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PMC:3359311 / 25535-34177 JSONTXT

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test2

Id Subject Object Predicate Lexical cue
T9445 1369-1380 Protein denotes transferrin
T9446 1389-1396 Protein denotes insulin
T9447 2054-2067 Protein denotes serum albumin
T11752 7126-7130 Protein denotes CD14
T12138 7421-7441 Protein denotes alkaline phosphatase

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T9879 958-962 P05412 denotes AP-1
T9880 1783-1787 P05412 denotes AP-1
T11550 5746-5751 P01584 denotes IL-1β
T11551 5766-5770 P25963 denotes IκBα
T11552 5792-5799 P60709 denotes β-actin
T11927 7009-7013 P08571 denotes CD14
T11928 7126-7130 P08571 denotes CD14
T12207 7223-7227 P05412 denotes AP-1
T12208 7477-7481 P05412 denotes AP-1

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T9451 1369-1380 Protein denotes transferrin
T9452 1389-1396 Protein denotes insulin
T9453 2054-2067 Protein denotes serum albumin
T11378 5766-5770 Protein denotes IκBα
T11379 5792-5799 Protein denotes β-actin
T11754 7126-7130 Protein denotes CD14
T12139 7421-7441 Protein denotes alkaline phosphatase

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T10375 577-582 Protein denotes ΔF508
T10376 587-593 Protein denotes W1282X
T10377 562-572 Gene_expression denotes expressing
T10378 562-572 Gene_expression denotes expressing
T10379 718-722 Protein denotes CFTR
T10380 830-843 Protein denotes CFTR genotype
T10381 845-849 Protein denotes THP1
T10382 888-918 Protein denotes embryonic alkaline phosphatase
T10383 920-924 Protein denotes SEAP
T10384 948-953 Protein denotes NF-κB
T10385 958-974 Protein denotes AP-1 (Invivogen)
T10386 869-876 Gene_expression denotes express
T10387 869-876 Gene_expression denotes express
T10388 879-887 Localization denotes secreted
T10389 879-887 Localization denotes secreted
T10390 935-944 Positive_regulation denotes inducible
T10391 935-944 Positive_regulation denotes inducible
T10392 1299-1320 Protein denotes supplement bullet kit
T10393 1322-1325 Protein denotes EGF
T10394 1369-1380 Protein denotes transferrin
T10395 1382-1396 Protein denotes bovine insulin
T10396 1649-1653 Protein denotes THP1
T10397 1692-1696 Protein denotes RPMI
T10398 1772-1779 Protein denotes SEAP NF
T10399 1780-1782 Protein denotes κB
T10400 1783-1796 Protein denotes AP-1 reporter
T10401 1757-1767 Gene_expression denotes expressing
T10402 1757-1767 Gene_expression denotes expressing
T10403 1757-1767 Gene_expression denotes expressing
T10404 1993-1995 Protein denotes S9
T10405 2000-2005 Protein denotes IB3-1
T10406 2047-2067 Protein denotes bovine serum albumin
T10407 2081-2092 Protein denotes fibronectin
T10408 2206-2222 Protein denotes collagen type IV
T10860 3565-3578 Protein denotes complete RPMI
T11369 4327-4331 Protein denotes PAO1
T11370 4403-4407 Protein denotes PAO1
T11371 4676-4681 Protein denotes NF-κB
T11372 4856-4881 Protein denotes CFTR inhibitor CFTRinh172
T11373 4883-4896 Protein denotes Sigma Aldrich
T11374 4968-4977 Protein denotes caspase-1
T11748 5391-5420 Protein denotes Halt protease and phosphatase
T11749 5766-5799 Protein denotes IκBα (Cell Signaling), or β-actin
T11750 6061-6074 Protein denotes serum albumin
T12128 6262-6271 Protein denotes caspase-1
T12129 6466-6470 Protein denotes PAO1
T12130 6749-6753 Protein denotes PAO1
T12131 6767-6776 Protein denotes Caspase-1
T12132 6911-6920 Protein denotes caspase-1
T12133 6898-6903 Binding denotes binds
T12134 7009-7013 Protein denotes CD14
T12135 7126-7130 Protein denotes CD14
T12136 7131-7139 Gene_expression denotes positive
T12293 7217-7222 Protein denotes NF-κB
T12294 7223-7227 Protein denotes AP-1
T12295 7421-7441 Protein denotes alkaline phosphatase
T12296 7471-7476 Protein denotes NF-κB
T12297 7477-7481 Protein denotes AP-1
T12602 8052-8056 Protein denotes PAO1
T12603 8163-8167 Protein denotes PAO1
T12604 8341-8345 Protein denotes PAO1
T12605 8424-8428 Protein denotes PAO1
R9119 T10375 T10377 themeOf ΔF508,expressing
R9120 T10376 T10378 themeOf W1282X,expressing
R9121 T10382 T10386 themeOf embryonic alkaline phosphatase,express
R9122 T10382 T10388 themeOf embryonic alkaline phosphatase,secreted
R9123 T10382 T10390 themeOf embryonic alkaline phosphatase,inducible
R9124 T10382 T10391 themeOf embryonic alkaline phosphatase,inducible
R9125 T10383 T10387 themeOf SEAP,express
R9126 T10383 T10389 themeOf SEAP,secreted
R9127 T10384 T10390 causeOf NF-κB,inducible
R9128 T10385 T10391 causeOf AP-1 (Invivogen),inducible
R9129 T10398 T10401 themeOf SEAP NF,expressing
R9130 T10399 T10402 themeOf κB,expressing
R9131 T10400 T10403 themeOf AP-1 reporter,expressing
R10742 T12132 T12133 themeOf caspase-1,binds
R10743 T12135 T12136 themeOf CD14,positive

events-check-again

Id Subject Object Predicate Lexical cue
T10372 1369-1380 Protein denotes transferrin
T10373 1389-1396 Protein denotes insulin
T10374 2054-2067 Protein denotes serum albumin
T11746 5766-5770 Protein denotes IκBα
T11747 5792-5799 Protein denotes β-actin
T12127 7126-7130 Protein denotes CD14
T12292 7421-7441 Protein denotes alkaline phosphatase

GO-CC

Id Subject Object Predicate Lexical cue
T10347 396-400 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T10348 459-464 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10349 528-533 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10350 664-669 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10351 680-685 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10352 773-778 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10353 856-861 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10354 1080-1085 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10355 1240-1245 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10356 1660-1665 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10357 1751-1756 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10358 1920-1925 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10359 2311-2316 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10360 2341-2346 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10361 2367-2372 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10362 2533-2538 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10363 2561-2566 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10858 3656-3660 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T10859 3823-3828 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11364 3947-3951 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T11365 4013-4018 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11366 4106-4111 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11367 4256-4261 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11368 4371-4376 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11736 5286-5291 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11737 5637-5646 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T11738 5886-5896 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T11739 5886-5896 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T12116 6302-6307 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12117 6346-6351 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12118 6599-6604 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12119 6619-6624 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12120 7110-7115 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12121 7209-7214 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12122 7014-7024 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T12123 7014-7024 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T12289 7276-7281 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T10364 907-918 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T10365 1322-1325 http://purl.obolibrary.org/obo/GO_0005154 denotes EGF
T11740 5409-5420 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T11741 5886-5896 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T12124 7014-7024 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T12288 7430-7441 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-BP

Id Subject Object Predicate Lexical cue
T9441 256-259 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T10345 907-918 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T10346 2378-2410 http://purl.obolibrary.org/obo/GO_0030225 denotes differentiated into a macrophage
T10856 3169-3191 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory responses
T10857 3264-3267 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T11735 5409-5420 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T12287 7430-7441 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

UBERON-AE

Id Subject Object Predicate Lexical cue
T9308 40-45 http://purl.obolibrary.org/obo/UBERON_0000178 denotes Blood
T9448 1169-1174 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T9449 1268-1273 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T9450 2054-2059 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T10409 2766-2771 http://purl.obolibrary.org/obo/UBERON_0001089 denotes sweat
T10410 2909-2914 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T10411 3323-3328 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T10412 3361-3366 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T10413 3927-3932 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T11377 6061-6066 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T11753 6522-6527 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T9373 23-29 NNS denotes Ethics
T9374 30-39 NNP denotes Statement
T9375 40-45 NNP denotes Blood
T9376 46-53 NNS denotes samples
T9377 54-58 VBD denotes were
T9378 59-67 VBN denotes obtained
T9379 68-72 IN denotes with
T9380 73-81 JJ denotes informed
T9381 82-89 VBN denotes written
T9382 90-97 NN denotes consent
T9383 98-102 IN denotes from
T9384 103-110 NN denotes control
T9385 111-119 NNS denotes subjects
T9386 120-123 CC denotes and
T9387 124-126 NNP denotes CF
T9388 127-135 NNS denotes patients
T9389 136-138 IN denotes at
T9390 139-142 DT denotes the
T9391 143-145 NNP denotes BC
T9392 146-154 NNP denotes Children
T9393 154-156 POS denotes 's
T9394 157-165 NN denotes Hospital
T9395 165-166 . denotes .
T9396 167-174 NNP denotes Consent
T9397 175-178 VBD denotes was
T9398 179-187 VBN denotes obtained
T9399 188-191 IN denotes for
T9400 192-200 NNS denotes children
T9401 201-203 IN denotes by
T9402 204-209 PRP$ denotes their
T9403 210-216 NN denotes parent
T9404 217-219 CC denotes or
T9405 220-225 JJ denotes legal
T9406 226-234 NN denotes guardian
T9407 234-235 . denotes .
T9408 236-244 NNS denotes Subjects
T9409 245-246 CD denotes 7
T9410 247-252 NNS denotes years
T9411 253-255 IN denotes of
T9412 256-259 NN denotes age
T9413 260-263 CC denotes and
T9414 264-269 JJR denotes older
T9415 270-274 VBD denotes were
T9416 275-283 VBN denotes required
T9417 284-286 TO denotes to
T9418 287-294 VB denotes provide
T9419 295-303 JJ denotes informed
T9420 304-310 NN denotes assent
T9421 311-313 RB denotes as
T9422 314-318 RB denotes well
T9423 318-319 . denotes .
T9424 320-329 NNS denotes Protocols
T9425 330-334 VBD denotes were
T9426 335-343 VBN denotes approved
T9427 344-346 IN denotes by
T9428 347-350 DT denotes the
T9429 351-359 NNP denotes Clinical
T9430 360-368 NNP denotes Research
T9431 369-375 NNP denotes Ethics
T9432 376-381 NNP denotes Board
T9433 382-383 -LRB- denotes (
T9434 383-392 NNP denotes H09-01192
T9435 392-393 -RRB- denotes )
T9436 393-394 . denotes .
T9881 396-400 NNP denotes Cell
T9882 401-408 NNP denotes Culture
T9883 409-411 NNP denotes CF
T9884 412-413 -LRB- denotes (
T9885 413-418 NNP denotes IB3-1
T9886 419-422 CC denotes and
T9887 423-429 NNP denotes CuFi-1
T9888 429-430 -RRB- denotes )
T9889 431-434 CC denotes and
T9890 435-442 NN denotes control
T9891 443-444 -LRB- denotes (
T9892 444-446 CD denotes S9
T9893 447-450 CC denotes and
T9894 451-457 NNP denotes NuLi-1
T9895 457-458 -RRB- denotes )
T9896 459-464 NNS denotes cells
T9897 465-469 VBD denotes were
T9898 470-478 VBN denotes obtained
T9899 479-483 IN denotes from
T9900 484-487 DT denotes the
T9901 488-496 JJ denotes American
T9902 497-501 NNP denotes Type
T9903 502-509 NNP denotes Culture
T9904 510-520 NNP denotes Collection
T9905 520-521 . denotes .
T9906 522-527 CD denotes IB3-1
T9907 528-533 NNS denotes cells
T9908 534-538 VBD denotes were
T9909 539-546 VBN denotes derived
T9910 547-551 IN denotes from
T9911 552-553 DT denotes a
T9912 554-561 JJ denotes patient
T9913 562-572 VBG denotes expressing
T9914 573-576 DT denotes the
T9915 577-582 NNP denotes ΔF508
T9916 583-586 CC denotes and
T9917 587-593 NNP denotes W1282X
T9918 594-603 NNS denotes mutations
T9919 604-607 CC denotes and
T9920 608-614 NN denotes CuFi-1
T9921 615-619 VBD denotes were
T9922 620-627 VBN denotes derived
T9923 628-632 IN denotes from
T9924 633-634 DT denotes a
T9925 635-640 NNP denotes ΔF508
T9926 641-651 JJ denotes homozygous
T9927 652-659 NN denotes patient
T9928 659-660 . denotes .
T9929 661-663 CD denotes S9
T9930 664-669 NNS denotes cells
T9931 670-673 VBP denotes are
T9932 674-679 JJ denotes IB3-1
T9933 680-685 NNS denotes cells
T9934 686-690 WDT denotes that
T9935 691-695 VBP denotes have
T9936 696-700 VBN denotes been
T9937 701-712 VBN denotes transfected
T9938 713-717 IN denotes with
T9939 718-722 NNP denotes CFTR
T9940 723-728 VBG denotes using
T9941 729-731 DT denotes an
T9942 732-748 JJ denotes adeno-associated
T9943 749-754 JJ denotes viral
T9944 755-761 NN denotes vector
T9945 762-765 CC denotes and
T9946 766-772 JJ denotes NuLi-1
T9947 773-778 NNS denotes cells
T9948 779-783 VBD denotes were
T9949 784-791 VBN denotes derived
T9950 792-796 IN denotes from
T9951 797-798 DT denotes a
T9952 799-806 JJ denotes patient
T9953 807-817 VBG denotes possessing
T9954 818-819 DT denotes a
T9955 820-829 JJ denotes wild-type
T9956 830-834 NNP denotes CFTR
T9957 835-843 NN denotes genotype
T9958 843-844 . denotes .
T9959 845-855 JJ denotes THP1-XBlue
T9960 856-861 NNS denotes cells
T9961 862-868 RB denotes stably
T9962 869-876 VBP denotes express
T9963 877-878 DT denotes a
T9964 879-887 JJ denotes secreted
T9965 888-897 JJ denotes embryonic
T9966 898-906 NN denotes alkaline
T9967 907-918 NN denotes phosphatase
T9968 919-920 -LRB- denotes (
T9969 920-924 NNP denotes SEAP
T9970 924-925 -RRB- denotes )
T9971 926-934 NN denotes reporter
T9972 935-944 JJ denotes inducible
T9973 945-947 IN denotes by
T9974 948-953 NNP denotes NF-κB
T9975 954-957 CC denotes and
T9976 958-962 NNP denotes AP-1
T9977 963-964 -LRB- denotes (
T9978 964-973 NNP denotes Invivogen
T9979 973-974 -RRB- denotes )
T9980 974-975 . denotes .
T9981 976-981 NNS denotes Cells
T9982 982-986 VBD denotes were
T9983 987-995 VBN denotes cultured
T9984 996-998 IN denotes as
T9985 999-1010 VBN denotes recommended
T9986 1011-1013 IN denotes by
T9987 1014-1019 PRP$ denotes their
T9988 1020-1030 JJ denotes respective
T9989 1031-1040 NNS denotes suppliers
T9990 1041-1046 VBG denotes using
T9991 1047-1055 JJ denotes standard
T9992 1056-1065 NNS denotes protocols
T9993 1065-1066 . denotes .
T9994 1067-1069 CD denotes S9
T9995 1070-1073 CC denotes and
T9996 1074-1079 CD denotes IB3-1
T9997 1080-1085 NNS denotes cells
T9998 1086-1090 VBD denotes were
T9999 1091-1099 VBN denotes cultured
T10000 1100-1102 IN denotes in
T10001 1103-1108 JJ denotes basal
T10002 1109-1114 NN denotes LHC-8
T10003 1115-1116 -LRB- denotes (
T10004 1116-1126 NNP denotes Invitrogen
T10005 1126-1127 -RRB- denotes )
T10006 1128-1140 VBN denotes supplemented
T10007 1141-1145 IN denotes with
T10008 1146-1148 CD denotes 10
T10009 1148-1149 NN denotes %
T10010 1150-1151 -LRB- denotes (
T10011 1151-1154 FW denotes v/v
T10012 1154-1155 -RRB- denotes )
T10013 1156-1161 JJ denotes fetal
T10014 1162-1168 JJ denotes bovine
T10015 1169-1174 NN denotes serum
T10016 1175-1176 -LRB- denotes (
T10017 1176-1179 NNP denotes FBS
T10018 1179-1180 -RRB- denotes )
T10019 1180-1181 , denotes ,
T10020 1182-1183 CD denotes 2
T10021 1184-1186 NNP denotes mM
T10022 1187-1198 NNP denotes L-glutamine
T10023 1198-1199 , denotes ,
T10024 1200-1203 CC denotes and
T10025 1204-1205 CD denotes 1
T10026 1206-1208 NNP denotes mM
T10027 1209-1215 NN denotes sodium
T10028 1216-1224 NN denotes pyruvate
T10029 1224-1225 . denotes .
T10030 1226-1232 JJ denotes NuLi-1
T10031 1232-1233 NN denotes /
T10032 1233-1239 NN denotes CuFi-1
T10033 1240-1245 NNS denotes cells
T10034 1246-1250 VBD denotes were
T10035 1251-1259 VBN denotes cultured
T10036 1260-1262 IN denotes in
T10037 1263-1267 NNP denotes BEBM
T10038 1268-1278 JJ denotes serum-free
T10039 1279-1285 NN denotes medium
T10040 1286-1287 -LRB- denotes (
T10041 1287-1292 NNP denotes Lonza
T10042 1292-1293 -RRB- denotes )
T10043 1294-1298 IN denotes with
T10044 1299-1309 NN denotes supplement
T10045 1310-1316 NN denotes bullet
T10046 1317-1320 NN denotes kit
T10047 1321-1322 -LRB- denotes (
T10048 1322-1325 NNP denotes EGF
T10049 1325-1326 , denotes ,
T10050 1327-1341 NN denotes hydrocortisone
T10051 1341-1342 , denotes ,
T10052 1343-1349 JJ denotes bovine
T10053 1350-1359 JJ denotes pituitary
T10054 1360-1367 VB denotes extract
T10055 1367-1368 , denotes ,
T10056 1369-1380 NN denotes transferrin
T10057 1380-1381 , denotes ,
T10058 1382-1388 JJ denotes bovine
T10059 1389-1396 NN denotes insulin
T10060 1396-1397 , denotes ,
T10061 1398-1414 NN denotes triiodothyronine
T10062 1414-1415 , denotes ,
T10063 1416-1427 NN denotes epinephrine
T10064 1427-1428 , denotes ,
T10065 1429-1437 JJ denotes retinoic
T10066 1438-1442 NN denotes acid
T10067 1442-1443 -RRB- denotes )
T10068 1443-1444 , denotes ,
T10069 1445-1446 CD denotes 2
T10070 1447-1449 NNP denotes mM
T10071 1450-1461 NNP denotes L-glutamine
T10072 1461-1462 , denotes ,
T10073 1463-1466 CC denotes and
T10074 1467-1468 CD denotes 1
T10075 1469-1471 NNP denotes mM
T10076 1472-1478 NN denotes sodium
T10077 1479-1487 NN denotes pyruvate
T10078 1487-1488 . denotes .
T10079 1489-1494 NNS denotes PBMCs
T10080 1495-1499 IN denotes from
T10081 1500-1502 NNP denotes CF
T10082 1503-1511 NNS denotes patients
T10083 1512-1515 CC denotes and
T10084 1516-1524 NNS denotes controls
T10085 1525-1529 VBD denotes were
T10086 1530-1538 VBN denotes cultured
T10087 1539-1541 IN denotes in
T10088 1542-1551 NNP denotes RPMI-1640
T10089 1552-1553 -LRB- denotes (
T10090 1553-1560 NNP denotes Hyclone
T10091 1560-1561 -RRB- denotes )
T10092 1562-1574 VBN denotes supplemented
T10093 1575-1579 IN denotes with
T10094 1580-1582 CD denotes 10
T10095 1582-1583 NN denotes %
T10096 1584-1587 NNP denotes FBS
T10097 1587-1588 , denotes ,
T10098 1589-1590 CD denotes 2
T10099 1591-1593 NNP denotes mM
T10100 1594-1605 NNP denotes L-glutamine
T10101 1605-1606 , denotes ,
T10102 1607-1610 CC denotes and
T10103 1611-1612 CD denotes 1
T10104 1613-1615 NNP denotes mM
T10105 1616-1622 NN denotes sodium
T10106 1623-1631 NN denotes pyruvate
T10107 1632-1633 -LRB- denotes (
T10108 1633-1641 JJ denotes complete
T10109 1642-1646 NNP denotes RPMI
T10110 1646-1647 -RRB- denotes )
T10111 1647-1648 . denotes .
T10112 1649-1659 JJ denotes THP1-XBlue
T10113 1660-1665 NNS denotes cells
T10114 1666-1670 VBD denotes were
T10115 1671-1679 VBN denotes cultured
T10116 1680-1682 IN denotes in
T10117 1683-1691 JJ denotes complete
T10118 1692-1696 NNP denotes RPMI
T10119 1697-1701 IN denotes with
T10120 1702-1705 DT denotes the
T10121 1706-1714 NN denotes addition
T10122 1715-1717 IN denotes of
T10123 1718-1724 NN denotes zeocin
T10124 1725-1726 -LRB- denotes (
T10125 1726-1729 CD denotes 100
T10126 1730-1732 NN denotes µg
T10127 1732-1733 NN denotes /
T10128 1733-1735 NN denotes ml
T10129 1735-1736 -RRB- denotes )
T10130 1737-1739 TO denotes to
T10131 1740-1746 VB denotes select
T10132 1747-1750 IN denotes for
T10133 1751-1756 NNS denotes cells
T10134 1757-1767 VBG denotes expressing
T10135 1768-1771 DT denotes the
T10136 1772-1776 NNP denotes SEAP
T10137 1777-1782 NNP denotes NF-κB
T10138 1782-1783 NNP denotes /
T10139 1783-1787 NNP denotes AP-1
T10140 1788-1796 NN denotes reporter
T10141 1796-1797 . denotes .
T10142 1798-1803 RB denotes Prior
T10143 1804-1806 TO denotes to
T10144 1807-1818 NN denotes stimulation
T10145 1818-1819 , denotes ,
T10146 1820-1829 JJ denotes bronchial
T10147 1830-1840 JJ denotes epithelial
T10148 1841-1845 NN denotes cell
T10149 1846-1851 NNS denotes lines
T10150 1852-1856 VBD denotes were
T10151 1857-1863 VBN denotes plated
T10152 1864-1866 IN denotes in
T10153 1867-1873 JJ denotes coated
T10154 1874-1875 NNP denotes [
T10155 1875-1877 CD denotes 62
T10156 1877-1878 NNP denotes ]
T10157 1879-1886 JJ denotes 96-well
T10158 1887-1893 NNS denotes plates
T10159 1894-1895 -LRB- denotes (
T10160 1895-1897 NNP denotes BD
T10161 1898-1909 NNPS denotes Biosciences
T10162 1909-1910 -RRB- denotes )
T10163 1911-1913 IN denotes at
T10164 1914-1915 CD denotes 3
T10165 1915-1916 CD denotes ×
T10166 1916-1919 CD denotes 104
T10167 1920-1930 NN denotes cells/well
T10168 1931-1937 IN denotes unless
T10169 1938-1947 VBN denotes indicated
T10170 1947-1948 , denotes ,
T10171 1949-1952 CC denotes and
T10172 1953-1960 VBD denotes allowed
T10173 1961-1963 TO denotes to
T10174 1964-1970 VB denotes adhere
T10175 1971-1980 JJ denotes overnight
T10176 1980-1981 . denotes .
T10177 1982-1988 NNP denotes Plates
T10178 1989-1992 IN denotes for
T10179 1993-1995 NNP denotes S9
T10180 1996-1999 CC denotes and
T10181 2000-2005 NNP denotes IB3-1
T10182 2006-2018 NNS denotes stimulations
T10183 2019-2023 VBD denotes were
T10184 2024-2030 VBN denotes coated
T10185 2031-2033 IN denotes in
T10186 2034-2035 DT denotes a
T10187 2036-2043 NN denotes mixture
T10188 2044-2046 IN denotes of
T10189 2047-2053 JJ denotes bovine
T10190 2054-2059 NN denotes serum
T10191 2060-2067 NN denotes albumin
T10192 2068-2069 -LRB- denotes (
T10193 2069-2072 CD denotes 100
T10194 2073-2075 NN denotes µg
T10195 2075-2076 NN denotes /
T10196 2076-2078 NN denotes ml
T10197 2078-2079 -RRB- denotes )
T10198 2079-2080 , denotes ,
T10199 2081-2092 NN denotes fibronectin
T10200 2093-2094 -LRB- denotes (
T10201 2094-2096 CD denotes 10
T10202 2097-2099 NN denotes µg
T10203 2099-2100 NN denotes /
T10204 2100-2102 NN denotes ml
T10205 2102-2103 -RRB- denotes )
T10206 2103-2104 , denotes ,
T10207 2105-2108 CC denotes and
T10208 2109-2115 JJ denotes bovine
T10209 2116-2123 NN denotes collage
T10210 2124-2128 NN denotes type
T10211 2129-2130 PRP denotes I
T10212 2131-2132 -LRB- denotes (
T10213 2132-2134 CD denotes 30
T10214 2135-2137 NN denotes µg
T10215 2137-2138 NN denotes /
T10216 2138-2140 NN denotes ml
T10217 2140-2141 -RRB- denotes )
T10218 2142-2143 -LRB- denotes (
T10219 2143-2145 NNP denotes BD
T10220 2146-2157 NNPS denotes Biosciences
T10221 2157-2158 -RRB- denotes )
T10222 2158-2159 . denotes .
T10223 2160-2166 NNS denotes Plates
T10224 2167-2170 IN denotes for
T10225 2171-2177 NN denotes NuLi-1
T10226 2178-2181 CC denotes and
T10227 2182-2188 NN denotes CuFi-1
T10228 2189-2193 VBD denotes were
T10229 2194-2200 VBN denotes coated
T10230 2201-2205 IN denotes with
T10231 2206-2214 NN denotes collagen
T10232 2215-2219 NN denotes type
T10233 2220-2222 NNP denotes IV
T10234 2223-2224 -LRB- denotes (
T10235 2224-2226 CD denotes 60
T10236 2227-2229 NN denotes µg
T10237 2229-2230 NN denotes /
T10238 2230-2232 NN denotes ml
T10239 2232-2233 -RRB- denotes )
T10240 2234-2235 -LRB- denotes (
T10241 2235-2240 NNP denotes Sigma
T10242 2241-2248 NNP denotes Aldrich
T10243 2248-2249 -RRB- denotes )
T10244 2249-2250 . denotes .
T10245 2251-2256 NNS denotes PBMCs
T10246 2257-2261 VBD denotes were
T10247 2262-2268 VBN denotes plated
T10248 2269-2271 IN denotes in
T10249 2272-2279 JJ denotes 96-well
T10250 2280-2286 NNS denotes plates
T10251 2287-2289 IN denotes at
T10252 2290-2291 DT denotes a
T10253 2292-2299 NN denotes density
T10254 2300-2302 IN denotes of
T10255 2303-2306 CD denotes 1.5
T10256 2306-2307 CD denotes ×
T10257 2307-2310 CD denotes 105
T10258 2311-2321 NN denotes cells/well
T10259 2322-2324 IN denotes in
T10260 2325-2328 CD denotes 200
T10261 2329-2331 NN denotes µl
T10262 2332-2333 -LRB- denotes (
T10263 2333-2336 CD denotes 7.5
T10264 2336-2337 CD denotes ×
T10265 2337-2340 CD denotes 105
T10266 2341-2349 NNP denotes cells/mL
T10267 2349-2350 -RRB- denotes )
T10268 2350-2351 . denotes .
T10269 2352-2357 CD denotes THP-1
T10270 2358-2366 NN denotes reporter
T10271 2367-2372 NNS denotes cells
T10272 2373-2377 VBD denotes were
T10273 2378-2392 VBN denotes differentiated
T10274 2393-2397 IN denotes into
T10275 2398-2399 DT denotes a
T10276 2400-2415 JJ denotes macrophage-like
T10277 2416-2425 NN denotes phenotype
T10278 2426-2431 VBG denotes using
T10279 2432-2434 CD denotes 50
T10280 2435-2440 NN denotes ng/ml
T10281 2441-2443 IN denotes of
T10282 2444-2451 NN denotes phorbol
T10283 2452-2464 JJ denotes 12-myristate
T10284 2465-2475 JJ denotes 13-acetate
T10285 2476-2477 -LRB- denotes (
T10286 2477-2480 NNP denotes PMA
T10287 2480-2481 -RRB- denotes )
T10288 2482-2483 -LRB- denotes (
T10289 2483-2488 NNP denotes Sigma
T10290 2489-2496 NNP denotes Aldrich
T10291 2496-2497 -RRB- denotes )
T10292 2498-2501 IN denotes for
T10293 2502-2504 CD denotes 24
T10294 2505-2510 NNS denotes hours
T10295 2511-2513 IN denotes at
T10296 2514-2515 DT denotes a
T10297 2516-2523 NN denotes density
T10298 2524-2526 IN denotes of
T10299 2527-2528 CD denotes 1
T10300 2528-2529 CD denotes ×
T10301 2529-2532 CD denotes 105
T10302 2533-2543 NN denotes cells/well
T10303 2544-2546 IN denotes in
T10304 2547-2550 CD denotes 200
T10305 2551-2553 NN denotes µl
T10306 2554-2555 -LRB- denotes (
T10307 2555-2556 CD denotes 5
T10308 2556-2557 CD denotes ×
T10309 2557-2560 CD denotes 105
T10310 2561-2569 NN denotes cells/ml
T10311 2569-2570 -RRB- denotes )
T10312 2570-2571 . denotes .
T10313 2572-2577 NNS denotes Cells
T10314 2578-2582 VBD denotes were
T10315 2583-2589 VBN denotes washed
T10316 2590-2594 IN denotes with
T10317 2595-2598 NNP denotes PBS
T10318 2599-2602 CC denotes and
T10319 2603-2610 VBN denotes allowed
T10320 2611-2613 TO denotes to
T10321 2614-2618 VB denotes rest
T10322 2619-2620 DT denotes a
T10323 2621-2628 JJ denotes further
T10324 2629-2631 CD denotes 42
T10325 2632-2637 NNS denotes hours
T10326 2638-2643 RB denotes prior
T10327 2644-2646 TO denotes to
T10328 2647-2658 NN denotes stimulation
T10329 2658-2659 . denotes .
T10630 2661-2663 NNP denotes CF
T10631 2664-2667 CC denotes and
T10632 2668-2675 VB denotes control
T10633 2676-2683 JJ denotes subject
T10634 2684-2689 NNP denotes PBMCs
T10635 2690-2693 DT denotes The
T10636 2694-2703 NN denotes diagnosis
T10637 2704-2706 IN denotes of
T10638 2707-2709 NNP denotes CF
T10639 2710-2713 VBD denotes was
T10640 2714-2725 VBN denotes established
T10641 2726-2728 IN denotes by
T10642 2729-2736 JJ denotes typical
T10643 2737-2745 JJ denotes clinical
T10644 2746-2754 NNS denotes features
T10645 2754-2755 , denotes ,
T10646 2756-2765 VBD denotes increased
T10647 2766-2771 NN denotes sweat
T10648 2772-2780 NN denotes chloride
T10649 2781-2795 NNS denotes concentrations
T10650 2796-2797 -LRB- denotes (
T10651 2797-2798 CD denotes >
T10652 2798-2800 CD denotes 60
T10653 2801-2807 NN denotes mmol/l
T10654 2807-2808 -RRB- denotes )
T10655 2808-2809 , denotes ,
T10656 2810-2813 CC denotes and
T10657 2814-2823 NN denotes detection
T10658 2824-2826 IN denotes of
T10659 2827-2838 VBG denotes CF-inducing
T10660 2839-2848 NNS denotes mutations
T10661 2848-2849 . denotes .
T10662 2850-2853 DT denotes All
T10663 2854-2862 NNS denotes patients
T10664 2863-2867 IN denotes with
T10665 2868-2870 NNP denotes CF
T10666 2871-2875 VBD denotes were
T10667 2876-2886 RB denotes clinically
T10668 2887-2893 JJ denotes stable
T10669 2894-2896 IN denotes at
T10670 2897-2900 DT denotes the
T10671 2901-2905 NN denotes time
T10672 2906-2908 IN denotes of
T10673 2909-2914 NN denotes blood
T10674 2915-2923 NN denotes donation
T10675 2923-2924 , denotes ,
T10676 2925-2928 CC denotes and
T10677 2929-2931 PRP denotes we
T10678 2932-2940 VBD denotes excluded
T10679 2941-2944 DT denotes any
T10680 2945-2953 NNS denotes subjects
T10681 2954-2957 WP denotes who
T10682 2958-2962 VBD denotes were
T10683 2963-2972 VBG denotes receiving
T10684 2973-2981 JJ denotes systemic
T10685 2982-2997 NNS denotes corticosteroids
T10686 2998-3001 JJ denotes due
T10687 3002-3004 TO denotes to
T10688 3005-3014 JJ denotes potential
T10689 3015-3031 JJ denotes immunomodulatory
T10690 3032-3040 NN denotes activity
T10691 3040-3041 . denotes .
T10692 3042-3049 NNP denotes Control
T10693 3050-3057 NNS denotes samples
T10694 3058-3062 VBD denotes were
T10695 3063-3071 VBN denotes provided
T10696 3072-3074 IN denotes by
T10697 3075-3082 JJ denotes healthy
T10698 3083-3088 NN denotes adult
T10699 3089-3099 NNS denotes volunteers
T10700 3099-3100 . denotes .
T10701 3101-3103 IN denotes In
T10702 3104-3114 RB denotes previously
T10703 3115-3124 VBN denotes published
T10704 3125-3129 NN denotes work
T10705 3130-3132 PRP denotes we
T10706 3133-3137 VBP denotes have
T10707 3138-3150 VBN denotes demonstrated
T10708 3151-3155 IN denotes that
T10709 3156-3168 JJ denotes TLR-mediated
T10710 3169-3181 JJ denotes inflammatory
T10711 3182-3191 NNS denotes responses
T10712 3192-3195 VBP denotes are
T10713 3196-3202 JJ denotes stable
T10714 3203-3205 IN denotes in
T10715 3206-3212 NNS denotes humans
T10716 3213-3217 IN denotes from
T10717 3218-3223 NN denotes birth
T10718 3224-3226 TO denotes to
T10719 3227-3229 CD denotes 60
T10720 3230-3235 NNS denotes years
T10721 3236-3239 JJ denotes old
T10722 3240-3241 NNP denotes [
T10723 3241-3243 CD denotes 63
T10724 3243-3244 NNP denotes ]
T10725 3244-3245 , denotes ,
T10726 3246-3255 RB denotes therefore
T10727 3256-3259 VBD denotes did
T10728 3260-3263 RB denotes not
T10729 3264-3273 JJ denotes age-match
T10730 3274-3277 DT denotes the
T10731 3278-3280 NNP denotes CF
T10732 3281-3289 NNS denotes patients
T10733 3290-3293 CC denotes and
T10734 3294-3301 NN denotes control
T10735 3302-3310 NNS denotes subjects
T10736 3310-3311 . denotes .
T10737 3312-3322 JJ denotes Peripheral
T10738 3323-3328 NN denotes blood
T10739 3329-3332 VBD denotes was
T10740 3333-3342 VBN denotes collected
T10741 3343-3345 IN denotes in
T10742 3346-3352 NN denotes sodium
T10743 3353-3360 NN denotes heparin
T10744 3361-3366 NNS denotes tubes
T10745 3367-3368 -LRB- denotes (
T10746 3368-3370 NNP denotes BD
T10747 3371-3382 NNPS denotes Biosciences
T10748 3382-3383 -RRB- denotes )
T10749 3384-3387 CC denotes and
T10750 3388-3393 NNS denotes PBMCs
T10751 3394-3398 VBD denotes were
T10752 3399-3407 VBN denotes isolated
T10753 3408-3413 VBG denotes using
T10754 3414-3421 NN denotes density
T10755 3422-3430 NN denotes gradient
T10756 3431-3445 NN denotes centrifugation
T10757 3446-3448 IN denotes on
T10758 3449-3461 NNP denotes Ficoll-Paque
T10759 3461-3462 NNP denotes ™
T10760 3463-3467 NNP denotes Plus
T10761 3468-3469 -LRB- denotes (
T10762 3469-3471 NNP denotes GE
T10763 3472-3482 NNP denotes Healthcare
T10764 3482-3483 -RRB- denotes )
T10765 3483-3484 . denotes .
T10766 3485-3488 DT denotes The
T10767 3489-3494 NN denotes layer
T10768 3495-3505 VBG denotes containing
T10769 3506-3511 NNP denotes PBMCs
T10770 3512-3515 VBD denotes was
T10771 3516-3524 VBN denotes isolated
T10772 3524-3525 , denotes ,
T10773 3526-3532 VBN denotes washed
T10774 3533-3538 RB denotes twice
T10775 3539-3541 IN denotes in
T10776 3542-3545 NNP denotes PBS
T10777 3546-3549 CC denotes and
T10778 3550-3561 VBD denotes resuspended
T10779 3562-3564 IN denotes in
T10780 3565-3573 JJ denotes complete
T10781 3574-3578 NNP denotes RPMI
T10782 3578-3579 . denotes .
T10783 3580-3585 NNS denotes Cells
T10784 3586-3590 VBD denotes were
T10785 3591-3601 VBN denotes enumerated
T10786 3602-3604 IN denotes by
T10787 3605-3611 NN denotes trypan
T10788 3612-3616 JJ denotes blue
T10789 3617-3626 NN denotes exclusion
T10790 3627-3632 VBG denotes using
T10791 3633-3636 DT denotes the
T10792 3637-3645 NNP denotes Countess
T10793 3646-3655 VBN denotes automated
T10794 3656-3660 NN denotes cell
T10795 3661-3668 NN denotes counter
T10796 3669-3670 -LRB- denotes (
T10797 3670-3680 NNP denotes Invitrogen
T10798 3680-3681 -RRB- denotes )
T10799 3681-3682 . denotes .
T10800 3683-3686 IN denotes For
T10801 3687-3697 NN denotes derivation
T10802 3698-3700 IN denotes of
T10803 3701-3712 NNS denotes macrophages
T10804 3713-3717 IN denotes from
T10805 3718-3727 NNS denotes monocytes
T10806 3727-3728 , denotes ,
T10807 3729-3738 NNS denotes monocytes
T10808 3739-3743 VBD denotes were
T10809 3744-3751 VBN denotes allowed
T10810 3752-3754 TO denotes to
T10811 3755-3761 VB denotes adhere
T10812 3762-3764 TO denotes to
T10813 3765-3772 NN denotes plastic
T10814 3773-3776 IN denotes for
T10815 3777-3778 CD denotes 2
T10816 3779-3784 NNS denotes hours
T10817 3785-3787 IN denotes in
T10818 3788-3792 NNP denotes RPMI
T10819 3793-3797 CD denotes 1640
T10820 3798-3803 IN denotes after
T10821 3804-3809 WDT denotes which
T10822 3810-3822 JJ denotes non-adherent
T10823 3823-3828 NNS denotes cells
T10824 3829-3833 VBD denotes were
T10825 3834-3841 VBN denotes removed
T10826 3841-3842 . denotes .
T10827 3843-3852 NNS denotes Monocytes
T10828 3853-3857 VBD denotes were
T10829 3858-3865 VBN denotes allowed
T10830 3866-3868 TO denotes to
T10831 3869-3882 VB denotes differentiate
T10832 3883-3885 IN denotes in
T10833 3886-3890 NNP denotes RPMI
T10834 3891-3895 CD denotes 1640
T10835 3896-3908 VBN denotes supplemented
T10836 3909-3913 IN denotes with
T10837 3914-3916 CD denotes 10
T10838 3916-3917 NN denotes %
T10839 3918-3923 JJ denotes human
T10840 3924-3926 NNP denotes AB
T10841 3927-3932 NN denotes serum
T10842 3933-3936 IN denotes for
T10843 3937-3939 CD denotes 10
T10844 3940-3944 NNS denotes days
T10845 3944-3945 . denotes .
T11104 3947-3951 NNP denotes Cell
T11105 3952-3963 NN denotes stimulation
T11106 3964-3967 CC denotes and
T11107 3968-3976 NN denotes cytokine
T11108 3977-3991 NN denotes quantification
T11109 3992-4001 NNP denotes Bronchial
T11110 4002-4012 JJ denotes epithelial
T11111 4013-4018 NNS denotes cells
T11112 4019-4023 VBD denotes were
T11113 4024-4030 VBN denotes plated
T11114 4031-4034 CC denotes and
T11115 4035-4042 VBN denotes allowed
T11116 4043-4045 TO denotes to
T11117 4046-4052 VB denotes adhere
T11118 4053-4062 JJ denotes overnight
T11119 4063-4068 RB denotes prior
T11120 4069-4071 TO denotes to
T11121 4072-4083 NN denotes stimulation
T11122 4083-4084 . denotes .
T11123 4085-4094 NNP denotes Bronchial
T11124 4095-4105 JJ denotes epithelial
T11125 4106-4111 NNS denotes cells
T11126 4112-4116 VBD denotes were
T11127 4117-4123 VBN denotes rested
T11128 4124-4126 CC denotes or
T11129 4127-4133 VBN denotes primed
T11130 4134-4138 IN denotes with
T11131 4139-4142 NNP denotes LPS
T11132 4143-4146 IN denotes for
T11133 4147-4148 CD denotes 5
T11134 4149-4154 NNS denotes hours
T11135 4155-4158 CC denotes and
T11136 4159-4169 VBN denotes stimulated
T11137 4170-4174 IN denotes with
T11138 4175-4179 JJ denotes live
T11139 4180-4182 NNP denotes P.
T11140 4183-4193 NN denotes aeruginosa
T11141 4194-4198 NNP denotes PAO1
T11142 4199-4201 CC denotes or
T11143 4202-4205 NNP denotes ATP
T11144 4206-4209 IN denotes for
T11145 4210-4213 DT denotes the
T11146 4214-4219 NNS denotes times
T11147 4220-4229 VBD denotes indicated
T11148 4229-4230 . denotes .
T11149 4231-4236 NNS denotes PBMCs
T11150 4237-4240 CC denotes and
T11151 4241-4246 CD denotes THP-1
T11152 4247-4255 NN denotes reporter
T11153 4256-4261 NNS denotes cells
T11154 4262-4266 VBD denotes were
T11155 4267-4273 RB denotes either
T11156 4274-4280 VBN denotes rested
T11157 4281-4283 CC denotes or
T11158 4284-4290 VBN denotes primed
T11159 4291-4295 IN denotes with
T11160 4296-4299 NNP denotes LPS
T11161 4300-4301 -LRB- denotes (
T11162 4301-4310 NNP denotes Invivogen
T11163 4310-4311 -RRB- denotes )
T11164 4312-4314 CC denotes or
T11165 4315-4326 JJ denotes heat-killed
T11166 4327-4331 NNP denotes PAO1
T11167 4332-4341 JJ denotes overnight
T11168 4342-4343 -LRB- denotes (
T11169 4343-4345 CD denotes 16
T11170 4346-4351 NNS denotes hours
T11171 4351-4352 -RRB- denotes )
T11172 4352-4353 . denotes .
T11173 4354-4357 DT denotes The
T11174 4358-4362 JJ denotes next
T11175 4363-4366 NN denotes day
T11176 4367-4370 DT denotes the
T11177 4371-4376 NNS denotes cells
T11178 4377-4381 VBD denotes were
T11179 4382-4392 VBN denotes challenged
T11180 4393-4397 IN denotes with
T11181 4398-4402 JJ denotes live
T11182 4403-4407 NNP denotes PAO1
T11183 4407-4408 , denotes ,
T11184 4409-4418 NNP denotes PAO1ΔexsA
T11185 4418-4419 , denotes ,
T11186 4420-4423 NNP denotes ATP
T11187 4424-4425 -LRB- denotes (
T11188 4425-4434 NNP denotes Invivogen
T11189 4434-4435 -RRB- denotes )
T11190 4435-4436 , denotes ,
T11191 4437-4439 CC denotes or
T11192 4440-4444 NNP denotes Poly
T11193 4444-4445 -LRB- denotes (
T11194 4445-4447 NNP denotes dA
T11195 4447-4448 : denotes :
T11196 4448-4450 NN denotes dT
T11197 4450-4451 -RRB- denotes )
T11198 4452-4453 -LRB- denotes (
T11199 4453-4458 NNP denotes Sigma
T11200 4459-4466 NNP denotes Aldrich
T11201 4466-4467 -RRB- denotes )
T11202 4468-4471 IN denotes for
T11203 4472-4475 DT denotes the
T11204 4476-4481 NNS denotes times
T11205 4482-4491 VBD denotes indicated
T11206 4492-4493 -LRB- denotes (
T11207 4493-4496 VB denotes see
T11208 4497-4501 NNP denotes Fig.
T11209 4502-4503 CD denotes 1
T11210 4503-4504 -RRB- denotes )
T11211 4504-4505 . denotes .
T11212 4506-4509 IN denotes For
T11213 4510-4522 NNS denotes stimulations
T11214 4523-4527 IN denotes with
T11215 4528-4532 NNP denotes Poly
T11216 4532-4533 -LRB- denotes (
T11217 4533-4535 NNP denotes dA
T11218 4535-4536 : denotes :
T11219 4536-4538 NN denotes dT
T11220 4538-4539 -RRB- denotes )
T11221 4539-4540 , denotes ,
T11222 4541-4554 NN denotes lipofectamine
T11223 4555-4558 NNP denotes LTX
T11224 4559-4562 VBD denotes was
T11225 4563-4567 VBN denotes used
T11226 4568-4570 IN denotes at
T11227 4571-4572 DT denotes a
T11228 4573-4574 CD denotes 1
T11229 4574-4575 NN denotes ∶
T11230 4575-4576 CD denotes 1
T11231 4577-4578 -LRB- denotes (
T11232 4578-4579 FW denotes w
T11233 4579-4580 FW denotes ∶
T11234 4580-4581 FW denotes v
T11235 4581-4582 -RRB- denotes )
T11236 4583-4588 NN denotes ratio
T11237 4589-4591 IN denotes of
T11238 4592-4594 NN denotes µg
T11239 4595-4597 IN denotes of
T11240 4598-4601 NNP denotes DNA
T11241 4602-4604 TO denotes to
T11242 4605-4607 VB denotes µl
T11243 4608-4610 IN denotes of
T11244 4611-4624 NN denotes lipofectamine
T11245 4625-4628 CC denotes and
T11246 4629-4632 VBD denotes was
T11247 4633-4638 JJ denotes mixed
T11248 4639-4641 CD denotes 30
T11249 4642-4649 NNS denotes minutes
T11250 4650-4655 RB denotes prior
T11251 4656-4658 TO denotes to
T11252 4659-4670 NN denotes stimulation
T11253 4670-4671 . denotes .
T11254 4672-4675 DT denotes The
T11255 4676-4681 JJ denotes NF-κB
T11256 4682-4691 NN denotes inhibitor
T11257 4692-4702 NN denotes Bay11-7082
T11258 4703-4704 -LRB- denotes (
T11259 4704-4713 NNP denotes Invivogen
T11260 4713-4714 -RRB- denotes )
T11261 4715-4718 VBD denotes was
T11262 4719-4724 VBN denotes added
T11263 4725-4727 TO denotes to
T11264 4728-4736 NNS denotes cultures
T11265 4737-4738 CD denotes 1
T11266 4739-4743 NN denotes hour
T11267 4744-4749 RB denotes prior
T11268 4750-4752 TO denotes to
T11269 4753-4760 VBG denotes priming
T11270 4760-4761 . denotes .
T11271 4762-4764 IN denotes If
T11272 4765-4767 DT denotes no
T11273 4768-4775 NN denotes priming
T11274 4776-4779 VBD denotes was
T11275 4780-4788 VBN denotes involved
T11276 4788-4789 , denotes ,
T11277 4790-4799 NN denotes inhibitor
T11278 4800-4803 VBD denotes was
T11279 4804-4809 VBN denotes added
T11280 4810-4811 CD denotes 1
T11281 4812-4816 NN denotes hour
T11282 4817-4822 RB denotes prior
T11283 4823-4825 TO denotes to
T11284 4826-4838 JJ denotes inflammasome
T11285 4839-4850 NN denotes stimulation
T11286 4850-4851 . denotes .
T11287 4852-4855 DT denotes The
T11288 4856-4860 NNP denotes CFTR
T11289 4861-4870 NN denotes inhibitor
T11290 4871-4881 NNP denotes CFTRinh172
T11291 4882-4883 -LRB- denotes (
T11292 4883-4888 NNP denotes Sigma
T11293 4889-4896 NNP denotes Aldrich
T11294 4896-4897 -RRB- denotes )
T11295 4898-4901 VBD denotes was
T11296 4902-4907 VBN denotes added
T11297 4908-4910 TO denotes to
T11298 4911-4919 NNS denotes cultures
T11299 4920-4922 CD denotes 18
T11300 4923-4928 NNS denotes hours
T11301 4929-4934 RB denotes prior
T11302 4935-4937 TO denotes to
T11303 4938-4950 JJ denotes inflammasome
T11304 4951-4962 NN denotes stimulation
T11305 4962-4963 . denotes .
T11306 4964-4967 DT denotes The
T11307 4968-4977 JJ denotes caspase-1
T11308 4978-4987 NN denotes inhibitor
T11309 4988-4998 NNP denotes z-YVAD-fmk
T11310 4999-5000 -LRB- denotes (
T11311 5000-5009 NNP denotes Biovision
T11312 5009-5010 -RRB- denotes )
T11313 5011-5014 VBD denotes was
T11314 5015-5020 VBN denotes added
T11315 5021-5023 TO denotes to
T11316 5024-5032 NNS denotes cultures
T11317 5033-5034 CD denotes 1
T11318 5035-5039 NN denotes hour
T11319 5040-5045 RB denotes prior
T11320 5046-5048 TO denotes to
T11321 5049-5061 JJ denotes inflammasome
T11322 5062-5073 NN denotes stimulation
T11323 5073-5074 . denotes .
T11324 5075-5087 NNS denotes Supernatants
T11325 5088-5092 VBD denotes were
T11326 5093-5102 VBN denotes collected
T11327 5103-5106 CC denotes and
T11328 5107-5113 VBN denotes stored
T11329 5114-5116 IN denotes at
T11330 5117-5118 CD denotes −
T11331 5118-5120 CD denotes 20
T11332 5120-5121 NN denotes °
T11333 5121-5123 NNP denotes C.
T11334 5124-5133 NNP denotes Cytokines
T11335 5134-5142 VBD denotes released
T11336 5143-5147 IN denotes into
T11337 5148-5160 NNS denotes supernatants
T11338 5161-5165 IN denotes from
T11339 5166-5171 NNP denotes PBMCs
T11340 5172-5182 VBD denotes stimulated
T11341 5183-5187 IN denotes with
T11342 5188-5200 JJ denotes inflammasome
T11343 5201-5211 NNS denotes activators
T11344 5212-5216 VBD denotes were
T11345 5217-5227 VBN denotes quantified
T11346 5228-5233 VBG denotes using
T11347 5234-5242 NN denotes sandwich
T11348 5243-5248 NNP denotes ELISA
T11349 5249-5250 -LRB- denotes (
T11350 5250-5261 NNP denotes eBioscience
T11351 5261-5262 -RRB- denotes )
T11352 5262-5263 . denotes .
T11553 5265-5279 VBG denotes Immunoblotting
T11554 5280-5281 CD denotes 1
T11555 5281-5282 CD denotes ×
T11556 5282-5285 CD denotes 106
T11557 5286-5291 NNS denotes cells
T11558 5292-5296 VBD denotes were
T11559 5297-5303 VBN denotes seeded
T11560 5304-5306 IN denotes in
T11561 5307-5314 JJ denotes 12-well
T11562 5315-5321 NNS denotes plates
T11563 5321-5322 , denotes ,
T11564 5323-5333 VBN denotes stimulated
T11565 5334-5336 IN denotes as
T11566 5337-5346 VBN denotes indicated
T11567 5346-5347 , denotes ,
T11568 5348-5351 CC denotes and
T11569 5352-5357 VBD denotes lysed
T11570 5358-5360 IN denotes in
T11571 5361-5365 NNP denotes RIPA
T11572 5366-5372 NN denotes buffer
T11573 5373-5385 VBN denotes supplemented
T11574 5386-5390 IN denotes with
T11575 5391-5395 NNP denotes Halt
T11576 5396-5404 NN denotes protease
T11577 5405-5408 CC denotes and
T11578 5409-5420 NN denotes phosphatase
T11579 5421-5430 NN denotes inhibitor
T11580 5431-5439 NN denotes cocktail
T11581 5440-5441 -LRB- denotes (
T11582 5441-5447 NNP denotes Thermo
T11583 5448-5458 NNP denotes Scientific
T11584 5458-5459 -RRB- denotes )
T11585 5459-5460 . denotes .
T11586 5461-5468 NN denotes Protein
T11587 5469-5483 NNS denotes concentrations
T11588 5484-5488 VBD denotes were
T11589 5489-5499 VBN denotes determined
T11590 5500-5502 IN denotes by
T11591 5503-5511 NNP denotes Bradford
T11592 5512-5517 NN denotes assay
T11593 5518-5519 -LRB- denotes (
T11594 5519-5525 NNP denotes Thermo
T11595 5526-5536 NNP denotes Scientific
T11596 5536-5537 -RRB- denotes )
T11597 5537-5538 . denotes .
T11598 5539-5546 NNS denotes Lysates
T11599 5547-5551 VBD denotes were
T11600 5552-5560 VBN denotes resolved
T11601 5561-5563 IN denotes by
T11602 5564-5579 NNS denotes electrophoreses
T11603 5580-5582 IN denotes on
T11604 5583-5585 CD denotes 10
T11605 5585-5586 NN denotes %
T11606 5587-5605 JJ denotes SDS-polyacrylamide
T11607 5606-5610 NNS denotes gels
T11608 5611-5614 CC denotes and
T11609 5615-5626 VBN denotes transferred
T11610 5627-5631 IN denotes onto
T11611 5632-5636 NNP denotes PVDF
T11612 5637-5646 NNS denotes membranes
T11613 5647-5648 -LRB- denotes (
T11614 5648-5657 NNP denotes Millipore
T11615 5657-5658 -RRB- denotes )
T11616 5658-5659 . denotes .
T11617 5660-5665 NNS denotes Blots
T11618 5666-5670 VBD denotes were
T11619 5671-5678 VBN denotes blocked
T11620 5679-5682 IN denotes for
T11621 5683-5684 CD denotes 1
T11622 5685-5689 NN denotes hour
T11623 5690-5692 IN denotes at
T11624 5693-5697 NN denotes room
T11625 5698-5709 NN denotes temperature
T11626 5710-5713 CC denotes and
T11627 5714-5720 VBD denotes probed
T11628 5721-5730 JJ denotes overnight
T11629 5731-5733 IN denotes at
T11630 5734-5735 CD denotes 4
T11631 5735-5736 CD denotes °
T11632 5736-5737 NNP denotes C
T11633 5738-5741 IN denotes for
T11634 5742-5751 NNP denotes pro-IL-1β
T11635 5752-5753 -LRB- denotes (
T11636 5753-5758 NNP denotes Santa
T11637 5759-5763 NNP denotes Cruz
T11638 5763-5764 -RRB- denotes )
T11639 5764-5765 , denotes ,
T11640 5766-5770 NNP denotes IκBα
T11641 5771-5772 -LRB- denotes (
T11642 5772-5776 NNP denotes Cell
T11643 5777-5786 NNP denotes Signaling
T11644 5786-5787 -RRB- denotes )
T11645 5787-5788 , denotes ,
T11646 5789-5791 CC denotes or
T11647 5792-5799 NN denotes β-actin
T11648 5800-5801 -LRB- denotes (
T11649 5801-5805 NNP denotes Cell
T11650 5806-5815 NNP denotes Signaling
T11651 5815-5816 -RRB- denotes )
T11652 5816-5817 . denotes .
T11653 5818-5823 NNS denotes Blots
T11654 5824-5828 VBD denotes were
T11655 5829-5841 RB denotes subsequently
T11656 5842-5848 VBN denotes probed
T11657 5849-5853 IN denotes with
T11658 5854-5875 JJ denotes fluorescently-labeled
T11659 5876-5885 JJ denotes secondary
T11660 5886-5896 NNS denotes antibodies
T11661 5896-5897 , denotes ,
T11662 5898-5903 NNP denotes IRDye
T11663 5903-5904 NNP denotes ®
T11664 5905-5908 CD denotes 680
T11665 5909-5911 CC denotes or
T11666 5912-5917 CD denotes 800CW
T11667 5918-5919 -LRB- denotes (
T11668 5919-5925 NNP denotes LI-COR
T11669 5926-5937 NNPS denotes Biosciences
T11670 5937-5938 -RRB- denotes )
T11671 5939-5942 IN denotes for
T11672 5943-5944 CD denotes 1
T11673 5945-5949 NN denotes hour
T11674 5949-5950 . denotes .
T11675 5951-5955 DT denotes Both
T11676 5956-5964 VBG denotes blocking
T11677 5965-5968 CC denotes and
T11678 5969-5976 VBG denotes probing
T11679 5977-5982 NNS denotes steps
T11680 5983-5987 VBD denotes were
T11681 5988-5995 VBN denotes carried
T11682 5996-5999 RP denotes out
T11683 6000-6002 IN denotes in
T11684 6003-6016 JJ denotes tris-buffered
T11685 6017-6023 NN denotes saline
T11686 6024-6025 -LRB- denotes (
T11687 6025-6026 NNP denotes G
T11688 6027-6038 NNPS denotes Biosciences
T11689 6038-6039 -RRB- denotes )
T11690 6040-6050 VBG denotes containing
T11691 6051-6052 CD denotes 5
T11692 6052-6053 NN denotes %
T11693 6054-6060 JJ denotes bovine
T11694 6061-6066 NN denotes serum
T11695 6067-6074 NN denotes albumin
T11696 6075-6078 CC denotes and
T11697 6079-6082 CD denotes 0.1
T11698 6082-6083 NN denotes %
T11699 6084-6089 NNP denotes TWEEN
T11700 6090-6092 CD denotes 20
T11701 6093-6094 -LRB- denotes (
T11702 6094-6104 NN denotes Calbiochem
T11703 6104-6105 -RRB- denotes )
T11704 6105-6106 . denotes .
T11705 6107-6112 NNS denotes Blots
T11706 6113-6117 VBD denotes were
T11707 6118-6124 VBN denotes imaged
T11708 6125-6127 IN denotes on
T11709 6128-6129 DT denotes a
T11710 6130-6136 NNP denotes LI-COR
T11711 6137-6144 NNP denotes Odyssey
T11712 6145-6153 VBD denotes infrared
T11713 6154-6161 NN denotes imaging
T11714 6162-6168 NN denotes system
T11715 6169-6170 -LRB- denotes (
T11716 6170-6176 NNP denotes LI-COR
T11717 6177-6188 NNPS denotes Biosciences
T11718 6188-6189 -RRB- denotes )
T11719 6190-6193 CC denotes and
T11720 6194-6204 VBD denotes quantified
T11721 6205-6210 VBG denotes using
T11722 6211-6214 DT denotes the
T11723 6215-6223 VBN denotes included
T11724 6224-6232 NN denotes analysis
T11725 6233-6241 NN denotes software
T11726 6241-6242 . denotes .
T11929 6244-6258 NN denotes Quantification
T11930 6259-6261 IN denotes of
T11931 6262-6271 JJ denotes caspase-1
T11932 6272-6280 NN denotes activity
T11933 6281-6290 NNP denotes Bronchial
T11934 6291-6301 JJ denotes epithelial
T11935 6302-6307 NNS denotes cells
T11936 6308-6312 VBD denotes were
T11937 6313-6319 VBN denotes plated
T11938 6320-6322 IN denotes in
T11939 6323-6329 JJ denotes 6-well
T11940 6330-6336 NNS denotes plates
T11941 6337-6339 IN denotes at
T11942 6340-6341 CD denotes 5
T11943 6341-6342 CD denotes ×
T11944 6342-6345 CD denotes 105
T11945 6346-6356 NN denotes cells/well
T11946 6357-6366 JJ denotes overnight
T11947 6366-6367 . denotes .
T11948 6368-6373 NNS denotes Cells
T11949 6374-6378 VBD denotes were
T11950 6379-6385 VBN denotes primed
T11951 6386-6390 IN denotes with
T11952 6391-6394 NNP denotes LPS
T11953 6395-6398 IN denotes for
T11954 6399-6400 CD denotes 5
T11955 6401-6406 NNS denotes hours
T11956 6407-6410 CC denotes and
T11957 6411-6421 VBN denotes stimulated
T11958 6422-6426 IN denotes with
T11959 6427-6430 NNP denotes ATP
T11960 6431-6434 IN denotes for
T11961 6435-6436 CD denotes 1
T11962 6437-6441 NN denotes hour
T11963 6442-6444 CC denotes or
T11964 6445-6455 VBN denotes stimulated
T11965 6456-6460 IN denotes with
T11966 6461-6465 JJ denotes live
T11967 6466-6470 NN denotes PAO1
T11968 6471-6474 IN denotes for
T11969 6475-6476 CD denotes 3
T11970 6477-6482 NNS denotes hours
T11971 6482-6483 . denotes .
T11972 6484-6489 NNS denotes PBMCs
T11973 6490-6494 VBD denotes were
T11974 6495-6505 VBN denotes stimulated
T11975 6506-6509 DT denotes the
T11976 6510-6514 JJ denotes same
T11977 6515-6518 NN denotes day
T11978 6519-6521 IN denotes as
T11979 6522-6527 NN denotes blood
T11980 6528-6536 NN denotes donation
T11981 6536-6537 . denotes .
T11982 6538-6543 NNS denotes PBMCs
T11983 6544-6548 VBD denotes were
T11984 6549-6555 VBN denotes seeded
T11985 6556-6558 IN denotes in
T11986 6559-6560 DT denotes a
T11987 6561-6568 JJ denotes 96-well
T11988 6569-6574 NN denotes plate
T11989 6575-6577 IN denotes at
T11990 6578-6579 DT denotes a
T11991 6580-6587 NN denotes density
T11992 6588-6590 IN denotes of
T11993 6591-6594 CD denotes 4.5
T11994 6594-6595 CD denotes ×
T11995 6595-6598 CD denotes 105
T11996 6599-6609 NN denotes cells/well
T11997 6610-6611 -LRB- denotes (
T11998 6611-6614 CD denotes 2.5
T11999 6614-6615 CD denotes ×
T12000 6615-6618 CD denotes 106
T12001 6619-6627 NN denotes cells/ml
T12002 6627-6628 -RRB- denotes )
T12003 6628-6629 , denotes ,
T12004 6630-6636 VBN denotes primed
T12005 6637-6641 IN denotes with
T12006 6642-6645 NNP denotes LPS
T12007 6646-6649 IN denotes for
T12008 6650-6651 CD denotes 5
T12009 6652-6657 NNS denotes hours
T12010 6658-6661 CC denotes and
T12011 6662-6666 RB denotes then
T12012 6667-6677 VBN denotes stimulated
T12013 6678-6682 IN denotes with
T12014 6683-6686 NNP denotes ATP
T12015 6687-6690 IN denotes for
T12016 6691-6692 CD denotes 1
T12017 6693-6697 NN denotes hour
T12018 6698-6700 CC denotes or
T12019 6701-6705 NNP denotes Poly
T12020 6705-6706 -LRB- denotes (
T12021 6706-6708 NNP denotes dA
T12022 6708-6709 : denotes :
T12023 6709-6711 NN denotes dT
T12024 6711-6712 -RRB- denotes )
T12025 6713-6716 IN denotes for
T12026 6717-6718 CD denotes 3
T12027 6719-6724 NNS denotes hours
T12028 6725-6727 CC denotes or
T12029 6728-6738 VBN denotes stimulated
T12030 6739-6743 IN denotes with
T12031 6744-6748 JJ denotes live
T12032 6749-6753 NN denotes PAO1
T12033 6754-6757 IN denotes for
T12034 6758-6759 CD denotes 3
T12035 6760-6765 NNS denotes hours
T12036 6765-6766 . denotes .
T12037 6767-6776 NN denotes Caspase-1
T12038 6777-6785 NN denotes activity
T12039 6786-6789 VBD denotes was
T12040 6790-6798 VBN denotes measured
T12041 6799-6804 VBG denotes using
T12042 6805-6810 NNP denotes FLICA
T12043 6811-6812 -LRB- denotes (
T12044 6812-6827 NNP denotes Immunochemistry
T12045 6828-6840 NNPS denotes Technologies
T12046 6840-6841 -RRB- denotes )
T12047 6841-6842 , denotes ,
T12048 6843-6844 DT denotes a
T12049 6845-6859 JJ denotes cell-permeable
T12050 6860-6871 JJ denotes fluorescent
T12051 6872-6877 NN denotes probe
T12052 6878-6879 -LRB- denotes (
T12053 6879-6891 JJ denotes FAM-YVAD-fmk
T12054 6891-6892 -RRB- denotes )
T12055 6893-6897 WDT denotes that
T12056 6898-6903 VBZ denotes binds
T12057 6904-6910 JJ denotes active
T12058 6911-6920 NN denotes caspase-1
T12059 6920-6921 . denotes .
T12060 6922-6927 NNS denotes Cells
T12061 6928-6932 VBD denotes were
T12062 6933-6942 VBN denotes incubated
T12063 6943-6944 CD denotes 1
T12064 6945-6949 NN denotes hour
T12065 6950-6954 IN denotes with
T12066 6955-6960 NNP denotes FLICA
T12067 6961-6963 IN denotes at
T12068 6964-6966 CD denotes 37
T12069 6966-6967 CD denotes °
T12070 6967-6968 NNP denotes C
T12071 6969-6972 CC denotes and
T12072 6973-6980 VBN denotes stained
T12073 6981-6985 IN denotes with
T12074 6986-7003 JJ denotes PE-Cy7-conjugated
T12075 7004-7013 JJ denotes anti-CD14
T12076 7014-7024 NNS denotes antibodies
T12077 7025-7026 -LRB- denotes (
T12078 7026-7037 NNP denotes eBioscience
T12079 7037-7038 -RRB- denotes )
T12080 7039-7041 TO denotes to
T12081 7042-7050 VB denotes identify
T12082 7051-7060 NNS denotes monocytes
T12083 7060-7061 . denotes .
T12084 7062-7065 DT denotes The
T12085 7066-7072 VBG denotes gating
T12086 7073-7081 NN denotes strategy
T12087 7082-7091 VBD denotes consisted
T12088 7092-7094 IN denotes of
T12089 7095-7104 VBG denotes including
T12090 7105-7109 JJ denotes live
T12091 7110-7115 NNS denotes cells
T12092 7116-7120 WDT denotes that
T12093 7121-7125 VBD denotes were
T12094 7126-7130 CD denotes CD14
T12095 7131-7139 JJ denotes positive
T12096 7140-7145 WDT denotes which
T12097 7146-7150 VBD denotes were
T12098 7151-7163 RB denotes subsequently
T12099 7164-7172 VBN denotes analyzed
T12100 7173-7176 IN denotes for
T12101 7177-7180 DT denotes the
T12102 7181-7190 NN denotes frequency
T12103 7191-7193 IN denotes of
T12104 7194-7199 NNP denotes FLICA
T12105 7200-7208 JJ denotes positive
T12106 7209-7214 NNS denotes cells
T12107 7214-7215 . denotes .
T12209 7217-7222 NNP denotes NF-κB
T12210 7222-7223 NNP denotes /
T12211 7223-7227 NNP denotes AP-1
T12212 7228-7236 NNP denotes Activity
T12213 7237-7242 NNP denotes Assay
T12214 7243-7255 NNPS denotes Supernatants
T12215 7256-7260 IN denotes from
T12216 7261-7266 CD denotes THP-1
T12217 7267-7275 NN denotes reporter
T12218 7276-7281 NNS denotes cells
T12219 7282-7286 VBD denotes were
T12220 7287-7296 VBN denotes incubated
T12221 7297-7301 IN denotes with
T12222 7302-7313 JJ denotes Quanti-Blue
T12223 7314-7323 NN denotes substrate
T12224 7324-7325 -LRB- denotes (
T12225 7325-7334 NNP denotes Invivogen
T12226 7334-7335 -RRB- denotes )
T12227 7336-7338 IN denotes at
T12228 7339-7341 CD denotes 37
T12229 7341-7342 CD denotes °
T12230 7342-7343 NNP denotes C
T12231 7344-7347 CC denotes and
T12232 7348-7355 VBN denotes allowed
T12233 7356-7358 TO denotes to
T12234 7359-7366 VB denotes develop
T12235 7367-7370 IN denotes for
T12236 7371-7373 CD denotes 16
T12237 7374-7376 CD denotes 18
T12238 7377-7382 NNS denotes hours
T12239 7382-7383 . denotes .
T12240 7384-7395 JJ denotes Quanti-Blue
T12241 7396-7404 VBZ denotes contains
T12242 7405-7406 DT denotes a
T12243 7407-7416 NN denotes substrate
T12244 7417-7420 IN denotes for
T12245 7421-7429 JJ denotes alkaline
T12246 7430-7441 NN denotes phosphatase
T12247 7442-7445 CC denotes and
T12248 7446-7453 NNS denotes changes
T12249 7454-7456 IN denotes in
T12250 7457-7460 DT denotes the
T12251 7461-7467 NN denotes amount
T12252 7468-7470 IN denotes of
T12253 7471-7476 NNP denotes NF-κB
T12254 7476-7477 NNP denotes /
T12255 7477-7481 NNP denotes AP-1
T12256 7482-7490 NN denotes activity
T12257 7491-7495 VBD denotes were
T12258 7496-7506 VBN denotes quantified
T12259 7507-7509 IN denotes by
T12260 7510-7517 JJ denotes optical
T12261 7518-7525 NN denotes density
T12262 7526-7527 -LRB- denotes (
T12263 7527-7528 FW denotes λ
T12264 7529-7530 SYM denotes =
T12265 7531-7534 CD denotes 655
T12266 7534-7535 -RRB- denotes )
T12267 7536-7544 VBN denotes measured
T12268 7545-7550 VBG denotes using
T12269 7551-7552 DT denotes a
T12270 7553-7563 NNP denotes SpectraMax
T12271 7564-7567 CD denotes 384
T12272 7568-7572 NNP denotes Plus
T12273 7573-7578 NN denotes plate
T12274 7579-7585 NN denotes reader
T12275 7586-7589 CC denotes and
T12276 7590-7597 NNP denotes SoftMax
T12277 7598-7601 FW denotes Pro
T12278 7602-7610 NN denotes software
T12279 7611-7612 -LRB- denotes (
T12280 7612-7621 JJ denotes Molecular
T12281 7622-7629 NNPS denotes Devices
T12282 7629-7630 -RRB- denotes )
T12283 7630-7631 . denotes .
T12443 7633-7642 JJ denotes Bacterial
T12444 7643-7650 NNS denotes strains
T12445 7651-7653 NNP denotes P.
T12446 7654-7664 NN denotes aeruginosa
T12447 7665-7675 NN denotes laboratory
T12448 7676-7683 NNS denotes strains
T12449 7684-7688 NNP denotes PAO1
T12450 7689-7692 CC denotes and
T12451 7693-7696 DT denotes the
T12452 7697-7706 NNP denotes PAO1ΔexsA
T12453 7707-7713 JJ denotes mutant
T12454 7714-7718 VBD denotes were
T12455 7719-7727 VBN denotes obtained
T12456 7728-7732 IN denotes from
T12457 7733-7736 NNP denotes Dr.
T12458 7737-7743 NNP denotes Robert
T12459 7744-7751 NNP denotes Hancock
T12460 7751-7752 . denotes .
T12461 7753-7755 NNP denotes P.
T12462 7756-7766 NN denotes aeruginosa
T12463 7767-7774 NNS denotes strains
T12464 7775-7779 NNP denotes PAO1
T12465 7780-7783 CC denotes and
T12466 7784-7793 NNP denotes PAO1ΔexsA
T12467 7794-7798 VBD denotes were
T12468 7799-7804 VBN denotes grown
T12469 7805-7809 IN denotes from
T12470 7810-7819 JJ denotes overnight
T12471 7820-7828 NNS denotes cultures
T12472 7829-7831 IN denotes in
T12473 7832-7837 NNP denotes Luria
T12474 7838-7845 NNP denotes Bertani
T12475 7846-7847 -LRB- denotes (
T12476 7847-7849 NNP denotes LB
T12477 7849-7850 -RRB- denotes )
T12478 7851-7856 NN denotes broth
T12479 7857-7860 CC denotes and
T12480 7861-7863 NNP denotes LB
T12481 7863-7864 NNP denotes +
T12482 7864-7876 NNP denotes streptomycin
T12483 7877-7878 -LRB- denotes (
T12484 7878-7881 CD denotes 150
T12485 7882-7884 NN denotes µg
T12486 7884-7885 NN denotes /
T12487 7885-7887 NN denotes ml
T12488 7887-7888 -RRB- denotes )
T12489 7889-7894 IN denotes until
T12490 7895-7910 JJ denotes mid-logarithmic
T12491 7911-7916 NN denotes phase
T12492 7916-7917 . denotes .
T12493 7918-7923 NNS denotes Cells
T12494 7924-7928 VBD denotes were
T12495 7929-7935 VBN denotes washed
T12496 7936-7940 RB denotes once
T12497 7941-7943 IN denotes in
T12498 7944-7947 NNP denotes PBS
T12499 7948-7951 CC denotes and
T12500 7952-7963 VBD denotes resuspended
T12501 7964-7966 IN denotes in
T12502 7967-7970 NNP denotes PBS
T12503 7971-7973 TO denotes to
T12504 7974-7976 DT denotes an
T12505 7977-7984 JJ denotes optical
T12506 7985-7992 NN denotes density
T12507 7993-7995 IN denotes of
T12508 7996-7999 CD denotes 0.5
T12509 8000-8001 -LRB- denotes (
T12510 8001-8002 NN denotes λ
T12511 8003-8004 SYM denotes =
T12512 8005-8008 CD denotes 600
T12513 8009-8011 NN denotes nm
T12514 8011-8012 -RRB- denotes )
T12515 8012-8013 . denotes .
T12516 8014-8016 TO denotes To
T12517 8017-8024 VB denotes prepare
T12518 8025-8036 JJ denotes heat-killed
T12519 8037-8045 NNS denotes bacteria
T12520 8045-8046 , denotes ,
T12521 8047-8051 VBP denotes live
T12522 8052-8056 CD denotes PAO1
T12523 8057-8060 VBD denotes was
T12524 8061-8072 VBN denotes resuspended
T12525 8073-8075 IN denotes in
T12526 8076-8079 NNP denotes PBS
T12527 8080-8082 TO denotes to
T12528 8083-8085 DT denotes an
T12529 8086-8093 JJ denotes optical
T12530 8094-8101 NN denotes density
T12531 8102-8104 IN denotes of
T12532 8105-8108 CD denotes 0.5
T12533 8109-8112 CC denotes and
T12534 8113-8119 VBN denotes heated
T12535 8120-8122 IN denotes at
T12536 8123-8125 CD denotes 60
T12537 8125-8126 CD denotes °
T12538 8126-8127 NNP denotes C
T12539 8128-8131 IN denotes for
T12540 8132-8133 CD denotes 1
T12541 8134-8138 NN denotes hour
T12542 8138-8139 . denotes .
T12543 8140-8143 IN denotes For
T12544 8144-8156 NNS denotes stimulations
T12545 8156-8157 , denotes ,
T12546 8158-8162 VBP denotes live
T12547 8163-8167 CD denotes PAO1
T12548 8168-8171 VBD denotes was
T12549 8172-8183 VBN denotes resuspended
T12550 8184-8186 TO denotes to
T12551 8187-8189 DT denotes an
T12552 8190-8197 JJ denotes optical
T12553 8198-8205 NN denotes density
T12554 8206-8208 IN denotes of
T12555 8209-8212 CD denotes 0.5
T12556 8213-8215 IN denotes in
T12557 8216-8219 NNP denotes PBS
T12558 8220-8223 CC denotes and
T12559 8224-8231 JJ denotes further
T12560 8232-8239 VBN denotes diluted
T12561 8240-8242 IN denotes in
T12562 8243-8250 NN denotes culture
T12563 8251-8257 NN denotes medium
T12564 8258-8263 RB denotes prior
T12565 8264-8266 TO denotes to
T12566 8267-8278 NN denotes stimulation
T12567 8279-8281 TO denotes to
T12568 8282-8289 VB denotes achieve
T12569 8290-8293 DT denotes the
T12570 8294-8301 VBN denotes desired
T12571 8302-8314 NN denotes multiplicity
T12572 8315-8317 IN denotes of
T12573 8318-8327 NN denotes infection
T12574 8327-8328 . denotes .
T12575 8329-8340 JJ denotes Heat-killed
T12576 8341-8345 NNP denotes PAO1
T12577 8346-8349 VBD denotes was
T12578 8350-8355 VBN denotes added
T12579 8356-8358 IN denotes in
T12580 8359-8360 DT denotes a
T12581 8361-8367 NN denotes volume
T12582 8368-8378 NN denotes equivalent
T12583 8379-8381 TO denotes to
T12584 8382-8386 DT denotes that
T12585 8387-8391 VBN denotes used
T12586 8392-8394 TO denotes to
T12587 8395-8402 VB denotes achieve
T12588 8403-8405 DT denotes an
T12589 8406-8409 NNP denotes MOI
T12590 8410-8412 IN denotes of
T12591 8413-8414 CD denotes 1
T12592 8415-8418 IN denotes for
T12593 8419-8423 JJ denotes live
T12594 8424-8428 NN denotes PAO1
T12595 8428-8429 . denotes .
T12642 8431-8441 NNPS denotes Statistics
T12643 8442-8445 DT denotes All
T12644 8446-8452 NNS denotes graphs
T12645 8453-8460 VBP denotes display
T12646 8461-8464 DT denotes the
T12647 8465-8469 JJ denotes mean
T12648 8470-8471 NN denotes ±
T12649 8472-8475 NNP denotes SEM
T12650 8476-8479 CC denotes and
T12651 8480-8484 VBD denotes were
T12652 8485-8494 VBN denotes generated
T12653 8495-8499 IN denotes with
T12654 8500-8505 NNP denotes Prism
T12655 8506-8507 CD denotes 5
T12656 8508-8509 -LRB- denotes (
T12657 8509-8517 NNP denotes Graphpad
T12658 8517-8518 -RRB- denotes )
T12659 8518-8519 . denotes .
T12660 8520-8531 NNP denotes Statistical
T12661 8532-8544 NN denotes significance
T12662 8545-8548 VBD denotes was
T12663 8549-8559 VBN denotes determined
T12664 8560-8562 IN denotes by
T12665 8563-8573 VBG denotes performing
T12666 8574-8577 CD denotes one
T12667 8578-8580 CC denotes or
T12668 8581-8588 JJ denotes two-way
T12669 8589-8594 NNP denotes ANOVA
T12670 8595-8598 CC denotes and
T12671 8599-8602 DT denotes the
T12672 8603-8613 NNP denotes Bonferroni
T12673 8614-8623 JJS denotes post-test
T12674 8624-8629 WRB denotes where
T12675 8630-8640 JJ denotes applicable
T12676 8640-8641 . denotes .
R8189 T9373 T9378 nsubjpass Ethics,obtained
R8190 T9374 T9375 compound Statement,Blood
R8191 T9375 T9376 compound Blood,samples
R8192 T9376 T9378 nsubjpass samples,obtained
R8193 T9377 T9378 auxpass were,obtained
R8194 T9378 T9378 ROOT obtained,obtained
R8195 T9379 T9378 prep with,obtained
R8196 T9380 T9382 amod informed,consent
R8197 T9381 T9382 amod written,consent
R8198 T9382 T9379 pobj consent,with
R8199 T9383 T9382 prep from,consent
R8200 T9384 T9385 compound control,subjects
R8201 T9385 T9383 pobj subjects,from
R8202 T9386 T9385 cc and,subjects
R8203 T9387 T9388 compound CF,patients
R8204 T9388 T9385 conj patients,subjects
R8205 T9389 T9385 prep at,subjects
R8206 T9390 T9392 det the,Children
R8207 T9391 T9392 compound BC,Children
R8208 T9392 T9394 poss Children,Hospital
R8209 T9393 T9392 case 's,Children
R8210 T9394 T9389 pobj Hospital,at
R8211 T9395 T9378 punct .,obtained
R8212 T9396 T9398 nsubjpass Consent,obtained
R8213 T9397 T9398 auxpass was,obtained
R8214 T9398 T9398 ROOT obtained,obtained
R8215 T9399 T9398 prep for,obtained
R8216 T9400 T9399 pobj children,for
R8217 T9401 T9398 agent by,obtained
R8218 T9402 T9403 poss their,parent
R8219 T9403 T9401 pobj parent,by
R8220 T9404 T9403 cc or,parent
R8221 T9405 T9406 amod legal,guardian
R8222 T9406 T9403 conj guardian,parent
R8223 T9407 T9398 punct .,obtained
R8224 T9408 T9416 nsubjpass Subjects,required
R8225 T9409 T9410 nummod 7,years
R8226 T9410 T9408 dobj years,Subjects
R8227 T9411 T9410 prep of,years
R8228 T9412 T9411 pobj age,of
R8229 T9413 T9410 cc and,years
R8230 T9414 T9416 nsubjpass older,required
R8231 T9415 T9416 auxpass were,required
R8232 T9416 T9416 ROOT required,required
R8233 T9417 T9418 aux to,provide
R8234 T9418 T9416 xcomp provide,required
R8235 T9419 T9420 amod informed,assent
R8236 T9420 T9418 dobj assent,provide
R8237 T9421 T9422 advmod as,well
R8238 T9422 T9418 advmod well,provide
R8239 T9423 T9416 punct .,required
R8240 T9424 T9426 nsubjpass Protocols,approved
R8241 T9425 T9426 auxpass were,approved
R8242 T9426 T9426 ROOT approved,approved
R8243 T9427 T9426 agent by,approved
R8244 T9428 T9432 det the,Board
R8245 T9429 T9432 compound Clinical,Board
R8246 T9430 T9431 compound Research,Ethics
R8247 T9431 T9432 compound Ethics,Board
R8248 T9432 T9427 pobj Board,by
R8249 T9433 T9432 punct (,Board
R8250 T9434 T9432 appos H09-01192,Board
R8251 T9435 T9432 punct ),Board
R8252 T9436 T9426 punct .,approved
R8670 T9881 T9883 compound Cell,CF
R8671 T9882 T9883 compound Culture,CF
R8672 T9883 T9898 nsubjpass CF,obtained
R8673 T9884 T9883 punct (,CF
R8674 T9885 T9896 nmod IB3-1,cells
R8675 T9886 T9885 cc and,IB3-1
R8676 T9887 T9885 conj CuFi-1,IB3-1
R8677 T9888 T9887 punct ),CuFi-1
R8678 T9889 T9885 cc and,IB3-1
R8679 T9890 T9885 conj control,IB3-1
R8680 T9891 T9890 punct (,control
R8681 T9892 T9890 appos S9,control
R8682 T9893 T9885 cc and,IB3-1
R8683 T9894 T9885 conj NuLi-1,IB3-1
R8684 T9895 T9896 punct ),cells
R8685 T9896 T9898 nsubjpass cells,obtained
R8686 T9897 T9898 auxpass were,obtained
R8687 T9898 T9898 ROOT obtained,obtained
R8688 T9899 T9898 prep from,obtained
R8689 T9900 T9904 det the,Collection
R8690 T9901 T9904 compound American,Collection
R8691 T9902 T9904 compound Type,Collection
R8692 T9903 T9904 compound Culture,Collection
R8693 T9904 T9899 pobj Collection,from
R8694 T9905 T9898 punct .,obtained
R8695 T9906 T9907 nummod IB3-1,cells
R8696 T9907 T9909 nsubjpass cells,derived
R8697 T9908 T9909 auxpass were,derived
R8698 T9909 T9909 ROOT derived,derived
R8699 T9910 T9909 prep from,derived
R8700 T9911 T9912 det a,patient
R8701 T9912 T9910 pobj patient,from
R8702 T9913 T9912 acl expressing,patient
R8703 T9914 T9915 det the,ΔF508
R8704 T9915 T9913 dobj ΔF508,expressing
R8705 T9916 T9915 cc and,ΔF508
R8706 T9917 T9915 conj W1282X,ΔF508
R8707 T9918 T9915 conj mutations,ΔF508
R8708 T9919 T9918 cc and,mutations
R8709 T9920 T9918 conj CuFi-1,mutations
R8710 T9921 T9922 auxpass were,derived
R8711 T9922 T9909 conj derived,derived
R8712 T9923 T9922 prep from,derived
R8713 T9924 T9927 det a,patient
R8714 T9925 T9927 nmod ΔF508,patient
R8715 T9926 T9927 amod homozygous,patient
R8716 T9927 T9923 pobj patient,from
R8717 T9928 T9909 punct .,derived
R8718 T9929 T9930 nummod S9,cells
R8719 T9930 T9931 nsubj cells,are
R8720 T9931 T9931 ROOT are,are
R8721 T9932 T9933 amod IB3-1,cells
R8722 T9933 T9931 attr cells,are
R8723 T9934 T9937 nsubjpass that,transfected
R8724 T9935 T9937 aux have,transfected
R8725 T9936 T9937 auxpass been,transfected
R8726 T9937 T9933 relcl transfected,cells
R8727 T9938 T9937 prep with,transfected
R8728 T9939 T9940 nsubj CFTR,using
R8729 T9940 T9938 pcomp using,with
R8730 T9941 T9949 det an,derived
R8731 T9942 T9944 amod adeno-associated,vector
R8732 T9943 T9944 amod viral,vector
R8733 T9944 T9949 nsubjpass vector,derived
R8734 T9945 T9944 cc and,vector
R8735 T9946 T9944 conj NuLi-1,vector
R8736 T9947 T9944 conj cells,vector
R8737 T9948 T9949 auxpass were,derived
R8738 T9949 T9931 advcl derived,are
R8739 T9950 T9949 prep from,derived
R8740 T9951 T9952 det a,patient
R8741 T9952 T9950 pobj patient,from
R8742 T9953 T9952 acl possessing,patient
R8743 T9954 T9957 det a,genotype
R8744 T9955 T9957 amod wild-type,genotype
R8745 T9956 T9957 amod CFTR,genotype
R8746 T9957 T9953 dobj genotype,possessing
R8747 T9958 T9949 punct .,derived
R8748 T9959 T9960 amod THP1-XBlue,cells
R8749 T9960 T9962 nsubj cells,express
R8750 T9961 T9962 advmod stably,express
R8751 T9962 T9962 ROOT express,express
R8752 T9963 T9967 det a,phosphatase
R8753 T9964 T9967 amod secreted,phosphatase
R8754 T9965 T9967 amod embryonic,phosphatase
R8755 T9966 T9967 compound alkaline,phosphatase
R8756 T9967 T9962 dobj phosphatase,express
R8757 T9968 T9967 punct (,phosphatase
R8758 T9969 T9967 appos SEAP,phosphatase
R8759 T9970 T9967 punct ),phosphatase
R8760 T9971 T9972 compound reporter,inducible
R8761 T9972 T9967 appos inducible,phosphatase
R8762 T9973 T9972 prep by,inducible
R8763 T9974 T9973 pobj NF-κB,by
R8764 T9975 T9974 cc and,NF-κB
R8765 T9976 T9974 conj AP-1,NF-κB
R8766 T9977 T9978 punct (,Invivogen
R8767 T9978 T9976 appos Invivogen,AP-1
R8768 T9979 T9976 punct ),AP-1
R8769 T9980 T9962 punct .,express
R8770 T9981 T9983 nsubjpass Cells,cultured
R8771 T9982 T9983 auxpass were,cultured
R8772 T9983 T9983 ROOT cultured,cultured
R8773 T9984 T9985 mark as,recommended
R8774 T9985 T9983 advcl recommended,cultured
R8775 T9986 T9985 agent by,recommended
R8776 T9987 T9989 poss their,suppliers
R8777 T9988 T9989 amod respective,suppliers
R8778 T9989 T9986 pobj suppliers,by
R8779 T9990 T9989 acl using,suppliers
R8780 T9991 T9992 amod standard,protocols
R8781 T9992 T9990 dobj protocols,using
R8782 T9993 T9983 punct .,cultured
R8783 T9994 T9997 nummod S9,cells
R8784 T9995 T9994 cc and,S9
R8785 T9996 T9994 conj IB3-1,S9
R8786 T9997 T9999 nsubjpass cells,cultured
R8787 T9998 T9999 auxpass were,cultured
R8788 T9999 T9999 ROOT cultured,cultured
R8789 T10000 T9999 prep in,cultured
R8790 T10001 T10002 amod basal,LHC-8
R8791 T10002 T10000 pobj LHC-8,in
R8792 T10003 T10004 punct (,Invitrogen
R8793 T10004 T10002 appos Invitrogen,LHC-8
R8794 T10005 T9999 punct ),cultured
R8795 T10006 T9999 prep supplemented,cultured
R8796 T10007 T10006 prep with,supplemented
R8797 T10008 T10009 nummod 10,%
R8798 T10009 T10007 pobj %,with
R8799 T10010 T10011 punct (,v/v
R8800 T10011 T10009 appos v/v,%
R8801 T10012 T10011 punct ),v/v
R8802 T10013 T10015 amod fetal,serum
R8803 T10014 T10015 amod bovine,serum
R8804 T10015 T10009 appos serum,%
R8805 T10016 T10017 punct (,FBS
R8806 T10017 T10015 parataxis FBS,serum
R8807 T10018 T10017 punct ),FBS
R8808 T10019 T10015 punct ",",serum
R8809 T10020 T10022 nummod 2,L-glutamine
R8810 T10021 T10022 compound mM,L-glutamine
R8811 T10022 T10015 appos L-glutamine,serum
R8812 T10023 T10015 punct ",",serum
R8813 T10024 T10015 cc and,serum
R8814 T10025 T10026 nummod 1,mM
R8815 T10026 T10028 compound mM,pyruvate
R8816 T10027 T10028 compound sodium,pyruvate
R8817 T10028 T10015 conj pyruvate,serum
R8818 T10029 T9999 punct .,cultured
R8819 T10030 T10033 amod NuLi-1,cells
R8820 T10031 T10033 compound /,cells
R8821 T10032 T10033 compound CuFi-1,cells
R8822 T10033 T10034 nsubj cells,were
R8823 T10034 T10034 ROOT were,were
R8824 T10035 T10034 acomp cultured,were
R8825 T10036 T10035 prep in,cultured
R8826 T10037 T10039 nmod BEBM,medium
R8827 T10038 T10039 amod serum-free,medium
R8828 T10039 T10036 pobj medium,in
R8829 T10040 T10041 punct (,Lonza
R8830 T10041 T10039 appos Lonza,medium
R8831 T10042 T10035 punct ),cultured
R8832 T10043 T10034 prep with,were
R8833 T10044 T10046 compound supplement,kit
R8834 T10045 T10046 compound bullet,kit
R8835 T10046 T10043 pobj kit,with
R8836 T10047 T10046 punct (,kit
R8837 T10048 T10050 nmod EGF,hydrocortisone
R8838 T10049 T10050 punct ",",hydrocortisone
R8839 T10050 T10046 appos hydrocortisone,kit
R8840 T10051 T10050 punct ",",hydrocortisone
R8841 T10052 T10054 amod bovine,extract
R8842 T10053 T10054 amod pituitary,extract
R8843 T10054 T10050 conj extract,hydrocortisone
R8844 T10055 T10054 punct ",",extract
R8845 T10056 T10050 conj transferrin,hydrocortisone
R8846 T10057 T10056 punct ",",transferrin
R8847 T10058 T10059 amod bovine,insulin
R8848 T10059 T10056 conj insulin,transferrin
R8849 T10060 T10059 punct ",",insulin
R8850 T10061 T10059 conj triiodothyronine,insulin
R8851 T10062 T10061 punct ",",triiodothyronine
R8852 T10063 T10061 conj epinephrine,triiodothyronine
R8853 T10064 T10063 punct ",",epinephrine
R8854 T10065 T10066 amod retinoic,acid
R8855 T10066 T10063 appos acid,epinephrine
R8856 T10067 T10063 punct ),epinephrine
R8857 T10068 T10061 punct ",",triiodothyronine
R8858 T10069 T10071 nummod 2,L-glutamine
R8859 T10070 T10071 compound mM,L-glutamine
R8860 T10071 T10061 conj L-glutamine,triiodothyronine
R8861 T10072 T10071 punct ",",L-glutamine
R8862 T10073 T10071 cc and,L-glutamine
R8863 T10074 T10077 nummod 1,pyruvate
R8864 T10075 T10077 compound mM,pyruvate
R8865 T10076 T10077 compound sodium,pyruvate
R8866 T10077 T10071 conj pyruvate,L-glutamine
R8867 T10078 T10077 punct .,pyruvate
R8868 T10079 T10085 nsubj PBMCs,were
R8869 T10080 T10079 prep from,PBMCs
R8870 T10081 T10082 compound CF,patients
R8871 T10082 T10080 pobj patients,from
R8872 T10083 T10082 cc and,patients
R8873 T10084 T10082 conj controls,patients
R8874 T10085 T10085 ROOT were,were
R8875 T10086 T10085 acomp cultured,were
R8876 T10087 T10086 prep in,cultured
R8877 T10088 T10087 pobj RPMI-1640,in
R8878 T10089 T10088 punct (,RPMI-1640
R8879 T10090 T10088 appos Hyclone,RPMI-1640
R8880 T10091 T10088 punct ),RPMI-1640
R8881 T10092 T10086 conj supplemented,cultured
R8882 T10093 T10092 prep with,supplemented
R8883 T10094 T10095 nummod 10,%
R8884 T10095 T10096 compound %,FBS
R8885 T10096 T10093 pobj FBS,with
R8886 T10097 T10096 punct ",",FBS
R8887 T10098 T10100 nummod 2,L-glutamine
R8888 T10099 T10100 compound mM,L-glutamine
R8889 T10100 T10096 conj L-glutamine,FBS
R8890 T10101 T10100 punct ",",L-glutamine
R8891 T10102 T10100 cc and,L-glutamine
R8892 T10103 T10106 nummod 1,pyruvate
R8893 T10104 T10106 compound mM,pyruvate
R8894 T10105 T10106 compound sodium,pyruvate
R8895 T10106 T10100 conj pyruvate,L-glutamine
R8896 T10107 T10106 punct (,pyruvate
R8897 T10108 T10109 amod complete,RPMI
R8898 T10109 T10106 appos RPMI,pyruvate
R8899 T10110 T10106 punct ),pyruvate
R8900 T10111 T10085 punct .,were
R8901 T10112 T10113 amod THP1-XBlue,cells
R8902 T10113 T10114 nsubj cells,were
R8903 T10114 T10114 ROOT were,were
R8904 T10115 T10114 acomp cultured,were
R8905 T10116 T10115 prep in,cultured
R8906 T10117 T10118 amod complete,RPMI
R8907 T10118 T10116 pobj RPMI,in
R8908 T10119 T10115 prep with,cultured
R8909 T10120 T10121 det the,addition
R8910 T10121 T10119 pobj addition,with
R8911 T10122 T10121 prep of,addition
R8912 T10123 T10122 pobj zeocin,of
R8913 T10124 T10123 punct (,zeocin
R8914 T10125 T10126 nummod 100,µg
R8915 T10126 T10128 nmod µg,ml
R8916 T10127 T10128 compound /,ml
R8917 T10128 T10123 appos ml,zeocin
R8918 T10129 T10128 punct ),ml
R8919 T10130 T10131 aux to,select
R8920 T10131 T10115 advcl select,cultured
R8921 T10132 T10131 prep for,select
R8922 T10133 T10132 pobj cells,for
R8923 T10134 T10133 acl expressing,cells
R8924 T10135 T10140 det the,reporter
R8925 T10136 T10139 compound SEAP,AP-1
R8926 T10137 T10139 compound NF-κB,AP-1
R8927 T10138 T10139 compound /,AP-1
R8928 T10139 T10140 compound AP-1,reporter
R8929 T10140 T10134 dobj reporter,expressing
R8930 T10141 T10114 punct .,were
R8931 T10142 T10151 advmod Prior,plated
R8932 T10143 T10151 prep to,plated
R8933 T10144 T10143 pobj stimulation,to
R8934 T10145 T10151 punct ",",plated
R8935 T10146 T10149 amod bronchial,lines
R8936 T10147 T10149 amod epithelial,lines
R8937 T10148 T10149 compound cell,lines
R8938 T10149 T10151 nsubjpass lines,plated
R8939 T10150 T10151 auxpass were,plated
R8940 T10151 T10151 ROOT plated,plated
R8941 T10152 T10151 prep in,plated
R8942 T10153 T10158 amod coated,plates
R8943 T10154 T10158 nmod [,plates
R8944 T10155 T10158 nummod 62,plates
R8945 T10156 T10158 nmod ],plates
R8946 T10157 T10158 compound 96-well,plates
R8947 T10158 T10152 pobj plates,in
R8948 T10159 T10161 punct (,Biosciences
R8949 T10160 T10161 compound BD,Biosciences
R8950 T10161 T10158 appos Biosciences,plates
R8951 T10162 T10161 punct ),Biosciences
R8952 T10163 T10151 prep at,plated
R8953 T10164 T10167 nummod 3,cells/well
R8954 T10165 T10167 nummod ×,cells/well
R8955 T10166 T10167 nummod 104,cells/well
R8956 T10167 T10163 pobj cells/well,at
R8957 T10168 T10169 mark unless,indicated
R8958 T10169 T10151 advcl indicated,plated
R8959 T10170 T10151 punct ",",plated
R8960 T10171 T10151 cc and,plated
R8961 T10172 T10151 conj allowed,plated
R8962 T10173 T10174 aux to,adhere
R8963 T10174 T10172 xcomp adhere,allowed
R8964 T10175 T10174 advmod overnight,adhere
R8965 T10176 T10151 punct .,plated
R8966 T10177 T10184 nsubjpass Plates,coated
R8967 T10178 T10177 prep for,Plates
R8968 T10179 T10178 pobj S9,for
R8969 T10180 T10179 cc and,S9
R8970 T10181 T10179 conj IB3-1,S9
R8971 T10182 T10184 nsubjpass stimulations,coated
R8972 T10183 T10184 auxpass were,coated
R8973 T10184 T10184 ROOT coated,coated
R8974 T10185 T10184 prep in,coated
R8975 T10186 T10187 det a,mixture
R8976 T10187 T10185 pobj mixture,in
R8977 T10188 T10187 prep of,mixture
R8978 T10189 T10190 amod bovine,serum
R8979 T10190 T10191 compound serum,albumin
R8980 T10191 T10188 pobj albumin,of
R8981 T10192 T10191 punct (,albumin
R8982 T10193 T10194 nummod 100,µg
R8983 T10194 T10196 compound µg,ml
R8984 T10195 T10196 compound /,ml
R8985 T10196 T10191 appos ml,albumin
R8986 T10197 T10191 punct ),albumin
R8987 T10198 T10184 punct ",",coated
R8988 T10199 T10184 dep fibronectin,coated
R8989 T10200 T10199 punct (,fibronectin
R8990 T10201 T10202 nummod 10,µg
R8991 T10202 T10199 appos µg,fibronectin
R8992 T10203 T10204 compound /,ml
R8993 T10204 T10202 appos ml,µg
R8994 T10205 T10202 punct ),µg
R8995 T10206 T10199 punct ",",fibronectin
R8996 T10207 T10199 cc and,fibronectin
R8997 T10208 T10209 amod bovine,collage
R8998 T10209 T10210 compound collage,type
R8999 T10210 T10199 conj type,fibronectin
R9000 T10211 T10199 nsubj I,fibronectin
R9001 T10212 T10211 punct (,I
R9002 T10213 T10214 nummod 30,µg
R9003 T10214 T10216 compound µg,ml
R9004 T10215 T10216 compound /,ml
R9005 T10216 T10211 appos ml,I
R9006 T10217 T10211 punct ),I
R9007 T10218 T10220 punct (,Biosciences
R9008 T10219 T10220 compound BD,Biosciences
R9009 T10220 T10211 appos Biosciences,I
R9010 T10221 T10220 punct ),Biosciences
R9011 T10222 T10184 punct .,coated
R9012 T10223 T10229 nsubjpass Plates,coated
R9013 T10224 T10223 prep for,Plates
R9014 T10225 T10224 pobj NuLi-1,for
R9015 T10226 T10225 cc and,NuLi-1
R9016 T10227 T10225 conj CuFi-1,NuLi-1
R9017 T10228 T10229 auxpass were,coated
R9018 T10229 T10229 ROOT coated,coated
R9019 T10230 T10229 prep with,coated
R9020 T10231 T10232 compound collagen,type
R9021 T10232 T10233 compound type,IV
R9022 T10233 T10230 pobj IV,with
R9023 T10234 T10233 punct (,IV
R9024 T10235 T10236 nummod 60,µg
R9025 T10236 T10238 compound µg,ml
R9026 T10237 T10238 compound /,ml
R9027 T10238 T10233 appos ml,IV
R9028 T10239 T10233 punct ),IV
R9029 T10240 T10233 punct (,IV
R9030 T10241 T10242 compound Sigma,Aldrich
R9031 T10242 T10233 appos Aldrich,IV
R9032 T10243 T10233 punct ),IV
R9033 T10244 T10229 punct .,coated
R9034 T10245 T10247 nsubjpass PBMCs,plated
R9035 T10246 T10247 auxpass were,plated
R9036 T10247 T10247 ROOT plated,plated
R9037 T10248 T10247 prep in,plated
R9038 T10249 T10250 amod 96-well,plates
R9039 T10250 T10248 pobj plates,in
R9040 T10251 T10247 prep at,plated
R9041 T10252 T10253 det a,density
R9042 T10253 T10251 pobj density,at
R9043 T10254 T10253 prep of,density
R9044 T10255 T10258 nummod 1.5,cells/well
R9045 T10256 T10258 nmod ×,cells/well
R9046 T10257 T10258 nummod 105,cells/well
R9047 T10258 T10254 pobj cells/well,of
R9048 T10259 T10247 prep in,plated
R9049 T10260 T10261 nummod 200,µl
R9050 T10261 T10259 pobj µl,in
R9051 T10262 T10261 punct (,µl
R9052 T10263 T10266 nummod 7.5,cells/mL
R9053 T10264 T10266 nummod ×,cells/mL
R9054 T10265 T10266 nummod 105,cells/mL
R9055 T10266 T10261 appos cells/mL,µl
R9056 T10267 T10261 punct ),µl
R9057 T10268 T10247 punct .,plated
R9058 T10269 T10271 nummod THP-1,cells
R9059 T10270 T10271 compound reporter,cells
R9060 T10271 T10273 nsubjpass cells,differentiated
R9061 T10272 T10273 auxpass were,differentiated
R9062 T10273 T10273 ROOT differentiated,differentiated
R9063 T10274 T10273 prep into,differentiated
R9064 T10275 T10277 det a,phenotype
R9065 T10276 T10277 amod macrophage-like,phenotype
R9066 T10277 T10274 pobj phenotype,into
R9067 T10278 T10273 advcl using,differentiated
R9068 T10279 T10280 nummod 50,ng/ml
R9069 T10280 T10278 dobj ng/ml,using
R9070 T10281 T10280 prep of,ng/ml
R9071 T10282 T10284 nmod phorbol,13-acetate
R9072 T10283 T10284 amod 12-myristate,13-acetate
R9073 T10284 T10281 pobj 13-acetate,of
R9074 T10285 T10286 punct (,PMA
R9075 T10286 T10284 appos PMA,13-acetate
R9076 T10287 T10284 punct ),13-acetate
R9077 T10288 T10290 punct (,Aldrich
R9078 T10289 T10290 compound Sigma,Aldrich
R9079 T10290 T10284 appos Aldrich,13-acetate
R9080 T10291 T10280 punct ),ng/ml
R9081 T10292 T10278 prep for,using
R9082 T10293 T10294 nummod 24,hours
R9083 T10294 T10292 pobj hours,for
R9084 T10295 T10278 prep at,using
R9085 T10296 T10297 det a,density
R9086 T10297 T10295 pobj density,at
R9087 T10298 T10297 prep of,density
R9088 T10299 T10302 nummod 1,cells/well
R9089 T10300 T10302 nummod ×,cells/well
R9090 T10301 T10302 nummod 105,cells/well
R9091 T10302 T10298 pobj cells/well,of
R9092 T10303 T10278 prep in,using
R9093 T10304 T10305 nummod 200,µl
R9094 T10305 T10303 pobj µl,in
R9095 T10306 T10305 punct (,µl
R9096 T10307 T10310 nummod 5,cells/ml
R9097 T10308 T10310 nummod ×,cells/ml
R9098 T10309 T10310 nummod 105,cells/ml
R9099 T10310 T10305 appos cells/ml,µl
R9100 T10311 T10305 punct ),µl
R9101 T10312 T10273 punct .,differentiated
R9102 T10313 T10315 nsubjpass Cells,washed
R9103 T10314 T10315 auxpass were,washed
R9104 T10315 T10315 ROOT washed,washed
R9105 T10316 T10315 prep with,washed
R9106 T10317 T10316 pobj PBS,with
R9107 T10318 T10315 cc and,washed
R9108 T10319 T10315 conj allowed,washed
R9109 T10320 T10321 aux to,rest
R9110 T10321 T10319 xcomp rest,allowed
R9111 T10322 T10326 det a,prior
R9112 T10323 T10326 amod further,prior
R9113 T10324 T10325 nummod 42,hours
R9114 T10325 T10326 npadvmod hours,prior
R9115 T10326 T10321 advmod prior,rest
R9116 T10327 T10326 prep to,prior
R9117 T10328 T10327 pobj stimulation,to
R9118 T10329 T10315 punct .,washed
R9348 T10630 T10640 nsubjpass CF,established
R9349 T10631 T10630 cc and,CF
R9350 T10632 T10633 nsubj control,subject
R9351 T10633 T10640 advcl subject,established
R9352 T10634 T10633 dobj PBMCs,subject
R9353 T10635 T10636 det The,diagnosis
R9354 T10636 T10640 nsubjpass diagnosis,established
R9355 T10637 T10636 prep of,diagnosis
R9356 T10638 T10637 pobj CF,of
R9357 T10639 T10640 auxpass was,established
R9358 T10640 T10640 ROOT established,established
R9359 T10641 T10640 agent by,established
R9360 T10642 T10644 amod typical,features
R9361 T10643 T10644 amod clinical,features
R9362 T10644 T10641 pobj features,by
R9363 T10645 T10640 punct ",",established
R9364 T10646 T10640 dep increased,established
R9365 T10647 T10648 compound sweat,chloride
R9366 T10648 T10649 compound chloride,concentrations
R9367 T10649 T10646 dobj concentrations,increased
R9368 T10650 T10649 punct (,concentrations
R9369 T10651 T10649 appos >,concentrations
R9370 T10652 T10653 nummod 60,mmol/l
R9371 T10653 T10651 appos mmol/l,>
R9372 T10654 T10653 punct ),mmol/l
R9373 T10655 T10651 punct ",",>
R9374 T10656 T10651 cc and,>
R9375 T10657 T10651 conj detection,>
R9376 T10658 T10657 prep of,detection
R9377 T10659 T10660 amod CF-inducing,mutations
R9378 T10660 T10658 pobj mutations,of
R9379 T10661 T10640 punct .,established
R9380 T10662 T10663 det All,patients
R9381 T10663 T10666 nsubj patients,were
R9382 T10664 T10663 prep with,patients
R9383 T10665 T10664 pobj CF,with
R9384 T10666 T10666 ROOT were,were
R9385 T10667 T10668 advmod clinically,stable
R9386 T10668 T10666 acomp stable,were
R9387 T10669 T10666 prep at,were
R9388 T10670 T10671 det the,time
R9389 T10671 T10669 pobj time,at
R9390 T10672 T10671 prep of,time
R9391 T10673 T10674 compound blood,donation
R9392 T10674 T10672 pobj donation,of
R9393 T10675 T10666 punct ",",were
R9394 T10676 T10666 cc and,were
R9395 T10677 T10678 nsubj we,excluded
R9396 T10678 T10666 conj excluded,were
R9397 T10679 T10680 det any,subjects
R9398 T10680 T10678 dobj subjects,excluded
R9399 T10681 T10683 nsubj who,receiving
R9400 T10682 T10683 aux were,receiving
R9401 T10683 T10680 relcl receiving,subjects
R9402 T10684 T10685 amod systemic,corticosteroids
R9403 T10685 T10683 dobj corticosteroids,receiving
R9404 T10686 T10680 amod due,subjects
R9405 T10687 T10686 prep to,due
R9406 T10688 T10690 amod potential,activity
R9407 T10689 T10690 amod immunomodulatory,activity
R9408 T10690 T10687 pobj activity,to
R9409 T10691 T10678 punct .,excluded
R9410 T10692 T10693 compound Control,samples
R9411 T10693 T10695 nsubjpass samples,provided
R9412 T10694 T10695 auxpass were,provided
R9413 T10695 T10695 ROOT provided,provided
R9414 T10696 T10695 agent by,provided
R9415 T10697 T10699 amod healthy,volunteers
R9416 T10698 T10699 compound adult,volunteers
R9417 T10699 T10696 pobj volunteers,by
R9418 T10700 T10695 punct .,provided
R9419 T10701 T10707 prep In,demonstrated
R9420 T10702 T10703 advmod previously,published
R9421 T10703 T10704 amod published,work
R9422 T10704 T10701 pobj work,In
R9423 T10705 T10707 nsubj we,demonstrated
R9424 T10706 T10707 aux have,demonstrated
R9425 T10707 T10729 ccomp demonstrated,age-match
R9426 T10708 T10712 mark that,are
R9427 T10709 T10711 amod TLR-mediated,responses
R9428 T10710 T10711 amod inflammatory,responses
R9429 T10711 T10712 nsubj responses,are
R9430 T10712 T10707 ccomp are,demonstrated
R9431 T10713 T10712 acomp stable,are
R9432 T10714 T10713 prep in,stable
R9433 T10715 T10714 pobj humans,in
R9434 T10716 T10712 prep from,are
R9435 T10717 T10716 pobj birth,from
R9436 T10718 T10712 prep to,are
R9437 T10719 T10720 nummod 60,years
R9438 T10720 T10721 npadvmod years,old
R9439 T10721 T10718 amod old,to
R9440 T10722 T10724 nmod [,]
R9441 T10723 T10724 nummod 63,]
R9442 T10724 T10718 pobj ],to
R9443 T10725 T10729 punct ",",age-match
R9444 T10726 T10729 advmod therefore,age-match
R9445 T10727 T10729 aux did,age-match
R9446 T10728 T10729 neg not,age-match
R9447 T10729 T10729 ROOT age-match,age-match
R9448 T10730 T10732 det the,patients
R9449 T10731 T10732 compound CF,patients
R9450 T10732 T10729 dobj patients,age-match
R9451 T10733 T10732 cc and,patients
R9452 T10734 T10735 compound control,subjects
R9453 T10735 T10732 conj subjects,patients
R9454 T10736 T10729 punct .,age-match
R9455 T10737 T10738 amod Peripheral,blood
R9456 T10738 T10740 nsubjpass blood,collected
R9457 T10739 T10740 auxpass was,collected
R9458 T10740 T10740 ROOT collected,collected
R9459 T10741 T10740 prep in,collected
R9460 T10742 T10743 compound sodium,heparin
R9461 T10743 T10744 compound heparin,tubes
R9462 T10744 T10741 pobj tubes,in
R9463 T10745 T10747 punct (,Biosciences
R9464 T10746 T10747 compound BD,Biosciences
R9465 T10747 T10744 appos Biosciences,tubes
R9466 T10748 T10747 punct ),Biosciences
R9467 T10749 T10747 cc and,Biosciences
R9468 T10750 T10747 conj PBMCs,Biosciences
R9469 T10751 T10740 auxpass were,collected
R9470 T10752 T10751 acomp isolated,were
R9471 T10753 T10752 advcl using,isolated
R9472 T10754 T10756 nmod density,centrifugation
R9473 T10755 T10756 compound gradient,centrifugation
R9474 T10756 T10753 dobj centrifugation,using
R9475 T10757 T10756 prep on,centrifugation
R9476 T10758 T10760 compound Ficoll-Paque,Plus
R9477 T10759 T10760 compound ™,Plus
R9478 T10760 T10757 pobj Plus,on
R9479 T10761 T10760 punct (,Plus
R9480 T10762 T10763 compound GE,Healthcare
R9481 T10763 T10760 appos Healthcare,Plus
R9482 T10764 T10760 punct ),Plus
R9483 T10765 T10740 punct .,collected
R9484 T10766 T10767 det The,layer
R9485 T10767 T10770 nsubj layer,was
R9486 T10768 T10767 acl containing,layer
R9487 T10769 T10768 dobj PBMCs,containing
R9488 T10770 T10770 ROOT was,was
R9489 T10771 T10770 acomp isolated,was
R9490 T10772 T10771 punct ",",isolated
R9491 T10773 T10771 conj washed,isolated
R9492 T10774 T10773 advmod twice,washed
R9493 T10775 T10773 prep in,washed
R9494 T10776 T10775 pobj PBS,in
R9495 T10777 T10773 cc and,washed
R9496 T10778 T10773 conj resuspended,washed
R9497 T10779 T10778 prep in,resuspended
R9498 T10780 T10781 amod complete,RPMI
R9499 T10781 T10779 pobj RPMI,in
R9500 T10782 T10770 punct .,was
R9501 T10783 T10785 nsubjpass Cells,enumerated
R9502 T10784 T10785 auxpass were,enumerated
R9503 T10785 T10785 ROOT enumerated,enumerated
R9504 T10786 T10785 agent by,enumerated
R9505 T10787 T10789 amod trypan,exclusion
R9506 T10788 T10789 amod blue,exclusion
R9507 T10789 T10786 pobj exclusion,by
R9508 T10790 T10785 advcl using,enumerated
R9509 T10791 T10795 det the,counter
R9510 T10792 T10795 nmod Countess,counter
R9511 T10793 T10795 amod automated,counter
R9512 T10794 T10795 compound cell,counter
R9513 T10795 T10790 dobj counter,using
R9514 T10796 T10795 punct (,counter
R9515 T10797 T10795 appos Invitrogen,counter
R9516 T10798 T10795 punct ),counter
R9517 T10799 T10785 punct .,enumerated
R9518 T10800 T10809 prep For,allowed
R9519 T10801 T10800 pobj derivation,For
R9520 T10802 T10801 prep of,derivation
R9521 T10803 T10802 pobj macrophages,of
R9522 T10804 T10801 prep from,derivation
R9523 T10805 T10804 pobj monocytes,from
R9524 T10806 T10809 punct ",",allowed
R9525 T10807 T10809 nsubjpass monocytes,allowed
R9526 T10808 T10809 auxpass were,allowed
R9527 T10809 T10809 ROOT allowed,allowed
R9528 T10810 T10811 aux to,adhere
R9529 T10811 T10809 xcomp adhere,allowed
R9530 T10812 T10811 prep to,adhere
R9531 T10813 T10812 pobj plastic,to
R9532 T10814 T10811 prep for,adhere
R9533 T10815 T10816 nummod 2,hours
R9534 T10816 T10814 pobj hours,for
R9535 T10817 T10811 prep in,adhere
R9536 T10818 T10817 pobj RPMI,in
R9537 T10819 T10825 dep 1640,removed
R9538 T10820 T10825 prep after,removed
R9539 T10821 T10820 pobj which,after
R9540 T10822 T10823 amod non-adherent,cells
R9541 T10823 T10825 nsubjpass cells,removed
R9542 T10824 T10825 auxpass were,removed
R9543 T10825 T10809 advcl removed,allowed
R9544 T10826 T10809 punct .,allowed
R9545 T10827 T10829 nsubjpass Monocytes,allowed
R9546 T10828 T10829 auxpass were,allowed
R9547 T10829 T10829 ROOT allowed,allowed
R9548 T10830 T10831 aux to,differentiate
R9549 T10831 T10829 xcomp differentiate,allowed
R9550 T10832 T10831 prep in,differentiate
R9551 T10833 T10834 compound RPMI,1640
R9552 T10834 T10832 pobj 1640,in
R9553 T10835 T10829 conj supplemented,allowed
R9554 T10836 T10835 prep with,supplemented
R9555 T10837 T10838 nummod 10,%
R9556 T10838 T10841 compound %,serum
R9557 T10839 T10840 compound human,AB
R9558 T10840 T10841 compound AB,serum
R9559 T10841 T10836 pobj serum,with
R9560 T10842 T10835 prep for,supplemented
R9561 T10843 T10844 nummod 10,days
R9562 T10844 T10842 pobj days,for
R9563 T10845 T10829 punct .,allowed
R9818 T11117 T11115 xcomp adhere,allowed
R9805 T11104 T11105 compound Cell,stimulation
R9806 T11105 T11113 nsubjpass stimulation,plated
R9807 T11106 T11105 cc and,stimulation
R9808 T11107 T11111 nmod cytokine,cells
R9809 T11108 T11111 nmod quantification,cells
R9810 T11109 T11111 nmod Bronchial,cells
R9811 T11110 T11111 amod epithelial,cells
R9812 T11111 T11105 conj cells,stimulation
R9813 T11112 T11113 auxpass were,plated
R9814 T11113 T11113 ROOT plated,plated
R9815 T11114 T11113 cc and,plated
R9816 T11115 T11113 conj allowed,plated
R9817 T11116 T11117 aux to,adhere
R9819 T11118 T11119 advmod overnight,prior
R9820 T11119 T11117 advmod prior,adhere
R9821 T11120 T11119 prep to,prior
R9822 T11121 T11120 pobj stimulation,to
R9823 T11122 T11113 punct .,plated
R9824 T11123 T11125 amod Bronchial,cells
R9825 T11124 T11125 amod epithelial,cells
R9826 T11125 T11127 nsubjpass cells,rested
R9827 T11126 T11127 auxpass were,rested
R9828 T11127 T11127 ROOT rested,rested
R9829 T11128 T11127 cc or,rested
R9830 T11129 T11127 conj primed,rested
R9831 T11130 T11129 prep with,primed
R9832 T11131 T11130 pobj LPS,with
R9833 T11132 T11129 prep for,primed
R9834 T11133 T11134 nummod 5,hours
R9835 T11134 T11132 pobj hours,for
R9836 T11135 T11129 cc and,primed
R9837 T11136 T11129 conj stimulated,primed
R9838 T11137 T11136 prep with,stimulated
R9839 T11138 T11141 amod live,PAO1
R9840 T11139 T11141 compound P.,PAO1
R9841 T11140 T11141 compound aeruginosa,PAO1
R9842 T11141 T11137 pobj PAO1,with
R9843 T11142 T11141 cc or,PAO1
R9844 T11143 T11141 conj ATP,PAO1
R9845 T11144 T11136 prep for,stimulated
R9846 T11145 T11146 det the,times
R9847 T11146 T11144 pobj times,for
R9848 T11147 T11127 conj indicated,rested
R9849 T11148 T11127 punct .,rested
R9850 T11149 T11156 nsubjpass PBMCs,rested
R9851 T11150 T11149 cc and,PBMCs
R9852 T11151 T11153 nummod THP-1,cells
R9853 T11152 T11153 compound reporter,cells
R9854 T11153 T11149 conj cells,PBMCs
R9855 T11154 T11156 auxpass were,rested
R9856 T11155 T11156 preconj either,rested
R9857 T11156 T11156 ROOT rested,rested
R9858 T11157 T11156 cc or,rested
R9859 T11158 T11156 conj primed,rested
R9860 T11159 T11158 prep with,primed
R9861 T11160 T11159 pobj LPS,with
R9862 T11161 T11160 punct (,LPS
R9863 T11162 T11160 appos Invivogen,LPS
R9864 T11163 T11160 punct ),LPS
R9865 T11164 T11158 cc or,primed
R9866 T11165 T11167 amod heat-killed,overnight
R9867 T11166 T11167 compound PAO1,overnight
R9868 T11167 T11158 conj overnight,primed
R9869 T11168 T11167 punct (,overnight
R9870 T11169 T11170 nummod 16,hours
R9871 T11170 T11167 appos hours,overnight
R9872 T11171 T11167 punct ),overnight
R9873 T11172 T11156 punct .,rested
R9874 T11173 T11175 det The,day
R9875 T11174 T11175 amod next,day
R9876 T11175 T11179 npadvmod day,challenged
R9877 T11176 T11177 det the,cells
R9878 T11177 T11179 nsubjpass cells,challenged
R9879 T11178 T11179 auxpass were,challenged
R9880 T11179 T11179 ROOT challenged,challenged
R9881 T11180 T11179 prep with,challenged
R9882 T11181 T11182 amod live,PAO1
R9883 T11182 T11180 pobj PAO1,with
R9884 T11183 T11182 punct ",",PAO1
R9885 T11184 T11182 conj PAO1ΔexsA,PAO1
R9886 T11185 T11184 punct ",",PAO1ΔexsA
R9887 T11186 T11184 conj ATP,PAO1ΔexsA
R9888 T11187 T11188 punct (,Invivogen
R9889 T11188 T11186 appos Invivogen,ATP
R9890 T11189 T11188 punct ),Invivogen
R9891 T11190 T11186 punct ",",ATP
R9892 T11191 T11186 cc or,ATP
R9893 T11192 T11186 conj Poly,ATP
R9894 T11193 T11192 punct (,Poly
R9895 T11194 T11192 appos dA,Poly
R9896 T11195 T11194 punct :,dA
R9897 T11196 T11194 nmod dT,dA
R9898 T11197 T11194 punct ),dA
R9899 T11198 T11200 punct (,Aldrich
R9900 T11199 T11200 compound Sigma,Aldrich
R9901 T11200 T11194 appos Aldrich,dA
R9902 T11201 T11192 punct ),Poly
R9903 T11202 T11179 prep for,challenged
R9904 T11203 T11204 det the,times
R9905 T11204 T11202 pobj times,for
R9906 T11205 T11179 conj indicated,challenged
R9907 T11206 T11207 punct (,see
R9908 T11207 T11205 parataxis see,indicated
R9909 T11208 T11207 dobj Fig.,see
R9910 T11209 T11208 nummod 1,Fig.
R9911 T11210 T11207 punct ),see
R9912 T11211 T11179 punct .,challenged
R9913 T11212 T11225 prep For,used
R9914 T11213 T11212 pobj stimulations,For
R9915 T11214 T11213 prep with,stimulations
R9916 T11215 T11214 pobj Poly,with
R9917 T11216 T11215 punct (,Poly
R9918 T11217 T11215 appos dA,Poly
R9919 T11218 T11215 punct :,Poly
R9920 T11219 T11215 appos dT,Poly
R9921 T11220 T11215 punct ),Poly
R9922 T11221 T11225 punct ",",used
R9923 T11222 T11223 compound lipofectamine,LTX
R9924 T11223 T11225 nsubjpass LTX,used
R9925 T11224 T11225 auxpass was,used
R9926 T11225 T11225 ROOT used,used
R9927 T11226 T11225 prep at,used
R9928 T11227 T11234 det a,v
R9929 T11228 T11234 nummod 1,v
R9930 T11229 T11234 nmod ∶,v
R9931 T11230 T11229 nummod 1,∶
R9932 T11231 T11234 punct (,v
R9933 T11232 T11234 nmod w,v
R9934 T11233 T11234 nmod ∶,v
R9935 T11234 T11226 pobj v,at
R9936 T11235 T11234 punct ),v
R9937 T11236 T11225 dobj ratio,used
R9938 T11237 T11236 prep of,ratio
R9939 T11238 T11237 pobj µg,of
R9940 T11239 T11238 prep of,µg
R9941 T11240 T11239 pobj DNA,of
R9942 T11241 T11225 prep to,used
R9943 T11242 T11241 pobj µl,to
R9944 T11243 T11242 prep of,µl
R9945 T11244 T11243 pobj lipofectamine,of
R9946 T11245 T11225 cc and,used
R9947 T11246 T11225 conj was,used
R9948 T11247 T11246 acomp mixed,was
R9949 T11248 T11249 nummod 30,minutes
R9950 T11249 T11250 npadvmod minutes,prior
R9951 T11250 T11246 advmod prior,was
R9952 T11251 T11250 prep to,prior
R9953 T11252 T11251 pobj stimulation,to
R9954 T11253 T11225 punct .,used
R9955 T11254 T11257 det The,Bay11-7082
R9956 T11255 T11257 amod NF-κB,Bay11-7082
R9957 T11256 T11257 compound inhibitor,Bay11-7082
R9958 T11257 T11262 nsubjpass Bay11-7082,added
R9959 T11258 T11259 punct (,Invivogen
R9960 T11259 T11257 appos Invivogen,Bay11-7082
R9961 T11260 T11259 punct ),Invivogen
R9962 T11261 T11262 auxpass was,added
R9963 T11262 T11262 ROOT added,added
R9964 T11263 T11262 prep to,added
R9965 T11264 T11263 pobj cultures,to
R9966 T11265 T11266 nummod 1,hour
R9967 T11266 T11267 npadvmod hour,prior
R9968 T11267 T11262 advmod prior,added
R9969 T11268 T11267 prep to,prior
R9970 T11269 T11268 pobj priming,to
R9971 T11270 T11262 punct .,added
R9972 T11271 T11275 mark If,involved
R9973 T11272 T11273 det no,priming
R9974 T11273 T11275 nsubjpass priming,involved
R9975 T11274 T11275 auxpass was,involved
R9976 T11275 T11279 advcl involved,added
R9977 T11276 T11279 punct ",",added
R9978 T11277 T11279 nsubjpass inhibitor,added
R9979 T11278 T11279 auxpass was,added
R9980 T11279 T11279 ROOT added,added
R9981 T11280 T11281 nummod 1,hour
R9982 T11281 T11282 npadvmod hour,prior
R9983 T11282 T11279 advmod prior,added
R9984 T11283 T11279 prep to,added
R9985 T11284 T11285 amod inflammasome,stimulation
R9986 T11285 T11283 pobj stimulation,to
R9987 T11286 T11279 punct .,added
R9988 T11287 T11289 det The,inhibitor
R9989 T11288 T11289 amod CFTR,inhibitor
R9990 T11289 T11296 nsubjpass inhibitor,added
R9991 T11290 T11289 appos CFTRinh172,inhibitor
R9992 T11291 T11293 punct (,Aldrich
R9993 T11292 T11293 compound Sigma,Aldrich
R9994 T11293 T11290 appos Aldrich,CFTRinh172
R9995 T11294 T11290 punct ),CFTRinh172
R9996 T11295 T11296 auxpass was,added
R9997 T11296 T11296 ROOT added,added
R9998 T11297 T11296 prep to,added
R9999 T11298 T11297 pobj cultures,to
R10000 T11299 T11300 nummod 18,hours
R10001 T11300 T11301 npadvmod hours,prior
R10002 T11301 T11296 advmod prior,added
R10003 T11302 T11301 prep to,prior
R10004 T11303 T11304 amod inflammasome,stimulation
R10005 T11304 T11302 pobj stimulation,to
R10006 T11305 T11296 punct .,added
R10007 T11306 T11308 det The,inhibitor
R10008 T11307 T11308 amod caspase-1,inhibitor
R10009 T11308 T11314 nsubjpass inhibitor,added
R10010 T11309 T11308 appos z-YVAD-fmk,inhibitor
R10011 T11310 T11311 punct (,Biovision
R10012 T11311 T11309 appos Biovision,z-YVAD-fmk
R10013 T11312 T11309 punct ),z-YVAD-fmk
R10014 T11313 T11314 auxpass was,added
R10015 T11314 T11314 ROOT added,added
R10016 T11315 T11314 prep to,added
R10017 T11316 T11315 pobj cultures,to
R10018 T11317 T11318 nummod 1,hour
R10019 T11318 T11319 npadvmod hour,prior
R10020 T11319 T11314 advmod prior,added
R10021 T11320 T11319 prep to,prior
R10022 T11321 T11322 amod inflammasome,stimulation
R10023 T11322 T11320 pobj stimulation,to
R10024 T11323 T11314 punct .,added
R10025 T11324 T11326 nsubjpass Supernatants,collected
R10026 T11325 T11326 auxpass were,collected
R10027 T11326 T11326 ROOT collected,collected
R10028 T11327 T11326 cc and,collected
R10029 T11328 T11326 conj stored,collected
R10030 T11329 T11328 prep at,stored
R10031 T11330 T11334 nummod −,Cytokines
R10032 T11331 T11332 nummod 20,°
R10033 T11332 T11334 compound °,Cytokines
R10034 T11333 T11334 compound C.,Cytokines
R10035 T11334 T11329 pobj Cytokines,at
R10036 T11335 T11326 conj released,collected
R10037 T11336 T11335 prep into,released
R10038 T11337 T11336 pobj supernatants,into
R10039 T11338 T11337 prep from,supernatants
R10040 T11339 T11338 pobj PBMCs,from
R10041 T11340 T11345 nsubjpass stimulated,quantified
R10042 T11341 T11340 prep with,stimulated
R10043 T11342 T11343 amod inflammasome,activators
R10044 T11343 T11341 pobj activators,with
R10045 T11344 T11345 auxpass were,quantified
R10046 T11345 T11326 conj quantified,collected
R10047 T11346 T11345 advcl using,quantified
R10048 T11347 T11348 compound sandwich,ELISA
R10049 T11348 T11346 dobj ELISA,using
R10050 T11349 T11348 punct (,ELISA
R10051 T11350 T11348 appos eBioscience,ELISA
R10052 T11351 T11348 punct ),ELISA
R10053 T11352 T11345 punct .,quantified
R10220 T11553 T11559 advcl Immunoblotting,seeded
R10221 T11554 T11557 nummod 1,cells
R10222 T11555 T11557 nummod ×,cells
R10223 T11556 T11557 nummod 106,cells
R10224 T11557 T11559 nsubjpass cells,seeded
R10225 T11558 T11559 auxpass were,seeded
R10226 T11559 T11559 ROOT seeded,seeded
R10227 T11560 T11559 prep in,seeded
R10228 T11561 T11562 amod 12-well,plates
R10229 T11562 T11560 pobj plates,in
R10230 T11563 T11559 punct ",",seeded
R10231 T11564 T11559 conj stimulated,seeded
R10232 T11565 T11566 mark as,indicated
R10233 T11566 T11564 advcl indicated,stimulated
R10234 T11567 T11564 punct ",",stimulated
R10235 T11568 T11564 cc and,stimulated
R10236 T11569 T11564 conj lysed,stimulated
R10237 T11570 T11569 prep in,lysed
R10238 T11571 T11572 compound RIPA,buffer
R10239 T11572 T11570 pobj buffer,in
R10240 T11573 T11569 dep supplemented,lysed
R10241 T11574 T11573 prep with,supplemented
R10242 T11575 T11576 compound Halt,protease
R10243 T11576 T11574 pobj protease,with
R10244 T11577 T11576 cc and,protease
R10245 T11578 T11580 compound phosphatase,cocktail
R10246 T11579 T11580 compound inhibitor,cocktail
R10247 T11580 T11576 conj cocktail,protease
R10248 T11581 T11583 punct (,Scientific
R10249 T11582 T11583 compound Thermo,Scientific
R10250 T11583 T11580 appos Scientific,cocktail
R10251 T11584 T11583 punct ),Scientific
R10252 T11585 T11559 punct .,seeded
R10253 T11586 T11587 compound Protein,concentrations
R10254 T11587 T11589 nsubjpass concentrations,determined
R10255 T11588 T11589 auxpass were,determined
R10256 T11589 T11589 ROOT determined,determined
R10257 T11590 T11589 agent by,determined
R10258 T11591 T11592 compound Bradford,assay
R10259 T11592 T11590 pobj assay,by
R10260 T11593 T11595 punct (,Scientific
R10261 T11594 T11595 compound Thermo,Scientific
R10262 T11595 T11592 appos Scientific,assay
R10263 T11596 T11595 punct ),Scientific
R10264 T11597 T11589 punct .,determined
R10265 T11598 T11600 nsubjpass Lysates,resolved
R10266 T11599 T11600 auxpass were,resolved
R10267 T11600 T11600 ROOT resolved,resolved
R10268 T11601 T11600 agent by,resolved
R10269 T11602 T11601 pobj electrophoreses,by
R10270 T11603 T11602 prep on,electrophoreses
R10271 T11604 T11605 nummod 10,%
R10272 T11605 T11607 nmod %,gels
R10273 T11606 T11607 amod SDS-polyacrylamide,gels
R10274 T11607 T11603 pobj gels,on
R10275 T11608 T11600 cc and,resolved
R10276 T11609 T11600 conj transferred,resolved
R10277 T11610 T11609 prep onto,transferred
R10278 T11611 T11612 compound PVDF,membranes
R10279 T11612 T11610 pobj membranes,onto
R10280 T11613 T11614 punct (,Millipore
R10281 T11614 T11612 appos Millipore,membranes
R10282 T11615 T11612 punct ),membranes
R10283 T11616 T11600 punct .,resolved
R10284 T11617 T11619 nsubjpass Blots,blocked
R10285 T11618 T11619 auxpass were,blocked
R10286 T11619 T11619 ROOT blocked,blocked
R10287 T11620 T11619 prep for,blocked
R10288 T11621 T11622 nummod 1,hour
R10289 T11622 T11620 pobj hour,for
R10290 T11623 T11619 prep at,blocked
R10291 T11624 T11625 compound room,temperature
R10292 T11625 T11623 pobj temperature,at
R10293 T11626 T11619 cc and,blocked
R10294 T11627 T11619 conj probed,blocked
R10295 T11628 T11627 dobj overnight,probed
R10296 T11629 T11627 prep at,probed
R10297 T11630 T11631 nummod 4,°
R10298 T11631 T11629 pobj °,at
R10299 T11632 T11627 conj C,probed
R10300 T11633 T11632 prep for,C
R10301 T11634 T11633 pobj pro-IL-1β,for
R10302 T11635 T11637 punct (,Cruz
R10303 T11636 T11637 compound Santa,Cruz
R10304 T11637 T11634 appos Cruz,pro-IL-1β
R10305 T11638 T11637 punct ),Cruz
R10306 T11639 T11634 punct ",",pro-IL-1β
R10307 T11640 T11634 conj IκBα,pro-IL-1β
R10308 T11641 T11643 punct (,Signaling
R10309 T11642 T11643 compound Cell,Signaling
R10310 T11643 T11640 parataxis Signaling,IκBα
R10311 T11644 T11643 punct ),Signaling
R10312 T11645 T11643 punct ",",Signaling
R10313 T11646 T11640 cc or,IκBα
R10314 T11647 T11640 conj β-actin,IκBα
R10315 T11648 T11650 punct (,Signaling
R10316 T11649 T11650 compound Cell,Signaling
R10317 T11650 T11647 appos Signaling,β-actin
R10318 T11651 T11647 punct ),β-actin
R10319 T11652 T11619 punct .,blocked
R10320 T11653 T11656 nsubjpass Blots,probed
R10321 T11654 T11656 auxpass were,probed
R10322 T11655 T11656 advmod subsequently,probed
R10323 T11656 T11656 ROOT probed,probed
R10324 T11657 T11656 prep with,probed
R10325 T11658 T11660 amod fluorescently-labeled,antibodies
R10326 T11659 T11660 amod secondary,antibodies
R10327 T11660 T11657 pobj antibodies,with
R10328 T11661 T11656 punct ",",probed
R10329 T11662 T11663 compound IRDye,®
R10330 T11663 T11656 punct ®,probed
R10331 T11664 T11663 nummod 680,®
R10332 T11665 T11664 cc or,680
R10333 T11666 T11663 appos 800CW,®
R10334 T11667 T11669 punct (,Biosciences
R10335 T11668 T11669 compound LI-COR,Biosciences
R10336 T11669 T11666 appos Biosciences,800CW
R10337 T11670 T11663 punct ),®
R10338 T11671 T11656 prep for,probed
R10339 T11672 T11673 nummod 1,hour
R10340 T11673 T11671 pobj hour,for
R10341 T11674 T11656 punct .,probed
R10342 T11675 T11676 det Both,blocking
R10343 T11676 T11681 nsubjpass blocking,carried
R10344 T11677 T11676 cc and,blocking
R10345 T11678 T11676 conj probing,blocking
R10346 T11679 T11681 nsubjpass steps,carried
R10347 T11680 T11681 auxpass were,carried
R10348 T11681 T11681 ROOT carried,carried
R10349 T11682 T11681 prt out,carried
R10350 T11683 T11681 prep in,carried
R10351 T11684 T11685 amod tris-buffered,saline
R10352 T11685 T11683 pobj saline,in
R10353 T11686 T11688 punct (,Biosciences
R10354 T11687 T11688 compound G,Biosciences
R10355 T11688 T11685 appos Biosciences,saline
R10356 T11689 T11688 punct ),Biosciences
R10357 T11690 T11681 prep containing,carried
R10358 T11691 T11692 nummod 5,%
R10359 T11692 T11695 compound %,albumin
R10360 T11693 T11694 amod bovine,serum
R10361 T11694 T11695 compound serum,albumin
R10362 T11695 T11690 dobj albumin,containing
R10363 T11696 T11695 cc and,albumin
R10364 T11697 T11698 nummod 0.1,%
R10365 T11698 T11700 quantmod %,20
R10366 T11699 T11700 quantmod TWEEN,20
R10367 T11700 T11695 conj 20,albumin
R10368 T11701 T11700 punct (,20
R10369 T11702 T11700 quantmod Calbiochem,20
R10370 T11703 T11700 punct ),20
R10371 T11704 T11681 punct .,carried
R10372 T11705 T11707 nsubjpass Blots,imaged
R10373 T11706 T11707 auxpass were,imaged
R10374 T11707 T11707 ROOT imaged,imaged
R10375 T11708 T11707 prep on,imaged
R10376 T11709 T11714 det a,system
R10377 T11710 T11711 compound LI-COR,Odyssey
R10378 T11711 T11714 nmod Odyssey,system
R10379 T11712 T11714 amod infrared,system
R10380 T11713 T11714 compound imaging,system
R10381 T11714 T11708 pobj system,on
R10382 T11715 T11714 punct (,system
R10383 T11716 T11717 compound LI-COR,Biosciences
R10384 T11717 T11714 appos Biosciences,system
R10385 T11718 T11714 punct ),system
R10386 T11719 T11707 cc and,imaged
R10387 T11720 T11707 conj quantified,imaged
R10388 T11721 T11720 xcomp using,quantified
R10389 T11722 T11725 det the,software
R10390 T11723 T11725 amod included,software
R10391 T11724 T11725 compound analysis,software
R10392 T11725 T11721 dobj software,using
R10393 T11726 T11707 punct .,imaged
R10563 T11929 T11937 nsubjpass Quantification,plated
R10564 T11930 T11929 prep of,Quantification
R10565 T11931 T11932 amod caspase-1,activity
R10566 T11932 T11930 pobj activity,of
R10567 T11933 T11935 amod Bronchial,cells
R10568 T11934 T11935 amod epithelial,cells
R10569 T11935 T11937 nsubjpass cells,plated
R10570 T11936 T11937 auxpass were,plated
R10571 T11937 T11937 ROOT plated,plated
R10572 T11938 T11937 prep in,plated
R10573 T11939 T11940 amod 6-well,plates
R10574 T11940 T11938 pobj plates,in
R10575 T11941 T11937 prep at,plated
R10576 T11942 T11946 nummod 5,overnight
R10577 T11943 T11946 nummod ×,overnight
R10578 T11944 T11945 nummod 105,cells/well
R10579 T11945 T11946 compound cells/well,overnight
R10580 T11946 T11941 pobj overnight,at
R10581 T11947 T11937 punct .,plated
R10582 T11948 T11950 nsubjpass Cells,primed
R10583 T11949 T11950 auxpass were,primed
R10584 T11950 T11950 ROOT primed,primed
R10585 T11951 T11950 prep with,primed
R10586 T11952 T11951 pobj LPS,with
R10587 T11953 T11950 prep for,primed
R10588 T11954 T11955 nummod 5,hours
R10589 T11955 T11953 pobj hours,for
R10590 T11956 T11950 cc and,primed
R10591 T11957 T11950 conj stimulated,primed
R10592 T11958 T11957 prep with,stimulated
R10593 T11959 T11958 pobj ATP,with
R10594 T11960 T11957 prep for,stimulated
R10595 T11961 T11962 nummod 1,hour
R10596 T11962 T11960 pobj hour,for
R10597 T11963 T11957 cc or,stimulated
R10598 T11964 T11957 conj stimulated,stimulated
R10599 T11965 T11964 prep with,stimulated
R10600 T11966 T11967 amod live,PAO1
R10601 T11967 T11965 pobj PAO1,with
R10602 T11968 T11964 prep for,stimulated
R10603 T11969 T11970 nummod 3,hours
R10604 T11970 T11968 pobj hours,for
R10605 T11971 T11950 punct .,primed
R10606 T11972 T11974 nsubjpass PBMCs,stimulated
R10607 T11973 T11974 auxpass were,stimulated
R10608 T11974 T11974 ROOT stimulated,stimulated
R10609 T11975 T11977 det the,day
R10610 T11976 T11977 amod same,day
R10611 T11977 T11974 npadvmod day,stimulated
R10612 T11978 T11977 prep as,day
R10613 T11979 T11980 compound blood,donation
R10614 T11980 T11978 pobj donation,as
R10615 T11981 T11974 punct .,stimulated
R10616 T11982 T11984 nsubjpass PBMCs,seeded
R10617 T11983 T11984 auxpass were,seeded
R10618 T11984 T11984 ROOT seeded,seeded
R10619 T11985 T11984 prep in,seeded
R10620 T11986 T11988 det a,plate
R10621 T11987 T11988 amod 96-well,plate
R10622 T11988 T11985 pobj plate,in
R10623 T11989 T11984 prep at,seeded
R10624 T11990 T11991 det a,density
R10625 T11991 T11989 pobj density,at
R10626 T11992 T11991 prep of,density
R10627 T11993 T11996 nummod 4.5,cells/well
R10628 T11994 T11996 nmod ×,cells/well
R10629 T11995 T11996 nummod 105,cells/well
R10630 T11996 T11992 pobj cells/well,of
R10631 T11997 T11996 punct (,cells/well
R10632 T11998 T12001 nummod 2.5,cells/ml
R10633 T11999 T12001 nummod ×,cells/ml
R10634 T12000 T12001 nummod 106,cells/ml
R10635 T12001 T11996 appos cells/ml,cells/well
R10636 T12002 T11996 punct ),cells/well
R10637 T12003 T11996 punct ",",cells/well
R10638 T12004 T11991 acl primed,density
R10639 T12005 T12004 prep with,primed
R10640 T12006 T12005 pobj LPS,with
R10641 T12007 T12004 prep for,primed
R10642 T12008 T12009 nummod 5,hours
R10643 T12009 T12007 pobj hours,for
R10644 T12010 T11984 cc and,seeded
R10645 T12011 T12012 advmod then,stimulated
R10646 T12012 T11984 conj stimulated,seeded
R10647 T12013 T12012 prep with,stimulated
R10648 T12014 T12013 pobj ATP,with
R10649 T12015 T12012 prep for,stimulated
R10650 T12016 T12017 nummod 1,hour
R10651 T12017 T12015 pobj hour,for
R10652 T12018 T12017 cc or,hour
R10653 T12019 T12017 conj Poly,hour
R10654 T12020 T12019 punct (,Poly
R10655 T12021 T12019 nmod dA,Poly
R10656 T12022 T12019 punct :,Poly
R10657 T12023 T12019 appos dT,Poly
R10658 T12024 T12019 punct ),Poly
R10659 T12025 T12012 prep for,stimulated
R10660 T12026 T12027 nummod 3,hours
R10661 T12027 T12025 pobj hours,for
R10662 T12028 T12012 cc or,stimulated
R10663 T12029 T12012 conj stimulated,stimulated
R10664 T12030 T12029 prep with,stimulated
R10665 T12031 T12032 amod live,PAO1
R10666 T12032 T12030 pobj PAO1,with
R10667 T12033 T12029 prep for,stimulated
R10668 T12034 T12035 nummod 3,hours
R10669 T12035 T12033 pobj hours,for
R10670 T12036 T11984 punct .,seeded
R10671 T12037 T12038 compound Caspase-1,activity
R10672 T12038 T12040 nsubjpass activity,measured
R10673 T12039 T12040 auxpass was,measured
R10674 T12040 T12040 ROOT measured,measured
R10675 T12041 T12040 advcl using,measured
R10676 T12042 T12041 dobj FLICA,using
R10677 T12043 T12045 punct (,Technologies
R10678 T12044 T12045 compound Immunochemistry,Technologies
R10679 T12045 T12042 appos Technologies,FLICA
R10680 T12046 T12045 punct ),Technologies
R10681 T12047 T12045 punct ",",Technologies
R10682 T12048 T12051 det a,probe
R10683 T12049 T12051 amod cell-permeable,probe
R10684 T12050 T12051 amod fluorescent,probe
R10685 T12051 T12045 appos probe,Technologies
R10686 T12052 T12051 punct (,probe
R10687 T12053 T12051 amod FAM-YVAD-fmk,probe
R10688 T12054 T12045 punct ),Technologies
R10689 T12055 T12056 nsubj that,binds
R10690 T12056 T12042 relcl binds,FLICA
R10691 T12057 T12058 amod active,caspase-1
R10692 T12058 T12056 dobj caspase-1,binds
R10693 T12059 T12040 punct .,measured
R10694 T12060 T12062 nsubjpass Cells,incubated
R10695 T12061 T12062 auxpass were,incubated
R10696 T12062 T12062 ROOT incubated,incubated
R10697 T12063 T12064 nummod 1,hour
R10698 T12064 T12065 npadvmod hour,with
R10699 T12065 T12062 prep with,incubated
R10700 T12066 T12065 pobj FLICA,with
R10701 T12067 T12062 prep at,incubated
R10702 T12068 T12069 nummod 37,°
R10703 T12069 T12070 nummod °,C
R10704 T12070 T12067 pobj C,at
R10705 T12071 T12062 cc and,incubated
R10706 T12072 T12062 conj stained,incubated
R10707 T12073 T12072 prep with,stained
R10708 T12074 T12076 amod PE-Cy7-conjugated,antibodies
R10709 T12075 T12076 amod anti-CD14,antibodies
R10710 T12076 T12073 pobj antibodies,with
R10711 T12077 T12078 punct (,eBioscience
R10712 T12078 T12076 appos eBioscience,antibodies
R10713 T12079 T12076 punct ),antibodies
R10714 T12080 T12081 aux to,identify
R10715 T12081 T12072 advcl identify,stained
R10716 T12082 T12081 dobj monocytes,identify
R10717 T12083 T12062 punct .,incubated
R10718 T12084 T12086 det The,strategy
R10719 T12085 T12086 amod gating,strategy
R10720 T12086 T12087 nsubj strategy,consisted
R10721 T12087 T12087 ROOT consisted,consisted
R10722 T12088 T12087 prep of,consisted
R10723 T12089 T12088 pcomp including,of
R10724 T12090 T12091 amod live,cells
R10725 T12091 T12089 pobj cells,including
R10726 T12092 T12093 nsubj that,were
R10727 T12093 T12091 relcl were,cells
R10728 T12094 T12093 attr CD14,were
R10729 T12095 T12094 amod positive,CD14
R10730 T12096 T12099 nsubjpass which,analyzed
R10731 T12097 T12099 auxpass were,analyzed
R10732 T12098 T12099 advmod subsequently,analyzed
R10733 T12099 T12095 relcl analyzed,positive
R10734 T12100 T12099 prep for,analyzed
R10735 T12101 T12102 det the,frequency
R10736 T12102 T12100 pobj frequency,for
R10737 T12103 T12102 prep of,frequency
R10738 T12104 T12106 amod FLICA,cells
R10739 T12105 T12106 amod positive,cells
R10740 T12106 T12103 pobj cells,of
R10741 T12107 T12087 punct .,consisted
R10809 T12209 T12214 compound NF-κB,Supernatants
R10810 T12210 T12214 compound /,Supernatants
R10811 T12211 T12214 compound AP-1,Supernatants
R10812 T12212 T12214 compound Activity,Supernatants
R10813 T12213 T12214 compound Assay,Supernatants
R10814 T12214 T12220 nsubjpass Supernatants,incubated
R10815 T12215 T12214 prep from,Supernatants
R10816 T12216 T12218 nummod THP-1,cells
R10817 T12217 T12218 compound reporter,cells
R10818 T12218 T12215 pobj cells,from
R10819 T12219 T12220 auxpass were,incubated
R10820 T12220 T12220 ROOT incubated,incubated
R10821 T12221 T12220 prep with,incubated
R10822 T12222 T12223 amod Quanti-Blue,substrate
R10823 T12223 T12221 pobj substrate,with
R10824 T12224 T12225 punct (,Invivogen
R10825 T12225 T12223 appos Invivogen,substrate
R10826 T12226 T12225 punct ),Invivogen
R10827 T12227 T12220 prep at,incubated
R10828 T12228 T12229 nummod 37,°
R10829 T12229 T12230 nummod °,C
R10830 T12230 T12227 pobj C,at
R10831 T12231 T12220 cc and,incubated
R10832 T12232 T12220 conj allowed,incubated
R10833 T12233 T12234 aux to,develop
R10834 T12234 T12232 xcomp develop,allowed
R10835 T12235 T12234 prep for,develop
R10836 T12236 T12235 pobj 16,for
R10837 T12237 T12238 nummod 18,hours
R10838 T12238 T12236 appos hours,16
R10839 T12239 T12220 punct .,incubated
R10840 T12240 T12241 nsubj Quanti-Blue,contains
R10841 T12241 T12241 ROOT contains,contains
R10842 T12242 T12243 det a,substrate
R10843 T12243 T12241 dobj substrate,contains
R10844 T12244 T12243 prep for,substrate
R10845 T12245 T12246 amod alkaline,phosphatase
R10846 T12246 T12244 pobj phosphatase,for
R10847 T12247 T12246 cc and,phosphatase
R10848 T12248 T12258 nsubjpass changes,quantified
R10849 T12249 T12248 prep in,changes
R10850 T12250 T12251 det the,amount
R10851 T12251 T12249 pobj amount,in
R10852 T12252 T12251 prep of,amount
R10853 T12253 T12255 compound NF-κB,AP-1
R10854 T12254 T12255 compound /,AP-1
R10855 T12255 T12256 compound AP-1,activity
R10856 T12256 T12252 pobj activity,of
R10857 T12257 T12258 auxpass were,quantified
R10858 T12258 T12243 relcl quantified,substrate
R10859 T12259 T12258 agent by,quantified
R10860 T12260 T12261 amod optical,density
R10861 T12261 T12259 pobj density,by
R10862 T12262 T12265 punct (,655
R10863 T12263 T12265 nmod λ,655
R10864 T12264 T12265 punct =,655
R10865 T12265 T12261 appos 655,density
R10866 T12266 T12261 punct ),density
R10867 T12267 T12243 acl measured,substrate
R10868 T12268 T12267 advcl using,measured
R10869 T12269 T12274 det a,reader
R10870 T12270 T12272 nmod SpectraMax,Plus
R10871 T12271 T12272 nummod 384,Plus
R10872 T12272 T12274 nmod Plus,reader
R10873 T12273 T12274 compound plate,reader
R10874 T12274 T12268 dobj reader,using
R10875 T12275 T12274 cc and,reader
R10876 T12276 T12278 nmod SoftMax,software
R10877 T12277 T12278 amod Pro,software
R10878 T12278 T12274 conj software,reader
R10879 T12279 T12281 punct (,Devices
R10880 T12280 T12281 compound Molecular,Devices
R10881 T12281 T12278 appos Devices,software
R10882 T12282 T12241 punct ),contains
R10883 T12283 T12241 punct .,contains
R11027 T12443 T12444 amod Bacterial,strains
R11028 T12444 T12455 nsubjpass strains,obtained
R11029 T12445 T12455 npadvmod P.,obtained
R11030 T12446 T12448 nmod aeruginosa,strains
R11031 T12447 T12448 compound laboratory,strains
R11032 T12448 T12455 nsubjpass strains,obtained
R11033 T12449 T12448 appos PAO1,strains
R11034 T12450 T12449 cc and,PAO1
R11035 T12451 T12453 det the,mutant
R11036 T12452 T12453 compound PAO1ΔexsA,mutant
R11037 T12453 T12449 conj mutant,PAO1
R11038 T12454 T12455 auxpass were,obtained
R11039 T12455 T12455 ROOT obtained,obtained
R11040 T12456 T12455 prep from,obtained
R11041 T12457 T12459 compound Dr.,Hancock
R11042 T12458 T12459 compound Robert,Hancock
R11043 T12459 T12456 pobj Hancock,from
R11044 T12460 T12455 punct .,obtained
R11045 T12461 T12468 nsubjpass P.,grown
R11046 T12462 T12463 compound aeruginosa,strains
R11047 T12463 T12468 nsubjpass strains,grown
R11048 T12464 T12463 appos PAO1,strains
R11049 T12465 T12464 cc and,PAO1
R11050 T12466 T12464 conj PAO1ΔexsA,PAO1
R11051 T12467 T12468 auxpass were,grown
R11052 T12468 T12468 ROOT grown,grown
R11053 T12469 T12468 prep from,grown
R11054 T12470 T12471 amod overnight,cultures
R11055 T12471 T12469 pobj cultures,from
R11056 T12472 T12471 prep in,cultures
R11057 T12473 T12474 compound Luria,Bertani
R11058 T12474 T12472 pobj Bertani,in
R11059 T12475 T12474 punct (,Bertani
R11060 T12476 T12474 appos LB,Bertani
R11061 T12477 T12474 punct ),Bertani
R11062 T12478 T12471 conj broth,cultures
R11063 T12479 T12478 cc and,broth
R11064 T12480 T12482 compound LB,streptomycin
R11065 T12481 T12482 compound +,streptomycin
R11066 T12482 T12471 appos streptomycin,cultures
R11067 T12483 T12487 punct (,ml
R11068 T12484 T12485 nummod 150,µg
R11069 T12485 T12487 nmod µg,ml
R11070 T12486 T12487 compound /,ml
R11071 T12487 T12482 appos ml,streptomycin
R11072 T12488 T12487 punct ),ml
R11073 T12489 T12469 prep until,from
R11074 T12490 T12491 amod mid-logarithmic,phase
R11075 T12491 T12489 pobj phase,until
R11076 T12492 T12468 punct .,grown
R11077 T12493 T12495 nsubjpass Cells,washed
R11078 T12494 T12495 auxpass were,washed
R11079 T12495 T12495 ROOT washed,washed
R11080 T12496 T12495 advmod once,washed
R11081 T12497 T12495 prep in,washed
R11082 T12498 T12497 pobj PBS,in
R11083 T12499 T12495 cc and,washed
R11084 T12500 T12495 conj resuspended,washed
R11085 T12501 T12500 prep in,resuspended
R11086 T12502 T12501 pobj PBS,in
R11087 T12503 T12500 prep to,resuspended
R11088 T12504 T12506 det an,density
R11089 T12505 T12506 amod optical,density
R11090 T12506 T12503 pobj density,to
R11091 T12507 T12506 prep of,density
R11092 T12508 T12507 pobj 0.5,of
R11093 T12509 T12513 punct (,nm
R11094 T12510 T12512 dep λ,600
R11095 T12511 T12512 punct =,600
R11096 T12512 T12513 nummod 600,nm
R11097 T12513 T12506 appos nm,density
R11098 T12514 T12500 punct ),resuspended
R11099 T12515 T12495 punct .,washed
R11100 T12516 T12517 aux To,prepare
R11101 T12517 T12521 advcl prepare,live
R11102 T12518 T12519 amod heat-killed,bacteria
R11103 T12519 T12517 dobj bacteria,prepare
R11104 T12520 T12521 punct ",",live
R11105 T12521 T12521 ROOT live,live
R11106 T12522 T12524 nsubjpass PAO1,resuspended
R11107 T12523 T12524 auxpass was,resuspended
R11108 T12524 T12521 ccomp resuspended,live
R11109 T12525 T12524 prep in,resuspended
R11110 T12526 T12525 pobj PBS,in
R11111 T12527 T12524 prep to,resuspended
R11112 T12528 T12530 det an,density
R11113 T12529 T12530 amod optical,density
R11114 T12530 T12527 pobj density,to
R11115 T12531 T12530 prep of,density
R11116 T12532 T12531 pobj 0.5,of
R11117 T12533 T12524 cc and,resuspended
R11118 T12534 T12524 conj heated,resuspended
R11119 T12535 T12534 prep at,heated
R11120 T12536 T12537 nummod 60,°
R11121 T12537 T12535 pobj °,at
R11122 T12538 T12524 conj C,resuspended
R11123 T12539 T12538 prep for,C
R11124 T12540 T12541 nummod 1,hour
R11125 T12541 T12539 pobj hour,for
R11126 T12542 T12521 punct .,live
R11127 T12543 T12546 prep For,live
R11128 T12544 T12543 pobj stimulations,For
R11129 T12545 T12546 punct ",",live
R11130 T12546 T12546 ROOT live,live
R11131 T12547 T12549 nsubjpass PAO1,resuspended
R11132 T12548 T12549 auxpass was,resuspended
R11133 T12549 T12546 ccomp resuspended,live
R11134 T12550 T12549 prep to,resuspended
R11135 T12551 T12553 det an,density
R11136 T12552 T12553 amod optical,density
R11137 T12553 T12550 pobj density,to
R11138 T12554 T12553 prep of,density
R11139 T12555 T12554 pobj 0.5,of
R11140 T12556 T12553 prep in,density
R11141 T12557 T12556 pobj PBS,in
R11142 T12558 T12549 cc and,resuspended
R11143 T12559 T12560 advmod further,diluted
R11144 T12560 T12549 conj diluted,resuspended
R11145 T12561 T12560 prep in,diluted
R11146 T12562 T12563 compound culture,medium
R11147 T12563 T12561 pobj medium,in
R11148 T12564 T12560 advmod prior,diluted
R11149 T12565 T12564 prep to,prior
R11150 T12566 T12565 pobj stimulation,to
R11151 T12567 T12568 aux to,achieve
R11152 T12568 T12560 advcl achieve,diluted
R11153 T12569 T12571 det the,multiplicity
R11154 T12570 T12571 amod desired,multiplicity
R11155 T12571 T12568 dobj multiplicity,achieve
R11156 T12572 T12571 prep of,multiplicity
R11157 T12573 T12572 pobj infection,of
R11158 T12574 T12546 punct .,live
R11159 T12575 T12576 compound Heat-killed,PAO1
R11160 T12576 T12578 nsubjpass PAO1,added
R11161 T12577 T12578 auxpass was,added
R11162 T12578 T12578 ROOT added,added
R11163 T12579 T12578 prep in,added
R11164 T12580 T12582 det a,equivalent
R11165 T12581 T12582 compound volume,equivalent
R11166 T12582 T12579 pobj equivalent,in
R11167 T12583 T12582 prep to,equivalent
R11168 T12584 T12585 det that,used
R11169 T12585 T12583 pobj used,to
R11170 T12586 T12587 aux to,achieve
R11171 T12587 T12585 xcomp achieve,used
R11172 T12588 T12589 det an,MOI
R11173 T12589 T12587 dobj MOI,achieve
R11174 T12590 T12589 prep of,MOI
R11175 T12591 T12590 pobj 1,of
R11176 T12592 T12587 prep for,achieve
R11177 T12593 T12594 amod live,PAO1
R11178 T12594 T12592 pobj PAO1,for
R11179 T12595 T12578 punct .,added
R11213 T12642 T12645 nsubj Statistics,display
R11214 T12643 T12644 det All,graphs
R11215 T12644 T12645 nsubj graphs,display
R11216 T12645 T12645 ROOT display,display
R11217 T12646 T12649 det the,SEM
R11218 T12647 T12649 amod mean,SEM
R11219 T12648 T12649 compound ±,SEM
R11220 T12649 T12645 dobj SEM,display
R11221 T12650 T12645 cc and,display
R11222 T12651 T12652 auxpass were,generated
R11223 T12652 T12645 conj generated,display
R11224 T12653 T12652 prep with,generated
R11225 T12654 T12653 pobj Prism,with
R11226 T12655 T12654 nummod 5,Prism
R11227 T12656 T12657 punct (,Graphpad
R11228 T12657 T12654 appos Graphpad,Prism
R11229 T12658 T12654 punct ),Prism
R11230 T12659 T12645 punct .,display
R11231 T12660 T12661 compound Statistical,significance
R11232 T12661 T12663 nsubjpass significance,determined
R11233 T12662 T12663 auxpass was,determined
R11234 T12663 T12663 ROOT determined,determined
R11235 T12664 T12663 prep by,determined
R11236 T12665 T12664 pcomp performing,by
R11237 T12666 T12665 dobj one,performing
R11238 T12667 T12666 cc or,one
R11239 T12668 T12669 amod two-way,ANOVA
R11240 T12669 T12666 conj ANOVA,one
R11241 T12670 T12669 cc and,ANOVA
R11242 T12671 T12672 det the,Bonferroni
R11243 T12672 T12669 conj Bonferroni,ANOVA
R11244 T12673 T12675 advmod post-test,applicable
R11245 T12674 T12675 advmod where,applicable
R11246 T12675 T12672 relcl applicable,Bonferroni
R11247 T12676 T12663 punct .,determined

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T9313 23-29 NNP denotes Ethics
T9314 30-39 NNP denotes Statement
T9315 40-45 NN denotes Blood
T9316 46-53 NN denotes samples
T9317 54-58 VB denotes were
T9318 59-67 VB denotes obtained
T9319 68-72 IN denotes with
T9320 73-81 VB denotes informed
T9321 82-89 VB denotes written
T9322 90-97 NN denotes consent
T9323 98-102 IN denotes from
T9324 103-110 NN denotes control
T9325 111-119 NN denotes subjects
T9326 120-123 CC denotes and
T9327 124-126 NN denotes CF
T9328 127-135 NN denotes patients
T9329 136-138 IN denotes at
T9330 139-142 DT denotes the
T9331 143-145 NNP denotes BC
T9332 146-154 NNP denotes Children
T9333 154-156 POS denotes 's
T9334 157-166 NNP denotes Hospital.
T9335 167-174 NNP denotes Consent
T9336 175-178 VB denotes was
T9337 179-187 VB denotes obtained
T9338 188-191 IN denotes for
T9339 192-200 NN denotes children
T9340 201-203 IN denotes by
T9341 204-209 PRP-DOLLAR- denotes their
T9342 210-216 NN denotes parent
T9343 217-219 CC denotes or
T9344 220-225 JJ denotes legal
T9345 226-234 NN denotes guardian
T9346 236-244 NN denotes Subjects
T9347 245-246 CD denotes 7
T9348 247-252 NN denotes years
T9349 253-255 IN denotes of
T9350 256-259 NN denotes age
T9351 260-263 CC denotes and
T9352 264-269 JJ denotes older
T9353 270-274 VB denotes were
T9354 275-283 VB denotes required
T9355 284-286 TO denotes to
T9356 287-294 VB denotes provide
T9357 295-303 JJ denotes informed
T9358 304-310 NN denotes assent
T9359 311-313 RB denotes as
T9360 314-318 RB denotes well
T9361 320-329 NN denotes Protocols
T9362 330-334 VB denotes were
T9363 335-343 VB denotes approved
T9364 344-346 IN denotes by
T9365 347-350 DT denotes the
T9366 351-359 NNP denotes Clinical
T9367 360-368 NNP denotes Research
T9368 369-375 NNP denotes Ethics
T9369 376-381 NNP denotes Board
T9370 382-383 -LRB- denotes (
T9371 383-392 NN denotes H09-01192
T9372 392-393 -RRB- denotes )
T9469 396-400 NNP denotes Cell
T9470 401-408 NNP denotes Culture
T9471 409-411 NN denotes CF
T9472 412-413 -LRB- denotes (
T9473 413-418 NN denotes IB3-1
T9474 419-422 CC denotes and
T9475 423-429 NN denotes CuFi-1
T9476 429-430 -RRB- denotes )
T9477 431-434 CC denotes and
T9478 435-442 NN denotes control
T9479 443-444 -LRB- denotes (
T9480 444-446 NN denotes S9
T9481 447-450 CC denotes and
T9482 451-457 NN denotes NuLi-1
T9483 457-458 -RRB- denotes )
T9484 459-464 NN denotes cells
T9485 465-469 VB denotes were
T9486 470-478 VB denotes obtained
T9487 479-483 IN denotes from
T9488 484-487 DT denotes the
T9489 488-496 NNP denotes American
T9490 497-501 NNP denotes Type
T9491 502-509 NNP denotes Culture
T9492 510-521 NNP denotes Collection.
T9493 522-527 NN denotes IB3-1
T9494 528-533 NN denotes cells
T9495 534-538 VB denotes were
T9496 539-546 VB denotes derived
T9497 547-551 IN denotes from
T9498 552-553 DT denotes a
T9499 554-561 NN denotes patient
T9500 562-572 VB denotes expressing
T9501 573-576 DT denotes the
T9502 577-582 NN denotes ΔF508
T9503 583-586 CC denotes and
T9504 587-593 NN denotes W1282X
T9505 594-603 NN denotes mutations
T9506 604-607 CC denotes and
T9507 608-614 NN denotes CuFi-1
T9508 615-619 VB denotes were
T9509 620-627 VB denotes derived
T9510 628-632 IN denotes from
T9511 633-634 DT denotes a
T9512 635-640 NN denotes ΔF508
T9513 641-651 JJ denotes homozygous
T9514 652-659 NN denotes patient
T9515 661-663 NN denotes S9
T9516 664-669 NN denotes cells
T9517 670-673 VB denotes are
T9518 674-679 NN denotes IB3-1
T9519 680-685 NN denotes cells
T9520 686-690 WDT denotes that
T9521 691-695 VB denotes have
T9522 696-700 VB denotes been
T9523 701-712 VB denotes transfected
T9524 713-717 IN denotes with
T9525 718-722 NN denotes CFTR
T9526 723-728 VB denotes using
T9527 729-731 DT denotes an
T9528 732-748 JJ denotes adeno-associated
T9529 749-754 JJ denotes viral
T9530 755-761 NN denotes vector
T9531 762-765 CC denotes and
T9532 766-772 NN denotes NuLi-1
T9533 773-778 NN denotes cells
T9534 779-783 VB denotes were
T9535 784-791 VB denotes derived
T9536 792-796 IN denotes from
T9537 797-798 DT denotes a
T9538 799-806 NN denotes patient
T9539 807-817 VB denotes possessing
T9540 818-819 DT denotes a
T9541 820-829 JJ denotes wild-type
T9542 830-834 NN denotes CFTR
T9543 835-843 NN denotes genotype
T9544 845-855 JJ denotes THP1-XBlue
T9545 856-861 NN denotes cells
T9546 862-868 RB denotes stably
T9547 869-876 VB denotes express
T9548 877-878 DT denotes a
T9549 879-887 VB denotes secreted
T9550 888-897 JJ denotes embryonic
T9551 898-906 NN denotes alkaline
T9552 907-918 NN denotes phosphatase
T9553 919-920 -LRB- denotes (
T9554 920-924 NN denotes SEAP
T9555 924-925 -RRB- denotes )
T9556 926-934 NN denotes reporter
T9557 935-944 JJ denotes inducible
T9558 945-947 IN denotes by
T9559 948-953 NN denotes NF-κB
T9560 954-957 CC denotes and
T9561 958-962 NN denotes AP-1
T9562 963-964 -LRB- denotes (
T9563 964-973 NN denotes Invivogen
T9564 973-974 -RRB- denotes )
T9565 976-981 NN denotes Cells
T9566 982-986 VB denotes were
T9567 987-995 VB denotes cultured
T9568 996-998 IN denotes as
T9569 999-1010 VB denotes recommended
T9570 1011-1013 IN denotes by
T9571 1014-1019 PRP-DOLLAR- denotes their
T9572 1020-1030 JJ denotes respective
T9573 1031-1040 NN denotes suppliers
T9574 1041-1046 VB denotes using
T9575 1047-1055 JJ denotes standard
T9576 1056-1065 NN denotes protocols
T9577 1067-1069 NN denotes S9
T9578 1070-1073 CC denotes and
T9579 1074-1079 NN denotes IB3-1
T9580 1080-1085 NN denotes cells
T9581 1086-1090 VB denotes were
T9582 1091-1099 VB denotes cultured
T9583 1100-1102 IN denotes in
T9584 1103-1108 JJ denotes basal
T9585 1109-1114 NN denotes LHC-8
T9586 1115-1116 -LRB- denotes (
T9587 1116-1126 NN denotes Invitrogen
T9588 1126-1127 -RRB- denotes )
T9589 1128-1140 VB denotes supplemented
T9590 1141-1145 IN denotes with
T9591 1146-1148 CD denotes 10
T9592 1148-1149 NN denotes %
T9593 1150-1151 -LRB- denotes (
T9594 1151-1154 NN denotes v/v
T9595 1154-1155 -RRB- denotes )
T9596 1156-1161 JJ denotes fetal
T9597 1162-1168 JJ denotes bovine
T9598 1169-1174 NN denotes serum
T9599 1175-1176 -LRB- denotes (
T9600 1176-1179 NN denotes FBS
T9601 1179-1180 -RRB- denotes )
T9602 1180-1181 -COMMA- denotes ,
T9603 1182-1183 CD denotes 2
T9604 1184-1186 NN denotes mM
T9605 1187-1198 NN denotes L-glutamine
T9606 1198-1199 -COMMA- denotes ,
T9607 1200-1203 CC denotes and
T9608 1204-1205 CD denotes 1
T9609 1206-1208 NN denotes mM
T9610 1209-1215 NN denotes sodium
T9611 1216-1224 NN denotes pyruvate
T9612 1226-1239 NN denotes NuLi-1/CuFi-1
T9613 1240-1245 NN denotes cells
T9614 1246-1250 VB denotes were
T9615 1251-1259 VB denotes cultured
T9616 1260-1262 IN denotes in
T9617 1263-1267 NN denotes BEBM
T9618 1268-1278 JJ denotes serum-free
T9619 1279-1285 NN denotes medium
T9620 1286-1287 -LRB- denotes (
T9621 1287-1292 NN denotes Lonza
T9622 1292-1293 -RRB- denotes )
T9623 1294-1298 IN denotes with
T9624 1299-1309 NN denotes supplement
T9625 1310-1316 NN denotes bullet
T9626 1317-1320 NN denotes kit
T9627 1321-1322 -LRB- denotes (
T9628 1322-1325 NN denotes EGF
T9629 1325-1326 -COMMA- denotes ,
T9630 1327-1341 NN denotes hydrocortisone
T9631 1341-1342 -COMMA- denotes ,
T9632 1343-1349 JJ denotes bovine
T9633 1350-1359 JJ denotes pituitary
T9634 1360-1367 NN denotes extract
T9635 1367-1368 -COMMA- denotes ,
T9636 1369-1380 NN denotes transferrin
T9637 1380-1381 -COMMA- denotes ,
T9638 1382-1388 JJ denotes bovine
T9639 1389-1396 NN denotes insulin
T9640 1396-1397 -COMMA- denotes ,
T9641 1398-1414 NN denotes triiodothyronine
T9642 1414-1415 -COMMA- denotes ,
T9643 1416-1427 NN denotes epinephrine
T9644 1427-1428 -COMMA- denotes ,
T9645 1429-1437 JJ denotes retinoic
T9646 1438-1442 NN denotes acid
T9647 1442-1443 -RRB- denotes )
T9648 1443-1444 -COMMA- denotes ,
T9649 1445-1446 CD denotes 2
T9650 1447-1449 NN denotes mM
T9651 1450-1461 NN denotes L-glutamine
T9652 1461-1462 -COMMA- denotes ,
T9653 1463-1466 CC denotes and
T9654 1467-1468 CD denotes 1
T9655 1469-1471 NN denotes mM
T9656 1472-1478 NN denotes sodium
T9657 1479-1487 NN denotes pyruvate
T9658 1489-1494 NN denotes PBMCs
T9659 1495-1499 IN denotes from
T9660 1500-1502 NN denotes CF
T9661 1503-1511 NN denotes patients
T9662 1512-1515 CC denotes and
T9663 1516-1524 NN denotes controls
T9664 1525-1529 VB denotes were
T9665 1530-1538 VB denotes cultured
T9666 1539-1541 IN denotes in
T9667 1542-1551 NN denotes RPMI-1640
T9668 1552-1553 -LRB- denotes (
T9669 1553-1560 NN denotes Hyclone
T9670 1560-1561 -RRB- denotes )
T9671 1562-1574 VB denotes supplemented
T9672 1575-1579 IN denotes with
T9673 1580-1582 CD denotes 10
T9674 1582-1583 NN denotes %
T9675 1584-1587 NNP denotes FBS
T9676 1587-1588 -COMMA- denotes ,
T9677 1589-1590 CD denotes 2
T9678 1591-1593 NN denotes mM
T9679 1594-1605 NN denotes L-glutamine
T9680 1605-1606 -COMMA- denotes ,
T9681 1607-1610 CC denotes and
T9682 1611-1612 CD denotes 1
T9683 1613-1615 NN denotes mM
T9684 1616-1622 NN denotes sodium
T9685 1623-1631 NN denotes pyruvate
T9686 1632-1633 -LRB- denotes (
T9687 1633-1641 JJ denotes complete
T9688 1642-1646 NN denotes RPMI
T9689 1646-1647 -RRB- denotes )
T9690 1649-1659 JJ denotes THP1-XBlue
T9691 1660-1665 NN denotes cells
T9692 1666-1670 VB denotes were
T9693 1671-1679 VB denotes cultured
T9694 1680-1682 IN denotes in
T9695 1683-1691 JJ denotes complete
T9696 1692-1696 NN denotes RPMI
T9697 1697-1701 IN denotes with
T9698 1702-1705 DT denotes the
T9699 1706-1714 NN denotes addition
T9700 1715-1717 IN denotes of
T9701 1718-1724 NN denotes zeocin
T9702 1725-1726 -LRB- denotes (
T9703 1726-1729 CD denotes 100
T9704 1730-1735 NN denotes µg/ml
T9705 1735-1736 -RRB- denotes )
T9706 1737-1739 TO denotes to
T9707 1740-1746 VB denotes select
T9708 1747-1750 IN denotes for
T9709 1751-1756 NN denotes cells
T9710 1757-1767 VB denotes expressing
T9711 1768-1771 DT denotes the
T9712 1772-1776 NN denotes SEAP
T9713 1777-1787 NN denotes NF-κB/AP-1
T9714 1788-1796 NN denotes reporter
T9715 1798-1803 RB denotes Prior
T9716 1804-1806 TO denotes to
T9717 1807-1818 NN denotes stimulation
T9718 1818-1819 -COMMA- denotes ,
T9719 1820-1829 JJ denotes bronchial
T9720 1830-1840 JJ denotes epithelial
T9721 1841-1845 NN denotes cell
T9722 1846-1851 NN denotes lines
T9723 1852-1856 VB denotes were
T9724 1857-1863 VB denotes plated
T9725 1864-1866 IN denotes in
T9726 1867-1873 VB denotes coated
T9727 1874-1875 -LRB- denotes [
T9728 1875-1877 CD denotes 62
T9729 1877-1878 -RRB- denotes ]
T9730 1879-1886 JJ denotes 96-well
T9731 1887-1893 NN denotes plates
T9732 1894-1895 -LRB- denotes (
T9733 1895-1897 NN denotes BD
T9734 1898-1909 NNP denotes Biosciences
T9735 1909-1910 -RRB- denotes )
T9736 1911-1913 IN denotes at
T9737 1914-1919 CD denotes 3×104
T9738 1920-1930 NN denotes cells/well
T9739 1931-1937 IN denotes unless
T9740 1938-1947 VB denotes indicated
T9741 1947-1948 -COMMA- denotes ,
T9742 1949-1952 CC denotes and
T9743 1953-1960 VB denotes allowed
T9744 1961-1963 TO denotes to
T9745 1964-1970 VB denotes adhere
T9746 1971-1980 RB denotes overnight
T9747 1982-1988 NN denotes Plates
T9748 1989-1992 IN denotes for
T9749 1993-1995 NN denotes S9
T9750 1996-1999 CC denotes and
T9751 2000-2005 NN denotes IB3-1
T9752 2006-2018 NN denotes stimulations
T9753 2019-2023 VB denotes were
T9754 2024-2030 VB denotes coated
T9755 2031-2033 IN denotes in
T9756 2034-2035 DT denotes a
T9757 2036-2043 NN denotes mixture
T9758 2044-2046 IN denotes of
T9759 2047-2053 JJ denotes bovine
T9760 2054-2059 NN denotes serum
T9761 2060-2067 NN denotes albumin
T9762 2068-2069 -LRB- denotes (
T9763 2069-2072 CD denotes 100
T9764 2073-2078 NN denotes µg/ml
T9765 2078-2079 -RRB- denotes )
T9766 2079-2080 -COMMA- denotes ,
T9767 2081-2092 NN denotes fibronectin
T9768 2093-2094 -LRB- denotes (
T9769 2094-2096 CD denotes 10
T9770 2097-2102 NN denotes µg/ml
T9771 2102-2103 -RRB- denotes )
T9772 2103-2104 -COMMA- denotes ,
T9773 2105-2108 CC denotes and
T9774 2109-2115 JJ denotes bovine
T9775 2116-2123 NN denotes collage
T9776 2124-2128 NN denotes type
T9777 2129-2130 CD denotes I
T9778 2131-2132 -LRB- denotes (
T9779 2132-2134 CD denotes 30
T9780 2135-2140 NN denotes µg/ml
T9781 2140-2141 -RRB- denotes )
T9782 2142-2143 -LRB- denotes (
T9783 2143-2145 NN denotes BD
T9784 2146-2157 NNP denotes Biosciences
T9785 2157-2158 -RRB- denotes )
T9786 2160-2166 NN denotes Plates
T9787 2167-2170 IN denotes for
T9788 2171-2177 NN denotes NuLi-1
T9789 2178-2181 CC denotes and
T9790 2182-2188 NN denotes CuFi-1
T9791 2189-2193 VB denotes were
T9792 2194-2200 VB denotes coated
T9793 2201-2205 IN denotes with
T9794 2206-2214 NN denotes collagen
T9795 2215-2219 NN denotes type
T9796 2220-2222 CD denotes IV
T9797 2223-2224 -LRB- denotes (
T9798 2224-2226 CD denotes 60
T9799 2227-2232 NN denotes µg/ml
T9800 2232-2233 -RRB- denotes )
T9801 2234-2235 -LRB- denotes (
T9802 2235-2240 NNP denotes Sigma
T9803 2241-2248 NNP denotes Aldrich
T9804 2248-2249 -RRB- denotes )
T9805 2251-2256 NN denotes PBMCs
T9806 2257-2261 VB denotes were
T9807 2262-2268 VB denotes plated
T9808 2269-2271 IN denotes in
T9809 2272-2279 JJ denotes 96-well
T9810 2280-2286 NN denotes plates
T9811 2287-2289 IN denotes at
T9812 2290-2291 DT denotes a
T9813 2292-2299 NN denotes density
T9814 2300-2302 IN denotes of
T9815 2303-2310 CD denotes 1.5×105
T9816 2311-2321 NN denotes cells/well
T9817 2322-2324 IN denotes in
T9818 2325-2328 CD denotes 200
T9819 2329-2331 NN denotes µl
T9820 2332-2333 -LRB- denotes (
T9821 2333-2340 CD denotes 7.5×105
T9822 2341-2349 NN denotes cells/mL
T9823 2349-2350 -RRB- denotes )
T9824 2352-2357 NN denotes THP-1
T9825 2358-2366 NN denotes reporter
T9826 2367-2372 NN denotes cells
T9827 2373-2377 VB denotes were
T9828 2378-2392 VB denotes differentiated
T9829 2393-2397 IN denotes into
T9830 2398-2399 DT denotes a
T9831 2400-2415 JJ denotes macrophage-like
T9832 2416-2425 NN denotes phenotype
T9833 2426-2431 VB denotes using
T9834 2432-2434 CD denotes 50
T9835 2435-2440 NN denotes ng/ml
T9836 2441-2443 IN denotes of
T9837 2444-2451 NN denotes phorbol
T9838 2452-2464 NN denotes 12-myristate
T9839 2465-2475 NN denotes 13-acetate
T9840 2476-2477 -LRB- denotes (
T9841 2477-2480 NN denotes PMA
T9842 2480-2481 -RRB- denotes )
T9843 2482-2483 -LRB- denotes (
T9844 2483-2488 NNP denotes Sigma
T9845 2489-2496 NNP denotes Aldrich
T9846 2496-2497 -RRB- denotes )
T9847 2498-2501 IN denotes for
T9848 2502-2504 CD denotes 24
T9849 2505-2510 NN denotes hours
T9850 2511-2513 IN denotes at
T9851 2514-2515 DT denotes a
T9852 2516-2523 NN denotes density
T9853 2524-2526 IN denotes of
T9854 2527-2532 CD denotes 1×105
T9855 2533-2543 NN denotes cells/well
T9856 2544-2546 IN denotes in
T9857 2547-2550 CD denotes 200
T9858 2551-2553 NN denotes µl
T9859 2554-2555 -LRB- denotes (
T9860 2555-2560 CD denotes 5×105
T9861 2561-2569 NN denotes cells/ml
T9862 2569-2570 -RRB- denotes )
T9863 2572-2577 NN denotes Cells
T9864 2578-2582 VB denotes were
T9865 2583-2589 VB denotes washed
T9866 2590-2594 IN denotes with
T9867 2595-2598 NNP denotes PBS
T9868 2599-2602 CC denotes and
T9869 2603-2610 VB denotes allowed
T9870 2611-2613 TO denotes to
T9871 2614-2618 VB denotes rest
T9872 2619-2620 DT denotes a
T9873 2621-2628 JJ denotes further
T9874 2629-2631 CD denotes 42
T9875 2632-2637 NN denotes hours
T9876 2638-2643 RB denotes prior
T9877 2644-2646 TO denotes to
T9878 2647-2658 NN denotes stimulation
T10424 2661-2663 NN denotes CF
T10425 2664-2667 CC denotes and
T10426 2668-2675 NN denotes control
T10427 2676-2683 NN denotes subject
T10428 2684-2689 NN denotes PBMCs
T10429 2690-2693 DT denotes The
T10430 2694-2703 NN denotes diagnosis
T10431 2704-2706 IN denotes of
T10432 2707-2709 NN denotes CF
T10433 2710-2713 VB denotes was
T10434 2714-2725 VB denotes established
T10435 2726-2728 IN denotes by
T10436 2729-2736 JJ denotes typical
T10437 2737-2745 JJ denotes clinical
T10438 2746-2754 NN denotes features
T10439 2754-2755 -COMMA- denotes ,
T10440 2756-2765 VB denotes increased
T10441 2766-2771 NN denotes sweat
T10442 2772-2780 NN denotes chloride
T10443 2781-2795 NN denotes concentrations
T10444 2796-2797 -LRB- denotes (
T10445 2797-2798 SYM denotes >
T10446 2798-2800 CD denotes 60
T10447 2801-2807 NN denotes mmol/l
T10448 2807-2808 -RRB- denotes )
T10449 2808-2809 -COMMA- denotes ,
T10450 2810-2813 CC denotes and
T10451 2814-2823 NN denotes detection
T10452 2824-2826 IN denotes of
T10453 2827-2838 JJ denotes CF-inducing
T10454 2839-2848 NN denotes mutations
T10455 2850-2853 DT denotes All
T10456 2854-2862 NN denotes patients
T10457 2863-2867 IN denotes with
T10458 2868-2870 NN denotes CF
T10459 2871-2875 VB denotes were
T10460 2876-2886 RB denotes clinically
T10461 2887-2893 JJ denotes stable
T10462 2894-2896 IN denotes at
T10463 2897-2900 DT denotes the
T10464 2901-2905 NN denotes time
T10465 2906-2908 IN denotes of
T10466 2909-2914 NN denotes blood
T10467 2915-2923 NN denotes donation
T10468 2923-2924 -COMMA- denotes ,
T10469 2925-2928 CC denotes and
T10470 2929-2931 PRP denotes we
T10471 2932-2940 VB denotes excluded
T10472 2941-2944 DT denotes any
T10473 2945-2953 NN denotes subjects
T10474 2954-2957 WP denotes who
T10475 2958-2962 VB denotes were
T10476 2963-2972 VB denotes receiving
T10477 2973-2981 JJ denotes systemic
T10478 2982-2997 NN denotes corticosteroids
T10479 2998-3001 JJ denotes due
T10480 3002-3004 TO denotes to
T10481 3005-3014 JJ denotes potential
T10482 3015-3031 JJ denotes immunomodulatory
T10483 3032-3040 NN denotes activity
T10484 3042-3049 NN denotes Control
T10485 3050-3057 NN denotes samples
T10486 3058-3062 VB denotes were
T10487 3063-3071 VB denotes provided
T10488 3072-3074 IN denotes by
T10489 3075-3082 JJ denotes healthy
T10490 3083-3088 JJ denotes adult
T10491 3089-3099 NN denotes volunteers
T10492 3101-3103 IN denotes In
T10493 3104-3114 RB denotes previously
T10494 3115-3124 VB denotes published
T10495 3125-3129 NN denotes work
T10496 3130-3132 PRP denotes we
T10497 3133-3137 VB denotes have
T10498 3138-3150 VB denotes demonstrated
T10499 3151-3155 IN denotes that
T10500 3156-3168 JJ denotes TLR-mediated
T10501 3169-3181 JJ denotes inflammatory
T10502 3182-3191 NN denotes responses
T10503 3192-3195 VB denotes are
T10504 3196-3202 JJ denotes stable
T10505 3203-3205 IN denotes in
T10506 3206-3212 NN denotes humans
T10507 3213-3217 IN denotes from
T10508 3218-3223 NN denotes birth
T10509 3224-3226 TO denotes to
T10510 3227-3229 CD denotes 60
T10511 3230-3235 NN denotes years
T10512 3236-3239 JJ denotes old
T10513 3240-3241 -LRB- denotes [
T10514 3241-3243 CD denotes 63
T10515 3243-3244 -RRB- denotes ]
T10516 3244-3245 -COMMA- denotes ,
T10517 3246-3255 RB denotes therefore
T10518 3256-3259 VB denotes did
T10519 3260-3263 RB denotes not
T10520 3264-3273 VB denotes age-match
T10521 3274-3277 DT denotes the
T10522 3278-3280 NN denotes CF
T10523 3281-3289 NN denotes patients
T10524 3290-3293 CC denotes and
T10525 3294-3301 JJ denotes control
T10526 3302-3310 NN denotes subjects
T10527 3312-3322 JJ denotes Peripheral
T10528 3323-3328 NN denotes blood
T10529 3329-3332 VB denotes was
T10530 3333-3342 VB denotes collected
T10531 3343-3345 IN denotes in
T10532 3346-3352 NN denotes sodium
T10533 3353-3360 NN denotes heparin
T10534 3361-3366 NN denotes tubes
T10535 3367-3368 -LRB- denotes (
T10536 3368-3370 NN denotes BD
T10537 3371-3382 NNP denotes Biosciences
T10538 3382-3383 -RRB- denotes )
T10539 3384-3387 CC denotes and
T10540 3388-3393 NN denotes PBMCs
T10541 3394-3398 VB denotes were
T10542 3399-3407 VB denotes isolated
T10543 3408-3413 VB denotes using
T10544 3414-3421 NN denotes density
T10545 3422-3430 NN denotes gradient
T10546 3431-3445 NN denotes centrifugation
T10547 3446-3448 IN denotes on
T10548 3449-3462 JJ denotes Ficoll-Paque™
T10549 3463-3467 NN denotes Plus
T10550 3468-3469 -LRB- denotes (
T10551 3469-3471 NNP denotes GE
T10552 3472-3482 NNP denotes Healthcare
T10553 3482-3483 -RRB- denotes )
T10554 3485-3488 DT denotes The
T10555 3489-3494 NN denotes layer
T10556 3495-3505 VB denotes containing
T10557 3506-3511 NN denotes PBMCs
T10558 3512-3515 VB denotes was
T10559 3516-3524 VB denotes isolated
T10560 3524-3525 -COMMA- denotes ,
T10561 3526-3532 VB denotes washed
T10562 3533-3538 RB denotes twice
T10563 3539-3541 IN denotes in
T10564 3542-3545 NNP denotes PBS
T10565 3546-3549 CC denotes and
T10566 3550-3561 VB denotes resuspended
T10567 3562-3564 IN denotes in
T10568 3565-3573 JJ denotes complete
T10569 3574-3578 NN denotes RPMI
T10570 3580-3585 NN denotes Cells
T10571 3586-3590 VB denotes were
T10572 3591-3601 VB denotes enumerated
T10573 3602-3604 IN denotes by
T10574 3605-3611 NN denotes trypan
T10575 3612-3616 JJ denotes blue
T10576 3617-3626 NN denotes exclusion
T10577 3627-3632 VB denotes using
T10578 3633-3636 DT denotes the
T10579 3637-3645 NNP denotes Countess
T10580 3646-3655 VB denotes automated
T10581 3656-3660 NN denotes cell
T10582 3661-3668 NN denotes counter
T10583 3669-3670 -LRB- denotes (
T10584 3670-3680 NN denotes Invitrogen
T10585 3680-3681 -RRB- denotes )
T10586 3683-3686 IN denotes For
T10587 3687-3697 NN denotes derivation
T10588 3698-3700 IN denotes of
T10589 3701-3712 NN denotes macrophages
T10590 3713-3717 IN denotes from
T10591 3718-3727 NN denotes monocytes
T10592 3727-3728 -COMMA- denotes ,
T10593 3729-3738 NN denotes monocytes
T10594 3739-3743 VB denotes were
T10595 3744-3751 VB denotes allowed
T10596 3752-3754 TO denotes to
T10597 3755-3761 VB denotes adhere
T10598 3762-3764 TO denotes to
T10599 3765-3772 NN denotes plastic
T10600 3773-3776 IN denotes for
T10601 3777-3778 CD denotes 2
T10602 3779-3784 NN denotes hours
T10603 3785-3787 IN denotes in
T10604 3788-3792 NN denotes RPMI
T10605 3793-3797 CD denotes 1640
T10606 3798-3803 IN denotes after
T10607 3804-3809 WDT denotes which
T10608 3810-3822 JJ denotes non-adherent
T10609 3823-3828 NN denotes cells
T10610 3829-3833 VB denotes were
T10611 3834-3841 VB denotes removed
T10612 3843-3852 NN denotes Monocytes
T10613 3853-3857 VB denotes were
T10614 3858-3865 VB denotes allowed
T10615 3866-3868 TO denotes to
T10616 3869-3882 VB denotes differentiate
T10617 3883-3885 IN denotes in
T10618 3886-3890 NN denotes RPMI
T10619 3891-3895 CD denotes 1640
T10620 3896-3908 VB denotes supplemented
T10621 3909-3913 IN denotes with
T10622 3914-3916 CD denotes 10
T10623 3916-3917 NN denotes %
T10624 3918-3923 JJ denotes human
T10625 3924-3926 NN denotes AB
T10626 3927-3932 NN denotes serum
T10627 3933-3936 IN denotes for
T10628 3937-3939 CD denotes 10
T10629 3940-3944 NN denotes days
T10872 3947-3951 NN denotes Cell
T10873 3952-3963 NN denotes stimulation
T10874 3964-3967 CC denotes and
T10875 3968-3976 NN denotes cytokine
T10876 3977-3991 NN denotes quantification
T10877 3992-4001 JJ denotes Bronchial
T10878 4002-4012 JJ denotes epithelial
T10879 4013-4018 NN denotes cells
T10880 4019-4023 VB denotes were
T10881 4024-4030 VB denotes plated
T10882 4031-4034 CC denotes and
T10883 4035-4042 VB denotes allowed
T10884 4043-4045 TO denotes to
T10885 4046-4052 VB denotes adhere
T10886 4053-4062 JJ denotes overnight
T10887 4063-4068 JJ denotes prior
T10888 4069-4071 TO denotes to
T10889 4072-4083 NN denotes stimulation
T10890 4085-4094 JJ denotes Bronchial
T10891 4095-4105 JJ denotes epithelial
T10892 4106-4111 NN denotes cells
T10893 4112-4116 VB denotes were
T10894 4117-4123 VB denotes rested
T10895 4124-4126 CC denotes or
T10896 4127-4133 VB denotes primed
T10897 4134-4138 IN denotes with
T10898 4139-4142 NN denotes LPS
T10899 4143-4146 IN denotes for
T10900 4147-4148 CD denotes 5
T10901 4149-4154 NN denotes hours
T10902 4155-4158 CC denotes and
T10903 4159-4169 VB denotes stimulated
T10904 4170-4174 IN denotes with
T10905 4175-4179 JJ denotes live
T10906 4180-4182 NNP denotes P.
T10907 4183-4193 NN denotes aeruginosa
T10908 4194-4198 NN denotes PAO1
T10909 4199-4201 CC denotes or
T10910 4202-4205 NN denotes ATP
T10911 4206-4209 IN denotes for
T10912 4210-4213 DT denotes the
T10913 4214-4219 NN denotes times
T10914 4220-4229 VB denotes indicated
T10915 4231-4236 NN denotes PBMCs
T10916 4237-4240 CC denotes and
T10917 4241-4246 NN denotes THP-1
T10918 4247-4255 NN denotes reporter
T10919 4256-4261 NN denotes cells
T10920 4262-4266 VB denotes were
T10921 4267-4273 CC denotes either
T10922 4274-4280 VB denotes rested
T10923 4281-4283 CC denotes or
T10924 4284-4290 VB denotes primed
T10925 4291-4295 IN denotes with
T10926 4296-4299 NN denotes LPS
T10927 4300-4301 -LRB- denotes (
T10928 4301-4310 NN denotes Invivogen
T10929 4310-4311 -RRB- denotes )
T10930 4312-4314 CC denotes or
T10931 4315-4326 JJ denotes heat-killed
T10932 4327-4331 NN denotes PAO1
T10933 4332-4341 NN denotes overnight
T10934 4342-4343 -LRB- denotes (
T10935 4343-4345 CD denotes 16
T10936 4346-4351 NN denotes hours
T10937 4351-4352 -RRB- denotes )
T10938 4354-4357 DT denotes The
T10939 4358-4362 JJ denotes next
T10940 4363-4366 NN denotes day
T10941 4367-4370 DT denotes the
T10942 4371-4376 NN denotes cells
T10943 4377-4381 VB denotes were
T10944 4382-4392 VB denotes challenged
T10945 4393-4397 IN denotes with
T10946 4398-4402 JJ denotes live
T10947 4403-4407 NN denotes PAO1
T10948 4407-4408 -COMMA- denotes ,
T10949 4409-4418 NN denotes PAO1ΔexsA
T10950 4418-4419 -COMMA- denotes ,
T10951 4420-4423 NN denotes ATP
T10952 4424-4425 -LRB- denotes (
T10953 4425-4434 NN denotes Invivogen
T10954 4434-4435 -RRB- denotes )
T10955 4435-4436 -COMMA- denotes ,
T10956 4437-4439 CC denotes or
T10957 4440-4444 NNP denotes Poly
T10958 4444-4445 -LRB- denotes (
T10959 4445-4447 NN denotes dA
T10960 4447-4448 -COLON- denotes :
T10961 4448-4450 NN denotes dT
T10962 4450-4451 -RRB- denotes )
T10963 4452-4453 -LRB- denotes (
T10964 4453-4458 NNP denotes Sigma
T10965 4459-4466 NNP denotes Aldrich
T10966 4466-4467 -RRB- denotes )
T10967 4468-4471 IN denotes for
T10968 4472-4475 DT denotes the
T10969 4476-4481 NN denotes times
T10970 4482-4491 VB denotes indicated
T10971 4492-4493 -LRB- denotes (
T10972 4493-4496 VB denotes see
T10973 4497-4501 NNP denotes Fig.
T10974 4502-4503 CD denotes 1
T10975 4503-4504 -RRB- denotes )
T10976 4506-4509 IN denotes For
T10977 4510-4522 NN denotes stimulations
T10978 4523-4527 IN denotes with
T10979 4528-4532 NN denotes Poly
T10980 4532-4533 -LRB- denotes (
T10981 4533-4535 NN denotes dA
T10982 4535-4536 -COLON- denotes :
T10983 4536-4538 NN denotes dT
T10984 4538-4539 -RRB- denotes )
T10985 4539-4540 -COMMA- denotes ,
T10986 4541-4554 NN denotes lipofectamine
T10987 4555-4558 NN denotes LTX
T10988 4559-4562 VB denotes was
T10989 4563-4567 VB denotes used
T10990 4568-4570 IN denotes at
T10991 4571-4572 DT denotes a
T10992 4573-4576 NN denotes 1∶1
T10993 4577-4578 -LRB- denotes (
T10994 4578-4581 NN denotes w∶v
T10995 4581-4582 -RRB- denotes )
T10996 4583-4588 NN denotes ratio
T10997 4589-4591 IN denotes of
T10998 4592-4594 NN denotes µg
T10999 4595-4597 IN denotes of
T11000 4598-4601 NN denotes DNA
T11001 4602-4604 TO denotes to
T11002 4605-4607 VB denotes µl
T11003 4608-4610 IN denotes of
T11004 4611-4624 NN denotes lipofectamine
T11005 4625-4628 CC denotes and
T11006 4629-4632 VB denotes was
T11007 4633-4638 VB denotes mixed
T11008 4639-4641 CD denotes 30
T11009 4642-4649 NN denotes minutes
T11010 4650-4655 RB denotes prior
T11011 4656-4658 TO denotes to
T11012 4659-4670 NN denotes stimulation
T11013 4672-4675 DT denotes The
T11014 4676-4681 NN denotes NF-κB
T11015 4682-4691 NN denotes inhibitor
T11016 4692-4702 NN denotes Bay11-7082
T11017 4703-4704 -LRB- denotes (
T11018 4704-4713 NN denotes Invivogen
T11019 4713-4714 -RRB- denotes )
T11020 4715-4718 VB denotes was
T11021 4719-4724 VB denotes added
T11022 4725-4727 TO denotes to
T11023 4728-4736 NN denotes cultures
T11024 4737-4738 CD denotes 1
T11025 4739-4743 NN denotes hour
T11026 4744-4749 JJ denotes prior
T11027 4750-4752 TO denotes to
T11028 4753-4760 NN denotes priming
T11029 4762-4764 IN denotes If
T11030 4765-4767 DT denotes no
T11031 4768-4775 NN denotes priming
T11032 4776-4779 VB denotes was
T11033 4780-4788 VB denotes involved
T11034 4788-4789 -COMMA- denotes ,
T11035 4790-4799 NN denotes inhibitor
T11036 4800-4803 VB denotes was
T11037 4804-4809 VB denotes added
T11038 4810-4811 CD denotes 1
T11039 4812-4816 NN denotes hour
T11040 4817-4822 JJ denotes prior
T11041 4823-4825 TO denotes to
T11042 4826-4838 JJ denotes inflammasome
T11043 4839-4850 NN denotes stimulation
T11044 4852-4855 DT denotes The
T11045 4856-4860 NNP denotes CFTR
T11046 4861-4870 NN denotes inhibitor
T11047 4871-4881 NN denotes CFTRinh172
T11048 4882-4883 -LRB- denotes (
T11049 4883-4888 NNP denotes Sigma
T11050 4889-4896 NNP denotes Aldrich
T11051 4896-4897 -RRB- denotes )
T11052 4898-4901 VB denotes was
T11053 4902-4907 VB denotes added
T11054 4908-4910 TO denotes to
T11055 4911-4919 NN denotes cultures
T11056 4920-4922 CD denotes 18
T11057 4923-4928 NN denotes hours
T11058 4929-4934 RB denotes prior
T11059 4935-4937 TO denotes to
T11060 4938-4950 JJ denotes inflammasome
T11061 4951-4962 NN denotes stimulation
T11062 4964-4967 DT denotes The
T11063 4968-4977 NN denotes caspase-1
T11064 4978-4987 NN denotes inhibitor
T11065 4988-4998 NN denotes z-YVAD-fmk
T11066 4999-5000 -LRB- denotes (
T11067 5000-5009 NN denotes Biovision
T11068 5009-5010 -RRB- denotes )
T11069 5011-5014 VB denotes was
T11070 5015-5020 VB denotes added
T11071 5021-5023 TO denotes to
T11072 5024-5032 NN denotes cultures
T11073 5033-5034 CD denotes 1
T11074 5035-5039 NN denotes hour
T11075 5040-5045 JJ denotes prior
T11076 5046-5048 TO denotes to
T11077 5049-5061 JJ denotes inflammasome
T11078 5062-5073 NN denotes stimulation
T11079 5075-5087 NN denotes Supernatants
T11080 5088-5092 VB denotes were
T11081 5093-5102 VB denotes collected
T11082 5103-5106 CC denotes and
T11083 5107-5113 VB denotes stored
T11084 5114-5116 IN denotes at
T11085 5117-5123 NNP denotes −20°C.
T11086 5124-5133 NNP denotes Cytokines
T11087 5134-5142 VB denotes released
T11088 5143-5147 IN denotes into
T11089 5148-5160 NN denotes supernatants
T11090 5161-5165 IN denotes from
T11091 5166-5171 NN denotes PBMCs
T11092 5172-5182 VB denotes stimulated
T11093 5183-5187 IN denotes with
T11094 5188-5200 JJ denotes inflammasome
T11095 5201-5211 NN denotes activators
T11096 5212-5216 VB denotes were
T11097 5217-5227 VB denotes quantified
T11098 5228-5233 VB denotes using
T11099 5234-5242 JJ denotes sandwich
T11100 5243-5248 NN denotes ELISA
T11101 5249-5250 -LRB- denotes (
T11102 5250-5261 NN denotes eBioscience
T11103 5261-5262 -RRB- denotes )
T11388 5265-5279 NN denotes Immunoblotting
T11389 5280-5285 CD denotes 1×106
T11390 5286-5291 NN denotes cells
T11391 5292-5296 VB denotes were
T11392 5297-5303 VB denotes seeded
T11393 5304-5306 IN denotes in
T11394 5307-5314 JJ denotes 12-well
T11395 5315-5321 NN denotes plates
T11396 5321-5322 -COMMA- denotes ,
T11397 5323-5333 VB denotes stimulated
T11398 5334-5336 IN denotes as
T11399 5337-5346 VB denotes indicated
T11400 5346-5347 -COMMA- denotes ,
T11401 5348-5351 CC denotes and
T11402 5352-5357 VB denotes lysed
T11403 5358-5360 IN denotes in
T11404 5361-5365 NN denotes RIPA
T11405 5366-5372 NN denotes buffer
T11406 5373-5385 VB denotes supplemented
T11407 5386-5390 IN denotes with
T11408 5391-5395 NN denotes Halt
T11409 5396-5404 NN denotes protease
T11410 5405-5408 CC denotes and
T11411 5409-5420 NN denotes phosphatase
T11412 5421-5430 NN denotes inhibitor
T11413 5431-5439 NN denotes cocktail
T11414 5440-5441 -LRB- denotes (
T11415 5441-5447 NNP denotes Thermo
T11416 5448-5458 NNP denotes Scientific
T11417 5458-5459 -RRB- denotes )
T11418 5461-5468 NN denotes Protein
T11419 5469-5483 NN denotes concentrations
T11420 5484-5488 VB denotes were
T11421 5489-5499 VB denotes determined
T11422 5500-5502 IN denotes by
T11423 5503-5511 NN denotes Bradford
T11424 5512-5517 NN denotes assay
T11425 5518-5519 -LRB- denotes (
T11426 5519-5525 NNP denotes Thermo
T11427 5526-5536 NNP denotes Scientific
T11428 5536-5537 -RRB- denotes )
T11429 5539-5546 NN denotes Lysates
T11430 5547-5551 VB denotes were
T11431 5552-5560 VB denotes resolved
T11432 5561-5563 IN denotes by
T11433 5564-5579 NN denotes electrophoreses
T11434 5580-5582 IN denotes on
T11435 5583-5585 CD denotes 10
T11436 5585-5586 NN denotes %
T11437 5587-5605 NN denotes SDS-polyacrylamide
T11438 5606-5610 NN denotes gels
T11439 5611-5614 CC denotes and
T11440 5615-5626 VB denotes transferred
T11441 5627-5631 IN denotes onto
T11442 5632-5636 NN denotes PVDF
T11443 5637-5646 NN denotes membranes
T11444 5647-5648 -LRB- denotes (
T11445 5648-5657 NNP denotes Millipore
T11446 5657-5658 -RRB- denotes )
T11447 5660-5665 NN denotes Blots
T11448 5666-5670 VB denotes were
T11449 5671-5678 VB denotes blocked
T11450 5679-5682 IN denotes for
T11451 5683-5684 CD denotes 1
T11452 5685-5689 NN denotes hour
T11453 5690-5692 IN denotes at
T11454 5693-5697 NN denotes room
T11455 5698-5709 NN denotes temperature
T11456 5710-5713 CC denotes and
T11457 5714-5720 VB denotes probed
T11458 5721-5730 RB denotes overnight
T11459 5731-5733 IN denotes at
T11460 5734-5737 NN denotes 4°C
T11461 5738-5741 IN denotes for
T11462 5742-5751 NN denotes pro-IL-1β
T11463 5752-5753 -LRB- denotes (
T11464 5753-5758 NNP denotes Santa
T11465 5759-5763 NNP denotes Cruz
T11466 5763-5764 -RRB- denotes )
T11467 5764-5765 -COMMA- denotes ,
T11468 5766-5770 NN denotes IκBα
T11469 5771-5772 -LRB- denotes (
T11470 5772-5776 NN denotes Cell
T11471 5777-5786 NN denotes Signaling
T11472 5786-5787 -RRB- denotes )
T11473 5787-5788 -COMMA- denotes ,
T11474 5789-5791 CC denotes or
T11475 5792-5799 NN denotes β-actin
T11476 5800-5801 -LRB- denotes (
T11477 5801-5805 NN denotes Cell
T11478 5806-5815 NN denotes Signaling
T11479 5815-5816 -RRB- denotes )
T11480 5818-5823 NN denotes Blots
T11481 5824-5828 VB denotes were
T11482 5829-5841 RB denotes subsequently
T11483 5842-5848 VB denotes probed
T11484 5849-5853 IN denotes with
T11485 5854-5875 JJ denotes fluorescently-labeled
T11486 5876-5885 JJ denotes secondary
T11487 5886-5896 NN denotes antibodies
T11488 5896-5897 -COMMA- denotes ,
T11489 5898-5904 NN denotes IRDye®
T11490 5905-5908 CD denotes 680
T11491 5909-5911 CC denotes or
T11492 5912-5917 NN denotes 800CW
T11493 5918-5919 -LRB- denotes (
T11494 5919-5925 NNP denotes LI-COR
T11495 5926-5937 NNP denotes Biosciences
T11496 5937-5938 -RRB- denotes )
T11497 5939-5942 IN denotes for
T11498 5943-5944 CD denotes 1
T11499 5945-5949 NN denotes hour
T11500 5951-5955 CC denotes Both
T11501 5956-5964 VB denotes blocking
T11502 5965-5968 CC denotes and
T11503 5969-5976 VB denotes probing
T11504 5977-5982 NN denotes steps
T11505 5983-5987 VB denotes were
T11506 5988-5995 VB denotes carried
T11507 5996-5999 RP denotes out
T11508 6000-6002 IN denotes in
T11509 6003-6016 JJ denotes tris-buffered
T11510 6017-6023 NN denotes saline
T11511 6024-6025 -LRB- denotes (
T11512 6025-6026 NNP denotes G
T11513 6027-6038 NNP denotes Biosciences
T11514 6038-6039 -RRB- denotes )
T11515 6040-6050 VB denotes containing
T11516 6051-6052 CD denotes 5
T11517 6052-6053 NN denotes %
T11518 6054-6060 JJ denotes bovine
T11519 6061-6066 NN denotes serum
T11520 6067-6074 NN denotes albumin
T11521 6075-6078 CC denotes and
T11522 6079-6082 CD denotes 0.1
T11523 6082-6083 NN denotes %
T11524 6084-6089 NN denotes TWEEN
T11525 6090-6092 CD denotes 20
T11526 6093-6094 -LRB- denotes (
T11527 6094-6104 NN denotes Calbiochem
T11528 6104-6105 -RRB- denotes )
T11529 6107-6112 NN denotes Blots
T11530 6113-6117 VB denotes were
T11531 6118-6124 VB denotes imaged
T11532 6125-6127 IN denotes on
T11533 6128-6129 DT denotes a
T11534 6130-6136 NNP denotes LI-COR
T11535 6137-6144 NNP denotes Odyssey
T11536 6145-6153 VB denotes infrared
T11537 6154-6161 NN denotes imaging
T11538 6162-6168 NN denotes system
T11539 6169-6170 -LRB- denotes (
T11540 6170-6176 NNP denotes LI-COR
T11541 6177-6188 NNP denotes Biosciences
T11542 6188-6189 -RRB- denotes )
T11543 6190-6193 CC denotes and
T11544 6194-6204 VB denotes quantified
T11545 6205-6210 VB denotes using
T11546 6211-6214 DT denotes the
T11547 6215-6223 VB denotes included
T11548 6224-6232 NN denotes analysis
T11549 6233-6241 NN denotes software
T11763 6244-6258 NN denotes Quantification
T11764 6259-6261 IN denotes of
T11765 6262-6271 NN denotes caspase-1
T11766 6272-6280 NN denotes activity
T11767 6281-6290 JJ denotes Bronchial
T11768 6291-6301 JJ denotes epithelial
T11769 6302-6307 NN denotes cells
T11770 6308-6312 VB denotes were
T11771 6313-6319 VB denotes plated
T11772 6320-6322 IN denotes in
T11773 6323-6329 JJ denotes 6-well
T11774 6330-6336 NN denotes plates
T11775 6337-6339 IN denotes at
T11776 6340-6345 CD denotes 5×105
T11777 6346-6356 NN denotes cells/well
T11778 6357-6366 RB denotes overnight
T11779 6368-6373 NN denotes Cells
T11780 6374-6378 VB denotes were
T11781 6379-6385 VB denotes primed
T11782 6386-6390 IN denotes with
T11783 6391-6394 NN denotes LPS
T11784 6395-6398 IN denotes for
T11785 6399-6400 CD denotes 5
T11786 6401-6406 NN denotes hours
T11787 6407-6410 CC denotes and
T11788 6411-6421 VB denotes stimulated
T11789 6422-6426 IN denotes with
T11790 6427-6430 NN denotes ATP
T11791 6431-6434 IN denotes for
T11792 6435-6436 CD denotes 1
T11793 6437-6441 NN denotes hour
T11794 6442-6444 CC denotes or
T11795 6445-6455 VB denotes stimulated
T11796 6456-6460 IN denotes with
T11797 6461-6465 JJ denotes live
T11798 6466-6470 NN denotes PAO1
T11799 6471-6474 IN denotes for
T11800 6475-6476 CD denotes 3
T11801 6477-6482 NN denotes hours
T11802 6484-6489 NN denotes PBMCs
T11803 6490-6494 VB denotes were
T11804 6495-6505 VB denotes stimulated
T11805 6506-6509 DT denotes the
T11806 6510-6514 JJ denotes same
T11807 6515-6518 NN denotes day
T11808 6519-6521 IN denotes as
T11809 6522-6527 NN denotes blood
T11810 6528-6536 NN denotes donation
T11811 6538-6543 NN denotes PBMCs
T11812 6544-6548 VB denotes were
T11813 6549-6555 VB denotes seeded
T11814 6556-6558 IN denotes in
T11815 6559-6560 DT denotes a
T11816 6561-6568 JJ denotes 96-well
T11817 6569-6574 NN denotes plate
T11818 6575-6577 IN denotes at
T11819 6578-6579 DT denotes a
T11820 6580-6587 NN denotes density
T11821 6588-6590 IN denotes of
T11822 6591-6598 CD denotes 4.5×105
T11823 6599-6609 NN denotes cells/well
T11824 6610-6611 -LRB- denotes (
T11825 6611-6618 CD denotes 2.5×106
T11826 6619-6627 NN denotes cells/ml
T11827 6627-6628 -RRB- denotes )
T11828 6628-6629 -COMMA- denotes ,
T11829 6630-6636 VB denotes primed
T11830 6637-6641 IN denotes with
T11831 6642-6645 NN denotes LPS
T11832 6646-6649 IN denotes for
T11833 6650-6651 CD denotes 5
T11834 6652-6657 NN denotes hours
T11835 6658-6661 CC denotes and
T11836 6662-6666 RB denotes then
T11837 6667-6677 VB denotes stimulated
T11838 6678-6682 IN denotes with
T11839 6683-6686 NN denotes ATP
T11840 6687-6690 IN denotes for
T11841 6691-6692 CD denotes 1
T11842 6693-6697 NN denotes hour
T11843 6698-6700 CC denotes or
T11844 6701-6705 NN denotes Poly
T11845 6705-6706 -LRB- denotes (
T11846 6706-6708 NN denotes dA
T11847 6708-6709 -COLON- denotes :
T11848 6709-6711 NN denotes dT
T11849 6711-6712 -RRB- denotes )
T11850 6713-6716 IN denotes for
T11851 6717-6718 CD denotes 3
T11852 6719-6724 NN denotes hours
T11853 6725-6727 CC denotes or
T11854 6728-6738 VB denotes stimulated
T11855 6739-6743 IN denotes with
T11856 6744-6748 JJ denotes live
T11857 6749-6753 NN denotes PAO1
T11858 6754-6757 IN denotes for
T11859 6758-6759 CD denotes 3
T11860 6760-6765 NN denotes hours
T11861 6767-6776 NN denotes Caspase-1
T11862 6777-6785 NN denotes activity
T11863 6786-6789 VB denotes was
T11864 6790-6798 VB denotes measured
T11865 6799-6804 VB denotes using
T11866 6805-6810 NN denotes FLICA
T11867 6811-6812 -LRB- denotes (
T11868 6812-6827 NNP denotes Immunochemistry
T11869 6828-6840 NNP denotes Technologies
T11870 6840-6841 -RRB- denotes )
T11871 6841-6842 -COMMA- denotes ,
T11872 6843-6844 DT denotes a
T11873 6845-6859 JJ denotes cell-permeable
T11874 6860-6871 JJ denotes fluorescent
T11875 6872-6877 NN denotes probe
T11876 6878-6879 -LRB- denotes (
T11877 6879-6891 NN denotes FAM-YVAD-fmk
T11878 6891-6892 -RRB- denotes )
T11879 6893-6897 WDT denotes that
T11880 6898-6903 VB denotes binds
T11881 6904-6910 JJ denotes active
T11882 6911-6920 NN denotes caspase-1
T11883 6922-6927 NN denotes Cells
T11884 6928-6932 VB denotes were
T11885 6933-6942 VB denotes incubated
T11886 6943-6944 CD denotes 1
T11887 6945-6949 NN denotes hour
T11888 6950-6954 IN denotes with
T11889 6955-6960 NN denotes FLICA
T11890 6961-6963 IN denotes at
T11891 6964-6968 NN denotes 37°C
T11892 6969-6972 CC denotes and
T11893 6973-6980 VB denotes stained
T11894 6981-6985 IN denotes with
T11895 6986-7003 JJ denotes PE-Cy7-conjugated
T11896 7004-7013 JJ denotes anti-CD14
T11897 7014-7024 NN denotes antibodies
T11898 7025-7026 -LRB- denotes (
T11899 7026-7037 NN denotes eBioscience
T11900 7037-7038 -RRB- denotes )
T11901 7039-7041 TO denotes to
T11902 7042-7050 VB denotes identify
T11903 7051-7060 NN denotes monocytes
T11904 7062-7065 DT denotes The
T11905 7066-7072 VB denotes gating
T11906 7073-7081 NN denotes strategy
T11907 7082-7091 VB denotes consisted
T11908 7092-7094 IN denotes of
T11909 7095-7104 VB denotes including
T11910 7105-7109 JJ denotes live
T11911 7110-7115 NN denotes cells
T11912 7116-7120 WDT denotes that
T11913 7121-7125 VB denotes were
T11914 7126-7130 NN denotes CD14
T11915 7131-7139 JJ denotes positive
T11916 7140-7145 WDT denotes which
T11917 7146-7150 VB denotes were
T11918 7151-7163 RB denotes subsequently
T11919 7164-7172 VB denotes analyzed
T11920 7173-7176 IN denotes for
T11921 7177-7180 DT denotes the
T11922 7181-7190 NN denotes frequency
T11923 7191-7193 IN denotes of
T11924 7194-7199 NN denotes FLICA
T11925 7200-7208 JJ denotes positive
T11926 7209-7214 NN denotes cells
T12143 7217-7227 NN denotes NF-κB/AP-1
T12144 7228-7236 NN denotes Activity
T12145 7237-7242 NN denotes Assay
T12146 7243-7255 NN denotes Supernatants
T12147 7256-7260 IN denotes from
T12148 7261-7266 NN denotes THP-1
T12149 7267-7275 NN denotes reporter
T12150 7276-7281 NN denotes cells
T12151 7282-7286 VB denotes were
T12152 7287-7296 VB denotes incubated
T12153 7297-7301 IN denotes with
T12154 7302-7313 JJ denotes Quanti-Blue
T12155 7314-7323 NN denotes substrate
T12156 7324-7325 -LRB- denotes (
T12157 7325-7334 NN denotes Invivogen
T12158 7334-7335 -RRB- denotes )
T12159 7336-7338 IN denotes at
T12160 7339-7343 NN denotes 37°C
T12161 7344-7347 CC denotes and
T12162 7348-7355 VB denotes allowed
T12163 7356-7358 TO denotes to
T12164 7359-7366 VB denotes develop
T12165 7367-7370 IN denotes for
T12166 7371-7376 CD denotes 16–18
T12167 7377-7382 NN denotes hours
T12168 7384-7395 NN denotes Quanti-Blue
T12169 7396-7404 VB denotes contains
T12170 7405-7406 DT denotes a
T12171 7407-7416 NN denotes substrate
T12172 7417-7420 IN denotes for
T12173 7421-7429 NN denotes alkaline
T12174 7430-7441 NN denotes phosphatase
T12175 7442-7445 CC denotes and
T12176 7446-7453 NN denotes changes
T12177 7454-7456 IN denotes in
T12178 7457-7460 DT denotes the
T12179 7461-7467 NN denotes amount
T12180 7468-7470 IN denotes of
T12181 7471-7481 NN denotes NF-κB/AP-1
T12182 7482-7490 NN denotes activity
T12183 7491-7495 VB denotes were
T12184 7496-7506 VB denotes quantified
T12185 7507-7509 IN denotes by
T12186 7510-7517 JJ denotes optical
T12187 7518-7525 NN denotes density
T12188 7526-7527 -LRB- denotes (
T12189 7527-7534 CD denotes λ = 655
T12190 7534-7535 -RRB- denotes )
T12191 7536-7544 VB denotes measured
T12192 7545-7550 VB denotes using
T12193 7551-7552 DT denotes a
T12194 7553-7563 NN denotes SpectraMax
T12195 7564-7567 CD denotes 384
T12196 7568-7572 NN denotes Plus
T12197 7573-7578 NN denotes plate
T12198 7579-7585 NN denotes reader
T12199 7586-7589 CC denotes and
T12200 7590-7597 NNP denotes SoftMax
T12201 7598-7601 NN denotes Pro
T12202 7602-7610 NN denotes software
T12203 7611-7612 -LRB- denotes (
T12204 7612-7621 NNP denotes Molecular
T12205 7622-7629 NNP denotes Devices
T12206 7629-7630 -RRB- denotes )
T12383 8102-8104 IN denotes of
T12304 7633-7642 JJ denotes Bacterial
T12305 7643-7650 NN denotes strains
T12306 7651-7653 NNP denotes P.
T12307 7654-7664 NN denotes aeruginosa
T12308 7665-7675 NN denotes laboratory
T12309 7676-7683 NN denotes strains
T12310 7684-7688 NN denotes PAO1
T12311 7689-7692 CC denotes and
T12312 7693-7696 DT denotes the
T12313 7697-7706 NN denotes PAO1ΔexsA
T12314 7707-7713 NN denotes mutant
T12315 7714-7718 VB denotes were
T12316 7719-7727 VB denotes obtained
T12317 7728-7732 IN denotes from
T12318 7733-7736 NNP denotes Dr.
T12319 7737-7743 NNP denotes Robert
T12320 7744-7752 NNP denotes Hancock.
T12321 7753-7755 NNP denotes P.
T12322 7756-7766 NN denotes aeruginosa
T12323 7767-7774 NN denotes strains
T12324 7775-7779 NN denotes PAO1
T12325 7780-7783 CC denotes and
T12326 7784-7793 NN denotes PAO1ΔexsA
T12327 7794-7798 VB denotes were
T12328 7799-7804 VB denotes grown
T12329 7805-7809 IN denotes from
T12330 7810-7819 JJ denotes overnight
T12331 7820-7828 NN denotes cultures
T12332 7829-7831 IN denotes in
T12333 7832-7837 NNP denotes Luria
T12334 7838-7845 NNP denotes Bertani
T12335 7846-7847 -LRB- denotes (
T12336 7847-7849 NN denotes LB
T12337 7849-7850 -RRB- denotes )
T12338 7851-7856 NN denotes broth
T12339 7857-7860 CC denotes and
T12340 7861-7876 NN denotes LB+streptomycin
T12341 7877-7878 -LRB- denotes (
T12342 7878-7881 CD denotes 150
T12343 7882-7887 NN denotes µg/ml
T12344 7887-7888 -RRB- denotes )
T12345 7889-7894 IN denotes until
T12346 7895-7910 JJ denotes mid-logarithmic
T12347 7911-7916 NN denotes phase
T12348 7918-7923 NN denotes Cells
T12349 7924-7928 VB denotes were
T12350 7929-7935 VB denotes washed
T12351 7936-7940 RB denotes once
T12352 7941-7943 IN denotes in
T12353 7944-7947 NNP denotes PBS
T12354 7948-7951 CC denotes and
T12355 7952-7963 VB denotes resuspended
T12356 7964-7966 IN denotes in
T12357 7967-7970 NNP denotes PBS
T12358 7971-7973 TO denotes to
T12359 7974-7976 DT denotes an
T12360 7977-7984 JJ denotes optical
T12361 7985-7992 NN denotes density
T12362 7993-7995 IN denotes of
T12363 7996-7999 CD denotes 0.5
T12364 8000-8001 -LRB- denotes (
T12365 8001-8008 CD denotes λ = 600
T12366 8009-8011 NN denotes nm
T12367 8011-8012 -RRB- denotes )
T12368 8014-8016 TO denotes To
T12369 8017-8024 VB denotes prepare
T12370 8025-8036 JJ denotes heat-killed
T12371 8037-8045 NN denotes bacteria
T12372 8045-8046 -COMMA- denotes ,
T12373 8047-8051 JJ denotes live
T12374 8052-8056 NN denotes PAO1
T12375 8057-8060 VB denotes was
T12376 8061-8072 VB denotes resuspended
T12377 8073-8075 IN denotes in
T12378 8076-8079 NNP denotes PBS
T12379 8080-8082 TO denotes to
T12380 8083-8085 DT denotes an
T12381 8086-8093 JJ denotes optical
T12382 8094-8101 NN denotes density
T12384 8105-8108 CD denotes 0.5
T12385 8109-8112 CC denotes and
T12386 8113-8119 VB denotes heated
T12387 8120-8122 IN denotes at
T12388 8123-8127 NN denotes 60°C
T12389 8128-8131 IN denotes for
T12390 8132-8133 CD denotes 1
T12391 8134-8138 NN denotes hour
T12392 8140-8143 IN denotes For
T12393 8144-8156 NN denotes stimulations
T12394 8156-8157 -COMMA- denotes ,
T12395 8158-8162 JJ denotes live
T12396 8163-8167 NN denotes PAO1
T12397 8168-8171 VB denotes was
T12398 8172-8183 VB denotes resuspended
T12399 8184-8186 TO denotes to
T12400 8187-8189 DT denotes an
T12401 8190-8197 JJ denotes optical
T12402 8198-8205 NN denotes density
T12403 8206-8208 IN denotes of
T12404 8209-8212 CD denotes 0.5
T12405 8213-8215 IN denotes in
T12406 8216-8219 NNP denotes PBS
T12407 8220-8223 CC denotes and
T12408 8224-8231 RB denotes further
T12409 8232-8239 VB denotes diluted
T12410 8240-8242 IN denotes in
T12411 8243-8250 NN denotes culture
T12412 8251-8257 NN denotes medium
T12413 8258-8263 JJ denotes prior
T12414 8264-8266 TO denotes to
T12415 8267-8278 NN denotes stimulation
T12416 8279-8281 TO denotes to
T12417 8282-8289 VB denotes achieve
T12418 8290-8293 DT denotes the
T12419 8294-8301 VB denotes desired
T12420 8302-8314 NN denotes multiplicity
T12421 8315-8317 IN denotes of
T12422 8318-8327 NN denotes infection
T12423 8329-8340 JJ denotes Heat-killed
T12424 8341-8345 NN denotes PAO1
T12425 8346-8349 VB denotes was
T12426 8350-8355 VB denotes added
T12427 8356-8358 IN denotes in
T12428 8359-8360 DT denotes a
T12429 8361-8367 NN denotes volume
T12430 8368-8378 JJ denotes equivalent
T12431 8379-8381 TO denotes to
T12432 8382-8386 DT denotes that
T12433 8387-8391 VB denotes used
T12434 8392-8394 TO denotes to
T12435 8395-8402 VB denotes achieve
T12436 8403-8405 DT denotes an
T12437 8406-8409 NN denotes MOI
T12438 8410-8412 IN denotes of
T12439 8413-8414 CD denotes 1
T12440 8415-8418 IN denotes for
T12441 8419-8423 JJ denotes live
T12442 8424-8428 NN denotes PAO1
T12609 8431-8441 NN denotes Statistics
T12610 8442-8445 DT denotes All
T12611 8446-8452 NN denotes graphs
T12612 8453-8460 VB denotes display
T12613 8461-8464 DT denotes the
T12614 8465-8469 JJ denotes mean
T12615 8470-8471 NN denotes ±
T12616 8472-8475 NN denotes SEM
T12617 8476-8479 CC denotes and
T12618 8480-8484 VB denotes were
T12619 8485-8494 VB denotes generated
T12620 8495-8499 IN denotes with
T12621 8500-8505 NN denotes Prism
T12622 8506-8507 CD denotes 5
T12623 8508-8509 -LRB- denotes (
T12624 8509-8517 NN denotes Graphpad
T12625 8517-8518 -RRB- denotes )
T12626 8520-8531 JJ denotes Statistical
T12627 8532-8544 NN denotes significance
T12628 8545-8548 VB denotes was
T12629 8549-8559 VB denotes determined
T12630 8560-8562 IN denotes by
T12631 8563-8573 VB denotes performing
T12632 8574-8577 CD denotes one
T12633 8578-8580 CC denotes or
T12634 8581-8588 JJ denotes two-way
T12635 8589-8594 NN denotes ANOVA
T12636 8595-8598 CC denotes and
T12637 8599-8602 DT denotes the
T12638 8603-8613 NNP denotes Bonferroni
T12639 8614-8623 JJ denotes post-test
T12640 8624-8629 WRB denotes where
T12641 8630-8640 JJ denotes applicable
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R8132 T9318 T9317 arg2Of obtained,were
R8133 T9318 T9319 arg1Of obtained,with
R8134 T9318 T9326 arg1Of obtained,and
R8135 T9322 T9319 arg2Of consent,with
R8136 T9322 T9320 arg2Of consent,informed
R8137 T9322 T9321 arg2Of consent,written
R8138 T9322 T9323 arg1Of consent,from
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R8142 T9328 T9329 arg1Of patients,at
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R8145 T9332 T9330 arg1Of Children,the
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R8147 T9332 T9333 arg2Of Children,'s
R8148 T9335 T9329 arg2Of Consent,at
R8149 T9335 T9333 arg1Of Consent,'s
R8150 T9335 T9334 arg1Of Consent,Hospital.
R8151 T9337 T9326 arg2Of obtained,and
R8152 T9337 T9336 arg2Of obtained,was
R8153 T9337 T9338 arg1Of obtained,for
R8154 T9339 T9338 arg2Of children,for
R8155 T9339 T9340 arg1Of children,by
R8156 T9342 T9343 arg1Of parent,or
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R8158 T9343 T9341 arg1Of or,their
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R8161 T9346 T9348 arg1Of Subjects,years
R8162 T9346 T9352 arg1Of Subjects,older
R8163 T9346 T9353 arg1Of Subjects,were
R8164 T9346 T9354 arg2Of Subjects,required
R8165 T9348 T9347 arg1Of years,7
R8166 T9348 T9349 arg1Of years,of
R8167 T9348 T9351 arg1Of years,and
R8168 T9350 T9349 arg2Of age,of
R8169 T9352 T9351 arg2Of older,and
R8170 T9354 T9353 arg2Of required,were
R8171 T9354 T9355 modOf required,to
R8172 T9356 T9355 arg1Of provide,to
R8173 T9356 T9360 arg1Of provide,well
R8174 T9358 T9356 arg2Of assent,provide
R8175 T9358 T9357 arg1Of assent,informed
R8176 T9360 T9359 arg1Of well,as
R8177 T9361 T9362 arg1Of Protocols,were
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R8179 T9363 T9362 arg2Of approved,were
R8180 T9369 T9363 arg1Of Board,approved
R8181 T9369 T9364 arg2Of Board,by
R8182 T9369 T9365 arg1Of Board,the
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R8186 T9369 T9370 arg1Of Board,(
R8187 T9371 T9370 arg2Of H09-01192,(
R8188 T9372 T9370 arg3Of ),(
R8253 T9470 T9469 arg1Of Culture,Cell
R8254 T9471 T9472 arg1Of CF,(
R8255 T9471 T9477 arg1Of CF,and
R8256 T9473 T9474 arg1Of IB3-1,and
R8257 T9474 T9472 arg2Of and,(
R8258 T9475 T9474 arg2Of CuFi-1,and
R8259 T9476 T9472 arg3Of ),(
R8260 T9477 T9495 arg1Of and,were
R8261 T9477 T9496 arg2Of and,derived
R8262 T9478 T9477 arg2Of control,and
R8263 T9478 T9479 arg1Of control,(
R8264 T9480 T9481 arg1Of S9,and
R8265 T9481 T9479 arg2Of and,(
R8266 T9482 T9481 arg2Of NuLi-1,and
R8267 T9483 T9479 arg3Of ),(
R8268 T9484 T9485 arg1Of cells,were
R8269 T9484 T9486 arg2Of cells,obtained
R8270 T9486 T9485 arg2Of obtained,were
R8271 T9486 T9487 arg1Of obtained,from
R8272 T9492 T9489 arg1Of Collection.,American
R8273 T9492 T9490 arg1Of Collection.,Type
R8274 T9492 T9491 arg1Of Collection.,Culture
R8275 T9494 T9487 arg2Of cells,from
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R8277 T9494 T9492 arg1Of cells,Collection.
R8278 T9494 T9493 arg1Of cells,IB3-1
R8279 T9496 T9485 modOf derived,were
R8280 T9496 T9495 arg2Of derived,were
R8281 T9496 T9497 arg1Of derived,from
R8282 T9496 T9506 arg1Of derived,and
R8283 T9499 T9497 arg2Of patient,from
R8284 T9499 T9498 arg1Of patient,a
R8285 T9499 T9500 arg1Of patient,expressing
R8286 T9502 T9503 arg1Of ΔF508,and
R8287 T9504 T9503 arg2Of W1282X,and
R8288 T9505 T9500 arg2Of mutations,expressing
R8289 T9505 T9501 arg1Of mutations,the
R8290 T9505 T9502 arg1Of mutations,ΔF508
R8291 T9505 T9504 arg1Of mutations,W1282X
R8292 T9507 T9508 arg1Of CuFi-1,were
R8293 T9507 T9509 arg2Of CuFi-1,derived
R8294 T9509 T9506 arg2Of derived,and
R8295 T9509 T9508 arg2Of derived,were
R8296 T9509 T9510 arg1Of derived,from
R8297 T9514 T9510 arg2Of patient,from
R8298 T9514 T9511 arg1Of patient,a
R8299 T9514 T9512 arg1Of patient,ΔF508
R8300 T9514 T9513 arg1Of patient,homozygous
R8301 T9516 T9515 arg1Of cells,S9
R8302 T9516 T9517 arg1Of cells,are
R8303 T9517 T9531 arg1Of are,and
R8304 T9519 T9517 arg2Of cells,are
R8305 T9519 T9518 arg1Of cells,IB3-1
R8306 T9519 T9520 arg1Of cells,that
R8307 T9519 T9521 arg1Of cells,have
R8308 T9519 T9522 arg1Of cells,been
R8309 T9519 T9523 arg2Of cells,transfected
R8310 T9523 T9521 arg2Of transfected,have
R8311 T9523 T9522 arg2Of transfected,been
R8312 T9523 T9524 arg1Of transfected,with
R8313 T9525 T9524 arg2Of CFTR,with
R8314 T9525 T9526 arg1Of CFTR,using
R8315 T9530 T9526 arg2Of vector,using
R8316 T9530 T9527 arg1Of vector,an
R8317 T9530 T9528 arg1Of vector,adeno-associated
R8318 T9530 T9529 arg1Of vector,viral
R8319 T9533 T9532 arg1Of cells,NuLi-1
R8320 T9533 T9534 arg1Of cells,were
R8321 T9533 T9535 arg2Of cells,derived
R8322 T9535 T9531 arg2Of derived,and
R8323 T9535 T9534 arg2Of derived,were
R8324 T9535 T9536 arg1Of derived,from
R8325 T9538 T9536 arg2Of patient,from
R8326 T9538 T9537 arg1Of patient,a
R8327 T9538 T9539 arg1Of patient,possessing
R8328 T9543 T9539 arg2Of genotype,possessing
R8329 T9543 T9540 arg1Of genotype,a
R8330 T9543 T9541 arg1Of genotype,wild-type
R8331 T9543 T9542 arg1Of genotype,CFTR
R8332 T9545 T9544 arg1Of cells,THP1-XBlue
R8333 T9545 T9547 arg1Of cells,express
R8334 T9547 T9546 arg1Of express,stably
R8335 T9554 T9553 arg2Of SEAP,(
R8336 T9555 T9553 arg3Of ),(
R8337 T9556 T9547 arg2Of reporter,express
R8338 T9556 T9548 arg1Of reporter,a
R8339 T9556 T9549 arg2Of reporter,secreted
R8340 T9556 T9550 arg1Of reporter,embryonic
R8341 T9556 T9551 arg1Of reporter,alkaline
R8342 T9556 T9552 arg1Of reporter,phosphatase
R8343 T9556 T9553 arg1Of reporter,(
R8344 T9556 T9557 arg1Of reporter,inducible
R8345 T9557 T9558 arg1Of inducible,by
R8346 T9559 T9560 arg1Of NF-κB,and
R8347 T9560 T9558 arg2Of and,by
R8348 T9560 T9562 arg1Of and,(
R8349 T9561 T9560 arg2Of AP-1,and
R8350 T9563 T9562 arg2Of Invivogen,(
R8351 T9564 T9562 arg3Of ),(
R8352 T9565 T9566 arg1Of Cells,were
R8353 T9565 T9567 arg2Of Cells,cultured
R8354 T9567 T9566 arg2Of cultured,were
R8355 T9567 T9568 arg1Of cultured,as
R8356 T9567 T9574 modOf cultured,using
R8357 T9569 T9568 arg2Of recommended,as
R8358 T9573 T9569 arg1Of suppliers,recommended
R8359 T9573 T9570 arg2Of suppliers,by
R8360 T9573 T9571 arg1Of suppliers,their
R8361 T9573 T9572 arg1Of suppliers,respective
R8362 T9576 T9574 arg2Of protocols,using
R8363 T9576 T9575 arg1Of protocols,standard
R8364 T9577 T9578 arg1Of S9,and
R8365 T9579 T9578 arg2Of IB3-1,and
R8366 T9580 T9577 arg1Of cells,S9
R8367 T9580 T9579 arg1Of cells,IB3-1
R8368 T9580 T9581 arg1Of cells,were
R8369 T9580 T9582 arg2Of cells,cultured
R8370 T9582 T9581 arg2Of cultured,were
R8371 T9582 T9583 arg1Of cultured,in
R8372 T9585 T9584 arg1Of LHC-8,basal
R8373 T9585 T9586 arg1Of LHC-8,(
R8374 T9585 T9589 arg2Of LHC-8,supplemented
R8375 T9587 T9586 arg2Of Invitrogen,(
R8376 T9588 T9586 arg3Of ),(
R8377 T9589 T9590 arg1Of supplemented,with
R8378 T9592 T9590 arg2Of %,with
R8379 T9592 T9591 arg1Of %,10
R8380 T9594 T9593 arg2Of v/v,(
R8381 T9595 T9593 arg3Of ),(
R8382 T9598 T9585 arg1Of serum,LHC-8
R8383 T9598 T9593 arg1Of serum,(
R8384 T9598 T9596 arg1Of serum,fetal
R8385 T9598 T9597 arg1Of serum,bovine
R8386 T9598 T9599 arg1Of serum,(
R8387 T9598 T9602 arg1Of serum,","
R8388 T9600 T9599 arg2Of FBS,(
R8389 T9601 T9599 arg3Of ),(
R8390 T9602 T9607 arg1Of ",",and
R8391 T9603 T9604 arg1Of 2,mM
R8392 T9605 T9602 arg2Of L-glutamine,","
R8393 T9605 T9603 arg1Of L-glutamine,2
R8394 T9607 T9583 arg2Of and,in
R8395 T9607 T9606 arg1Of and,","
R8396 T9608 T9609 arg1Of 1,mM
R8397 T9611 T9607 arg2Of pyruvate,and
R8398 T9611 T9608 arg1Of pyruvate,1
R8399 T9611 T9610 arg1Of pyruvate,sodium
R8400 T9613 T9612 arg1Of cells,NuLi-1/CuFi-1
R8401 T9613 T9614 arg1Of cells,were
R8402 T9613 T9615 arg2Of cells,cultured
R8403 T9615 T9614 arg2Of cultured,were
R8404 T9615 T9616 arg1Of cultured,in
R8405 T9619 T9617 arg1Of medium,BEBM
R8406 T9619 T9618 arg1Of medium,serum-free
R8407 T9619 T9620 arg1Of medium,(
R8408 T9619 T9623 arg1Of medium,with
R8409 T9619 T9653 arg1Of medium,and
R8410 T9621 T9620 arg2Of Lonza,(
R8411 T9622 T9620 arg3Of ),(
R8412 T9626 T9624 arg1Of kit,supplement
R8413 T9626 T9625 arg1Of kit,bullet
R8414 T9626 T9627 arg1Of kit,(
R8415 T9626 T9648 arg1Of kit,","
R8416 T9628 T9629 arg1Of EGF,","
R8417 T9628 T9631 arg1Of EGF,","
R8418 T9628 T9635 arg1Of EGF,","
R8419 T9628 T9637 arg1Of EGF,","
R8420 T9630 T9629 arg2Of hydrocortisone,","
R8421 T9634 T9631 arg2Of extract,","
R8422 T9634 T9632 arg1Of extract,bovine
R8423 T9634 T9633 arg1Of extract,pituitary
R8424 T9636 T9635 arg2Of transferrin,","
R8425 T9637 T9627 arg2Of ",",(
R8426 T9637 T9640 arg1Of ",",","
R8427 T9639 T9637 arg2Of insulin,","
R8428 T9639 T9638 arg1Of insulin,bovine
R8429 T9641 T9640 arg2Of triiodothyronine,","
R8430 T9641 T9642 arg1Of triiodothyronine,","
R8431 T9641 T9644 arg1Of triiodothyronine,","
R8432 T9643 T9642 arg2Of epinephrine,","
R8433 T9646 T9644 arg2Of acid,","
R8434 T9646 T9645 arg1Of acid,retinoic
R8435 T9647 T9627 arg3Of ),(
R8436 T9648 T9623 arg2Of ",",with
R8437 T9649 T9650 arg1Of 2,mM
R8438 T9651 T9648 arg2Of L-glutamine,","
R8439 T9651 T9649 arg1Of L-glutamine,2
R8440 T9653 T9616 arg2Of and,in
R8441 T9653 T9652 arg1Of and,","
R8442 T9654 T9655 arg1Of 1,mM
R8443 T9657 T9653 arg2Of pyruvate,and
R8444 T9657 T9654 arg1Of pyruvate,1
R8445 T9657 T9656 arg1Of pyruvate,sodium
R8446 T9658 T9659 arg1Of PBMCs,from
R8447 T9658 T9664 arg1Of PBMCs,were
R8448 T9658 T9665 arg2Of PBMCs,cultured
R8449 T9661 T9660 arg1Of patients,CF
R8450 T9661 T9662 arg1Of patients,and
R8451 T9662 T9659 arg2Of and,from
R8452 T9663 T9662 arg2Of controls,and
R8453 T9665 T9664 arg2Of cultured,were
R8454 T9665 T9666 arg1Of cultured,in
R8455 T9667 T9668 arg1Of RPMI-1640,(
R8456 T9667 T9671 arg2Of RPMI-1640,supplemented
R8457 T9667 T9681 arg1Of RPMI-1640,and
R8458 T9669 T9668 arg2Of Hyclone,(
R8459 T9670 T9668 arg3Of ),(
R8460 T9671 T9672 arg1Of supplemented,with
R8461 T9674 T9673 arg1Of %,10
R8462 T9675 T9672 arg2Of FBS,with
R8463 T9675 T9674 arg1Of FBS,%
R8464 T9675 T9676 arg1Of FBS,","
R8465 T9677 T9678 arg1Of 2,mM
R8466 T9679 T9676 arg2Of L-glutamine,","
R8467 T9679 T9677 arg1Of L-glutamine,2
R8468 T9681 T9666 arg2Of and,in
R8469 T9681 T9680 arg1Of and,","
R8470 T9682 T9683 arg1Of 1,mM
R8471 T9685 T9681 arg2Of pyruvate,and
R8472 T9685 T9682 arg1Of pyruvate,1
R8473 T9685 T9684 arg1Of pyruvate,sodium
R8474 T9685 T9686 arg1Of pyruvate,(
R8475 T9688 T9686 arg2Of RPMI,(
R8476 T9688 T9687 arg1Of RPMI,complete
R8477 T9689 T9686 arg3Of ),(
R8478 T9691 T9690 arg1Of cells,THP1-XBlue
R8479 T9691 T9692 arg1Of cells,were
R8480 T9691 T9693 arg2Of cells,cultured
R8481 T9693 T9692 arg2Of cultured,were
R8482 T9693 T9694 arg1Of cultured,in
R8483 T9693 T9697 arg1Of cultured,with
R8484 T9696 T9694 arg2Of RPMI,in
R8485 T9696 T9695 arg1Of RPMI,complete
R8486 T9699 T9697 arg2Of addition,with
R8487 T9699 T9698 arg1Of addition,the
R8488 T9699 T9700 arg1Of addition,of
R8489 T9699 T9706 modOf addition,to
R8490 T9701 T9700 arg2Of zeocin,of
R8491 T9701 T9702 arg1Of zeocin,(
R8492 T9704 T9702 arg2Of µg/ml,(
R8493 T9704 T9703 arg1Of µg/ml,100
R8494 T9705 T9702 arg3Of ),(
R8495 T9707 T9706 arg1Of select,to
R8496 T9707 T9708 arg1Of select,for
R8497 T9709 T9708 arg2Of cells,for
R8498 T9709 T9710 arg1Of cells,expressing
R8499 T9714 T9710 arg2Of reporter,expressing
R8500 T9714 T9711 arg1Of reporter,the
R8501 T9714 T9712 arg1Of reporter,SEAP
R8502 T9714 T9713 arg1Of reporter,NF-κB/AP-1
R8503 T9715 T9716 arg1Of Prior,to
R8504 T9717 T9716 arg2Of stimulation,to
R8505 T9722 T9719 arg1Of lines,bronchial
R8506 T9722 T9720 arg1Of lines,epithelial
R8507 T9722 T9721 arg1Of lines,cell
R8508 T9722 T9723 arg1Of lines,were
R8509 T9722 T9724 arg2Of lines,plated
R8510 T9724 T9715 arg1Of plated,Prior
R8511 T9724 T9718 arg1Of plated,","
R8512 T9724 T9723 arg2Of plated,were
R8513 T9724 T9725 arg1Of plated,in
R8514 T9724 T9736 arg1Of plated,at
R8515 T9724 T9739 arg1Of plated,unless
R8516 T9728 T9727 arg2Of 62,[
R8517 T9729 T9727 arg3Of ],[
R8518 T9731 T9725 arg2Of plates,in
R8519 T9731 T9726 arg1Of plates,coated
R8520 T9731 T9727 arg1Of plates,[
R8521 T9731 T9730 arg1Of plates,96-well
R8522 T9731 T9732 arg1Of plates,(
R8523 T9733 T9732 arg2Of BD,(
R8524 T9733 T9734 arg1Of BD,Biosciences
R8525 T9735 T9732 arg3Of ),(
R8526 T9738 T9736 arg2Of cells/well,at
R8527 T9738 T9737 arg1Of cells/well,3×104
R8528 T9740 T9742 arg1Of indicated,and
R8529 T9742 T9739 arg2Of and,unless
R8530 T9742 T9741 arg1Of and,","
R8531 T9743 T9742 arg2Of allowed,and
R8532 T9745 T9743 arg3Of adhere,allowed
R8533 T9745 T9744 arg1Of adhere,to
R8534 T9745 T9746 arg1Of adhere,overnight
R8535 T9747 T9748 arg1Of Plates,for
R8536 T9747 T9753 arg1Of Plates,were
R8537 T9747 T9754 arg2Of Plates,coated
R8538 T9749 T9750 arg1Of S9,and
R8539 T9751 T9750 arg2Of IB3-1,and
R8540 T9752 T9748 arg2Of stimulations,for
R8541 T9752 T9749 arg1Of stimulations,S9
R8542 T9752 T9751 arg1Of stimulations,IB3-1
R8543 T9754 T9753 arg2Of coated,were
R8544 T9754 T9755 arg1Of coated,in
R8545 T9757 T9755 arg2Of mixture,in
R8546 T9757 T9756 arg1Of mixture,a
R8547 T9757 T9758 arg1Of mixture,of
R8548 T9761 T9759 arg1Of albumin,bovine
R8549 T9761 T9760 arg1Of albumin,serum
R8550 T9761 T9762 arg1Of albumin,(
R8551 T9761 T9766 arg1Of albumin,","
R8552 T9764 T9762 arg2Of µg/ml,(
R8553 T9764 T9763 arg1Of µg/ml,100
R8554 T9765 T9762 arg3Of ),(
R8555 T9766 T9773 arg1Of ",",and
R8556 T9767 T9766 arg2Of fibronectin,","
R8557 T9767 T9768 arg1Of fibronectin,(
R8558 T9770 T9768 arg2Of µg/ml,(
R8559 T9770 T9769 arg1Of µg/ml,10
R8560 T9771 T9768 arg3Of ),(
R8561 T9773 T9758 arg2Of and,of
R8562 T9773 T9772 arg1Of and,","
R8563 T9773 T9782 arg1Of and,(
R8564 T9776 T9773 arg2Of type,and
R8565 T9776 T9774 arg1Of type,bovine
R8566 T9776 T9775 arg1Of type,collage
R8567 T9776 T9777 arg1Of type,I
R8568 T9776 T9778 arg1Of type,(
R8569 T9780 T9778 arg2Of µg/ml,(
R8570 T9780 T9779 arg1Of µg/ml,30
R8571 T9781 T9778 arg3Of ),(
R8572 T9784 T9782 arg2Of Biosciences,(
R8573 T9784 T9783 arg1Of Biosciences,BD
R8574 T9785 T9782 arg3Of ),(
R8575 T9786 T9787 arg1Of Plates,for
R8576 T9786 T9791 arg1Of Plates,were
R8577 T9786 T9792 arg2Of Plates,coated
R8578 T9788 T9789 arg1Of NuLi-1,and
R8579 T9789 T9787 arg2Of and,for
R8580 T9790 T9789 arg2Of CuFi-1,and
R8581 T9792 T9791 arg2Of coated,were
R8582 T9792 T9793 arg1Of coated,with
R8583 T9795 T9793 arg2Of type,with
R8584 T9795 T9794 arg1Of type,collagen
R8585 T9795 T9796 arg1Of type,IV
R8586 T9795 T9797 arg1Of type,(
R8587 T9795 T9801 arg1Of type,(
R8588 T9799 T9797 arg2Of µg/ml,(
R8589 T9799 T9798 arg1Of µg/ml,60
R8590 T9800 T9797 arg3Of ),(
R8591 T9803 T9801 arg2Of Aldrich,(
R8592 T9803 T9802 arg1Of Aldrich,Sigma
R8593 T9804 T9801 arg3Of ),(
R8594 T9805 T9806 arg1Of PBMCs,were
R8595 T9805 T9807 arg2Of PBMCs,plated
R8596 T9807 T9806 arg2Of plated,were
R8597 T9807 T9808 arg1Of plated,in
R8598 T9807 T9811 arg1Of plated,at
R8599 T9810 T9808 arg2Of plates,in
R8600 T9810 T9809 arg1Of plates,96-well
R8601 T9813 T9811 arg2Of density,at
R8602 T9813 T9812 arg1Of density,a
R8603 T9813 T9814 arg1Of density,of
R8604 T9813 T9817 arg1Of density,in
R8605 T9816 T9814 arg2Of cells/well,of
R8606 T9816 T9815 arg1Of cells/well,1.5×105
R8607 T9819 T9817 arg2Of µl,in
R8608 T9819 T9818 arg1Of µl,200
R8609 T9819 T9820 arg1Of µl,(
R8610 T9822 T9820 arg2Of cells/mL,(
R8611 T9822 T9821 arg1Of cells/mL,7.5×105
R8612 T9823 T9820 arg3Of ),(
R8613 T9826 T9824 arg1Of cells,THP-1
R8614 T9826 T9825 arg1Of cells,reporter
R8615 T9826 T9827 arg1Of cells,were
R8616 T9826 T9828 arg2Of cells,differentiated
R8617 T9828 T9827 arg2Of differentiated,were
R8618 T9828 T9829 arg1Of differentiated,into
R8619 T9832 T9829 arg2Of phenotype,into
R8620 T9832 T9830 arg1Of phenotype,a
R8621 T9832 T9831 arg1Of phenotype,macrophage-like
R8622 T9832 T9833 arg1Of phenotype,using
R8623 T9833 T9847 arg1Of using,for
R8624 T9835 T9833 arg2Of ng/ml,using
R8625 T9835 T9834 arg1Of ng/ml,50
R8626 T9835 T9836 arg1Of ng/ml,of
R8627 T9839 T9836 arg2Of 13-acetate,of
R8628 T9839 T9837 arg1Of 13-acetate,phorbol
R8629 T9839 T9838 arg1Of 13-acetate,12-myristate
R8630 T9839 T9840 arg1Of 13-acetate,(
R8631 T9839 T9843 arg1Of 13-acetate,(
R8632 T9841 T9840 arg2Of PMA,(
R8633 T9842 T9840 arg3Of ),(
R8634 T9845 T9843 arg2Of Aldrich,(
R8635 T9845 T9844 arg1Of Aldrich,Sigma
R8636 T9846 T9843 arg3Of ),(
R8637 T9849 T9847 arg2Of hours,for
R8638 T9849 T9848 arg1Of hours,24
R8639 T9849 T9850 arg1Of hours,at
R8640 T9852 T9850 arg2Of density,at
R8641 T9852 T9851 arg1Of density,a
R8642 T9852 T9853 arg1Of density,of
R8643 T9852 T9856 arg1Of density,in
R8644 T9855 T9853 arg2Of cells/well,of
R8645 T9855 T9854 arg1Of cells/well,1×105
R8646 T9858 T9856 arg2Of µl,in
R8647 T9858 T9857 arg1Of µl,200
R8648 T9858 T9859 arg1Of µl,(
R8649 T9861 T9859 arg2Of cells/ml,(
R8650 T9861 T9860 arg1Of cells/ml,5×105
R8651 T9862 T9859 arg3Of ),(
R8652 T9863 T9864 arg1Of Cells,were
R8653 T9863 T9865 arg2Of Cells,washed
R8654 T9863 T9869 arg2Of Cells,allowed
R8655 T9863 T9871 arg1Of Cells,rest
R8656 T9865 T9866 arg1Of washed,with
R8657 T9865 T9868 arg1Of washed,and
R8658 T9867 T9866 arg2Of PBS,with
R8659 T9868 T9864 arg2Of and,were
R8660 T9869 T9868 arg2Of allowed,and
R8661 T9871 T9869 arg3Of rest,allowed
R8662 T9871 T9870 arg1Of rest,to
R8663 T9871 T9876 arg1Of rest,prior
R8664 T9875 T9871 arg2Of hours,rest
R8665 T9875 T9872 arg1Of hours,a
R8666 T9875 T9873 arg1Of hours,further
R8667 T9875 T9874 arg1Of hours,42
R8668 T9876 T9877 arg1Of prior,to
R8669 T9878 T9877 arg2Of stimulation,to
R9132 T10424 T10425 arg1Of CF,and
R9133 T10426 T10425 arg2Of control,and
R9134 T10428 T10424 arg1Of PBMCs,CF
R9135 T10428 T10426 arg1Of PBMCs,control
R9136 T10428 T10427 arg1Of PBMCs,subject
R9137 T10430 T10429 arg1Of diagnosis,The
R9138 T10430 T10431 arg1Of diagnosis,of
R9139 T10430 T10433 arg1Of diagnosis,was
R9140 T10430 T10434 arg2Of diagnosis,established
R9141 T10432 T10431 arg2Of CF,of
R9142 T10434 T10433 arg2Of established,was
R9143 T10438 T10436 arg1Of features,typical
R9144 T10438 T10437 arg1Of features,clinical
R9145 T10438 T10439 arg1Of features,","
R9146 T10439 T10450 arg1Of ",",and
R9147 T10443 T10439 arg2Of concentrations,","
R9148 T10443 T10440 arg2Of concentrations,increased
R9149 T10443 T10441 arg1Of concentrations,sweat
R9150 T10443 T10442 arg1Of concentrations,chloride
R9151 T10443 T10444 arg1Of concentrations,(
R9152 T10446 T10445 arg1Of 60,>
R9153 T10447 T10444 arg2Of mmol/l,(
R9154 T10447 T10446 arg1Of mmol/l,60
R9155 T10448 T10444 arg3Of ),(
R9156 T10450 T10434 arg1Of and,established
R9157 T10450 T10435 arg2Of and,by
R9158 T10450 T10449 arg1Of and,","
R9159 T10451 T10450 arg2Of detection,and
R9160 T10451 T10452 arg1Of detection,of
R9161 T10454 T10452 arg2Of mutations,of
R9162 T10454 T10453 arg1Of mutations,CF-inducing
R9163 T10456 T10455 arg1Of patients,All
R9164 T10456 T10457 arg1Of patients,with
R9165 T10456 T10459 arg1Of patients,were
R9166 T10456 T10461 arg1Of patients,stable
R9167 T10458 T10457 arg2Of CF,with
R9168 T10459 T10469 arg1Of were,and
R9169 T10461 T10459 arg2Of stable,were
R9170 T10461 T10460 arg1Of stable,clinically
R9171 T10461 T10462 arg1Of stable,at
R9172 T10464 T10462 arg2Of time,at
R9173 T10464 T10463 arg1Of time,the
R9174 T10464 T10465 arg1Of time,of
R9175 T10467 T10465 arg2Of donation,of
R9176 T10467 T10466 arg1Of donation,blood
R9177 T10469 T10468 arg1Of and,","
R9178 T10470 T10471 arg1Of we,excluded
R9179 T10471 T10469 arg2Of excluded,and
R9180 T10473 T10471 arg2Of subjects,excluded
R9181 T10473 T10472 arg1Of subjects,any
R9182 T10473 T10474 arg1Of subjects,who
R9183 T10473 T10475 arg1Of subjects,were
R9184 T10473 T10476 arg1Of subjects,receiving
R9185 T10476 T10475 arg2Of receiving,were
R9186 T10478 T10476 arg2Of corticosteroids,receiving
R9187 T10478 T10477 arg1Of corticosteroids,systemic
R9188 T10478 T10479 arg1Of corticosteroids,due
R9189 T10479 T10480 arg1Of due,to
R9190 T10483 T10480 arg2Of activity,to
R9191 T10483 T10481 arg1Of activity,potential
R9192 T10483 T10482 arg1Of activity,immunomodulatory
R9193 T10485 T10484 arg1Of samples,Control
R9194 T10485 T10486 arg1Of samples,were
R9195 T10485 T10487 arg2Of samples,provided
R9196 T10487 T10486 arg2Of provided,were
R9197 T10490 T10489 arg1Of adult,healthy
R9198 T10491 T10487 arg1Of volunteers,provided
R9199 T10491 T10488 arg2Of volunteers,by
R9200 T10491 T10490 arg1Of volunteers,adult
R9201 T10494 T10493 arg1Of published,previously
R9202 T10495 T10492 arg2Of work,In
R9203 T10495 T10494 arg1Of work,published
R9204 T10496 T10497 arg1Of we,have
R9205 T10496 T10498 arg1Of we,demonstrated
R9206 T10498 T10492 arg1Of demonstrated,In
R9207 T10498 T10497 arg2Of demonstrated,have
R9208 T10502 T10500 arg1Of responses,TLR-mediated
R9209 T10502 T10501 arg1Of responses,inflammatory
R9210 T10502 T10503 arg1Of responses,are
R9211 T10502 T10504 arg1Of responses,stable
R9212 T10502 T10518 arg1Of responses,did
R9213 T10502 T10520 arg1Of responses,age-match
R9214 T10503 T10516 arg1Of are,","
R9215 T10504 T10503 arg2Of stable,are
R9216 T10504 T10505 arg1Of stable,in
R9217 T10506 T10505 arg2Of humans,in
R9218 T10506 T10507 arg1Of humans,from
R9219 T10508 T10507 arg2Of birth,from
R9220 T10508 T10512 arg1Of birth,old
R9221 T10508 T10513 arg1Of birth,[
R9222 T10511 T10510 arg1Of years,60
R9223 T10512 T10509 arg1Of old,to
R9224 T10512 T10511 arg1Of old,years
R9225 T10514 T10513 arg2Of 63,[
R9226 T10515 T10513 arg3Of ],[
R9227 T10516 T10498 arg2Of ",",demonstrated
R9228 T10516 T10499 arg1Of ",",that
R9229 T10520 T10516 arg2Of age-match,","
R9230 T10520 T10517 arg1Of age-match,therefore
R9231 T10520 T10518 arg2Of age-match,did
R9232 T10520 T10519 arg1Of age-match,not
R9233 T10523 T10522 arg1Of patients,CF
R9234 T10523 T10524 arg1Of patients,and
R9235 T10524 T10520 arg2Of and,age-match
R9236 T10524 T10521 arg1Of and,the
R9237 T10526 T10524 arg2Of subjects,and
R9238 T10526 T10525 arg1Of subjects,control
R9239 T10528 T10527 arg1Of blood,Peripheral
R9240 T10528 T10529 arg1Of blood,was
R9241 T10528 T10530 arg2Of blood,collected
R9242 T10530 T10529 arg2Of collected,was
R9243 T10530 T10531 arg1Of collected,in
R9244 T10530 T10539 arg1Of collected,and
R9245 T10534 T10531 arg2Of tubes,in
R9246 T10534 T10532 arg1Of tubes,sodium
R9247 T10534 T10533 arg1Of tubes,heparin
R9248 T10534 T10535 arg1Of tubes,(
R9249 T10537 T10535 arg2Of Biosciences,(
R9250 T10537 T10536 arg1Of Biosciences,BD
R9251 T10538 T10535 arg3Of ),(
R9252 T10540 T10541 arg1Of PBMCs,were
R9253 T10540 T10542 arg2Of PBMCs,isolated
R9254 T10542 T10539 arg2Of isolated,and
R9255 T10542 T10541 arg2Of isolated,were
R9256 T10542 T10543 modOf isolated,using
R9257 T10546 T10543 arg2Of centrifugation,using
R9258 T10546 T10544 arg1Of centrifugation,density
R9259 T10546 T10545 arg1Of centrifugation,gradient
R9260 T10546 T10547 arg1Of centrifugation,on
R9261 T10549 T10547 arg2Of Plus,on
R9262 T10549 T10548 arg1Of Plus,Ficoll-Paque™
R9263 T10549 T10550 arg1Of Plus,(
R9264 T10552 T10550 arg2Of Healthcare,(
R9265 T10552 T10551 arg1Of Healthcare,GE
R9266 T10553 T10550 arg3Of ),(
R9267 T10557 T10554 arg1Of PBMCs,The
R9268 T10557 T10555 arg1Of PBMCs,layer
R9269 T10557 T10556 arg1Of PBMCs,containing
R9270 T10557 T10558 arg1Of PBMCs,was
R9271 T10557 T10559 arg2Of PBMCs,isolated
R9272 T10557 T10561 arg2Of PBMCs,washed
R9273 T10557 T10566 arg1Of PBMCs,resuspended
R9274 T10559 T10560 arg1Of isolated,","
R9275 T10560 T10565 arg1Of ",",and
R9276 T10561 T10560 arg2Of washed,","
R9277 T10561 T10562 arg1Of washed,twice
R9278 T10561 T10563 arg1Of washed,in
R9279 T10564 T10563 arg2Of PBS,in
R9280 T10565 T10558 arg2Of and,was
R9281 T10566 T10565 arg2Of resuspended,and
R9282 T10566 T10567 arg1Of resuspended,in
R9283 T10569 T10567 arg2Of RPMI,in
R9284 T10569 T10568 arg1Of RPMI,complete
R9285 T10570 T10571 arg1Of Cells,were
R9286 T10570 T10572 arg2Of Cells,enumerated
R9287 T10572 T10571 arg2Of enumerated,were
R9288 T10576 T10572 arg1Of exclusion,enumerated
R9289 T10576 T10573 arg2Of exclusion,by
R9290 T10576 T10574 arg1Of exclusion,trypan
R9291 T10576 T10575 arg1Of exclusion,blue
R9292 T10576 T10577 arg1Of exclusion,using
R9293 T10582 T10577 arg2Of counter,using
R9294 T10582 T10578 arg1Of counter,the
R9295 T10582 T10579 arg1Of counter,Countess
R9296 T10582 T10580 arg2Of counter,automated
R9297 T10582 T10581 arg1Of counter,cell
R9298 T10582 T10583 arg1Of counter,(
R9299 T10584 T10583 arg2Of Invitrogen,(
R9300 T10585 T10583 arg3Of ),(
R9301 T10587 T10586 arg2Of derivation,For
R9302 T10587 T10588 arg1Of derivation,of
R9303 T10587 T10590 arg1Of derivation,from
R9304 T10589 T10588 arg2Of macrophages,of
R9305 T10591 T10590 arg2Of monocytes,from
R9306 T10593 T10594 arg1Of monocytes,were
R9307 T10593 T10595 arg2Of monocytes,allowed
R9308 T10593 T10597 arg1Of monocytes,adhere
R9309 T10595 T10586 arg1Of allowed,For
R9310 T10595 T10592 arg1Of allowed,","
R9311 T10595 T10594 arg2Of allowed,were
R9312 T10597 T10595 arg3Of adhere,allowed
R9313 T10597 T10596 arg1Of adhere,to
R9314 T10597 T10598 arg1Of adhere,to
R9315 T10597 T10600 arg1Of adhere,for
R9316 T10599 T10598 arg2Of plastic,to
R9317 T10602 T10600 arg2Of hours,for
R9318 T10602 T10601 arg1Of hours,2
R9319 T10602 T10603 arg1Of hours,in
R9320 T10602 T10606 arg2Of hours,after
R9321 T10602 T10607 arg1Of hours,which
R9322 T10604 T10603 arg2Of RPMI,in
R9323 T10604 T10605 arg1Of RPMI,1640
R9324 T10609 T10608 arg1Of cells,non-adherent
R9325 T10609 T10610 arg1Of cells,were
R9326 T10609 T10611 arg2Of cells,removed
R9327 T10611 T10606 arg1Of removed,after
R9328 T10611 T10610 arg2Of removed,were
R9329 T10612 T10613 arg1Of Monocytes,were
R9330 T10612 T10614 arg2Of Monocytes,allowed
R9331 T10612 T10616 arg1Of Monocytes,differentiate
R9332 T10614 T10613 arg2Of allowed,were
R9333 T10616 T10614 arg3Of differentiate,allowed
R9334 T10616 T10615 arg1Of differentiate,to
R9335 T10616 T10617 arg1Of differentiate,in
R9336 T10616 T10627 arg1Of differentiate,for
R9337 T10618 T10617 arg2Of RPMI,in
R9338 T10618 T10619 arg1Of RPMI,1640
R9339 T10618 T10620 arg2Of RPMI,supplemented
R9340 T10620 T10621 arg1Of supplemented,with
R9341 T10622 T10623 arg1Of 10,%
R9342 T10626 T10621 arg2Of serum,with
R9343 T10626 T10622 arg1Of serum,10
R9344 T10626 T10624 arg1Of serum,human
R9345 T10626 T10625 arg1Of serum,AB
R9346 T10629 T10627 arg2Of days,for
R9347 T10629 T10628 arg1Of days,10
R9564 T10873 T10872 arg1Of stimulation,Cell
R9565 T10873 T10874 arg1Of stimulation,and
R9566 T10876 T10874 arg2Of quantification,and
R9567 T10876 T10875 arg1Of quantification,cytokine
R9568 T10879 T10877 arg1Of cells,Bronchial
R9569 T10879 T10878 arg1Of cells,epithelial
R9570 T10879 T10880 arg1Of cells,were
R9571 T10879 T10881 arg2Of cells,plated
R9572 T10879 T10883 arg2Of cells,allowed
R9573 T10879 T10885 arg1Of cells,adhere
R9574 T10881 T10882 arg1Of plated,and
R9575 T10882 T10880 arg2Of and,were
R9576 T10883 T10882 arg2Of allowed,and
R9577 T10885 T10883 arg3Of adhere,allowed
R9578 T10885 T10884 arg1Of adhere,to
R9579 T10886 T10888 arg1Of overnight,to
R9580 T10888 T10887 arg1Of to,prior
R9581 T10889 T10885 arg2Of stimulation,adhere
R9582 T10889 T10886 arg1Of stimulation,overnight
R9583 T10892 T10890 arg1Of cells,Bronchial
R9584 T10892 T10891 arg1Of cells,epithelial
R9585 T10892 T10893 arg1Of cells,were
R9586 T10892 T10894 arg2Of cells,rested
R9587 T10892 T10896 arg2Of cells,primed
R9588 T10892 T10903 arg2Of cells,stimulated
R9589 T10894 T10895 arg1Of rested,or
R9590 T10895 T10893 arg2Of or,were
R9591 T10895 T10914 arg2Of or,indicated
R9592 T10896 T10897 arg1Of primed,with
R9593 T10896 T10899 arg1Of primed,for
R9594 T10896 T10902 arg1Of primed,and
R9595 T10898 T10897 arg2Of LPS,with
R9596 T10901 T10899 arg2Of hours,for
R9597 T10901 T10900 arg1Of hours,5
R9598 T10902 T10895 arg2Of and,or
R9599 T10903 T10902 arg2Of stimulated,and
R9600 T10903 T10904 arg1Of stimulated,with
R9601 T10906 T10904 arg2Of P.,with
R9602 T10906 T10905 arg1Of P.,live
R9603 T10908 T10907 arg1Of PAO1,aeruginosa
R9604 T10908 T10909 arg1Of PAO1,or
R9605 T10909 T10911 arg1Of or,for
R9606 T10909 T10914 arg1Of or,indicated
R9607 T10910 T10909 arg2Of ATP,or
R9608 T10913 T10911 arg2Of times,for
R9609 T10913 T10912 arg1Of times,the
R9610 T10915 T10916 arg1Of PBMCs,and
R9611 T10916 T10920 arg1Of and,were
R9612 T10916 T10922 arg2Of and,rested
R9613 T10916 T10924 arg2Of and,primed
R9614 T10919 T10916 arg2Of cells,and
R9615 T10919 T10917 arg1Of cells,THP-1
R9616 T10919 T10918 arg1Of cells,reporter
R9617 T10922 T10923 arg1Of rested,or
R9618 T10923 T10920 arg2Of or,were
R9619 T10923 T10921 arg1Of or,either
R9620 T10924 T10923 arg2Of primed,or
R9621 T10924 T10925 arg1Of primed,with
R9622 T10926 T10927 arg1Of LPS,(
R9623 T10926 T10930 arg1Of LPS,or
R9624 T10928 T10927 arg2Of Invivogen,(
R9625 T10929 T10927 arg3Of ),(
R9626 T10930 T10925 arg2Of or,with
R9627 T10933 T10930 arg2Of overnight,or
R9628 T10933 T10931 arg1Of overnight,heat-killed
R9629 T10933 T10932 arg1Of overnight,PAO1
R9630 T10933 T10934 arg1Of overnight,(
R9631 T10936 T10934 arg2Of hours,(
R9632 T10936 T10935 arg1Of hours,16
R9633 T10937 T10934 arg3Of ),(
R9634 T10940 T10939 arg1Of day,next
R9635 T10940 T10956 arg1Of day,or
R9636 T10942 T10941 arg1Of cells,the
R9637 T10942 T10943 arg1Of cells,were
R9638 T10942 T10944 arg2Of cells,challenged
R9639 T10944 T10940 arg2Of challenged,day
R9640 T10944 T10943 arg2Of challenged,were
R9641 T10944 T10945 arg1Of challenged,with
R9642 T10947 T10945 arg2Of PAO1,with
R9643 T10947 T10946 arg1Of PAO1,live
R9644 T10947 T10948 arg1Of PAO1,","
R9645 T10947 T10950 arg1Of PAO1,","
R9646 T10949 T10948 arg2Of PAO1ΔexsA,","
R9647 T10951 T10950 arg2Of ATP,","
R9648 T10951 T10952 arg1Of ATP,(
R9649 T10953 T10952 arg2Of Invivogen,(
R9650 T10954 T10952 arg3Of ),(
R9651 T10956 T10938 arg1Of or,The
R9652 T10956 T10955 arg1Of or,","
R9653 T10956 T10970 arg1Of or,indicated
R9654 T10957 T10956 arg2Of Poly,or
R9655 T10957 T10958 arg1Of Poly,(
R9656 T10957 T10963 arg1Of Poly,(
R9657 T10957 T10967 arg1Of Poly,for
R9658 T10959 T10958 arg2Of dA,(
R9659 T10959 T10960 arg1Of dA,:
R9660 T10959 T10961 arg1Of dA,dT
R9661 T10962 T10958 arg3Of ),(
R9662 T10965 T10963 arg2Of Aldrich,(
R9663 T10965 T10964 arg1Of Aldrich,Sigma
R9664 T10966 T10963 arg3Of ),(
R9665 T10969 T10967 arg2Of times,for
R9666 T10969 T10968 arg1Of times,the
R9667 T10970 T10971 arg1Of indicated,(
R9668 T10972 T10971 arg2Of see,(
R9669 T10973 T10972 arg2Of Fig.,see
R9670 T10973 T10974 arg1Of Fig.,1
R9671 T10975 T10971 arg3Of ),(
R9672 T10977 T10976 arg2Of stimulations,For
R9673 T10977 T10978 arg1Of stimulations,with
R9674 T10979 T10978 arg2Of Poly,with
R9675 T10979 T10980 arg1Of Poly,(
R9676 T10981 T10980 arg2Of dA,(
R9677 T10981 T10982 arg1Of dA,:
R9678 T10981 T10983 arg1Of dA,dT
R9679 T10984 T10980 arg3Of ),(
R9680 T10987 T10986 arg1Of LTX,lipofectamine
R9681 T10987 T10988 arg1Of LTX,was
R9682 T10987 T10989 arg2Of LTX,used
R9683 T10987 T11006 arg1Of LTX,was
R9684 T10987 T11007 arg2Of LTX,mixed
R9685 T10989 T10988 arg2Of used,was
R9686 T10989 T10990 arg1Of used,at
R9687 T10989 T11005 arg1Of used,and
R9688 T10994 T10993 arg2Of w∶v,(
R9689 T10995 T10993 arg3Of ),(
R9690 T10996 T10990 arg2Of ratio,at
R9691 T10996 T10991 arg1Of ratio,a
R9692 T10996 T10992 arg1Of ratio,1∶1
R9693 T10996 T10993 arg1Of ratio,(
R9694 T10996 T10997 arg1Of ratio,of
R9695 T10996 T11001 modOf ratio,to
R9696 T10998 T10997 arg2Of µg,of
R9697 T10998 T10999 arg1Of µg,of
R9698 T11000 T10999 arg2Of DNA,of
R9699 T11002 T11001 arg1Of µl,to
R9700 T11002 T11003 arg1Of µl,of
R9701 T11004 T11003 arg2Of lipofectamine,of
R9702 T11005 T10976 arg1Of and,For
R9703 T11005 T10985 arg1Of and,","
R9704 T11007 T11005 arg2Of mixed,and
R9705 T11007 T11006 arg2Of mixed,was
R9706 T11007 T11010 arg1Of mixed,prior
R9707 T11009 T11008 arg1Of minutes,30
R9708 T11010 T11009 arg1Of prior,minutes
R9709 T11010 T11011 arg1Of prior,to
R9710 T11012 T11011 arg2Of stimulation,to
R9711 T11016 T11013 arg1Of Bay11-7082,The
R9712 T11016 T11014 arg1Of Bay11-7082,NF-κB
R9713 T11016 T11015 arg1Of Bay11-7082,inhibitor
R9714 T11016 T11017 arg1Of Bay11-7082,(
R9715 T11016 T11020 arg1Of Bay11-7082,was
R9716 T11016 T11021 arg2Of Bay11-7082,added
R9717 T11018 T11017 arg2Of Invivogen,(
R9718 T11019 T11017 arg3Of ),(
R9719 T11021 T11020 arg2Of added,was
R9720 T11021 T11022 arg1Of added,to
R9721 T11025 T11022 arg2Of hour,to
R9722 T11025 T11023 arg1Of hour,cultures
R9723 T11025 T11024 arg1Of hour,1
R9724 T11025 T11026 arg1Of hour,prior
R9725 T11026 T11027 arg1Of prior,to
R9726 T11028 T11027 arg2Of priming,to
R9727 T11031 T11030 arg1Of priming,no
R9728 T11031 T11032 arg1Of priming,was
R9729 T11031 T11033 arg2Of priming,involved
R9730 T11033 T11029 arg2Of involved,If
R9731 T11033 T11032 arg2Of involved,was
R9732 T11035 T11036 arg1Of inhibitor,was
R9733 T11035 T11037 arg2Of inhibitor,added
R9734 T11037 T11029 arg1Of added,If
R9735 T11037 T11034 arg1Of added,","
R9736 T11037 T11036 arg2Of added,was
R9737 T11039 T11037 arg3Of hour,added
R9738 T11039 T11038 arg1Of hour,1
R9739 T11039 T11040 arg1Of hour,prior
R9740 T11040 T11041 arg1Of prior,to
R9741 T11043 T11041 arg2Of stimulation,to
R9742 T11043 T11042 arg1Of stimulation,inflammasome
R9743 T11047 T11044 arg1Of CFTRinh172,The
R9744 T11047 T11045 arg1Of CFTRinh172,CFTR
R9745 T11047 T11046 arg1Of CFTRinh172,inhibitor
R9746 T11047 T11048 arg1Of CFTRinh172,(
R9747 T11047 T11052 arg1Of CFTRinh172,was
R9748 T11047 T11053 arg2Of CFTRinh172,added
R9749 T11050 T11048 arg2Of Aldrich,(
R9750 T11050 T11049 arg1Of Aldrich,Sigma
R9751 T11051 T11048 arg3Of ),(
R9752 T11053 T11052 arg2Of added,was
R9753 T11053 T11054 arg1Of added,to
R9754 T11053 T11058 arg1Of added,prior
R9755 T11055 T11054 arg2Of cultures,to
R9756 T11057 T11056 arg1Of hours,18
R9757 T11058 T11057 arg1Of prior,hours
R9758 T11058 T11059 arg1Of prior,to
R9759 T11061 T11059 arg2Of stimulation,to
R9760 T11061 T11060 arg1Of stimulation,inflammasome
R9761 T11065 T11062 arg1Of z-YVAD-fmk,The
R9762 T11065 T11063 arg1Of z-YVAD-fmk,caspase-1
R9763 T11065 T11064 arg1Of z-YVAD-fmk,inhibitor
R9764 T11065 T11066 arg1Of z-YVAD-fmk,(
R9765 T11065 T11069 arg1Of z-YVAD-fmk,was
R9766 T11065 T11070 arg2Of z-YVAD-fmk,added
R9767 T11067 T11066 arg2Of Biovision,(
R9768 T11068 T11066 arg3Of ),(
R9769 T11070 T11069 arg2Of added,was
R9770 T11070 T11071 arg1Of added,to
R9771 T11074 T11071 arg2Of hour,to
R9772 T11074 T11072 arg1Of hour,cultures
R9773 T11074 T11073 arg1Of hour,1
R9774 T11074 T11075 arg1Of hour,prior
R9775 T11075 T11076 arg1Of prior,to
R9776 T11078 T11076 arg2Of stimulation,to
R9777 T11078 T11077 arg1Of stimulation,inflammasome
R9778 T11079 T11080 arg1Of Supernatants,were
R9779 T11079 T11081 arg2Of Supernatants,collected
R9780 T11079 T11083 arg2Of Supernatants,stored
R9781 T11081 T11082 arg1Of collected,and
R9782 T11082 T11080 arg2Of and,were
R9783 T11083 T11082 arg2Of stored,and
R9784 T11083 T11084 arg1Of stored,at
R9785 T11086 T11085 arg1Of Cytokines,−20°C.
R9786 T11086 T11087 arg2Of Cytokines,released
R9787 T11086 T11096 arg1Of Cytokines,were
R9788 T11086 T11097 arg2Of Cytokines,quantified
R9789 T11087 T11088 arg1Of released,into
R9790 T11089 T11088 arg2Of supernatants,into
R9791 T11089 T11090 arg1Of supernatants,from
R9792 T11091 T11090 arg2Of PBMCs,from
R9793 T11091 T11092 arg2Of PBMCs,stimulated
R9794 T11092 T11093 arg1Of stimulated,with
R9795 T11095 T11093 arg2Of activators,with
R9796 T11095 T11094 arg1Of activators,inflammasome
R9797 T11097 T11080 modOf quantified,were
R9798 T11097 T11096 arg2Of quantified,were
R9799 T11098 T11097 arg3Of using,quantified
R9800 T11100 T11098 arg2Of ELISA,using
R9801 T11100 T11099 arg1Of ELISA,sandwich
R9802 T11100 T11101 arg1Of ELISA,(
R9803 T11102 T11101 arg2Of eBioscience,(
R9804 T11103 T11101 arg3Of ),(
R10054 T11390 T11389 arg1Of cells,1×106
R10055 T11390 T11391 arg1Of cells,were
R10056 T11390 T11392 arg2Of cells,seeded
R10057 T11390 T11402 arg2Of cells,lysed
R10058 T11392 T11393 arg1Of seeded,in
R10059 T11392 T11401 arg1Of seeded,and
R10060 T11395 T11393 arg2Of plates,in
R10061 T11395 T11394 arg1Of plates,12-well
R10062 T11395 T11396 arg1Of plates,","
R10063 T11395 T11397 arg2Of plates,stimulated
R10064 T11397 T11398 arg1Of stimulated,as
R10065 T11399 T11398 arg2Of indicated,as
R10066 T11401 T11391 arg2Of and,were
R10067 T11401 T11400 arg1Of and,","
R10068 T11402 T11401 arg2Of lysed,and
R10069 T11402 T11403 arg1Of lysed,in
R10070 T11405 T11403 arg2Of buffer,in
R10071 T11405 T11404 arg1Of buffer,RIPA
R10072 T11405 T11406 arg2Of buffer,supplemented
R10073 T11406 T11407 arg1Of supplemented,with
R10074 T11409 T11408 arg1Of protease,Halt
R10075 T11409 T11410 arg1Of protease,and
R10076 T11410 T11407 arg2Of and,with
R10077 T11413 T11410 arg2Of cocktail,and
R10078 T11413 T11411 arg1Of cocktail,phosphatase
R10079 T11413 T11412 arg1Of cocktail,inhibitor
R10080 T11413 T11414 arg1Of cocktail,(
R10081 T11416 T11414 arg2Of Scientific,(
R10082 T11416 T11415 arg1Of Scientific,Thermo
R10083 T11417 T11414 arg3Of ),(
R10084 T11419 T11418 arg1Of concentrations,Protein
R10085 T11419 T11420 arg1Of concentrations,were
R10086 T11419 T11421 arg2Of concentrations,determined
R10087 T11421 T11420 arg2Of determined,were
R10088 T11424 T11421 arg1Of assay,determined
R10089 T11424 T11422 arg2Of assay,by
R10090 T11424 T11423 arg1Of assay,Bradford
R10091 T11424 T11425 arg1Of assay,(
R10092 T11427 T11425 arg2Of Scientific,(
R10093 T11427 T11426 arg1Of Scientific,Thermo
R10094 T11428 T11425 arg3Of ),(
R10095 T11429 T11430 arg1Of Lysates,were
R10096 T11429 T11431 arg2Of Lysates,resolved
R10097 T11429 T11440 arg2Of Lysates,transferred
R10098 T11431 T11432 arg1Of resolved,by
R10099 T11431 T11439 arg1Of resolved,and
R10100 T11433 T11432 arg2Of electrophoreses,by
R10101 T11433 T11434 arg1Of electrophoreses,on
R10102 T11435 T11436 arg1Of 10,%
R10103 T11438 T11434 arg2Of gels,on
R10104 T11438 T11435 arg1Of gels,10
R10105 T11438 T11437 arg1Of gels,SDS-polyacrylamide
R10106 T11439 T11430 arg2Of and,were
R10107 T11440 T11439 arg2Of transferred,and
R10108 T11440 T11441 arg1Of transferred,onto
R10109 T11443 T11441 arg2Of membranes,onto
R10110 T11443 T11442 arg1Of membranes,PVDF
R10111 T11443 T11444 arg1Of membranes,(
R10112 T11445 T11444 arg2Of Millipore,(
R10113 T11446 T11444 arg3Of ),(
R10114 T11447 T11448 arg1Of Blots,were
R10115 T11447 T11449 arg2Of Blots,blocked
R10116 T11447 T11457 arg1Of Blots,probed
R10117 T11449 T11448 arg2Of blocked,were
R10118 T11449 T11450 arg1Of blocked,for
R10119 T11449 T11456 arg1Of blocked,and
R10120 T11452 T11450 arg2Of hour,for
R10121 T11452 T11451 arg1Of hour,1
R10122 T11452 T11453 arg1Of hour,at
R10123 T11455 T11453 arg2Of temperature,at
R10124 T11455 T11454 arg1Of temperature,room
R10125 T11457 T11456 arg2Of probed,and
R10126 T11457 T11458 arg1Of probed,overnight
R10127 T11457 T11459 arg1Of probed,at
R10128 T11460 T11459 arg2Of 4°C,at
R10129 T11460 T11461 arg1Of 4°C,for
R10130 T11462 T11463 arg1Of pro-IL-1β,(
R10131 T11462 T11467 arg1Of pro-IL-1β,","
R10132 T11465 T11463 arg2Of Cruz,(
R10133 T11465 T11464 arg1Of Cruz,Santa
R10134 T11466 T11463 arg3Of ),(
R10135 T11467 T11474 arg1Of ",",or
R10136 T11468 T11467 arg2Of IκBα,","
R10137 T11468 T11469 arg1Of IκBα,(
R10138 T11471 T11469 arg2Of Signaling,(
R10139 T11471 T11470 arg1Of Signaling,Cell
R10140 T11472 T11469 arg3Of ),(
R10141 T11474 T11461 arg2Of or,for
R10142 T11474 T11473 arg1Of or,","
R10143 T11475 T11474 arg2Of β-actin,or
R10144 T11475 T11476 arg1Of β-actin,(
R10145 T11478 T11476 arg2Of Signaling,(
R10146 T11478 T11477 arg1Of Signaling,Cell
R10147 T11479 T11476 arg3Of ),(
R10148 T11480 T11481 arg1Of Blots,were
R10149 T11480 T11483 arg2Of Blots,probed
R10150 T11483 T11481 arg2Of probed,were
R10151 T11483 T11482 arg1Of probed,subsequently
R10152 T11483 T11484 arg1Of probed,with
R10153 T11483 T11497 arg1Of probed,for
R10154 T11487 T11484 arg2Of antibodies,with
R10155 T11487 T11485 arg1Of antibodies,fluorescently-labeled
R10156 T11487 T11486 arg1Of antibodies,secondary
R10157 T11487 T11488 arg1Of antibodies,","
R10158 T11489 T11490 arg1Of IRDye®,680
R10159 T11489 T11491 arg1Of IRDye®,or
R10160 T11491 T11488 arg2Of or,","
R10161 T11492 T11491 arg2Of 800CW,or
R10162 T11492 T11493 arg1Of 800CW,(
R10163 T11495 T11493 arg2Of Biosciences,(
R10164 T11495 T11494 arg1Of Biosciences,LI-COR
R10165 T11496 T11493 arg3Of ),(
R10166 T11499 T11497 arg2Of hour,for
R10167 T11499 T11498 arg1Of hour,1
R10168 T11504 T11501 arg1Of steps,blocking
R10169 T11504 T11502 arg1Of steps,and
R10170 T11504 T11503 arg1Of steps,probing
R10171 T11504 T11505 arg1Of steps,were
R10172 T11504 T11506 arg2Of steps,carried
R10173 T11506 T11500 arg1Of carried,Both
R10174 T11506 T11505 arg2Of carried,were
R10175 T11506 T11507 arg1Of carried,out
R10176 T11506 T11508 arg1Of carried,in
R10177 T11510 T11508 arg2Of saline,in
R10178 T11510 T11509 arg1Of saline,tris-buffered
R10179 T11510 T11511 arg1Of saline,(
R10180 T11510 T11515 arg1Of saline,containing
R10181 T11513 T11511 arg2Of Biosciences,(
R10182 T11513 T11512 arg1Of Biosciences,G
R10183 T11514 T11511 arg3Of ),(
R10184 T11516 T11517 arg1Of 5,%
R10185 T11520 T11516 arg1Of albumin,5
R10186 T11520 T11518 arg1Of albumin,bovine
R10187 T11520 T11519 arg1Of albumin,serum
R10188 T11520 T11521 arg1Of albumin,and
R10189 T11521 T11515 arg2Of and,containing
R10190 T11522 T11523 arg1Of 0.1,%
R10191 T11524 T11521 arg2Of TWEEN,and
R10192 T11524 T11522 arg1Of TWEEN,0.1
R10193 T11524 T11525 arg1Of TWEEN,20
R10194 T11524 T11526 arg1Of TWEEN,(
R10195 T11527 T11526 arg2Of Calbiochem,(
R10196 T11528 T11526 arg3Of ),(
R10197 T11529 T11530 arg1Of Blots,were
R10198 T11529 T11531 arg2Of Blots,imaged
R10199 T11529 T11544 arg2Of Blots,quantified
R10200 T11529 T11545 arg1Of Blots,using
R10201 T11531 T11532 arg1Of imaged,on
R10202 T11531 T11543 arg1Of imaged,and
R10203 T11535 T11532 arg2Of Odyssey,on
R10204 T11535 T11533 arg1Of Odyssey,a
R10205 T11535 T11534 arg1Of Odyssey,LI-COR
R10206 T11538 T11531 arg3Of system,imaged
R10207 T11538 T11536 arg2Of system,infrared
R10208 T11538 T11537 arg1Of system,imaging
R10209 T11538 T11539 arg1Of system,(
R10210 T11541 T11539 arg2Of Biosciences,(
R10211 T11541 T11540 arg1Of Biosciences,LI-COR
R10212 T11542 T11539 arg3Of ),(
R10213 T11543 T11530 arg2Of and,were
R10214 T11544 T11543 arg2Of quantified,and
R10215 T11545 T11544 arg3Of using,quantified
R10216 T11549 T11545 arg2Of software,using
R10217 T11549 T11546 arg1Of software,the
R10218 T11549 T11547 arg2Of software,included
R10219 T11549 T11548 arg1Of software,analysis
R10394 T11763 T11764 arg1Of Quantification,of
R10395 T11766 T11764 arg2Of activity,of
R10396 T11766 T11765 arg1Of activity,caspase-1
R10397 T11769 T11767 arg1Of cells,Bronchial
R10398 T11769 T11768 arg1Of cells,epithelial
R10399 T11769 T11770 arg1Of cells,were
R10400 T11769 T11771 arg2Of cells,plated
R10401 T11771 T11770 arg2Of plated,were
R10402 T11771 T11772 arg1Of plated,in
R10403 T11774 T11772 arg2Of plates,in
R10404 T11774 T11773 arg1Of plates,6-well
R10405 T11775 T11771 arg3Of at,plated
R10406 T11777 T11775 arg2Of cells/well,at
R10407 T11777 T11776 arg1Of cells/well,5×105
R10408 T11777 T11778 arg1Of cells/well,overnight
R10409 T11779 T11780 arg1Of Cells,were
R10410 T11779 T11781 arg2Of Cells,primed
R10411 T11779 T11788 arg2Of Cells,stimulated
R10412 T11779 T11795 arg2Of Cells,stimulated
R10413 T11781 T11782 arg1Of primed,with
R10414 T11781 T11784 arg1Of primed,for
R10415 T11781 T11787 arg1Of primed,and
R10416 T11783 T11782 arg2Of LPS,with
R10417 T11786 T11784 arg2Of hours,for
R10418 T11786 T11785 arg1Of hours,5
R10419 T11787 T11780 arg2Of and,were
R10420 T11788 T11789 arg1Of stimulated,with
R10421 T11788 T11791 arg1Of stimulated,for
R10422 T11788 T11794 arg1Of stimulated,or
R10423 T11790 T11789 arg2Of ATP,with
R10424 T11793 T11791 arg2Of hour,for
R10425 T11793 T11792 arg1Of hour,1
R10426 T11794 T11787 arg2Of or,and
R10427 T11795 T11794 arg2Of stimulated,or
R10428 T11795 T11796 arg1Of stimulated,with
R10429 T11795 T11799 arg1Of stimulated,for
R10430 T11798 T11796 arg2Of PAO1,with
R10431 T11798 T11797 arg1Of PAO1,live
R10432 T11801 T11799 arg2Of hours,for
R10433 T11801 T11800 arg1Of hours,3
R10434 T11802 T11803 arg1Of PBMCs,were
R10435 T11802 T11804 arg2Of PBMCs,stimulated
R10436 T11804 T11803 arg2Of stimulated,were
R10437 T11804 T11807 arg1Of stimulated,day
R10438 T11804 T11808 arg1Of stimulated,as
R10439 T11807 T11805 arg1Of day,the
R10440 T11807 T11806 arg1Of day,same
R10441 T11810 T11808 arg2Of donation,as
R10442 T11810 T11809 arg1Of donation,blood
R10443 T11811 T11812 arg1Of PBMCs,were
R10444 T11811 T11813 arg2Of PBMCs,seeded
R10445 T11811 T11837 arg2Of PBMCs,stimulated
R10446 T11811 T11854 arg2Of PBMCs,stimulated
R10447 T11813 T11814 arg1Of seeded,in
R10448 T11813 T11818 arg1Of seeded,at
R10449 T11813 T11835 arg1Of seeded,and
R10450 T11817 T11814 arg2Of plate,in
R10451 T11817 T11815 arg1Of plate,a
R10452 T11817 T11816 arg1Of plate,96-well
R10453 T11820 T11818 arg2Of density,at
R10454 T11820 T11819 arg1Of density,a
R10455 T11820 T11821 arg1Of density,of
R10456 T11820 T11828 arg1Of density,","
R10457 T11820 T11829 arg2Of density,primed
R10458 T11823 T11821 arg2Of cells/well,of
R10459 T11823 T11822 arg1Of cells/well,4.5×105
R10460 T11823 T11824 arg1Of cells/well,(
R10461 T11826 T11824 arg2Of cells/ml,(
R10462 T11826 T11825 arg1Of cells/ml,2.5×106
R10463 T11827 T11824 arg3Of ),(
R10464 T11829 T11830 arg1Of primed,with
R10465 T11829 T11832 arg1Of primed,for
R10466 T11831 T11830 arg2Of LPS,with
R10467 T11834 T11832 arg2Of hours,for
R10468 T11834 T11833 arg1Of hours,5
R10469 T11835 T11812 arg2Of and,were
R10470 T11837 T11838 arg1Of stimulated,with
R10471 T11837 T11853 arg1Of stimulated,or
R10472 T11839 T11838 arg2Of ATP,with
R10473 T11839 T11840 arg1Of ATP,for
R10474 T11839 T11850 arg1Of ATP,for
R10475 T11842 T11841 arg1Of hour,1
R10476 T11842 T11843 arg1Of hour,or
R10477 T11843 T11840 arg2Of or,for
R10478 T11844 T11843 arg2Of Poly,or
R10479 T11844 T11845 arg1Of Poly,(
R10480 T11846 T11845 arg2Of dA,(
R10481 T11846 T11847 arg1Of dA,:
R10482 T11846 T11848 arg1Of dA,dT
R10483 T11849 T11845 arg3Of ),(
R10484 T11852 T11850 arg2Of hours,for
R10485 T11852 T11851 arg1Of hours,3
R10486 T11853 T11835 arg2Of or,and
R10487 T11853 T11836 arg1Of or,then
R10488 T11854 T11853 arg2Of stimulated,or
R10489 T11854 T11855 arg1Of stimulated,with
R10490 T11854 T11858 arg1Of stimulated,for
R10491 T11857 T11855 arg2Of PAO1,with
R10492 T11857 T11856 arg1Of PAO1,live
R10493 T11860 T11858 arg2Of hours,for
R10494 T11860 T11859 arg1Of hours,3
R10495 T11862 T11861 arg1Of activity,Caspase-1
R10496 T11862 T11863 arg1Of activity,was
R10497 T11862 T11864 arg2Of activity,measured
R10498 T11864 T11863 arg2Of measured,was
R10499 T11865 T11864 arg3Of using,measured
R10500 T11866 T11865 arg2Of FLICA,using
R10501 T11866 T11867 arg1Of FLICA,(
R10502 T11866 T11871 arg1Of FLICA,","
R10503 T11869 T11867 arg2Of Technologies,(
R10504 T11869 T11868 arg1Of Technologies,Immunochemistry
R10505 T11870 T11867 arg3Of ),(
R10506 T11875 T11871 arg2Of probe,","
R10507 T11875 T11872 arg1Of probe,a
R10508 T11875 T11873 arg1Of probe,cell-permeable
R10509 T11875 T11874 arg1Of probe,fluorescent
R10510 T11875 T11876 arg1Of probe,(
R10511 T11875 T11879 arg1Of probe,that
R10512 T11875 T11880 arg1Of probe,binds
R10513 T11877 T11876 arg2Of FAM-YVAD-fmk,(
R10514 T11878 T11876 arg3Of ),(
R10515 T11882 T11880 arg2Of caspase-1,binds
R10516 T11882 T11881 arg1Of caspase-1,active
R10517 T11883 T11884 arg1Of Cells,were
R10518 T11883 T11885 arg2Of Cells,incubated
R10519 T11883 T11893 arg2Of Cells,stained
R10520 T11883 T11902 arg1Of Cells,identify
R10521 T11885 T11890 arg1Of incubated,at
R10522 T11885 T11892 arg1Of incubated,and
R10523 T11887 T11886 arg1Of hour,1
R10524 T11887 T11888 arg1Of hour,with
R10525 T11889 T11888 arg2Of FLICA,with
R10526 T11890 T11887 arg1Of at,hour
R10527 T11891 T11890 arg2Of 37°C,at
R10528 T11892 T11884 arg2Of and,were
R10529 T11893 T11892 arg2Of stained,and
R10530 T11893 T11894 arg1Of stained,with
R10531 T11893 T11901 modOf stained,to
R10532 T11897 T11894 arg2Of antibodies,with
R10533 T11897 T11895 arg1Of antibodies,PE-Cy7-conjugated
R10534 T11897 T11896 arg1Of antibodies,anti-CD14
R10535 T11897 T11898 arg1Of antibodies,(
R10536 T11899 T11898 arg2Of eBioscience,(
R10537 T11900 T11898 arg3Of ),(
R10538 T11902 T11901 arg1Of identify,to
R10539 T11903 T11902 arg2Of monocytes,identify
R10540 T11906 T11904 arg1Of strategy,The
R10541 T11906 T11905 arg1Of strategy,gating
R10542 T11906 T11907 arg1Of strategy,consisted
R10543 T11907 T11908 arg1Of consisted,of
R10544 T11911 T11908 arg2Of cells,of
R10545 T11911 T11909 arg1Of cells,including
R10546 T11911 T11910 arg1Of cells,live
R10547 T11911 T11912 arg1Of cells,that
R10548 T11911 T11913 arg1Of cells,were
R10549 T11911 T11916 arg1Of cells,which
R10550 T11911 T11917 arg1Of cells,were
R10551 T11911 T11919 arg2Of cells,analyzed
R10552 T11914 T11913 arg2Of CD14,were
R10553 T11914 T11915 arg1Of CD14,positive
R10554 T11919 T11917 arg2Of analyzed,were
R10555 T11919 T11918 arg1Of analyzed,subsequently
R10556 T11919 T11920 arg1Of analyzed,for
R10557 T11922 T11920 arg2Of frequency,for
R10558 T11922 T11921 arg1Of frequency,the
R10559 T11922 T11923 arg1Of frequency,of
R10560 T11926 T11923 arg2Of cells,of
R10561 T11926 T11924 arg1Of cells,FLICA
R10562 T11926 T11925 arg1Of cells,positive
R10744 T12145 T12143 arg1Of Assay,NF-κB/AP-1
R10745 T12145 T12144 arg1Of Assay,Activity
R10746 T12146 T12147 arg1Of Supernatants,from
R10747 T12146 T12151 arg1Of Supernatants,were
R10748 T12146 T12152 arg2Of Supernatants,incubated
R10749 T12146 T12162 arg2Of Supernatants,allowed
R10750 T12146 T12164 arg1Of Supernatants,develop
R10751 T12150 T12147 arg2Of cells,from
R10752 T12150 T12148 arg1Of cells,THP-1
R10753 T12150 T12149 arg1Of cells,reporter
R10754 T12152 T12153 arg1Of incubated,with
R10755 T12152 T12161 arg1Of incubated,and
R10756 T12155 T12153 arg2Of substrate,with
R10757 T12155 T12154 arg1Of substrate,Quanti-Blue
R10758 T12155 T12156 arg1Of substrate,(
R10759 T12155 T12159 arg1Of substrate,at
R10760 T12157 T12156 arg2Of Invivogen,(
R10761 T12158 T12156 arg3Of ),(
R10762 T12160 T12159 arg2Of 37°C,at
R10763 T12161 T12151 arg2Of and,were
R10764 T12162 T12161 arg2Of allowed,and
R10765 T12164 T12162 arg3Of develop,allowed
R10766 T12164 T12163 arg1Of develop,to
R10767 T12164 T12165 arg1Of develop,for
R10768 T12167 T12165 arg2Of hours,for
R10769 T12167 T12166 arg1Of hours,16–18
R10770 T12168 T12169 arg1Of Quanti-Blue,contains
R10771 T12169 T12175 arg1Of contains,and
R10772 T12171 T12169 arg2Of substrate,contains
R10773 T12171 T12170 arg1Of substrate,a
R10774 T12171 T12172 arg1Of substrate,for
R10775 T12174 T12172 arg2Of phosphatase,for
R10776 T12174 T12173 arg1Of phosphatase,alkaline
R10777 T12176 T12177 arg1Of changes,in
R10778 T12176 T12183 arg1Of changes,were
R10779 T12176 T12184 arg2Of changes,quantified
R10780 T12179 T12177 arg2Of amount,in
R10781 T12179 T12178 arg1Of amount,the
R10782 T12179 T12180 arg1Of amount,of
R10783 T12182 T12180 arg2Of activity,of
R10784 T12182 T12181 arg1Of activity,NF-κB/AP-1
R10785 T12184 T12175 arg2Of quantified,and
R10786 T12184 T12183 arg2Of quantified,were
R10787 T12184 T12192 modOf quantified,using
R10788 T12187 T12184 arg1Of density,quantified
R10789 T12187 T12185 arg2Of density,by
R10790 T12187 T12186 arg1Of density,optical
R10791 T12187 T12188 arg1Of density,(
R10792 T12187 T12191 arg2Of density,measured
R10793 T12189 T12188 arg2Of λ = 655,(
R10794 T12190 T12188 arg3Of ),(
R10795 T12198 T12193 arg1Of reader,a
R10796 T12198 T12194 arg1Of reader,SpectraMax
R10797 T12198 T12195 arg1Of reader,384
R10798 T12198 T12196 arg1Of reader,Plus
R10799 T12198 T12197 arg1Of reader,plate
R10800 T12198 T12199 arg1Of reader,and
R10801 T12199 T12192 arg2Of and,using
R10802 T12202 T12199 arg2Of software,and
R10803 T12202 T12200 arg1Of software,SoftMax
R10804 T12202 T12201 arg1Of software,Pro
R10805 T12202 T12203 arg1Of software,(
R10806 T12205 T12203 arg2Of Devices,(
R10807 T12205 T12204 arg1Of Devices,Molecular
R10808 T12206 T12203 arg3Of ),(
R10884 T12305 T12304 arg1Of strains,Bacterial
R10885 T12310 T12306 arg1Of PAO1,P.
R10886 T12310 T12307 arg1Of PAO1,aeruginosa
R10887 T12310 T12308 arg1Of PAO1,laboratory
R10888 T12310 T12309 arg1Of PAO1,strains
R10889 T12310 T12311 arg1Of PAO1,and
R10890 T12311 T12315 arg1Of and,were
R10891 T12311 T12316 arg2Of and,obtained
R10892 T12314 T12311 arg2Of mutant,and
R10893 T12314 T12312 arg1Of mutant,the
R10894 T12314 T12313 arg1Of mutant,PAO1ΔexsA
R10895 T12316 T12315 arg2Of obtained,were
R10896 T12316 T12317 arg1Of obtained,from
R10897 T12316 T12345 arg1Of obtained,until
R10898 T12321 T12318 arg1Of P.,Dr.
R10899 T12321 T12319 arg1Of P.,Robert
R10900 T12321 T12320 arg1Of P.,Hancock.
R10901 T12323 T12322 arg1Of strains,aeruginosa
R10902 T12323 T12327 modOf strains,were
R10903 T12324 T12325 arg1Of PAO1,and
R10904 T12325 T12327 arg1Of and,were
R10905 T12325 T12328 arg2Of and,grown
R10906 T12326 T12325 arg2Of PAO1ΔexsA,and
R10907 T12328 T12327 arg2Of grown,were
R10908 T12328 T12329 arg1Of grown,from
R10909 T12331 T12329 arg2Of cultures,from
R10910 T12331 T12330 arg1Of cultures,overnight
R10911 T12331 T12332 arg1Of cultures,in
R10912 T12334 T12332 arg2Of Bertani,in
R10913 T12334 T12333 arg1Of Bertani,Luria
R10914 T12336 T12335 arg2Of LB,(
R10915 T12337 T12335 arg3Of ),(
R10916 T12338 T12335 arg1Of broth,(
R10917 T12338 T12339 arg1Of broth,and
R10918 T12339 T12317 arg2Of and,from
R10919 T12339 T12321 arg1Of and,P.
R10920 T12339 T12323 arg1Of and,strains
R10921 T12340 T12339 arg2Of LB+streptomycin,and
R10922 T12340 T12341 arg1Of LB+streptomycin,(
R10923 T12343 T12341 arg2Of µg/ml,(
R10924 T12343 T12342 arg1Of µg/ml,150
R10925 T12344 T12341 arg3Of ),(
R10926 T12347 T12345 arg2Of phase,until
R10927 T12347 T12346 arg1Of phase,mid-logarithmic
R10928 T12348 T12349 arg1Of Cells,were
R10929 T12348 T12350 arg2Of Cells,washed
R10930 T12348 T12355 arg1Of Cells,resuspended
R10931 T12350 T12349 arg2Of washed,were
R10932 T12350 T12351 arg1Of washed,once
R10933 T12350 T12352 arg1Of washed,in
R10934 T12350 T12354 arg1Of washed,and
R10935 T12353 T12352 arg2Of PBS,in
R10936 T12355 T12354 arg2Of resuspended,and
R10937 T12355 T12356 arg1Of resuspended,in
R10938 T12355 T12358 arg1Of resuspended,to
R10939 T12357 T12356 arg2Of PBS,in
R10940 T12361 T12358 arg2Of density,to
R10941 T12361 T12359 arg1Of density,an
R10942 T12361 T12360 arg1Of density,optical
R10943 T12361 T12362 arg1Of density,of
R10944 T12363 T12362 arg2Of 0.5,of
R10945 T12363 T12364 arg1Of 0.5,(
R10946 T12366 T12364 arg2Of nm,(
R10947 T12366 T12365 arg1Of nm,λ = 600
R10948 T12367 T12364 arg3Of ),(
R10949 T12369 T12368 arg1Of prepare,To
R10950 T12371 T12369 arg2Of bacteria,prepare
R10951 T12371 T12370 arg1Of bacteria,heat-killed
R10952 T12374 T12369 arg1Of PAO1,prepare
R10953 T12374 T12373 arg1Of PAO1,live
R10954 T12374 T12375 arg1Of PAO1,was
R10955 T12374 T12376 arg2Of PAO1,resuspended
R10956 T12374 T12386 arg2Of PAO1,heated
R10957 T12376 T12377 arg1Of resuspended,in
R10958 T12376 T12379 arg1Of resuspended,to
R10959 T12376 T12385 arg1Of resuspended,and
R10960 T12378 T12377 arg2Of PBS,in
R10961 T12382 T12379 arg2Of density,to
R10962 T12382 T12380 arg1Of density,an
R10963 T12382 T12381 arg1Of density,optical
R10964 T12382 T12383 arg1Of density,of
R10965 T12384 T12383 arg2Of 0.5,of
R10966 T12385 T12368 modOf and,To
R10967 T12385 T12372 arg1Of and,","
R10968 T12385 T12375 arg2Of and,was
R10969 T12386 T12385 arg2Of heated,and
R10970 T12386 T12387 arg1Of heated,at
R10971 T12386 T12389 arg1Of heated,for
R10972 T12388 T12387 arg2Of 60°C,at
R10973 T12391 T12389 arg2Of hour,for
R10974 T12391 T12390 arg1Of hour,1
R10975 T12393 T12392 arg2Of stimulations,For
R10976 T12396 T12395 arg1Of PAO1,live
R10977 T12396 T12397 arg1Of PAO1,was
R10978 T12396 T12398 arg2Of PAO1,resuspended
R10979 T12396 T12409 arg2Of PAO1,diluted
R10980 T12398 T12397 arg2Of resuspended,was
R10981 T12398 T12399 arg1Of resuspended,to
R10982 T12398 T12407 arg1Of resuspended,and
R10983 T12399 T12405 arg1Of to,in
R10984 T12402 T12399 arg2Of density,to
R10985 T12402 T12400 arg1Of density,an
R10986 T12402 T12401 arg1Of density,optical
R10987 T12402 T12403 arg1Of density,of
R10988 T12404 T12403 arg2Of 0.5,of
R10989 T12406 T12405 arg2Of PBS,in
R10990 T12407 T12392 arg1Of and,For
R10991 T12407 T12394 arg1Of and,","
R10992 T12409 T12407 arg2Of diluted,and
R10993 T12409 T12408 arg1Of diluted,further
R10994 T12409 T12410 arg1Of diluted,in
R10995 T12412 T12410 arg2Of medium,in
R10996 T12412 T12411 arg1Of medium,culture
R10997 T12412 T12413 arg1Of medium,prior
R10998 T12413 T12414 arg1Of prior,to
R10999 T12415 T12414 arg2Of stimulation,to
R11000 T12415 T12416 modOf stimulation,to
R11001 T12417 T12416 arg1Of achieve,to
R11002 T12420 T12417 arg2Of multiplicity,achieve
R11003 T12420 T12418 arg1Of multiplicity,the
R11004 T12420 T12419 arg2Of multiplicity,desired
R11005 T12420 T12421 arg1Of multiplicity,of
R11006 T12422 T12421 arg2Of infection,of
R11007 T12424 T12423 arg1Of PAO1,Heat-killed
R11008 T12424 T12425 arg1Of PAO1,was
R11009 T12424 T12426 arg2Of PAO1,added
R11010 T12426 T12425 arg2Of added,was
R11011 T12426 T12427 arg1Of added,in
R11012 T12429 T12427 arg2Of volume,in
R11013 T12429 T12428 arg1Of volume,a
R11014 T12429 T12430 arg1Of volume,equivalent
R11015 T12430 T12431 arg1Of equivalent,to
R11016 T12432 T12431 arg2Of that,to
R11017 T12432 T12433 arg2Of that,used
R11018 T12433 T12434 modOf used,to
R11019 T12435 T12434 arg1Of achieve,to
R11020 T12437 T12435 arg2Of MOI,achieve
R11021 T12437 T12436 arg1Of MOI,an
R11022 T12437 T12438 arg1Of MOI,of
R11023 T12437 T12440 arg1Of MOI,for
R11024 T12439 T12438 arg2Of 1,of
R11025 T12442 T12440 arg2Of PAO1,for
R11026 T12442 T12441 arg1Of PAO1,live
R11180 T12611 T12610 arg1Of graphs,All
R11181 T12611 T12612 arg1Of graphs,display
R11182 T12611 T12618 arg1Of graphs,were
R11183 T12611 T12619 arg2Of graphs,generated
R11184 T12612 T12617 arg1Of display,and
R11185 T12616 T12612 arg2Of SEM,display
R11186 T12616 T12613 arg1Of SEM,the
R11187 T12616 T12614 arg1Of SEM,mean
R11188 T12616 T12615 arg1Of SEM,±
R11189 T12619 T12617 arg2Of generated,and
R11190 T12619 T12618 arg2Of generated,were
R11191 T12619 T12620 arg1Of generated,with
R11192 T12621 T12620 arg2Of Prism,with
R11193 T12621 T12622 arg1Of Prism,5
R11194 T12621 T12623 arg1Of Prism,(
R11195 T12624 T12623 arg2Of Graphpad,(
R11196 T12625 T12623 arg3Of ),(
R11197 T12627 T12626 arg1Of significance,Statistical
R11198 T12627 T12628 arg1Of significance,was
R11199 T12627 T12629 arg2Of significance,determined
R11200 T12629 T12628 arg2Of determined,was
R11201 T12629 T12630 arg1Of determined,by
R11202 T12629 T12640 arg1Of determined,where
R11203 T12631 T12630 arg2Of performing,by
R11204 T12635 T12634 arg1Of ANOVA,two-way
R11205 T12635 T12636 arg1Of ANOVA,and
R11206 T12636 T12631 arg2Of and,performing
R11207 T12636 T12632 arg1Of and,one
R11208 T12636 T12633 arg1Of and,or
R11209 T12638 T12637 arg1Of Bonferroni,the
R11210 T12639 T12636 arg2Of post-test,and
R11211 T12639 T12638 arg1Of post-test,Bonferroni
R11212 T12641 T12640 arg2Of applicable,where

sentences

Id Subject Object Predicate Lexical cue
T9309 23-39 Sentence denotes Ethics Statement
T9310 40-235 Sentence denotes Blood samples were obtained with informed written consent from control subjects and CF patients at the BC Children's Hospital. Consent was obtained for children by their parent or legal guardian.
T9311 236-319 Sentence denotes Subjects 7 years of age and older were required to provide informed assent as well.
T9312 320-394 Sentence denotes Protocols were approved by the Clinical Research Ethics Board (H09-01192).
T9454 396-408 Sentence denotes Cell Culture
T9455 409-660 Sentence denotes CF (IB3-1 and CuFi-1) and control (S9 and NuLi-1) cells were obtained from the American Type Culture Collection. IB3-1 cells were derived from a patient expressing the ΔF508 and W1282X mutations and CuFi-1 were derived from a ΔF508 homozygous patient.
T9456 661-844 Sentence denotes S9 cells are IB3-1 cells that have been transfected with CFTR using an adeno-associated viral vector and NuLi-1 cells were derived from a patient possessing a wild-type CFTR genotype.
T9457 845-975 Sentence denotes THP1-XBlue cells stably express a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB and AP-1 (Invivogen).
T9458 976-1066 Sentence denotes Cells were cultured as recommended by their respective suppliers using standard protocols.
T9459 1067-1225 Sentence denotes S9 and IB3-1 cells were cultured in basal LHC-8 (Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T9460 1226-1488 Sentence denotes NuLi-1/CuFi-1 cells were cultured in BEBM serum-free medium (Lonza) with supplement bullet kit (EGF, hydrocortisone, bovine pituitary extract, transferrin, bovine insulin, triiodothyronine, epinephrine, retinoic acid), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T9461 1489-1648 Sentence denotes PBMCs from CF patients and controls were cultured in RPMI-1640 (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, and 1 mM sodium pyruvate (complete RPMI).
T9462 1649-1797 Sentence denotes THP1-XBlue cells were cultured in complete RPMI with the addition of zeocin (100 µg/ml) to select for cells expressing the SEAP NF-κB/AP-1 reporter.
T9463 1798-1981 Sentence denotes Prior to stimulation, bronchial epithelial cell lines were plated in coated [62] 96-well plates (BD Biosciences) at 3×104 cells/well unless indicated, and allowed to adhere overnight.
T9464 1982-2159 Sentence denotes Plates for S9 and IB3-1 stimulations were coated in a mixture of bovine serum albumin (100 µg/ml), fibronectin (10 µg/ml), and bovine collage type I (30 µg/ml) (BD Biosciences).
T9465 2160-2250 Sentence denotes Plates for NuLi-1 and CuFi-1 were coated with collagen type IV (60 µg/ml) (Sigma Aldrich).
T9466 2251-2351 Sentence denotes PBMCs were plated in 96-well plates at a density of 1.5×105 cells/well in 200 µl (7.5×105 cells/mL).
T9467 2352-2571 Sentence denotes THP-1 reporter cells were differentiated into a macrophage-like phenotype using 50 ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich) for 24 hours at a density of 1×105 cells/well in 200 µl (5×105 cells/ml).
T9468 2572-2659 Sentence denotes Cells were washed with PBS and allowed to rest a further 42 hours prior to stimulation.
T10414 2661-2689 Sentence denotes CF and control subject PBMCs
T10415 2690-2849 Sentence denotes The diagnosis of CF was established by typical clinical features, increased sweat chloride concentrations (>60 mmol/l), and detection of CF-inducing mutations.
T10416 2850-3041 Sentence denotes All patients with CF were clinically stable at the time of blood donation, and we excluded any subjects who were receiving systemic corticosteroids due to potential immunomodulatory activity.
T10417 3042-3100 Sentence denotes Control samples were provided by healthy adult volunteers.
T10418 3101-3311 Sentence denotes In previously published work we have demonstrated that TLR-mediated inflammatory responses are stable in humans from birth to 60 years old [63], therefore did not age-match the CF patients and control subjects.
T10419 3312-3484 Sentence denotes Peripheral blood was collected in sodium heparin tubes (BD Biosciences) and PBMCs were isolated using density gradient centrifugation on Ficoll-Paque™ Plus (GE Healthcare).
T10420 3485-3579 Sentence denotes The layer containing PBMCs was isolated, washed twice in PBS and resuspended in complete RPMI.
T10421 3580-3682 Sentence denotes Cells were enumerated by trypan blue exclusion using the Countess automated cell counter (Invitrogen).
T10422 3683-3842 Sentence denotes For derivation of macrophages from monocytes, monocytes were allowed to adhere to plastic for 2 hours in RPMI 1640 after which non-adherent cells were removed.
T10423 3843-3945 Sentence denotes Monocytes were allowed to differentiate in RPMI 1640 supplemented with 10% human AB serum for 10 days.
T10861 3947-3991 Sentence denotes Cell stimulation and cytokine quantification
T10862 3992-4084 Sentence denotes Bronchial epithelial cells were plated and allowed to adhere overnight prior to stimulation.
T10863 4085-4230 Sentence denotes Bronchial epithelial cells were rested or primed with LPS for 5 hours and stimulated with live P. aeruginosa PAO1 or ATP for the times indicated.
T10864 4231-4353 Sentence denotes PBMCs and THP-1 reporter cells were either rested or primed with LPS (Invivogen) or heat-killed PAO1 overnight (16 hours).
T10865 4354-4505 Sentence denotes The next day the cells were challenged with live PAO1, PAO1ΔexsA, ATP (Invivogen), or Poly(dA:dT) (Sigma Aldrich) for the times indicated (see Fig. 1).
T10866 4506-4671 Sentence denotes For stimulations with Poly(dA:dT), lipofectamine LTX was used at a 1∶1 (w∶v) ratio of µg of DNA to µl of lipofectamine and was mixed 30 minutes prior to stimulation.
T10867 4672-4761 Sentence denotes The NF-κB inhibitor Bay11-7082 (Invivogen) was added to cultures 1 hour prior to priming.
T10868 4762-4851 Sentence denotes If no priming was involved, inhibitor was added 1 hour prior to inflammasome stimulation.
T10869 4852-4963 Sentence denotes The CFTR inhibitor CFTRinh172 (Sigma Aldrich) was added to cultures 18 hours prior to inflammasome stimulation.
T10870 4964-5074 Sentence denotes The caspase-1 inhibitor z-YVAD-fmk (Biovision) was added to cultures 1 hour prior to inflammasome stimulation.
T10871 5075-5263 Sentence denotes Supernatants were collected and stored at −20°C. Cytokines released into supernatants from PBMCs stimulated with inflammasome activators were quantified using sandwich ELISA (eBioscience).
T11380 5265-5279 Sentence denotes Immunoblotting
T11381 5280-5460 Sentence denotes 1×106 cells were seeded in 12-well plates, stimulated as indicated, and lysed in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific).
T11382 5461-5538 Sentence denotes Protein concentrations were determined by Bradford assay (Thermo Scientific).
T11383 5539-5659 Sentence denotes Lysates were resolved by electrophoreses on 10% SDS-polyacrylamide gels and transferred onto PVDF membranes (Millipore).
T11384 5660-5817 Sentence denotes Blots were blocked for 1 hour at room temperature and probed overnight at 4°C for pro-IL-1β (Santa Cruz), IκBα (Cell Signaling), or β-actin (Cell Signaling).
T11385 5818-5950 Sentence denotes Blots were subsequently probed with fluorescently-labeled secondary antibodies, IRDye® 680 or 800CW (LI-COR Biosciences) for 1 hour.
T11386 5951-6106 Sentence denotes Both blocking and probing steps were carried out in tris-buffered saline (G Biosciences) containing 5% bovine serum albumin and 0.1% TWEEN 20 (Calbiochem).
T11387 6107-6242 Sentence denotes Blots were imaged on a LI-COR Odyssey infrared imaging system (LI-COR Biosciences) and quantified using the included analysis software.
T11755 6244-6280 Sentence denotes Quantification of caspase-1 activity
T11756 6281-6367 Sentence denotes Bronchial epithelial cells were plated in 6-well plates at 5×105 cells/well overnight.
T11757 6368-6483 Sentence denotes Cells were primed with LPS for 5 hours and stimulated with ATP for 1 hour or stimulated with live PAO1 for 3 hours.
T11758 6484-6537 Sentence denotes PBMCs were stimulated the same day as blood donation.
T11759 6538-6766 Sentence denotes PBMCs were seeded in a 96-well plate at a density of 4.5×105 cells/well (2.5×106 cells/ml), primed with LPS for 5 hours and then stimulated with ATP for 1 hour or Poly(dA:dT) for 3 hours or stimulated with live PAO1 for 3 hours.
T11760 6767-6921 Sentence denotes Caspase-1 activity was measured using FLICA (Immunochemistry Technologies), a cell-permeable fluorescent probe (FAM-YVAD-fmk) that binds active caspase-1.
T11761 6922-7061 Sentence denotes Cells were incubated 1 hour with FLICA at 37°C and stained with PE-Cy7-conjugated anti-CD14 antibodies (eBioscience) to identify monocytes.
T11762 7062-7215 Sentence denotes The gating strategy consisted of including live cells that were CD14 positive which were subsequently analyzed for the frequency of FLICA positive cells.
T12140 7217-7242 Sentence denotes NF-κB/AP-1 Activity Assay
T12141 7243-7383 Sentence denotes Supernatants from THP-1 reporter cells were incubated with Quanti-Blue substrate (Invivogen) at 37°C and allowed to develop for 16–18 hours.
T12142 7384-7631 Sentence denotes Quanti-Blue contains a substrate for alkaline phosphatase and changes in the amount of NF-κB/AP-1 activity were quantified by optical density (λ = 655) measured using a SpectraMax 384 Plus plate reader and SoftMax Pro software (Molecular Devices).
T12298 7633-7650 Sentence denotes Bacterial strains
T12299 7651-7917 Sentence denotes P. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock. P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase.
T12300 7918-8013 Sentence denotes Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm).
T12301 8014-8139 Sentence denotes To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour.
T12302 8140-8328 Sentence denotes For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection.
T12303 8329-8429 Sentence denotes Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.
T12606 8431-8441 Sentence denotes Statistics
T12607 8442-8519 Sentence denotes All graphs display the mean ± SEM and were generated with Prism 5 (Graphpad).
T12608 8520-8641 Sentence denotes Statistical significance was determined by performing one or two-way ANOVA and the Bonferroni post-test where applicable.
T166 0-21 Sentence denotes Materials and Methods
T167 23-39 Sentence denotes Ethics Statement
T168 40-166 Sentence denotes Blood samples were obtained with informed written consent from control subjects and CF patients at the BC Children's Hospital.
T169 167-235 Sentence denotes Consent was obtained for children by their parent or legal guardian.
T170 236-319 Sentence denotes Subjects 7 years of age and older were required to provide informed assent as well.
T171 320-394 Sentence denotes Protocols were approved by the Clinical Research Ethics Board (H09-01192).
T172 396-408 Sentence denotes Cell Culture
T173 409-521 Sentence denotes CF (IB3-1 and CuFi-1) and control (S9 and NuLi-1) cells were obtained from the American Type Culture Collection.
T174 522-660 Sentence denotes IB3-1 cells were derived from a patient expressing the ΔF508 and W1282X mutations and CuFi-1 were derived from a ΔF508 homozygous patient.
T175 661-844 Sentence denotes S9 cells are IB3-1 cells that have been transfected with CFTR using an adeno-associated viral vector and NuLi-1 cells were derived from a patient possessing a wild-type CFTR genotype.
T176 845-975 Sentence denotes THP1-XBlue cells stably express a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB and AP-1 (Invivogen).
T177 976-1066 Sentence denotes Cells were cultured as recommended by their respective suppliers using standard protocols.
T178 1067-1225 Sentence denotes S9 and IB3-1 cells were cultured in basal LHC-8 (Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T179 1226-1488 Sentence denotes NuLi-1/CuFi-1 cells were cultured in BEBM serum-free medium (Lonza) with supplement bullet kit (EGF, hydrocortisone, bovine pituitary extract, transferrin, bovine insulin, triiodothyronine, epinephrine, retinoic acid), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T180 1489-1648 Sentence denotes PBMCs from CF patients and controls were cultured in RPMI-1640 (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, and 1 mM sodium pyruvate (complete RPMI).
T181 1649-1797 Sentence denotes THP1-XBlue cells were cultured in complete RPMI with the addition of zeocin (100 µg/ml) to select for cells expressing the SEAP NF-κB/AP-1 reporter.
T182 1798-1981 Sentence denotes Prior to stimulation, bronchial epithelial cell lines were plated in coated [62] 96-well plates (BD Biosciences) at 3×104 cells/well unless indicated, and allowed to adhere overnight.
T183 1982-2159 Sentence denotes Plates for S9 and IB3-1 stimulations were coated in a mixture of bovine serum albumin (100 µg/ml), fibronectin (10 µg/ml), and bovine collage type I (30 µg/ml) (BD Biosciences).
T184 2160-2250 Sentence denotes Plates for NuLi-1 and CuFi-1 were coated with collagen type IV (60 µg/ml) (Sigma Aldrich).
T185 2251-2351 Sentence denotes PBMCs were plated in 96-well plates at a density of 1.5×105 cells/well in 200 µl (7.5×105 cells/mL).
T186 2352-2571 Sentence denotes THP-1 reporter cells were differentiated into a macrophage-like phenotype using 50 ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich) for 24 hours at a density of 1×105 cells/well in 200 µl (5×105 cells/ml).
T187 2572-2659 Sentence denotes Cells were washed with PBS and allowed to rest a further 42 hours prior to stimulation.
T188 2661-2689 Sentence denotes CF and control subject PBMCs
T189 2690-2849 Sentence denotes The diagnosis of CF was established by typical clinical features, increased sweat chloride concentrations (>60 mmol/l), and detection of CF-inducing mutations.
T190 2850-3041 Sentence denotes All patients with CF were clinically stable at the time of blood donation, and we excluded any subjects who were receiving systemic corticosteroids due to potential immunomodulatory activity.
T191 3042-3100 Sentence denotes Control samples were provided by healthy adult volunteers.
T192 3101-3311 Sentence denotes In previously published work we have demonstrated that TLR-mediated inflammatory responses are stable in humans from birth to 60 years old [63], therefore did not age-match the CF patients and control subjects.
T193 3312-3484 Sentence denotes Peripheral blood was collected in sodium heparin tubes (BD Biosciences) and PBMCs were isolated using density gradient centrifugation on Ficoll-Paque™ Plus (GE Healthcare).
T194 3485-3579 Sentence denotes The layer containing PBMCs was isolated, washed twice in PBS and resuspended in complete RPMI.
T195 3580-3682 Sentence denotes Cells were enumerated by trypan blue exclusion using the Countess automated cell counter (Invitrogen).
T196 3683-3842 Sentence denotes For derivation of macrophages from monocytes, monocytes were allowed to adhere to plastic for 2 hours in RPMI 1640 after which non-adherent cells were removed.
T197 3843-3945 Sentence denotes Monocytes were allowed to differentiate in RPMI 1640 supplemented with 10% human AB serum for 10 days.
T198 3947-3991 Sentence denotes Cell stimulation and cytokine quantification
T199 3992-4084 Sentence denotes Bronchial epithelial cells were plated and allowed to adhere overnight prior to stimulation.
T200 4085-4230 Sentence denotes Bronchial epithelial cells were rested or primed with LPS for 5 hours and stimulated with live P. aeruginosa PAO1 or ATP for the times indicated.
T201 4231-4353 Sentence denotes PBMCs and THP-1 reporter cells were either rested or primed with LPS (Invivogen) or heat-killed PAO1 overnight (16 hours).
T202 4354-4505 Sentence denotes The next day the cells were challenged with live PAO1, PAO1ΔexsA, ATP (Invivogen), or Poly(dA:dT) (Sigma Aldrich) for the times indicated (see Fig. 1).
T203 4506-4671 Sentence denotes For stimulations with Poly(dA:dT), lipofectamine LTX was used at a 1∶1 (w∶v) ratio of µg of DNA to µl of lipofectamine and was mixed 30 minutes prior to stimulation.
T204 4672-4761 Sentence denotes The NF-κB inhibitor Bay11-7082 (Invivogen) was added to cultures 1 hour prior to priming.
T205 4762-4851 Sentence denotes If no priming was involved, inhibitor was added 1 hour prior to inflammasome stimulation.
T206 4852-4963 Sentence denotes The CFTR inhibitor CFTRinh172 (Sigma Aldrich) was added to cultures 18 hours prior to inflammasome stimulation.
T207 4964-5074 Sentence denotes The caspase-1 inhibitor z-YVAD-fmk (Biovision) was added to cultures 1 hour prior to inflammasome stimulation.
T208 5075-5123 Sentence denotes Supernatants were collected and stored at −20°C.
T209 5124-5263 Sentence denotes Cytokines released into supernatants from PBMCs stimulated with inflammasome activators were quantified using sandwich ELISA (eBioscience).
T210 5265-5279 Sentence denotes Immunoblotting
T211 5280-5460 Sentence denotes 1×106 cells were seeded in 12-well plates, stimulated as indicated, and lysed in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific).
T212 5461-5538 Sentence denotes Protein concentrations were determined by Bradford assay (Thermo Scientific).
T213 5539-5659 Sentence denotes Lysates were resolved by electrophoreses on 10% SDS-polyacrylamide gels and transferred onto PVDF membranes (Millipore).
T214 5660-5817 Sentence denotes Blots were blocked for 1 hour at room temperature and probed overnight at 4°C for pro-IL-1β (Santa Cruz), IκBα (Cell Signaling), or β-actin (Cell Signaling).
T215 5818-5950 Sentence denotes Blots were subsequently probed with fluorescently-labeled secondary antibodies, IRDye® 680 or 800CW (LI-COR Biosciences) for 1 hour.
T216 5951-6106 Sentence denotes Both blocking and probing steps were carried out in tris-buffered saline (G Biosciences) containing 5% bovine serum albumin and 0.1% TWEEN 20 (Calbiochem).
T217 6107-6242 Sentence denotes Blots were imaged on a LI-COR Odyssey infrared imaging system (LI-COR Biosciences) and quantified using the included analysis software.
T218 6244-6280 Sentence denotes Quantification of caspase-1 activity
T219 6281-6367 Sentence denotes Bronchial epithelial cells were plated in 6-well plates at 5×105 cells/well overnight.
T220 6368-6483 Sentence denotes Cells were primed with LPS for 5 hours and stimulated with ATP for 1 hour or stimulated with live PAO1 for 3 hours.
T221 6484-6537 Sentence denotes PBMCs were stimulated the same day as blood donation.
T222 6538-6766 Sentence denotes PBMCs were seeded in a 96-well plate at a density of 4.5×105 cells/well (2.5×106 cells/ml), primed with LPS for 5 hours and then stimulated with ATP for 1 hour or Poly(dA:dT) for 3 hours or stimulated with live PAO1 for 3 hours.
T223 6767-6921 Sentence denotes Caspase-1 activity was measured using FLICA (Immunochemistry Technologies), a cell-permeable fluorescent probe (FAM-YVAD-fmk) that binds active caspase-1.
T224 6922-7061 Sentence denotes Cells were incubated 1 hour with FLICA at 37°C and stained with PE-Cy7-conjugated anti-CD14 antibodies (eBioscience) to identify monocytes.
T225 7062-7215 Sentence denotes The gating strategy consisted of including live cells that were CD14 positive which were subsequently analyzed for the frequency of FLICA positive cells.
T226 7217-7242 Sentence denotes NF-κB/AP-1 Activity Assay
T227 7243-7383 Sentence denotes Supernatants from THP-1 reporter cells were incubated with Quanti-Blue substrate (Invivogen) at 37°C and allowed to develop for 16–18 hours.
T228 7384-7631 Sentence denotes Quanti-Blue contains a substrate for alkaline phosphatase and changes in the amount of NF-κB/AP-1 activity were quantified by optical density (λ = 655) measured using a SpectraMax 384 Plus plate reader and SoftMax Pro software (Molecular Devices).
T229 7633-7650 Sentence denotes Bacterial strains
T230 7651-7752 Sentence denotes P. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock.
T231 7753-7917 Sentence denotes P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase.
T232 7918-8013 Sentence denotes Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm).
T233 8014-8139 Sentence denotes To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour.
T234 8140-8328 Sentence denotes For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection.
T235 8329-8429 Sentence denotes Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.
T236 8431-8441 Sentence denotes Statistics
T237 8442-8519 Sentence denotes All graphs display the mean ± SEM and were generated with Prism 5 (Graphpad).
T238 8520-8641 Sentence denotes Statistical significance was determined by performing one or two-way ANOVA and the Bonferroni post-test where applicable.

2_test

Id Subject Object Predicate Lexical cue
22649552-21068401-88306746 1875-1877 21068401 denotes 62
22649552-17157070-88306747 3241-3243 17157070 denotes 63
T53749 1875-1877 21068401 denotes 62
T28787 3241-3243 17157070 denotes 63