PMC:3359311 / 15230-16298 JSONTXT

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5322","span":{"begin":24,"end":29},"obj":"P01584"},{"id":"T5323","span":{"begin":261,"end":266},"obj":"P01584"},{"id":"T5324","span":{"begin":458,"end":463},"obj":"P01584"},{"id":"T5325","span":{"begin":578,"end":582},"obj":"P25963"},{"id":"T14572","span":{"begin":721,"end":726},"obj":"P01584"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa \nIn addition to inhibition of NF-κB activity, Bay11-7082 can also directly inhibit the NLRP3 inflammasome [38]. To validate its use in this study as an NF-κB inhibitor, western blots for pro-IL-1β were performed alongside inhibition of CFTR activity by CFTRinh172 in response to PAO1 at 4 hours after stimulation (Fig. 7a). Our results indicate that Bay11-7082 prevents production of pro-IL-1β whereas CFTRinh172 does not seem to affect it. This was further corroborated by the ability Bay11-7082 to inhibit IκBα degradation at 0.5, 1, and 1.5 hours post PAO1 stimulation (Fig. 7b).\n10.1371/journal.pone.0037689.g007 Figure 7 Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa.\nPMA-differentiated THP-1 cells were treated with 10 µM CFTRinh172 or 20 µM Bay11-7082 and harvested after (A) 4 hours (n = 3) or (B) 0.5, 1, and 1.5 hours (n = 3) stimulation with PAO1. One representative blot is shown with a graph of the averaged fluorescence intensity values over 3 experiments.\n\nD"}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T5204","span":{"begin":570,"end":577},"obj":"Negative_regulation"},{"id":"T5205","span":{"begin":578,"end":582},"obj":"Protein"},{"id":"T5206","span":{"begin":583,"end":594},"obj":"Protein_catabolism"}],"relations":[{"id":"R4497","pred":"themeOf","subj":"T5205","obj":"T5206"},{"id":"R4498","pred":"themeOf","subj":"T5206","obj":"T5204"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa \nIn addition to inhibition of NF-κB activity, Bay11-7082 can also directly inhibit the NLRP3 inflammasome [38]. To validate its use in this study as an NF-κB inhibitor, western blots for pro-IL-1β were performed alongside inhibition of CFTR activity by CFTRinh172 in response to PAO1 at 4 hours after stimulation (Fig. 7a). Our results indicate that Bay11-7082 prevents production of pro-IL-1β whereas CFTRinh172 does not seem to affect it. This was further corroborated by the ability Bay11-7082 to inhibit IκBα degradation at 0.5, 1, and 1.5 hours post PAO1 stimulation (Fig. 7b).\n10.1371/journal.pone.0037689.g007 Figure 7 Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa.\nPMA-differentiated THP-1 cells were treated with 10 µM CFTRinh172 or 20 µM Bay11-7082 and harvested after (A) 4 hours (n = 3) or (B) 0.5, 1, and 1.5 hours (n = 3) stimulation with PAO1. One representative blot is shown with a graph of the averaged fluorescence intensity values over 3 experiments.\n\nD"}

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To validate its use in this study as an NF-κB inhibitor, western blots for pro-IL-1β were performed alongside inhibition of CFTR activity by CFTRinh172 in response to PAO1 at 4 hours after stimulation (Fig. 7a). Our results indicate that Bay11-7082 prevents production of pro-IL-1β whereas CFTRinh172 does not seem to affect it. This was further corroborated by the ability Bay11-7082 to inhibit IκBα degradation at 0.5, 1, and 1.5 hours post PAO1 stimulation (Fig. 7b).\n10.1371/journal.pone.0037689.g007 Figure 7 Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa.\nPMA-differentiated THP-1 cells were treated with 10 µM CFTRinh172 or 20 µM Bay11-7082 and harvested after (A) 4 hours (n = 3) or (B) 0.5, 1, and 1.5 hours (n = 3) stimulation with PAO1. One representative blot is shown with a graph of the averaged fluorescence intensity values over 3 experiments.\n\nD"}

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inhibits pro-IL-1β production in response to P. aeruginosa \nIn addition to inhibition of NF-κB activity, Bay11-7082 can also directly inhibit the NLRP3 inflammasome [38]. To validate its use in this study as an NF-κB inhibitor, western blots for pro-IL-1β were performed alongside inhibition of CFTR activity by CFTRinh172 in response to PAO1 at 4 hours after stimulation (Fig. 7a). Our results indicate that Bay11-7082 prevents production of pro-IL-1β whereas CFTRinh172 does not seem to affect it. This was further corroborated by the ability Bay11-7082 to inhibit IκBα degradation at 0.5, 1, and 1.5 hours post PAO1 stimulation (Fig. 7b).\n10.1371/journal.pone.0037689.g007 Figure 7 Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa.\nPMA-differentiated THP-1 cells were treated with 10 µM CFTRinh172 or 20 µM Bay11-7082 and harvested after (A) 4 hours (n = 3) or (B) 0.5, 1, and 1.5 hours (n = 3) stimulation with PAO1. One representative blot is shown with a graph of the averaged fluorescence intensity values over 3 experiments.\n\nD"}

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