes and
T9561 562-566 NN denotes AP-1
T9562 567-568 -LRB- denotes (
T9563 568-577 NN denotes Invivogen
T9564 577-578 -RRB- denotes )
T9565 580-585 NN denotes Cells
T9566 586-590 VB denotes were
T9567 591-599 VB denotes cultured
T9568 600-602 IN denotes as
T9569 603-614 VB denotes recommended
T9570 615-617 IN denotes by
T9571 618-623 PRP-DOLLAR- denotes their
T9572 624-634 JJ denotes respective
T9573 635-644 NN denotes suppliers
T9574 645-650 VB denotes using
T9575 651-659 JJ denotes standard
T9576 660-669 NN denotes protocols
T9577 671-673 NN denotes S9
T9578 674-677 CC denotes and
T9579 678-683 NN denotes IB3-1
T9580 684-689 NN denotes cells
T9581 690-694 VB denotes were
T9582 695-703 VB denotes cultured
T9583 704-706 IN denotes in
T9584 707-712 JJ denotes basal
T9585 713-718 NN denotes LHC-8
T9586 719-720 -LRB- denotes (
T9587 720-730 NN denotes Invitrogen
T9588 730-731 -RRB- denotes )
T9589 732-744 VB denotes supplemented
T9590 745-749 IN denotes with
T9591 750-752 CD denotes 10
T9592 752-753 NN denotes %
T9593 754-755 -LRB- denotes (
T9594 755-758 NN denotes v/v
T9595 758-759 -RRB- denotes )
T9596 760-765 JJ denotes fetal
T9597 766-772 JJ denotes bovine
T9598 773-778 NN denotes serum
T9599 779-780 -LRB- denotes (
T9600 780-783 NN denotes FBS
T9601 783-784 -RRB- denotes )
T9602 784-785 -COMMA- denotes ,
T9603 786-787 CD denotes 2
T9604 788-790 NN denotes mM
T9605 791-802 NN denotes L-glutamine
T9606 802-803 -COMMA- denotes ,
T9607 804-807 CC denotes and
T9608 808-809 CD denotes 1
T9609 810-812 NN denotes mM
T9610 813-819 NN denotes sodium
T9611 820-828 NN denotes pyruvate
T9612 830-843 NN denotes NuLi-1/CuFi-1
T9613 844-849 NN denotes cells
T9614 850-854 VB denotes were
T9615 855-863 VB denotes cultured
T9616 864-866 IN denotes in
T9617 867-871 NN denotes BEBM
T9618 872-882 JJ denotes serum-free
T9619 883-889 NN denotes medium
T9620 890-891 -LRB- denotes (
T9621 891-896 NN denotes Lonza
T9622 896-897 -RRB- denotes )
T9623 898-902 IN denotes with
T9624 903-913 NN denotes supplement
T9625 914-920 NN denotes bullet
T9626 921-924 NN denotes kit
T9627 925-926 -LRB- denotes (
T9628 926-929 NN denotes EGF
T9629 929-930 -COMMA- denotes ,
T9630 931-945 NN denotes hydrocortisone
T9631 945-946 -COMMA- denotes ,
T9632 947-953 JJ denotes bovine
T9633 954-963 JJ denotes pituitary
T9634 964-971 NN denotes extract
T9635 971-972 -COMMA- denotes ,
T9636 973-984 NN denotes transferrin
T9637 984-985 -COMMA- denotes ,
T9638 986-992 JJ denotes bovine
T9639 993-1000 NN denotes insulin
T9640 1000-1001 -COMMA- denotes ,
T9641 1002-1018 NN denotes triiodothyronine
T9642 1018-1019 -COMMA- denotes ,
T9643 1020-1031 NN denotes epinephrine
T9644 1031-1032 -COMMA- denotes ,
T9645 1033-1041 JJ denotes retinoic
T9646 1042-1046 NN denotes acid
T9647 1046-1047 -RRB- denotes )
T9648 1047-1048 -COMMA- denotes ,
T9649 1049-1050 CD denotes 2
T9650 1051-1053 NN denotes mM
T9651 1054-1065 NN denotes L-glutamine
T9652 1065-1066 -COMMA- denotes ,
T9653 1067-1070 CC denotes and
T9654 1071-1072 CD denotes 1
T9655 1073-1075 NN denotes mM
T9656 1076-1082 NN denotes sodium
T9657 1083-1091 NN denotes pyruvate
T9658 1093-1098 NN denotes PBMCs
T9659 1099-1103 IN denotes from
T9660 1104-1106 NN denotes CF
T9661 1107-1115 NN denotes patients
T9662 1116-1119 CC denotes and
T9663 1120-1128 NN denotes controls
T9664 1129-1133 VB denotes were
T9665 1134-1142 VB denotes cultured
T9666 1143-1145 IN denotes in
T9667 1146-1155 NN denotes RPMI-1640
T9668 1156-1157 -LRB- denotes (
T9669 1157-1164 NN denotes Hyclone
T9670 1164-1165 -RRB- denotes )
T9671 1166-1178 VB denotes supplemented
T9672 1179-1183 IN denotes with
T9673 1184-1186 CD denotes 10
T9674 1186-1187 NN denotes %
T9675 1188-1191 NNP denotes FBS
T9676 1191-1192 -COMMA- denotes ,
T9677 1193-1194 CD denotes 2
T9678 1195-1197 NN denotes mM
T9679 1198-1209 NN denotes L-glutamine
T9680 1209-1210 -COMMA- denotes ,
T9681 1211-1214 CC denotes and
T9682 1215-1216 CD denotes 1
T9683 1217-1219 NN denotes mM
T9684 1220-1226 NN denotes sodium
T9685 1227-1235 NN denotes pyruvate
T9686 1236-1237 -LRB- denotes (
T9687 1237-1245 JJ denotes complete
T9688 1246-1250 NN denotes RPMI
T9689 1250-1251 -RRB- denotes )
T9690 1253-1263 JJ denotes THP1-XBlue
T9691 1264-1269 NN denotes cells
T9692 1270-1274 VB denotes were
T9693 1275-1283 VB denotes cultured
T9694 1284-1286 IN denotes in
T9695 1287-1295 JJ denotes complete
T9696 1296-1300 NN denotes RPMI
T9697 1301-1305 IN denotes with
T9698 1306-1309 DT denotes the
T9699 1310-1318 NN denotes addition
T9700 1319-1321 IN denotes of
T9701 1322-1328 NN denotes zeocin
T9702 1329-1330 -LRB- denotes (
T9703 1330-1333 CD denotes 100
T9704 1334-1339 NN denotes µg/ml
T9705 1339-1340 -RRB- denotes )
T9706 1341-1343 TO denotes to
T9707 1344-1350 VB denotes select
T9708 1351-1354 IN denotes for
T9709 1355-1360 NN denotes cells
T9710 1361-1371 VB denotes expressing
T9711 1372-1375 DT denotes the
T9712 1376-1380 NN denotes SEAP
T9713 1381-1391 NN denotes NF-κB/AP-1
T9714 1392-1400 NN denotes reporter
T9715 1402-1407 RB denotes Prior
T9716 1408-1410 TO denotes to
T9717 1411-1422 NN denotes stimulation
T9718 1422-1423 -COMMA- denotes ,
T9719 1424-1433 JJ denotes bronchial
T9720 1434-1444 JJ denotes epithelial
T9721 1445-1449 NN denotes cell
T9722 1450-1455 NN denotes lines
T9723 1456-1460 VB denotes were
T9724 1461-1467 VB denotes plated
T9725 1468-1470 IN denotes in
T9726 1471-1477 VB denotes coated
T9727 1478-1479 -LRB- denotes [
T9728 1479-1481 CD denotes 62
T9729 1481-1482 -RRB- denotes ]
T9730 1483-1490 JJ denotes 96-well
T9731 1491-1497 NN denotes plates
T9732 1498-1499 -LRB- denotes (
T9733 1499-1501 NN denotes BD
T9734 1502-1513 NNP denotes Biosciences
T9735 1513-1514 -RRB- denotes )
T9736 1515-1517 IN denotes at
T9737 1518-1523 CD denotes 3×104
T9738 1524-1534 NN denotes cells/well
T9739 1535-1541 IN denotes unless
T9740 1542-1551 VB denotes indicated
T9741 1551-1552 -COMMA- denotes ,
T9742 1553-1556 CC denotes and
T9743 1557-1564 VB denotes allowed
T9744 1565-1567 TO denotes to
T9745 1568-1574 VB denotes adhere
T9746 1575-1584 RB denotes overnight
T9747 1586-1592 NN denotes Plates
T9748 1593-1596 IN denotes for
T9749 1597-1599 NN denotes S9
T9750 1600-1603 CC denotes and
T9751 1604-1609 NN denotes IB3-1
T9752 1610-1622 NN denotes stimulations
T9753 1623-1627 VB denotes were
T9754 1628-1634 VB denotes coated
T9755 1635-1637 IN denotes in
T9756 1638-1639 DT denotes a
T9757 1640-1647 NN denotes mixture
T9758 1648-1650 IN denotes of
T9759 1651-1657 JJ denotes bovine
T9760 1658-1663 NN denotes serum
T9761 1664-1671 NN denotes albumin
T9762 1672-1673 -LRB- denotes (
T9763 1673-1676 CD denotes 100
T9764 1677-1682 NN denotes µg/ml
T9765 1682-1683 -RRB- denotes )
T9766 1683-1684 -COMMA- denotes ,
T9767 1685-1696 NN denotes fibronectin
T9768 1697-1698 -LRB- denotes (
T9769 1698-1700 CD denotes 10
T9770 1701-1706 NN denotes µg/ml
T9771 1706-1707 -RRB- denotes )
T9772 1707-1708 -COMMA- denotes ,
T9773 1709-1712 CC denotes and
T9774 1713-1719 JJ denotes bovine
T9775 1720-1727 NN denotes collage
T9776 1728-1732 NN denotes type
T9777 1733-1734 CD denotes I
T9778 1735-1736 -LRB- denotes (
T9779 1736-1738 CD denotes 30
T9780 1739-1744 NN denotes µg/ml
T9781 1744-1745 -RRB- denotes )
T9782 1746-1747 -LRB- denotes (
T9783 1747-1749 NN denotes BD
T9784 1750-1761 NNP denotes Biosciences
T9785 1761-1762 -RRB- denotes )
T9786 1764-1770 NN denotes Plates
T9787 1771-1774 IN denotes for
T9788 1775-1781 NN denotes NuLi-1
T9789 1782-1785 CC denotes and
T9790 1786-1792 NN denotes CuFi-1
T9791 1793-1797 VB denotes were
T9792 1798-1804 VB denotes coated
T9793 1805-1809 IN denotes with
T9794 1810-1818 NN denotes collagen
T9795 1819-1823 NN denotes type
T9796 1824-1826 CD denotes IV
T9797 1827-1828 -LRB- denotes (
T9798 1828-1830 CD denotes 60
T9799 1831-1836 NN denotes µg/ml
T9800 1836-1837 -RRB- denotes )
T9801 1838-1839 -LRB- denotes (
T9802 1839-1844 NNP denotes Sigma
T9803 1845-1852 NNP denotes Aldrich
T9804 1852-1853 -RRB- denotes )
T9805 1855-1860 NN denotes PBMCs
T9806 1861-1865 VB denotes were
T9807 1866-1872 VB denotes plated
T9808 1873-1875 IN denotes in
T9809 1876-1883 JJ denotes 96-well
T9810 1884-1890 NN denotes plates
T9811 1891-1893 IN denotes at
T9812 1894-1895 DT denotes a
T9813 1896-1903 NN denotes density
T9814 1904-1906 IN denotes of
T9815 1907-1914 CD denotes 1.5×105
T9816 1915-1925 NN denotes cells/well
T9817 1926-1928 IN denotes in
T9818 1929-1932 CD denotes 200
T9819 1933-1935 NN denotes µl
T9820 1936-1937 -LRB- denotes (
T9821 1937-1944 CD denotes 7.5×105
T9822 1945-1953 NN denotes cells/mL
T9823 1953-1954 -RRB- denotes )
T9824 1956-1961 NN denotes THP-1
T9825 1962-1970 NN denotes reporter
T9826 1971-1976 NN denotes cells
T9827 1977-1981 VB denotes were
T9828 1982-1996 VB denotes differentiated
T9829 1997-2001 IN denotes into
T9830 2002-2003 DT denotes a
T9831 2004-2019 JJ denotes macrophage-like
T9832 2020-2029 NN denotes phenotype
T9833 2030-2035 VB denotes using
T9834 2036-2038 CD denotes 50
T9835 2039-2044 NN denotes ng/ml
T9836 2045-2047 IN denotes of
T9837 2048-2055 NN denotes phorbol
T9838 2056-2068 NN denotes 12-myristate
T9839 2069-2079 NN denotes 13-acetate
T9840 2080-2081 -LRB- denotes (
T9841 2081-2084 NN denotes PMA
T9842 2084-2085 -RRB- denotes )
T9843 2086-2087 -LRB- denotes (
T9844 2087-2092 NNP denotes Sigma
T9845 2093-2100 NNP denotes Aldrich
T9846 2100-2101 -RRB- denotes )
T9847 2102-2105 IN denotes for
T9848 2106-2108 CD denotes 24
T9849 2109-2114 NN denotes hours
T9850 2115-2117 IN denotes at
T9851 2118-2119 DT denotes a
T9852 2120-2127 NN denotes density
T9853 2128-2130 IN denotes of
T9854 2131-2136 CD denotes 1×105
T9855 2137-2147 NN denotes cells/well
T9856 2148-2150 IN denotes in
T9857 2151-2154 CD denotes 200
T9858 2155-2157 NN denotes µl
T9859 2158-2159 -LRB- denotes (
T9860 2159-2164 CD denotes 5×105
T9861 2165-2173 NN denotes cells/ml
T9862 2173-2174 -RRB- denotes )
T9863 2176-2181 NN denotes Cells
T9864 2182-2186 VB denotes were
T9865 2187-2193 VB denotes washed
T9866 2194-2198 IN denotes with
T9867 2199-2202 NNP denotes PBS
T9868 2203-2206 CC denotes and
T9869 2207-2214 VB denotes allowed
T9870 2215-2217 TO denotes to
T9871 2218-2222 VB denotes rest
T9872 2223-2224 DT denotes a
T9873 2225-2232 JJ denotes further
T9874 2233-2235 CD denotes 42
T9875 2236-2241 NN denotes hours
T9876 2242-2247 RB denotes prior
T9877 2248-2250 TO denotes to
T9878 2251-2262 NN denotes stimulation
R8253 T9470 T9469 arg1Of Culture,Cell
R8254 T9471 T9472 arg1Of CF,(
R8255 T9471 T9477 arg1Of CF,and
R8256 T9473 T9474 arg1Of IB3-1,and
R8257 T9474 T9472 arg2Of and,(
R8258 T9475 T9474 arg2Of CuFi-1,and
R8259 T9476 T9472 arg3Of ),(
R8260 T9477 T9495 arg1Of and,were
R8261 T9477 T9496 arg2Of and,derived
R8262 T9478 T9477 arg2Of control,and
R8263 T9478 T9479 arg1Of control,(
R8264 T9480 T9481 arg1Of S9,and
R8265 T9481 T9479 arg2Of and,(
R8266 T9482 T9481 arg2Of NuLi-1,and
R8267 T9483 T9479 arg3Of ),(
R8268 T9484 T9485 arg1Of cells,were
R8269 T9484 T9486 arg2Of cells,obtained
R8270 T9486 T9485 arg2Of obtained,were
R8271 T9486 T9487 arg1Of obtained,from
R8272 T9492 T9489 arg1Of Collection.,American
R8273 T9492 T9490 arg1Of Collection.,Type
R8274 T9492 T9491 arg1Of Collection.,Culture
R8275 T9494 T9487 arg2Of cells,from
R8276 T9494 T9488 arg1Of cells,the
R8277 T9494 T9492 arg1Of cells,Collection.
R8278 T9494 T9493 arg1Of cells,IB3-1
R8279 T9496 T9485 modOf derived,were
R8280 T9496 T9495 arg2Of derived,were
R8281 T9496 T9497 arg1Of derived,from
R8282 T9496 T9506 arg1Of derived,and
R8283 T9499 T9497 arg2Of patient,from
R8284 T9499 T9498 arg1Of patient,a
R8285 T9499 T9500 arg1Of patient,expressing
R8286 T9502 T9503 arg1Of ΔF508,and
R8287 T9504 T9503 arg2Of W1282X,and
R8288 T9505 T9500 arg2Of mutations,expressing
R8289 T9505 T9501 arg1Of mutations,the
R8290 T9505 T9502 arg1Of mutations,ΔF508
R8291 T9505 T9504 arg1Of mutations,W1282X
R8292 T9507 T9508 arg1Of CuFi-1,were
R8293 T9507 T9509 arg2Of CuFi-1,derived
R8294 T9509 T9506 arg2Of derived,and
R8295 T9509 T9508 arg2Of derived,were
R8296 T9509 T9510 arg1Of derived,from
R8297 T9514 T9510 arg2Of patient,from
R8298 T9514 T9511 arg1Of patient,a
R8299 T9514 T9512 arg1Of patient,ΔF508
R8300 T9514 T9513 arg1Of patient,homozygous
R8301 T9516 T9515 arg1Of cells,S9
R8302 T9516 T9517 arg1Of cells,are
R8303 T9517 T9531 arg1Of are,and
R8304 T9519 T9517 arg2Of cells,are
R8305 T9519 T9518 arg1Of cells,IB3-1
R8306 T9519 T9520 arg1Of cells,that
R8307 T9519 T9521 arg1Of cells,have
R8308 T9519 T9522 arg1Of cells,been
R8309 T9519 T9523 arg2Of cells,transfected
R8310 T9523 T9521 arg2Of transfected,have
R8311 T9523 T9522 arg2Of transfected,been
R8312 T9523 T9524 arg1Of transfected,with
R8313 T9525 T9524 arg2Of CFTR,with
R8314 T9525 T9526 arg1Of CFTR,using
R8315 T9530 T9526 arg2Of vector,using
R8316 T9530 T9527 arg1Of vector,an
R8317 T9530 T9528 arg1Of vector,adeno-associated
R8318 T9530 T9529 arg1Of vector,viral
R8319 T9533 T9532 arg1Of cells,NuLi-1
R8320 T9533 T9534 arg1Of cells,were
R8321 T9533 T9535 arg2Of cells,derived
R8322 T9535 T9531 arg2Of derived,and
R8323 T9535 T9534 arg2Of derived,were
R8324 T9535 T9536 arg1Of derived,from
R8325 T9538 T9536 arg2Of patient,from
R8326 T9538 T9537 arg1Of patient,a
R8327 T9538 T9539 arg1Of patient,possessing
R8328 T9543 T9539 arg2Of genotype,possessing
R8329 T9543 T9540 arg1Of genotype,a
R8330 T9543 T9541 arg1Of genotype,wild-type
R8331 T9543 T9542 arg1Of genotype,CFTR
R8332 T9545 T9544 arg1Of cells,THP1-XBlue
R8333 T9545 T9547 arg1Of cells,express
R8334 T9547 T9546 arg1Of express,stably
R8335 T9554 T9553 arg2Of SEAP,(
R8336 T9555 T9553 arg3Of ),(
R8337 T9556 T9547 arg2Of reporter,express
R8338 T9556 T9548 arg1Of reporter,a
R8339 T9556 T9549 arg2Of reporter,secreted
R8340 T9556 T9550 arg1Of reporter,embryonic
R8341 T9556 T9551 arg1Of reporter,alkaline
R8342 T9556 T9552 arg1Of reporter,phosphatase
R8343 T9556 T9553 arg1Of reporter,(
R8344 T9556 T9557 arg1Of reporter,inducible
R8345 T9557 T9558 arg1Of inducible,by
R8346 T9559 T9560 arg1Of NF-κB,and
R8347 T9560 T9558 arg2Of and,by
R8348 T9560 T9562 arg1Of and,(
R8349 T9561 T9560 arg2Of AP-1,and
R8350 T9563 T9562 arg2Of Invivogen,(
R8351 T9564 T9562 arg3Of ),(
R8352 T9565 T9566 arg1Of Cells,were
R8353 T9565 T9567 arg2Of Cells,cultured
R8354 T9567 T9566 arg2Of cultured,were
R8355 T9567 T9568 arg1Of cultured,as
R8356 T9567 T9574 modOf cultured,using
R8357 T9569 T9568 arg2Of recommended,as
R8358 T9573 T9569 arg1Of suppliers,recommended
R8359 T9573 T9570 arg2Of suppliers,by
R8360 T9573 T9571 arg1Of suppliers,their
R8361 T9573 T9572 arg1Of suppliers,respective
R8362 T9576 T9574 arg2Of protocols,using
R8363 T9576 T9575 arg1Of protocols,standard
R8364 T9577 T9578 arg1Of S9,and
R8365 T9579 T9578 arg2Of IB3-1,and
R8366 T9580 T9577 arg1Of cells,S9
R8367 T9580 T9579 arg1Of cells,IB3-1
R8368 T9580 T9581 arg1Of cells,were
R8369 T9580 T9582 arg2Of cells,cultured
R8370 T9582 T9581 arg2Of cultured,were
R8371 T9582 T9583 arg1Of cultured,in
R8372 T9585 T9584 arg1Of LHC-8,basal
R8373 T9585 T9586 arg1Of LHC-8,(
R8374 T9585 T9589 arg2Of LHC-8,supplemented
R8375 T9587 T9586 arg2Of Invitrogen,(
R8376 T9588 T9586 arg3Of ),(
R8377 T9589 T9590 arg1Of supplemented,with
R8378 T9592 T9590 arg2Of %,with
R8379 T9592 T9591 arg1Of %,10
R8380 T9594 T9593 arg2Of v/v,(
R8381 T9595 T9593 arg3Of ),(
R8382 T9598 T9585 arg1Of serum,LHC-8
R8383 T9598 T9593 arg1Of serum,(
R8384 T9598 T9596 arg1Of serum,fetal
R8385 T9598 T9597 arg1Of serum,bovine
R8386 T9598 T9599 arg1Of serum,(
R8387 T9598 T9602 arg1Of serum,","
R8388 T9600 T9599 arg2Of FBS,(
R8389 T9601 T9599 arg3Of ),(
R8390 T9602 T9607 arg1Of ",",and
R8391 T9603 T9604 arg1Of 2,mM
R8392 T9605 T9602 arg2Of L-glutamine,","
R8393 T9605 T9603 arg1Of L-glutamine,2
R8394 T9607 T9583 arg2Of and,in
R8395 T9607 T9606 arg1Of and,","
R8396 T9608 T9609 arg1Of 1,mM
R8397 T9611 T9607 arg2Of pyruvate,and
R8398 T9611 T9608 arg1Of pyruvate,1
R8399 T9611 T9610 arg1Of pyruvate,sodium
R8400 T9613 T9612 arg1Of cells,NuLi-1/CuFi-1
R8401 T9613 T9614 arg1Of cells,were
R8402 T9613 T9615 arg2Of cells,cultured
R8403 T9615 T9614 arg2Of cultured,were
R8404 T9615 T9616 arg1Of cultured,in
R8405 T9619 T9617 arg1Of medium,BEBM
R8406 T9619 T9618 arg1Of medium,serum-free
R8407 T9619 T9620 arg1Of medium,(
R8408 T9619 T9623 arg1Of medium,with
R8409 T9619 T9653 arg1Of medium,and
R8410 T9621 T9620 arg2Of Lonza,(
R8411 T9622 T9620 arg3Of ),(
R8412 T9626 T9624 arg1Of kit,supplement
R8413 T9626 T9625 arg1Of kit,bullet
R8414 T9626 T9627 arg1Of kit,(
R8415 T9626 T9648 arg1Of kit,","
R8416 T9628 T9629 arg1Of EGF,","
R8417 T9628 T9631 arg1Of EGF,","
R8418 T9628 T9635 arg1Of EGF,","
R8419 T9628 T9637 arg1Of EGF,","
R8420 T9630 T9629 arg2Of hydrocortisone,","
R8421 T9634 T9631 arg2Of extract,","
R8422 T9634 T9632 arg1Of extract,bovine
R8423 T9634 T9633 arg1Of extract,pituitary
R8424 T9636 T9635 arg2Of transferrin,","
R8425 T9637 T9627 arg2Of ",",(
R8426 T9637 T9640 arg1Of ",",","
R8427 T9639 T9637 arg2Of insulin,","
R8428 T9639 T9638 arg1Of insulin,bovine
R8429 T9641 T9640 arg2Of triiodothyronine,","
R8430 T9641 T9642 arg1Of triiodothyronine,","
R8431 T9641 T9644 arg1Of triiodothyronine,","
R8432 T9643 T9642 arg2Of epinephrine,","
R8433 T9646 T9644 arg2Of acid,","
R8434 T9646 T9645 arg1Of acid,retinoic
R8435 T9647 T9627 arg3Of ),(
R8436 T9648 T9623 arg2Of ",",with
R8437 T9649 T9650 arg1Of 2,mM
R8438 T9651 T9648 arg2Of L-glutamine,","
R8439 T9651 T9649 arg1Of L-glutamine,2
R8440 T9653 T9616 arg2Of and,in
R8441 T9653 T9652 arg1Of and,","
R8442 T9654 T9655 arg1Of 1,mM
R8443 T9657 T9653 arg2Of pyruvate,and
R8444 T9657 T9654 arg1Of pyruvate,1
R8445 T9657 T9656 arg1Of pyruvate,sodium
R8446 T9658 T9659 arg1Of PBMCs,from
R8447 T9658 T9664 arg1Of PBMCs,were
R8448 T9658 T9665 arg2Of PBMCs,cultured
R8449 T9661 T9660 arg1Of patients,CF
R8450 T9661 T9662 arg1Of patients,and
R8451 T9662 T9659 arg2Of and,from
R8452 T9663 T9662 arg2Of controls,and
R8453 T9665 T9664 arg2Of cultured,were
R8454 T9665 T9666 arg1Of cultured,in
R8455 T9667 T9668 arg1Of RPMI-1640,(
R8456 T9667 T9671 arg2Of RPMI-1640,supplemented
R8457 T9667 T9681 arg1Of RPMI-1640,and
R8458 T9669 T9668 arg2Of Hyclone,(
R8459 T9670 T9668 arg3Of ),(
R8460 T9671 T9672 arg1Of supplemented,with
R8461 T9674 T9673 arg1Of %,10
R8462 T9675 T9672 arg2Of FBS,with
R8463 T9675 T9674 arg1Of FBS,%
R8464 T9675 T9676 arg1Of FBS,","
R8465 T9677 T9678 arg1Of 2,mM
R8466 T9679 T9676 arg2Of L-glutamine,","
R8467 T9679 T9677 arg1Of L-glutamine,2
R8468 T9681 T9666 arg2Of and,in
R8469 T9681 T9680 arg1Of and,","
R8470 T9682 T9683 arg1Of 1,mM
R8471 T9685 T9681 arg2Of pyruvate,and
R8472 T9685 T9682 arg1Of pyruvate,1
R8473 T9685 T9684 arg1Of pyruvate,sodium
R8474 T9685 T9686 arg1Of pyruvate,(
R8475 T9688 T9686 arg2Of RPMI,(
R8476 T9688 T9687 arg1Of RPMI,complete
R8477 T9689 T9686 arg3Of ),(
R8478 T9691 T9690 arg1Of cells,THP1-XBlue
R8479 T9691 T9692 arg1Of cells,were
R8480 T9691 T9693 arg2Of cells,cultured
R8481 T9693 T9692 arg2Of cultured,were
R8482 T9693 T9694 arg1Of cultured,in
R8483 T9693 T9697 arg1Of cultured,with
R8484 T9696 T9694 arg2Of RPMI,in
R8485 T9696 T9695 arg1Of RPMI,complete
R8486 T9699 T9697 arg2Of addition,with
R8487 T9699 T9698 arg1Of addition,the
R8488 T9699 T9700 arg1Of addition,of
R8489 T9699 T9706 modOf addition,to
R8490 T9701 T9700 arg2Of zeocin,of
R8491 T9701 T9702 arg1Of zeocin,(
R8492 T9704 T9702 arg2Of µg/ml,(
R8493 T9704 T9703 arg1Of µg/ml,100
R8494 T9705 T9702 arg3Of ),(
R8495 T9707 T9706 arg1Of select,to
R8496 T9707 T9708 arg1Of select,for
R8497 T9709 T9708 arg2Of cells,for
R8498 T9709 T9710 arg1Of cells,expressing
R8499 T9714 T9710 arg2Of reporter,expressing
R8500 T9714 T9711 arg1Of reporter,the
R8501 T9714 T9712 arg1Of reporter,SEAP
R8502 T9714 T9713 arg1Of reporter,NF-κB/AP-1
R8503 T9715 T9716 arg1Of Prior,to
R8504 T9717 T9716 arg2Of stimulation,to
R8505 T9722 T9719 arg1Of lines,bronchial
R8506 T9722 T9720 arg1Of lines,epithelial
R8507 T9722 T9721 arg1Of lines,cell
R8508 T9722 T9723 arg1Of lines,were
R8509 T9722 T9724 arg2Of lines,plated
R8510 T9724 T9715 arg1Of plated,Prior
R8511 T9724 T9718 arg1Of plated,","
R8512 T9724 T9723 arg2Of plated,were
R8513 T9724 T9725 arg1Of plated,in
R8514 T9724 T9736 arg1Of plated,at
R8515 T9724 T9739 arg1Of plated,unless
R8516 T9728 T9727 arg2Of 62,[
R8517 T9729 T9727 arg3Of ],[
R8518 T9731 T9725 arg2Of plates,in
R8519 T9731 T9726 arg1Of plates,coated
R8520 T9731 T9727 arg1Of plates,[
R8521 T9731 T9730 arg1Of plates,96-well
R8522 T9731 T9732 arg1Of plates,(
R8523 T9733 T9732 arg2Of BD,(
R8524 T9733 T9734 arg1Of BD,Biosciences
R8525 T9735 T9732 arg3Of ),(
R8526 T9738 T9736 arg2Of cells/well,at
R8527 T9738 T9737 arg1Of cells/well,3×104
R8528 T9740 T9742 arg1Of indicated,and
R8529 T9742 T9739 arg2Of and,unless
R8530 T9742 T9741 arg1Of and,","
R8531 T9743 T9742 arg2Of allowed,and
R8532 T9745 T9743 arg3Of adhere,allowed
R8533 T9745 T9744 arg1Of adhere,to
R8534 T9745 T9746 arg1Of adhere,overnight
R8535 T9747 T9748 arg1Of Plates,for
R8536 T9747 T9753 arg1Of Plates,were
R8537 T9747 T9754 arg2Of Plates,coated
R8538 T9749 T9750 arg1Of S9,and
R8539 T9751 T9750 arg2Of IB3-1,and
R8540 T9752 T9748 arg2Of stimulations,for
R8541 T9752 T9749 arg1Of stimulations,S9
R8542 T9752 T9751 arg1Of stimulations,IB3-1
R8543 T9754 T9753 arg2Of coated,were
R8544 T9754 T9755 arg1Of coated,in
R8545 T9757 T9755 arg2Of mixture,in
R8546 T9757 T9756 arg1Of mixture,a
R8547 T9757 T9758 arg1Of mixture,of
R8548 T9761 T9759 arg1Of albumin,bovine
R8549 T9761 T9760 arg1Of albumin,serum
R8550 T9761 T9762 arg1Of albumin,(
R8551 T9761 T9766 arg1Of albumin,","
R8552 T9764 T9762 arg2Of µg/ml,(
R8553 T9764 T9763 arg1Of µg/ml,100
R8554 T9765 T9762 arg3Of ),(
R8555 T9766 T9773 arg1Of ",",and
R8556 T9767 T9766 arg2Of fibronectin,","
R8557 T9767 T9768 arg1Of fibronectin,(
R8558 T9770 T9768 arg2Of µg/ml,(
R8559 T9770 T9769 arg1Of µg/ml,10
R8560 T9771 T9768 arg3Of ),(
R8561 T9773 T9758 arg2Of and,of
R8562 T9773 T9772 arg1Of and,","
R8563 T9773 T9782 arg1Of and,(
R8564 T9776 T9773 arg2Of type,and
R8565 T9776 T9774 arg1Of type,bovine
R8566 T9776 T9775 arg1Of type,collage
R8567 T9776 T9777 arg1Of type,I
R8568 T9776 T9778 arg1Of type,(
R8569 T9780 T9778 arg2Of µg/ml,(
R8570 T9780 T9779 arg1Of µg/ml,30
R8571 T9781 T9778 arg3Of ),(
R8572 T9784 T9782 arg2Of Biosciences,(
R8573 T9784 T9783 arg1Of Biosciences,BD
R8574 T9785 T9782 arg3Of ),(
R8575 T9786 T9787 arg1Of Plates,for
R8576 T9786 T9791 arg1Of Plates,were
R8577 T9786 T9792 arg2Of Plates,coated
R8578 T9788 T9789 arg1Of NuLi-1,and
R8579 T9789 T9787 arg2Of and,for
R8580 T9790 T9789 arg2Of CuFi-1,and
R8581 T9792 T9791 arg2Of coated,were
R8582 T9792 T9793 arg1Of coated,with
R8583 T9795 T9793 arg2Of type,with
R8584 T9795 T9794 arg1Of type,collagen
R8585 T9795 T9796 arg1Of type,IV
R8586 T9795 T9797 arg1Of type,(
R8587 T9795 T9801 arg1Of type,(
R8588 T9799 T9797 arg2Of µg/ml,(
R8589 T9799 T9798 arg1Of µg/ml,60
R8590 T9800 T9797 arg3Of ),(
R8591 T9803 T9801 arg2Of Aldrich,(
R8592 T9803 T9802 arg1Of Aldrich,Sigma
R8593 T9804 T9801 arg3Of ),(
R8594 T9805 T9806 arg1Of PBMCs,were
R8595 T9805 T9807 arg2Of PBMCs,plated
R8596 T9807 T9806 arg2Of plated,were
R8597 T9807 T9808 arg1Of plated,in
R8598 T9807 T9811 arg1Of plated,at
R8599 T9810 T9808 arg2Of plates,in
R8600 T9810 T9809 arg1Of plates,96-well
R8601 T9813 T9811 arg2Of density,at
R8602 T9813 T9812 arg1Of density,a
R8603 T9813 T9814 arg1Of density,of
R8604 T9813 T9817 arg1Of density,in
R8605 T9816 T9814 arg2Of cells/well,of
R8606 T9816 T9815 arg1Of cells/well,1.5×105
R8607 T9819 T9817 arg2Of µl,in
R8608 T9819 T9818 arg1Of µl,200
R8609 T9819 T9820 arg1Of µl,(
R8610 T9822 T9820 arg2Of cells/mL,(
R8611 T9822 T9821 arg1Of cells/mL,7.5×105
R8612 T9823 T9820 arg3Of ),(
R8613 T9826 T9824 arg1Of cells,THP-1
R8614 T9826 T9825 arg1Of cells,reporter
R8615 T9826 T9827 arg1Of cells,were
R8616 T9826 T9828 arg2Of cells,differentiated
R8617 T9828 T9827 arg2Of differentiated,were
R8618 T9828 T9829 arg1Of differentiated,into
R8619 T9832 T9829 arg2Of phenotype,into
R8620 T9832 T9830 arg1Of phenotype,a
R8621 T9832 T9831 arg1Of phenotype,macrophage-like
R8622 T9832 T9833 arg1Of phenotype,using
R8623 T9833 T9847 arg1Of using,for
R8624 T9835 T9833 arg2Of ng/ml,using
R8625 T9835 T9834 arg1Of ng/ml,50
R8626 T9835 T9836 arg1Of ng/ml,of
R8627 T9839 T9836 arg2Of 13-acetate,of
R8628 T9839 T9837 arg1Of 13-acetate,phorbol
R8629 T9839 T9838 arg1Of 13-acetate,12-myristate
R8630 T9839 T9840 arg1Of 13-acetate,(
R8631 T9839 T9843 arg1Of 13-acetate,(
R8632 T9841 T9840 arg2Of PMA,(
R8633 T9842 T9840 arg3Of ),(
R8634 T9845 T9843 arg2Of Aldrich,(
R8635 T9845 T9844 arg1Of Aldrich,Sigma
R8636 T9846 T9843 arg3Of ),(
R8637 T9849 T9847 arg2Of hours,for
R8638 T9849 T9848 arg1Of hours,24
R8639 T9849 T9850 arg1Of hours,at
R8640 T9852 T9850 arg2Of density,at
R8641 T9852 T9851 arg1Of density,a
R8642 T9852 T9853 arg1Of density,of
R8643 T9852 T9856 arg1Of density,in
R8644 T9855 T9853 arg2Of cells/well,of
R8645 T9855 T9854 arg1Of cells/well,1×105
R8646 T9858 T9856 arg2Of µl,in
R8647 T9858 T9857 arg1Of µl,200
R8648 T9858 T9859 arg1Of µl,(
R8649 T9861 T9859 arg2Of cells/ml,(
R8650 T9861 T9860 arg1Of cells/ml,5×105
R8651 T9862 T9859 arg3Of ),(
R8652 T9863 T9864 arg1Of Cells,were
R8653 T9863 T9865 arg2Of Cells,washed
R8654 T9863 T9869 arg2Of Cells,allowed
R8655 T9863 T9871 arg1Of Cells,rest
R8656 T9865 T9866 arg1Of washed,with
R8657 T9865 T9868 arg1Of washed,and
R8658 T9867 T9866 arg2Of PBS,with
R8659 T9868 T9864 arg2Of and,were
R8660 T9869 T9868 arg2Of allowed,and
R8661 T9871 T9869 arg3Of rest,allowed
R8662 T9871 T9870 arg1Of rest,to
R8663 T9871 T9876 arg1Of rest,prior
R8664 T9875 T9871 arg2Of hours,rest
R8665 T9875 T9872 arg1Of hours,a
R8666 T9875 T9873 arg1Of hours,further
R8667 T9875 T9874 arg1Of hours,42
R8668 T9876 T9877 arg1Of prior,to
R8669 T9878 T9877 arg2Of stimulation,to

sentences

Id Subject Object Predicate Lexical cue
T9454 0-12 Sentence denotes Cell Culture
T9455 13-264 Sentence denotes CF (IB3-1 and CuFi-1) and control (S9 and NuLi-1) cells were obtained from the American Type Culture Collection. IB3-1 cells were derived from a patient expressing the ΔF508 and W1282X mutations and CuFi-1 were derived from a ΔF508 homozygous patient.
T9456 265-448 Sentence denotes S9 cells are IB3-1 cells that have been transfected with CFTR using an adeno-associated viral vector and NuLi-1 cells were derived from a patient possessing a wild-type CFTR genotype.
T9457 449-579 Sentence denotes THP1-XBlue cells stably express a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB and AP-1 (Invivogen).
T9458 580-670 Sentence denotes Cells were cultured as recommended by their respective suppliers using standard protocols.
T9459 671-829 Sentence denotes S9 and IB3-1 cells were cultured in basal LHC-8 (Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T9460 830-1092 Sentence denotes NuLi-1/CuFi-1 cells were cultured in BEBM serum-free medium (Lonza) with supplement bullet kit (EGF, hydrocortisone, bovine pituitary extract, transferrin, bovine insulin, triiodothyronine, epinephrine, retinoic acid), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T9461 1093-1252 Sentence denotes PBMCs from CF patients and controls were cultured in RPMI-1640 (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, and 1 mM sodium pyruvate (complete RPMI).
T9462 1253-1401 Sentence denotes THP1-XBlue cells were cultured in complete RPMI with the addition of zeocin (100 µg/ml) to select for cells expressing the SEAP NF-κB/AP-1 reporter.
T9463 1402-1585 Sentence denotes Prior to stimulation, bronchial epithelial cell lines were plated in coated [62] 96-well plates (BD Biosciences) at 3×104 cells/well unless indicated, and allowed to adhere overnight.
T9464 1586-1763 Sentence denotes Plates for S9 and IB3-1 stimulations were coated in a mixture of bovine serum albumin (100 µg/ml), fibronectin (10 µg/ml), and bovine collage type I (30 µg/ml) (BD Biosciences).
T9465 1764-1854 Sentence denotes Plates for NuLi-1 and CuFi-1 were coated with collagen type IV (60 µg/ml) (Sigma Aldrich).
T9466 1855-1955 Sentence denotes PBMCs were plated in 96-well plates at a density of 1.5×105 cells/well in 200 µl (7.5×105 cells/mL).
T9467 1956-2175 Sentence denotes THP-1 reporter cells were differentiated into a macrophage-like phenotype using 50 ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich) for 24 hours at a density of 1×105 cells/well in 200 µl (5×105 cells/ml).
T9468 2176-2263 Sentence denotes Cells were washed with PBS and allowed to rest a further 42 hours prior to stimulation.
T172 0-12 Sentence denotes Cell Culture
T173 13-125 Sentence denotes CF (IB3-1 and CuFi-1) and control (S9 and NuLi-1) cells were obtained from the American Type Culture Collection.
T174 126-264 Sentence denotes IB3-1 cells were derived from a patient expressing the ΔF508 and W1282X mutations and CuFi-1 were derived from a ΔF508 homozygous patient.
T175 265-448 Sentence denotes S9 cells are IB3-1 cells that have been transfected with CFTR using an adeno-associated viral vector and NuLi-1 cells were derived from a patient possessing a wild-type CFTR genotype.
T176 449-579 Sentence denotes THP1-XBlue cells stably express a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB and AP-1 (Invivogen).
T177 580-670 Sentence denotes Cells were cultured as recommended by their respective suppliers using standard protocols.
T178 671-829 Sentence denotes S9 and IB3-1 cells were cultured in basal LHC-8 (Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T179 830-1092 Sentence denotes NuLi-1/CuFi-1 cells were cultured in BEBM serum-free medium (Lonza) with supplement bullet kit (EGF, hydrocortisone, bovine pituitary extract, transferrin, bovine insulin, triiodothyronine, epinephrine, retinoic acid), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T180 1093-1252 Sentence denotes PBMCs from CF patients and controls were cultured in RPMI-1640 (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, and 1 mM sodium pyruvate (complete RPMI).
T181 1253-1401 Sentence denotes THP1-XBlue cells were cultured in complete RPMI with the addition of zeocin (100 µg/ml) to select for cells expressing the SEAP NF-κB/AP-1 reporter.
T182 1402-1585 Sentence denotes Prior to stimulation, bronchial epithelial cell lines were plated in coated [62] 96-well plates (BD Biosciences) at 3×104 cells/well unless indicated, and allowed to adhere overnight.
T183 1586-1763 Sentence denotes Plates for S9 and IB3-1 stimulations were coated in a mixture of bovine serum albumin (100 µg/ml), fibronectin (10 µg/ml), and bovine collage type I (30 µg/ml) (BD Biosciences).
T184 1764-1854 Sentence denotes Plates for NuLi-1 and CuFi-1 were coated with collagen type IV (60 µg/ml) (Sigma Aldrich).
T185 1855-1955 Sentence denotes PBMCs were plated in 96-well plates at a density of 1.5×105 cells/well in 200 µl (7.5×105 cells/mL).
T186 1956-2175 Sentence denotes THP-1 reporter cells were differentiated into a macrophage-like phenotype using 50 ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich) for 24 hours at a density of 1×105 cells/well in 200 µl (5×105 cells/ml).
T187 2176-2263 Sentence denotes Cells were washed with PBS and allowed to rest a further 42 hours prior to stimulation.

2_test

Id Subject Object Predicate Lexical cue
22649552-21068401-88306746 1479-1481 21068401 denotes 62
T53749 1479-1481 21068401 denotes 62