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PMC:3359311 JSONTXT

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test2

Id Subject Object Predicate Lexical cue Negation Speculation
T875 3138-3156 Protein denotes Interleukin-1 beta
T876 3158-3163 Protein denotes IL-1β
T877 3399-3408 Positive_regulation denotes induction
T878 3453-3457 Protein denotes IL-6
T879 3568-3573 Protein denotes IL-1β
T880 3577-3585 Gene_expression denotes produced
T881 4377-4385 Positive_regulation denotes triggers
T882 4389-4397 Positive_regulation denotes increase
T883 4411-4416 Protein denotes IL-1β
T884 4418-4422 Protein denotes IL-6
T885 4587-4599 Positive_regulation denotes upregulation
T886 4659-4664 Protein denotes IL-1β
T887 4665-4675 Gene_expression denotes expression
T888 4724-4729 Protein denotes IL-1β
T889 4793-4798 Protein denotes IL-1β
T890 4803-4812 Positive_regulation denotes increased
T891 4990-4994 Protein denotes IL1B
T892 5149-5158 Positive_regulation denotes increases
T893 5162-5167 Protein denotes IL-1β
T894 5168-5178 Gene_expression denotes expression
T895 5182-5191 Localization denotes secretion
T896 5444-5453 Positive_regulation denotes increases
T897 5454-5463 Localization denotes secretion
T898 5467-5472 Protein denotes IL-1β
T899 5473-5487 Positive_regulation denotes in response to
T900 5676-5681 Protein denotes IL-1β
T2577 5788-5795 Gene_expression denotes produce
T2578 5819-5824 Protein denotes IL-1β true
T2579 5825-5839 Positive_regulation denotes in response to true
T2580 6229-6239 Positive_regulation denotes contribute
T2581 6247-6256 Positive_regulation denotes increased
T2582 6257-6262 Protein denotes IL-1β true
T2583 6263-6273 Gene_expression denotes production
T2584 6447-6452 Protein denotes IL-1β
T2585 6506-6515 Positive_regulation denotes increased
T2586 6585-6593 Positive_regulation denotes increase
T2587 6597-6602 Protein denotes IL-1β
T2588 6603-6613 Gene_expression denotes production
T2589 6805-6810 Protein denotes IL-1β
T2590 6812-6821 Gene_expression denotes producing
T9445 26904-26915 Protein denotes transferrin
T9446 26924-26931 Protein denotes insulin
T9447 27589-27602 Protein denotes serum albumin
T11752 32661-32665 Protein denotes CD14
T12138 32956-32976 Protein denotes alkaline phosphatase
T12791 7324-7334 Positive_regulation denotes contribute true
T12792 7338-7343 Protein denotes IL-1β
T12793 7344-7354 Gene_expression denotes production
T12794 7355-7369 Positive_regulation denotes in response to
T12795 7566-7571 Protein denotes IL-1β
T12796 7753-7758 Protein denotes IL-1β
T12797 7768-7778 Gene_expression denotes production
T12798 7807-7816 Localization denotes secretion
T12799 7817-7831 Positive_regulation denotes in response to
T12800 7849-7854 Protein denotes IL-1β
R707 T876 T875 equivalentTo IL-1β,Interleukin-1 beta
R708 T878 T877 themeOf IL-6,induction
R709 T879 T880 themeOf IL-1β,produced
R710 T882 T881 themeOf increase,triggers
R711 T883 T882 themeOf IL-1β,increase
R712 T884 T882 themeOf IL-6,increase
R713 T886 T887 themeOf IL-1β,expression
R714 T887 T885 themeOf expression,upregulation
R715 T889 T890 themeOf IL-1β,increased
R716 T893 T894 themeOf IL-1β,expression
R717 T894 T892 themeOf expression,increases
R718 T895 T892 themeOf secretion,increases
R719 T897 T899 themeOf secretion,in response to
R720 T897 T896 themeOf secretion,increases
R721 T898 T897 themeOf IL-1β,secretion
R2217 T2577 T2579 themeOf produce,in response to
R2218 T2578 T2577 themeOf IL-1β,produce
R2219 T2581 T2580 themeOf increased,contribute
R2220 T2582 T2583 themeOf IL-1β,production
R2221 T2583 T2581 themeOf production,increased
R2222 T2584 T2585 themeOf IL-1β,increased
R2223 T2587 T2588 themeOf IL-1β,production
R2224 T2588 T2586 themeOf production,increase
R11346 T12792 T12793 themeOf IL-1β,production
R11347 T12793 T12791 themeOf production,contribute
R11348 T12794 T12791 themeOf in response to,contribute
R11349 T12796 T12797 themeOf IL-1β,production
R11350 T12798 T12799 themeOf secretion,in response to
R11351 T12800 T12799 causeOf IL-1β,in response to

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T363 22-27 P01584 denotes IL-1β
T364 288-306 P01584 denotes Interleukin-1 beta
T365 308-313 P01584 denotes IL-1β
T366 380-385 P01584 denotes IL-1β
T367 471-476 P01584 denotes IL-1β
T368 569-574 P01584 denotes IL-1β
T369 757-762 P01584 denotes IL-1β
T370 1011-1016 P01584 denotes IL-1β
T371 1181-1186 P01584 denotes IL-1β
T372 1249-1254 P01584 denotes IL-1β
T373 1448-1453 P01584 denotes IL-1β
T374 1457-1461 P10145 denotes IL-8
T375 1612-1617 P01584 denotes IL-1β
T376 1702-1707 P01584 denotes IL-1β
T377 1842-1847 P01584 denotes IL-1β
T1641 3138-3156 P01584 denotes Interleukin-1 beta
T1642 3158-3163 P01584 denotes IL-1β
T1643 3453-3457 P05231 denotes IL-6
T1644 3462-3466 P10145 denotes IL-8
T1645 3568-3573 P01584 denotes IL-1β
T1646 3644-3649 P01584 denotes IL-1β
T1647 3655-3660 P01584 denotes IL-1β
T1648 3907-3912 P01584 denotes IL-1β
T1649 4411-4416 P01584 denotes IL-1β
T1650 4418-4422 P05231 denotes IL-6
T1651 4428-4432 P10145 denotes IL-8
T1652 4659-4664 P01584 denotes IL-1β
T1653 4724-4729 P01584 denotes IL-1β
T1654 4793-4798 P01584 denotes IL-1β
T1655 4990-4994 P01584 denotes IL1B
T1656 5162-5167 P01584 denotes IL-1β
T1657 5467-5472 P01584 denotes IL-1β
T1658 5676-5681 P01584 denotes IL-1β
T2800 5819-5824 P01584 denotes IL-1β
T2801 6257-6262 P01584 denotes IL-1β
T2802 6447-6452 P01584 denotes IL-1β
T2803 6597-6602 P01584 denotes IL-1β
T2804 6805-6810 P01584 denotes IL-1β
T2805 6842-6846 P10145 denotes IL-8
T3212 8498-8502 P05231 denotes IL-6
T3213 8627-8631 P05231 denotes IL-6
T3482 9425-9429 P08571 denotes CD14
T3483 9606-9610 P08571 denotes CD14
T3484 9636-9640 P08571 denotes CD14
T3907 10485-10490 P01584 denotes IL-1β
T3908 10962-10967 P01584 denotes IL-1β
T3909 10981-10985 P10145 denotes IL-8
T3910 11102-11107 P01584 denotes IL-1β
T3911 11232-11236 P26993 denotes exsA
T3912 11314-11319 P01584 denotes IL-1β
T3913 11443-11448 P01584 denotes IL-1β
T3914 11609-11614 P01584 denotes IL-1β
T3915 11618-11622 P10145 denotes IL-8
T4676 12413-12418 P01584 denotes IL-1β
T4677 12423-12427 P10145 denotes IL-8
T4678 12500-12505 P01584 denotes IL-1β
T4679 12510-12514 P10145 denotes IL-8
T4680 12606-12610 P05412 denotes AP-1
T4681 12746-12750 P05412 denotes AP-1
T4682 12797-12801 P10145 denotes IL-8
T4683 12848-12853 P01584 denotes IL-1β
T4684 12974-12978 P05412 denotes AP-1
T4685 13001-13005 P10145 denotes IL-8
T4686 13055-13060 P01584 denotes IL-1β
T4687 13273-13278 P01584 denotes IL-1β
T4688 13346-13351 P01584 denotes IL-1β
T4689 13532-13536 P25963 denotes IκBα
T4690 13588-13592 P05412 denotes AP-1
T4691 13657-13661 P10145 denotes IL-8
T4692 13685-13690 P01584 denotes IL-1β
T4693 13939-13944 P01584 denotes IL-1β
T4694 14040-14045 P01584 denotes IL-1β
T5322 15254-15259 P01584 denotes IL-1β
T5323 15491-15496 P01584 denotes IL-1β
T5324 15688-15693 P01584 denotes IL-1β
T5325 15808-15812 P25963 denotes IκBα
T5633 16343-16348 P01584 denotes IL-1β
T5634 16553-16558 P01584 denotes IL-1β
T5635 16766-16771 P01584 denotes IL-1β
T5636 16775-16779 P10145 denotes IL-8
T5637 16850-16855 P01584 denotes IL-1β
T5638 16978-16982 P10145 denotes IL-8
T7386 17830-17835 P01584 denotes IL-1β
T7387 18265-18270 P01584 denotes IL-1β
T7388 18520-18525 P01584 denotes IL-1β
T7389 18881-18886 P01584 denotes IL-1β
T7390 18975-18980 P01584 denotes IL-1β
T7391 19247-19252 P01584 denotes IL-1β
T7392 19369-19374 P01584 denotes IL-1β
T7393 19560-19565 P01584 denotes IL-1β
T7394 19581-19586 P01584 denotes IL-1β
T7395 19775-19780 P01584 denotes IL-1β
T7396 19935-19940 P01584 denotes IL-1β
T7397 20286-20291 P01584 denotes IL-1β
T7398 20342-20347 P01584 denotes IL-1β
T7399 20517-20522 P01584 denotes IL-1β
T7400 20696-20701 P01584 denotes IL-1β
T7401 20767-20772 P01584 denotes IL-1β
T7402 20860-20865 P01584 denotes IL-1β
T7403 21066-21071 P01584 denotes IL-1β
T7404 21307-21312 P01584 denotes IL-1β
T7405 21560-21565 P01584 denotes IL-1β
T7406 21818-21823 P01584 denotes IL-1β
T7407 21893-21898 P01584 denotes IL-1β
T7408 21960-21965 P01584 denotes IL-1β
T7409 22108-22113 P01584 denotes IL-1β
T7410 22221-22226 P01584 denotes IL-1β
T7411 22347-22352 P01584 denotes IL-1β
T7412 22432-22436 O14920 denotes IKKβ
T7413 22488-22493 P01584 denotes IL-1β
T7414 22593-22598 P01584 denotes IL-1β
T7415 22666-22670 P10145 denotes IL-8
T7416 22831-22836 P01584 denotes IL-1β
T7417 23044-23049 P01584 denotes IL-1β
T7418 23054-23058 P10145 denotes IL-8
T7419 23097-23102 P01584 denotes IL-1β
T7420 23107-23111 P10145 denotes IL-8
T7421 23253-23258 P01584 denotes IL-1β
T7422 23341-23345 P05412 denotes AP-1
T7423 23371-23375 P10145 denotes IL-8
T7424 24668-24672 P26993 denotes exsA
T7425 24713-24717 P26993 denotes exsA
T7426 24804-24809 P01584 denotes IL-1β
T7427 24902-24907 P01584 denotes IL-1β
T7428 25112-25117 P01584 denotes IL-1β
T7429 25241-25246 P01584 denotes IL-1β
T9879 26493-26497 P05412 denotes AP-1
T9880 27318-27322 P05412 denotes AP-1
T11550 31281-31286 P01584 denotes IL-1β
T11551 31301-31305 P25963 denotes IκBα
T11552 31327-31334 P60709 denotes β-actin
T11927 32544-32548 P08571 denotes CD14
T11928 32661-32665 P08571 denotes CD14
T12207 32758-32762 P05412 denotes AP-1
T12208 33012-33016 P05412 denotes AP-1
T12933 7338-7343 P01584 denotes IL-1β
T12934 7566-7571 P01584 denotes IL-1β
T12935 7753-7758 P01584 denotes IL-1β
T12936 7763-7767 P10145 denotes IL-8
T12937 7802-7806 P10145 denotes IL-8
T12938 7849-7854 P01584 denotes IL-1β
T13245 9401-9405 P05231 denotes IL-6
T13748 11952-11957 P01584 denotes IL-1β
T13749 12146-12150 P26993 denotes exsA
T13750 12235-12239 P26993 denotes exsA
T13751 12359-12364 P01584 denotes IL-1β
T13752 12373-12377 P10145 denotes IL-8
T14169 14134-14139 P01584 denotes IL-1β
T14170 14439-14443 P05412 denotes AP-1
T14171 14458-14462 P10145 denotes IL-8
T14172 14472-14477 P01584 denotes IL-1β
T14173 14720-14724 P05412 denotes AP-1
T14174 14739-14743 P10145 denotes IL-8
T14175 14753-14758 P01584 denotes IL-1β
T14176 15020-15025 P01584 denotes IL-1β
T14177 15034-15038 P10145 denotes IL-8
T14572 15951-15956 P01584 denotes IL-1β
T14806 17176-17181 P01584 denotes IL-1β
T14807 17373-17378 P01584 denotes IL-1β
T14808 17387-17391 P10145 denotes IL-8
T14809 17591-17596 P01584 denotes IL-1β
T14810 17732-17737 P01584 denotes IL-1β
T14811 17753-17757 P10145 denotes IL-8
T14812 17773-17777 P05412 denotes AP-1

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T49 707-714 Gene_expression denotes produce true
T50 746-756 Positive_regulation denotes stimulated
T37 13-21 Positive_regulation denotes Mediated
T38 22-27 Protein denotes IL-1β
T39 28-38 Gene_expression denotes Production
T40 288-306 Protein denotes Interleukin-1 beta
T41 308-313 Protein denotes IL-1β
T42 347-356 Localization denotes Secretion
T43 380-385 Protein denotes IL-1β
T44 386-394 Regulation denotes involves
T45 471-476 Protein denotes IL-1β
T46 560-568 Positive_regulation denotes mediated true
T47 569-574 Protein denotes IL-1β
T48 575-585 Gene_expression denotes production
T51 757-762 Protein denotes IL-1β
T52 985-993 Gene_expression denotes produced
T53 1011-1016 Protein denotes IL-1β
T54 1061-1065 Positive_regulation denotes upon
T55 1181-1186 Protein denotes IL-1β
T56 1187-1197 Gene_expression denotes production
T57 1203-1213 Positive_regulation denotes stimulated
T58 1249-1254 Protein denotes IL-1β
T59 1442-1447 Regulation denotes alter true
T60 1448-1453 Protein denotes IL-1β
T61 1462-1472 Gene_expression denotes production
T62 1473-1487 Positive_regulation denotes in response to
T63 1587-1594 Positive_regulation denotes induced
T64 1612-1617 Protein denotes IL-1β
T65 1664-1673 Positive_regulation denotes preserved
T66 1702-1707 Protein denotes IL-1β
T67 1708-1717 Localization denotes secretion
T68 1832-1841 Positive_regulation denotes increased
T69 1841-1847 Protein denotes IL-1β
T70 1848-1858 Gene_expression denotes production
T71 1877-1880 Positive_regulation denotes due true
T904 3138-3156 Protein denotes Interleukin-1 beta
T905 3158-3163 Protein denotes IL-1β
T906 3453-3457 Protein denotes IL-6
T907 3568-3573 Protein denotes IL-1β
T908 4377-4385 Positive_regulation denotes triggers
T909 4377-4385 Positive_regulation denotes triggers
T910 4389-4397 Positive_regulation denotes increase
T911 4389-4397 Positive_regulation denotes increase
T912 4411-4416 Protein denotes IL-1β
T913 4418-4422 Protein denotes IL-6
T914 4565-4574 Regulation denotes important
T915 4659-4664 Protein denotes IL-1β
T916 4665-4675 Gene_expression denotes expression
T917 4724-4729 Protein denotes IL-1β
T918 4793-4798 Protein denotes IL-1β
T919 4803-4812 Positive_regulation denotes increased
T920 4990-4994 Protein denotes IL1B
T921 5149-5158 Positive_regulation denotes increases
T922 5149-5158 Positive_regulation denotes increases
T923 5162-5167 Protein denotes IL-1β
T924 5168-5178 Gene_expression denotes expression
T925 5182-5191 Localization denotes secretion
T926 5444-5453 Positive_regulation denotes increases
T927 5454-5463 Localization denotes secretion
T928 5467-5472 Protein denotes IL-1β
T929 5676-5681 Protein denotes IL-1β
T2591 5788-5795 Gene_expression denotes produce
T2592 5819-5824 Protein denotes IL-1β
T2593 5825-5839 Positive_regulation denotes in response to true
T2594 6229-6239 Positive_regulation denotes contribute true
T2595 6247-6256 Positive_regulation denotes increased
T2596 6257-6262 Protein denotes IL-1β
T2597 6263-6273 Gene_expression denotes production
T2598 6447-6452 Protein denotes IL-1β
T2599 6506-6515 Positive_regulation denotes increased true
T2600 6585-6593 Positive_regulation denotes increase
T2601 6597-6602 Protein denotes IL-1β
T2602 6603-6613 Gene_expression denotes production
T2603 6805-6810 Protein denotes IL-1β
T3082 8474-8481 Regulation denotes altered true
T3083 8488-8497 Regulation denotes dependent
T3084 8498-8502 Protein denotes IL-6
T3085 8503-8513 Gene_expression denotes production
T3086 8514-8528 Positive_regulation denotes in response to
T3087 8618-8626 Negative_regulation denotes decrease true
T3088 8627-8631 Protein denotes IL-6
T3089 8632-8641 Localization denotes secretion
T3400 9606-9610 Protein denotes CD14
T3606 10456-10463 Gene_expression denotes produce
T3607 10464-10473 Positive_regulation denotes increased true
T3608 10485-10490 Protein denotes IL-1β
T3609 10933-10940 Gene_expression denotes produce
T3610 10941-10950 Positive_regulation denotes increased true
T3611 10962-10967 Protein denotes IL-1β
T3612 11066-11074 Negative_regulation denotes decrease
T3613 11102-11107 Protein denotes IL-1β
T3614 11108-11116 Gene_expression denotes produced
T3615 11129-11143 Positive_regulation denotes in response to
T3616 11232-11236 Protein denotes exsA
T3617 11289-11297 Gene_expression denotes produced
T3618 11309-11313 Negative_regulation denotes less
T3619 11314-11319 Protein denotes IL-1β
T3620 11429-11435 Positive_regulation denotes result true
T3621 11443-11448 Protein denotes IL-1β
T3622 11449-11459 Gene_expression denotes production true
T3623 11585-11594 Positive_regulation denotes increased true
T3624 11585-11594 Positive_regulation denotes increased true
T3625 11595-11605 Gene_expression denotes production
T3626 11595-11605 Gene_expression denotes production
T3627 11609-11614 Protein denotes IL-1β
T3628 11618-11622 Protein denotes IL-8
T4353 12400-12408 Positive_regulation denotes required
T4354 12413-12418 Protein denotes IL-1β
T4355 12428-12437 Regulation denotes responses
T4356 12473-12483 Regulation denotes dependence
T4357 12492-12499 Positive_regulation denotes induced
T4358 12500-12505 Protein denotes IL-1β
T4359 12515-12525 Gene_expression denotes production
T4360 12848-12853 Protein denotes IL-1β
T4361 12859-12867 Localization denotes secreted true
T4362 13055-13060 Protein denotes IL-1β
T4363 13061-13071 Gene_expression denotes production
T4364 13084-13093 Positive_regulation denotes augmented
T4365 13250-13260 Positive_regulation denotes sufficient true
T4366 13273-13278 Protein denotes IL-1β
T4367 13279-13288 Localization denotes secretion
T4368 13346-13351 Protein denotes IL-1β
T4369 13519-13528 Negative_regulation denotes inhibitor
T4370 13532-13536 Protein denotes IκBα
T4371 13537-13552 Phosphorylation denotes phosphorylation
T4372 13574-13581 Negative_regulation denotes reduced
T4373 13643-13653 Gene_expression denotes production
T4374 13685-13690 Protein denotes IL-1β
T4375 13711-13725 Positive_regulation denotes in response to
T4376 13926-13935 Regulation denotes modulator
T4377 13939-13944 Protein denotes IL-1β
T4378 13945-13955 Gene_expression denotes production
T4379 13995-14003 Positive_regulation denotes augments
T4380 14017-14025 Positive_regulation denotes mediated
T4381 14026-14036 Gene_expression denotes production
T4382 14040-14045 Protein denotes IL-1β
T5204 15800-15807 Negative_regulation denotes inhibit
T5205 15808-15812 Protein denotes IκBα
T5206 15813-15824 Protein_catabolism denotes degradation
T5486 16334-16342 Positive_regulation denotes increase true
T5487 16343-16348 Protein denotes IL-1β
T5488 16349-16359 Gene_expression denotes production
T5489 16546-16552 Regulation denotes alters true
T5490 16553-16558 Protein denotes IL-1β
T5491 16559-16569 Gene_expression denotes production
T5492 16760-16765 Regulation denotes alter true
T5493 16766-16771 Protein denotes IL-1β
T5494 16780-16790 Gene_expression denotes production
T5495 16850-16855 Protein denotes IL-1β
T5496 16856-16866 Gene_expression denotes production
T5947 17830-17835 Protein denotes IL-1β
T5948 17840-17849 Positive_regulation denotes increased
T5949 17926-17936 Gene_expression denotes production
T5950 17955-17963 Regulation denotes targeted true
T5951 18234-18241 Gene_expression denotes produce true
T5952 18265-18270 Protein denotes IL-1β
T5953 18520-18525 Protein denotes IL-1β
T5954 18867-18877 Gene_expression denotes production
T5955 18881-18886 Protein denotes IL-1β
T5956 18975-18980 Protein denotes IL-1β
T5957 19247-19252 Protein denotes IL-1β
T5958 19351-19365 Gene_expression denotes overproduction
T5959 19369-19374 Protein denotes IL-1β
T5960 19531-19538 Localization denotes secrete
T5961 19539-19548 Positive_regulation denotes increased true
T5962 19560-19565 Protein denotes IL-1β
T5963 19581-19586 Protein denotes IL-1β
T5964 19587-19597 Gene_expression denotes production
T5965 19618-19627 Positive_regulation denotes increased true
T5966 19765-19774 Positive_regulation denotes increased
T5967 19775-19780 Protein denotes IL-1β
T5968 19781-19791 Gene_expression denotes production
T5969 19935-19940 Protein denotes IL-1β
T5970 19941-19951 Gene_expression denotes production
T5971 19960-19969 Positive_regulation denotes increased true
T5972 20320-20338 Positive_regulation denotes This amplification
T5973 20342-20347 Protein denotes IL-1β
T5974 20348-20357 Localization denotes secretion
T5975 20507-20516 Positive_regulation denotes increased
T5976 20517-20522 Protein denotes IL-1β
T5977 20523-20532 Localization denotes secretion
T5978 20684-20692 Positive_regulation denotes increase
T5979 20696-20701 Protein denotes IL-1β
T5980 20702-20711 Localization denotes secretion
T5981 20719-20726 Regulation denotes absence true
T5982 20755-20763 Positive_regulation denotes increase true
T5983 20767-20772 Protein denotes IL-1β
T5984 20846-20856 Gene_expression denotes production
T5985 20860-20865 Protein denotes IL-1β
T5986 21038-21045 Negative_regulation denotes reduced
T5987 21058-21065 Gene_expression denotes produce
T5988 21066-21071 Protein denotes IL-1β
T5989 21551-21559 Positive_regulation denotes mediated
T5990 21560-21565 Protein denotes IL-1β
T5991 21566-21576 Gene_expression denotes production
T5992 21793-21800 Gene_expression denotes produce
T5993 21818-21823 Protein denotes IL-1β
T5994 21865-21872 Regulation denotes effects true
T5995 21893-21898 Protein denotes IL-1β
T5996 21899-21909 Gene_expression denotes production
T5997 21946-21956 Positive_regulation denotes contribute true
T5998 21960-21965 Protein denotes IL-1β
T5999 21966-21976 Gene_expression denotes production
T6000 22093-22100 Gene_expression denotes produce
T6001 22108-22113 Protein denotes IL-1β
T6002 22132-22143 Regulation denotes independent true true
T6003 22165-22169 Regulation denotes role
T6004 22221-22226 Protein denotes IL-1β
T6005 22227-22236 Localization denotes secretion
T6006 22478-22487 Positive_regulation denotes increases
T6007 22488-22493 Protein denotes IL-1β
T6008 22494-22503 Localization denotes secretion
T6009 22556-22565 Regulation denotes dual role
T6010 22579-22589 Regulation denotes regulation
T6011 22593-22598 Protein denotes IL-1β
T6012 23029-23038 Negative_regulation denotes inhibited
T6013 23044-23049 Protein denotes IL-1β
T6014 23059-23068 Localization denotes secretion
T6015 23097-23102 Protein denotes IL-1β
T6016 23112-23121 Regulation denotes responses
T6017 23141-23150 Regulation denotes dependent
T6018 23237-23243 Positive_regulation denotes induce true
T6019 23253-23258 Protein denotes IL-1β
T6020 23259-23267 Regulation denotes response
T6021 24694-24702 Negative_regulation denotes deletion
T6022 24713-24717 Protein denotes exsA
T6023 24804-24809 Protein denotes IL-1β
T6024 24842-24851 Regulation denotes depending
T6025 24842-24851 Regulation denotes depending
T6026 24902-24907 Protein denotes IL-1β
T6027 24908-24916 Regulation denotes response
T6028 24924-24926 Positive_regulation denotes up true
T6029 24930-24943 Negative_regulation denotes downregulated true
T6030 25103-25111 Positive_regulation denotes mediated
T6031 25112-25117 Protein denotes IL-1β
T6032 25118-25128 Gene_expression denotes production
T6033 25142-25156 Positive_regulation denotes in response to
T6034 25231-25240 Positive_regulation denotes increased
T6035 25241-25246 Protein denotes IL-1β
T6036 25260-25268 Regulation denotes response true true
T9451 26904-26915 Protein denotes transferrin
T9452 26924-26931 Protein denotes insulin
T9453 27589-27602 Protein denotes serum albumin
T11378 31301-31305 Protein denotes IκBα
T11379 31327-31334 Protein denotes β-actin
T11754 32661-32665 Protein denotes CD14
T12139 32956-32976 Protein denotes alkaline phosphatase
T12801 7324-7334 Positive_regulation denotes contribute true
T12802 7338-7343 Protein denotes IL-1β
T12803 7344-7354 Gene_expression denotes production
T12804 7355-7369 Positive_regulation denotes in response to
T12805 7566-7571 Protein denotes IL-1β
T12806 7753-7758 Protein denotes IL-1β
T12807 7768-7778 Gene_expression denotes production
T12808 7849-7854 Protein denotes IL-1β
T13115 9401-9405 Protein denotes IL-6
T13633 11923-11930 Gene_expression denotes produce
T13634 11931-11940 Positive_regulation denotes increased true
T13635 11952-11957 Protein denotes IL-1β
T13636 12138-12145 Negative_regulation denotes lacking
T13637 12146-12150 Protein denotes exsA
T13638 12227-12234 Negative_regulation denotes lacking
T13639 12235-12239 Protein denotes exsA
T13640 12359-12364 Protein denotes IL-1β
T13923 14108-14119 Positive_regulation denotes potentiates
T13924 14108-14119 Positive_regulation denotes potentiates
T13925 14134-14139 Protein denotes IL-1β
T13926 14140-14150 Gene_expression denotes production
T13927 14155-14164 Localization denotes secretion
T13928 14165-14169 Positive_regulation denotes upon
T13929 14165-14169 Positive_regulation denotes upon
T13930 14472-14477 Protein denotes IL-1β
T13931 14478-14487 Localization denotes secretion
T13932 14753-14758 Protein denotes IL-1β
T13933 14759-14768 Localization denotes secretion true
T13934 15020-15025 Protein denotes IL-1β
T14661 17167-17175 Positive_regulation denotes increase true
T14662 17176-17181 Protein denotes IL-1β
T14663 17182-17192 Gene_expression denotes production
T14664 17373-17378 Protein denotes IL-1β
T14665 17392-17402 Gene_expression denotes production true
T14666 17591-17596 Protein denotes IL-1β
T14667 17597-17607 Gene_expression denotes production true
T14668 17732-17737 Protein denotes IL-1β
T14669 17738-17747 Localization denotes secretion true
R23 T38 T39 themeOf IL-1β,Production
R24 T39 T37 themeOf Production,Mediated
R25 T41 T40 equivalentTo IL-1β,Interleukin-1 beta
R26 T42 T44 themeOf Secretion,involves
R27 T43 T42 themeOf IL-1β,Secretion
R28 T47 T48 themeOf IL-1β,production
R29 T48 T46 themeOf production,mediated
R30 T49 T50 themeOf produce,stimulated
R31 T51 T49 themeOf IL-1β,produce
R32 T52 T54 themeOf produced,upon
R33 T53 T52 themeOf IL-1β,produced
R34 T55 T56 themeOf IL-1β,production
R35 T56 T57 themeOf production,stimulated
R36 T60 T61 themeOf IL-1β,production
R37 T61 T62 themeOf production,in response to
R38 T62 T59 themeOf in response to,alter
R39 T64 T63 themeOf IL-1β,induced
R40 T66 T67 themeOf IL-1β,secretion
R41 T67 T65 themeOf secretion,preserved
R42 T68 T71 themeOf increased,due
R43 T69 T70 themeOf IL-1β,production
R44 T70 T68 themeOf production,increased
R722 T905 T904 equivalentTo IL-1β,Interleukin-1 beta
R723 T910 T908 themeOf increase,triggers
R724 T911 T909 themeOf increase,triggers
R725 T912 T911 themeOf IL-1β,increase
R726 T913 T910 themeOf IL-6,increase
R727 T915 T916 themeOf IL-1β,expression
R728 T916 T914 themeOf expression,important
R729 T918 T919 themeOf IL-1β,increased
R730 T923 T924 themeOf IL-1β,expression
R731 T923 T925 themeOf IL-1β,secretion
R732 T924 T922 themeOf expression,increases
R733 T925 T921 themeOf secretion,increases
R734 T927 T926 themeOf secretion,increases
R735 T928 T927 themeOf IL-1β,secretion
R2225 T2591 T2593 themeOf produce,in response to
R2226 T2592 T2591 themeOf IL-1β,produce
R2227 T2595 T2594 themeOf increased,contribute
R2228 T2596 T2597 themeOf IL-1β,production
R2229 T2597 T2595 themeOf production,increased
R2230 T2598 T2599 themeOf IL-1β,increased
R2231 T2601 T2602 themeOf IL-1β,production
R2232 T2602 T2600 themeOf production,increase
R2665 T3083 T3086 themeOf dependent,in response to
R2666 T3084 T3085 themeOf IL-6,production
R2667 T3085 T3083 themeOf production,dependent
R2668 T3086 T3082 themeOf in response to,altered
R2669 T3088 T3089 themeOf IL-6,secretion
R2670 T3089 T3087 themeOf secretion,decrease
R3115 T3606 T3607 themeOf produce,increased
R3116 T3608 T3606 themeOf IL-1β,produce
R3117 T3609 T3610 themeOf produce,increased
R3118 T3611 T3609 themeOf IL-1β,produce
R3119 T3612 T3615 themeOf decrease,in response to
R3120 T3613 T3614 themeOf IL-1β,produced
R3121 T3614 T3612 themeOf produced,decrease
R3122 T3617 T3618 themeOf produced,less
R3123 T3619 T3617 themeOf IL-1β,produced
R3124 T3621 T3622 themeOf IL-1β,production
R3125 T3622 T3620 themeOf production,result
R3126 T3625 T3623 themeOf production,increased
R3127 T3626 T3624 themeOf production,increased
R3128 T3627 T3625 themeOf IL-1β,production
R3129 T3628 T3626 themeOf IL-8,production
R3773 T4354 T4355 themeOf IL-1β,responses
R3774 T4355 T4353 themeOf responses,required
R3775 T4357 T4356 themeOf induced,dependence
R3776 T4358 T4359 themeOf IL-1β,production
R3777 T4359 T4357 themeOf production,induced
R3778 T4360 T4361 themeOf IL-1β,secreted
R3779 T4362 T4363 themeOf IL-1β,production
R3780 T4363 T4364 themeOf production,augmented
R3781 T4366 T4367 themeOf IL-1β,secretion
R3782 T4367 T4365 themeOf secretion,sufficient
R3783 T4369 T4372 causeOf inhibitor,reduced
R3784 T4370 T4371 themeOf IκBα,phosphorylation
R3785 T4371 T4369 themeOf phosphorylation,inhibitor
R3786 T4373 T4375 themeOf production,in response to
R3787 T4374 T4373 themeOf IL-1β,production
R3788 T4375 T4372 themeOf in response to,reduced
R3789 T4377 T4378 themeOf IL-1β,production
R3790 T4378 T4376 themeOf production,modulator
R3791 T4380 T4379 themeOf mediated,augments
R3792 T4381 T4380 themeOf production,mediated
R3793 T4382 T4381 themeOf IL-1β,production
R4497 T5205 T5206 themeOf IκBα,degradation
R4498 T5206 T5204 themeOf degradation,inhibit
R4746 T5487 T5488 themeOf IL-1β,production
R4747 T5488 T5486 themeOf production,increase
R4748 T5490 T5491 themeOf IL-1β,production
R4749 T5491 T5489 themeOf production,alters
R4750 T5493 T5494 themeOf IL-1β,production
R4751 T5494 T5492 themeOf production,alter
R4752 T5495 T5496 themeOf IL-1β,production
R5112 T5947 T5948 themeOf IL-1β,increased
R5113 T5947 T5949 themeOf IL-1β,production
R5114 T5949 T5950 themeOf production,targeted
R5115 T5952 T5951 themeOf IL-1β,produce
R5116 T5955 T5954 themeOf IL-1β,production
R5117 T5959 T5958 themeOf IL-1β,overproduction
R5118 T5960 T5961 themeOf secrete,increased
R5119 T5962 T5960 themeOf IL-1β,secrete
R5120 T5963 T5964 themeOf IL-1β,production
R5121 T5964 T5965 themeOf production,increased
R5122 T5967 T5968 themeOf IL-1β,production
R5123 T5968 T5966 themeOf production,increased
R5124 T5969 T5970 themeOf IL-1β,production
R5125 T5970 T5971 themeOf production,increased
R5126 T5973 T5974 themeOf IL-1β,secretion
R5127 T5974 T5972 themeOf secretion,This amplification
R5128 T5976 T5977 themeOf IL-1β,secretion
R5129 T5977 T5975 themeOf secretion,increased
R5130 T5978 T5981 themeOf increase,absence
R5131 T5979 T5980 themeOf IL-1β,secretion
R5132 T5980 T5978 themeOf secretion,increase
R5133 T5983 T5982 themeOf IL-1β,increase
R5134 T5985 T5984 themeOf IL-1β,production
R5135 T5987 T5986 themeOf produce,reduced
R5136 T5988 T5987 themeOf IL-1β,produce
R5137 T5990 T5991 themeOf IL-1β,production
R5138 T5991 T5989 themeOf production,mediated
R5139 T5993 T5992 themeOf IL-1β,produce
R5140 T5995 T5996 themeOf IL-1β,production
R5141 T5996 T5994 themeOf production,effects
R5142 T5998 T5999 themeOf IL-1β,production
R5143 T5999 T5997 themeOf production,contribute
R5144 T6000 T6002 themeOf produce,independent
R5145 T6001 T6000 themeOf IL-1β,produce
R5146 T6004 T6005 themeOf IL-1β,secretion
R5147 T6005 T6003 themeOf secretion,role
R5148 T6007 T6008 themeOf IL-1β,secretion
R5149 T6008 T6006 themeOf secretion,increases
R5150 T6010 T6009 themeOf regulation,dual role
R5151 T6011 T6010 themeOf IL-1β,regulation
R5152 T6013 T6014 themeOf IL-1β,secretion
R5153 T6014 T6012 themeOf secretion,inhibited
R5154 T6015 T6016 themeOf IL-1β,responses
R5155 T6016 T6017 themeOf responses,dependent
R5156 T6019 T6020 themeOf IL-1β,response
R5157 T6020 T6018 themeOf response,induce
R5158 T6022 T6021 themeOf exsA,deletion
R5159 T6026 T6027 themeOf IL-1β,response
R5160 T6027 T6028 themeOf response,up
R5161 T6027 T6029 themeOf response,downregulated
R5162 T6028 T6024 themeOf up,depending
R5163 T6029 T6025 themeOf downregulated,depending
R5164 T6030 T6033 themeOf mediated,in response to
R5165 T6031 T6032 themeOf IL-1β,production
R5166 T6032 T6030 themeOf production,mediated
R5167 T6035 T6036 themeOf IL-1β,response
R5168 T6036 T6034 themeOf response,increased
R11352 T12802 T12803 themeOf IL-1β,production
R11353 T12803 T12804 themeOf production,in response to
R11354 T12804 T12801 themeOf in response to,contribute
R11355 T12806 T12807 themeOf IL-1β,production
R12100 T13633 T13634 themeOf produce,increased
R12101 T13635 T13633 themeOf IL-1β,produce
R12102 T13637 T13636 themeOf exsA,lacking
R12103 T13639 T13638 themeOf exsA,lacking
R12352 T13923 T13929 themeOf potentiates,upon
R12353 T13924 T13928 themeOf potentiates,upon
R12354 T13925 T13926 themeOf IL-1β,production
R12355 T13925 T13927 themeOf IL-1β,secretion
R12356 T13926 T13924 themeOf production,potentiates
R12357 T13927 T13923 themeOf secretion,potentiates
R12358 T13930 T13931 themeOf IL-1β,secretion
R12359 T13932 T13933 themeOf IL-1β,secretion
R13025 T14662 T14663 themeOf IL-1β,production
R13026 T14663 T14661 themeOf production,increase
R13027 T14664 T14665 themeOf IL-1β,production
R13028 T14666 T14667 themeOf IL-1β,production
R13029 T14668 T14669 themeOf IL-1β,secretion

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T802 288-306 Protein denotes Interleukin-1 beta
T803 308-313 Protein denotes IL-1β
T804 337-345 Positive_regulation denotes mediator
T805 337-345 Positive_regulation denotes mediator
T806 380-385 Protein denotes IL-1β
T807 347-356 Localization denotes Secretion
T808 471-476 Protein denotes IL-1β
T809 757-762 Protein denotes IL-1β
T810 837-846 Protein denotes caspase-1
T811 707-714 Gene_expression denotes produce
T812 826-836 Positive_regulation denotes upregulate
T813 1011-1016 Protein denotes IL-1β
T814 1042-1051 Protein denotes caspase-1
T815 985-993 Gene_expression denotes produced
T816 1030-1041 Positive_regulation denotes upregulated
T817 1000-1007 Positive_regulation denotes amounts
T818 1156-1165 Protein denotes caspase-1
T819 1181-1186 Protein denotes IL-1β
T820 1166-1176 Positive_regulation denotes activation
T821 1187-1197 Gene_expression denotes production
T822 1166-1176 Positive_regulation denotes activation
T823 1249-1254 Protein denotes IL-1β
T824 1283-1288 Protein denotes NF-κB
T825 1255-1265 Gene_expression denotes production
T826 1270-1279 Positive_regulation denotes dependent
T827 1311-1319 Positive_regulation denotes enhanced
T828 1373-1377 Protein denotes CFTR
T829 1448-1453 Protein denotes IL-1β
T830 1457-1461 Protein denotes IL-8
T831 1359-1369 Negative_regulation denotes inhibition
T832 1462-1472 Gene_expression denotes production
T833 1462-1472 Gene_expression denotes production
T834 1442-1447 Positive_regulation denotes alter
T835 1442-1447 Positive_regulation denotes alter
T836 1612-1617 Protein denotes IL-1β
T837 1702-1707 Protein denotes IL-1β
T838 1743-1777 Protein denotes CF pathogen Pseudomonas aeruginosa
T839 1708-1717 Localization denotes secretion
T840 1708-1717 Localization denotes secretion
T841 1842-1847 Protein denotes IL-1β
T842 1909-1914 Protein denotes NF-κB
T843 1940-1944 Protein denotes CFTR
T844 1848-1858 Gene_expression denotes production
T845 1877-1880 Positive_regulation denotes due
T846 1897-1905 Positive_regulation denotes increase
T847 1932-1936 Negative_regulation denotes loss
T848 1832-1841 Positive_regulation denotes increased
T2513 2047-2098 Protein denotes cystic fibrosis transmembrane conductance regulator
T2514 2100-2104 Protein denotes CFTR
T2515 2839-2843 Protein denotes CFTR
T2516 2831-2838 Negative_regulation denotes lacking
T2517 3138-3156 Protein denotes Interleukin-1 beta
T2518 3158-3163 Protein denotes IL-1β
T2519 3189-3197 Positive_regulation denotes mediator
T2520 3189-3197 Positive_regulation denotes mediator
T2521 3453-3457 Protein denotes IL-6
T2522 3462-3466 Protein denotes IL-8
T2523 3399-3408 Positive_regulation denotes induction
T2524 3399-3408 Positive_regulation denotes induction
T2525 3543-3548 Protein denotes NF-κB
T2526 3568-3573 Protein denotes IL-1β
T2527 3577-3585 Gene_expression denotes produced
T2528 3907-3912 Protein denotes IL-1β
T2529 3917-3926 Protein denotes pro-IL-18
T2530 3968-3977 Localization denotes secretion
T2531 3968-3977 Localization denotes secretion
T2532 4043-4060 Protein denotes NLRP1 [12], NLRP3
T2533 4411-4416 Protein denotes IL-1β
T2534 4418-4422 Protein denotes IL-6
T2535 4428-4432 Protein denotes IL-8
T2536 4389-4397 Positive_regulation denotes increase
T2537 4389-4397 Positive_regulation denotes increase
T2538 4389-4397 Positive_regulation denotes increase
T2539 4533-4538 Protein denotes NF-κB
T2540 4556-4561 Protein denotes NF-κB
T2541 4659-4664 Protein denotes IL-1β
T2542 4665-4675 Gene_expression denotes expression
T2543 4587-4599 Positive_regulation denotes upregulation
T2544 4724-4729 Protein denotes IL-1β
T2545 4793-4798 Protein denotes IL-1β
T2546 4803-4812 Positive_regulation denotes increased
T2547 4990-4999 Protein denotes IL1B gene
T2548 5105-5109 Protein denotes CFTR
T2549 5162-5167 Protein denotes IL-1β
T2550 5263-5267 Protein denotes CFTR
T2551 5278-5283 Protein denotes NF-κB
T2552 5168-5178 Gene_expression denotes expression
T2553 5255-5259 Negative_regulation denotes loss
T2554 5284-5294 Positive_regulation denotes activation
T2555 5149-5158 Positive_regulation denotes increases
T2556 5268-5277 Positive_regulation denotes increases
T2557 5467-5472 Protein denotes IL-1β
T2558 5488-5493 Protein denotes NLRP3
T2559 5602-5607 Protein denotes NLRP3
T2560 5454-5463 Localization denotes secretion
T2561 5608-5618 Positive_regulation denotes activation
T2562 5444-5453 Positive_regulation denotes increases
T2563 5676-5681 Protein denotes IL-1β
T3057 5819-5824 Protein denotes IL-1β
T3058 5788-5795 Gene_expression denotes produce
T3059 6257-6262 Protein denotes IL-1β
T3060 6380-6414 Protein denotes inducers P. aeruginosa strain PAO1
T3061 6416-6420 Protein denotes PAO1
T3062 6263-6273 Gene_expression denotes production
T3063 6247-6256 Positive_regulation denotes increased
T3064 6447-6452 Protein denotes IL-1β
T3065 6597-6602 Protein denotes IL-1β
T3066 6506-6515 Positive_regulation denotes increased
T3067 6603-6613 Gene_expression denotes production
T3068 6585-6593 Positive_regulation denotes increase
T3069 6793-6810 Protein denotes recombinant IL-1β
T3070 6842-6846 Protein denotes IL-8
T3071 6848-6851 Protein denotes Fig
T3072 6812-6821 Gene_expression denotes producing
T3073 6812-6821 Gene_expression denotes producing
T3378 7992-8001 Protein denotes caspase-1
T3379 7981-7991 Positive_regulation denotes upregulate
T3380 8119-8128 Protein denotes caspase-1
T3381 8335-8344 Protein denotes caspase-1
T3382 8366-8371 Protein denotes NF-κB
T3383 8380-8398 Protein denotes Toll-like receptor
T3384 8400-8403 Protein denotes TLR
T3385 8464-8473 Protein denotes caspase-1
T3386 8482-8487 Protein denotes NF-κB
T3387 8498-8502 Protein denotes IL-6
T3388 8352-8362 Positive_regulation denotes activation
T3389 8450-8460 Negative_regulation denotes inhibition
T3390 8503-8513 Gene_expression denotes production
T3391 8326-8330 Regulation denotes role
T3392 8474-8481 Positive_regulation denotes altered
T3393 8488-8497 Positive_regulation denotes dependent
T3394 8572-8581 Protein denotes caspase-1
T3395 8627-8631 Protein denotes IL-6
T3396 8670-8677 Protein denotes Fig. 3c
T3397 8632-8641 Localization denotes secretion
T3398 8618-8626 Negative_regulation denotes decrease
T3572 9425-9429 Protein denotes CD14
T3573 9505-9514 Protein denotes caspase-1
T3574 9492-9501 Positive_regulation denotes increases
T3575 9606-9610 Protein denotes CD14
T3576 9636-9640 Protein denotes CD14
T3577 9731-9740 Protein denotes caspase-1
T3578 9783-9787 Protein denotes PAO1
T3579 9810-9817 Protein denotes Fig. 4a
T3580 9890-9897 Protein denotes Fig. 4b
T3581 9741-9751 Positive_regulation denotes activation
T3582 9719-9727 Positive_regulation denotes increase
T4279 10485-10490 Protein denotes IL-1β
T4280 10456-10463 Gene_expression denotes produce
T4281 10464-10473 Positive_regulation denotes increased
T4282 10592-10597 Protein denotes NF-κB
T4283 10582-10591 Positive_regulation denotes increased
T4284 10637-10646 Localization denotes secretion
T4285 10582-10591 Positive_regulation denotes increased
T4286 10784-10788 Protein denotes PAO1
T4287 10836-10841 Protein denotes NLRC4
T4288 10843-10848 Protein denotes NLRP3
T4289 10823-10831 Positive_regulation denotes activate
T4290 10823-10831 Positive_regulation denotes activate
T4291 10962-10967 Protein denotes IL-1β
T4292 10969-10976 Protein denotes Fig. 5a
T4293 10981-10985 Protein denotes IL-8
T4294 10987-10994 Protein denotes Fig. 5b
T4295 10933-10940 Gene_expression denotes produce
T4296 10933-10940 Gene_expression denotes produce
T4297 10933-10940 Gene_expression denotes produce
T4298 10933-10940 Gene_expression denotes produce
T4299 10941-10950 Positive_regulation denotes increased
T4300 10941-10950 Positive_regulation denotes increased
T4301 10941-10950 Positive_regulation denotes increased
T4302 10941-10950 Positive_regulation denotes increased
T4303 11102-11107 Protein denotes IL-1β
T4304 11108-11116 Gene_expression denotes produced
T4305 11232-11236 Protein denotes exsA
T4306 11238-11247 Protein denotes PAO1ΔexsA
T4307 11314-11319 Protein denotes IL-1β
T4308 11345-11349 Protein denotes PAO1
T4309 11226-11231 Negative_regulation denotes lacks
T4310 11226-11231 Negative_regulation denotes lacks
T4311 11289-11297 Gene_expression denotes produced
T4312 11443-11448 Protein denotes IL-1β
T4313 11449-11459 Gene_expression denotes production
T4314 11429-11435 Positive_regulation denotes result
T4315 11609-11614 Protein denotes IL-1β
T4316 11618-11622 Protein denotes IL-8
T4317 11703-11708 Protein denotes NF-κB
T4318 11595-11605 Gene_expression denotes production
T4319 11595-11605 Gene_expression denotes production
T4320 11585-11594 Positive_regulation denotes increased
T4321 11585-11594 Positive_regulation denotes increased
T4322 11774-11783 Protein denotes caspase-1
T5117 12380-12385 Protein denotes NF-κB
T5118 12413-12418 Protein denotes IL-1β
T5119 12423-12427 Protein denotes IL-8
T5120 12386-12396 Positive_regulation denotes activation
T5121 12428-12437 Regulation denotes responses
T5122 12428-12437 Regulation denotes responses
T5123 12487-12491 Protein denotes PAO1
T5124 12500-12505 Protein denotes IL-1β
T5125 12510-12514 Protein denotes IL-8
T5126 12529-12534 Protein denotes NF-κB
T5127 12596-12601 Protein denotes NF-κB
T5128 12606-12628 Protein denotes AP-1 response elements
T5129 12492-12499 Positive_regulation denotes induced
T5130 12515-12525 Gene_expression denotes production
T5131 12515-12525 Gene_expression denotes production
T5132 12535-12545 Positive_regulation denotes activation
T5133 12473-12483 Positive_regulation denotes dependence
T5134 12473-12483 Positive_regulation denotes dependence
T5135 12473-12483 Positive_regulation denotes dependence
T5136 12473-12483 Positive_regulation denotes dependence
T5137 12492-12499 Positive_regulation denotes induced
T5138 12492-12499 Positive_regulation denotes induced
T5139 12704-12708 Protein denotes PAO1
T5140 12740-12745 Protein denotes NF-κB
T5141 12746-12750 Protein denotes AP-1
T5142 12763-12770 Protein denotes Fig. 6a
T5143 12797-12811 Protein denotes IL-8 secretion
T5144 12813-12820 Protein denotes Fig. 6b
T5145 12848-12853 Protein denotes IL-1β
T5146 12869-12876 Protein denotes Fig. 6c
T5147 12709-12717 Gene_expression denotes produced
T5148 12709-12717 Gene_expression denotes produced
T5149 12751-12761 Positive_regulation denotes activation
T5150 12751-12761 Positive_regulation denotes activation
T5151 12837-12844 Positive_regulation denotes amounts
T5152 12859-12867 Localization denotes secreted
T5153 12932-12936 Protein denotes PAO1
T5154 12968-12973 Protein denotes NF-κB
T5155 12974-12978 Protein denotes AP-1
T5156 12989-12996 Protein denotes Fig. 6a
T5157 13001-13015 Protein denotes IL-8 secretion
T5158 13017-13024 Protein denotes Fig. 6b
T5159 12959-12967 Positive_regulation denotes increase
T5160 12959-12967 Positive_regulation denotes increase
T5161 12959-12967 Positive_regulation denotes increase
T5162 12959-12967 Positive_regulation denotes increase
T5163 13055-13060 Protein denotes IL-1β
T5164 13140-13144 Protein denotes PAO1
T5165 13184-13188 Protein denotes PAO1
T5166 13190-13197 Protein denotes Fig. 6c
T5167 13061-13071 Gene_expression denotes production
T5168 13084-13093 Positive_regulation denotes augmented
T5169 13220-13225 Protein denotes NF-κB
T5170 13273-13278 Protein denotes IL-1β
T5171 13315-13320 Protein denotes NF-κB
T5172 13346-13351 Protein denotes IL-1β
T5173 13226-13236 Positive_regulation denotes activation
T5174 13279-13288 Localization denotes secretion
T5175 13352-13362 Gene_expression denotes production
T5176 13335-13345 Positive_regulation denotes augmenting
T5177 13441-13446 Protein denotes NF-κB
T5178 13492-13497 Protein denotes NF-κB
T5179 13532-13536 Protein denotes IκBα
T5180 13582-13587 Protein denotes NF-κB
T5181 13588-13592 Protein denotes AP-1
T5182 13685-13690 Protein denotes IL-1β
T5183 13752-13756 Protein denotes PAO1
T5184 13478-13488 Negative_regulation denotes inhibition
T5185 13537-13552 Phosphorylation denotes phosphorylation
T5186 13643-13653 Gene_expression denotes production
T5187 13519-13528 Negative_regulation denotes inhibitor
T5188 13593-13603 Positive_regulation denotes activation
T5189 13632-13642 Positive_regulation denotes subsequent
T5190 13574-13581 Negative_regulation denotes reduced
T5191 13904-13909 Protein denotes NF-κB
T5192 13939-13944 Protein denotes IL-1β
T5193 13989-13994 Protein denotes NF-κB
T5194 14040-14045 Protein denotes IL-1β
T5195 13945-13955 Gene_expression denotes production
T5196 13975-13985 Positive_regulation denotes activation
T5197 14026-14036 Gene_expression denotes production
T5198 13926-13935 Positive_regulation denotes modulator
T5199 13965-13974 Positive_regulation denotes increased
T5200 13995-14003 Positive_regulation denotes augments
T5457 15330-15335 Protein denotes NF-κB
T5458 15387-15405 Protein denotes NLRP3 inflammasome
T5459 15316-15326 Negative_regulation denotes inhibition
T5460 15375-15382 Negative_regulation denotes inhibit
T5461 15452-15457 Protein denotes NF-κB
T5462 15536-15540 Protein denotes CFTR
T5463 15553-15563 Protein denotes CFTRinh172
T5464 15579-15583 Protein denotes PAO1
T5465 15614-15621 Protein denotes Fig. 7a
T5466 15458-15467 Negative_regulation denotes inhibitor
T5467 15522-15532 Negative_regulation denotes inhibition
T5468 15808-15812 Protein denotes IκBα
T5469 15855-15859 Protein denotes PAO1
T5470 15873-15880 Protein denotes Fig. 7b
T5471 15813-15824 Protein_catabolism denotes degradation
T5472 15860-15871 Positive_regulation denotes stimulation
T5473 15800-15807 Negative_regulation denotes inhibit
T5474 15800-15807 Negative_regulation denotes inhibit
T5823 16311-16315 Protein denotes CFTR
T5824 16343-16348 Protein denotes IL-1β
T5825 16297-16307 Negative_regulation denotes Disruption
T5826 16349-16359 Gene_expression denotes production
T5827 16334-16342 Positive_regulation denotes increase
T5828 16472-16490 Protein denotes NLRP3 inflammasome
T5829 16529-16533 Protein denotes CFTR
T5830 16553-16558 Protein denotes IL-1β
T5831 16653-16657 Protein denotes CFTR
T5832 16534-16545 Positive_regulation denotes dysfunction
T5833 16559-16569 Gene_expression denotes production
T5834 16546-16552 Positive_regulation denotes alters
T5835 16766-16771 Protein denotes IL-1β
T5836 16775-16779 Protein denotes IL-8
T5837 16780-16790 Gene_expression denotes production
T5838 16780-16790 Gene_expression denotes production
T5839 16760-16765 Regulation denotes alter
T5840 16760-16765 Regulation denotes alter
T5841 16850-16855 Protein denotes IL-1β
T5842 16954-16964 Protein denotes CFTRinh172
T5843 16966-16969 Protein denotes Fig
T5844 16856-16866 Gene_expression denotes production
T5845 16978-16982 Protein denotes IL-8
T5846 16984-16991 Protein denotes Fig. 8e
T5847 16997-17002 Protein denotes NF-κB
T5848 17013-17020 Protein denotes Fig. 8f
T9156 17830-17835 Protein denotes IL-1β
T9157 17840-17849 Positive_regulation denotes increased
T9158 17926-17936 Gene_expression denotes production
T9159 18265-18270 Protein denotes IL-1β
T9160 18313-18322 Protein denotes caspase-1
T9161 18349-18353 Protein denotes PAO1
T9162 18234-18241 Gene_expression denotes produce
T9163 18323-18333 Positive_regulation denotes activation
T9164 18301-18309 Positive_regulation denotes increase
T9165 18520-18525 Protein denotes IL-1β
T9166 18510-18516 Gene_expression denotes source
T9167 18527-18531 Protein denotes CFTR
T9168 18535-18544 Gene_expression denotes expressed
T9169 18881-18886 Protein denotes IL-1β
T9170 18888-18891 Protein denotes Fig
T9171 18975-18980 Protein denotes IL-1β
T9172 18867-18877 Gene_expression denotes production
T9173 19247-19252 Protein denotes IL-1β
T9174 19369-19374 Protein denotes IL-1β
T9175 19351-19365 Gene_expression denotes overproduction
T9176 19351-19365 Positive_regulation denotes overproduction
T9177 19560-19565 Protein denotes IL-1β
T9178 19581-19586 Protein denotes IL-1β
T9179 19601-19609 Protein denotes CF PBMCs
T9180 19531-19538 Localization denotes secrete
T9181 19539-19548 Positive_regulation denotes increased
T9182 19587-19597 Gene_expression denotes production
T9183 19587-19597 Gene_expression denotes production
T9184 19618-19627 Positive_regulation denotes increased
T9185 19618-19627 Positive_regulation denotes increased
T9186 19775-19780 Protein denotes IL-1β
T9187 19795-19803 Protein denotes CF PBMCs
T9188 19781-19791 Gene_expression denotes production
T9189 19781-19791 Gene_expression denotes production
T9190 19765-19774 Positive_regulation denotes increased
T9191 19765-19774 Positive_regulation denotes increased
T9192 19935-19940 Protein denotes IL-1β
T9193 20072-20077 Protein denotes NF-κB
T9194 19941-19951 Gene_expression denotes production
T9195 19960-19969 Positive_regulation denotes increased
T9196 20078-20088 Positive_regulation denotes activation
T9197 20109-20113 Protein denotes CFTR
T9198 20165-20170 Protein denotes NF-κB
T9199 20096-20105 Negative_regulation denotes deficient
T9200 20140-20149 Positive_regulation denotes increased
T9201 20342-20347 Protein denotes IL-1β
T9202 20348-20357 Localization denotes secretion
T9203 20517-20522 Protein denotes IL-1β
T9204 20523-20532 Localization denotes secretion
T9205 20507-20516 Positive_regulation denotes increased
T9206 20618-20623 Protein denotes PBMCs
T9207 20644-20655 Protein denotes basal NF-κB
T9208 20696-20701 Protein denotes IL-1β
T9209 20624-20633 Gene_expression denotes expressed
T9210 20624-20633 Gene_expression denotes expressed
T9211 20634-20643 Positive_regulation denotes increased
T9212 20702-20711 Localization denotes secretion
T9213 20684-20692 Positive_regulation denotes increase
T9214 20767-20772 Protein denotes IL-1β
T9215 20755-20763 Positive_regulation denotes increase
T9216 20860-20865 Protein denotes IL-1β
T9217 20846-20856 Gene_expression denotes production
T9218 21003-21013 Protein denotes ΔF508 CFTR
T9219 21066-21071 Protein denotes IL-1β
T9220 21114-21119 Protein denotes NF-κB
T9221 21058-21065 Gene_expression denotes produce
T9222 21120-21130 Positive_regulation denotes activation
T9223 21038-21045 Negative_regulation denotes reduced
T9224 21098-21104 Positive_regulation denotes induce
T9225 21266-21271 Protein denotes ΔF508
T9226 21272-21276 Protein denotes CFTR
T9227 21307-21312 Protein denotes IL-1β
T9228 21360-21364 Protein denotes CFTR
T9229 21380-21410 Protein denotes Burkholderia cenocepacia K56-2
T9230 21255-21265 Gene_expression denotes expressing
T9231 21255-21265 Gene_expression denotes expressing
T9232 21277-21285 Gene_expression denotes produced
T9233 21342-21352 Gene_expression denotes expressing
T9234 21701-21705 Protein denotes CFTR
T9235 21687-21697 Gene_expression denotes expression
T9236 21818-21823 Protein denotes IL-1β
T9237 21876-21880 Protein denotes CFTR
T9238 21893-21898 Protein denotes IL-1β
T9239 21793-21800 Gene_expression denotes produce
T9240 21899-21909 Gene_expression denotes production
T9241 21865-21872 Regulation denotes effects
T9242 21960-21965 Protein denotes IL-1β
T9243 21966-21976 Gene_expression denotes production
T9244 22108-22113 Protein denotes IL-1β
T9245 22122-22131 Protein denotes caspase-1
T9246 22093-22100 Gene_expression denotes produce
T9247 22093-22100 Gene_expression denotes produce
T9248 22173-22178 Protein denotes NF-κB
T9249 22221-22226 Protein denotes IL-1β
T9250 22179-22189 Positive_regulation denotes activation
T9251 22227-22236 Localization denotes secretion
T9252 22305-22310 Protein denotes NF-κB
T9253 22311-22321 Positive_regulation denotes activation
T9254 22296-22300 Regulation denotes role
T9255 22432-22436 Protein denotes IKKβ
T9256 22460-22465 Protein denotes NF-κB
T9257 22488-22493 Protein denotes IL-1β
T9258 22570-22575 Protein denotes NF-κB
T9259 22593-22598 Protein denotes IL-1β
T9260 22420-22428 Negative_regulation denotes deletion
T9261 22466-22476 Positive_regulation denotes activation
T9262 22494-22503 Localization denotes secretion
T9263 22579-22589 Regulation denotes regulation
T9264 22478-22487 Positive_regulation denotes increases
T9265 22561-22565 Positive_regulation denotes role
T9266 22666-22670 Protein denotes IL-8
T9267 22711-22716 Protein denotes NF-κB
T9268 22870-22875 Protein denotes NF-κB
T9269 22857-22866 Regulation denotes dependent
T9270 22988-22993 Protein denotes NF-κB
T9271 23044-23049 Protein denotes IL-1β
T9272 23054-23058 Protein denotes IL-8
T9273 23029-23038 Negative_regulation denotes inhibited
T9274 23059-23068 Localization denotes secretion
T9275 23059-23068 Localization denotes secretion
T9276 23029-23038 Negative_regulation denotes inhibited
T9277 23029-23038 Negative_regulation denotes inhibited
T9278 23097-23102 Protein denotes IL-1β
T9279 23107-23111 Protein denotes IL-8
T9280 23156-23161 Protein denotes NF-κB
T9281 23162-23172 Positive_regulation denotes activation
T9282 23253-23258 Protein denotes IL-1β
T9283 23335-23340 Protein denotes NF-κB
T9284 23341-23345 Protein denotes AP-1
T9285 23358-23365 Protein denotes Fig. 6a
T9286 23371-23385 Protein denotes IL-8 secretion
T9287 23387-23394 Protein denotes Fig. 6b
T9288 23259-23267 Positive_regulation denotes response
T9289 23346-23356 Positive_regulation denotes activation
T9290 23346-23356 Positive_regulation denotes activation
T9291 23346-23356 Positive_regulation denotes activation
T9292 23346-23356 Positive_regulation denotes activation
T9293 23237-23243 Positive_regulation denotes induce
T9294 23317-23326 Positive_regulation denotes generated
T9295 23317-23326 Positive_regulation denotes generated
T9296 23317-23326 Positive_regulation denotes generated
T9297 23317-23326 Positive_regulation denotes generated
T9298 24269-24273 Protein denotes CFTR
T9299 24668-24672 Protein denotes exsA
T9300 24713-24717 Protein denotes exsA
T9301 24804-24809 Protein denotes IL-1β
T9302 24897-24907 Protein denotes host IL-1β
T9303 24727-24736 Regulation denotes regulator
T9304 24810-24819 Localization denotes secretion
T9305 24790-24799 Positive_regulation denotes important
T9306 25272-25277 Protein denotes NF-κB
T9307 25278-25290 Protein denotes Inflammasome
T10375 26112-26117 Protein denotes ΔF508
T10376 26122-26128 Protein denotes W1282X
T10377 26097-26107 Gene_expression denotes expressing
T10378 26097-26107 Gene_expression denotes expressing
T10379 26253-26257 Protein denotes CFTR
T10380 26365-26378 Protein denotes CFTR genotype
T10381 26380-26384 Protein denotes THP1
T10382 26423-26453 Protein denotes embryonic alkaline phosphatase
T10383 26455-26459 Protein denotes SEAP
T10384 26483-26488 Protein denotes NF-κB
T10385 26493-26509 Protein denotes AP-1 (Invivogen)
T10386 26404-26411 Gene_expression denotes express
T10387 26404-26411 Gene_expression denotes express
T10388 26414-26422 Localization denotes secreted
T10389 26414-26422 Localization denotes secreted
T10390 26470-26479 Positive_regulation denotes inducible
T10391 26470-26479 Positive_regulation denotes inducible
T10392 26834-26855 Protein denotes supplement bullet kit
T10393 26857-26860 Protein denotes EGF
T10394 26904-26915 Protein denotes transferrin
T10395 26917-26931 Protein denotes bovine insulin
T10396 27184-27188 Protein denotes THP1
T10397 27227-27231 Protein denotes RPMI
T10398 27307-27314 Protein denotes SEAP NF
T10399 27315-27317 Protein denotes κB
T10400 27318-27331 Protein denotes AP-1 reporter
T10401 27292-27302 Gene_expression denotes expressing
T10402 27292-27302 Gene_expression denotes expressing
T10403 27292-27302 Gene_expression denotes expressing
T10404 27528-27530 Protein denotes S9
T10405 27535-27540 Protein denotes IB3-1
T10406 27582-27602 Protein denotes bovine serum albumin
T10407 27616-27627 Protein denotes fibronectin
T10408 27741-27757 Protein denotes collagen type IV
T10860 29100-29113 Protein denotes complete RPMI
T11369 29862-29866 Protein denotes PAO1
T11370 29938-29942 Protein denotes PAO1
T11371 30211-30216 Protein denotes NF-κB
T11372 30391-30416 Protein denotes CFTR inhibitor CFTRinh172
T11373 30418-30431 Protein denotes Sigma Aldrich
T11374 30503-30512 Protein denotes caspase-1
T11748 30926-30955 Protein denotes Halt protease and phosphatase
T11749 31301-31334 Protein denotes IκBα (Cell Signaling), or β-actin
T11750 31596-31609 Protein denotes serum albumin
T12128 31797-31806 Protein denotes caspase-1
T12129 32001-32005 Protein denotes PAO1
T12130 32284-32288 Protein denotes PAO1
T12131 32302-32311 Protein denotes Caspase-1
T12132 32446-32455 Protein denotes caspase-1
T12133 32433-32438 Binding denotes binds
T12134 32544-32548 Protein denotes CD14
T12135 32661-32665 Protein denotes CD14
T12136 32666-32674 Gene_expression denotes positive
T12293 32752-32757 Protein denotes NF-κB
T12294 32758-32762 Protein denotes AP-1
T12295 32956-32976 Protein denotes alkaline phosphatase
T12296 33006-33011 Protein denotes NF-κB
T12297 33012-33016 Protein denotes AP-1
T12602 33587-33591 Protein denotes PAO1
T12603 33698-33702 Protein denotes PAO1
T12604 33876-33880 Protein denotes PAO1
T12605 33959-33963 Protein denotes PAO1
T12782 7068-7082 Protein denotes THP-1 reporter
T13106 7338-7343 Protein denotes IL-1β
T13107 7344-7354 Gene_expression denotes production
T13108 7566-7571 Protein denotes IL-1β
T13109 7753-7758 Protein denotes IL-1β
T13110 7763-7767 Protein denotes IL-8
T13111 7768-7778 Gene_expression denotes production
T13112 7768-7778 Gene_expression denotes production
T13113 7849-7854 Protein denotes IL-1β
T13403 8764-8773 Protein denotes caspase-1
T13404 8753-8763 Positive_regulation denotes upregulate
T13405 9401-9405 Protein denotes IL-6
T13623 10006-10015 Protein denotes caspase-1
T13624 9993-10002 Positive_regulation denotes increases
T13902 11952-11957 Protein denotes IL-1β
T13903 11923-11930 Gene_expression denotes produce
T13904 11931-11940 Positive_regulation denotes increased
T13905 12122-12137 Protein denotes aeruginosa PAO1
T13906 12146-12150 Protein denotes exsA
T13907 12138-12145 Negative_regulation denotes lacking
T13908 12138-12145 Negative_regulation denotes lacking
T13909 12235-12239 Protein denotes exsA
T13910 12227-12234 Negative_regulation denotes lacking
T14485 14091-14096 Protein denotes NF-κB
T14486 14134-14139 Protein denotes IL-1β
T14487 14097-14107 Positive_regulation denotes activation
T14488 14140-14150 Gene_expression denotes production
T14489 14124-14130 Negative_regulation denotes degree
T14490 14108-14119 Positive_regulation denotes potentiates
T14491 14436-14438 Protein denotes κB
T14492 14439-14443 Protein denotes AP-1
T14493 14649-14653 Protein denotes PAO1
T14494 14662-14666 Protein denotes PAO1
T14495 14717-14719 Protein denotes κB
T14496 14720-14724 Protein denotes AP-1
T14497 14753-14758 Protein denotes IL-1β
T14498 14759-14768 Localization denotes secretion
T14499 14759-14768 Localization denotes secretion
T14500 14924-14928 Protein denotes PAO1
T14501 15020-15025 Protein denotes IL-1β
T14651 16178-16182 Protein denotes PAO1
T15001 17144-17148 Protein denotes CFTR
T15002 17176-17181 Protein denotes IL-1β
T15003 17130-17140 Negative_regulation denotes Disruption
T15004 17182-17192 Gene_expression denotes production
T15005 17167-17175 Positive_regulation denotes increase
T15006 17353-17357 Protein denotes PAO1
T15007 17533-17543 Protein denotes CFTRinh172
T15008 17591-17596 Protein denotes IL-1β
T15009 17597-17607 Gene_expression denotes production
T15010 17706-17710 Protein denotes PAO1
T15011 17770-17772 Protein denotes κB
T15012 17773-17777 Protein denotes AP-1
R667 T802 T804 themeOf Interleukin-1 beta,mediator
R668 T803 T805 themeOf IL-1β,mediator
R669 T806 T807 themeOf IL-1β,Secretion
R670 T809 T811 themeOf IL-1β,produce
R671 T810 T812 themeOf caspase-1,upregulate
R672 T813 T815 themeOf IL-1β,produced
R673 T814 T816 themeOf caspase-1,upregulated
R674 T816 T817 themeOf upregulated,amounts
R675 T818 T820 themeOf caspase-1,activation
R676 T819 T821 themeOf IL-1β,production
R677 T821 T822 themeOf production,activation
R678 T823 T825 themeOf IL-1β,production
R679 T825 T826 themeOf production,dependent
R680 T825 T827 themeOf production,enhanced
R681 T828 T831 themeOf CFTR,inhibition
R682 T829 T832 themeOf IL-1β,production
R683 T830 T833 themeOf IL-8,production
R684 T832 T834 themeOf production,alter
R685 T833 T835 themeOf production,alter
R686 T837 T839 themeOf IL-1β,secretion
R687 T838 T840 themeOf CF pathogen Pseudomonas aeruginosa,secretion
R688 T841 T844 themeOf IL-1β,production
R689 T842 T846 themeOf NF-κB,increase
R690 T843 T847 themeOf CFTR,loss
R691 T844 T845 themeOf production,due
R692 T844 T848 themeOf production,increased
R2173 T2515 T2516 themeOf CFTR,lacking
R2181 T2517 T2519 themeOf Interleukin-1 beta,mediator
R2186 T2518 T2520 themeOf IL-1β,mediator
R2189 T2521 T2523 themeOf IL-6,induction
R2190 T2522 T2524 themeOf IL-8,induction
R2191 T2526 T2527 themeOf IL-1β,produced
R2192 T2528 T2530 themeOf IL-1β,secretion
R2193 T2529 T2531 themeOf pro-IL-18,secretion
R2194 T2533 T2536 themeOf IL-1β,increase
R2195 T2534 T2537 themeOf IL-6,increase
R2196 T2535 T2538 themeOf IL-8,increase
R2197 T2541 T2542 themeOf IL-1β,expression
R2198 T2542 T2543 themeOf expression,upregulation
R2199 T2545 T2546 themeOf IL-1β,increased
R2200 T2549 T2552 themeOf IL-1β,expression
R2201 T2550 T2553 themeOf CFTR,loss
R2202 T2551 T2554 themeOf NF-κB,activation
R2203 T2552 T2555 themeOf expression,increases
R2204 T2553 T2556 causeOf loss,increases
R2205 T2554 T2556 themeOf activation,increases
R2206 T2557 T2560 themeOf IL-1β,secretion
R2207 T2559 T2561 themeOf NLRP3,activation
R2208 T2560 T2562 themeOf secretion,increases
R2651 T3057 T3058 themeOf IL-1β,produce
R2652 T3059 T3062 themeOf IL-1β,production
R2653 T3062 T3063 themeOf production,increased
R2654 T3064 T3066 themeOf IL-1β,increased
R2655 T3065 T3067 themeOf IL-1β,production
R2656 T3067 T3068 themeOf production,increase
R2657 T3070 T3072 themeOf IL-8,producing
R2658 T3071 T3073 themeOf Fig,producing
R2927 T3378 T3379 themeOf caspase-1,upregulate
R2928 T3381 T3391 causeOf caspase-1,role
R2929 T3382 T3388 themeOf NF-κB,activation
R2930 T3385 T3389 themeOf caspase-1,inhibition
R2931 T3386 T3393 causeOf NF-κB,dependent
R2932 T3387 T3390 themeOf IL-6,production
R2933 T3388 T3391 themeOf activation,role
R2934 T3390 T3392 themeOf production,altered
R2935 T3390 T3393 themeOf production,dependent
R2936 T3395 T3397 themeOf IL-6,secretion
R2937 T3397 T3398 themeOf secretion,decrease
R3097 T3573 T3574 themeOf caspase-1,increases
R3098 T3577 T3581 themeOf caspase-1,activation
R3099 T3581 T3582 themeOf activation,increase
R3727 T4279 T4280 themeOf IL-1β,produce
R3728 T4279 T4281 themeOf IL-1β,increased
R3729 T4282 T4283 themeOf NF-κB,increased
R3730 T4282 T4284 themeOf NF-κB,secretion
R3731 T4284 T4285 themeOf secretion,increased
R3732 T4287 T4289 themeOf NLRC4,activate
R3733 T4288 T4290 themeOf NLRP3,activate
R3734 T4291 T4295 themeOf IL-1β,produce
R3735 T4291 T4299 themeOf IL-1β,increased
R3736 T4292 T4296 themeOf Fig. 5a,produce
R3737 T4292 T4300 themeOf Fig. 5a,increased
R3738 T4293 T4297 themeOf IL-8,produce
R3739 T4293 T4301 themeOf IL-8,increased
R3740 T4294 T4298 themeOf Fig. 5b,produce
R3741 T4294 T4302 themeOf Fig. 5b,increased
R3742 T4303 T4304 themeOf IL-1β,produced
R3743 T4305 T4309 themeOf exsA,lacks
R3744 T4306 T4310 themeOf PAO1ΔexsA,lacks
R3745 T4307 T4311 themeOf IL-1β,produced
R3746 T4312 T4313 themeOf IL-1β,production
R3747 T4313 T4314 themeOf production,result
R3748 T4315 T4318 themeOf IL-1β,production
R3749 T4316 T4319 themeOf IL-8,production
R3750 T4318 T4320 themeOf production,increased
R3751 T4319 T4321 themeOf production,increased
R4444 T5117 T5120 themeOf NF-κB,activation
R4445 T5118 T5121 themeOf IL-1β,responses
R4446 T5119 T5122 themeOf IL-8,responses
R4447 T5123 T5129 causeOf PAO1,induced
R4448 T5123 T5137 causeOf PAO1,induced
R4449 T5123 T5138 causeOf PAO1,induced
R4450 T5124 T5129 themeOf IL-1β,induced
R4451 T5124 T5130 themeOf IL-1β,production
R4452 T5124 T5133 causeOf IL-1β,dependence
R4453 T5124 T5134 causeOf IL-1β,dependence
R4454 T5124 T5135 causeOf IL-1β,dependence
R4455 T5124 T5136 causeOf IL-1β,dependence
R4456 T5125 T5131 themeOf IL-8,production
R4457 T5126 T5132 themeOf NF-κB,activation
R4458 T5129 T5133 themeOf induced,dependence
R4459 T5130 T5134 themeOf production,dependence
R4460 T5130 T5137 themeOf production,induced
R4461 T5131 T5135 themeOf production,dependence
R4462 T5131 T5138 themeOf production,induced
R4463 T5132 T5136 themeOf activation,dependence
R4464 T5140 T5149 themeOf NF-κB,activation
R4465 T5141 T5150 themeOf AP-1,activation
R4466 T5142 T5147 themeOf Fig. 6a,produced
R4467 T5144 T5148 themeOf Fig. 6b,produced
R4468 T5145 T5151 themeOf IL-1β,amounts
R4469 T5146 T5152 themeOf Fig. 6c,secreted
R4470 T5154 T5159 themeOf NF-κB,increase
R4471 T5156 T5160 themeOf Fig. 6a,increase
R4472 T5157 T5161 themeOf IL-8 secretion,increase
R4473 T5158 T5162 themeOf Fig. 6b,increase
R4474 T5163 T5167 themeOf IL-1β,production
R4475 T5167 T5168 themeOf production,augmented
R4476 T5169 T5173 themeOf NF-κB,activation
R4477 T5170 T5174 themeOf IL-1β,secretion
R4478 T5172 T5175 themeOf IL-1β,production
R4479 T5175 T5176 themeOf production,augmenting
R4480 T5178 T5184 themeOf NF-κB,inhibition
R4481 T5179 T5185 themeOf IκBα,phosphorylation
R4482 T5182 T5186 themeOf IL-1β,production
R4483 T5184 T5190 causeOf inhibition,reduced
R4484 T5185 T5187 themeOf phosphorylation,inhibitor
R4485 T5186 T5188 themeOf production,activation
R4486 T5186 T5189 themeOf production,subsequent
R4487 T5188 T5190 themeOf activation,reduced
R4488 T5191 T5198 causeOf NF-κB,modulator
R4489 T5192 T5195 themeOf IL-1β,production
R4490 T5193 T5196 themeOf NF-κB,activation
R4491 T5194 T5197 themeOf IL-1β,production
R4492 T5195 T5198 themeOf production,modulator
R4493 T5196 T5199 themeOf activation,increased
R4494 T5197 T5200 themeOf production,augments
R4729 T5457 T5459 themeOf NF-κB,inhibition
R4730 T5458 T5460 themeOf NLRP3 inflammasome,inhibit
R4731 T5459 T5460 causeOf inhibition,inhibit
R4732 T5461 T5466 themeOf NF-κB,inhibitor
R4733 T5462 T5467 themeOf CFTR,inhibition
R4734 T5463 T5467 causeOf CFTRinh172,inhibition
R4735 T5468 T5471 themeOf IκBα,degradation
R4736 T5469 T5472 themeOf PAO1,stimulation
R4737 T5471 T5473 themeOf degradation,inhibit
R4738 T5472 T5474 themeOf stimulation,inhibit
R5050 T5835 T5837 themeOf IL-1β,production
R5051 T5836 T5838 themeOf IL-8,production
R5042 T5823 T5825 themeOf CFTR,Disruption
R5043 T5824 T5826 themeOf IL-1β,production
R5044 T5825 T5827 causeOf Disruption,increase
R5045 T5826 T5827 themeOf production,increase
R5046 T5829 T5832 themeOf CFTR,dysfunction
R5047 T5830 T5833 themeOf IL-1β,production
R5048 T5832 T5834 causeOf dysfunction,alters
R5049 T5833 T5834 themeOf production,alters
R5052 T5837 T5839 themeOf production,alter
R5053 T5838 T5840 themeOf production,alter
R5054 T5841 T5844 themeOf IL-1β,production
R7997 T9156 T9157 themeOf IL-1β,increased
R8000 T9156 T9158 themeOf IL-1β,production
R8001 T9159 T9162 themeOf IL-1β,produce
R8002 T9160 T9163 themeOf caspase-1,activation
R8003 T9163 T9164 themeOf activation,increase
R8008 T9165 T9166 themeOf IL-1β,source
R8010 T9167 T9168 themeOf CFTR,expressed
R8014 T9169 T9172 themeOf IL-1β,production
R8018 T9174 T9175 themeOf IL-1β,overproduction
R8021 T9175 T9176 themeOf overproduction,overproduction
R8024 T9177 T9180 themeOf IL-1β,secrete
R8027 T9177 T9181 themeOf IL-1β,increased
R8029 T9178 T9182 themeOf IL-1β,production
R8032 T9179 T9183 themeOf CF PBMCs,production
R8035 T9182 T9184 themeOf production,increased
R8041 T9183 T9185 themeOf production,increased
R8044 T9186 T9188 themeOf IL-1β,production
R8049 T9187 T9189 themeOf CF PBMCs,production
R8051 T9188 T9190 themeOf production,increased
R8052 T9189 T9191 themeOf production,increased
R8054 T9192 T9194 themeOf IL-1β,production
R8057 T9193 T9196 themeOf NF-κB,activation
R8061 T9194 T9195 themeOf production,increased
R8064 T9197 T9199 themeOf CFTR,deficient
R8068 T9198 T9200 themeOf NF-κB,increased
R8071 T9258 T9265 causeOf NF-κB,role
R8072 T9259 T9263 themeOf IL-1β,regulation
R8073 T9260 T9264 causeOf deletion,increases
R8074 T9262 T9264 themeOf secretion,increases
R8075 T9263 T9265 themeOf regulation,role
R8076 T9268 T9269 themeOf NF-κB,dependent
R8077 T9271 T9273 themeOf IL-1β,inhibited
R8078 T9201 T9202 themeOf IL-1β,secretion
R8079 T9271 T9274 themeOf IL-1β,secretion
R8080 T9272 T9275 themeOf IL-8,secretion
R8081 T9203 T9204 themeOf IL-1β,secretion
R8082 T9204 T9205 themeOf secretion,increased
R8083 T9274 T9276 themeOf secretion,inhibited
R8084 T9206 T9209 themeOf PBMCs,expressed
R8085 T9207 T9210 themeOf basal NF-κB,expressed
R8086 T9207 T9211 themeOf basal NF-κB,increased
R8087 T9208 T9212 themeOf IL-1β,secretion
R8088 T9275 T9277 themeOf secretion,inhibited
R8089 T9212 T9213 themeOf secretion,increase
R8090 T9280 T9281 themeOf NF-κB,activation
R8091 T9214 T9215 themeOf IL-1β,increase
R8092 T9216 T9217 themeOf IL-1β,production
R8093 T9219 T9221 themeOf IL-1β,produce
R8094 T9220 T9222 themeOf NF-κB,activation
R8095 T9221 T9223 themeOf produce,reduced
R8096 T9282 T9288 themeOf IL-1β,response
R8097 T9283 T9289 themeOf NF-κB,activation
R8098 T9222 T9224 themeOf activation,induce
R8099 T9284 T9290 themeOf AP-1,activation
R8100 T9225 T9230 themeOf ΔF508,expressing
R8101 T9226 T9231 themeOf CFTR,expressing
R8102 T9285 T9291 themeOf Fig. 6a,activation
R8103 T9227 T9232 themeOf IL-1β,produced
R8104 T9228 T9233 themeOf CFTR,expressing
R8105 T9286 T9292 themeOf IL-8 secretion,activation
R8106 T9288 T9293 themeOf response,induce
R8107 T9234 T9235 themeOf CFTR,expression
R8108 T9289 T9294 themeOf activation,generated
R8109 T9236 T9239 themeOf IL-1β,produce
R8110 T9290 T9295 themeOf activation,generated
R8111 T9238 T9240 themeOf IL-1β,production
R8112 T9291 T9296 themeOf activation,generated
R8113 T9240 T9241 themeOf production,effects
R8114 T9242 T9243 themeOf IL-1β,production
R8115 T9292 T9297 themeOf activation,generated
R8116 T9244 T9246 themeOf IL-1β,produce
R8117 T9300 T9303 themeOf exsA,regulator
R8118 T9245 T9247 themeOf caspase-1,produce
R8119 T9248 T9250 themeOf NF-κB,activation
R8120 T9249 T9251 themeOf IL-1β,secretion
R8121 T9252 T9253 themeOf NF-κB,activation
R8122 T9253 T9254 themeOf activation,role
R8123 T9255 T9260 themeOf IKKβ,deletion
R8124 T9256 T9261 themeOf NF-κB,activation
R8125 T9301 T9304 themeOf IL-1β,secretion
R8126 T9257 T9262 themeOf IL-1β,secretion
R8127 T9304 T9305 themeOf secretion,important
R9119 T10375 T10377 themeOf ΔF508,expressing
R9120 T10376 T10378 themeOf W1282X,expressing
R9121 T10382 T10386 themeOf embryonic alkaline phosphatase,express
R9122 T10382 T10388 themeOf embryonic alkaline phosphatase,secreted
R9123 T10382 T10390 themeOf embryonic alkaline phosphatase,inducible
R9124 T10382 T10391 themeOf embryonic alkaline phosphatase,inducible
R9125 T10383 T10387 themeOf SEAP,express
R9126 T10383 T10389 themeOf SEAP,secreted
R9127 T10384 T10390 causeOf NF-κB,inducible
R9128 T10385 T10391 causeOf AP-1 (Invivogen),inducible
R9129 T10398 T10401 themeOf SEAP NF,expressing
R9130 T10399 T10402 themeOf κB,expressing
R9131 T10400 T10403 themeOf AP-1 reporter,expressing
R10742 T12132 T12133 themeOf caspase-1,binds
R10743 T12135 T12136 themeOf CD14,positive
R11615 T13106 T13107 themeOf IL-1β,production
R11616 T13109 T13111 themeOf IL-1β,production
R11617 T13110 T13112 themeOf IL-8,production
R11887 T13403 T13404 themeOf caspase-1,upregulate
R12095 T13623 T13624 themeOf caspase-1,increases
R12339 T13902 T13903 themeOf IL-1β,produce
R12340 T13902 T13904 themeOf IL-1β,increased
R12341 T13905 T13907 themeOf aeruginosa PAO1,lacking
R12342 T13906 T13908 themeOf exsA,lacking
R12343 T13909 T13910 themeOf exsA,lacking
R12872 T14485 T14487 themeOf NF-κB,activation
R12873 T14486 T14488 themeOf IL-1β,production
R12874 T14487 T14490 causeOf activation,potentiates
R12875 T14488 T14489 themeOf production,degree
R12876 T14489 T14490 themeOf degree,potentiates
R12877 T14495 T14498 themeOf κB,secretion
R12878 T14497 T14499 themeOf IL-1β,secretion
R13332 T15001 T15003 themeOf CFTR,Disruption
R13333 T15002 T15004 themeOf IL-1β,production
R13334 T15003 T15005 causeOf Disruption,increase
R13335 T15004 T15005 themeOf production,increase
R13336 T15008 T15009 themeOf IL-1β,production

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T767 13-21 Positive_regulation denotes Mediated
T768 22-27 Protein denotes IL-1β
T769 28-38 Gene_expression denotes Production
T770 288-306 Protein denotes Interleukin-1 beta
T771 308-313 Protein denotes IL-1β
T772 347-356 Localization denotes Secretion
T773 380-385 Protein denotes IL-1β
T774 386-394 Regulation denotes involves
T775 471-476 Protein denotes IL-1β
T776 560-568 Positive_regulation denotes mediated true
T777 569-574 Protein denotes IL-1β
T778 575-585 Gene_expression denotes production
T779 707-714 Gene_expression denotes produce true
T780 746-756 Positive_regulation denotes stimulated
T781 757-762 Protein denotes IL-1β
T782 985-993 Gene_expression denotes produced
T783 1011-1016 Protein denotes IL-1β
T784 1061-1065 Positive_regulation denotes upon
T785 1181-1186 Protein denotes IL-1β
T786 1187-1197 Gene_expression denotes production
T787 1203-1213 Positive_regulation denotes stimulated
T788 1249-1254 Protein denotes IL-1β
T789 1442-1447 Regulation denotes alter true
T790 1448-1453 Protein denotes IL-1β
T791 1462-1472 Gene_expression denotes production
T792 1473-1487 Positive_regulation denotes in response to
T793 1587-1594 Positive_regulation denotes induced
T794 1612-1617 Protein denotes IL-1β
T795 1664-1673 Positive_regulation denotes preserved
T796 1702-1707 Protein denotes IL-1β
T797 1708-1717 Localization denotes secretion
T798 1832-1841 Positive_regulation denotes increased
T799 1841-1847 Protein denotes IL-1β
T800 1848-1858 Gene_expression denotes production
T801 1877-1880 Positive_regulation denotes due true
T2487 3138-3156 Protein denotes Interleukin-1 beta
T2488 3158-3163 Protein denotes IL-1β
T2489 3453-3457 Protein denotes IL-6
T2490 3568-3573 Protein denotes IL-1β
T2491 4377-4385 Positive_regulation denotes triggers
T2492 4377-4385 Positive_regulation denotes triggers
T2493 4389-4397 Positive_regulation denotes increase
T2494 4389-4397 Positive_regulation denotes increase
T2495 4411-4416 Protein denotes IL-1β
T2496 4418-4422 Protein denotes IL-6
T2497 4565-4574 Regulation denotes important
T2498 4659-4664 Protein denotes IL-1β
T2499 4665-4675 Gene_expression denotes expression
T2500 4724-4729 Protein denotes IL-1β
T2501 4793-4798 Protein denotes IL-1β
T2502 4803-4812 Positive_regulation denotes increased
T2503 4990-4994 Protein denotes IL1B
T2504 5149-5158 Positive_regulation denotes increases
T2505 5149-5158 Positive_regulation denotes increases
T2506 5162-5167 Protein denotes IL-1β
T2507 5168-5178 Gene_expression denotes expression
T2508 5182-5191 Localization denotes secretion
T2509 5444-5453 Positive_regulation denotes increases
T2510 5454-5463 Localization denotes secretion
T2511 5467-5472 Protein denotes IL-1β
T2512 5676-5681 Protein denotes IL-1β
T3044 5788-5795 Gene_expression denotes produce
T3045 5819-5824 Protein denotes IL-1β
T3046 5825-5839 Positive_regulation denotes in response to true
T3047 6229-6239 Positive_regulation denotes contribute true
T3048 6247-6256 Positive_regulation denotes increased
T3049 6257-6262 Protein denotes IL-1β
T3050 6263-6273 Gene_expression denotes production
T3051 6447-6452 Protein denotes IL-1β
T3052 6506-6515 Positive_regulation denotes increased true
T3053 6585-6593 Positive_regulation denotes increase
T3054 6597-6602 Protein denotes IL-1β
T3055 6603-6613 Gene_expression denotes production
T3056 6805-6810 Protein denotes IL-1β
T3370 8474-8481 Regulation denotes altered true
T3371 8488-8497 Regulation denotes dependent
T3372 8498-8502 Protein denotes IL-6
T3373 8503-8513 Gene_expression denotes production
T3374 8514-8528 Positive_regulation denotes in response to
T3375 8618-8626 Negative_regulation denotes decrease true
T3376 8627-8631 Protein denotes IL-6
T3377 8632-8641 Localization denotes secretion
T3571 9606-9610 Protein denotes CD14
T4256 10456-10463 Gene_expression denotes produce
T4257 10464-10473 Positive_regulation denotes increased true
T4258 10485-10490 Protein denotes IL-1β
T4259 10933-10940 Gene_expression denotes produce
T4260 10941-10950 Positive_regulation denotes increased true
T4261 10962-10967 Protein denotes IL-1β
T4262 11066-11074 Negative_regulation denotes decrease
T4263 11102-11107 Protein denotes IL-1β
T4264 11108-11116 Gene_expression denotes produced
T4265 11129-11143 Positive_regulation denotes in response to
T4266 11232-11236 Protein denotes exsA
T4267 11289-11297 Gene_expression denotes produced
T4268 11309-11313 Negative_regulation denotes less
T4269 11314-11319 Protein denotes IL-1β
T4270 11429-11435 Positive_regulation denotes result true
T4271 11443-11448 Protein denotes IL-1β
T4272 11449-11459 Gene_expression denotes production true
T4273 11585-11594 Positive_regulation denotes increased true
T4274 11585-11594 Positive_regulation denotes increased true
T4275 11595-11605 Gene_expression denotes production
T4276 11595-11605 Gene_expression denotes production
T4277 11609-11614 Protein denotes IL-1β
T4278 11618-11622 Protein denotes IL-8
T5087 12400-12408 Positive_regulation denotes required
T5088 12413-12418 Protein denotes IL-1β
T5089 12428-12437 Regulation denotes responses
T5090 12473-12483 Regulation denotes dependence
T5091 12492-12499 Positive_regulation denotes induced
T5092 12500-12505 Protein denotes IL-1β
T5093 12515-12525 Gene_expression denotes production
T5094 12848-12853 Protein denotes IL-1β
T5095 12859-12867 Localization denotes secreted true
T5096 13055-13060 Protein denotes IL-1β
T5097 13061-13071 Gene_expression denotes production
T5098 13084-13093 Positive_regulation denotes augmented
T5099 13250-13260 Positive_regulation denotes sufficient true
T5100 13273-13278 Protein denotes IL-1β
T5101 13279-13288 Localization denotes secretion
T5102 13346-13351 Protein denotes IL-1β
T5103 13519-13528 Negative_regulation denotes inhibitor
T5104 13532-13536 Protein denotes IκBα
T5105 13537-13552 Phosphorylation denotes phosphorylation
T5106 13574-13581 Negative_regulation denotes reduced
T5107 13643-13653 Gene_expression denotes production
T5108 13685-13690 Protein denotes IL-1β
T5109 13711-13725 Positive_regulation denotes in response to
T5110 13926-13935 Regulation denotes modulator
T5111 13939-13944 Protein denotes IL-1β
T5112 13945-13955 Gene_expression denotes production
T5113 13995-14003 Positive_regulation denotes augments
T5114 14017-14025 Positive_regulation denotes mediated
T5115 14026-14036 Gene_expression denotes production
T5116 14040-14045 Protein denotes IL-1β
T5454 15800-15807 Negative_regulation denotes inhibit
T5455 15808-15812 Protein denotes IκBα
T5456 15813-15824 Protein_catabolism denotes degradation
T5812 16334-16342 Positive_regulation denotes increase true
T5813 16343-16348 Protein denotes IL-1β
T5814 16349-16359 Gene_expression denotes production
T5815 16546-16552 Regulation denotes alters true
T5816 16553-16558 Protein denotes IL-1β
T5817 16559-16569 Gene_expression denotes production
T5818 16760-16765 Regulation denotes alter true
T5819 16766-16771 Protein denotes IL-1β
T5820 16780-16790 Gene_expression denotes production
T5821 16850-16855 Protein denotes IL-1β
T5822 16856-16866 Gene_expression denotes production
T9066 17830-17835 Protein denotes IL-1β
T9067 17840-17849 Positive_regulation denotes increased
T9068 17926-17936 Gene_expression denotes production
T9069 17955-17963 Regulation denotes targeted true
T9070 18234-18241 Gene_expression denotes produce true
T9071 18265-18270 Protein denotes IL-1β
T9072 18520-18525 Protein denotes IL-1β
T9073 18867-18877 Gene_expression denotes production
T9074 18881-18886 Protein denotes IL-1β
T9075 18975-18980 Protein denotes IL-1β
T9076 19247-19252 Protein denotes IL-1β
T9077 19351-19365 Gene_expression denotes overproduction
T9078 19369-19374 Protein denotes IL-1β
T9079 19531-19538 Localization denotes secrete
T9080 19539-19548 Positive_regulation denotes increased true
T9081 19560-19565 Protein denotes IL-1β
T9082 19581-19586 Protein denotes IL-1β
T9083 19587-19597 Gene_expression denotes production
T9084 19618-19627 Positive_regulation denotes increased true
T9085 19765-19774 Positive_regulation denotes increased
T9086 19775-19780 Protein denotes IL-1β
T9087 19781-19791 Gene_expression denotes production
T9088 19935-19940 Protein denotes IL-1β
T9089 19941-19951 Gene_expression denotes production
T9090 19960-19969 Positive_regulation denotes increased true
T9091 20320-20338 Positive_regulation denotes This amplification
T9092 20342-20347 Protein denotes IL-1β
T9093 20348-20357 Localization denotes secretion
T9094 20507-20516 Positive_regulation denotes increased
T9095 20517-20522 Protein denotes IL-1β
T9096 20523-20532 Localization denotes secretion
T9097 20684-20692 Positive_regulation denotes increase
T9098 20696-20701 Protein denotes IL-1β
T9099 20702-20711 Localization denotes secretion
T9100 20719-20726 Regulation denotes absence true
T9101 20755-20763 Positive_regulation denotes increase true
T9102 20767-20772 Protein denotes IL-1β
T9103 20846-20856 Gene_expression denotes production
T9104 20860-20865 Protein denotes IL-1β
T9105 21038-21045 Negative_regulation denotes reduced
T9106 21058-21065 Gene_expression denotes produce
T9107 21066-21071 Protein denotes IL-1β
T9108 21551-21559 Positive_regulation denotes mediated
T9109 21560-21565 Protein denotes IL-1β
T9110 21566-21576 Gene_expression denotes production
T9111 21793-21800 Gene_expression denotes produce
T9112 21818-21823 Protein denotes IL-1β
T9113 21865-21872 Regulation denotes effects true
T9114 21893-21898 Protein denotes IL-1β
T9115 21899-21909 Gene_expression denotes production
T9116 21946-21956 Positive_regulation denotes contribute true
T9117 21960-21965 Protein denotes IL-1β
T9118 21966-21976 Gene_expression denotes production
T9119 22093-22100 Gene_expression denotes produce
T9120 22108-22113 Protein denotes IL-1β
T9121 22132-22143 Regulation denotes independent true true
T9122 22165-22169 Regulation denotes role
T9123 22221-22226 Protein denotes IL-1β
T9124 22227-22236 Localization denotes secretion
T9125 22478-22487 Positive_regulation denotes increases
T9126 22488-22493 Protein denotes IL-1β
T9127 22494-22503 Localization denotes secretion
T9128 22556-22565 Regulation denotes dual role
T9129 22579-22589 Regulation denotes regulation
T9130 22593-22598 Protein denotes IL-1β
T9131 23029-23038 Negative_regulation denotes inhibited
T9132 23044-23049 Protein denotes IL-1β
T9133 23059-23068 Localization denotes secretion
T9134 23097-23102 Protein denotes IL-1β
T9135 23112-23121 Regulation denotes responses
T9136 23141-23150 Regulation denotes dependent
T9137 23237-23243 Positive_regulation denotes induce true
T9138 23253-23258 Protein denotes IL-1β
T9139 23259-23267 Regulation denotes response
T9140 24694-24702 Negative_regulation denotes deletion
T9141 24713-24717 Protein denotes exsA
T9142 24804-24809 Protein denotes IL-1β
T9143 24842-24851 Regulation denotes depending
T9144 24842-24851 Regulation denotes depending
T9145 24902-24907 Protein denotes IL-1β
T9146 24908-24916 Regulation denotes response
T9147 24924-24926 Positive_regulation denotes up true
T9148 24930-24943 Negative_regulation denotes downregulated true
T9149 25103-25111 Positive_regulation denotes mediated
T9150 25112-25117 Protein denotes IL-1β
T9151 25118-25128 Gene_expression denotes production
T9152 25142-25156 Positive_regulation denotes in response to
T9153 25231-25240 Positive_regulation denotes increased
T9154 25241-25246 Protein denotes IL-1β
T9155 25260-25268 Regulation denotes response true true
T10372 26904-26915 Protein denotes transferrin
T10373 26924-26931 Protein denotes insulin
T10374 27589-27602 Protein denotes serum albumin
T11746 31301-31305 Protein denotes IκBα
T11747 31327-31334 Protein denotes β-actin
T12127 32661-32665 Protein denotes CD14
T12292 32956-32976 Protein denotes alkaline phosphatase
T13098 7324-7334 Positive_regulation denotes contribute true
T13099 7338-7343 Protein denotes IL-1β
T13100 7344-7354 Gene_expression denotes production
T13101 7355-7369 Positive_regulation denotes in response to
T13102 7566-7571 Protein denotes IL-1β
T13103 7753-7758 Protein denotes IL-1β
T13104 7768-7778 Gene_expression denotes production
T13105 7849-7854 Protein denotes IL-1β
T13402 9401-9405 Protein denotes IL-6
T13894 11923-11930 Gene_expression denotes produce
T13895 11931-11940 Positive_regulation denotes increased true
T13896 11952-11957 Protein denotes IL-1β
T13897 12138-12145 Negative_regulation denotes lacking
T13898 12146-12150 Protein denotes exsA
T13899 12227-12234 Negative_regulation denotes lacking
T13900 12235-12239 Protein denotes exsA
T13901 12359-12364 Protein denotes IL-1β
T14473 14108-14119 Positive_regulation denotes potentiates
T14474 14108-14119 Positive_regulation denotes potentiates
T14475 14134-14139 Protein denotes IL-1β
T14476 14140-14150 Gene_expression denotes production
T14477 14155-14164 Localization denotes secretion
T14478 14165-14169 Positive_regulation denotes upon
T14479 14165-14169 Positive_regulation denotes upon
T14480 14472-14477 Protein denotes IL-1β
T14481 14478-14487 Localization denotes secretion
T14482 14753-14758 Protein denotes IL-1β
T14483 14759-14768 Localization denotes secretion true
T14484 15020-15025 Protein denotes IL-1β
T14992 17167-17175 Positive_regulation denotes increase true
T14993 17176-17181 Protein denotes IL-1β
T14994 17182-17192 Gene_expression denotes production
T14995 17373-17378 Protein denotes IL-1β
T14996 17392-17402 Gene_expression denotes production true
T14997 17591-17596 Protein denotes IL-1β
T14998 17597-17607 Gene_expression denotes production true
T14999 17732-17737 Protein denotes IL-1β
T15000 17738-17747 Localization denotes secretion true
R645 T768 T769 themeOf IL-1β,Production
R646 T769 T767 themeOf Production,Mediated
R647 T771 T770 equivalentTo IL-1β,Interleukin-1 beta
R648 T772 T774 themeOf Secretion,involves
R649 T773 T772 themeOf IL-1β,Secretion
R650 T777 T778 themeOf IL-1β,production
R651 T778 T776 themeOf production,mediated
R652 T779 T780 themeOf produce,stimulated
R653 T781 T779 themeOf IL-1β,produce
R654 T782 T784 themeOf produced,upon
R655 T783 T782 themeOf IL-1β,produced
R656 T785 T786 themeOf IL-1β,production
R657 T786 T787 themeOf production,stimulated
R658 T790 T791 themeOf IL-1β,production
R659 T791 T792 themeOf production,in response to
R660 T792 T789 themeOf in response to,alter
R661 T794 T793 themeOf IL-1β,induced
R662 T796 T797 themeOf IL-1β,secretion
R663 T797 T795 themeOf secretion,preserved
R664 T798 T801 themeOf increased,due
R665 T799 T800 themeOf IL-1β,production
R666 T800 T798 themeOf production,increased
R2171 T2488 T2487 equivalentTo IL-1β,Interleukin-1 beta
R2174 T2493 T2491 themeOf increase,triggers
R2175 T2494 T2492 themeOf increase,triggers
R2176 T2495 T2494 themeOf IL-1β,increase
R2177 T2496 T2493 themeOf IL-6,increase
R2178 T2498 T2499 themeOf IL-1β,expression
R2179 T2499 T2497 themeOf expression,important
R2180 T2501 T2502 themeOf IL-1β,increased
R2182 T2506 T2507 themeOf IL-1β,expression
R2183 T2506 T2508 themeOf IL-1β,secretion
R2184 T2507 T2505 themeOf expression,increases
R2185 T2508 T2504 themeOf secretion,increases
R2187 T2510 T2509 themeOf secretion,increases
R2188 T2511 T2510 themeOf IL-1β,secretion
R2643 T3044 T3046 themeOf produce,in response to
R2644 T3045 T3044 themeOf IL-1β,produce
R2645 T3048 T3047 themeOf increased,contribute
R2646 T3049 T3050 themeOf IL-1β,production
R2647 T3050 T3048 themeOf production,increased
R2648 T3051 T3052 themeOf IL-1β,increased
R2649 T3054 T3055 themeOf IL-1β,production
R2650 T3055 T3053 themeOf production,increase
R2921 T3371 T3374 themeOf dependent,in response to
R2922 T3372 T3373 themeOf IL-6,production
R2923 T3373 T3371 themeOf production,dependent
R2924 T3374 T3370 themeOf in response to,altered
R2925 T3376 T3377 themeOf IL-6,secretion
R2926 T3377 T3375 themeOf secretion,decrease
R3712 T4256 T4257 themeOf produce,increased
R3713 T4258 T4256 themeOf IL-1β,produce
R3714 T4259 T4260 themeOf produce,increased
R3715 T4261 T4259 themeOf IL-1β,produce
R3716 T4262 T4265 themeOf decrease,in response to
R3717 T4263 T4264 themeOf IL-1β,produced
R3718 T4264 T4262 themeOf produced,decrease
R3719 T4267 T4268 themeOf produced,less
R3720 T4269 T4267 themeOf IL-1β,produced
R3721 T4271 T4272 themeOf IL-1β,production
R3722 T4272 T4270 themeOf production,result
R3723 T4275 T4273 themeOf production,increased
R3724 T4276 T4274 themeOf production,increased
R3725 T4277 T4275 themeOf IL-1β,production
R3726 T4278 T4276 themeOf IL-8,production
R4423 T5088 T5089 themeOf IL-1β,responses
R4424 T5089 T5087 themeOf responses,required
R4425 T5091 T5090 themeOf induced,dependence
R4426 T5092 T5093 themeOf IL-1β,production
R4427 T5093 T5091 themeOf production,induced
R4428 T5094 T5095 themeOf IL-1β,secreted
R4429 T5096 T5097 themeOf IL-1β,production
R4430 T5097 T5098 themeOf production,augmented
R4431 T5100 T5101 themeOf IL-1β,secretion
R4432 T5101 T5099 themeOf secretion,sufficient
R4433 T5103 T5106 causeOf inhibitor,reduced
R4434 T5104 T5105 themeOf IκBα,phosphorylation
R4435 T5105 T5103 themeOf phosphorylation,inhibitor
R4436 T5107 T5109 themeOf production,in response to
R4437 T5108 T5107 themeOf IL-1β,production
R4438 T5109 T5106 themeOf in response to,reduced
R4439 T5111 T5112 themeOf IL-1β,production
R4440 T5112 T5110 themeOf production,modulator
R4441 T5114 T5113 themeOf mediated,augments
R4442 T5115 T5114 themeOf production,mediated
R4443 T5116 T5115 themeOf IL-1β,production
R4727 T5455 T5456 themeOf IκBα,degradation
R4728 T5456 T5454 themeOf degradation,inhibit
R5035 T5813 T5814 themeOf IL-1β,production
R5036 T5814 T5812 themeOf production,increase
R5037 T5816 T5817 themeOf IL-1β,production
R5038 T5817 T5815 themeOf production,alters
R5039 T5819 T5820 themeOf IL-1β,production
R5040 T5820 T5818 themeOf production,alter
R5041 T5821 T5822 themeOf IL-1β,production
R7960 T9066 T9067 themeOf IL-1β,increased
R7964 T9066 T9068 themeOf IL-1β,production
R7966 T9068 T9069 themeOf production,targeted
R7970 T9071 T9070 themeOf IL-1β,produce
R7974 T9074 T9073 themeOf IL-1β,production
R7978 T9078 T9077 themeOf IL-1β,overproduction
R7982 T9079 T9080 themeOf secrete,increased
R7986 T9081 T9079 themeOf IL-1β,secrete
R7992 T9082 T9083 themeOf IL-1β,production
R7996 T9083 T9084 themeOf production,increased
R8004 T9086 T9087 themeOf IL-1β,production
R8005 T9087 T9085 themeOf production,increased
R8006 T9088 T9089 themeOf IL-1β,production
R8007 T9089 T9090 themeOf production,increased
R8009 T9092 T9093 themeOf IL-1β,secretion
R8011 T9093 T9091 themeOf secretion,This amplification
R8012 T9095 T9096 themeOf IL-1β,secretion
R8013 T9096 T9094 themeOf secretion,increased
R8015 T9097 T9100 themeOf increase,absence
R8016 T9098 T9099 themeOf IL-1β,secretion
R8017 T9099 T9097 themeOf secretion,increase
R8019 T9102 T9101 themeOf IL-1β,increase
R8020 T9104 T9103 themeOf IL-1β,production
R8022 T9106 T9105 themeOf produce,reduced
R8023 T9107 T9106 themeOf IL-1β,produce
R8025 T9109 T9110 themeOf IL-1β,production
R8026 T9110 T9108 themeOf production,mediated
R8028 T9112 T9111 themeOf IL-1β,produce
R8030 T9114 T9115 themeOf IL-1β,production
R8031 T9115 T9113 themeOf production,effects
R8033 T9117 T9118 themeOf IL-1β,production
R8034 T9118 T9116 themeOf production,contribute
R8036 T9119 T9121 themeOf produce,independent
R8037 T9120 T9119 themeOf IL-1β,produce
R8038 T9123 T9124 themeOf IL-1β,secretion
R8039 T9124 T9122 themeOf secretion,role
R8040 T9126 T9127 themeOf IL-1β,secretion
R8042 T9127 T9125 themeOf secretion,increases
R8043 T9129 T9128 themeOf regulation,dual role
R8045 T9130 T9129 themeOf IL-1β,regulation
R8046 T9132 T9133 themeOf IL-1β,secretion
R8047 T9133 T9131 themeOf secretion,inhibited
R8048 T9134 T9135 themeOf IL-1β,responses
R8050 T9135 T9136 themeOf responses,dependent
R8053 T9138 T9139 themeOf IL-1β,response
R8055 T9139 T9137 themeOf response,induce
R8056 T9141 T9140 themeOf exsA,deletion
R8058 T9145 T9146 themeOf IL-1β,response
R8059 T9146 T9147 themeOf response,up
R8060 T9146 T9148 themeOf response,downregulated
R8062 T9147 T9143 themeOf up,depending
R8063 T9148 T9144 themeOf downregulated,depending
R8065 T9149 T9152 themeOf mediated,in response to
R8066 T9150 T9151 themeOf IL-1β,production
R8067 T9151 T9149 themeOf production,mediated
R8069 T9154 T9155 themeOf IL-1β,response
R8070 T9155 T9153 themeOf response,increased
R11611 T13099 T13100 themeOf IL-1β,production
R11612 T13100 T13101 themeOf production,in response to
R11613 T13101 T13098 themeOf in response to,contribute
R11614 T13103 T13104 themeOf IL-1β,production
R12335 T13894 T13895 themeOf produce,increased
R12336 T13896 T13894 themeOf IL-1β,produce
R12337 T13898 T13897 themeOf exsA,lacking
R12338 T13900 T13899 themeOf exsA,lacking
R12864 T14473 T14479 themeOf potentiates,upon
R12865 T14474 T14478 themeOf potentiates,upon
R12866 T14475 T14476 themeOf IL-1β,production
R12867 T14475 T14477 themeOf IL-1β,secretion
R12868 T14476 T14474 themeOf production,potentiates
R12869 T14477 T14473 themeOf secretion,potentiates
R12870 T14480 T14481 themeOf IL-1β,secretion
R12871 T14482 T14483 themeOf IL-1β,secretion
R13327 T14993 T14994 themeOf IL-1β,production
R13328 T14994 T14992 themeOf production,increase
R13329 T14995 T14996 themeOf IL-1β,production
R13330 T14997 T14998 themeOf IL-1β,production
R13331 T14999 T15000 themeOf IL-1β,secretion

ICD10

Id Subject Object Predicate Lexical cue
T694 266-281 http://purl.bioontology.org/ontology/ICD10/E84 denotes cystic fibrosis
T695 266-281 http://purl.bioontology.org/ontology/ICD10/E84.9 denotes cystic fibrosis
T2419 2047-2062 http://purl.bioontology.org/ontology/ICD10/E84 denotes cystic fibrosis
T2420 2047-2062 http://purl.bioontology.org/ontology/ICD10/E84.9 denotes cystic fibrosis
T2421 3340-3345 http://purl.bioontology.org/ontology/ICD10/R50.9 denotes fever

GO-CC

Id Subject Object Predicate Lexical cue
T688 613-617 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T689 682-686 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T690 789-794 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T691 929-934 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T692 1414-1419 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T693 1529-1534 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2422 2063-2076 http://purl.obolibrary.org/obo/GO_0016021 denotes transmembrane
T2423 2063-2076 http://purl.obolibrary.org/obo/GO_0044214 denotes transmembrane
T2424 2558-2562 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T2425 2558-2568 http://purl.obolibrary.org/obo/GO_0043657 denotes host cells
T2426 2563-2568 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2427 2641-2646 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2428 2825-2830 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2429 3388-3393 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2430 3489-3493 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2431 5425-5429 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2432 3593-3600 http://purl.obolibrary.org/obo/GO_0005829 denotes cytosol
T2433 3721-3738 http://purl.obolibrary.org/obo/GO_0043234 denotes protein complexes
T2434 5576-5589 http://purl.obolibrary.org/obo/GO_0005576 denotes extracellular
T3011 5775-5780 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3012 6053-6058 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3013 6223-6228 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3014 6648-6653 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3015 6679-6684 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3016 6725-6730 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3349 7954-7959 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3350 8112-8117 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3351 8663-8668 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4207 11123-11128 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5017 12558-12563 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5018 12681-12686 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5019 12916-12921 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5020 13106-13111 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5021 13157-13162 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5786 16379-16384 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5787 16577-16582 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5788 16935-16940 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5789 17079-17084 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8865 18042-18047 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8866 18221-18226 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8867 18410-18415 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8868 18443-18448 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8869 18785-18790 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8870 19099-19104 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8871 19227-19232 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8872 19519-19524 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8873 20982-20987 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8874 21931-21936 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8875 22846-22851 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8876 22963-22968 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8877 23837-23842 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8878 25031-25036 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8879 25060-25065 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8880 19177-19181 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T8881 19222-19226 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T8882 19267-19271 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T8883 24897-24901 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T8884 19222-19232 http://purl.obolibrary.org/obo/GO_0043657 denotes host cells
T8885 22813-22826 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T10347 25931-25935 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T10348 25994-25999 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10349 26063-26068 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10350 26199-26204 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10351 26215-26220 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10352 26308-26313 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10353 26391-26396 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10354 26615-26620 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10355 26775-26780 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10356 27195-27200 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10357 27286-27291 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10358 27455-27460 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10359 27846-27851 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10360 27876-27881 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10361 27902-27907 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10362 28068-28073 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10363 28096-28101 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10858 29191-29195 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T10859 29358-29363 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11364 29482-29486 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T11365 29548-29553 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11366 29641-29646 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11367 29791-29796 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11368 29906-29911 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11736 30821-30826 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11737 31172-31181 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T11738 31421-31431 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T11739 31421-31431 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T12116 31837-31842 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12117 31881-31886 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12118 32134-32139 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12119 32154-32159 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12120 32645-32650 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12121 32744-32749 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12122 32549-32559 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T12123 32549-32559 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T12289 32811-32816 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12781 6912-6916 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T13078 7297-7302 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13079 7498-7503 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13080 7710-7714 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T13394 8731-8736 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13395 8833-8838 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13396 9066-9071 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13397 9169-9174 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13398 9182-9187 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13399 9330-9334 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T13622 10364-10369 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14444 14210-14215 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14445 14561-14566 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14650 16023-16028 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14971 17636-17641 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T696 1457-1461 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T2413 2137-2148 http://purl.obolibrary.org/obo/GO_0022831 denotes ion channel
T2414 3453-3457 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T2415 4418-4422 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T2416 3462-3466 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T2417 4428-4432 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T2418 3921-3926 http://purl.obolibrary.org/obo/GO_0045515 denotes IL-18
T3017 6842-6846 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T3352 8498-8502 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T3353 8627-8631 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T4208 10981-10985 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T4209 11618-11622 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T5022 12423-12427 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T5023 12510-12514 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T5024 12797-12801 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T5025 13001-13005 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T5026 13657-13661 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T5784 16775-16779 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T5785 16978-16982 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T8861 22666-22670 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T8862 23054-23058 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T8863 23107-23111 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T8864 23371-23375 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T10364 26442-26453 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T10365 26857-26860 http://purl.obolibrary.org/obo/GO_0005154 denotes EGF
T11740 30944-30955 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T11741 31421-31431 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T12124 32549-32559 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T12288 32965-32976 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T13077 7802-7806 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T13081 7763-7767 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T13393 9401-9405 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T13877 12373-12377 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T14446 14458-14462 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T14447 14739-14743 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T14448 15034-15038 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T14972 17387-17391 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T14973 17753-17757 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8

GO-BP

Id Subject Object Predicate Lexical cue
T680 128-140 http://purl.obolibrary.org/obo/GO_0006954 denotes Inflammation
T681 288-306 http://purl.obolibrary.org/obo/GO_0050720 denotes Interleukin-1 beta
T682 288-306 http://purl.obolibrary.org/obo/GO_0050702 denotes Interleukin-1 beta
T683 300-310 http://purl.obolibrary.org/obo/GO_0032611 denotes 1 beta (IL
T684 347-356 http://purl.obolibrary.org/obo/GO_0046903 denotes Secretion
T685 1708-1717 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T686 1457-1472 http://purl.obolibrary.org/obo/GO_0032677 denotes IL-8 production
T687 1457-1472 http://purl.obolibrary.org/obo/GO_0032637 denotes IL-8 production
T2392 2137-2148 http://purl.obolibrary.org/obo/GO_0022831 denotes ion channel
T2393 2318-2321 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T2394 2443-2448 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T2395 2467-2479 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T2396 2787-2808 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T2397 2706-2723 http://purl.obolibrary.org/obo/GO_0034097 denotes cytokine response
T2398 3138-3156 http://purl.obolibrary.org/obo/GO_0050720 denotes Interleukin-1 beta
T2399 3138-3156 http://purl.obolibrary.org/obo/GO_0050702 denotes Interleukin-1 beta
T2400 3150-3160 http://purl.obolibrary.org/obo/GO_0032611 denotes 1 beta (IL
T2401 3270-3282 http://purl.obolibrary.org/obo/GO_0009405 denotes pathogenesis
T2402 4737-4749 http://purl.obolibrary.org/obo/GO_0009405 denotes pathogenesis
T2403 3326-3345 http://purl.obolibrary.org/obo/GO_0001660 denotes generation of fever
T2404 3549-3558 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T2405 4539-4548 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T2406 3549-3566 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling cascade
T2407 3809-3817 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T2408 3854-3875 http://purl.obolibrary.org/obo/GO_0006919 denotes activation of caspase
T2409 3968-3977 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2410 5182-5191 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2411 5454-5463 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2412 5278-5294 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T3010 6161-6178 http://purl.obolibrary.org/obo/GO_0034097 denotes cytokine response
T3341 8002-8022 http://purl.obolibrary.org/obo/GO_0014823 denotes activity in response
T3342 8352-8371 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T3343 8405-8414 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3344 8498-8513 http://purl.obolibrary.org/obo/GO_0032635 denotes IL-6 production
T3345 8498-8513 http://purl.obolibrary.org/obo/GO_0032675 denotes IL-6 production
T3346 8627-8641 http://purl.obolibrary.org/obo/GO_1900165 denotes IL-6 secretion
T3347 8627-8641 http://purl.obolibrary.org/obo/GO_0072604 denotes IL-6 secretion
T3348 8632-8641 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T4203 10628-10646 http://purl.obolibrary.org/obo/GO_0050663 denotes cytokine secretion
T4204 10637-10646 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T4205 11278-11287 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T4206 11132-11147 http://purl.obolibrary.org/obo/GO_0032496 denotes response to LPS
T5001 12423-12437 http://purl.obolibrary.org/obo/GO_0098758 denotes IL-8 responses
T5002 12423-12437 http://purl.obolibrary.org/obo/GO_0098759 denotes IL-8 responses
T5003 12510-12525 http://purl.obolibrary.org/obo/GO_0032677 denotes IL-8 production
T5004 13643-13661 http://purl.obolibrary.org/obo/GO_0032677 denotes production of IL-8
T5005 12510-12525 http://purl.obolibrary.org/obo/GO_0032637 denotes IL-8 production
T5006 13643-13661 http://purl.obolibrary.org/obo/GO_0032637 denotes production of IL-8
T5007 12797-12811 http://purl.obolibrary.org/obo/GO_0072606 denotes IL-8 secretion
T5008 13001-13015 http://purl.obolibrary.org/obo/GO_0072606 denotes IL-8 secretion
T5009 12797-12811 http://purl.obolibrary.org/obo/GO_2000482 denotes IL-8 secretion
T5010 13001-13015 http://purl.obolibrary.org/obo/GO_2000482 denotes IL-8 secretion
T5011 12802-12811 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5012 13006-13015 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5013 13279-13288 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5014 13367-13376 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5015 13537-13552 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T5016 13975-13994 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T5445 15452-15467 http://purl.obolibrary.org/obo/GO_0032088 denotes NF-κB inhibitor
T5446 15452-15467 http://purl.obolibrary.org/obo/GO_0007252 denotes NF-κB inhibitor
T5447 15813-15824 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T5782 16775-16790 http://purl.obolibrary.org/obo/GO_0032677 denotes IL-8 production
T5783 16775-16790 http://purl.obolibrary.org/obo/GO_0032637 denotes IL-8 production
T8826 17885-17893 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T8827 18548-18556 http://purl.obolibrary.org/obo/GO_0048286 denotes alveolar
T8828 18947-18955 http://purl.obolibrary.org/obo/GO_0048286 denotes alveolar
T8829 19000-19021 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T8830 23521-23542 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T8831 25247-25268 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T8832 19807-19822 http://purl.obolibrary.org/obo/GO_0032496 denotes response to LPS
T8833 20223-20242 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T8834 20309-20318 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8835 20348-20357 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8836 20523-20532 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8837 20702-20711 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8838 22227-22236 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8839 22494-22503 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8840 23059-23068 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8841 23376-23385 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8842 24481-24490 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8843 24810-24819 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8844 24882-24891 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T8845 21416-21444 http://purl.obolibrary.org/obo/GO_0030224 denotes differentiation of monocytes
T8846 22579-22589 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T8847 22988-23003 http://purl.obolibrary.org/obo/GO_0032088 denotes NF-κB inhibitor
T8848 22988-23003 http://purl.obolibrary.org/obo/GO_0007252 denotes NF-κB inhibitor
T8849 23054-23068 http://purl.obolibrary.org/obo/GO_0072606 denotes IL-8 secretion
T8850 23371-23385 http://purl.obolibrary.org/obo/GO_0072606 denotes IL-8 secretion
T8851 23054-23068 http://purl.obolibrary.org/obo/GO_2000482 denotes IL-8 secretion
T8852 23371-23385 http://purl.obolibrary.org/obo/GO_2000482 denotes IL-8 secretion
T8853 23107-23121 http://purl.obolibrary.org/obo/GO_0098758 denotes IL-8 responses
T8854 23107-23121 http://purl.obolibrary.org/obo/GO_0098759 denotes IL-8 responses
T8855 23651-23666 http://purl.obolibrary.org/obo/GO_0006412 denotes translatability
T8856 24112-24125 http://purl.obolibrary.org/obo/GO_0045087 denotes innate immune
T8857 24642-24651 http://purl.obolibrary.org/obo/GO_0009405 denotes virulence
T8858 24642-24651 http://purl.obolibrary.org/obo/GO_0016032 denotes virulence
T8859 24745-24758 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T8860 25145-25160 http://purl.obolibrary.org/obo/GO_0033198 denotes response to ATP
T9441 25791-25794 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T10345 26442-26453 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T10346 27913-27945 http://purl.obolibrary.org/obo/GO_0030225 denotes differentiated into a macrophage
T10856 28704-28726 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory responses
T10857 28799-28802 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T11735 30944-30955 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T12287 32965-32976 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T13072 7763-7778 http://purl.obolibrary.org/obo/GO_0032677 denotes IL-8 production
T13073 7763-7778 http://purl.obolibrary.org/obo/GO_0032637 denotes IL-8 production
T13074 7802-7816 http://purl.obolibrary.org/obo/GO_0072606 denotes IL-8 secretion
T13075 7802-7816 http://purl.obolibrary.org/obo/GO_2000482 denotes IL-8 secretion
T13076 7807-7816 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T13392 8774-8794 http://purl.obolibrary.org/obo/GO_0014823 denotes activity in response
T13876 12263-12272 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T14441 14155-14164 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T14442 14478-14487 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T14443 14759-14768 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T14967 17387-17402 http://purl.obolibrary.org/obo/GO_0032677 denotes IL-8 production
T14968 17387-17402 http://purl.obolibrary.org/obo/GO_0032637 denotes IL-8 production
T14969 17470-17501 http://purl.obolibrary.org/obo/GO_0030225 denotes differentiated into macrophages
T14970 17738-17747 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion

UBERON-AE

Id Subject Object Predicate Lexical cue
T36 911-916 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T901 2402-2406 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T902 3286-3290 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T903 4769-4773 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T5939 19458-19462 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T5940 23670-23674 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T5941 23904-23908 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T5942 25423-25427 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T5943 19458-19473 http://purl.obolibrary.org/obo/UBERON_0008946 denotes lung parenchyma
T5944 19463-19473 http://purl.obolibrary.org/obo/UBERON_0000353 denotes parenchyma
T5945 21223-21234 http://purl.obolibrary.org/obo/UBERON_0002371 denotes bone marrow
T5946 23831-23836 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T9308 25575-25580 http://purl.obolibrary.org/obo/UBERON_0000178 denotes Blood
T9448 26704-26709 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T9449 26803-26808 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T9450 27589-27594 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T10409 28301-28306 http://purl.obolibrary.org/obo/UBERON_0001089 denotes sweat
T10410 28444-28449 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T10411 28858-28863 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T10412 28896-28901 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T10413 29462-29467 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T11377 31596-31601 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T11753 32057-32062 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T378 0-21 NNP denotes Inflammasome-Mediated
T379 22-27 NNP denotes IL-1β
T380 28-38 NN denotes Production
T381 39-41 IN denotes in
T382 42-48 NNP denotes Humans
T383 49-53 IN denotes with
T384 54-60 NNP denotes Cystic
T385 61-69 NNP denotes Fibrosis
T386 117-127 NNP denotes Background
T387 128-140 NNP denotes Inflammation
T388 141-144 CC denotes and
T389 145-154 NN denotes infection
T390 155-158 VBP denotes are
T391 159-164 JJ denotes major
T392 165-177 NNS denotes determinants
T393 178-180 IN denotes of
T394 181-188 NN denotes disease
T395 189-197 NN denotes severity
T396 198-201 CC denotes and
T397 202-214 RB denotes consequently
T398 214-215 , denotes ,
T399 216-219 DT denotes the
T400 220-227 NN denotes quality
T401 228-230 IN denotes of
T402 231-235 NN denotes life
T403 236-239 CC denotes and
T404 240-247 NN denotes outcome
T405 248-251 IN denotes for
T406 252-260 NNS denotes patients
T407 261-265 IN denotes with
T408 266-272 JJ denotes cystic
T409 273-281 NN denotes fibrosis
T410 282-283 -LRB- denotes (
T411 283-285 NNP denotes CF
T412 285-286 -RRB- denotes )
T413 286-287 . denotes .
T414 288-301 NN denotes Interleukin-1
T415 302-306 NN denotes beta
T416 307-308 -LRB- denotes (
T417 308-313 NN denotes IL-1β
T418 313-314 -RRB- denotes )
T419 315-317 VBZ denotes is
T420 318-319 DT denotes a
T421 320-323 JJ denotes key
T422 324-336 JJ denotes inflammatory
T423 337-345 NN denotes mediator
T424 345-346 . denotes .
T425 347-356 NNP denotes Secretion
T426 357-359 IN denotes of
T427 360-372 RB denotes biologically
T428 373-379 JJ denotes active
T429 380-385 JJ denotes IL-1β
T430 386-394 VBZ denotes involves
T431 395-416 JJ denotes inflammasome-mediated
T432 417-427 NN denotes processing
T433 427-428 . denotes .
T434 429-435 NNP denotes Little
T435 436-438 VBZ denotes is
T436 439-444 VBN denotes known
T437 445-450 IN denotes about
T438 451-454 DT denotes the
T439 455-467 NN denotes contribution
T440 468-470 IN denotes of
T441 471-476 NN denotes IL-1β
T442 477-480 CC denotes and
T443 481-484 DT denotes the
T444 485-498 NNS denotes inflammasomes
T445 499-501 IN denotes in
T446 502-504 NNP denotes CF
T447 505-517 JJ denotes inflammatory
T448 518-525 NN denotes disease
T449 525-526 . denotes .
T450 527-531 DT denotes This
T451 532-537 NN denotes study
T452 538-546 VBZ denotes examines
T453 547-568 JJ denotes inflammasome-mediated
T454 569-574 JJ denotes IL-1β
T455 575-585 NN denotes production
T456 586-588 IN denotes in
T457 589-591 NNP denotes CF
T458 592-601 JJ denotes bronchial
T459 602-612 JJ denotes epithelial
T460 613-617 NN denotes cell
T461 618-623 NNS denotes lines
T462 624-627 CC denotes and
T463 628-633 JJ denotes human
T464 634-642 NNS denotes patients
T465 643-647 IN denotes with
T466 648-651 NNP denotes CF.
T467 653-660 NNS denotes Results
T468 661-670 NNP denotes Bronchial
T469 671-681 JJ denotes epithelial
T470 682-686 NN denotes cell
T471 687-692 NNS denotes lines
T472 693-697 VBD denotes were
T473 698-703 VBN denotes found
T474 704-706 TO denotes to
T475 707-714 VB denotes produce
T476 715-725 JJ denotes negligible
T477 726-733 NNS denotes amounts
T478 734-736 IN denotes of
T479 737-742 NN denotes basal
T480 743-745 CC denotes or
T481 746-756 VBN denotes stimulated
T482 757-762 NN denotes IL-1β
T483 763-771 VBN denotes compared
T484 772-774 TO denotes to
T485 775-788 JJ denotes hematopoeitic
T486 789-794 NNS denotes cells
T487 795-798 CC denotes and
T488 799-803 PRP denotes they
T489 804-807 VBD denotes did
T490 808-811 RB denotes not
T491 812-825 RB denotes significantly
T492 826-836 VB denotes upregulate
T493 837-846 JJ denotes caspase-1
T494 847-855 NN denotes activity
T495 856-860 IN denotes upon
T496 861-873 JJ denotes inflammasome
T497 874-885 NN denotes stimulation
T498 885-886 . denotes .
T499 887-889 IN denotes In
T500 890-898 NN denotes contrast
T501 898-899 , denotes ,
T502 900-910 JJ denotes peripheral
T503 911-916 NN denotes blood
T504 917-928 NN denotes mononuclear
T505 929-934 NNS denotes cells
T506 935-936 -LRB- denotes (
T507 936-941 NNS denotes PBMCs
T508 941-942 -RRB- denotes )
T509 943-947 IN denotes from
T510 948-952 DT denotes both
T511 953-955 NNP denotes CF
T512 956-959 CC denotes and
T513 960-967 JJ denotes healthy
T514 968-975 NN denotes control
T515 976-984 NNS denotes subjects
T516 985-993 VBD denotes produced
T517 994-999 JJ denotes large
T518 1000-1007 NNS denotes amounts
T519 1008-1010 IN denotes of
T520 1011-1016 NN denotes IL-1β
T521 1017-1020 CC denotes and
T522 1021-1029 RB denotes strongly
T523 1030-1041 VBD denotes upregulated
T524 1042-1051 JJ denotes caspase-1
T525 1052-1060 NN denotes activity
T526 1061-1065 IN denotes upon
T527 1066-1078 JJ denotes inflammasome
T528 1079-1090 NN denotes stimulation
T529 1090-1091 . denotes .
T530 1092-1097 NNS denotes PBMCs
T531 1098-1102 IN denotes from
T532 1103-1105 NNP denotes CF
T533 1106-1114 NNS denotes patients
T534 1115-1118 CC denotes and
T535 1119-1127 NNS denotes controls
T536 1128-1137 VBD denotes displayed
T537 1138-1145 JJ denotes similar
T538 1146-1152 NNS denotes levels
T539 1153-1155 IN denotes of
T540 1156-1165 JJ denotes caspase-1
T541 1166-1176 NN denotes activation
T542 1177-1180 CC denotes and
T543 1181-1186 JJ denotes IL-1β
T544 1187-1197 NN denotes production
T545 1198-1202 WRB denotes when
T546 1203-1213 VBN denotes stimulated
T547 1214-1218 IN denotes with
T548 1219-1231 JJ denotes inflammasome
T549 1232-1242 NNS denotes activators
T550 1242-1243 . denotes .
T551 1244-1248 DT denotes This
T552 1249-1254 JJ denotes IL-1β
T553 1255-1265 NN denotes production
T554 1266-1269 VBD denotes was
T555 1270-1279 JJ denotes dependent
T556 1280-1282 IN denotes on
T557 1283-1288 JJ denotes NF-κB
T558 1289-1297 NN denotes activity
T559 1298-1301 CC denotes and
T560 1302-1307 MD denotes could
T561 1308-1310 VB denotes be
T562 1311-1319 VBN denotes enhanced
T563 1320-1322 IN denotes by
T564 1323-1330 VBG denotes priming
T565 1331-1335 IN denotes with
T566 1336-1339 NNP denotes LPS
T567 1339-1340 . denotes .
T568 1341-1348 RB denotes Finally
T569 1348-1349 , denotes ,
T570 1350-1358 NN denotes chemical
T571 1359-1369 NN denotes inhibition
T572 1370-1372 IN denotes of
T573 1373-1377 NNP denotes CFTR
T574 1378-1386 NN denotes activity
T575 1387-1389 IN denotes in
T576 1390-1397 NN denotes control
T577 1398-1403 NNS denotes PBMCs
T578 1404-1407 CC denotes and
T579 1408-1413 CD denotes THP-1
T580 1414-1419 NNS denotes cells
T581 1420-1423 VBD denotes did
T582 1424-1427 RB denotes not
T583 1428-1441 RB denotes significantly
T584 1442-1447 VB denotes alter
T585 1448-1453 JJ denotes IL-1β
T586 1454-1456 CC denotes or
T587 1457-1461 JJ denotes IL-8
T588 1462-1472 NN denotes production
T589 1473-1475 IN denotes in
T590 1476-1484 NN denotes response
T591 1485-1487 TO denotes to
T592 1488-1490 NNP denotes P.
T593 1491-1501 NN denotes aeruginosa
T594 1501-1502 . denotes .
T595 1504-1514 NNP denotes Conclusion
T596 1515-1528 NNP denotes Hematopoeitic
T597 1529-1534 NNS denotes cells
T598 1535-1541 VBP denotes appear
T599 1542-1544 TO denotes to
T600 1545-1547 VB denotes be
T601 1548-1551 DT denotes the
T602 1552-1563 JJ denotes predominant
T603 1564-1570 NN denotes source
T604 1571-1573 IN denotes of
T605 1574-1594 JJ denotes inflammasome-induced
T606 1595-1611 JJ denotes pro-inflammatory
T607 1612-1617 NN denotes IL-1β
T608 1618-1620 IN denotes in
T609 1621-1624 NNP denotes CF.
T610 1625-1630 NNP denotes PBMCs
T611 1631-1638 VBD denotes derived
T612 1639-1643 IN denotes from
T613 1644-1646 NNP denotes CF
T614 1647-1655 NNS denotes subjects
T615 1656-1663 VBP denotes display
T616 1664-1673 VBN denotes preserved
T617 1674-1686 JJ denotes inflammasome
T618 1687-1697 NN denotes activation
T619 1698-1701 CC denotes and
T620 1702-1707 JJ denotes IL-1β
T621 1708-1717 NN denotes secretion
T622 1718-1720 IN denotes in
T623 1721-1729 NN denotes response
T624 1730-1732 TO denotes to
T625 1733-1736 DT denotes the
T626 1737-1742 JJ denotes major
T627 1743-1745 NNP denotes CF
T628 1746-1754 NN denotes pathogen
T629 1755-1766 NNP denotes Pseudomonas
T630 1767-1777 NN denotes aeruginosa
T631 1777-1778 . denotes .
T632 1779-1786 RB denotes However
T633 1786-1787 , denotes ,
T634 1788-1791 PRP$ denotes our
T635 1792-1796 NNS denotes data
T636 1797-1799 VBP denotes do
T637 1800-1803 RB denotes not
T638 1804-1811 VB denotes support
T639 1812-1815 DT denotes the
T640 1816-1826 NN denotes hypothesis
T641 1827-1831 WDT denotes that
T642 1832-1841 VBD denotes increased
T643 1842-1847 JJ denotes IL-1β
T644 1848-1858 NN denotes production
T645 1859-1861 IN denotes in
T646 1862-1864 NNP denotes CF
T647 1865-1873 NNS denotes subjects
T648 1874-1876 VBZ denotes is
T649 1877-1880 JJ denotes due
T650 1881-1883 TO denotes to
T651 1884-1886 DT denotes an
T652 1887-1896 JJ denotes intrinsic
T653 1897-1905 NN denotes increase
T654 1906-1908 IN denotes in
T655 1909-1914 JJ denotes NF-κB
T656 1915-1923 NN denotes activity
T657 1924-1931 IN denotes through
T658 1932-1936 NN denotes loss
T659 1937-1939 IN denotes of
T660 1940-1944 NNP denotes CFTR
T661 1945-1953 NN denotes function
T662 1953-1954 . denotes .
T1659 1970-1976 JJ denotes Cystic
T1660 1977-1985 NN denotes fibrosis
T1661 1986-1988 VBZ denotes is
T1662 1989-1991 DT denotes an
T1663 1992-2001 JJ denotes autosomal
T1664 2002-2011 JJ denotes recessive
T1665 2012-2019 NN denotes disease
T1666 2020-2026 VBN denotes caused
T1667 2027-2029 IN denotes by
T1668 2030-2039 NNS denotes mutations
T1669 2040-2042 IN denotes in
T1670 2043-2046 DT denotes the
T1671 2047-2053 JJ denotes cystic
T1672 2054-2062 NN denotes fibrosis
T1673 2063-2076 NN denotes transmembrane
T1674 2077-2088 NN denotes conductance
T1675 2089-2098 NN denotes regulator
T1676 2099-2100 -LRB- denotes (
T1677 2100-2104 NNP denotes CFTR
T1678 2104-2105 -RRB- denotes )
T1679 2105-2106 , denotes ,
T1680 2107-2112 WDT denotes which
T1681 2113-2122 NNS denotes functions
T1682 2123-2125 IN denotes as
T1683 2126-2127 DT denotes a
T1684 2128-2136 NN denotes chloride
T1685 2137-2140 NN denotes ion
T1686 2141-2148 NN denotes channel
T1687 2148-2149 . denotes .
T1688 2150-2152 NN denotes CF
T1689 2153-2160 VBZ denotes remains
T1690 2161-2164 CD denotes one
T1691 2165-2167 IN denotes of
T1692 2168-2171 DT denotes the
T1693 2172-2176 RBS denotes most
T1694 2177-2183 JJ denotes common
T1695 2184-2190 JJ denotes lethal
T1696 2191-2198 JJ denotes genetic
T1697 2199-2207 NNS denotes diseases
T1698 2208-2210 IN denotes in
T1699 2211-2222 NNS denotes populations
T1700 2223-2225 IN denotes of
T1701 2226-2234 JJ denotes European
T1702 2235-2242 NN denotes descent
T1703 2243-2247 IN denotes with
T1704 2248-2251 DT denotes the
T1705 2252-2259 JJ denotes current
T1706 2260-2267 JJ denotes average
T1707 2268-2276 NN denotes lifespan
T1708 2277-2279 IN denotes of
T1709 2280-2282 NNP denotes CF
T1710 2283-2291 NNS denotes patients
T1711 2292-2305 RB denotes approximately
T1712 2306-2308 CD denotes 40
T1713 2309-2314 NNS denotes years
T1714 2315-2317 IN denotes of
T1715 2318-2321 NN denotes age
T1716 2322-2323 NN denotes [
T1717 2323-2324 CD denotes 1
T1718 2324-2325 NNP denotes ]
T1719 2325-2326 , denotes ,
T1720 2327-2328 NNP denotes [
T1721 2328-2329 CD denotes 2
T1722 2329-2330 NNP denotes ]
T1723 2330-2331 . denotes .
T1724 2332-2341 JJ denotes Recurrent
T1725 2342-2354 JJ denotes inflammatory
T1726 2355-2364 JJ denotes pulmonary
T1727 2365-2377 NN denotes exacerbation
T1728 2378-2380 VBZ denotes is
T1729 2381-2384 DT denotes the
T1730 2385-2392 JJ denotes primary
T1731 2393-2398 NN denotes cause
T1732 2399-2401 IN denotes of
T1733 2402-2406 NN denotes lung
T1734 2407-2414 NN denotes disease
T1735 2415-2426 NN denotes progression
T1736 2427-2430 CC denotes and
T1737 2431-2441 RB denotes ultimately
T1738 2441-2442 , denotes ,
T1739 2443-2448 NN denotes death
T1740 2449-2451 IN denotes in
T1741 2452-2455 NNP denotes CF.
T1742 2456-2466 NNP denotes Controlled
T1743 2467-2479 NN denotes inflammation
T1744 2480-2482 VBZ denotes is
T1745 2483-2492 JJ denotes important
T1746 2493-2496 IN denotes for
T1747 2497-2505 VBG denotes fighting
T1748 2506-2515 NN denotes infection
T1749 2515-2516 , denotes ,
T1750 2517-2520 CC denotes but
T1751 2521-2523 IN denotes in
T1752 2524-2530 JJ denotes excess
T1753 2530-2531 , denotes ,
T1754 2532-2534 PRP denotes it
T1755 2535-2542 VBZ denotes becomes
T1756 2543-2554 JJ denotes destructive
T1757 2555-2557 TO denotes to
T1758 2558-2562 VB denotes host
T1759 2563-2568 NNS denotes cells
T1760 2569-2572 CC denotes and
T1761 2573-2575 TO denotes to
T1762 2576-2579 DT denotes the
T1763 2580-2592 NN denotes architecture
T1764 2593-2595 IN denotes of
T1765 2596-2599 DT denotes the
T1766 2600-2605 NNS denotes lungs
T1767 2606-2607 VBP denotes [
T1768 2607-2608 CD denotes 2
T1769 2608-2609 NNP denotes ]
T1770 2609-2610 , denotes ,
T1771 2611-2612 NNP denotes [
T1772 2612-2613 CD denotes 3
T1773 2613-2614 NNP denotes ]
T1774 2614-2615 . denotes .
T1775 2616-2618 IN denotes In
T1776 2619-2621 NNP denotes CF
T1777 2621-2622 , denotes ,
T1778 2623-2629 NN denotes airway
T1779 2630-2640 JJ denotes epithelial
T1780 2641-2646 NNS denotes cells
T1781 2647-2651 VBP denotes have
T1782 2652-2656 VBN denotes been
T1783 2657-2662 VBN denotes shown
T1784 2663-2665 TO denotes to
T1785 2666-2673 VB denotes produce
T1786 2674-2676 DT denotes an
T1787 2677-2688 JJ denotes exaggerated
T1788 2689-2705 JJ denotes pro-inflammatory
T1789 2706-2714 NN denotes cytokine
T1790 2715-2723 NN denotes response
T1791 2724-2726 TO denotes to
T1792 2727-2738 NN denotes stimulation
T1793 2739-2740 NN denotes [
T1794 2740-2741 CD denotes 4
T1795 2741-2742 NNP denotes ]
T1796 2742-2743 , denotes ,
T1797 2744-2745 NNP denotes [
T1798 2745-2746 CD denotes 5
T1799 2746-2747 NNP denotes ]
T1800 2747-2748 . denotes .
T1801 2749-2751 PRP denotes It
T1802 2752-2754 VBZ denotes is
T1803 2755-2762 JJ denotes unclear
T1804 2763-2770 IN denotes whether
T1805 2771-2775 DT denotes this
T1806 2776-2786 VBN denotes heightened
T1807 2787-2799 JJ denotes inflammatory
T1808 2800-2808 NN denotes response
T1809 2809-2811 VBZ denotes is
T1810 2812-2821 JJ denotes intrinsic
T1811 2822-2824 TO denotes to
T1812 2825-2830 NNS denotes cells
T1813 2831-2838 VBG denotes lacking
T1814 2839-2843 NNP denotes CFTR
T1815 2844-2846 CC denotes or
T1816 2847-2854 IN denotes whether
T1817 2855-2857 PRP denotes it
T1818 2858-2860 VBZ denotes is
T1819 2861-2862 DT denotes a
T1820 2863-2869 NN denotes result
T1821 2870-2872 IN denotes of
T1822 2873-2880 JJ denotes chronic
T1823 2881-2894 JJ denotes polymicrobial
T1824 2895-2904 NN denotes infection
T1825 2905-2906 NN denotes [
T1826 2906-2907 CD denotes 6
T1827 2907-2908 NNP denotes ]
T1828 2908-2909 , denotes ,
T1829 2910-2911 NNP denotes [
T1830 2911-2912 CD denotes 7
T1831 2912-2913 NNP denotes ]
T1832 2913-2914 . denotes .
T1833 2915-2925 RB denotes Regardless
T1834 2926-2928 IN denotes of
T1835 2929-2933 DT denotes this
T1836 2934-2945 NN denotes controversy
T1837 2945-2946 , denotes ,
T1838 2947-2958 VBG denotes identifying
T1839 2959-2962 CC denotes and
T1840 2963-2972 VBG denotes targeting
T1841 2973-2981 JJ denotes relevant
T1842 2982-2994 JJ denotes inflammatory
T1843 2995-3004 NNS denotes mediators
T1844 3005-3007 VBZ denotes is
T1845 3008-3009 DT denotes a
T1846 3010-3018 JJ denotes critical
T1847 3019-3023 NN denotes step
T1848 3024-3026 IN denotes in
T1849 3027-3037 VBG denotes developing
T1850 3038-3042 RBR denotes more
T1851 3043-3051 JJ denotes specific
T1852 3052-3063 JJ denotes therapeutic
T1853 3064-3074 NNS denotes approaches
T1854 3075-3077 TO denotes to
T1855 3078-3085 VB denotes control
T1856 3086-3098 NN denotes inflammation
T1857 3099-3102 CC denotes and
T1858 3103-3110 VB denotes improve
T1859 3111-3117 NN denotes health
T1860 3118-3126 NNS denotes outcomes
T1861 3127-3129 IN denotes in
T1862 3130-3132 NNP denotes CF
T1863 3133-3134 NNP denotes [
T1864 3134-3135 CD denotes 8
T1865 3135-3136 NNP denotes ]
T1866 3136-3137 . denotes .
T1867 3138-3151 NN denotes Interleukin-1
T1868 3152-3156 NN denotes beta
T1869 3157-3158 -LRB- denotes (
T1870 3158-3163 NN denotes IL-1β
T1871 3163-3164 -RRB- denotes )
T1872 3165-3167 VBZ denotes is
T1873 3168-3169 DT denotes a
T1874 3170-3175 JJ denotes major
T1875 3176-3188 JJ denotes inflammatory
T1876 3189-3197 NN denotes mediator
T1877 3197-3198 . denotes .
T1878 3199-3202 PRP$ denotes Its
T1879 3203-3216 JJ denotes physiological
T1880 3217-3224 NNS denotes effects
T1881 3225-3228 VBP denotes are
T1882 3229-3236 JJ denotes diverse
T1883 3237-3240 CC denotes and
T1884 3241-3252 RB denotes potentially
T1885 3253-3262 JJ denotes important
T1886 3263-3265 TO denotes to
T1887 3266-3269 DT denotes the
T1888 3270-3282 NN denotes pathogenesis
T1889 3283-3285 IN denotes of
T1890 3286-3290 NN denotes lung
T1891 3291-3304 NNS denotes exacerbations
T1892 3305-3307 IN denotes in
T1893 3308-3310 NNP denotes CF
T1894 3310-3311 , denotes ,
T1895 3312-3321 VBG denotes including
T1896 3322-3325 DT denotes the
T1897 3326-3336 NN denotes generation
T1898 3337-3339 IN denotes of
T1899 3340-3345 NN denotes fever
T1900 3345-3346 , denotes ,
T1901 3347-3350 DT denotes the
T1902 3351-3362 NN denotes recruitment
T1903 3363-3365 IN denotes of
T1904 3366-3378 JJ denotes inflammatory
T1905 3379-3387 NN denotes effector
T1906 3388-3393 NNS denotes cells
T1907 3393-3394 , denotes ,
T1908 3395-3398 DT denotes the
T1909 3399-3408 NN denotes induction
T1910 3409-3411 IN denotes of
T1911 3412-3417 JJ denotes other
T1912 3418-3434 JJ denotes pro-inflammatory
T1913 3435-3444 NNS denotes cytokines
T1914 3445-3449 JJ denotes such
T1915 3450-3452 IN denotes as
T1916 3453-3457 NNP denotes IL-6
T1917 3458-3461 CC denotes and
T1918 3462-3466 NNP denotes IL-8
T1919 3466-3467 , denotes ,
T1920 3468-3471 CC denotes and
T1921 3472-3475 DT denotes the
T1922 3476-3483 VBG denotes shaping
T1923 3484-3486 IN denotes of
T1924 3487-3488 NNP denotes T
T1925 3489-3493 NN denotes cell
T1926 3494-3503 NNS denotes responses
T1927 3504-3505 VBP denotes [
T1928 3505-3506 CD denotes 9
T1929 3506-3507 NNP denotes ]
T1930 3507-3508 , denotes ,
T1931 3509-3510 NNP denotes [
T1932 3510-3512 CD denotes 10
T1933 3512-3513 NNP denotes ]
T1934 3513-3514 . denotes .
T1935 3515-3524 VBG denotes Following
T1936 3525-3535 NN denotes initiation
T1937 3536-3538 IN denotes of
T1938 3539-3542 DT denotes the
T1939 3543-3548 NNP denotes NF-κB
T1940 3549-3558 VBG denotes signaling
T1941 3559-3566 NN denotes cascade
T1942 3566-3567 , denotes ,
T1943 3568-3573 NN denotes IL-1β
T1944 3574-3576 VBZ denotes is
T1945 3577-3585 VBN denotes produced
T1946 3586-3588 IN denotes in
T1947 3589-3592 DT denotes the
T1948 3593-3600 NN denotes cytosol
T1949 3601-3603 IN denotes as
T1950 3604-3605 DT denotes a
T1951 3606-3618 RB denotes biologically
T1952 3619-3627 JJ denotes inactive
T1953 3628-3639 JJ denotes full-length
T1954 3640-3649 NN denotes pro-IL-1β
T1955 3649-3650 . denotes .
T1956 3651-3660 NNP denotes Pro-IL-1β
T1957 3661-3663 VBZ denotes is
T1958 3664-3676 RB denotes subsequently
T1959 3677-3686 VBN denotes converted
T1960 3687-3691 IN denotes into
T1961 3692-3695 PRP$ denotes its
T1962 3696-3702 JJ denotes active
T1963 3703-3707 NN denotes form
T1964 3708-3710 IN denotes by
T1965 3711-3720 JJ denotes cytosolic
T1966 3721-3728 NN denotes protein
T1967 3729-3738 NNS denotes complexes
T1968 3739-3745 VBD denotes termed
T1969 3747-3760 NNS denotes inflammasomes
T1970 3760-3761 . denotes .
T1971 3763-3776 NNS denotes Inflammasomes
T1972 3777-3785 VBP denotes assemble
T1973 3786-3788 IN denotes in
T1974 3789-3797 NN denotes response
T1975 3798-3800 TO denotes to
T1976 3801-3808 JJ denotes certain
T1977 3809-3817 JJ denotes cellular
T1978 3818-3824 NN denotes danger
T1979 3825-3832 NNS denotes signals
T1980 3833-3836 CC denotes and
T1981 3837-3844 VB denotes mediate
T1982 3845-3848 DT denotes the
T1983 3849-3864 NN denotes auto-activation
T1984 3865-3867 IN denotes of
T1985 3868-3877 JJ denotes caspase-1
T1986 3878-3879 NN denotes [
T1987 3879-3880 CD denotes 9
T1988 3880-3881 NNP denotes ]
T1989 3881-3882 , denotes ,
T1990 3883-3884 NNP denotes [
T1991 3884-3886 CD denotes 11
T1992 3886-3887 NNP denotes ]
T1993 3887-3888 , denotes ,
T1994 3889-3894 WDT denotes which
T1995 3895-3902 VBZ denotes cleaves
T1996 3903-3912 NN denotes pro-IL-1β
T1997 3913-3916 CC denotes and
T1998 3917-3926 NN denotes pro-IL-18
T1999 3927-3931 IN denotes into
T2000 3932-3937 PRP$ denotes their
T2001 3938-3950 RB denotes biologically
T2002 3951-3957 JJ denotes active
T2003 3958-3963 NNS denotes forms
T2004 3964-3967 IN denotes for
T2005 3968-3977 NN denotes secretion
T2006 3977-3978 . denotes .
T2007 3979-3983 CD denotes Four
T2008 3984-3992 JJ denotes distinct
T2009 3993-4006 NNS denotes inflammasomes
T2010 4007-4011 VBP denotes have
T2011 4012-4016 VBN denotes been
T2012 4017-4027 VBN denotes recognized
T2013 4027-4028 . denotes .
T2014 4029-4034 DT denotes These
T2015 4035-4038 VBP denotes are
T2016 4039-4042 DT denotes the
T2017 4043-4048 NNP denotes NLRP1
T2018 4049-4050 NNP denotes [
T2019 4050-4052 CD denotes 12
T2020 4052-4053 NNP denotes ]
T2021 4053-4054 , denotes ,
T2022 4055-4060 NNP denotes NLRP3
T2023 4061-4062 NNP denotes [
T2024 4062-4064 CD denotes 13
T2025 4064-4065 NNP denotes ]
T2026 4065-4066 , denotes ,
T2027 4067-4068 NNP denotes [
T2028 4068-4070 CD denotes 14
T2029 4070-4071 NNP denotes ]
T2030 4071-4072 , denotes ,
T2031 4073-4078 NNP denotes NLRC4
T2032 4079-4080 NNP denotes [
T2033 4080-4082 CD denotes 15
T2034 4082-4083 NNP denotes ]
T2035 4083-4084 , denotes ,
T2036 4085-4086 NNP denotes [
T2037 4086-4088 CD denotes 16
T2038 4088-4089 NNP denotes ]
T2039 4089-4090 , denotes ,
T2040 4091-4094 CC denotes and
T2041 4095-4099 NNP denotes AIM2
T2042 4100-4113 VBZ denotes inflammasomes
T2043 4114-4115 NNP denotes [
T2044 4115-4117 CD denotes 17
T2045 4117-4118 NNP denotes ]
T2046 4118-4119 , denotes ,
T2047 4120-4121 NNP denotes [
T2048 4121-4123 CD denotes 18
T2049 4123-4124 NNP denotes ]
T2050 4124-4125 , denotes ,
T2051 4126-4131 WDT denotes which
T2052 4132-4139 VBP denotes respond
T2053 4140-4142 TO denotes to
T2054 4143-4144 DT denotes a
T2055 4145-4152 NN denotes variety
T2056 4153-4155 IN denotes of
T2057 4156-4165 JJ denotes different
T2058 4166-4175 JJ denotes microbial
T2059 4176-4186 NNS denotes signatures
T2060 4187-4190 CC denotes and
T2061 4191-4197 NN denotes danger
T2062 4198-4205 NNS denotes signals
T2063 4206-4207 NNP denotes [
T2064 4207-4209 CD denotes 11
T2065 4209-4210 NNP denotes ]
T2066 4210-4211 . denotes .
T2067 4212-4214 NNP denotes P.
T2068 4215-4225 NN denotes aeruginosa
T2069 4225-4226 , denotes ,
T2070 4227-4230 CD denotes one
T2071 4231-4233 IN denotes of
T2072 4234-4237 DT denotes the
T2073 4238-4242 RBS denotes most
T2074 4243-4249 JJ denotes common
T2075 4250-4253 CC denotes and
T2076 4254-4264 RB denotes clinically
T2077 4265-4273 JJ denotes relevant
T2078 4274-4283 NNS denotes pathogens
T2079 4284-4289 IN denotes among
T2080 4290-4292 NNP denotes CF
T2081 4293-4301 NNS denotes patients
T2082 4301-4302 , denotes ,
T2083 4303-4312 VBZ denotes activates
T2084 4313-4316 DT denotes the
T2085 4317-4322 NNP denotes NLRC4
T2086 4323-4335 JJ denotes inflammasome
T2087 4336-4337 NNP denotes [
T2088 4337-4339 CD denotes 19
T2089 4339-4340 NNP denotes ]
T2090 4340-4341 , denotes ,
T2091 4342-4343 NNP denotes [
T2092 4343-4345 CD denotes 20
T2093 4345-4346 NNP denotes ]
T2094 4346-4347 . denotes .
T2095 4348-4357 NN denotes Infection
T2096 4358-4362 IN denotes with
T2097 4363-4365 NNP denotes P.
T2098 4366-4376 NN denotes aeruginosa
T2099 4377-4385 VBZ denotes triggers
T2100 4386-4388 DT denotes an
T2101 4389-4397 NN denotes increase
T2102 4398-4400 IN denotes in
T2103 4401-4407 NNS denotes levels
T2104 4408-4410 IN denotes of
T2105 4411-4416 NN denotes IL-1β
T2106 4416-4417 , denotes ,
T2107 4418-4422 NNP denotes IL-6
T2108 4422-4423 , denotes ,
T2109 4424-4427 CC denotes and
T2110 4428-4432 NNP denotes IL-8
T2111 4433-4435 IN denotes in
T2112 4436-4451 JJ denotes bronchoalveolar
T2113 4452-4458 NN denotes lavage
T2114 4459-4464 NN denotes fluid
T2115 4465-4466 -LRB- denotes (
T2116 4466-4470 NNP denotes BALF
T2117 4470-4471 -RRB- denotes )
T2118 4472-4476 IN denotes from
T2119 4477-4485 NNS denotes patients
T2120 4486-4490 IN denotes with
T2121 4491-4493 NNP denotes CF
T2122 4494-4495 NNP denotes [
T2123 4495-4497 CD denotes 21
T2124 4497-4498 NNP denotes ]
T2125 4498-4499 . denotes .
T2126 4500-4512 JJ denotes Inflammasome
T2127 4513-4522 NNS denotes responses
T2128 4523-4529 VBP denotes depend
T2129 4530-4532 IN denotes on
T2130 4533-4538 JJ denotes NF-κB
T2131 4539-4548 VBG denotes signaling
T2132 4548-4549 , denotes ,
T2133 4550-4555 WRB denotes where
T2134 4556-4561 NNP denotes NF-κB
T2135 4562-4564 VBZ denotes is
T2136 4565-4574 JJ denotes important
T2137 4575-4577 IN denotes in
T2138 4578-4582 DT denotes both
T2139 4583-4586 DT denotes the
T2140 4587-4599 NN denotes upregulation
T2141 4600-4602 IN denotes of
T2142 4603-4611 JJ denotes specific
T2143 4612-4624 JJ denotes inflammasome
T2144 4625-4635 NNS denotes components
T2145 4636-4637 NNP denotes [
T2146 4637-4639 CD denotes 22
T2147 4639-4640 NNP denotes ]
T2148 4640-4641 , denotes ,
T2149 4642-4643 NNP denotes [
T2150 4643-4645 CD denotes 23
T2151 4645-4646 NNP denotes ]
T2152 4646-4647 , denotes ,
T2153 4648-4650 RB denotes as
T2154 4651-4655 RB denotes well
T2155 4656-4658 IN denotes as
T2156 4659-4664 JJ denotes IL-1β
T2157 4665-4675 NN denotes expression
T2158 4676-4677 NNP denotes [
T2159 4677-4679 CD denotes 24
T2160 4679-4680 NNP denotes ]
T2161 4680-4681 , denotes ,
T2162 4682-4683 NNP denotes [
T2163 4683-4685 CD denotes 25
T2164 4685-4686 NNP denotes ]
T2165 4686-4687 . denotes .
T2166 4688-4696 JJ denotes Previous
T2167 4697-4704 NNS denotes studies
T2168 4705-4712 VBP denotes support
T2169 4713-4714 DT denotes a
T2170 4715-4719 NN denotes role
T2171 4720-4723 IN denotes for
T2172 4724-4729 NN denotes IL-1β
T2173 4730-4732 IN denotes in
T2174 4733-4736 DT denotes the
T2175 4737-4749 NN denotes pathogenesis
T2176 4750-4752 IN denotes of
T2177 4753-4755 NNP denotes CF
T2178 4756-4768 JJ denotes inflammatory
T2179 4769-4773 NN denotes lung
T2180 4774-4781 NN denotes disease
T2181 4781-4782 . denotes .
T2182 4783-4789 NNS denotes Levels
T2183 4790-4792 IN denotes of
T2184 4793-4798 NN denotes IL-1β
T2185 4799-4802 VBP denotes are
T2186 4803-4812 VBN denotes increased
T2187 4813-4815 IN denotes in
T2188 4816-4820 NNP denotes BALF
T2189 4821-4825 IN denotes from
T2190 4826-4828 NNP denotes CF
T2191 4829-4837 NNS denotes patients
T2192 4838-4842 IN denotes with
T2193 4843-4852 NN denotes infection
T2194 4853-4854 NNP denotes [
T2195 4854-4856 CD denotes 21
T2196 4856-4857 NNP denotes ]
T2197 4857-4858 , denotes ,
T2198 4859-4860 NNP denotes [
T2199 4860-4862 CD denotes 26
T2200 4862-4863 NNP denotes ]
T2201 4863-4864 , denotes ,
T2202 4865-4866 NNP denotes [
T2203 4866-4868 CD denotes 27
T2204 4868-4869 NNP denotes ]
T2205 4869-4870 , denotes ,
T2206 4871-4872 NNP denotes [
T2207 4872-4874 CD denotes 28
T2208 4874-4875 NNP denotes ]
T2209 4876-4879 CC denotes and
T2210 4880-4884 DT denotes this
T2211 4885-4893 NN denotes increase
T2212 4894-4897 VBZ denotes has
T2213 4898-4902 VBN denotes been
T2214 4903-4913 RB denotes temporally
T2215 4914-4924 VBN denotes associated
T2216 4925-4929 IN denotes with
T2217 4930-4931 DT denotes a
T2218 4932-4940 JJ denotes clinical
T2219 4941-4949 NN denotes response
T2220 4950-4952 TO denotes to
T2221 4953-4962 NN denotes treatment
T2222 4963-4964 NNP denotes [
T2223 4964-4966 CD denotes 21
T2224 4966-4967 NNP denotes ]
T2225 4967-4968 . denotes .
T2226 4969-4982 NNS denotes Polymorphisms
T2227 4983-4985 IN denotes in
T2228 4986-4989 DT denotes the
T2229 4990-4994 NNP denotes IL1B
T2230 4995-4999 NN denotes gene
T2231 5000-5004 VBP denotes have
T2232 5005-5009 RB denotes also
T2233 5010-5014 VBN denotes been
T2234 5015-5025 VBN denotes associated
T2235 5026-5030 IN denotes with
T2236 5031-5038 VBG denotes varying
T2237 5039-5046 NNS denotes degrees
T2238 5047-5049 IN denotes of
T2239 5050-5057 NN denotes disease
T2240 5058-5066 NN denotes severity
T2241 5067-5069 IN denotes in
T2242 5070-5072 NNP denotes CF
T2243 5073-5081 NNS denotes patients
T2244 5082-5083 NNP denotes [
T2245 5083-5085 CD denotes 29
T2246 5085-5086 NNP denotes ]
T2247 5086-5087 . denotes .
T2248 5088-5094 NNP denotes Murine
T2249 5095-5101 NNS denotes models
T2250 5102-5104 IN denotes of
T2251 5105-5109 NNP denotes CFTR
T2252 5110-5121 NN denotes dysfunction
T2253 5122-5126 VBP denotes have
T2254 5127-5136 VBN denotes exhibited
T2255 5137-5148 JJ denotes significant
T2256 5149-5158 NNS denotes increases
T2257 5159-5161 IN denotes in
T2258 5162-5167 JJ denotes IL-1β
T2259 5168-5178 NN denotes expression
T2260 5179-5181 CC denotes or
T2261 5182-5191 NN denotes secretion
T2262 5192-5194 IN denotes in
T2263 5195-5206 NNS denotes macrophages
T2264 5207-5208 NNP denotes [
T2265 5208-5210 CD denotes 30
T2266 5210-5211 NNP denotes ]
T2267 5211-5212 , denotes ,
T2268 5213-5214 NNP denotes [
T2269 5214-5216 CD denotes 31
T2270 5216-5217 NNP denotes ]
T2271 5217-5218 , denotes ,
T2272 5219-5222 CC denotes and
T2273 5223-5230 VB denotes support
T2274 5231-5234 DT denotes the
T2275 5235-5245 NN denotes hypothesis
T2276 5246-5250 IN denotes that
T2277 5251-5254 DT denotes the
T2278 5255-5259 NN denotes loss
T2279 5260-5262 IN denotes of
T2280 5263-5267 NNP denotes CFTR
T2281 5268-5277 VBZ denotes increases
T2282 5278-5283 JJ denotes NF-κB
T2283 5284-5294 NN denotes activation
T2284 5295-5300 IN denotes under
T2285 5301-5306 NN denotes basal
T2286 5307-5310 CC denotes and
T2287 5311-5322 JJ denotes stimulatory
T2288 5323-5333 NNS denotes conditions
T2289 5334-5335 VBP denotes [
T2290 5335-5336 CD denotes 4
T2291 5336-5337 NNP denotes ]
T2292 5337-5338 , denotes ,
T2293 5339-5340 NNP denotes [
T2294 5340-5341 CD denotes 5
T2295 5341-5342 NNP denotes ]
T2296 5342-5343 , denotes ,
T2297 5344-5345 NNP denotes [
T2298 5345-5347 CD denotes 32
T2299 5347-5348 NNP denotes ]
T2300 5348-5349 , denotes ,
T2301 5350-5351 NNP denotes [
T2302 5351-5353 CD denotes 33
T2303 5353-5354 NNP denotes ]
T2304 5354-5355 . denotes .
T2305 5356-5363 RB denotes Finally
T2306 5363-5364 , denotes ,
T2307 5365-5376 NN denotes replacement
T2308 5377-5379 IN denotes of
T2309 5380-5388 NN denotes chloride
T2310 5389-5393 NNS denotes ions
T2311 5394-5398 IN denotes with
T2312 5399-5408 NN denotes glutamate
T2313 5409-5411 CC denotes or
T2314 5412-5421 NN denotes gluconate
T2315 5422-5424 IN denotes in
T2316 5425-5429 NN denotes cell
T2317 5430-5437 NN denotes culture
T2318 5438-5443 NNS denotes media
T2319 5444-5453 VBZ denotes increases
T2320 5454-5463 NN denotes secretion
T2321 5464-5466 IN denotes of
T2322 5467-5472 NN denotes IL-1β
T2323 5473-5475 IN denotes in
T2324 5476-5484 NN denotes response
T2325 5485-5487 TO denotes to
T2326 5488-5493 CD denotes NLRP3
T2327 5494-5505 NN denotes stimulation
T2328 5506-5508 IN denotes by
T2329 5509-5518 JJ denotes adenosine
T2330 5519-5531 NN denotes triphosphate
T2331 5532-5533 -LRB- denotes (
T2332 5533-5536 NNP denotes ATP
T2333 5536-5537 -RRB- denotes )
T2334 5538-5539 NNP denotes [
T2335 5539-5541 CD denotes 34
T2336 5541-5542 NNP denotes ]
T2337 5542-5543 , denotes ,
T2338 5544-5552 VBG denotes implying
T2339 5553-5555 DT denotes an
T2340 5556-5566 JJ denotes inhibitory
T2341 5567-5571 NN denotes role
T2342 5572-5575 IN denotes for
T2343 5576-5589 JJ denotes extracellular
T2344 5590-5598 NN denotes chloride
T2345 5599-5601 IN denotes in
T2346 5602-5607 CD denotes NLRP3
T2347 5608-5618 NN denotes activation
T2348 5618-5619 . denotes .
T2349 5620-5625 VBN denotes Taken
T2350 5626-5634 RB denotes together
T2351 5634-5635 , denotes ,
T2352 5636-5641 DT denotes these
T2353 5642-5646 NNS denotes data
T2354 5647-5656 VBP denotes implicate
T2355 5657-5660 DT denotes the
T2356 5661-5672 NN denotes involvement
T2357 5673-5675 IN denotes of
T2358 5676-5681 NN denotes IL-1β
T2359 5682-5685 CC denotes and
T2360 5686-5698 RB denotes consequently
T2361 5698-5699 , denotes ,
T2362 5700-5703 DT denotes the
T2363 5704-5717 NNS denotes inflammasomes
T2364 5717-5718 , denotes ,
T2365 5719-5721 IN denotes in
T2366 5722-5724 NNP denotes CF
T2367 5725-5737 JJ denotes inflammatory
T2368 5738-5745 NN denotes disease
T2369 5745-5746 . denotes .
T2806 5757-5763 NNP denotes Airway
T2807 5764-5774 JJ denotes epithelial
T2808 5775-5780 NNS denotes cells
T2809 5781-5783 VBP denotes do
T2810 5784-5787 RB denotes not
T2811 5788-5795 VB denotes produce
T2812 5796-5807 JJ denotes significant
T2813 5808-5815 NNS denotes amounts
T2814 5816-5818 IN denotes of
T2815 5819-5824 NN denotes IL-1β
T2816 5825-5827 IN denotes in
T2817 5828-5836 NN denotes response
T2818 5837-5839 TO denotes to
T2819 5840-5852 JJ denotes inflammasome
T2820 5853-5864 NN denotes stimulation
T2821 5865-5868 DT denotes The
T2822 5869-5882 NNS denotes inflammasomes
T2823 5883-5886 CC denotes and
T2824 5887-5892 PRP$ denotes their
T2825 5893-5903 JJ denotes respective
T2826 5904-5914 NNS denotes activators
T2827 5915-5923 VBD denotes examined
T2828 5924-5926 IN denotes in
T2829 5927-5931 DT denotes this
T2830 5932-5937 NN denotes study
T2831 5938-5941 VBP denotes are
T2832 5942-5948 VBN denotes listed
T2833 5949-5951 IN denotes in
T2834 5952-5957 NNP denotes Table
T2835 5958-5959 CD denotes 1
T2836 5959-5960 . denotes .
T2837 5961-5966 NNS denotes Cells
T2838 5967-5971 VBD denotes were
T2839 5972-5982 VBN denotes stimulated
T2840 5983-5985 IN denotes in
T2841 5986-5996 NN denotes accordance
T2842 5997-6001 IN denotes with
T2843 6002-6005 DT denotes the
T2844 6006-6014 NN denotes schedule
T2845 6015-6017 IN denotes in
T2846 6018-6024 NN denotes Figure
T2847 6025-6026 CD denotes 1
T2848 6026-6027 . denotes .
T2849 6028-6030 IN denotes In
T2850 6031-6033 NNP denotes CF
T2851 6033-6034 , denotes ,
T2852 6035-6041 NN denotes airway
T2853 6042-6052 JJ denotes epithelial
T2854 6053-6058 NNS denotes cells
T2855 6059-6063 VBP denotes have
T2856 6064-6068 VBN denotes been
T2857 6069-6074 VBN denotes shown
T2858 6075-6077 TO denotes to
T2859 6078-6085 VB denotes possess
T2860 6086-6087 DT denotes a
T2861 6088-6106 JJ denotes hyper-inflammatory
T2862 6107-6116 NN denotes phenotype
T2863 6117-6120 CC denotes and
T2864 6121-6128 VBP denotes produce
T2865 6129-6131 DT denotes an
T2866 6132-6143 JJ denotes exaggerated
T2867 6144-6160 JJ denotes pro-inflammatory
T2868 6161-6169 NN denotes cytokine
T2869 6170-6178 NN denotes response
T2870 6179-6180 NNP denotes [
T2871 6180-6181 CD denotes 4
T2872 6181-6182 NNP denotes ]
T2873 6182-6183 , denotes ,
T2874 6184-6185 NNP denotes [
T2875 6185-6186 CD denotes 5
T2876 6186-6187 NNP denotes ]
T2877 6187-6188 . denotes .
T2878 6189-6191 TO denotes To
T2879 6192-6201 VB denotes determine
T2880 6202-6204 IN denotes if
T2881 6205-6211 NN denotes airway
T2882 6212-6222 JJ denotes epithelial
T2883 6223-6228 NNS denotes cells
T2884 6229-6239 VBP denotes contribute
T2885 6240-6242 TO denotes to
T2886 6243-6246 DT denotes the
T2887 6247-6256 VBN denotes increased
T2888 6257-6262 NN denotes IL-1β
T2889 6263-6273 NN denotes production
T2890 6274-6276 IN denotes in
T2891 6277-6285 NNS denotes patients
T2892 6286-6290 IN denotes with
T2893 6291-6293 NNP denotes CF
T2894 6293-6294 , denotes ,
T2895 6295-6297 NNP denotes CF
T2896 6298-6301 CC denotes and
T2897 6302-6309 VB denotes control
T2898 6310-6319 JJ denotes bronchial
T2899 6320-6330 JJ denotes epithelial
T2900 6331-6335 NN denotes cell
T2901 6336-6341 NNS denotes lines
T2902 6342-6346 VBD denotes were
T2903 6347-6357 VBN denotes stimulated
T2904 6358-6362 IN denotes with
T2905 6363-6366 DT denotes the
T2906 6367-6379 JJ denotes inflammasome
T2907 6380-6388 NNS denotes inducers
T2908 6389-6391 NNP denotes P.
T2909 6392-6402 NN denotes aeruginosa
T2910 6403-6409 NN denotes strain
T2911 6410-6414 NNP denotes PAO1
T2912 6415-6416 -LRB- denotes (
T2913 6416-6420 NNP denotes PAO1
T2914 6420-6421 -RRB- denotes )
T2915 6422-6425 CC denotes and
T2916 6426-6429 NNP denotes LPS
T2917 6430-6438 VBD denotes followed
T2918 6439-6441 IN denotes by
T2919 6442-6445 NNP denotes ATP
T2920 6445-6446 . denotes .
T2921 6447-6452 JJ denotes IL-1β
T2922 6453-6459 NNS denotes levels
T2923 6460-6462 IN denotes in
T2924 6463-6467 NN denotes cell
T2925 6468-6475 NN denotes culture
T2926 6476-6488 NNS denotes supernatants
T2927 6489-6493 VBD denotes were
T2928 6494-6497 RB denotes not
T2929 6498-6505 RB denotes greatly
T2930 6506-6515 VBN denotes increased
T2931 6516-6518 IN denotes in
T2932 6519-6525 CC denotes either
T2933 6526-6529 DT denotes the
T2934 6530-6532 NNP denotes CF
T2935 6533-6535 CC denotes or
T2936 6536-6543 VB denotes control
T2937 6544-6548 NN denotes cell
T2938 6549-6554 NNS denotes lines
T2939 6555-6556 -LRB- denotes (
T2940 6556-6560 NNP denotes Fig.
T2941 6561-6563 CD denotes 2a
T2942 6564-6565 LS denotes d
T2943 6565-6566 -RRB- denotes )
T2944 6566-6567 , denotes ,
T2945 6568-6576 IN denotes although
T2946 6577-6578 DT denotes a
T2947 6579-6584 JJ denotes small
T2948 6585-6593 NN denotes increase
T2949 6594-6596 IN denotes in
T2950 6597-6602 JJ denotes IL-1β
T2951 6603-6613 NN denotes production
T2952 6614-6617 VBD denotes was
T2953 6618-6626 VBN denotes detected
T2954 6627-6629 IN denotes in
T2955 6630-6636 NN denotes NuLi-1
T2956 6637-6640 CC denotes and
T2957 6641-6647 NN denotes CuFi-1
T2958 6648-6653 NNS denotes cells
T2959 6653-6654 , denotes ,
T2960 6655-6658 CC denotes but
T2961 6659-6662 RB denotes not
T2962 6663-6665 IN denotes in
T2963 6666-6668 CD denotes S9
T2964 6669-6672 CC denotes and
T2965 6673-6678 CD denotes IB3-1
T2966 6679-6684 NNS denotes cells
T2967 6684-6685 , denotes ,
T2968 6686-6688 IN denotes by
T2969 6689-6691 CD denotes 24
T2970 6692-6697 NNS denotes hours
T2971 6697-6698 . denotes .
T2972 6699-6701 IN denotes In
T2973 6702-6710 NN denotes contrast
T2974 6710-6711 , denotes ,
T2975 6712-6717 DT denotes these
T2976 6718-6724 NN denotes airway
T2977 6725-6730 NNS denotes cells
T2978 6731-6735 VBD denotes were
T2979 6736-6742 RB denotes highly
T2980 6743-6753 JJ denotes responsive
T2981 6754-6756 TO denotes to
T2982 6757-6762 JJ denotes other
T2983 6763-6775 JJ denotes inflammatory
T2984 6776-6783 NNS denotes stimuli
T2985 6783-6784 , denotes ,
T2986 6785-6789 JJ denotes such
T2987 6790-6792 IN denotes as
T2988 6793-6804 JJ denotes recombinant
T2989 6805-6810 NNP denotes IL-1β
T2990 6810-6811 , denotes ,
T2991 6812-6821 VBG denotes producing
T2992 6822-6827 JJ denotes large
T2993 6828-6838 NNS denotes quantities
T2994 6839-6841 IN denotes of
T2995 6842-6846 NN denotes IL-8
T2996 6847-6848 -LRB- denotes (
T2997 6848-6852 NNP denotes Fig.
T2998 6853-6855 CD denotes 2a
T2999 6856-6857 LS denotes d
T3000 6858-6865 NNS denotes inserts
T3001 6865-6866 -RRB- denotes )
T3002 6866-6867 . denotes .
T3214 7936-7942 NNP denotes Airway
T3215 7943-7953 JJ denotes epithelial
T3216 7954-7959 NNS denotes cells
T3217 7960-7962 VBP denotes do
T3218 7963-7966 RB denotes not
T3219 7967-7980 RB denotes significantly
T3220 7981-7991 VB denotes upregulate
T3221 7992-8001 JJ denotes caspase-1
T3222 8002-8010 NN denotes activity
T3223 8011-8013 IN denotes in
T3224 8014-8022 NN denotes response
T3225 8023-8025 TO denotes to
T3226 8026-8038 JJ denotes inflammasome
T3227 8039-8050 NN denotes stimulation
T3228 8051-8053 TO denotes To
T3229 8054-8061 VB denotes examine
T3230 8062-8064 IN denotes if
T3231 8065-8077 JJ denotes inflammasome
T3232 8078-8088 NN denotes activation
T3233 8089-8095 VBZ denotes occurs
T3234 8096-8098 IN denotes in
T3235 8099-8104 DT denotes these
T3236 8105-8111 NN denotes airway
T3237 8112-8117 NNS denotes cells
T3238 8117-8118 , denotes ,
T3239 8119-8128 JJ denotes caspase-1
T3240 8129-8137 NN denotes activity
T3241 8138-8141 VBD denotes was
T3242 8142-8152 VBN denotes quantified
T3243 8153-8155 IN denotes by
T3244 8156-8160 NN denotes flow
T3245 8161-8170 NN denotes cytometry
T3246 8170-8171 . denotes .
T3247 8172-8177 EX denotes There
T3248 8178-8181 VBD denotes was
T3249 8182-8184 DT denotes no
T3250 8185-8196 JJ denotes significant
T3251 8197-8205 NN denotes increase
T3252 8206-8210 IN denotes upon
T3253 8211-8222 NN denotes stimulation
T3254 8223-8227 IN denotes with
T3255 8228-8232 JJ denotes live
T3256 8233-8237 CD denotes PAO1
T3257 8238-8240 CC denotes or
T3258 8241-8248 NNP denotes LPS+ATP
T3259 8249-8251 IN denotes at
T3260 8252-8255 DT denotes the
T3261 8256-8261 NNS denotes times
T3262 8262-8270 VBD denotes examined
T3263 8271-8272 -LRB- denotes (
T3264 8272-8276 NNP denotes Fig.
T3265 8277-8279 CD denotes 3a
T3266 8280-8281 NN denotes b
T3267 8281-8282 -RRB- denotes )
T3268 8282-8283 . denotes .
T3269 8284-8291 IN denotes Because
T3270 8292-8300 JJ denotes previous
T3271 8301-8308 NNS denotes studies
T3272 8309-8313 VBP denotes have
T3273 8314-8323 VBN denotes indicated
T3274 8324-8325 DT denotes a
T3275 8326-8330 NN denotes role
T3276 8331-8334 IN denotes for
T3277 8335-8344 JJ denotes caspase-1
T3278 8345-8347 IN denotes in
T3279 8348-8351 DT denotes the
T3280 8352-8362 NN denotes activation
T3281 8363-8365 IN denotes of
T3282 8366-8371 NN denotes NF-κB
T3283 8372-8379 IN denotes through
T3284 8380-8389 JJ denotes Toll-like
T3285 8390-8398 NN denotes receptor
T3286 8399-8400 -LRB- denotes (
T3287 8400-8403 NNP denotes TLR
T3288 8403-8404 -RRB- denotes )
T3289 8405-8414 VBG denotes signaling
T3290 8415-8416 NNP denotes [
T3291 8416-8418 CD denotes 35
T3292 8418-8419 NNP denotes ]
T3293 8419-8420 , denotes ,
T3294 8421-8423 PRP denotes we
T3295 8424-8432 VBD denotes examined
T3296 8433-8440 IN denotes whether
T3297 8441-8449 NN denotes chemical
T3298 8450-8460 NN denotes inhibition
T3299 8461-8463 IN denotes of
T3300 8464-8473 JJ denotes caspase-1
T3301 8474-8481 JJ denotes altered
T3302 8482-8497 JJ denotes NF-κB-dependent
T3303 8498-8502 NN denotes IL-6
T3304 8503-8513 NN denotes production
T3305 8514-8516 IN denotes in
T3306 8517-8525 NN denotes response
T3307 8526-8528 TO denotes to
T3308 8529-8531 NNP denotes P.
T3309 8532-8542 NN denotes aeruginosa
T3310 8542-8543 . denotes .
T3311 8544-8551 RB denotes However
T3312 8551-8552 , denotes ,
T3313 8553-8562 NN denotes treatment
T3314 8563-8567 IN denotes with
T3315 8568-8571 DT denotes the
T3316 8572-8581 JJ denotes caspase-1
T3317 8582-8591 NN denotes inhibitor
T3318 8592-8602 NNP denotes z-YVAD-fmk
T3319 8603-8604 -LRB- denotes (
T3320 8604-8608 NNP denotes YVAD
T3321 8608-8609 -RRB- denotes )
T3322 8610-8613 VBD denotes did
T3323 8614-8617 RB denotes not
T3324 8618-8626 VB denotes decrease
T3325 8627-8631 NNP denotes IL-6
T3326 8632-8641 NN denotes secretion
T3327 8642-8644 IN denotes by
T3328 8645-8651 NN denotes airway
T3329 8652-8662 JJ denotes epithelial
T3330 8663-8668 NNS denotes cells
T3331 8669-8670 -LRB- denotes (
T3332 8670-8674 NNP denotes Fig.
T3333 8675-8677 CD denotes 3c
T3334 8677-8678 -RRB- denotes )
T3335 8678-8679 . denotes .
T3485 9425-9429 CD denotes CD14
T3486 9430-9438 JJ denotes positive
T3487 9439-9448 NNS denotes monocytes
T3488 9449-9453 IN denotes from
T3489 9454-9456 NNP denotes CF
T3490 9457-9465 NNS denotes patients
T3491 9466-9469 CC denotes and
T3492 9470-9478 NNS denotes controls
T3493 9479-9483 VBP denotes show
T3494 9484-9491 JJ denotes similar
T3495 9492-9501 NNS denotes increases
T3496 9502-9504 IN denotes in
T3497 9505-9514 JJ denotes caspase-1
T3498 9515-9523 NN denotes activity
T3499 9524-9528 IN denotes upon
T3500 9529-9541 JJ denotes inflammasome
T3501 9542-9553 NN denotes stimulation
T3502 9554-9563 NNS denotes Monocytes
T3503 9564-9568 VBD denotes were
T3504 9569-9579 VBN denotes identified
T3505 9580-9582 IN denotes in
T3506 9583-9587 NNP denotes PBMC
T3507 9588-9599 NNS denotes populations
T3508 9600-9605 VBG denotes using
T3509 9606-9610 NNP denotes CD14
T3510 9611-9613 IN denotes as
T3511 9614-9615 DT denotes a
T3512 9616-9627 VBG denotes phenotyping
T3513 9628-9634 NN denotes marker
T3514 9634-9635 . denotes .
T3515 9636-9640 CD denotes CD14
T3516 9641-9649 JJ denotes positive
T3517 9650-9659 NNS denotes monocytes
T3518 9660-9664 IN denotes from
T3519 9665-9667 NNP denotes CF
T3520 9668-9676 NNS denotes patients
T3521 9677-9680 CC denotes and
T3522 9681-9688 JJ denotes healthy
T3523 9689-9697 NNS denotes controls
T3524 9698-9704 VBD denotes showed
T3525 9705-9706 DT denotes a
T3526 9707-9718 JJ denotes significant
T3527 9719-9727 NN denotes increase
T3528 9728-9730 IN denotes in
T3529 9731-9740 JJ denotes caspase-1
T3530 9741-9751 NN denotes activation
T3531 9752-9756 IN denotes upon
T3532 9757-9768 NN denotes stimulation
T3533 9769-9773 IN denotes with
T3534 9774-9781 NNP denotes LPS+ATP
T3535 9781-9782 , denotes ,
T3536 9783-9787 NNP denotes PAO1
T3537 9787-9788 , denotes ,
T3538 9789-9792 CC denotes and
T3539 9793-9798 NNP denotes LPS+P
T3540 9798-9801 NN denotes oly
T3541 9801-9802 -LRB- denotes (
T3542 9802-9804 NN denotes dA
T3543 9804-9805 : denotes :
T3544 9805-9807 NN denotes dT
T3545 9807-9808 -RRB- denotes )
T3546 9809-9810 -LRB- denotes (
T3547 9810-9814 NNP denotes Fig.
T3548 9815-9817 CD denotes 4a
T3549 9817-9818 -RRB- denotes )
T3550 9819-9822 CC denotes but
T3551 9823-9827 DT denotes this
T3552 9828-9838 NN denotes activation
T3553 9839-9842 VBD denotes was
T3554 9843-9846 RB denotes not
T3555 9847-9856 JJ denotes different
T3556 9857-9864 IN denotes between
T3557 9865-9867 NNP denotes CF
T3558 9868-9871 CC denotes and
T3559 9872-9879 VB denotes control
T3560 9880-9888 NNS denotes subjects
T3561 9889-9890 -LRB- denotes (
T3562 9890-9894 NNP denotes Fig.
T3563 9895-9897 CD denotes 4b
T3564 9897-9898 -RRB- denotes )
T3565 9898-9899 . denotes .
T3916 10426-10431 NNS denotes PBMCs
T3917 10432-10436 IN denotes from
T3918 10437-10439 NNP denotes CF
T3919 10440-10448 NNS denotes patients
T3920 10449-10451 VBP denotes do
T3921 10452-10455 RB denotes not
T3922 10456-10463 VB denotes produce
T3923 10464-10473 JJ denotes increased
T3924 10474-10481 NNS denotes amounts
T3925 10482-10484 IN denotes of
T3926 10485-10490 NN denotes IL-1β
T3927 10491-10495 IN denotes upon
T3928 10496-10508 JJ denotes inflammasome
T3929 10509-10520 NN denotes stimulation
T3930 10521-10529 JJ denotes Previous
T3931 10530-10537 NNS denotes studies
T3932 10538-10542 VBP denotes have
T3933 10543-10548 VBN denotes shown
T3934 10549-10553 IN denotes that
T3935 10554-10557 DT denotes the
T3936 10558-10562 NN denotes loss
T3937 10563-10565 IN denotes of
T3938 10566-10570 NNP denotes CFTR
T3939 10571-10578 NNS denotes results
T3940 10579-10581 IN denotes in
T3941 10582-10591 VBN denotes increased
T3942 10592-10597 NN denotes NF-κB
T3943 10598-10606 NN denotes activity
T3944 10607-10610 CC denotes and
T3945 10611-10627 JJ denotes pro-inflammatory
T3946 10628-10636 NN denotes cytokine
T3947 10637-10646 NN denotes secretion
T3948 10647-10648 NNP denotes [
T3949 10648-10649 CD denotes 4
T3950 10649-10650 NNP denotes ]
T3951 10650-10651 , denotes ,
T3952 10652-10653 NNP denotes [
T3953 10653-10654 CD denotes 5
T3954 10654-10655 NNP denotes ]
T3955 10655-10656 , denotes ,
T3956 10657-10658 NNP denotes [
T3957 10658-10660 CD denotes 32
T3958 10660-10661 NNP denotes ]
T3959 10661-10662 , denotes ,
T3960 10663-10664 NNP denotes [
T3961 10664-10666 CD denotes 36
T3962 10666-10667 NNP denotes ]
T3963 10667-10668 , denotes ,
T3964 10669-10670 NNP denotes [
T3965 10670-10672 CD denotes 37
T3966 10672-10673 NNP denotes ]
T3967 10673-10674 . denotes .
T3968 10675-10677 TO denotes To
T3969 10678-10685 RBR denotes further
T3970 10686-10693 VB denotes examine
T3971 10694-10698 DT denotes this
T3972 10699-10711 NN denotes relationship
T3973 10711-10712 , denotes ,
T3974 10713-10718 NNP denotes PBMCs
T3975 10719-10723 IN denotes from
T3976 10724-10726 NNP denotes CF
T3977 10727-10735 NNS denotes patients
T3978 10736-10739 CC denotes and
T3979 10740-10747 JJ denotes healthy
T3980 10748-10753 NN denotes adult
T3981 10754-10762 NNS denotes controls
T3982 10763-10767 VBD denotes were
T3983 10768-10778 VBN denotes stimulated
T3984 10779-10783 IN denotes with
T3985 10784-10788 NNP denotes PAO1
T3986 10788-10789 , denotes ,
T3987 10790-10797 NNP denotes LPS+ATP
T3988 10797-10798 , denotes ,
T3989 10799-10802 CC denotes and
T3990 10803-10808 NNP denotes LPS+P
T3991 10808-10811 NN denotes oly
T3992 10811-10812 -LRB- denotes (
T3993 10812-10814 NN denotes dA
T3994 10814-10815 : denotes :
T3995 10815-10817 NN denotes dT
T3996 10817-10818 -RRB- denotes )
T3997 10818-10819 , denotes ,
T3998 10820-10822 TO denotes to
T3999 10823-10831 VB denotes activate
T4000 10832-10835 DT denotes the
T4001 10836-10841 NNP denotes NLRC4
T4002 10841-10842 , denotes ,
T4003 10843-10848 NNP denotes NLRP3
T4004 10848-10849 , denotes ,
T4005 10850-10853 CC denotes and
T4006 10854-10858 NNP denotes AIM2
T4007 10859-10872 NNS denotes inflammasomes
T4008 10872-10873 , denotes ,
T4009 10874-10886 RB denotes respectively
T4010 10886-10887 . denotes .
T4011 10888-10890 IN denotes By
T4012 10891-10893 CD denotes 24
T4013 10894-10899 NNS denotes hours
T4014 10900-10902 IN denotes of
T4015 10903-10914 NN denotes stimulation
T4016 10914-10915 , denotes ,
T4017 10916-10918 NNP denotes CF
T4018 10919-10924 NNP denotes PBMCs
T4019 10925-10928 VBD denotes did
T4020 10929-10932 RB denotes not
T4021 10933-10940 VB denotes produce
T4022 10941-10950 JJ denotes increased
T4023 10951-10958 NNS denotes amounts
T4024 10959-10961 IN denotes of
T4025 10962-10967 NN denotes IL-1β
T4026 10968-10969 -LRB- denotes (
T4027 10969-10973 NNP denotes Fig.
T4028 10974-10976 CD denotes 5a
T4029 10976-10977 -RRB- denotes )
T4030 10978-10980 CC denotes or
T4031 10981-10985 NN denotes IL-8
T4032 10986-10987 -LRB- denotes (
T4033 10987-10991 NNP denotes Fig.
T4034 10992-10994 CD denotes 5b
T4035 10994-10995 -RRB- denotes )
T4036 10996-11000 WRB denotes when
T4037 11001-11009 VBN denotes compared
T4038 11010-11012 TO denotes to
T4039 11013-11020 JJ denotes healthy
T4040 11021-11029 NNS denotes controls
T4041 11029-11030 . denotes .
T4042 11031-11038 RB denotes However
T4043 11038-11039 , denotes ,
T4044 11040-11042 PRP denotes we
T4045 11043-11046 VBD denotes did
T4046 11047-11053 VB denotes notice
T4047 11054-11055 DT denotes a
T4048 11056-11065 NN denotes transient
T4049 11066-11074 NN denotes decrease
T4050 11075-11076 -LRB- denotes (
T4051 11076-11077 NNP denotes P
T4052 11077-11078 CD denotes <
T4053 11078-11083 CD denotes 0.001
T4054 11083-11084 -RRB- denotes )
T4055 11085-11087 IN denotes in
T4056 11088-11091 DT denotes the
T4057 11092-11098 NN denotes amount
T4058 11099-11101 IN denotes of
T4059 11102-11107 JJ denotes IL-1β
T4060 11108-11116 VBN denotes produced
T4061 11117-11119 IN denotes by
T4062 11120-11122 NNP denotes CF
T4063 11123-11128 NNS denotes cells
T4064 11129-11131 IN denotes in
T4065 11132-11140 NN denotes response
T4066 11141-11143 TO denotes to
T4067 11144-11151 NNP denotes LPS+ATP
T4068 11152-11154 IN denotes at
T4069 11155-11156 CD denotes 6
T4070 11157-11162 NNS denotes hours
T4071 11163-11164 -LRB- denotes (
T4072 11164-11168 NNS denotes data
T4073 11169-11172 RB denotes not
T4074 11173-11178 VBN denotes shown
T4075 11178-11179 -RRB- denotes )
T4076 11179-11180 . denotes .
T4077 11181-11192 NN denotes Stimulation
T4078 11193-11195 IN denotes of
T4079 11196-11201 NNS denotes PBMCs
T4080 11202-11206 IN denotes with
T4081 11207-11209 NNP denotes P.
T4082 11210-11220 NN denotes aeruginosa
T4083 11221-11225 WDT denotes that
T4084 11226-11231 VBZ denotes lacks
T4085 11232-11236 NNP denotes exsA
T4086 11237-11238 -LRB- denotes (
T4087 11238-11247 NNP denotes PAO1ΔexsA
T4088 11247-11248 -RRB- denotes )
T4089 11248-11249 , denotes ,
T4090 11250-11251 DT denotes a
T4091 11252-11255 JJ denotes key
T4092 11256-11265 NN denotes regulator
T4093 11266-11268 IN denotes of
T4094 11269-11273 NN denotes type
T4095 11274-11277 NNP denotes III
T4096 11278-11287 NN denotes secretion
T4097 11287-11288 , denotes ,
T4098 11289-11297 VBD denotes produced
T4099 11298-11308 JJ denotes three-fold
T4100 11309-11313 RBR denotes less
T4101 11314-11319 JJ denotes IL-1β
T4102 11320-11328 VBN denotes compared
T4103 11329-11331 TO denotes to
T4104 11332-11335 DT denotes the
T4105 11336-11344 JJ denotes parental
T4106 11345-11349 NNP denotes PAO1
T4107 11350-11356 NN denotes strain
T4108 11357-11359 IN denotes by
T4109 11360-11362 CD denotes 24
T4110 11363-11368 NNS denotes hours
T4111 11369-11370 -LRB- denotes (
T4112 11370-11374 NNP denotes Fig.
T4113 11375-11377 CD denotes 5a
T4114 11377-11378 -RRB- denotes )
T4115 11378-11379 . denotes .
T4116 11380-11392 JJ denotes Inflammasome
T4117 11393-11404 NN denotes stimulation
T4118 11405-11412 IN denotes without
T4119 11413-11420 VBG denotes priming
T4120 11421-11424 VBD denotes did
T4121 11425-11428 RB denotes not
T4122 11429-11435 VB denotes result
T4123 11436-11438 IN denotes in
T4124 11439-11442 DT denotes any
T4125 11443-11448 JJ denotes IL-1β
T4126 11449-11459 NN denotes production
T4127 11460-11462 IN denotes in
T4128 11463-11469 CC denotes either
T4129 11470-11472 NNP denotes CF
T4130 11473-11475 CC denotes or
T4131 11476-11483 NN denotes control
T4132 11484-11489 NNS denotes PBMCs
T4133 11489-11490 . denotes .
T4134 11491-11499 JJ denotes Contrary
T4135 11500-11502 TO denotes to
T4136 11503-11506 PRP$ denotes our
T4137 11507-11517 NN denotes hypothesis
T4138 11517-11518 , denotes ,
T4139 11519-11524 DT denotes these
T4140 11525-11532 NNS denotes results
T4141 11533-11541 VBP denotes indicate
T4142 11542-11546 IN denotes that
T4143 11547-11552 NNS denotes PBMCs
T4144 11553-11557 IN denotes from
T4145 11558-11560 NNP denotes CF
T4146 11561-11569 NNS denotes patients
T4147 11570-11572 VBP denotes do
T4148 11573-11576 RB denotes not
T4149 11577-11584 VB denotes display
T4150 11585-11594 VBN denotes increased
T4151 11595-11605 NN denotes production
T4152 11606-11608 IN denotes of
T4153 11609-11614 NN denotes IL-1β
T4154 11615-11617 CC denotes or
T4155 11618-11622 NN denotes IL-8
T4156 11623-11627 IN denotes with
T4157 11628-11640 JJ denotes inflammasome
T4158 11641-11651 NN denotes activation
T4159 11652-11655 CC denotes nor
T4160 11656-11658 VBP denotes do
T4161 11659-11663 PRP denotes they
T4162 11664-11671 VBP denotes suggest
T4163 11672-11675 DT denotes any
T4164 11676-11685 VBN denotes increased
T4165 11686-11691 NN denotes basal
T4166 11692-11694 CC denotes or
T4167 11695-11702 JJ denotes induced
T4168 11703-11708 NN denotes NF-κB
T4169 11709-11717 NN denotes activity
T4170 11717-11718 . denotes .
T4171 11719-11724 DT denotes These
T4172 11725-11732 NNS denotes results
T4173 11733-11736 VBP denotes are
T4174 11737-11747 JJ denotes consistent
T4175 11748-11752 IN denotes with
T4176 11753-11756 PRP$ denotes our
T4177 11757-11768 NN denotes observation
T4178 11769-11773 IN denotes that
T4179 11774-11783 JJ denotes caspase-1
T4180 11784-11792 NN denotes activity
T4181 11793-11795 VBZ denotes is
T4182 11796-11799 RB denotes not
T4183 11800-11809 JJ denotes different
T4184 11810-11817 IN denotes between
T4185 11818-11820 NNP denotes CF
T4186 11821-11824 CC denotes and
T4187 11825-11832 VB denotes control
T4188 11833-11838 NNP denotes PBMCs
T4189 11839-11840 -LRB- denotes (
T4190 11840-11844 NNP denotes Fig.
T4191 11845-11846 CD denotes 4
T4192 11846-11847 -RRB- denotes )
T4193 11847-11848 . denotes .
T4695 12380-12385 JJ denotes NF-κB
T4696 12386-12396 NN denotes activation
T4697 12397-12399 VBZ denotes is
T4698 12400-12408 VBN denotes required
T4699 12409-12412 IN denotes for
T4700 12413-12418 NN denotes IL-1β
T4701 12419-12422 CC denotes and
T4702 12423-12427 NN denotes IL-8
T4703 12428-12437 NNS denotes responses
T4704 12438-12440 TO denotes to
T4705 12441-12443 NNP denotes P.
T4706 12444-12454 NN denotes aeruginosa
T4707 12456-12458 PRP denotes We
T4708 12459-12468 VBD denotes confirmed
T4709 12469-12472 DT denotes the
T4710 12473-12483 NN denotes dependence
T4711 12484-12486 IN denotes of
T4712 12487-12499 JJ denotes PAO1-induced
T4713 12500-12505 NN denotes IL-1β
T4714 12506-12509 CC denotes and
T4715 12510-12514 NN denotes IL-8
T4716 12515-12525 NN denotes production
T4717 12526-12528 IN denotes on
T4718 12529-12534 JJ denotes NF-κB
T4719 12535-12545 NN denotes activation
T4720 12546-12551 VBG denotes using
T4721 12552-12557 CD denotes THP-1
T4722 12558-12563 NNS denotes cells
T4723 12564-12574 VBG denotes expressing
T4724 12575-12576 DT denotes a
T4725 12577-12585 NN denotes reporter
T4726 12586-12592 VBN denotes driven
T4727 12593-12595 IN denotes by
T4728 12596-12601 NNP denotes NF-κB
T4729 12602-12605 CC denotes and
T4730 12606-12610 NNP denotes AP-1
T4731 12611-12619 NN denotes response
T4732 12620-12628 NNS denotes elements
T4733 12628-12629 . denotes .
T4734 12630-12632 PRP denotes We
T4735 12633-12638 VBD denotes found
T4736 12639-12643 IN denotes that
T4737 12644-12655 NN denotes stimulation
T4738 12656-12658 IN denotes of
T4739 12659-12665 VBN denotes primed
T4740 12666-12671 CD denotes THP-1
T4741 12672-12680 NN denotes reporter
T4742 12681-12686 NNS denotes cells
T4743 12687-12691 IN denotes with
T4744 12692-12703 JJ denotes heat-killed
T4745 12704-12708 NNP denotes PAO1
T4746 12709-12717 VBD denotes produced
T4747 12718-12721 DT denotes the
T4748 12722-12729 JJS denotes highest
T4749 12730-12736 NNS denotes levels
T4750 12737-12739 IN denotes of
T4751 12740-12745 NNP denotes NF-κB
T4752 12745-12746 NNP denotes /
T4753 12746-12750 NNP denotes AP-1
T4754 12751-12761 NN denotes activation
T4755 12762-12763 -LRB- denotes (
T4756 12763-12767 NNP denotes Fig.
T4757 12768-12770 CD denotes 6a
T4758 12770-12771 -RRB- denotes )
T4759 12772-12775 CC denotes and
T4760 12776-12780 DT denotes this
T4761 12781-12791 VBN denotes correlated
T4762 12792-12796 IN denotes with
T4763 12797-12801 JJ denotes IL-8
T4764 12802-12811 NN denotes secretion
T4765 12812-12813 -LRB- denotes (
T4766 12813-12817 NNP denotes Fig.
T4767 12818-12820 CD denotes 6b
T4768 12820-12821 -RRB- denotes )
T4769 12822-12825 CC denotes but
T4770 12826-12836 JJ denotes negligible
T4771 12837-12844 NNS denotes amounts
T4772 12845-12847 IN denotes of
T4773 12848-12853 NN denotes IL-1β
T4774 12854-12858 VBD denotes were
T4775 12859-12867 VBN denotes secreted
T4776 12868-12869 -LRB- denotes (
T4777 12869-12873 NNP denotes Fig.
T4778 12874-12876 CD denotes 6c
T4779 12876-12877 -RRB- denotes )
T4780 12877-12878 . denotes .
T4781 12879-12890 NN denotes Stimulation
T4782 12891-12893 IN denotes of
T4783 12894-12900 VBN denotes primed
T4784 12901-12906 CD denotes THP-1
T4785 12907-12915 NN denotes reporter
T4786 12916-12921 NNS denotes cells
T4787 12922-12926 IN denotes with
T4788 12927-12931 JJ denotes live
T4789 12932-12936 NN denotes PAO1
T4790 12937-12940 VBD denotes did
T4791 12941-12944 RB denotes not
T4792 12945-12958 RB denotes significantly
T4793 12959-12967 VB denotes increase
T4794 12968-12973 JJ denotes NF-κB
T4795 12973-12974 NN denotes /
T4796 12974-12978 NN denotes AP-1
T4797 12979-12987 NN denotes activity
T4798 12988-12989 -LRB- denotes (
T4799 12989-12993 NNP denotes Fig.
T4800 12994-12996 CD denotes 6a
T4801 12996-12997 -RRB- denotes )
T4802 12998-13000 CC denotes or
T4803 13001-13005 JJ denotes IL-8
T4804 13006-13015 NN denotes secretion
T4805 13016-13017 -LRB- denotes (
T4806 13017-13021 NNP denotes Fig.
T4807 13022-13024 CD denotes 6b
T4808 13024-13025 -RRB- denotes )
T4809 13026-13030 IN denotes over
T4810 13031-13038 VBG denotes priming
T4811 13039-13044 RB denotes alone
T4812 13044-13045 . denotes .
T4813 13046-13053 RB denotes However
T4814 13053-13054 , denotes ,
T4815 13055-13060 JJ denotes IL-1β
T4816 13061-13071 NN denotes production
T4817 13072-13075 VBD denotes was
T4818 13076-13083 RB denotes greatly
T4819 13084-13093 VBN denotes augmented
T4820 13094-13098 IN denotes over
T4821 13099-13105 VBN denotes primed
T4822 13106-13111 NNS denotes cells
T4823 13112-13122 VBN denotes stimulated
T4824 13123-13127 IN denotes with
T4825 13128-13139 JJ denotes heat-killed
T4826 13140-13144 CD denotes PAO1
T4827 13145-13147 CC denotes or
T4828 13148-13156 JJ denotes unprimed
T4829 13157-13162 NNS denotes cells
T4830 13163-13173 VBN denotes stimulated
T4831 13174-13178 IN denotes with
T4832 13179-13183 JJ denotes live
T4833 13184-13188 NNP denotes PAO1
T4834 13189-13190 -LRB- denotes (
T4835 13190-13194 NNP denotes Fig.
T4836 13195-13197 CD denotes 6c
T4837 13197-13198 -RRB- denotes )
T4838 13198-13199 . denotes .
T4839 13200-13204 DT denotes This
T4840 13205-13214 VBD denotes confirmed
T4841 13215-13219 IN denotes that
T4842 13220-13225 JJ denotes NF-κB
T4843 13226-13236 NN denotes activation
T4844 13237-13242 RB denotes alone
T4845 13243-13245 VBZ denotes is
T4846 13246-13249 RB denotes not
T4847 13250-13260 JJ denotes sufficient
T4848 13261-13264 IN denotes for
T4849 13265-13272 JJ denotes maximal
T4850 13273-13278 JJ denotes IL-1β
T4851 13279-13288 NN denotes secretion
T4852 13288-13289 , denotes ,
T4853 13290-13293 CC denotes but
T4854 13294-13303 VBD denotes increased
T4855 13304-13311 NN denotes priming
T4856 13312-13314 IN denotes of
T4857 13315-13320 NNP denotes NF-κB
T4858 13321-13323 VBZ denotes is
T4859 13324-13331 JJ denotes capable
T4860 13332-13334 IN denotes of
T4861 13335-13345 VBG denotes augmenting
T4862 13346-13351 JJ denotes IL-1β
T4863 13352-13362 NN denotes production
T4864 13363-13366 CC denotes and
T4865 13367-13376 NN denotes secretion
T4866 13377-13381 IN denotes upon
T4867 13382-13394 JJ denotes inflammasome
T4868 13395-13406 NN denotes stimulation
T4869 13406-13407 . denotes .
T4870 13408-13418 NN denotes Dependency
T4871 13419-13421 IN denotes of
T4872 13422-13427 DT denotes these
T4873 13428-13437 NNS denotes responses
T4874 13438-13440 IN denotes on
T4875 13441-13446 NN denotes NF-κB
T4876 13447-13450 VBD denotes was
T4877 13451-13460 VBN denotes confirmed
T4878 13461-13463 IN denotes by
T4879 13464-13477 JJ denotes pharmacologic
T4880 13478-13488 NN denotes inhibition
T4881 13489-13491 IN denotes of
T4882 13492-13497 JJ denotes NF-κB
T4883 13498-13503 VBG denotes using
T4884 13504-13507 DT denotes the
T4885 13508-13518 JJ denotes Bay11-7082
T4886 13519-13528 NN denotes inhibitor
T4887 13529-13531 IN denotes of
T4888 13532-13536 NNP denotes IκBα
T4889 13537-13552 NN denotes phosphorylation
T4890 13552-13553 , denotes ,
T4891 13554-13559 WDT denotes which
T4892 13560-13573 RB denotes significantly
T4893 13574-13581 VBD denotes reduced
T4894 13582-13587 NNP denotes NF-κB
T4895 13587-13588 NNP denotes /
T4896 13588-13592 NNP denotes AP-1
T4897 13593-13603 NN denotes activation
T4898 13604-13605 -LRB- denotes (
T4899 13605-13606 NNP denotes P
T4900 13606-13607 CD denotes <
T4901 13607-13612 CD denotes 0.001
T4902 13612-13613 -RRB- denotes )
T4903 13614-13615 -LRB- denotes (
T4904 13615-13619 NNP denotes Fig.
T4905 13620-13622 CD denotes 6d
T4906 13622-13623 -RRB- denotes )
T4907 13624-13627 CC denotes and
T4908 13628-13631 DT denotes the
T4909 13632-13642 JJ denotes subsequent
T4910 13643-13653 NN denotes production
T4911 13654-13656 IN denotes of
T4912 13657-13661 NNP denotes IL-8
T4913 13662-13663 -LRB- denotes (
T4914 13663-13664 NNP denotes P
T4915 13664-13665 CD denotes <
T4916 13665-13669 CD denotes 0.01
T4917 13669-13670 -RRB- denotes )
T4918 13671-13672 -LRB- denotes (
T4919 13672-13676 NNP denotes Fig.
T4920 13677-13679 CD denotes 6e
T4921 13679-13680 -RRB- denotes )
T4922 13681-13684 CC denotes and
T4923 13685-13690 NNP denotes IL-1β
T4924 13691-13692 -LRB- denotes (
T4925 13692-13693 NNP denotes P
T4926 13693-13694 CD denotes <
T4927 13694-13699 CD denotes 0.001
T4928 13699-13700 -RRB- denotes )
T4929 13701-13702 -LRB- denotes (
T4930 13702-13706 NNP denotes Fig.
T4931 13707-13709 CD denotes 6f
T4932 13709-13710 -RRB- denotes )
T4933 13711-13713 IN denotes in
T4934 13714-13722 NN denotes response
T4935 13723-13725 TO denotes to
T4936 13726-13730 DT denotes both
T4937 13731-13742 JJ denotes heat-killed
T4938 13743-13746 CC denotes and
T4939 13747-13751 JJ denotes live
T4940 13752-13756 NN denotes PAO1
T4941 13756-13757 . denotes .
T4942 13758-13763 DT denotes These
T4943 13764-13771 NNS denotes results
T4944 13772-13776 VBD denotes were
T4945 13777-13781 RB denotes also
T4946 13782-13790 VBN denotes verified
T4947 13791-13793 IN denotes in
T4948 13794-13796 NNP denotes CF
T4949 13797-13800 CC denotes and
T4950 13801-13808 NN denotes control
T4951 13809-13814 NNS denotes PBMCs
T4952 13815-13818 IN denotes for
T4953 13819-13823 DT denotes each
T4954 13824-13836 JJ denotes inflammasome
T4955 13837-13845 VBN denotes examined
T4956 13846-13847 -LRB- denotes (
T4957 13847-13848 NNP denotes P
T4958 13848-13849 CD denotes <
T4959 13849-13854 CD denotes 0.001
T4960 13854-13855 -RRB- denotes )
T4961 13856-13857 -LRB- denotes (
T4962 13857-13861 NNP denotes Fig.
T4963 13862-13864 CD denotes 6g
T4964 13865-13866 NN denotes h
T4965 13866-13867 -RRB- denotes )
T4966 13867-13868 . denotes .
T4967 13869-13876 JJ denotes Overall
T4968 13877-13882 DT denotes these
T4969 13883-13890 NNS denotes results
T4970 13891-13898 VBP denotes confirm
T4971 13899-13903 IN denotes that
T4972 13904-13909 NNP denotes NF-κB
T4973 13910-13912 VBZ denotes is
T4974 13913-13915 DT denotes an
T4975 13916-13925 JJ denotes important
T4976 13926-13935 NN denotes modulator
T4977 13936-13938 IN denotes of
T4978 13939-13944 JJ denotes IL-1β
T4979 13945-13955 NN denotes production
T4980 13956-13959 CC denotes and
T4981 13960-13964 IN denotes that
T4982 13965-13974 VBN denotes increased
T4983 13975-13985 NN denotes activation
T4984 13986-13988 IN denotes of
T4985 13989-13994 JJ denotes NF-κB
T4986 13995-14003 NNS denotes augments
T4987 14004-14025 JJ denotes inflammasome-mediated
T4988 14026-14036 NN denotes production
T4989 14037-14039 IN denotes of
T4990 14040-14045 NN denotes IL-1β
T4991 14045-14046 . denotes .
T5326 15230-15240 JJ denotes Bay11-7082
T5327 15241-15249 NNS denotes inhibits
T5328 15250-15259 JJ denotes pro-IL-1β
T5329 15260-15270 NN denotes production
T5330 15271-15273 IN denotes in
T5331 15274-15282 NN denotes response
T5332 15283-15285 TO denotes to
T5333 15286-15288 NNP denotes P.
T5334 15289-15299 NN denotes aeruginosa
T5335 15301-15303 IN denotes In
T5336 15304-15312 NN denotes addition
T5337 15313-15315 TO denotes to
T5338 15316-15326 NN denotes inhibition
T5339 15327-15329 IN denotes of
T5340 15330-15335 JJ denotes NF-κB
T5341 15336-15344 NN denotes activity
T5342 15344-15345 , denotes ,
T5343 15346-15356 NN denotes Bay11-7082
T5344 15357-15360 MD denotes can
T5345 15361-15365 RB denotes also
T5346 15366-15374 RB denotes directly
T5347 15375-15382 VB denotes inhibit
T5348 15383-15386 DT denotes the
T5349 15387-15392 NNP denotes NLRP3
T5350 15393-15405 JJ denotes inflammasome
T5351 15406-15407 NNP denotes [
T5352 15407-15409 CD denotes 38
T5353 15409-15410 NNP denotes ]
T5354 15410-15411 . denotes .
T5355 15412-15414 TO denotes To
T5356 15415-15423 VB denotes validate
T5357 15424-15427 PRP$ denotes its
T5358 15428-15431 NN denotes use
T5359 15432-15434 IN denotes in
T5360 15435-15439 DT denotes this
T5361 15440-15445 NN denotes study
T5362 15446-15448 IN denotes as
T5363 15449-15451 DT denotes an
T5364 15452-15457 JJ denotes NF-κB
T5365 15458-15467 NN denotes inhibitor
T5366 15467-15468 , denotes ,
T5367 15469-15476 JJ denotes western
T5368 15477-15482 NNS denotes blots
T5369 15483-15486 IN denotes for
T5370 15487-15496 NN denotes pro-IL-1β
T5371 15497-15501 VBD denotes were
T5372 15502-15511 VBN denotes performed
T5373 15512-15521 IN denotes alongside
T5374 15522-15532 NN denotes inhibition
T5375 15533-15535 IN denotes of
T5376 15536-15540 NNP denotes CFTR
T5377 15541-15549 NN denotes activity
T5378 15550-15552 IN denotes by
T5379 15553-15563 NNP denotes CFTRinh172
T5380 15564-15566 IN denotes in
T5381 15567-15575 NN denotes response
T5382 15576-15578 TO denotes to
T5383 15579-15583 CD denotes PAO1
T5384 15584-15586 IN denotes at
T5385 15587-15588 CD denotes 4
T5386 15589-15594 NNS denotes hours
T5387 15595-15600 IN denotes after
T5388 15601-15612 NN denotes stimulation
T5389 15613-15614 -LRB- denotes (
T5390 15614-15618 NNP denotes Fig.
T5391 15619-15621 CD denotes 7a
T5392 15621-15622 -RRB- denotes )
T5393 15622-15623 . denotes .
T5394 15624-15627 PRP$ denotes Our
T5395 15628-15635 NNS denotes results
T5396 15636-15644 VBP denotes indicate
T5397 15645-15649 IN denotes that
T5398 15650-15660 JJ denotes Bay11-7082
T5399 15661-15669 VBZ denotes prevents
T5400 15670-15680 NN denotes production
T5401 15681-15683 IN denotes of
T5402 15684-15693 JJ denotes pro-IL-1β
T5403 15694-15701 IN denotes whereas
T5404 15702-15712 NNP denotes CFTRinh172
T5405 15713-15717 VBZ denotes does
T5406 15718-15721 RB denotes not
T5407 15722-15726 VB denotes seem
T5408 15727-15729 TO denotes to
T5409 15730-15736 VB denotes affect
T5410 15737-15739 PRP denotes it
T5411 15739-15740 . denotes .
T5412 15741-15745 DT denotes This
T5413 15746-15749 VBD denotes was
T5414 15750-15757 JJ denotes further
T5415 15758-15770 VBN denotes corroborated
T5416 15771-15773 IN denotes by
T5417 15774-15777 DT denotes the
T5418 15778-15785 NN denotes ability
T5419 15786-15796 NN denotes Bay11-7082
T5420 15797-15799 TO denotes to
T5421 15800-15807 VB denotes inhibit
T5422 15808-15812 NNP denotes IκBα
T5423 15813-15824 NN denotes degradation
T5424 15825-15827 IN denotes at
T5425 15828-15831 CD denotes 0.5
T5426 15831-15832 , denotes ,
T5427 15833-15834 CD denotes 1
T5428 15834-15835 , denotes ,
T5429 15836-15839 CC denotes and
T5430 15840-15843 CD denotes 1.5
T5431 15844-15849 NNS denotes hours
T5432 15850-15854 NN denotes post
T5433 15855-15859 CD denotes PAO1
T5434 15860-15871 NN denotes stimulation
T5435 15872-15873 -LRB- denotes (
T5436 15873-15877 NNP denotes Fig.
T5437 15878-15880 CD denotes 7b
T5438 15880-15881 -RRB- denotes )
T5439 15881-15882 . denotes .
T5639 16297-16307 NN denotes Disruption
T5640 16308-16310 IN denotes of
T5641 16311-16315 NNP denotes CFTR
T5642 16316-16324 NN denotes activity
T5643 16325-16329 VBZ denotes does
T5644 16330-16333 RB denotes not
T5645 16334-16342 VB denotes increase
T5646 16343-16348 JJ denotes IL-1β
T5647 16349-16359 NN denotes production
T5648 16360-16362 IN denotes in
T5649 16363-16368 NNP denotes PBMCs
T5650 16369-16372 CC denotes and
T5651 16373-16378 NNP denotes THP-1
T5652 16379-16384 NNS denotes cells
T5653 16385-16386 DT denotes A
T5654 16387-16395 JJ denotes previous
T5655 16396-16401 NN denotes study
T5656 16402-16405 VBZ denotes has
T5657 16406-16415 VBN denotes indicated
T5658 16416-16417 DT denotes a
T5659 16418-16422 NN denotes role
T5660 16423-16426 IN denotes for
T5661 16427-16435 NN denotes chloride
T5662 16436-16439 NN denotes ion
T5663 16440-16453 NN denotes concentration
T5664 16454-16456 IN denotes in
T5665 16457-16468 NN denotes suppression
T5666 16469-16471 IN denotes of
T5667 16472-16477 CD denotes NLRP3
T5668 16478-16490 JJ denotes inflammasome
T5669 16491-16501 NN denotes activation
T5670 16502-16503 NNP denotes [
T5671 16503-16505 CD denotes 34
T5672 16505-16506 NNP denotes ]
T5673 16506-16507 . denotes .
T5674 16508-16510 TO denotes To
T5675 16511-16520 VB denotes determine
T5676 16521-16528 IN denotes whether
T5677 16529-16533 NNP denotes CFTR
T5678 16534-16545 NN denotes dysfunction
T5679 16546-16552 VBZ denotes alters
T5680 16553-16558 JJ denotes IL-1β
T5681 16559-16569 NN denotes production
T5682 16569-16570 , denotes ,
T5683 16571-16576 CD denotes THP-1
T5684 16577-16582 NNS denotes cells
T5685 16583-16586 CC denotes and
T5686 16587-16592 NNS denotes PBMCs
T5687 16593-16597 IN denotes from
T5688 16598-16600 NNP denotes CF
T5689 16601-16609 NNS denotes patients
T5690 16610-16613 CC denotes and
T5691 16614-16621 JJ denotes healthy
T5692 16622-16630 NNS denotes controls
T5693 16631-16635 VBD denotes were
T5694 16636-16643 VBN denotes treated
T5695 16644-16648 IN denotes with
T5696 16649-16652 DT denotes the
T5697 16653-16657 NNP denotes CFTR
T5698 16658-16667 NN denotes inhibitor
T5699 16667-16668 , denotes ,
T5700 16669-16679 NNP denotes CFTRinh172
T5701 16679-16680 , denotes ,
T5702 16681-16686 RB denotes prior
T5703 16687-16689 TO denotes to
T5704 16690-16700 NN denotes simulation
T5705 16701-16705 IN denotes with
T5706 16706-16710 JJ denotes live
T5707 16711-16713 NNP denotes P.
T5708 16714-16724 NN denotes aeruginosa
T5709 16724-16725 . denotes .
T5710 16726-16735 NNP denotes Treatment
T5711 16736-16740 IN denotes with
T5712 16741-16751 NNP denotes CFTRinh172
T5713 16752-16755 VBD denotes did
T5714 16756-16759 RB denotes not
T5715 16760-16765 VB denotes alter
T5716 16766-16771 JJ denotes IL-1β
T5717 16772-16774 CC denotes or
T5718 16775-16779 JJ denotes IL-8
T5719 16780-16790 NN denotes production
T5720 16791-16793 IN denotes in
T5721 16794-16801 NN denotes control
T5722 16802-16810 NNS denotes subjects
T5723 16811-16813 CC denotes or
T5724 16814-16816 NN denotes CF
T5725 16817-16825 NNS denotes patients
T5726 16826-16827 -LRB- denotes (
T5727 16827-16831 NNP denotes Fig.
T5728 16832-16834 CD denotes 8a
T5729 16835-16836 NN denotes b
T5730 16836-16837 -RRB- denotes )
T5731 16837-16838 . denotes .
T5732 16839-16848 RB denotes Similarly
T5733 16848-16849 , denotes ,
T5734 16850-16855 JJ denotes IL-1β
T5735 16856-16866 NN denotes production
T5736 16867-16870 VBD denotes was
T5737 16871-16874 RB denotes not
T5738 16875-16884 JJ denotes different
T5739 16885-16887 IN denotes in
T5740 16888-16904 JJ denotes monocyte-derived
T5741 16905-16916 NNS denotes macrophages
T5742 16917-16919 CC denotes or
T5743 16920-16925 CD denotes THP-1
T5744 16926-16934 NN denotes reporter
T5745 16935-16940 NNS denotes cells
T5746 16941-16948 VBN denotes treated
T5747 16949-16953 IN denotes with
T5748 16954-16964 NNP denotes CFTRinh172
T5749 16965-16966 -LRB- denotes (
T5750 16966-16970 NNP denotes Fig.
T5751 16971-16973 CD denotes 8c
T5752 16974-16975 LS denotes d
T5753 16975-16976 -RRB- denotes )
T5754 16976-16977 . denotes .
T5755 16978-16982 NN denotes IL-8
T5756 16983-16984 -LRB- denotes (
T5757 16984-16988 NNP denotes Fig.
T5758 16989-16991 CD denotes 8e
T5759 16991-16992 -RRB- denotes )
T5760 16993-16996 CC denotes and
T5761 16997-17002 JJ denotes NF-κB
T5762 17003-17011 NN denotes activity
T5763 17012-17013 -LRB- denotes (
T5764 17013-17017 NNP denotes Fig.
T5765 17018-17020 CD denotes 8f
T5766 17020-17021 -RRB- denotes )
T5767 17022-17026 VBD denotes were
T5768 17027-17031 RB denotes also
T5769 17032-17041 JJ denotes unchanged
T5770 17042-17044 IN denotes in
T5771 17045-17063 JJ denotes CFTRinh172-treated
T5772 17064-17069 CD denotes THP-1
T5773 17070-17078 NN denotes reporter
T5774 17079-17084 NNS denotes cells
T5775 17084-17085 . denotes .
T7430 17820-17826 NNS denotes Levels
T7431 17827-17829 IN denotes of
T7432 17830-17835 NN denotes IL-1β
T7433 17836-17839 VBP denotes are
T7434 17840-17849 VBN denotes increased
T7435 17850-17852 IN denotes in
T7436 17853-17856 DT denotes the
T7437 17857-17861 NNP denotes BALF
T7438 17862-17864 IN denotes of
T7439 17865-17867 NNP denotes CF
T7440 17868-17876 NNS denotes patients
T7441 17877-17880 CC denotes but
T7442 17881-17884 DT denotes the
T7443 17885-17893 JJ denotes cellular
T7444 17894-17900 NN denotes source
T7445 17901-17903 IN denotes of
T7446 17904-17908 DT denotes this
T7447 17909-17917 NN denotes cytokine
T7448 17918-17921 CC denotes and
T7449 17922-17925 PRP$ denotes its
T7450 17926-17936 NN denotes production
T7451 17937-17939 IN denotes in
T7452 17940-17943 DT denotes the
T7453 17944-17951 NN denotes context
T7454 17952-17954 IN denotes of
T7455 17955-17963 JJ denotes targeted
T7456 17964-17976 JJ denotes inflammasome
T7457 17977-17987 NN denotes activation
T7458 17988-17991 VBP denotes are
T7459 17992-17997 RB denotes still
T7460 17998-18005 JJ denotes unclear
T7461 18005-18006 . denotes .
T7462 18007-18009 PRP denotes We
T7463 18010-18015 RB denotes first
T7464 18016-18023 VBD denotes studied
T7465 18024-18030 NN denotes airway
T7466 18031-18041 JJ denotes epithelial
T7467 18042-18047 NNS denotes cells
T7468 18048-18051 JJ denotes due
T7469 18052-18054 TO denotes to
T7470 18055-18060 PRP$ denotes their
T7471 18061-18065 NN denotes role
T7472 18066-18068 IN denotes in
T7473 18069-18076 NN denotes barrier
T7474 18077-18085 NN denotes function
T7475 18085-18086 , denotes ,
T7476 18087-18096 NN denotes proximity
T7477 18097-18099 TO denotes to
T7478 18100-18109 NN denotes infection
T7479 18109-18110 , denotes ,
T7480 18111-18114 CC denotes and
T7481 18115-18122 NN denotes ability
T7482 18123-18125 TO denotes to
T7483 18126-18133 VB denotes produce
T7484 18134-18138 JJ denotes high
T7485 18139-18145 NNS denotes levels
T7486 18146-18148 IN denotes of
T7487 18149-18165 JJ denotes pro-inflammatory
T7488 18166-18175 NNS denotes cytokines
T7489 18175-18176 . denotes .
T7490 18177-18184 RB denotes However
T7491 18184-18185 , denotes ,
T7492 18186-18188 PRP denotes we
T7493 18189-18194 VBD denotes found
T7494 18195-18199 IN denotes that
T7495 18200-18209 JJ denotes bronchial
T7496 18210-18220 JJ denotes epithelial
T7497 18221-18226 NNS denotes cells
T7498 18227-18229 VBP denotes do
T7499 18230-18233 RB denotes not
T7500 18234-18241 VB denotes produce
T7501 18242-18253 JJ denotes significant
T7502 18254-18261 NNS denotes amounts
T7503 18262-18264 IN denotes of
T7504 18265-18270 NN denotes IL-1β
T7505 18271-18274 CC denotes and
T7506 18275-18277 VB denotes do
T7507 18278-18281 RB denotes not
T7508 18282-18286 VB denotes show
T7509 18287-18288 DT denotes a
T7510 18289-18300 JJ denotes significant
T7511 18301-18309 NN denotes increase
T7512 18310-18312 IN denotes in
T7513 18313-18322 JJ denotes caspase-1
T7514 18323-18333 NN denotes activation
T7515 18334-18336 IN denotes in
T7516 18337-18345 NN denotes response
T7517 18346-18348 TO denotes to
T7518 18349-18353 CD denotes PAO1
T7519 18354-18357 CC denotes and
T7520 18358-18365 NNP denotes LPS+ATP
T7521 18365-18366 , denotes ,
T7522 18367-18369 IN denotes in
T7523 18370-18380 NN denotes comparison
T7524 18381-18383 TO denotes to
T7525 18384-18397 JJ denotes hematopoeitic
T7526 18398-18409 NN denotes mononuclear
T7527 18410-18415 NNS denotes cells
T7528 18415-18416 . denotes .
T7529 18417-18442 JJ denotes Hematopoeitically-derived
T7530 18443-18448 NNS denotes cells
T7531 18448-18449 , denotes ,
T7532 18450-18454 JJ denotes such
T7533 18455-18457 IN denotes as
T7534 18458-18467 NNS denotes monocytes
T7535 18468-18471 CC denotes and
T7536 18472-18483 NNS denotes macrophages
T7537 18483-18484 , denotes ,
T7538 18485-18491 VBP denotes appear
T7539 18492-18494 TO denotes to
T7540 18495-18497 VB denotes be
T7541 18498-18499 DT denotes a
T7542 18500-18509 JJ denotes principal
T7543 18510-18516 NN denotes source
T7544 18517-18519 IN denotes of
T7545 18520-18525 NN denotes IL-1β
T7546 18525-18526 . denotes .
T7547 18527-18531 NNP denotes CFTR
T7548 18532-18534 VBZ denotes is
T7549 18535-18544 VBN denotes expressed
T7550 18545-18547 IN denotes in
T7551 18548-18556 JJ denotes alveolar
T7552 18557-18568 NNS denotes macrophages
T7553 18569-18570 NNP denotes [
T7554 18570-18572 CD denotes 32
T7555 18572-18573 NNP denotes ]
T7556 18573-18574 , denotes ,
T7557 18575-18576 NNP denotes [
T7558 18576-18578 CD denotes 39
T7559 18578-18579 NNP denotes ]
T7560 18580-18582 RB denotes as
T7561 18583-18587 RB denotes well
T7562 18588-18590 IN denotes as
T7563 18591-18593 IN denotes in
T7564 18594-18599 NNP denotes PBMCs
T7565 18600-18602 IN denotes at
T7566 18603-18607 DT denotes both
T7567 18608-18611 DT denotes the
T7568 18612-18616 NNP denotes mRNA
T7569 18617-18620 CC denotes and
T7570 18621-18628 NN denotes protein
T7571 18629-18634 NN denotes level
T7572 18635-18636 NNP denotes [
T7573 18636-18638 CD denotes 40
T7574 18638-18639 NNP denotes ]
T7575 18639-18640 , denotes ,
T7576 18641-18642 NNP denotes [
T7577 18642-18644 CD denotes 41
T7578 18644-18645 NNP denotes ]
T7579 18645-18646 , denotes ,
T7580 18647-18650 CC denotes and
T7581 18651-18654 PRP$ denotes its
T7582 18655-18659 NN denotes loss
T7583 18660-18662 VBZ denotes is
T7584 18663-18673 RB denotes frequently
T7585 18674-18684 VBN denotes associated
T7586 18685-18689 IN denotes with
T7587 18690-18692 DT denotes an
T7588 18693-18702 JJ denotes augmented
T7589 18703-18715 JJ denotes inflammatory
T7590 18716-18725 NN denotes phenotype
T7591 18725-18726 . denotes .
T7592 18727-18734 IN denotes Despite
T7593 18735-18738 PRP$ denotes our
T7594 18739-18747 NNS denotes findings
T7595 18748-18758 VBG denotes indicating
T7596 18759-18763 IN denotes that
T7597 18764-18773 JJ denotes bronchial
T7598 18774-18784 JJ denotes epithelial
T7599 18785-18790 NNS denotes cells
T7600 18791-18795 WRB denotes when
T7601 18796-18801 VBN denotes grown
T7602 18802-18804 IN denotes in
T7603 18805-18810 NN denotes vitro
T7604 18811-18814 VBP denotes are
T7605 18815-18823 JJ denotes unlikely
T7606 18824-18826 TO denotes to
T7607 18827-18829 VB denotes be
T7608 18830-18843 RB denotes significantly
T7609 18844-18852 VBN denotes involved
T7610 18853-18855 IN denotes in
T7611 18856-18859 DT denotes the
T7612 18860-18866 JJ denotes direct
T7613 18867-18877 NN denotes production
T7614 18878-18880 IN denotes of
T7615 18881-18886 NNP denotes IL-1β
T7616 18887-18888 -LRB- denotes (
T7617 18888-18892 NNP denotes Fig.
T7618 18893-18895 CD denotes 2a
T7619 18896-18897 LS denotes d
T7620 18897-18898 -RRB- denotes )
T7621 18898-18899 , denotes ,
T7622 18900-18906 NNS denotes others
T7623 18907-18911 VBP denotes have
T7624 18912-18917 VBN denotes shown
T7625 18918-18923 PRP$ denotes their
T7626 18924-18932 NN denotes capacity
T7627 18933-18935 TO denotes to
T7628 18936-18943 VB denotes respond
T7629 18944-18946 TO denotes to
T7630 18947-18955 JJ denotes alveolar
T7631 18956-18974 JJ denotes macrophage-derived
T7632 18975-18980 NN denotes IL-1β
T7633 18981-18984 CC denotes and
T7634 18985-18987 TO denotes to
T7635 18988-18995 VB denotes amplify
T7636 18996-18999 DT denotes the
T7637 19000-19012 JJ denotes inflammatory
T7638 19013-19021 NN denotes response
T7639 19022-19029 IN denotes through
T7640 19030-19033 DT denotes the
T7641 19034-19043 NN denotes induction
T7642 19044-19046 IN denotes of
T7643 19047-19057 NNS denotes chemokines
T7644 19058-19061 CC denotes and
T7645 19062-19073 NN denotes recruitment
T7646 19074-19076 IN denotes of
T7647 19077-19089 JJ denotes inflammatory
T7648 19090-19098 NN denotes effector
T7649 19099-19104 NNS denotes cells
T7650 19105-19106 NNP denotes [
T7651 19106-19108 CD denotes 42
T7652 19108-19109 NNP denotes ]
T7653 19109-19110 . denotes .
T7654 19111-19115 DT denotes This
T7655 19116-19127 NN denotes interaction
T7656 19128-19131 MD denotes may
T7657 19132-19142 VB denotes constitute
T7658 19143-19144 DT denotes a
T7659 19145-19153 JJ denotes critical
T7660 19154-19163 NN denotes component
T7661 19164-19166 TO denotes to
T7662 19167-19176 JJ denotes effective
T7663 19177-19181 NN denotes host
T7664 19182-19189 NN denotes defense
T7665 19190-19193 CC denotes and
T7666 19194-19205 VBG denotes diminishing
T7667 19206-19209 DT denotes the
T7668 19210-19218 NN denotes capacity
T7669 19219-19221 IN denotes of
T7670 19222-19226 NN denotes host
T7671 19227-19232 NNS denotes cells
T7672 19233-19235 TO denotes to
T7673 19236-19243 VB denotes respond
T7674 19244-19246 TO denotes to
T7675 19247-19252 NNP denotes IL-1β
T7676 19253-19256 MD denotes may
T7677 19257-19262 VB denotes leave
T7678 19263-19266 DT denotes the
T7679 19267-19271 NN denotes host
T7680 19272-19283 JJ denotes susceptible
T7681 19284-19286 TO denotes to
T7682 19287-19297 NNS denotes infections
T7683 19298-19300 IN denotes by
T7684 19301-19310 NNS denotes pathogens
T7685 19311-19315 JJ denotes such
T7686 19316-19318 IN denotes as
T7687 19319-19321 NNP denotes P.
T7688 19322-19332 NN denotes aeruginosa
T7689 19333-19334 NNP denotes [
T7690 19334-19336 CD denotes 43
T7691 19336-19337 NNP denotes ]
T7692 19337-19338 . denotes .
T7693 19339-19349 RB denotes Conversely
T7694 19349-19350 , denotes ,
T7695 19351-19365 NN denotes overproduction
T7696 19366-19368 IN denotes of
T7697 19369-19374 NN denotes IL-1β
T7698 19375-19378 MD denotes can
T7699 19379-19383 RB denotes also
T7700 19384-19388 VB denotes play
T7701 19389-19390 DT denotes a
T7702 19391-19394 JJ denotes key
T7703 19395-19399 NN denotes role
T7704 19400-19402 IN denotes in
T7705 19403-19410 JJ denotes chronic
T7706 19411-19423 JJ denotes inflammatory
T7707 19424-19433 NNS denotes responses
T7708 19434-19437 CC denotes and
T7709 19438-19443 NN denotes cause
T7710 19444-19450 NN denotes damage
T7711 19451-19453 TO denotes to
T7712 19454-19457 DT denotes the
T7713 19458-19462 NN denotes lung
T7714 19463-19473 NN denotes parenchyma
T7715 19474-19475 NNP denotes [
T7716 19475-19477 CD denotes 44
T7717 19477-19478 NNP denotes ]
T7718 19478-19479 , denotes ,
T7719 19480-19481 NNP denotes [
T7720 19481-19483 CD denotes 45
T7721 19483-19484 NNP denotes ]
T7722 19484-19485 . denotes .
T7723 19486-19494 IN denotes Although
T7724 19495-19497 PRP denotes we
T7725 19498-19510 VBD denotes hypothesized
T7726 19511-19515 IN denotes that
T7727 19516-19518 NNP denotes CF
T7728 19519-19524 NNS denotes cells
T7729 19525-19530 MD denotes would
T7730 19531-19538 VB denotes secrete
T7731 19539-19548 JJ denotes increased
T7732 19549-19556 NNS denotes amounts
T7733 19557-19559 IN denotes of
T7734 19560-19565 NN denotes IL-1β
T7735 19565-19566 , denotes ,
T7736 19567-19569 PRP denotes we
T7737 19570-19575 VBD denotes found
T7738 19576-19580 IN denotes that
T7739 19581-19586 JJ denotes IL-1β
T7740 19587-19597 NN denotes production
T7741 19598-19600 IN denotes in
T7742 19601-19603 NNP denotes CF
T7743 19604-19609 NNP denotes PBMCs
T7744 19610-19613 VBD denotes was
T7745 19614-19617 RB denotes not
T7746 19618-19627 VBN denotes increased
T7747 19628-19632 IN denotes upon
T7748 19633-19645 JJ denotes inflammasome
T7749 19646-19657 NN denotes stimulation
T7750 19658-19660 IN denotes as
T7751 19661-19669 VBN denotes compared
T7752 19670-19672 TO denotes to
T7753 19673-19681 NNS denotes controls
T7754 19682-19683 -LRB- denotes (
T7755 19683-19687 NNP denotes Fig.
T7756 19688-19690 CD denotes 5a
T7757 19691-19692 NN denotes b
T7758 19692-19693 -RRB- denotes )
T7759 19693-19694 . denotes .
T7760 19695-19699 DT denotes This
T7761 19700-19703 VBD denotes was
T7762 19704-19706 IN denotes in
T7763 19707-19715 NN denotes contrast
T7764 19716-19718 TO denotes to
T7765 19719-19720 DT denotes a
T7766 19721-19729 JJ denotes previous
T7767 19730-19735 NN denotes study
T7768 19736-19740 IN denotes from
T7769 19741-19744 PRP$ denotes our
T7770 19745-19750 NN denotes group
T7771 19750-19751 , denotes ,
T7772 19752-19757 WDT denotes which
T7773 19758-19764 VBD denotes showed
T7774 19765-19774 VBN denotes increased
T7775 19775-19780 JJ denotes IL-1β
T7776 19781-19791 NN denotes production
T7777 19792-19794 IN denotes by
T7778 19795-19797 NNP denotes CF
T7779 19798-19803 NNP denotes PBMCs
T7780 19804-19806 IN denotes in
T7781 19807-19815 NN denotes response
T7782 19816-19818 TO denotes to
T7783 19819-19822 NNP denotes LPS
T7784 19823-19824 NNP denotes [
T7785 19824-19826 CD denotes 46
T7786 19826-19827 NNP denotes ]
T7787 19827-19828 , denotes ,
T7788 19829-19837 IN denotes although
T7789 19838-19842 DT denotes this
T7790 19843-19853 NN denotes difference
T7791 19854-19857 MD denotes may
T7792 19858-19860 VB denotes be
T7793 19861-19870 VBN denotes accounted
T7794 19871-19874 IN denotes for
T7795 19875-19877 IN denotes by
T7796 19878-19887 JJ denotes technical
T7797 19888-19894 NNS denotes issues
T7798 19895-19897 IN denotes in
T7799 19898-19909 NN denotes stimulation
T7800 19910-19914 NN denotes time
T7801 19915-19918 CC denotes and
T7802 19919-19923 NN denotes dose
T7803 19923-19924 . denotes .
T7804 19925-19933 RB denotes Moreover
T7805 19933-19934 , denotes ,
T7806 19935-19940 JJ denotes IL-1β
T7807 19941-19951 NN denotes production
T7808 19952-19955 VBD denotes was
T7809 19956-19959 RB denotes not
T7810 19960-19969 VBN denotes increased
T7811 19970-19972 IN denotes in
T7812 19973-19975 NNP denotes CF
T7813 19976-19981 NNP denotes PBMCs
T7814 19982-19986 IN denotes with
T7815 19987-19999 JJ denotes inflammasome
T7816 20000-20011 NN denotes stimulation
T7817 20012-20017 RB denotes alone
T7818 20018-20020 RB denotes as
T7819 20021-20026 MD denotes would
T7820 20027-20029 VB denotes be
T7821 20030-20041 VBN denotes anticipated
T7822 20042-20044 IN denotes if
T7823 20045-20050 EX denotes there
T7824 20051-20055 VBD denotes were
T7825 20056-20061 JJ denotes basal
T7826 20062-20068 NNS denotes levels
T7827 20069-20071 IN denotes of
T7828 20072-20077 JJ denotes NF-κB
T7829 20078-20088 NN denotes activation
T7830 20088-20089 . denotes .
T7831 20090-20095 NNS denotes Cells
T7832 20096-20105 VBP denotes deficient
T7833 20106-20108 IN denotes in
T7834 20109-20113 NNP denotes CFTR
T7835 20114-20117 VBP denotes are
T7836 20118-20125 VBN denotes thought
T7837 20126-20128 TO denotes to
T7838 20129-20136 VB denotes exhibit
T7839 20137-20139 DT denotes an
T7840 20140-20149 VBN denotes increased
T7841 20150-20155 JJ denotes basal
T7842 20156-20161 NN denotes level
T7843 20162-20164 IN denotes of
T7844 20165-20170 JJ denotes NF-κB
T7845 20171-20179 NN denotes activity
T7846 20179-20180 , denotes ,
T7847 20181-20186 WDT denotes which
T7848 20187-20192 VBZ denotes leads
T7849 20193-20195 TO denotes to
T7850 20196-20205 VBN denotes increased
T7851 20206-20222 JJ denotes pro-inflammatory
T7852 20223-20231 NN denotes cytokine
T7853 20232-20242 NN denotes production
T7854 20243-20252 VBG denotes including
T7855 20253-20255 DT denotes an
T7856 20256-20265 JJ denotes increased
T7857 20266-20278 NN denotes availability
T7858 20279-20281 IN denotes of
T7859 20282-20291 NN denotes pro-IL-1β
T7860 20292-20295 IN denotes for
T7861 20296-20304 NN denotes cleavage
T7862 20305-20308 CC denotes and
T7863 20309-20318 NN denotes secretion
T7864 20318-20319 . denotes .
T7865 20320-20324 DT denotes This
T7866 20325-20338 NN denotes amplification
T7867 20339-20341 IN denotes of
T7868 20342-20347 JJ denotes IL-1β
T7869 20348-20357 NN denotes secretion
T7870 20358-20361 VBD denotes was
T7871 20362-20367 VBN denotes shown
T7872 20368-20370 IN denotes by
T7873 20371-20378 VBG denotes priming
T7874 20379-20384 CD denotes THP-1
T7875 20385-20394 NNS denotes monocytes
T7876 20395-20398 CC denotes and
T7877 20399-20404 NNS denotes PBMCs
T7878 20405-20409 IN denotes with
T7879 20410-20421 JJ denotes heat-killed
T7880 20422-20424 NNP denotes P.
T7881 20425-20435 NN denotes aeruginosa
T7882 20436-20438 CC denotes or
T7883 20439-20442 NNP denotes LPS
T7884 20443-20448 RB denotes prior
T7885 20449-20451 TO denotes to
T7886 20452-20463 NN denotes stimulation
T7887 20464-20468 IN denotes with
T7888 20469-20473 JJ denotes live
T7889 20474-20476 NNP denotes P.
T7890 20477-20487 NN denotes aeruginosa
T7891 20487-20488 . denotes .
T7892 20489-20493 DT denotes This
T7893 20494-20506 RB denotes dramatically
T7894 20507-20516 VBN denotes increased
T7895 20517-20522 JJ denotes IL-1β
T7896 20523-20532 NN denotes secretion
T7897 20533-20537 IN denotes over
T7898 20538-20549 NN denotes stimulation
T7899 20550-20554 IN denotes with
T7900 20555-20559 JJ denotes live
T7901 20560-20562 NNP denotes P.
T7902 20563-20573 NN denotes aeruginosa
T7903 20574-20581 IN denotes without
T7904 20582-20589 NN denotes priming
T7905 20590-20591 -LRB- denotes (
T7906 20591-20595 NNP denotes Fig.
T7907 20596-20598 CD denotes 6c
T7908 20598-20599 -RRB- denotes )
T7909 20599-20600 . denotes .
T7910 20601-20610 RB denotes Similarly
T7911 20610-20611 , denotes ,
T7912 20612-20614 IN denotes if
T7913 20615-20617 NNP denotes CF
T7914 20618-20623 NNP denotes PBMCs
T7915 20624-20633 VBD denotes expressed
T7916 20634-20643 VBN denotes increased
T7917 20644-20649 JJ denotes basal
T7918 20650-20655 NN denotes NF-κB
T7919 20656-20664 NN denotes activity
T7920 20664-20665 , denotes ,
T7921 20666-20671 EX denotes there
T7922 20672-20677 MD denotes would
T7923 20678-20680 VB denotes be
T7924 20681-20683 DT denotes an
T7925 20684-20692 NN denotes increase
T7926 20693-20695 IN denotes in
T7927 20696-20701 JJ denotes IL-1β
T7928 20702-20711 NN denotes secretion
T7929 20712-20714 IN denotes in
T7930 20715-20718 DT denotes the
T7931 20719-20726 NN denotes absence
T7932 20727-20729 IN denotes of
T7933 20730-20733 NNP denotes LPS
T7934 20734-20741 NN denotes priming
T7935 20741-20742 . denotes .
T7936 20743-20750 RB denotes However
T7937 20750-20751 , denotes ,
T7938 20752-20754 DT denotes no
T7939 20755-20763 NN denotes increase
T7940 20764-20766 IN denotes in
T7941 20767-20772 NN denotes IL-1β
T7942 20773-20776 VBD denotes was
T7943 20777-20785 VBN denotes observed
T7944 20786-20791 IN denotes under
T7945 20792-20797 NN denotes basal
T7946 20798-20800 CC denotes or
T7947 20801-20807 VBN denotes primed
T7948 20808-20818 NNS denotes conditions
T7949 20818-20819 . denotes .
T7950 20820-20827 NNP denotes Studies
T7951 20828-20841 VBG denotes investigating
T7952 20842-20845 DT denotes the
T7953 20846-20856 NN denotes production
T7954 20857-20859 IN denotes of
T7955 20860-20865 NN denotes IL-1β
T7956 20866-20870 VBP denotes have
T7957 20871-20875 VBN denotes been
T7958 20876-20884 RB denotes somewhat
T7959 20885-20897 JJ denotes inconsistent
T7960 20897-20898 . denotes .
T7961 20899-20900 DT denotes A
T7962 20901-20906 NN denotes study
T7963 20907-20909 IN denotes by
T7964 20910-20919 NNP denotes Reininger
T7965 20920-20922 FW denotes et
T7966 20923-20926 FW denotes al.
T7967 20927-20928 NNP denotes [
T7968 20928-20930 CD denotes 43
T7969 20930-20931 NNP denotes ]
T7970 20932-20940 VBD denotes provided
T7971 20941-20949 NN denotes evidence
T7972 20950-20954 IN denotes that
T7973 20955-20960 JJ denotes human
T7974 20961-20970 JJ denotes bronchial
T7975 20971-20981 JJ denotes epithelial
T7976 20982-20987 NNS denotes cells
T7977 20988-20998 VBG denotes possessing
T7978 20999-21002 DT denotes the
T7979 21003-21008 NNP denotes ΔF508
T7980 21009-21013 NNP denotes CFTR
T7981 21014-21022 NN denotes mutation
T7982 21023-21026 VBD denotes had
T7983 21027-21028 DT denotes a
T7984 21029-21037 RB denotes slightly
T7985 21038-21045 VBN denotes reduced
T7986 21046-21054 NN denotes capacity
T7987 21055-21057 TO denotes to
T7988 21058-21065 VB denotes produce
T7989 21066-21071 JJ denotes IL-1β
T7990 21072-21075 CC denotes and
T7991 21076-21082 VBD denotes lacked
T7992 21083-21086 DT denotes the
T7993 21087-21094 NN denotes ability
T7994 21095-21097 TO denotes to
T7995 21098-21104 VB denotes induce
T7996 21105-21107 DT denotes an
T7997 21108-21113 JJ denotes early
T7998 21114-21119 NN denotes NF-κB
T7999 21120-21130 NN denotes activation
T8000 21131-21133 IN denotes in
T8001 21134-21142 NN denotes response
T8002 21143-21145 TO denotes to
T8003 21146-21148 NNP denotes P.
T8004 21149-21159 NN denotes aeruginosa
T8005 21159-21160 . denotes .
T8006 21161-21171 RB denotes Conversely
T8007 21171-21172 , denotes ,
T8008 21173-21174 DT denotes a
T8009 21175-21180 NN denotes study
T8010 21181-21183 IN denotes by
T8011 21184-21192 NNP denotes Kotrange
T8012 21193-21195 FW denotes et
T8013 21196-21199 FW denotes al.
T8014 21200-21201 NNP denotes [
T8015 21201-21203 CD denotes 31
T8016 21203-21204 NNP denotes ]
T8017 21205-21210 VBD denotes found
T8018 21211-21215 IN denotes that
T8019 21216-21222 JJ denotes murine
T8020 21223-21227 NN denotes bone
T8021 21228-21242 JJ denotes marrow-derived
T8022 21243-21254 NNS denotes macrophages
T8023 21255-21265 VBG denotes expressing
T8024 21266-21276 NN denotes ΔF508-CFTR
T8025 21277-21285 VBD denotes produced
T8026 21286-21295 VBN denotes increased
T8027 21296-21303 NNS denotes amounts
T8028 21304-21306 IN denotes of
T8029 21307-21312 NN denotes IL-1β
T8030 21313-21317 WRB denotes when
T8031 21318-21326 VBN denotes compared
T8032 21327-21329 TO denotes to
T8033 21330-21341 NNS denotes macrophages
T8034 21342-21352 VBG denotes expressing
T8035 21353-21359 JJ denotes normal
T8036 21360-21364 NNP denotes CFTR
T8037 21365-21367 IN denotes in
T8038 21368-21376 NN denotes response
T8039 21377-21379 TO denotes to
T8040 21380-21392 NNP denotes Burkholderia
T8041 21393-21404 NN denotes cenocepacia
T8042 21405-21410 NN denotes K56-2
T8043 21410-21411 . denotes .
T8044 21412-21415 DT denotes The
T8045 21416-21431 NN denotes differentiation
T8046 21432-21434 IN denotes of
T8047 21435-21444 NNS denotes monocytes
T8048 21445-21449 IN denotes into
T8049 21450-21461 NNS denotes macrophages
T8050 21462-21465 MD denotes may
T8051 21466-21472 RB denotes partly
T8052 21473-21480 VB denotes account
T8053 21481-21484 IN denotes for
T8054 21485-21488 DT denotes the
T8055 21489-21500 NNS denotes differences
T8056 21501-21509 VBD denotes observed
T8057 21510-21514 IN denotes with
T8058 21515-21518 DT denotes the
T8059 21519-21524 NN denotes study
T8060 21525-21527 IN denotes by
T8061 21528-21536 NNP denotes Kotrange
T8062 21536-21537 . denotes .
T8063 21538-21559 JJ denotes Inflammasome-mediated
T8064 21560-21565 JJ denotes IL-1β
T8065 21566-21576 NN denotes production
T8066 21577-21579 IN denotes by
T8067 21580-21589 NNS denotes monocytes
T8068 21590-21593 CC denotes and
T8069 21594-21599 NNS denotes PBMCs
T8070 21600-21604 VBZ denotes does
T8071 21605-21611 VB denotes differ
T8072 21612-21616 IN denotes from
T8073 21617-21628 NNS denotes macrophages
T8074 21629-21630 NNP denotes [
T8075 21630-21632 CD denotes 47
T8076 21632-21633 NNP denotes ]
T8077 21633-21634 , denotes ,
T8078 21635-21636 NNP denotes [
T8079 21636-21638 CD denotes 48
T8080 21638-21639 NNP denotes ]
T8081 21639-21640 , denotes ,
T8082 21641-21642 NNP denotes [
T8083 21642-21644 CD denotes 49
T8084 21644-21645 NNP denotes ]
T8085 21646-21649 CC denotes and
T8086 21650-21661 NNS denotes macrophages
T8087 21662-21665 VBP denotes are
T8088 21666-21671 VBN denotes found
T8089 21672-21674 TO denotes to
T8090 21675-21679 VB denotes have
T8091 21680-21686 JJR denotes higher
T8092 21687-21697 NN denotes expression
T8093 21698-21700 IN denotes of
T8094 21701-21705 NNP denotes CFTR
T8095 21706-21710 IN denotes over
T8096 21711-21720 NNS denotes monocytes
T8097 21721-21722 NNP denotes [
T8098 21722-21724 CD denotes 50
T8099 21724-21725 NNP denotes ]
T8100 21725-21726 . denotes .
T8101 21727-21734 RB denotes However
T8102 21734-21735 , denotes ,
T8103 21736-21738 IN denotes as
T8104 21739-21748 NNS denotes monocytes
T8105 21749-21752 CC denotes and
T8106 21753-21758 JJ denotes other
T8107 21759-21764 NNS denotes PBMCs
T8108 21765-21772 VBP denotes express
T8109 21773-21777 NNP denotes CFTR
T8110 21778-21779 NNP denotes [
T8111 21779-21781 CD denotes 50
T8112 21781-21782 NNP denotes ]
T8113 21782-21783 , denotes ,
T8114 21784-21785 NNP denotes [
T8115 21785-21787 CD denotes 51
T8116 21787-21788 NNP denotes ]
T8117 21789-21792 CC denotes and
T8118 21793-21800 VB denotes produce
T8119 21801-21806 JJ denotes large
T8120 21807-21814 NNS denotes amounts
T8121 21815-21817 IN denotes of
T8122 21818-21823 NN denotes IL-1β
T8123 21823-21824 , denotes ,
T8124 21825-21829 PRP denotes they
T8125 21830-21833 VBP denotes are
T8126 21834-21842 JJ denotes adequate
T8127 21843-21849 NNS denotes models
T8128 21850-21852 TO denotes to
T8129 21853-21860 VB denotes examine
T8130 21861-21864 DT denotes the
T8131 21865-21872 NNS denotes effects
T8132 21873-21875 IN denotes of
T8133 21876-21880 NNP denotes CFTR
T8134 21881-21889 NN denotes function
T8135 21890-21892 IN denotes on
T8136 21893-21898 JJ denotes IL-1β
T8137 21899-21909 NN denotes production
T8138 21909-21910 . denotes .
T8139 21911-21916 JJ denotes Other
T8140 21917-21930 JJ denotes hematopoeitic
T8141 21931-21936 NNS denotes cells
T8142 21937-21940 MD denotes may
T8143 21941-21945 RB denotes also
T8144 21946-21956 VB denotes contribute
T8145 21957-21959 TO denotes to
T8146 21960-21965 JJ denotes IL-1β
T8147 21966-21976 NN denotes production
T8148 21976-21977 . denotes .
T8149 21978-21981 IN denotes For
T8150 21982-21989 NN denotes example
T8151 21989-21990 , denotes ,
T8152 21991-22001 NN denotes neutrophil
T8153 22002-22008 NNS denotes counts
T8154 22009-22012 MD denotes can
T8155 22013-22015 VB denotes be
T8156 22016-22029 RB denotes significantly
T8157 22030-22039 VBN denotes increased
T8158 22040-22042 IN denotes in
T8159 22043-22046 DT denotes the
T8160 22047-22052 NNS denotes lungs
T8161 22053-22055 IN denotes of
T8162 22056-22058 NNP denotes CF
T8163 22059-22067 NNS denotes patients
T8164 22068-22069 NNP denotes [
T8165 22069-22071 CD denotes 52
T8166 22071-22072 NNP denotes ]
T8167 22072-22073 , denotes ,
T8168 22074-22075 NNP denotes [
T8169 22075-22077 CD denotes 53
T8170 22077-22078 NNP denotes ]
T8171 22078-22079 , denotes ,
T8172 22080-22081 NNP denotes [
T8173 22081-22083 CD denotes 54
T8174 22083-22084 NNP denotes ]
T8175 22085-22088 CC denotes and
T8176 22089-22092 MD denotes may
T8177 22093-22100 VB denotes produce
T8178 22101-22107 JJ denotes mature
T8179 22108-22113 NN denotes IL-1β
T8180 22114-22121 IN denotes through
T8181 22122-22131 JJ denotes caspase-1
T8182 22132-22143 JJ denotes independent
T8183 22144-22154 NNS denotes mechanisms
T8184 22155-22156 NNP denotes [
T8185 22156-22158 CD denotes 55
T8186 22158-22159 NNP denotes ]
T8187 22159-22160 . denotes .
T8188 22161-22164 DT denotes The
T8189 22165-22169 NN denotes role
T8190 22170-22172 IN denotes of
T8191 22173-22178 JJ denotes NF-κB
T8192 22179-22189 NN denotes activation
T8193 22190-22192 IN denotes in
T8194 22193-22205 JJ denotes inflammasome
T8195 22206-22216 NN denotes activation
T8196 22217-22220 CC denotes and
T8197 22221-22226 JJ denotes IL-1β
T8198 22227-22236 NN denotes secretion
T8199 22237-22239 VBZ denotes is
T8200 22240-22243 RB denotes not
T8201 22244-22259 JJ denotes straightforward
T8202 22259-22260 . denotes .
T8203 22261-22268 NNS denotes Studies
T8204 22269-22273 VBP denotes have
T8205 22274-22282 VBN denotes revealed
T8206 22283-22285 DT denotes an
T8207 22286-22295 JJ denotes essential
T8208 22296-22300 NN denotes role
T8209 22301-22304 IN denotes for
T8210 22305-22310 JJ denotes NF-κB
T8211 22311-22321 NN denotes activation
T8212 22322-22324 IN denotes in
T8213 22325-22328 DT denotes the
T8214 22329-22339 NN denotes production
T8215 22340-22342 IN denotes of
T8216 22343-22352 NN denotes pro-IL-1β
T8217 22353-22356 CC denotes and
T8218 22357-22369 JJ denotes inflammasome
T8219 22370-22380 NNS denotes components
T8220 22381-22385 JJ denotes such
T8221 22386-22388 IN denotes as
T8222 22389-22394 NNP denotes NLRP3
T8223 22395-22396 NNP denotes [
T8224 22396-22398 CD denotes 22
T8225 22398-22399 NNP denotes ]
T8226 22399-22400 , denotes ,
T8227 22401-22402 NNP denotes [
T8228 22402-22404 CD denotes 23
T8229 22404-22405 NNP denotes ]
T8230 22405-22406 . denotes .
T8231 22407-22409 IN denotes In
T8232 22410-22418 NN denotes contrast
T8233 22418-22419 , denotes ,
T8234 22420-22428 NN denotes deletion
T8235 22429-22431 IN denotes of
T8236 22432-22436 NNP denotes IKKβ
T8237 22436-22437 , denotes ,
T8238 22438-22439 DT denotes a
T8239 22440-22446 NN denotes kinase
T8240 22447-22456 JJ denotes essential
T8241 22457-22459 IN denotes in
T8242 22460-22465 JJ denotes NF-κB
T8243 22466-22476 NN denotes activation
T8244 22476-22477 , denotes ,
T8245 22478-22487 VBZ denotes increases
T8246 22488-22493 JJ denotes IL-1β
T8247 22494-22503 NN denotes secretion
T8248 22504-22506 IN denotes in
T8249 22507-22513 JJ denotes murine
T8250 22514-22525 NNS denotes macrophages
T8251 22526-22527 NNP denotes [
T8252 22527-22529 CD denotes 56
T8253 22529-22530 NNP denotes ]
T8254 22530-22531 , denotes ,
T8255 22532-22533 NNP denotes [
T8256 22533-22535 CD denotes 57
T8257 22535-22536 NNP denotes ]
T8258 22537-22540 CC denotes and
T8259 22541-22553 VBZ denotes demonstrates
T8260 22554-22555 DT denotes a
T8261 22556-22560 JJ denotes dual
T8262 22561-22565 NN denotes role
T8263 22566-22569 IN denotes for
T8264 22570-22575 NN denotes NF-κB
T8265 22576-22578 IN denotes in
T8266 22579-22589 NN denotes regulation
T8267 22590-22592 IN denotes of
T8268 22593-22598 NN denotes IL-1β
T8269 22598-22599 . denotes .
T8270 22600-22602 TO denotes To
T8271 22603-22610 VB denotes address
T8272 22611-22615 DT denotes this
T8273 22616-22627 NN denotes uncertainty
T8274 22628-22630 IN denotes in
T8275 22631-22634 PRP$ denotes our
T8276 22635-22646 NNS denotes experiments
T8277 22647-22649 PRP denotes we
T8278 22650-22654 RB denotes also
T8279 22655-22665 VBD denotes quantified
T8280 22666-22670 NNP denotes IL-8
T8281 22670-22671 , denotes ,
T8282 22672-22674 DT denotes an
T8283 22675-22684 JJ denotes important
T8284 22685-22687 NNP denotes CF
T8285 22688-22696 NN denotes cytokine
T8286 22697-22700 CC denotes and
T8287 22701-22707 NN denotes marker
T8288 22708-22710 IN denotes of
T8289 22711-22716 JJ denotes NF-κB
T8290 22717-22727 NN denotes activation
T8291 22728-22729 NNP denotes [
T8292 22729-22731 CD denotes 58
T8293 22731-22732 NNP denotes ]
T8294 22732-22733 , denotes ,
T8295 22734-22737 CC denotes and
T8296 22738-22743 VBD denotes found
T8297 22744-22746 DT denotes no
T8298 22747-22758 NNS denotes differences
T8299 22759-22766 IN denotes between
T8300 22767-22769 NNP denotes CF
T8301 22770-22773 CC denotes and
T8302 22774-22781 VB denotes control
T8303 22782-22790 NNS denotes subjects
T8304 22790-22791 . denotes .
T8305 22792-22801 RB denotes Similarly
T8306 22801-22802 , denotes ,
T8307 22803-22809 NNS denotes levels
T8308 22810-22812 IN denotes of
T8309 22813-22826 JJ denotes intracellular
T8310 22827-22836 NN denotes pro-IL-1β
T8311 22837-22839 IN denotes in
T8312 22840-22845 CD denotes THP-1
T8313 22846-22851 NNS denotes cells
T8314 22852-22856 VBD denotes were
T8315 22857-22866 JJ denotes dependent
T8316 22867-22869 IN denotes on
T8317 22870-22875 JJ denotes NF-κB
T8318 22876-22884 NN denotes activity
T8319 22885-22888 CC denotes and
T8320 22889-22892 VBD denotes did
T8321 22893-22896 RB denotes not
T8322 22897-22905 VB denotes increase
T8323 22906-22910 IN denotes with
T8324 22911-22921 JJ denotes CFTRinh172
T8325 22922-22931 NN denotes treatment
T8326 22931-22932 . denotes .
T8327 22933-22943 JJ denotes Subsequent
T8328 22944-22953 NN denotes treatment
T8329 22954-22956 IN denotes of
T8330 22957-22962 CD denotes THP-1
T8331 22963-22968 NNS denotes cells
T8332 22969-22972 CC denotes and
T8333 22973-22978 NNS denotes PBMCs
T8334 22979-22983 IN denotes with
T8335 22984-22987 DT denotes the
T8336 22988-22993 JJ denotes NF-κB
T8337 22994-23003 NN denotes inhibitor
T8338 23004-23014 NN denotes Bay11-7082
T8339 23015-23028 RB denotes significantly
T8340 23029-23038 VBD denotes inhibited
T8341 23039-23043 DT denotes both
T8342 23044-23049 JJ denotes IL-1β
T8343 23050-23053 CC denotes and
T8344 23054-23058 JJ denotes IL-8
T8345 23059-23068 NN denotes secretion
T8346 23069-23070 -LRB- denotes (
T8347 23070-23074 NNP denotes Fig.
T8348 23075-23077 CD denotes 6d
T8349 23078-23079 NN denotes h
T8350 23079-23080 -RRB- denotes )
T8351 23080-23081 . denotes .
T8352 23082-23091 RB denotes Therefore
T8353 23091-23092 , denotes ,
T8354 23093-23096 DT denotes the
T8355 23097-23102 NNP denotes IL-1β
T8356 23103-23106 CC denotes and
T8357 23107-23111 NNP denotes IL-8
T8358 23112-23121 NNS denotes responses
T8359 23122-23130 VBD denotes observed
T8360 23131-23135 VBD denotes were
T8361 23136-23140 DT denotes both
T8362 23141-23150 JJ denotes dependent
T8363 23151-23155 IN denotes upon
T8364 23156-23161 JJ denotes NF-κB
T8365 23162-23172 NN denotes activation
T8366 23172-23173 . denotes .
T8367 23174-23181 VBG denotes Priming
T8368 23182-23186 IN denotes with
T8369 23187-23198 JJ denotes heat-killed
T8370 23199-23201 NNP denotes P.
T8371 23202-23212 NN denotes aeruginosa
T8372 23212-23213 , denotes ,
T8373 23214-23218 IN denotes like
T8374 23219-23222 NNP denotes LPS
T8375 23222-23223 , denotes ,
T8376 23224-23226 VBZ denotes is
T8377 23227-23233 JJ denotes unable
T8378 23234-23236 TO denotes to
T8379 23237-23243 VB denotes induce
T8380 23244-23245 DT denotes a
T8381 23246-23252 JJ denotes strong
T8382 23253-23258 JJ denotes IL-1β
T8383 23259-23267 NN denotes response
T8384 23268-23270 IN denotes as
T8385 23271-23279 VBN denotes compared
T8386 23280-23282 TO denotes to
T8387 23283-23287 VB denotes live
T8388 23288-23290 NNP denotes P.
T8389 23291-23301 NN denotes aeruginosa
T8390 23302-23303 -LRB- denotes (
T8391 23303-23307 NNP denotes Fig.
T8392 23308-23310 CD denotes 6c
T8393 23310-23311 -RRB- denotes )
T8394 23311-23312 , denotes ,
T8395 23313-23316 CC denotes but
T8396 23317-23326 VBD denotes generated
T8397 23327-23334 JJR denotes greater
T8398 23335-23340 NNP denotes NF-κB
T8399 23340-23341 NNP denotes /
T8400 23341-23345 NNP denotes AP-1
T8401 23346-23356 NN denotes activation
T8402 23357-23358 -LRB- denotes (
T8403 23358-23362 NNP denotes Fig.
T8404 23363-23365 CD denotes 6a
T8405 23365-23366 -RRB- denotes )
T8406 23367-23370 CC denotes and
T8407 23371-23375 JJ denotes IL-8
T8408 23376-23385 NN denotes secretion
T8409 23386-23387 -LRB- denotes (
T8410 23387-23391 NNP denotes Fig.
T8411 23392-23394 CD denotes 6b
T8412 23394-23395 -RRB- denotes )
T8413 23396-23400 IN denotes than
T8414 23401-23405 JJ denotes live
T8415 23406-23414 NNS denotes bacteria
T8416 23415-23422 IN denotes despite
T8417 23423-23434 NN denotes stimulation
T8418 23435-23437 IN denotes at
T8419 23438-23448 JJ denotes equivalent
T8420 23449-23453 NNS denotes MOIs
T8421 23453-23454 . denotes .
T8422 23455-23459 DT denotes This
T8423 23460-23463 MD denotes may
T8424 23464-23466 VB denotes be
T8425 23467-23477 JJ denotes indicative
T8426 23478-23480 IN denotes of
T8427 23481-23484 DT denotes the
T8428 23485-23494 JJ denotes different
T8429 23495-23501 NN denotes degree
T8430 23502-23505 CC denotes and
T8431 23506-23513 NN denotes quality
T8432 23514-23516 IN denotes of
T8433 23517-23520 DT denotes the
T8434 23521-23533 JJ denotes inflammatory
T8435 23534-23542 NN denotes response
T8436 23543-23552 VBN denotes generated
T8437 23553-23555 IN denotes by
T8438 23556-23560 JJ denotes live
T8439 23561-23563 IN denotes as
T8440 23564-23571 VBN denotes opposed
T8441 23572-23574 TO denotes to
T8442 23575-23579 JJ denotes dead
T8443 23580-23588 NNS denotes bacteria
T8444 23589-23590 NNP denotes [
T8445 23590-23592 CD denotes 59
T8446 23592-23593 NNP denotes ]
T8447 23593-23594 . denotes .
T8448 23595-23604 JJ denotes Potential
T8449 23605-23617 NNS denotes shortcomings
T8450 23618-23620 IN denotes of
T8451 23621-23626 DT denotes these
T8452 23627-23638 NNS denotes experiments
T8453 23639-23646 VBP denotes include
T8454 23647-23650 PRP$ denotes its
T8455 23651-23666 NN denotes translatability
T8456 23667-23669 TO denotes to
T8457 23670-23674 NN denotes lung
T8458 23675-23682 NN denotes disease
T8459 23683-23686 CC denotes and
T8460 23687-23693 NNS denotes issues
T8461 23694-23701 VBN denotes related
T8462 23702-23704 TO denotes to
T8463 23705-23708 DT denotes the
T8464 23709-23724 NN denotes hypermutability
T8465 23725-23727 IN denotes of
T8466 23728-23730 NNP denotes P.
T8467 23731-23741 NN denotes aeruginosa
T8468 23742-23748 IN denotes during
T8469 23749-23752 DT denotes the
T8470 23753-23762 NN denotes evolution
T8471 23763-23765 IN denotes of
T8472 23766-23773 JJ denotes chronic
T8473 23774-23783 NN denotes infection
T8474 23783-23784 . denotes .
T8475 23785-23793 IN denotes Although
T8476 23794-23797 DT denotes the
T8477 23798-23807 NNS denotes responses
T8478 23808-23816 VBN denotes measured
T8479 23817-23819 IN denotes in
T8480 23820-23830 JJ denotes peripheral
T8481 23831-23836 NN denotes blood
T8482 23837-23842 NNS denotes cells
T8483 23843-23846 MD denotes may
T8484 23847-23850 RB denotes not
T8485 23851-23861 RB denotes completely
T8486 23862-23869 VB denotes reflect
T8487 23870-23873 DT denotes the
T8488 23874-23883 NNS denotes responses
T8489 23884-23893 VBG denotes occurring
T8490 23894-23896 IN denotes in
T8491 23897-23900 DT denotes the
T8492 23901-23903 NNP denotes CF
T8493 23904-23908 NN denotes lung
T8494 23908-23909 , denotes ,
T8495 23910-23915 NNS denotes PBMCs
T8496 23916-23920 VBP denotes have
T8497 23921-23922 DT denotes a
T8498 23923-23929 NN denotes number
T8499 23930-23932 IN denotes of
T8500 23933-23939 JJ denotes useful
T8501 23940-23950 NNS denotes advantages
T8502 23950-23951 : denotes :
T8503 23952-23953 -LRB- denotes (
T8504 23953-23954 FW denotes i
T8505 23954-23955 -RRB- denotes )
T8506 23956-23961 NNS denotes PBMCs
T8507 23962-23965 VBP denotes are
T8508 23966-23969 RB denotes not
T8509 23970-23977 JJ denotes subject
T8510 23978-23980 TO denotes to
T8511 23981-23992 NNS denotes alterations
T8512 23993-23997 WDT denotes that
T8513 23998-24001 MD denotes may
T8514 24002-24008 VB denotes emerge
T8515 24009-24013 IN denotes from
T8516 24014-24023 JJ denotes long-term
T8517 24024-24028 NN denotes cell
T8518 24029-24036 NN denotes culture
T8519 24036-24037 , denotes ,
T8520 24038-24045 VBG denotes cloning
T8521 24046-24049 CC denotes and
T8522 24050-24065 NN denotes immortalization
T8523 24065-24066 , denotes ,
T8524 24067-24070 CC denotes and
T8525 24071-24072 -LRB- denotes (
T8526 24072-24074 FW denotes ii
T8527 24074-24075 -RRB- denotes )
T8528 24076-24081 VBZ denotes PBMCs
T8529 24082-24089 VB denotes express
T8530 24090-24091 DT denotes a
T8531 24092-24097 JJ denotes large
T8532 24098-24108 NN denotes repertoire
T8533 24109-24111 IN denotes of
T8534 24112-24118 NN denotes innate
T8535 24119-24125 JJ denotes immune
T8536 24126-24135 NNS denotes receptors
T8537 24136-24139 CC denotes and
T8538 24140-24147 VB denotes secrete
T8539 24148-24149 DT denotes a
T8540 24150-24155 JJ denotes broad
T8541 24156-24161 NN denotes array
T8542 24162-24164 IN denotes of
T8543 24165-24174 NNS denotes cytokines
T8544 24175-24178 CC denotes and
T8545 24179-24189 NNS denotes chemokines
T8546 24190-24198 VBG denotes allowing
T8547 24199-24212 JJ denotes comprehensive
T8548 24213-24221 NN denotes analysis
T8549 24222-24224 IN denotes of
T8550 24225-24228 DT denotes the
T8551 24229-24239 NN denotes modulation
T8552 24240-24242 IN denotes of
T8553 24243-24255 JJ denotes inflammatory
T8554 24256-24265 NNS denotes responses
T8555 24266-24268 IN denotes by
T8556 24269-24273 NNP denotes CFTR
T8557 24273-24274 . denotes .
T8558 24275-24288 NN denotes Consideration
T8559 24289-24293 MD denotes must
T8560 24294-24298 RB denotes also
T8561 24299-24301 VB denotes be
T8562 24302-24307 VBN denotes given
T8563 24308-24310 TO denotes to
T8564 24311-24314 DT denotes the
T8565 24315-24321 NN denotes nature
T8566 24322-24324 IN denotes of
T8567 24325-24327 NNP denotes P.
T8568 24328-24338 NN denotes aeruginosa
T8569 24339-24348 NN denotes infection
T8570 24349-24352 CC denotes and
T8571 24353-24362 JJ denotes genotypic
T8572 24363-24370 NNS denotes changes
T8573 24371-24373 IN denotes in
T8574 24374-24376 NNP denotes P.
T8575 24377-24387 NN denotes aeruginosa
T8576 24388-24390 IN denotes as
T8577 24391-24400 NN denotes infection
T8578 24401-24411 VBZ denotes progresses
T8579 24411-24412 . denotes .
T8580 24413-24415 NNP denotes P.
T8581 24416-24426 NN denotes aeruginosa
T8582 24427-24435 VBZ denotes mediates
T8583 24436-24448 JJ denotes inflammasome
T8584 24449-24459 NN denotes activation
T8585 24460-24467 IN denotes through
T8586 24468-24471 PRP$ denotes its
T8587 24472-24476 NN denotes type
T8588 24477-24480 NNP denotes III
T8589 24481-24490 NN denotes secretion
T8590 24491-24497 NN denotes system
T8591 24498-24499 -LRB- denotes (
T8592 24499-24503 CD denotes T3SS
T8593 24503-24504 -RRB- denotes )
T8594 24505-24508 CC denotes and
T8595 24509-24512 DT denotes the
T8596 24513-24518 NNP denotes NLRC4
T8597 24519-24531 JJ denotes inflammasome
T8598 24532-24533 NNP denotes [
T8599 24533-24535 CD denotes 19
T8600 24535-24536 NNP denotes ]
T8601 24536-24537 , denotes ,
T8602 24538-24539 NNP denotes [
T8603 24539-24541 CD denotes 20
T8604 24541-24542 NNP denotes ]
T8605 24542-24543 . denotes .
T8606 24544-24551 RB denotes However
T8607 24551-24552 , denotes ,
T8608 24553-24559 NNS denotes clones
T8609 24560-24562 IN denotes of
T8610 24563-24565 NNP denotes P.
T8611 24566-24576 NN denotes aeruginosa
T8612 24577-24588 VBN denotes established
T8613 24589-24595 IN denotes during
T8614 24596-24603 JJ denotes chronic
T8615 24604-24613 NN denotes infection
T8616 24614-24617 MD denotes may
T8617 24618-24628 VB denotes accumulate
T8618 24629-24638 NNS denotes mutations
T8619 24639-24641 IN denotes in
T8620 24642-24651 NN denotes virulence
T8621 24652-24659 NNS denotes factors
T8622 24660-24664 JJ denotes such
T8623 24665-24667 IN denotes as
T8624 24668-24672 NNP denotes exsA
T8625 24673-24674 NNP denotes [
T8626 24674-24676 CD denotes 60
T8627 24676-24677 NNP denotes ]
T8628 24677-24678 . denotes .
T8629 24679-24681 IN denotes By
T8630 24682-24691 VBG denotes employing
T8631 24692-24693 DT denotes a
T8632 24694-24702 NN denotes deletion
T8633 24703-24709 JJ denotes mutant
T8634 24710-24712 IN denotes in
T8635 24713-24717 NNP denotes exsA
T8636 24717-24718 , denotes ,
T8637 24719-24722 DT denotes the
T8638 24723-24726 JJ denotes key
T8639 24727-24736 NN denotes regulator
T8640 24737-24739 IN denotes in
T8641 24740-24744 CD denotes T3SS
T8642 24745-24758 NN denotes transcription
T8643 24758-24759 , denotes ,
T8644 24760-24762 PRP denotes we
T8645 24763-24772 VBD denotes confirmed
T8646 24773-24777 IN denotes that
T8647 24778-24781 DT denotes the
T8648 24782-24786 CD denotes T3SS
T8649 24787-24789 VBZ denotes is
T8650 24790-24799 JJ denotes important
T8651 24800-24803 IN denotes for
T8652 24804-24809 JJ denotes IL-1β
T8653 24810-24819 NN denotes secretion
T8654 24820-24821 -LRB- denotes (
T8655 24821-24825 NNP denotes Fig.
T8656 24826-24828 CD denotes 5a
T8657 24829-24830 NN denotes b
T8658 24830-24831 -RRB- denotes )
T8659 24831-24832 , denotes ,
T8660 24833-24836 CC denotes and
T8661 24837-24841 IN denotes that
T8662 24842-24851 VBG denotes depending
T8663 24852-24854 IN denotes on
T8664 24855-24858 DT denotes the
T8665 24859-24869 NN denotes adaptation
T8666 24870-24872 IN denotes in
T8667 24873-24877 NN denotes type
T8668 24878-24881 NNP denotes III
T8669 24882-24891 NN denotes secretion
T8670 24891-24892 , denotes ,
T8671 24893-24896 DT denotes the
T8672 24897-24901 NN denotes host
T8673 24902-24907 NN denotes IL-1β
T8674 24908-24916 NN denotes response
T8675 24917-24920 MD denotes may
T8676 24921-24923 VB denotes be
T8677 24924-24926 RB denotes up
T8678 24927-24929 CC denotes or
T8679 24930-24943 VBN denotes downregulated
T8680 24944-24945 NNP denotes [
T8681 24945-24947 CD denotes 19
T8682 24947-24948 NNP denotes ]
T8683 24948-24949 , denotes ,
T8684 24950-24951 NNP denotes [
T8685 24951-24953 CD denotes 60
T8686 24953-24954 NNP denotes ]
T8687 24954-24955 , denotes ,
T8688 24956-24957 NNP denotes [
T8689 24957-24959 CD denotes 61
T8690 24959-24960 NNP denotes ]
T8691 24960-24961 . denotes .
T8692 24962-24964 IN denotes In
T8693 24965-24975 NN denotes conclusion
T8694 24975-24976 , denotes ,
T8695 24977-24980 PRP$ denotes our
T8696 24981-24985 NNS denotes data
T8697 24986-24989 VBP denotes are
T8698 24990-25000 JJ denotes consistent
T8699 25001-25005 IN denotes with
T8700 25006-25007 DT denotes a
T8701 25008-25012 NN denotes role
T8702 25013-25016 IN denotes for
T8703 25017-25030 JJ denotes hematopoietic
T8704 25031-25036 NNS denotes cells
T8705 25036-25037 , denotes ,
T8706 25038-25041 RB denotes not
T8707 25042-25048 VB denotes airway
T8708 25049-25059 JJ denotes epithelial
T8709 25060-25065 NNS denotes cells
T8710 25065-25066 , denotes ,
T8711 25067-25069 IN denotes as
T8712 25070-25073 DT denotes the
T8713 25074-25079 JJ denotes major
T8714 25080-25086 NN denotes source
T8715 25087-25089 IN denotes of
T8716 25090-25111 JJ denotes inflammasome-mediated
T8717 25112-25117 JJ denotes IL-1β
T8718 25118-25128 NN denotes production
T8719 25129-25131 IN denotes in
T8720 25132-25135 DT denotes the
T8721 25136-25141 NNS denotes lungs
T8722 25142-25144 IN denotes in
T8723 25145-25153 NN denotes response
T8724 25154-25156 TO denotes to
T8725 25157-25160 NNP denotes ATP
T8726 25161-25164 CC denotes and
T8727 25165-25167 NNP denotes P.
T8728 25168-25178 NN denotes aeruginosa
T8729 25178-25179 . denotes .
T8730 25180-25191 RB denotes Furthermore
T8731 25191-25192 , denotes ,
T8732 25193-25195 PRP denotes we
T8733 25196-25200 VBP denotes find
T8734 25201-25207 JJ denotes little
T8735 25208-25216 NN denotes evidence
T8736 25217-25219 TO denotes to
T8737 25220-25227 VB denotes support
T8738 25228-25230 DT denotes an
T8739 25231-25240 VBN denotes increased
T8740 25241-25246 NN denotes IL-1β
T8741 25247-25259 JJ denotes inflammatory
T8742 25260-25268 NN denotes response
T8743 25269-25271 TO denotes to
T8744 25272-25277 NNP denotes NF-κB
T8745 25277-25278 NNP denotes /
T8746 25278-25290 NNP denotes Inflammasome
T8747 25291-25302 NN denotes stimulation
T8748 25303-25305 IN denotes in
T8749 25306-25308 NNP denotes CF
T8750 25309-25317 NNS denotes patients
T8751 25317-25318 . denotes .
T8752 25319-25326 JJ denotes Further
T8753 25327-25334 NNS denotes studies
T8754 25335-25338 VBP denotes are
T8755 25339-25348 VBN denotes warranted
T8756 25349-25351 TO denotes to
T8757 25352-25361 VB denotes determine
T8758 25362-25364 IN denotes if
T8759 25365-25376 NNS denotes adaptations
T8760 25377-25379 IN denotes of
T8761 25380-25382 NNP denotes P.
T8762 25383-25393 NN denotes aeruginosa
T8763 25394-25400 IN denotes during
T8764 25401-25404 DT denotes the
T8765 25405-25411 NN denotes course
T8766 25412-25414 IN denotes of
T8767 25415-25422 JJ denotes chronic
T8768 25423-25427 NN denotes lung
T8769 25428-25437 NN denotes infection
T8770 25438-25444 NNS denotes alters
T8771 25445-25457 JJ denotes inflammasome
T8772 25458-25468 NN denotes activation
T8773 25468-25469 , denotes ,
T8774 25470-25473 CC denotes and
T8775 25474-25481 IN denotes whether
T8776 25482-25486 DT denotes this
T8777 25487-25490 MD denotes can
T8778 25491-25493 VB denotes be
T8779 25494-25504 VBN denotes correlated
T8780 25505-25509 IN denotes with
T8781 25510-25517 NN denotes disease
T8782 25518-25526 NN denotes severity
T8783 25527-25529 IN denotes in
T8784 25530-25533 NNP denotes CF.
T9373 25558-25564 NNS denotes Ethics
T9374 25565-25574 NNP denotes Statement
T9375 25575-25580 NNP denotes Blood
T9376 25581-25588 NNS denotes samples
T9377 25589-25593 VBD denotes were
T9378 25594-25602 VBN denotes obtained
T9379 25603-25607 IN denotes with
T9380 25608-25616 JJ denotes informed
T9381 25617-25624 VBN denotes written
T9382 25625-25632 NN denotes consent
T9383 25633-25637 IN denotes from
T9384 25638-25645 NN denotes control
T9385 25646-25654 NNS denotes subjects
T9386 25655-25658 CC denotes and
T9387 25659-25661 NNP denotes CF
T9388 25662-25670 NNS denotes patients
T9389 25671-25673 IN denotes at
T9390 25674-25677 DT denotes the
T9391 25678-25680 NNP denotes BC
T9392 25681-25689 NNP denotes Children
T9393 25689-25691 POS denotes 's
T9394 25692-25700 NN denotes Hospital
T9395 25700-25701 . denotes .
T9396 25702-25709 NNP denotes Consent
T9397 25710-25713 VBD denotes was
T9398 25714-25722 VBN denotes obtained
T9399 25723-25726 IN denotes for
T9400 25727-25735 NNS denotes children
T9401 25736-25738 IN denotes by
T9402 25739-25744 PRP$ denotes their
T9403 25745-25751 NN denotes parent
T9404 25752-25754 CC denotes or
T9405 25755-25760 JJ denotes legal
T9406 25761-25769 NN denotes guardian
T9407 25769-25770 . denotes .
T9408 25771-25779 NNS denotes Subjects
T9409 25780-25781 CD denotes 7
T9410 25782-25787 NNS denotes years
T9411 25788-25790 IN denotes of
T9412 25791-25794 NN denotes age
T9413 25795-25798 CC denotes and
T9414 25799-25804 JJR denotes older
T9415 25805-25809 VBD denotes were
T9416 25810-25818 VBN denotes required
T9417 25819-25821 TO denotes to
T9418 25822-25829 VB denotes provide
T9419 25830-25838 JJ denotes informed
T9420 25839-25845 NN denotes assent
T9421 25846-25848 RB denotes as
T9422 25849-25853 RB denotes well
T9423 25853-25854 . denotes .
T9424 25855-25864 NNS denotes Protocols
T9425 25865-25869 VBD denotes were
T9426 25870-25878 VBN denotes approved
T9427 25879-25881 IN denotes by
T9428 25882-25885 DT denotes the
T9429 25886-25894 NNP denotes Clinical
T9430 25895-25903 NNP denotes Research
T9431 25904-25910 NNP denotes Ethics
T9432 25911-25916 NNP denotes Board
T9433 25917-25918 -LRB- denotes (
T9434 25918-25927 NNP denotes H09-01192
T9435 25927-25928 -RRB- denotes )
T9436 25928-25929 . denotes .
T9881 25931-25935 NNP denotes Cell
T9882 25936-25943 NNP denotes Culture
T9883 25944-25946 NNP denotes CF
T9884 25947-25948 -LRB- denotes (
T9885 25948-25953 NNP denotes IB3-1
T9886 25954-25957 CC denotes and
T9887 25958-25964 NNP denotes CuFi-1
T9888 25964-25965 -RRB- denotes )
T9889 25966-25969 CC denotes and
T9890 25970-25977 NN denotes control
T9891 25978-25979 -LRB- denotes (
T9892 25979-25981 CD denotes S9
T9893 25982-25985 CC denotes and
T9894 25986-25992 NNP denotes NuLi-1
T9895 25992-25993 -RRB- denotes )
T9896 25994-25999 NNS denotes cells
T9897 26000-26004 VBD denotes were
T9898 26005-26013 VBN denotes obtained
T9899 26014-26018 IN denotes from
T9900 26019-26022 DT denotes the
T9901 26023-26031 JJ denotes American
T9902 26032-26036 NNP denotes Type
T9903 26037-26044 NNP denotes Culture
T9904 26045-26055 NNP denotes Collection
T9905 26055-26056 . denotes .
T9906 26057-26062 CD denotes IB3-1
T9907 26063-26068 NNS denotes cells
T9908 26069-26073 VBD denotes were
T9909 26074-26081 VBN denotes derived
T9910 26082-26086 IN denotes from
T9911 26087-26088 DT denotes a
T9912 26089-26096 JJ denotes patient
T9913 26097-26107 VBG denotes expressing
T9914 26108-26111 DT denotes the
T9915 26112-26117 NNP denotes ΔF508
T9916 26118-26121 CC denotes and
T9917 26122-26128 NNP denotes W1282X
T9918 26129-26138 NNS denotes mutations
T9919 26139-26142 CC denotes and
T9920 26143-26149 NN denotes CuFi-1
T9921 26150-26154 VBD denotes were
T9922 26155-26162 VBN denotes derived
T9923 26163-26167 IN denotes from
T9924 26168-26169 DT denotes a
T9925 26170-26175 NNP denotes ΔF508
T9926 26176-26186 JJ denotes homozygous
T9927 26187-26194 NN denotes patient
T9928 26194-26195 . denotes .
T9929 26196-26198 CD denotes S9
T9930 26199-26204 NNS denotes cells
T9931 26205-26208 VBP denotes are
T9932 26209-26214 JJ denotes IB3-1
T9933 26215-26220 NNS denotes cells
T9934 26221-26225 WDT denotes that
T9935 26226-26230 VBP denotes have
T9936 26231-26235 VBN denotes been
T9937 26236-26247 VBN denotes transfected
T9938 26248-26252 IN denotes with
T9939 26253-26257 NNP denotes CFTR
T9940 26258-26263 VBG denotes using
T9941 26264-26266 DT denotes an
T9942 26267-26283 JJ denotes adeno-associated
T9943 26284-26289 JJ denotes viral
T9944 26290-26296 NN denotes vector
T9945 26297-26300 CC denotes and
T9946 26301-26307 JJ denotes NuLi-1
T9947 26308-26313 NNS denotes cells
T9948 26314-26318 VBD denotes were
T9949 26319-26326 VBN denotes derived
T9950 26327-26331 IN denotes from
T9951 26332-26333 DT denotes a
T9952 26334-26341 JJ denotes patient
T9953 26342-26352 VBG denotes possessing
T9954 26353-26354 DT denotes a
T9955 26355-26364 JJ denotes wild-type
T9956 26365-26369 NNP denotes CFTR
T9957 26370-26378 NN denotes genotype
T9958 26378-26379 . denotes .
T9959 26380-26390 JJ denotes THP1-XBlue
T9960 26391-26396 NNS denotes cells
T9961 26397-26403 RB denotes stably
T9962 26404-26411 VBP denotes express
T9963 26412-26413 DT denotes a
T9964 26414-26422 JJ denotes secreted
T9965 26423-26432 JJ denotes embryonic
T9966 26433-26441 NN denotes alkaline
T9967 26442-26453 NN denotes phosphatase
T9968 26454-26455 -LRB- denotes (
T9969 26455-26459 NNP denotes SEAP
T9970 26459-26460 -RRB- denotes )
T9971 26461-26469 NN denotes reporter
T9972 26470-26479 JJ denotes inducible
T9973 26480-26482 IN denotes by
T9974 26483-26488 NNP denotes NF-κB
T9975 26489-26492 CC denotes and
T9976 26493-26497 NNP denotes AP-1
T9977 26498-26499 -LRB- denotes (
T9978 26499-26508 NNP denotes Invivogen
T9979 26508-26509 -RRB- denotes )
T9980 26509-26510 . denotes .
T9981 26511-26516 NNS denotes Cells
T9982 26517-26521 VBD denotes were
T9983 26522-26530 VBN denotes cultured
T9984 26531-26533 IN denotes as
T9985 26534-26545 VBN denotes recommended
T9986 26546-26548 IN denotes by
T9987 26549-26554 PRP$ denotes their
T9988 26555-26565 JJ denotes respective
T9989 26566-26575 NNS denotes suppliers
T9990 26576-26581 VBG denotes using
T9991 26582-26590 JJ denotes standard
T9992 26591-26600 NNS denotes protocols
T9993 26600-26601 . denotes .
T9994 26602-26604 CD denotes S9
T9995 26605-26608 CC denotes and
T9996 26609-26614 CD denotes IB3-1
T9997 26615-26620 NNS denotes cells
T9998 26621-26625 VBD denotes were
T9999 26626-26634 VBN denotes cultured
T10000 26635-26637 IN denotes in
T10001 26638-26643 JJ denotes basal
T10002 26644-26649 NN denotes LHC-8
T10003 26650-26651 -LRB- denotes (
T10004 26651-26661 NNP denotes Invitrogen
T10005 26661-26662 -RRB- denotes )
T10006 26663-26675 VBN denotes supplemented
T10007 26676-26680 IN denotes with
T10008 26681-26683 CD denotes 10
T10009 26683-26684 NN denotes %
T10010 26685-26686 -LRB- denotes (
T10011 26686-26689 FW denotes v/v
T10012 26689-26690 -RRB- denotes )
T10013 26691-26696 JJ denotes fetal
T10014 26697-26703 JJ denotes bovine
T10015 26704-26709 NN denotes serum
T10016 26710-26711 -LRB- denotes (
T10017 26711-26714 NNP denotes FBS
T10018 26714-26715 -RRB- denotes )
T10019 26715-26716 , denotes ,
T10020 26717-26718 CD denotes 2
T10021 26719-26721 NNP denotes mM
T10022 26722-26733 NNP denotes L-glutamine
T10023 26733-26734 , denotes ,
T10024 26735-26738 CC denotes and
T10025 26739-26740 CD denotes 1
T10026 26741-26743 NNP denotes mM
T10027 26744-26750 NN denotes sodium
T10028 26751-26759 NN denotes pyruvate
T10029 26759-26760 . denotes .
T10030 26761-26767 JJ denotes NuLi-1
T10031 26767-26768 NN denotes /
T10032 26768-26774 NN denotes CuFi-1
T10033 26775-26780 NNS denotes cells
T10034 26781-26785 VBD denotes were
T10035 26786-26794 VBN denotes cultured
T10036 26795-26797 IN denotes in
T10037 26798-26802 NNP denotes BEBM
T10038 26803-26813 JJ denotes serum-free
T10039 26814-26820 NN denotes medium
T10040 26821-26822 -LRB- denotes (
T10041 26822-26827 NNP denotes Lonza
T10042 26827-26828 -RRB- denotes )
T10043 26829-26833 IN denotes with
T10044 26834-26844 NN denotes supplement
T10045 26845-26851 NN denotes bullet
T10046 26852-26855 NN denotes kit
T10047 26856-26857 -LRB- denotes (
T10048 26857-26860 NNP denotes EGF
T10049 26860-26861 , denotes ,
T10050 26862-26876 NN denotes hydrocortisone
T10051 26876-26877 , denotes ,
T10052 26878-26884 JJ denotes bovine
T10053 26885-26894 JJ denotes pituitary
T10054 26895-26902 VB denotes extract
T10055 26902-26903 , denotes ,
T10056 26904-26915 NN denotes transferrin
T10057 26915-26916 , denotes ,
T10058 26917-26923 JJ denotes bovine
T10059 26924-26931 NN denotes insulin
T10060 26931-26932 , denotes ,
T10061 26933-26949 NN denotes triiodothyronine
T10062 26949-26950 , denotes ,
T10063 26951-26962 NN denotes epinephrine
T10064 26962-26963 , denotes ,
T10065 26964-26972 JJ denotes retinoic
T10066 26973-26977 NN denotes acid
T10067 26977-26978 -RRB- denotes )
T10068 26978-26979 , denotes ,
T10069 26980-26981 CD denotes 2
T10070 26982-26984 NNP denotes mM
T10071 26985-26996 NNP denotes L-glutamine
T10072 26996-26997 , denotes ,
T10073 26998-27001 CC denotes and
T10074 27002-27003 CD denotes 1
T10075 27004-27006 NNP denotes mM
T10076 27007-27013 NN denotes sodium
T10077 27014-27022 NN denotes pyruvate
T10078 27022-27023 . denotes .
T10079 27024-27029 NNS denotes PBMCs
T10080 27030-27034 IN denotes from
T10081 27035-27037 NNP denotes CF
T10082 27038-27046 NNS denotes patients
T10083 27047-27050 CC denotes and
T10084 27051-27059 NNS denotes controls
T10085 27060-27064 VBD denotes were
T10086 27065-27073 VBN denotes cultured
T10087 27074-27076 IN denotes in
T10088 27077-27086 NNP denotes RPMI-1640
T10089 27087-27088 -LRB- denotes (
T10090 27088-27095 NNP denotes Hyclone
T10091 27095-27096 -RRB- denotes )
T10092 27097-27109 VBN denotes supplemented
T10093 27110-27114 IN denotes with
T10094 27115-27117 CD denotes 10
T10095 27117-27118 NN denotes %
T10096 27119-27122 NNP denotes FBS
T10097 27122-27123 , denotes ,
T10098 27124-27125 CD denotes 2
T10099 27126-27128 NNP denotes mM
T10100 27129-27140 NNP denotes L-glutamine
T10101 27140-27141 , denotes ,
T10102 27142-27145 CC denotes and
T10103 27146-27147 CD denotes 1
T10104 27148-27150 NNP denotes mM
T10105 27151-27157 NN denotes sodium
T10106 27158-27166 NN denotes pyruvate
T10107 27167-27168 -LRB- denotes (
T10108 27168-27176 JJ denotes complete
T10109 27177-27181 NNP denotes RPMI
T10110 27181-27182 -RRB- denotes )
T10111 27182-27183 . denotes .
T10112 27184-27194 JJ denotes THP1-XBlue
T10113 27195-27200 NNS denotes cells
T10114 27201-27205 VBD denotes were
T10115 27206-27214 VBN denotes cultured
T10116 27215-27217 IN denotes in
T10117 27218-27226 JJ denotes complete
T10118 27227-27231 NNP denotes RPMI
T10119 27232-27236 IN denotes with
T10120 27237-27240 DT denotes the
T10121 27241-27249 NN denotes addition
T10122 27250-27252 IN denotes of
T10123 27253-27259 NN denotes zeocin
T10124 27260-27261 -LRB- denotes (
T10125 27261-27264 CD denotes 100
T10126 27265-27267 NN denotes µg
T10127 27267-27268 NN denotes /
T10128 27268-27270 NN denotes ml
T10129 27270-27271 -RRB- denotes )
T10130 27272-27274 TO denotes to
T10131 27275-27281 VB denotes select
T10132 27282-27285 IN denotes for
T10133 27286-27291 NNS denotes cells
T10134 27292-27302 VBG denotes expressing
T10135 27303-27306 DT denotes the
T10136 27307-27311 NNP denotes SEAP
T10137 27312-27317 NNP denotes NF-κB
T10138 27317-27318 NNP denotes /
T10139 27318-27322 NNP denotes AP-1
T10140 27323-27331 NN denotes reporter
T10141 27331-27332 . denotes .
T10142 27333-27338 RB denotes Prior
T10143 27339-27341 TO denotes to
T10144 27342-27353 NN denotes stimulation
T10145 27353-27354 , denotes ,
T10146 27355-27364 JJ denotes bronchial
T10147 27365-27375 JJ denotes epithelial
T10148 27376-27380 NN denotes cell
T10149 27381-27386 NNS denotes lines
T10150 27387-27391 VBD denotes were
T10151 27392-27398 VBN denotes plated
T10152 27399-27401 IN denotes in
T10153 27402-27408 JJ denotes coated
T10154 27409-27410 NNP denotes [
T10155 27410-27412 CD denotes 62
T10156 27412-27413 NNP denotes ]
T10157 27414-27421 JJ denotes 96-well
T10158 27422-27428 NNS denotes plates
T10159 27429-27430 -LRB- denotes (
T10160 27430-27432 NNP denotes BD
T10161 27433-27444 NNPS denotes Biosciences
T10162 27444-27445 -RRB- denotes )
T10163 27446-27448 IN denotes at
T10164 27449-27450 CD denotes 3
T10165 27450-27451 CD denotes ×
T10166 27451-27454 CD denotes 104
T10167 27455-27465 NN denotes cells/well
T10168 27466-27472 IN denotes unless
T10169 27473-27482 VBN denotes indicated
T10170 27482-27483 , denotes ,
T10171 27484-27487 CC denotes and
T10172 27488-27495 VBD denotes allowed
T10173 27496-27498 TO denotes to
T10174 27499-27505 VB denotes adhere
T10175 27506-27515 JJ denotes overnight
T10176 27515-27516 . denotes .
T10177 27517-27523 NNP denotes Plates
T10178 27524-27527 IN denotes for
T10179 27528-27530 NNP denotes S9
T10180 27531-27534 CC denotes and
T10181 27535-27540 NNP denotes IB3-1
T10182 27541-27553 NNS denotes stimulations
T10183 27554-27558 VBD denotes were
T10184 27559-27565 VBN denotes coated
T10185 27566-27568 IN denotes in
T10186 27569-27570 DT denotes a
T10187 27571-27578 NN denotes mixture
T10188 27579-27581 IN denotes of
T10189 27582-27588 JJ denotes bovine
T10190 27589-27594 NN denotes serum
T10191 27595-27602 NN denotes albumin
T10192 27603-27604 -LRB- denotes (
T10193 27604-27607 CD denotes 100
T10194 27608-27610 NN denotes µg
T10195 27610-27611 NN denotes /
T10196 27611-27613 NN denotes ml
T10197 27613-27614 -RRB- denotes )
T10198 27614-27615 , denotes ,
T10199 27616-27627 NN denotes fibronectin
T10200 27628-27629 -LRB- denotes (
T10201 27629-27631 CD denotes 10
T10202 27632-27634 NN denotes µg
T10203 27634-27635 NN denotes /
T10204 27635-27637 NN denotes ml
T10205 27637-27638 -RRB- denotes )
T10206 27638-27639 , denotes ,
T10207 27640-27643 CC denotes and
T10208 27644-27650 JJ denotes bovine
T10209 27651-27658 NN denotes collage
T10210 27659-27663 NN denotes type
T10211 27664-27665 PRP denotes I
T10212 27666-27667 -LRB- denotes (
T10213 27667-27669 CD denotes 30
T10214 27670-27672 NN denotes µg
T10215 27672-27673 NN denotes /
T10216 27673-27675 NN denotes ml
T10217 27675-27676 -RRB- denotes )
T10218 27677-27678 -LRB- denotes (
T10219 27678-27680 NNP denotes BD
T10220 27681-27692 NNPS denotes Biosciences
T10221 27692-27693 -RRB- denotes )
T10222 27693-27694 . denotes .
T10223 27695-27701 NNS denotes Plates
T10224 27702-27705 IN denotes for
T10225 27706-27712 NN denotes NuLi-1
T10226 27713-27716 CC denotes and
T10227 27717-27723 NN denotes CuFi-1
T10228 27724-27728 VBD denotes were
T10229 27729-27735 VBN denotes coated
T10230 27736-27740 IN denotes with
T10231 27741-27749 NN denotes collagen
T10232 27750-27754 NN denotes type
T10233 27755-27757 NNP denotes IV
T10234 27758-27759 -LRB- denotes (
T10235 27759-27761 CD denotes 60
T10236 27762-27764 NN denotes µg
T10237 27764-27765 NN denotes /
T10238 27765-27767 NN denotes ml
T10239 27767-27768 -RRB- denotes )
T10240 27769-27770 -LRB- denotes (
T10241 27770-27775 NNP denotes Sigma
T10242 27776-27783 NNP denotes Aldrich
T10243 27783-27784 -RRB- denotes )
T10244 27784-27785 . denotes .
T10245 27786-27791 NNS denotes PBMCs
T10246 27792-27796 VBD denotes were
T10247 27797-27803 VBN denotes plated
T10248 27804-27806 IN denotes in
T10249 27807-27814 JJ denotes 96-well
T10250 27815-27821 NNS denotes plates
T10251 27822-27824 IN denotes at
T10252 27825-27826 DT denotes a
T10253 27827-27834 NN denotes density
T10254 27835-27837 IN denotes of
T10255 27838-27841 CD denotes 1.5
T10256 27841-27842 CD denotes ×
T10257 27842-27845 CD denotes 105
T10258 27846-27856 NN denotes cells/well
T10259 27857-27859 IN denotes in
T10260 27860-27863 CD denotes 200
T10261 27864-27866 NN denotes µl
T10262 27867-27868 -LRB- denotes (
T10263 27868-27871 CD denotes 7.5
T10264 27871-27872 CD denotes ×
T10265 27872-27875 CD denotes 105
T10266 27876-27884 NNP denotes cells/mL
T10267 27884-27885 -RRB- denotes )
T10268 27885-27886 . denotes .
T10269 27887-27892 CD denotes THP-1
T10270 27893-27901 NN denotes reporter
T10271 27902-27907 NNS denotes cells
T10272 27908-27912 VBD denotes were
T10273 27913-27927 VBN denotes differentiated
T10274 27928-27932 IN denotes into
T10275 27933-27934 DT denotes a
T10276 27935-27950 JJ denotes macrophage-like
T10277 27951-27960 NN denotes phenotype
T10278 27961-27966 VBG denotes using
T10279 27967-27969 CD denotes 50
T10280 27970-27975 NN denotes ng/ml
T10281 27976-27978 IN denotes of
T10282 27979-27986 NN denotes phorbol
T10283 27987-27999 JJ denotes 12-myristate
T10284 28000-28010 JJ denotes 13-acetate
T10285 28011-28012 -LRB- denotes (
T10286 28012-28015 NNP denotes PMA
T10287 28015-28016 -RRB- denotes )
T10288 28017-28018 -LRB- denotes (
T10289 28018-28023 NNP denotes Sigma
T10290 28024-28031 NNP denotes Aldrich
T10291 28031-28032 -RRB- denotes )
T10292 28033-28036 IN denotes for
T10293 28037-28039 CD denotes 24
T10294 28040-28045 NNS denotes hours
T10295 28046-28048 IN denotes at
T10296 28049-28050 DT denotes a
T10297 28051-28058 NN denotes density
T10298 28059-28061 IN denotes of
T10299 28062-28063 CD denotes 1
T10300 28063-28064 CD denotes ×
T10301 28064-28067 CD denotes 105
T10302 28068-28078 NN denotes cells/well
T10303 28079-28081 IN denotes in
T10304 28082-28085 CD denotes 200
T10305 28086-28088 NN denotes µl
T10306 28089-28090 -LRB- denotes (
T10307 28090-28091 CD denotes 5
T10308 28091-28092 CD denotes ×
T10309 28092-28095 CD denotes 105
T10310 28096-28104 NN denotes cells/ml
T10311 28104-28105 -RRB- denotes )
T10312 28105-28106 . denotes .
T10313 28107-28112 NNS denotes Cells
T10314 28113-28117 VBD denotes were
T10315 28118-28124 VBN denotes washed
T10316 28125-28129 IN denotes with
T10317 28130-28133 NNP denotes PBS
T10318 28134-28137 CC denotes and
T10319 28138-28145 VBN denotes allowed
T10320 28146-28148 TO denotes to
T10321 28149-28153 VB denotes rest
T10322 28154-28155 DT denotes a
T10323 28156-28163 JJ denotes further
T10324 28164-28166 CD denotes 42
T10325 28167-28172 NNS denotes hours
T10326 28173-28178 RB denotes prior
T10327 28179-28181 TO denotes to
T10328 28182-28193 NN denotes stimulation
T10329 28193-28194 . denotes .
T10630 28196-28198 NNP denotes CF
T10631 28199-28202 CC denotes and
T10632 28203-28210 VB denotes control
T10633 28211-28218 JJ denotes subject
T10634 28219-28224 NNP denotes PBMCs
T10635 28225-28228 DT denotes The
T10636 28229-28238 NN denotes diagnosis
T10637 28239-28241 IN denotes of
T10638 28242-28244 NNP denotes CF
T10639 28245-28248 VBD denotes was
T10640 28249-28260 VBN denotes established
T10641 28261-28263 IN denotes by
T10642 28264-28271 JJ denotes typical
T10643 28272-28280 JJ denotes clinical
T10644 28281-28289 NNS denotes features
T10645 28289-28290 , denotes ,
T10646 28291-28300 VBD denotes increased
T10647 28301-28306 NN denotes sweat
T10648 28307-28315 NN denotes chloride
T10649 28316-28330 NNS denotes concentrations
T10650 28331-28332 -LRB- denotes (
T10651 28332-28333 CD denotes >
T10652 28333-28335 CD denotes 60
T10653 28336-28342 NN denotes mmol/l
T10654 28342-28343 -RRB- denotes )
T10655 28343-28344 , denotes ,
T10656 28345-28348 CC denotes and
T10657 28349-28358 NN denotes detection
T10658 28359-28361 IN denotes of
T10659 28362-28373 VBG denotes CF-inducing
T10660 28374-28383 NNS denotes mutations
T10661 28383-28384 . denotes .
T10662 28385-28388 DT denotes All
T10663 28389-28397 NNS denotes patients
T10664 28398-28402 IN denotes with
T10665 28403-28405 NNP denotes CF
T10666 28406-28410 VBD denotes were
T10667 28411-28421 RB denotes clinically
T10668 28422-28428 JJ denotes stable
T10669 28429-28431 IN denotes at
T10670 28432-28435 DT denotes the
T10671 28436-28440 NN denotes time
T10672 28441-28443 IN denotes of
T10673 28444-28449 NN denotes blood
T10674 28450-28458 NN denotes donation
T10675 28458-28459 , denotes ,
T10676 28460-28463 CC denotes and
T10677 28464-28466 PRP denotes we
T10678 28467-28475 VBD denotes excluded
T10679 28476-28479 DT denotes any
T10680 28480-28488 NNS denotes subjects
T10681 28489-28492 WP denotes who
T10682 28493-28497 VBD denotes were
T10683 28498-28507 VBG denotes receiving
T10684 28508-28516 JJ denotes systemic
T10685 28517-28532 NNS denotes corticosteroids
T10686 28533-28536 JJ denotes due
T10687 28537-28539 TO denotes to
T10688 28540-28549 JJ denotes potential
T10689 28550-28566 JJ denotes immunomodulatory
T10690 28567-28575 NN denotes activity
T10691 28575-28576 . denotes .
T10692 28577-28584 NNP denotes Control
T10693 28585-28592 NNS denotes samples
T10694 28593-28597 VBD denotes were
T10695 28598-28606 VBN denotes provided
T10696 28607-28609 IN denotes by
T10697 28610-28617 JJ denotes healthy
T10698 28618-28623 NN denotes adult
T10699 28624-28634 NNS denotes volunteers
T10700 28634-28635 . denotes .
T10701 28636-28638 IN denotes In
T10702 28639-28649 RB denotes previously
T10703 28650-28659 VBN denotes published
T10704 28660-28664 NN denotes work
T10705 28665-28667 PRP denotes we
T10706 28668-28672 VBP denotes have
T10707 28673-28685 VBN denotes demonstrated
T10708 28686-28690 IN denotes that
T10709 28691-28703 JJ denotes TLR-mediated
T10710 28704-28716 JJ denotes inflammatory
T10711 28717-28726 NNS denotes responses
T10712 28727-28730 VBP denotes are
T10713 28731-28737 JJ denotes stable
T10714 28738-28740 IN denotes in
T10715 28741-28747 NNS denotes humans
T10716 28748-28752 IN denotes from
T10717 28753-28758 NN denotes birth
T10718 28759-28761 TO denotes to
T10719 28762-28764 CD denotes 60
T10720 28765-28770 NNS denotes years
T10721 28771-28774 JJ denotes old
T10722 28775-28776 NNP denotes [
T10723 28776-28778 CD denotes 63
T10724 28778-28779 NNP denotes ]
T10725 28779-28780 , denotes ,
T10726 28781-28790 RB denotes therefore
T10727 28791-28794 VBD denotes did
T10728 28795-28798 RB denotes not
T10729 28799-28808 JJ denotes age-match
T10730 28809-28812 DT denotes the
T10731 28813-28815 NNP denotes CF
T10732 28816-28824 NNS denotes patients
T10733 28825-28828 CC denotes and
T10734 28829-28836 NN denotes control
T10735 28837-28845 NNS denotes subjects
T10736 28845-28846 . denotes .
T10737 28847-28857 JJ denotes Peripheral
T10738 28858-28863 NN denotes blood
T10739 28864-28867 VBD denotes was
T10740 28868-28877 VBN denotes collected
T10741 28878-28880 IN denotes in
T10742 28881-28887 NN denotes sodium
T10743 28888-28895 NN denotes heparin
T10744 28896-28901 NNS denotes tubes
T10745 28902-28903 -LRB- denotes (
T10746 28903-28905 NNP denotes BD
T10747 28906-28917 NNPS denotes Biosciences
T10748 28917-28918 -RRB- denotes )
T10749 28919-28922 CC denotes and
T10750 28923-28928 NNS denotes PBMCs
T10751 28929-28933 VBD denotes were
T10752 28934-28942 VBN denotes isolated
T10753 28943-28948 VBG denotes using
T10754 28949-28956 NN denotes density
T10755 28957-28965 NN denotes gradient
T10756 28966-28980 NN denotes centrifugation
T10757 28981-28983 IN denotes on
T10758 28984-28996 NNP denotes Ficoll-Paque
T10759 28996-28997 NNP denotes ™
T10760 28998-29002 NNP denotes Plus
T10761 29003-29004 -LRB- denotes (
T10762 29004-29006 NNP denotes GE
T10763 29007-29017 NNP denotes Healthcare
T10764 29017-29018 -RRB- denotes )
T10765 29018-29019 . denotes .
T10766 29020-29023 DT denotes The
T10767 29024-29029 NN denotes layer
T10768 29030-29040 VBG denotes containing
T10769 29041-29046 NNP denotes PBMCs
T10770 29047-29050 VBD denotes was
T10771 29051-29059 VBN denotes isolated
T10772 29059-29060 , denotes ,
T10773 29061-29067 VBN denotes washed
T10774 29068-29073 RB denotes twice
T10775 29074-29076 IN denotes in
T10776 29077-29080 NNP denotes PBS
T10777 29081-29084 CC denotes and
T10778 29085-29096 VBD denotes resuspended
T10779 29097-29099 IN denotes in
T10780 29100-29108 JJ denotes complete
T10781 29109-29113 NNP denotes RPMI
T10782 29113-29114 . denotes .
T10783 29115-29120 NNS denotes Cells
T10784 29121-29125 VBD denotes were
T10785 29126-29136 VBN denotes enumerated
T10786 29137-29139 IN denotes by
T10787 29140-29146 NN denotes trypan
T10788 29147-29151 JJ denotes blue
T10789 29152-29161 NN denotes exclusion
T10790 29162-29167 VBG denotes using
T10791 29168-29171 DT denotes the
T10792 29172-29180 NNP denotes Countess
T10793 29181-29190 VBN denotes automated
T10794 29191-29195 NN denotes cell
T10795 29196-29203 NN denotes counter
T10796 29204-29205 -LRB- denotes (
T10797 29205-29215 NNP denotes Invitrogen
T10798 29215-29216 -RRB- denotes )
T10799 29216-29217 . denotes .
T10800 29218-29221 IN denotes For
T10801 29222-29232 NN denotes derivation
T10802 29233-29235 IN denotes of
T10803 29236-29247 NNS denotes macrophages
T10804 29248-29252 IN denotes from
T10805 29253-29262 NNS denotes monocytes
T10806 29262-29263 , denotes ,
T10807 29264-29273 NNS denotes monocytes
T10808 29274-29278 VBD denotes were
T10809 29279-29286 VBN denotes allowed
T10810 29287-29289 TO denotes to
T10811 29290-29296 VB denotes adhere
T10812 29297-29299 TO denotes to
T10813 29300-29307 NN denotes plastic
T10814 29308-29311 IN denotes for
T10815 29312-29313 CD denotes 2
T10816 29314-29319 NNS denotes hours
T10817 29320-29322 IN denotes in
T10818 29323-29327 NNP denotes RPMI
T10819 29328-29332 CD denotes 1640
T10820 29333-29338 IN denotes after
T10821 29339-29344 WDT denotes which
T10822 29345-29357 JJ denotes non-adherent
T10823 29358-29363 NNS denotes cells
T10824 29364-29368 VBD denotes were
T10825 29369-29376 VBN denotes removed
T10826 29376-29377 . denotes .
T10827 29378-29387 NNS denotes Monocytes
T10828 29388-29392 VBD denotes were
T10829 29393-29400 VBN denotes allowed
T10830 29401-29403 TO denotes to
T10831 29404-29417 VB denotes differentiate
T10832 29418-29420 IN denotes in
T10833 29421-29425 NNP denotes RPMI
T10834 29426-29430 CD denotes 1640
T10835 29431-29443 VBN denotes supplemented
T10836 29444-29448 IN denotes with
T10837 29449-29451 CD denotes 10
T10838 29451-29452 NN denotes %
T10839 29453-29458 JJ denotes human
T10840 29459-29461 NNP denotes AB
T10841 29462-29467 NN denotes serum
T10842 29468-29471 IN denotes for
T10843 29472-29474 CD denotes 10
T10844 29475-29479 NNS denotes days
T10845 29479-29480 . denotes .
T11104 29482-29486 NNP denotes Cell
T11105 29487-29498 NN denotes stimulation
T11106 29499-29502 CC denotes and
T11107 29503-29511 NN denotes cytokine
T11108 29512-29526 NN denotes quantification
T11109 29527-29536 NNP denotes Bronchial
T11110 29537-29547 JJ denotes epithelial
T11111 29548-29553 NNS denotes cells
T11112 29554-29558 VBD denotes were
T11113 29559-29565 VBN denotes plated
T11114 29566-29569 CC denotes and
T11115 29570-29577 VBN denotes allowed
T11116 29578-29580 TO denotes to
T11117 29581-29587 VB denotes adhere
T11118 29588-29597 JJ denotes overnight
T11119 29598-29603 RB denotes prior
T11120 29604-29606 TO denotes to
T11121 29607-29618 NN denotes stimulation
T11122 29618-29619 . denotes .
T11123 29620-29629 NNP denotes Bronchial
T11124 29630-29640 JJ denotes epithelial
T11125 29641-29646 NNS denotes cells
T11126 29647-29651 VBD denotes were
T11127 29652-29658 VBN denotes rested
T11128 29659-29661 CC denotes or
T11129 29662-29668 VBN denotes primed
T11130 29669-29673 IN denotes with
T11131 29674-29677 NNP denotes LPS
T11132 29678-29681 IN denotes for
T11133 29682-29683 CD denotes 5
T11134 29684-29689 NNS denotes hours
T11135 29690-29693 CC denotes and
T11136 29694-29704 VBN denotes stimulated
T11137 29705-29709 IN denotes with
T11138 29710-29714 JJ denotes live
T11139 29715-29717 NNP denotes P.
T11140 29718-29728 NN denotes aeruginosa
T11141 29729-29733 NNP denotes PAO1
T11142 29734-29736 CC denotes or
T11143 29737-29740 NNP denotes ATP
T11144 29741-29744 IN denotes for
T11145 29745-29748 DT denotes the
T11146 29749-29754 NNS denotes times
T11147 29755-29764 VBD denotes indicated
T11148 29764-29765 . denotes .
T11149 29766-29771 NNS denotes PBMCs
T11150 29772-29775 CC denotes and
T11151 29776-29781 CD denotes THP-1
T11152 29782-29790 NN denotes reporter
T11153 29791-29796 NNS denotes cells
T11154 29797-29801 VBD denotes were
T11155 29802-29808 RB denotes either
T11156 29809-29815 VBN denotes rested
T11157 29816-29818 CC denotes or
T11158 29819-29825 VBN denotes primed
T11159 29826-29830 IN denotes with
T11160 29831-29834 NNP denotes LPS
T11161 29835-29836 -LRB- denotes (
T11162 29836-29845 NNP denotes Invivogen
T11163 29845-29846 -RRB- denotes )
T11164 29847-29849 CC denotes or
T11165 29850-29861 JJ denotes heat-killed
T11166 29862-29866 NNP denotes PAO1
T11167 29867-29876 JJ denotes overnight
T11168 29877-29878 -LRB- denotes (
T11169 29878-29880 CD denotes 16
T11170 29881-29886 NNS denotes hours
T11171 29886-29887 -RRB- denotes )
T11172 29887-29888 . denotes .
T11173 29889-29892 DT denotes The
T11174 29893-29897 JJ denotes next
T11175 29898-29901 NN denotes day
T11176 29902-29905 DT denotes the
T11177 29906-29911 NNS denotes cells
T11178 29912-29916 VBD denotes were
T11179 29917-29927 VBN denotes challenged
T11180 29928-29932 IN denotes with
T11181 29933-29937 JJ denotes live
T11182 29938-29942 NNP denotes PAO1
T11183 29942-29943 , denotes ,
T11184 29944-29953 NNP denotes PAO1ΔexsA
T11185 29953-29954 , denotes ,
T11186 29955-29958 NNP denotes ATP
T11187 29959-29960 -LRB- denotes (
T11188 29960-29969 NNP denotes Invivogen
T11189 29969-29970 -RRB- denotes )
T11190 29970-29971 , denotes ,
T11191 29972-29974 CC denotes or
T11192 29975-29979 NNP denotes Poly
T11193 29979-29980 -LRB- denotes (
T11194 29980-29982 NNP denotes dA
T11195 29982-29983 : denotes :
T11196 29983-29985 NN denotes dT
T11197 29985-29986 -RRB- denotes )
T11198 29987-29988 -LRB- denotes (
T11199 29988-29993 NNP denotes Sigma
T11200 29994-30001 NNP denotes Aldrich
T11201 30001-30002 -RRB- denotes )
T11202 30003-30006 IN denotes for
T11203 30007-30010 DT denotes the
T11204 30011-30016 NNS denotes times
T11205 30017-30026 VBD denotes indicated
T11206 30027-30028 -LRB- denotes (
T11207 30028-30031 VB denotes see
T11208 30032-30036 NNP denotes Fig.
T11209 30037-30038 CD denotes 1
T11210 30038-30039 -RRB- denotes )
T11211 30039-30040 . denotes .
T11212 30041-30044 IN denotes For
T11213 30045-30057 NNS denotes stimulations
T11214 30058-30062 IN denotes with
T11215 30063-30067 NNP denotes Poly
T11216 30067-30068 -LRB- denotes (
T11217 30068-30070 NNP denotes dA
T11218 30070-30071 : denotes :
T11219 30071-30073 NN denotes dT
T11220 30073-30074 -RRB- denotes )
T11221 30074-30075 , denotes ,
T11222 30076-30089 NN denotes lipofectamine
T11223 30090-30093 NNP denotes LTX
T11224 30094-30097 VBD denotes was
T11225 30098-30102 VBN denotes used
T11226 30103-30105 IN denotes at
T11227 30106-30107 DT denotes a
T11228 30108-30109 CD denotes 1
T11229 30109-30110 NN denotes ∶
T11230 30110-30111 CD denotes 1
T11231 30112-30113 -LRB- denotes (
T11232 30113-30114 FW denotes w
T11233 30114-30115 FW denotes ∶
T11234 30115-30116 FW denotes v
T11235 30116-30117 -RRB- denotes )
T11236 30118-30123 NN denotes ratio
T11237 30124-30126 IN denotes of
T11238 30127-30129 NN denotes µg
T11239 30130-30132 IN denotes of
T11240 30133-30136 NNP denotes DNA
T11241 30137-30139 TO denotes to
T11242 30140-30142 VB denotes µl
T11243 30143-30145 IN denotes of
T11244 30146-30159 NN denotes lipofectamine
T11245 30160-30163 CC denotes and
T11246 30164-30167 VBD denotes was
T11247 30168-30173 JJ denotes mixed
T11248 30174-30176 CD denotes 30
T11249 30177-30184 NNS denotes minutes
T11250 30185-30190 RB denotes prior
T11251 30191-30193 TO denotes to
T11252 30194-30205 NN denotes stimulation
T11253 30205-30206 . denotes .
T11254 30207-30210 DT denotes The
T11255 30211-30216 JJ denotes NF-κB
T11256 30217-30226 NN denotes inhibitor
T11257 30227-30237 NN denotes Bay11-7082
T11258 30238-30239 -LRB- denotes (
T11259 30239-30248 NNP denotes Invivogen
T11260 30248-30249 -RRB- denotes )
T11261 30250-30253 VBD denotes was
T11262 30254-30259 VBN denotes added
T11263 30260-30262 TO denotes to
T11264 30263-30271 NNS denotes cultures
T11265 30272-30273 CD denotes 1
T11266 30274-30278 NN denotes hour
T11267 30279-30284 RB denotes prior
T11268 30285-30287 TO denotes to
T11269 30288-30295 VBG denotes priming
T11270 30295-30296 . denotes .
T11271 30297-30299 IN denotes If
T11272 30300-30302 DT denotes no
T11273 30303-30310 NN denotes priming
T11274 30311-30314 VBD denotes was
T11275 30315-30323 VBN denotes involved
T11276 30323-30324 , denotes ,
T11277 30325-30334 NN denotes inhibitor
T11278 30335-30338 VBD denotes was
T11279 30339-30344 VBN denotes added
T11280 30345-30346 CD denotes 1
T11281 30347-30351 NN denotes hour
T11282 30352-30357 RB denotes prior
T11283 30358-30360 TO denotes to
T11284 30361-30373 JJ denotes inflammasome
T11285 30374-30385 NN denotes stimulation
T11286 30385-30386 . denotes .
T11287 30387-30390 DT denotes The
T11288 30391-30395 NNP denotes CFTR
T11289 30396-30405 NN denotes inhibitor
T11290 30406-30416 NNP denotes CFTRinh172
T11291 30417-30418 -LRB- denotes (
T11292 30418-30423 NNP denotes Sigma
T11293 30424-30431 NNP denotes Aldrich
T11294 30431-30432 -RRB- denotes )
T11295 30433-30436 VBD denotes was
T11296 30437-30442 VBN denotes added
T11297 30443-30445 TO denotes to
T11298 30446-30454 NNS denotes cultures
T11299 30455-30457 CD denotes 18
T11300 30458-30463 NNS denotes hours
T11301 30464-30469 RB denotes prior
T11302 30470-30472 TO denotes to
T11303 30473-30485 JJ denotes inflammasome
T11304 30486-30497 NN denotes stimulation
T11305 30497-30498 . denotes .
T11306 30499-30502 DT denotes The
T11307 30503-30512 JJ denotes caspase-1
T11308 30513-30522 NN denotes inhibitor
T11309 30523-30533 NNP denotes z-YVAD-fmk
T11310 30534-30535 -LRB- denotes (
T11311 30535-30544 NNP denotes Biovision
T11312 30544-30545 -RRB- denotes )
T11313 30546-30549 VBD denotes was
T11314 30550-30555 VBN denotes added
T11315 30556-30558 TO denotes to
T11316 30559-30567 NNS denotes cultures
T11317 30568-30569 CD denotes 1
T11318 30570-30574 NN denotes hour
T11319 30575-30580 RB denotes prior
T11320 30581-30583 TO denotes to
T11321 30584-30596 JJ denotes inflammasome
T11322 30597-30608 NN denotes stimulation
T11323 30608-30609 . denotes .
T11324 30610-30622 NNS denotes Supernatants
T11325 30623-30627 VBD denotes were
T11326 30628-30637 VBN denotes collected
T11327 30638-30641 CC denotes and
T11328 30642-30648 VBN denotes stored
T11329 30649-30651 IN denotes at
T11330 30652-30653 CD denotes −
T11331 30653-30655 CD denotes 20
T11332 30655-30656 NN denotes °
T11333 30656-30658 NNP denotes C.
T11334 30659-30668 NNP denotes Cytokines
T11335 30669-30677 VBD denotes released
T11336 30678-30682 IN denotes into
T11337 30683-30695 NNS denotes supernatants
T11338 30696-30700 IN denotes from
T11339 30701-30706 NNP denotes PBMCs
T11340 30707-30717 VBD denotes stimulated
T11341 30718-30722 IN denotes with
T11342 30723-30735 JJ denotes inflammasome
T11343 30736-30746 NNS denotes activators
T11344 30747-30751 VBD denotes were
T11345 30752-30762 VBN denotes quantified
T11346 30763-30768 VBG denotes using
T11347 30769-30777 NN denotes sandwich
T11348 30778-30783 NNP denotes ELISA
T11349 30784-30785 -LRB- denotes (
T11350 30785-30796 NNP denotes eBioscience
T11351 30796-30797 -RRB- denotes )
T11352 30797-30798 . denotes .
T11553 30800-30814 VBG denotes Immunoblotting
T11554 30815-30816 CD denotes 1
T11555 30816-30817 CD denotes ×
T11556 30817-30820 CD denotes 106
T11557 30821-30826 NNS denotes cells
T11558 30827-30831 VBD denotes were
T11559 30832-30838 VBN denotes seeded
T11560 30839-30841 IN denotes in
T11561 30842-30849 JJ denotes 12-well
T11562 30850-30856 NNS denotes plates
T11563 30856-30857 , denotes ,
T11564 30858-30868 VBN denotes stimulated
T11565 30869-30871 IN denotes as
T11566 30872-30881 VBN denotes indicated
T11567 30881-30882 , denotes ,
T11568 30883-30886 CC denotes and
T11569 30887-30892 VBD denotes lysed
T11570 30893-30895 IN denotes in
T11571 30896-30900 NNP denotes RIPA
T11572 30901-30907 NN denotes buffer
T11573 30908-30920 VBN denotes supplemented
T11574 30921-30925 IN denotes with
T11575 30926-30930 NNP denotes Halt
T11576 30931-30939 NN denotes protease
T11577 30940-30943 CC denotes and
T11578 30944-30955 NN denotes phosphatase
T11579 30956-30965 NN denotes inhibitor
T11580 30966-30974 NN denotes cocktail
T11581 30975-30976 -LRB- denotes (
T11582 30976-30982 NNP denotes Thermo
T11583 30983-30993 NNP denotes Scientific
T11584 30993-30994 -RRB- denotes )
T11585 30994-30995 . denotes .
T11586 30996-31003 NN denotes Protein
T11587 31004-31018 NNS denotes concentrations
T11588 31019-31023 VBD denotes were
T11589 31024-31034 VBN denotes determined
T11590 31035-31037 IN denotes by
T11591 31038-31046 NNP denotes Bradford
T11592 31047-31052 NN denotes assay
T11593 31053-31054 -LRB- denotes (
T11594 31054-31060 NNP denotes Thermo
T11595 31061-31071 NNP denotes Scientific
T11596 31071-31072 -RRB- denotes )
T11597 31072-31073 . denotes .
T11598 31074-31081 NNS denotes Lysates
T11599 31082-31086 VBD denotes were
T11600 31087-31095 VBN denotes resolved
T11601 31096-31098 IN denotes by
T11602 31099-31114 NNS denotes electrophoreses
T11603 31115-31117 IN denotes on
T11604 31118-31120 CD denotes 10
T11605 31120-31121 NN denotes %
T11606 31122-31140 JJ denotes SDS-polyacrylamide
T11607 31141-31145 NNS denotes gels
T11608 31146-31149 CC denotes and
T11609 31150-31161 VBN denotes transferred
T11610 31162-31166 IN denotes onto
T11611 31167-31171 NNP denotes PVDF
T11612 31172-31181 NNS denotes membranes
T11613 31182-31183 -LRB- denotes (
T11614 31183-31192 NNP denotes Millipore
T11615 31192-31193 -RRB- denotes )
T11616 31193-31194 . denotes .
T11617 31195-31200 NNS denotes Blots
T11618 31201-31205 VBD denotes were
T11619 31206-31213 VBN denotes blocked
T11620 31214-31217 IN denotes for
T11621 31218-31219 CD denotes 1
T11622 31220-31224 NN denotes hour
T11623 31225-31227 IN denotes at
T11624 31228-31232 NN denotes room
T11625 31233-31244 NN denotes temperature
T11626 31245-31248 CC denotes and
T11627 31249-31255 VBD denotes probed
T11628 31256-31265 JJ denotes overnight
T11629 31266-31268 IN denotes at
T11630 31269-31270 CD denotes 4
T11631 31270-31271 CD denotes °
T11632 31271-31272 NNP denotes C
T11633 31273-31276 IN denotes for
T11634 31277-31286 NNP denotes pro-IL-1β
T11635 31287-31288 -LRB- denotes (
T11636 31288-31293 NNP denotes Santa
T11637 31294-31298 NNP denotes Cruz
T11638 31298-31299 -RRB- denotes )
T11639 31299-31300 , denotes ,
T11640 31301-31305 NNP denotes IκBα
T11641 31306-31307 -LRB- denotes (
T11642 31307-31311 NNP denotes Cell
T11643 31312-31321 NNP denotes Signaling
T11644 31321-31322 -RRB- denotes )
T11645 31322-31323 , denotes ,
T11646 31324-31326 CC denotes or
T11647 31327-31334 NN denotes β-actin
T11648 31335-31336 -LRB- denotes (
T11649 31336-31340 NNP denotes Cell
T11650 31341-31350 NNP denotes Signaling
T11651 31350-31351 -RRB- denotes )
T11652 31351-31352 . denotes .
T11653 31353-31358 NNS denotes Blots
T11654 31359-31363 VBD denotes were
T11655 31364-31376 RB denotes subsequently
T11656 31377-31383 VBN denotes probed
T11657 31384-31388 IN denotes with
T11658 31389-31410 JJ denotes fluorescently-labeled
T11659 31411-31420 JJ denotes secondary
T11660 31421-31431 NNS denotes antibodies
T11661 31431-31432 , denotes ,
T11662 31433-31438 NNP denotes IRDye
T11663 31438-31439 NNP denotes ®
T11664 31440-31443 CD denotes 680
T11665 31444-31446 CC denotes or
T11666 31447-31452 CD denotes 800CW
T11667 31453-31454 -LRB- denotes (
T11668 31454-31460 NNP denotes LI-COR
T11669 31461-31472 NNPS denotes Biosciences
T11670 31472-31473 -RRB- denotes )
T11671 31474-31477 IN denotes for
T11672 31478-31479 CD denotes 1
T11673 31480-31484 NN denotes hour
T11674 31484-31485 . denotes .
T11675 31486-31490 DT denotes Both
T11676 31491-31499 VBG denotes blocking
T11677 31500-31503 CC denotes and
T11678 31504-31511 VBG denotes probing
T11679 31512-31517 NNS denotes steps
T11680 31518-31522 VBD denotes were
T11681 31523-31530 VBN denotes carried
T11682 31531-31534 RP denotes out
T11683 31535-31537 IN denotes in
T11684 31538-31551 JJ denotes tris-buffered
T11685 31552-31558 NN denotes saline
T11686 31559-31560 -LRB- denotes (
T11687 31560-31561 NNP denotes G
T11688 31562-31573 NNPS denotes Biosciences
T11689 31573-31574 -RRB- denotes )
T11690 31575-31585 VBG denotes containing
T11691 31586-31587 CD denotes 5
T11692 31587-31588 NN denotes %
T11693 31589-31595 JJ denotes bovine
T11694 31596-31601 NN denotes serum
T11695 31602-31609 NN denotes albumin
T11696 31610-31613 CC denotes and
T11697 31614-31617 CD denotes 0.1
T11698 31617-31618 NN denotes %
T11699 31619-31624 NNP denotes TWEEN
T11700 31625-31627 CD denotes 20
T11701 31628-31629 -LRB- denotes (
T11702 31629-31639 NN denotes Calbiochem
T11703 31639-31640 -RRB- denotes )
T11704 31640-31641 . denotes .
T11705 31642-31647 NNS denotes Blots
T11706 31648-31652 VBD denotes were
T11707 31653-31659 VBN denotes imaged
T11708 31660-31662 IN denotes on
T11709 31663-31664 DT denotes a
T11710 31665-31671 NNP denotes LI-COR
T11711 31672-31679 NNP denotes Odyssey
T11712 31680-31688 VBD denotes infrared
T11713 31689-31696 NN denotes imaging
T11714 31697-31703 NN denotes system
T11715 31704-31705 -LRB- denotes (
T11716 31705-31711 NNP denotes LI-COR
T11717 31712-31723 NNPS denotes Biosciences
T11718 31723-31724 -RRB- denotes )
T11719 31725-31728 CC denotes and
T11720 31729-31739 VBD denotes quantified
T11721 31740-31745 VBG denotes using
T11722 31746-31749 DT denotes the
T11723 31750-31758 VBN denotes included
T11724 31759-31767 NN denotes analysis
T11725 31768-31776 NN denotes software
T11726 31776-31777 . denotes .
T11929 31779-31793 NN denotes Quantification
T11930 31794-31796 IN denotes of
T11931 31797-31806 JJ denotes caspase-1
T11932 31807-31815 NN denotes activity
T11933 31816-31825 NNP denotes Bronchial
T11934 31826-31836 JJ denotes epithelial
T11935 31837-31842 NNS denotes cells
T11936 31843-31847 VBD denotes were
T11937 31848-31854 VBN denotes plated
T11938 31855-31857 IN denotes in
T11939 31858-31864 JJ denotes 6-well
T11940 31865-31871 NNS denotes plates
T11941 31872-31874 IN denotes at
T11942 31875-31876 CD denotes 5
T11943 31876-31877 CD denotes ×
T11944 31877-31880 CD denotes 105
T11945 31881-31891 NN denotes cells/well
T11946 31892-31901 JJ denotes overnight
T11947 31901-31902 . denotes .
T11948 31903-31908 NNS denotes Cells
T11949 31909-31913 VBD denotes were
T11950 31914-31920 VBN denotes primed
T11951 31921-31925 IN denotes with
T11952 31926-31929 NNP denotes LPS
T11953 31930-31933 IN denotes for
T11954 31934-31935 CD denotes 5
T11955 31936-31941 NNS denotes hours
T11956 31942-31945 CC denotes and
T11957 31946-31956 VBN denotes stimulated
T11958 31957-31961 IN denotes with
T11959 31962-31965 NNP denotes ATP
T11960 31966-31969 IN denotes for
T11961 31970-31971 CD denotes 1
T11962 31972-31976 NN denotes hour
T11963 31977-31979 CC denotes or
T11964 31980-31990 VBN denotes stimulated
T11965 31991-31995 IN denotes with
T11966 31996-32000 JJ denotes live
T11967 32001-32005 NN denotes PAO1
T11968 32006-32009 IN denotes for
T11969 32010-32011 CD denotes 3
T11970 32012-32017 NNS denotes hours
T11971 32017-32018 . denotes .
T11972 32019-32024 NNS denotes PBMCs
T11973 32025-32029 VBD denotes were
T11974 32030-32040 VBN denotes stimulated
T11975 32041-32044 DT denotes the
T11976 32045-32049 JJ denotes same
T11977 32050-32053 NN denotes day
T11978 32054-32056 IN denotes as
T11979 32057-32062 NN denotes blood
T11980 32063-32071 NN denotes donation
T11981 32071-32072 . denotes .
T11982 32073-32078 NNS denotes PBMCs
T11983 32079-32083 VBD denotes were
T11984 32084-32090 VBN denotes seeded
T11985 32091-32093 IN denotes in
T11986 32094-32095 DT denotes a
T11987 32096-32103 JJ denotes 96-well
T11988 32104-32109 NN denotes plate
T11989 32110-32112 IN denotes at
T11990 32113-32114 DT denotes a
T11991 32115-32122 NN denotes density
T11992 32123-32125 IN denotes of
T11993 32126-32129 CD denotes 4.5
T11994 32129-32130 CD denotes ×
T11995 32130-32133 CD denotes 105
T11996 32134-32144 NN denotes cells/well
T11997 32145-32146 -LRB- denotes (
T11998 32146-32149 CD denotes 2.5
T11999 32149-32150 CD denotes ×
T12000 32150-32153 CD denotes 106
T12001 32154-32162 NN denotes cells/ml
T12002 32162-32163 -RRB- denotes )
T12003 32163-32164 , denotes ,
T12004 32165-32171 VBN denotes primed
T12005 32172-32176 IN denotes with
T12006 32177-32180 NNP denotes LPS
T12007 32181-32184 IN denotes for
T12008 32185-32186 CD denotes 5
T12009 32187-32192 NNS denotes hours
T12010 32193-32196 CC denotes and
T12011 32197-32201 RB denotes then
T12012 32202-32212 VBN denotes stimulated
T12013 32213-32217 IN denotes with
T12014 32218-32221 NNP denotes ATP
T12015 32222-32225 IN denotes for
T12016 32226-32227 CD denotes 1
T12017 32228-32232 NN denotes hour
T12018 32233-32235 CC denotes or
T12019 32236-32240 NNP denotes Poly
T12020 32240-32241 -LRB- denotes (
T12021 32241-32243 NNP denotes dA
T12022 32243-32244 : denotes :
T12023 32244-32246 NN denotes dT
T12024 32246-32247 -RRB- denotes )
T12025 32248-32251 IN denotes for
T12026 32252-32253 CD denotes 3
T12027 32254-32259 NNS denotes hours
T12028 32260-32262 CC denotes or
T12029 32263-32273 VBN denotes stimulated
T12030 32274-32278 IN denotes with
T12031 32279-32283 JJ denotes live
T12032 32284-32288 NN denotes PAO1
T12033 32289-32292 IN denotes for
T12034 32293-32294 CD denotes 3
T12035 32295-32300 NNS denotes hours
T12036 32300-32301 . denotes .
T12037 32302-32311 NN denotes Caspase-1
T12038 32312-32320 NN denotes activity
T12039 32321-32324 VBD denotes was
T12040 32325-32333 VBN denotes measured
T12041 32334-32339 VBG denotes using
T12042 32340-32345 NNP denotes FLICA
T12043 32346-32347 -LRB- denotes (
T12044 32347-32362 NNP denotes Immunochemistry
T12045 32363-32375 NNPS denotes Technologies
T12046 32375-32376 -RRB- denotes )
T12047 32376-32377 , denotes ,
T12048 32378-32379 DT denotes a
T12049 32380-32394 JJ denotes cell-permeable
T12050 32395-32406 JJ denotes fluorescent
T12051 32407-32412 NN denotes probe
T12052 32413-32414 -LRB- denotes (
T12053 32414-32426 JJ denotes FAM-YVAD-fmk
T12054 32426-32427 -RRB- denotes )
T12055 32428-32432 WDT denotes that
T12056 32433-32438 VBZ denotes binds
T12057 32439-32445 JJ denotes active
T12058 32446-32455 NN denotes caspase-1
T12059 32455-32456 . denotes .
T12060 32457-32462 NNS denotes Cells
T12061 32463-32467 VBD denotes were
T12062 32468-32477 VBN denotes incubated
T12063 32478-32479 CD denotes 1
T12064 32480-32484 NN denotes hour
T12065 32485-32489 IN denotes with
T12066 32490-32495 NNP denotes FLICA
T12067 32496-32498 IN denotes at
T12068 32499-32501 CD denotes 37
T12069 32501-32502 CD denotes °
T12070 32502-32503 NNP denotes C
T12071 32504-32507 CC denotes and
T12072 32508-32515 VBN denotes stained
T12073 32516-32520 IN denotes with
T12074 32521-32538 JJ denotes PE-Cy7-conjugated
T12075 32539-32548 JJ denotes anti-CD14
T12076 32549-32559 NNS denotes antibodies
T12077 32560-32561 -LRB- denotes (
T12078 32561-32572 NNP denotes eBioscience
T12079 32572-32573 -RRB- denotes )
T12080 32574-32576 TO denotes to
T12081 32577-32585 VB denotes identify
T12082 32586-32595 NNS denotes monocytes
T12083 32595-32596 . denotes .
T12084 32597-32600 DT denotes The
T12085 32601-32607 VBG denotes gating
T12086 32608-32616 NN denotes strategy
T12087 32617-32626 VBD denotes consisted
T12088 32627-32629 IN denotes of
T12089 32630-32639 VBG denotes including
T12090 32640-32644 JJ denotes live
T12091 32645-32650 NNS denotes cells
T12092 32651-32655 WDT denotes that
T12093 32656-32660 VBD denotes were
T12094 32661-32665 CD denotes CD14
T12095 32666-32674 JJ denotes positive
T12096 32675-32680 WDT denotes which
T12097 32681-32685 VBD denotes were
T12098 32686-32698 RB denotes subsequently
T12099 32699-32707 VBN denotes analyzed
T12100 32708-32711 IN denotes for
T12101 32712-32715 DT denotes the
T12102 32716-32725 NN denotes frequency
T12103 32726-32728 IN denotes of
T12104 32729-32734 NNP denotes FLICA
T12105 32735-32743 JJ denotes positive
T12106 32744-32749 NNS denotes cells
T12107 32749-32750 . denotes .
T12209 32752-32757 NNP denotes NF-κB
T12210 32757-32758 NNP denotes /
T12211 32758-32762 NNP denotes AP-1
T12212 32763-32771 NNP denotes Activity
T12213 32772-32777 NNP denotes Assay
T12214 32778-32790 NNPS denotes Supernatants
T12215 32791-32795 IN denotes from
T12216 32796-32801 CD denotes THP-1
T12217 32802-32810 NN denotes reporter
T12218 32811-32816 NNS denotes cells
T12219 32817-32821 VBD denotes were
T12220 32822-32831 VBN denotes incubated
T12221 32832-32836 IN denotes with
T12222 32837-32848 JJ denotes Quanti-Blue
T12223 32849-32858 NN denotes substrate
T12224 32859-32860 -LRB- denotes (
T12225 32860-32869 NNP denotes Invivogen
T12226 32869-32870 -RRB- denotes )
T12227 32871-32873 IN denotes at
T12228 32874-32876 CD denotes 37
T12229 32876-32877 CD denotes °
T12230 32877-32878 NNP denotes C
T12231 32879-32882 CC denotes and
T12232 32883-32890 VBN denotes allowed
T12233 32891-32893 TO denotes to
T12234 32894-32901 VB denotes develop
T12235 32902-32905 IN denotes for
T12236 32906-32908 CD denotes 16
T12237 32909-32911 CD denotes 18
T12238 32912-32917 NNS denotes hours
T12239 32917-32918 . denotes .
T12240 32919-32930 JJ denotes Quanti-Blue
T12241 32931-32939 VBZ denotes contains
T12242 32940-32941 DT denotes a
T12243 32942-32951 NN denotes substrate
T12244 32952-32955 IN denotes for
T12245 32956-32964 JJ denotes alkaline
T12246 32965-32976 NN denotes phosphatase
T12247 32977-32980 CC denotes and
T12248 32981-32988 NNS denotes changes
T12249 32989-32991 IN denotes in
T12250 32992-32995 DT denotes the
T12251 32996-33002 NN denotes amount
T12252 33003-33005 IN denotes of
T12253 33006-33011 NNP denotes NF-κB
T12254 33011-33012 NNP denotes /
T12255 33012-33016 NNP denotes AP-1
T12256 33017-33025 NN denotes activity
T12257 33026-33030 VBD denotes were
T12258 33031-33041 VBN denotes quantified
T12259 33042-33044 IN denotes by
T12260 33045-33052 JJ denotes optical
T12261 33053-33060 NN denotes density
T12262 33061-33062 -LRB- denotes (
T12263 33062-33063 FW denotes λ
T12264 33064-33065 SYM denotes =
T12265 33066-33069 CD denotes 655
T12266 33069-33070 -RRB- denotes )
T12267 33071-33079 VBN denotes measured
T12268 33080-33085 VBG denotes using
T12269 33086-33087 DT denotes a
T12270 33088-33098 NNP denotes SpectraMax
T12271 33099-33102 CD denotes 384
T12272 33103-33107 NNP denotes Plus
T12273 33108-33113 NN denotes plate
T12274 33114-33120 NN denotes reader
T12275 33121-33124 CC denotes and
T12276 33125-33132 NNP denotes SoftMax
T12277 33133-33136 FW denotes Pro
T12278 33137-33145 NN denotes software
T12279 33146-33147 -LRB- denotes (
T12280 33147-33156 JJ denotes Molecular
T12281 33157-33164 NNPS denotes Devices
T12282 33164-33165 -RRB- denotes )
T12283 33165-33166 . denotes .
T12443 33168-33177 JJ denotes Bacterial
T12444 33178-33185 NNS denotes strains
T12445 33186-33188 NNP denotes P.
T12446 33189-33199 NN denotes aeruginosa
T12447 33200-33210 NN denotes laboratory
T12448 33211-33218 NNS denotes strains
T12449 33219-33223 NNP denotes PAO1
T12450 33224-33227 CC denotes and
T12451 33228-33231 DT denotes the
T12452 33232-33241 NNP denotes PAO1ΔexsA
T12453 33242-33248 JJ denotes mutant
T12454 33249-33253 VBD denotes were
T12455 33254-33262 VBN denotes obtained
T12456 33263-33267 IN denotes from
T12457 33268-33271 NNP denotes Dr.
T12458 33272-33278 NNP denotes Robert
T12459 33279-33286 NNP denotes Hancock
T12460 33286-33287 . denotes .
T12461 33288-33290 NNP denotes P.
T12462 33291-33301 NN denotes aeruginosa
T12463 33302-33309 NNS denotes strains
T12464 33310-33314 NNP denotes PAO1
T12465 33315-33318 CC denotes and
T12466 33319-33328 NNP denotes PAO1ΔexsA
T12467 33329-33333 VBD denotes were
T12468 33334-33339 VBN denotes grown
T12469 33340-33344 IN denotes from
T12470 33345-33354 JJ denotes overnight
T12471 33355-33363 NNS denotes cultures
T12472 33364-33366 IN denotes in
T12473 33367-33372 NNP denotes Luria
T12474 33373-33380 NNP denotes Bertani
T12475 33381-33382 -LRB- denotes (
T12476 33382-33384 NNP denotes LB
T12477 33384-33385 -RRB- denotes )
T12478 33386-33391 NN denotes broth
T12479 33392-33395 CC denotes and
T12480 33396-33398 NNP denotes LB
T12481 33398-33399 NNP denotes +
T12482 33399-33411 NNP denotes streptomycin
T12483 33412-33413 -LRB- denotes (
T12484 33413-33416 CD denotes 150
T12485 33417-33419 NN denotes µg
T12486 33419-33420 NN denotes /
T12487 33420-33422 NN denotes ml
T12488 33422-33423 -RRB- denotes )
T12489 33424-33429 IN denotes until
T12490 33430-33445 JJ denotes mid-logarithmic
T12491 33446-33451 NN denotes phase
T12492 33451-33452 . denotes .
T12493 33453-33458 NNS denotes Cells
T12494 33459-33463 VBD denotes were
T12495 33464-33470 VBN denotes washed
T12496 33471-33475 RB denotes once
T12497 33476-33478 IN denotes in
T12498 33479-33482 NNP denotes PBS
T12499 33483-33486 CC denotes and
T12500 33487-33498 VBD denotes resuspended
T12501 33499-33501 IN denotes in
T12502 33502-33505 NNP denotes PBS
T12503 33506-33508 TO denotes to
T12504 33509-33511 DT denotes an
T12505 33512-33519 JJ denotes optical
T12506 33520-33527 NN denotes density
T12507 33528-33530 IN denotes of
T12508 33531-33534 CD denotes 0.5
T12509 33535-33536 -LRB- denotes (
T12510 33536-33537 NN denotes λ
T12511 33538-33539 SYM denotes =
T12512 33540-33543 CD denotes 600
T12513 33544-33546 NN denotes nm
T12514 33546-33547 -RRB- denotes )
T12515 33547-33548 . denotes .
T12516 33549-33551 TO denotes To
T12517 33552-33559 VB denotes prepare
T12518 33560-33571 JJ denotes heat-killed
T12519 33572-33580 NNS denotes bacteria
T12520 33580-33581 , denotes ,
T12521 33582-33586 VBP denotes live
T12522 33587-33591 CD denotes PAO1
T12523 33592-33595 VBD denotes was
T12524 33596-33607 VBN denotes resuspended
T12525 33608-33610 IN denotes in
T12526 33611-33614 NNP denotes PBS
T12527 33615-33617 TO denotes to
T12528 33618-33620 DT denotes an
T12529 33621-33628 JJ denotes optical
T12530 33629-33636 NN denotes density
T12531 33637-33639 IN denotes of
T12532 33640-33643 CD denotes 0.5
T12533 33644-33647 CC denotes and
T12534 33648-33654 VBN denotes heated
T12535 33655-33657 IN denotes at
T12536 33658-33660 CD denotes 60
T12537 33660-33661 CD denotes °
T12538 33661-33662 NNP denotes C
T12539 33663-33666 IN denotes for
T12540 33667-33668 CD denotes 1
T12541 33669-33673 NN denotes hour
T12542 33673-33674 . denotes .
T12543 33675-33678 IN denotes For
T12544 33679-33691 NNS denotes stimulations
T12545 33691-33692 , denotes ,
T12546 33693-33697 VBP denotes live
T12547 33698-33702 CD denotes PAO1
T12548 33703-33706 VBD denotes was
T12549 33707-33718 VBN denotes resuspended
T12550 33719-33721 TO denotes to
T12551 33722-33724 DT denotes an
T12552 33725-33732 JJ denotes optical
T12553 33733-33740 NN denotes density
T12554 33741-33743 IN denotes of
T12555 33744-33747 CD denotes 0.5
T12556 33748-33750 IN denotes in
T12557 33751-33754 NNP denotes PBS
T12558 33755-33758 CC denotes and
T12559 33759-33766 JJ denotes further
T12560 33767-33774 VBN denotes diluted
T12561 33775-33777 IN denotes in
T12562 33778-33785 NN denotes culture
T12563 33786-33792 NN denotes medium
T12564 33793-33798 RB denotes prior
T12565 33799-33801 TO denotes to
T12566 33802-33813 NN denotes stimulation
T12567 33814-33816 TO denotes to
T12568 33817-33824 VB denotes achieve
T12569 33825-33828 DT denotes the
T12570 33829-33836 VBN denotes desired
T12571 33837-33849 NN denotes multiplicity
T12572 33850-33852 IN denotes of
T12573 33853-33862 NN denotes infection
T12574 33862-33863 . denotes .
T12575 33864-33875 JJ denotes Heat-killed
T12576 33876-33880 NNP denotes PAO1
T12577 33881-33884 VBD denotes was
T12578 33885-33890 VBN denotes added
T12579 33891-33893 IN denotes in
T12580 33894-33895 DT denotes a
T12581 33896-33902 NN denotes volume
T12582 33903-33913 NN denotes equivalent
T12583 33914-33916 TO denotes to
T12584 33917-33921 DT denotes that
T12585 33922-33926 VBN denotes used
T12586 33927-33929 TO denotes to
T12587 33930-33937 VB denotes achieve
T12588 33938-33940 DT denotes an
T12589 33941-33944 NNP denotes MOI
T12590 33945-33947 IN denotes of
T12591 33948-33949 CD denotes 1
T12592 33950-33953 IN denotes for
T12593 33954-33958 JJ denotes live
T12594 33959-33963 NN denotes PAO1
T12595 33963-33964 . denotes .
T12642 33966-33976 NNPS denotes Statistics
T12643 33977-33980 DT denotes All
T12644 33981-33987 NNS denotes graphs
T12645 33988-33995 VBP denotes display
T12646 33996-33999 DT denotes the
T12647 34000-34004 JJ denotes mean
T12648 34005-34006 NN denotes ±
T12649 34007-34010 NNP denotes SEM
T12650 34011-34014 CC denotes and
T12651 34015-34019 VBD denotes were
T12652 34020-34029 VBN denotes generated
T12653 34030-34034 IN denotes with
T12654 34035-34040 NNP denotes Prism
T12655 34041-34042 CD denotes 5
T12656 34043-34044 -LRB- denotes (
T12657 34044-34052 NNP denotes Graphpad
T12658 34052-34053 -RRB- denotes )
T12659 34053-34054 . denotes .
T12660 34055-34066 NNP denotes Statistical
T12661 34067-34079 NN denotes significance
T12662 34080-34083 VBD denotes was
T12663 34084-34094 VBN denotes determined
T12664 34095-34097 IN denotes by
T12665 34098-34108 VBG denotes performing
T12666 34109-34112 CD denotes one
T12667 34113-34115 CC denotes or
T12668 34116-34123 JJ denotes two-way
T12669 34124-34129 NNP denotes ANOVA
T12670 34130-34133 CC denotes and
T12671 34134-34137 DT denotes the
T12672 34138-34148 NNP denotes Bonferroni
T12673 34149-34158 JJS denotes post-test
T12674 34159-34164 WRB denotes where
T12675 34165-34175 JJ denotes applicable
T12676 34175-34176 . denotes .
T12728 6912-6916 NNP denotes Cell
T12729 6917-6928 NN denotes stimulation
T12730 6929-6932 CC denotes and
T12731 6933-6942 NN denotes inhibitor
T12732 6943-6951 NN denotes schedule
T12733 6951-6952 . denotes .
T12734 6953-6961 NN denotes Schedule
T12735 6962-6970 VBZ denotes outlines
T12736 6971-6974 DT denotes the
T12737 6975-6981 NN denotes timing
T12738 6982-6984 IN denotes of
T12739 6985-6994 NN denotes inhibitor
T12740 6995-7003 NN denotes addition
T12741 7004-7007 CC denotes and
T12742 7008-7015 NN denotes priming
T12743 7016-7018 IN denotes in
T12744 7019-7027 NN denotes relation
T12745 7028-7030 TO denotes to
T12746 7031-7043 JJ denotes inflammasome
T12747 7044-7055 NN denotes stimulation
T12748 7056-7057 -LRB- denotes (
T12749 7057-7058 FW denotes t
T12750 7059-7060 SYM denotes =
T12751 7061-7062 CD denotes 0
T12752 7062-7063 -RRB- denotes )
T12753 7064-7067 IN denotes for
T12754 7068-7073 CD denotes THP-1
T12755 7074-7082 NN denotes reporter
T12756 7083-7086 CC denotes and
T12757 7087-7091 NNP denotes PBMC
T12758 7092-7100 NN denotes cytokine
T12759 7101-7115 NN denotes quantification
T12760 7116-7127 NNS denotes experiments
T12761 7127-7128 . denotes .
T12762 7129-7138 NNP denotes Inhibitor
T12763 7139-7149 NNS denotes treatments
T12764 7150-7153 CC denotes and
T12765 7154-7166 NNS denotes stimulations
T12766 7167-7171 VBD denotes were
T12767 7172-7179 VBN denotes carried
T12768 7180-7183 RP denotes out
T12769 7184-7186 IN denotes as
T12770 7187-7196 VBN denotes described
T12771 7197-7199 IN denotes in
T12772 7200-7203 DT denotes the
T12773 7204-7213 NNPS denotes Materials
T12774 7214-7217 CC denotes and
T12775 7218-7225 NNPS denotes Methods
T12776 7226-7233 NN denotes section
T12777 7233-7234 . denotes .
T12939 7279-7285 NNP denotes Airway
T12940 7286-7296 JJ denotes epithelial
T12941 7297-7302 NNS denotes cells
T12942 7303-7305 VBP denotes do
T12943 7306-7309 RB denotes not
T12944 7310-7323 RB denotes significantly
T12945 7324-7334 VB denotes contribute
T12946 7335-7337 TO denotes to
T12947 7338-7343 JJ denotes IL-1β
T12948 7344-7354 NN denotes production
T12949 7355-7357 IN denotes in
T12950 7358-7366 NN denotes response
T12951 7367-7369 TO denotes to
T12952 7370-7382 JJ denotes inflammasome
T12953 7383-7390 NNS denotes stimuli
T12954 7390-7391 . denotes .
T12955 7392-7399 NNP denotes Control
T12956 7400-7404 NN denotes cell
T12957 7405-7410 NNS denotes lines
T12958 7411-7412 -LRB- denotes (
T12959 7412-7413 -LRB- denotes (
T12960 7413-7414 NNP denotes A
T12961 7414-7415 -RRB- denotes )
T12962 7416-7418 CD denotes S9
T12963 7418-7419 , denotes ,
T12964 7420-7421 -LRB- denotes (
T12965 7421-7422 NNP denotes C
T12966 7422-7423 -RRB- denotes )
T12967 7424-7430 NN denotes NuLi-1
T12968 7430-7431 -RRB- denotes )
T12969 7432-7435 CC denotes and
T12970 7436-7441 PRP$ denotes their
T12971 7442-7455 JJ denotes corresponding
T12972 7456-7458 NNP denotes CF
T12973 7459-7463 NN denotes cell
T12974 7464-7469 NNS denotes lines
T12975 7470-7471 -LRB- denotes (
T12976 7471-7472 -LRB- denotes (
T12977 7472-7473 NNP denotes B
T12978 7473-7474 -RRB- denotes )
T12979 7475-7480 NNP denotes IB3-1
T12980 7480-7481 , denotes ,
T12981 7482-7485 CC denotes and
T12982 7486-7487 -LRB- denotes (
T12983 7487-7488 NNP denotes D
T12984 7488-7489 -RRB- denotes )
T12985 7490-7496 NN denotes CuFi-1
T12986 7496-7497 -RRB- denotes )
T12987 7498-7503 NNS denotes cells
T12988 7504-7508 VBD denotes were
T12989 7509-7519 VBN denotes stimulated
T12990 7520-7524 IN denotes with
T12991 7525-7527 NNP denotes P.
T12992 7528-7538 NN denotes aeruginosa
T12993 7539-7540 -LRB- denotes (
T12994 7540-7543 NNP denotes MOI
T12995 7544-7545 SYM denotes =
T12996 7546-7548 CD denotes 10
T12997 7548-7549 -RRB- denotes )
T12998 7549-7550 , denotes ,
T12999 7551-7554 NNP denotes ATP
T13000 7555-7556 -LRB- denotes (
T13001 7556-7557 CD denotes 5
T13002 7558-7560 NNP denotes mM
T13003 7560-7561 -RRB- denotes )
T13004 7561-7562 , denotes ,
T13005 7563-7565 CC denotes or
T13006 7566-7571 NN denotes IL-1β
T13007 7572-7573 -LRB- denotes (
T13008 7573-7575 CD denotes 10
T13009 7576-7581 NN denotes ng/ml
T13010 7581-7582 -RRB- denotes )
T13011 7582-7583 , denotes ,
T13012 7584-7587 IN denotes for
T13013 7588-7591 DT denotes the
T13014 7592-7601 JJ denotes indicated
T13015 7602-7607 NNS denotes times
T13016 7608-7609 -LRB- denotes (
T13017 7609-7610 FW denotes n
T13018 7611-7612 SYM denotes =
T13019 7613-7614 CD denotes 3
T13020 7615-7625 JJ denotes individual
T13021 7626-7637 NNS denotes experiments
T13022 7637-7638 -RRB- denotes )
T13023 7638-7639 . denotes .
T13024 7640-7645 NNS denotes Cells
T13025 7646-7650 VBD denotes were
T13026 7651-7657 VBN denotes primed
T13027 7658-7662 IN denotes with
T13028 7663-7666 NNP denotes LPS
T13029 7667-7668 -LRB- denotes (
T13030 7668-7671 CD denotes 100
T13031 7672-7677 NN denotes ng/ml
T13032 7677-7678 -RRB- denotes )
T13033 7679-7682 IN denotes for
T13034 7683-7684 CD denotes 4
T13035 7685-7690 NNS denotes hours
T13036 7691-7696 WRB denotes where
T13037 7697-7708 JJ denotes appropriate
T13038 7708-7709 . denotes .
T13039 7710-7714 NNP denotes Cell
T13040 7715-7722 NN denotes culture
T13041 7723-7735 NNS denotes supernatants
T13042 7736-7740 VBD denotes were
T13043 7741-7748 VBN denotes assayed
T13044 7749-7752 IN denotes for
T13045 7753-7758 JJ denotes IL-1β
T13046 7759-7762 CC denotes and
T13047 7763-7767 JJ denotes IL-8
T13048 7768-7778 NN denotes production
T13049 7779-7781 IN denotes by
T13050 7782-7787 NNP denotes ELISA
T13051 7787-7788 . denotes .
T13052 7789-7795 VB denotes Insert
T13053 7796-7801 NNS denotes shows
T13054 7802-7806 JJ denotes IL-8
T13055 7807-7816 NN denotes secretion
T13056 7817-7819 IN denotes in
T13057 7820-7828 NN denotes response
T13058 7829-7831 TO denotes to
T13059 7832-7843 NN denotes stimulation
T13060 7844-7848 IN denotes with
T13061 7849-7854 JJ denotes IL-1β
T13062 7855-7856 -LRB- denotes (
T13063 7856-7858 CD denotes 10
T13064 7859-7864 NN denotes ng/ml
T13065 7864-7865 -RRB- denotes )
T13066 7865-7866 . denotes .
T13246 8724-8730 NNP denotes Airway
T13247 8731-8736 NNS denotes cells
T13248 8737-8739 VBP denotes do
T13249 8740-8743 RB denotes not
T13250 8744-8752 RB denotes strongly
T13251 8753-8763 VB denotes upregulate
T13252 8764-8773 JJ denotes caspase-1
T13253 8774-8782 NN denotes activity
T13254 8783-8785 IN denotes in
T13255 8786-8794 NN denotes response
T13256 8795-8797 TO denotes to
T13257 8798-8810 JJ denotes inflammasome
T13258 8811-8818 NNS denotes stimuli
T13259 8818-8819 . denotes .
T13260 8820-8822 CD denotes S9
T13261 8823-8826 CC denotes and
T13262 8827-8832 CD denotes IB3-1
T13263 8833-8838 NNS denotes cells
T13264 8839-8843 VBD denotes were
T13265 8844-8852 VBN denotes examined
T13266 8853-8856 IN denotes for
T13267 8857-8866 JJ denotes caspase-1
T13268 8867-8877 NN denotes activation
T13269 8878-8887 VBG denotes following
T13270 8888-8900 JJ denotes inflammasome
T13271 8901-8912 NN denotes stimulation
T13272 8913-8917 IN denotes with
T13273 8918-8920 NNP denotes P.
T13274 8921-8931 NN denotes aeruginosa
T13275 8932-8933 -LRB- denotes (
T13276 8933-8936 NNP denotes MOI
T13277 8937-8938 SYM denotes =
T13278 8939-8941 CD denotes 10
T13279 8941-8942 -RRB- denotes )
T13280 8943-8946 CC denotes and
T13281 8947-8950 NNP denotes ATP
T13282 8951-8952 -LRB- denotes (
T13283 8952-8953 CD denotes 5
T13284 8954-8956 NNP denotes mM
T13285 8956-8957 -RRB- denotes )
T13286 8957-8958 . denotes .
T13287 8959-8964 NNS denotes Cells
T13288 8965-8969 VBD denotes were
T13289 8970-8976 VBN denotes primed
T13290 8977-8981 IN denotes with
T13291 8982-8985 NNP denotes LPS
T13292 8986-8989 IN denotes for
T13293 8990-8991 CD denotes 5
T13294 8992-8997 NNS denotes hours
T13295 8998-9003 WRB denotes where
T13296 9004-9015 JJ denotes appropriate
T13297 9015-9016 . denotes .
T13298 9017-9018 DT denotes A
T13299 9019-9033 JJ denotes representative
T13300 9034-9043 NN denotes histogram
T13301 9044-9046 IN denotes of
T13302 9047-9048 NN denotes %
T13303 9049-9065 JJ denotes caspase-1-active
T13304 9066-9071 NNS denotes cells
T13305 9072-9074 VBZ denotes is
T13306 9075-9080 VBN denotes shown
T13307 9081-9083 IN denotes in
T13308 9084-9085 -LRB- denotes (
T13309 9085-9086 NN denotes A
T13310 9086-9087 -RRB- denotes )
T13311 9088-9091 CC denotes and
T13312 9092-9095 DT denotes the
T13313 9096-9104 VBD denotes averaged
T13314 9105-9111 NNS denotes values
T13315 9112-9115 VBP denotes are
T13316 9116-9121 VBN denotes shown
T13317 9122-9124 IN denotes in
T13318 9125-9126 -LRB- denotes (
T13319 9126-9127 NNP denotes B
T13320 9127-9128 -RRB- denotes )
T13321 9129-9130 -LRB- denotes (
T13322 9130-9131 FW denotes n
T13323 9132-9133 SYM denotes =
T13324 9134-9135 CD denotes 3
T13325 9136-9144 JJ denotes separate
T13326 9145-9156 NNS denotes experiments
T13327 9156-9157 -RRB- denotes )
T13328 9157-9158 . denotes .
T13329 9159-9160 -LRB- denotes (
T13330 9160-9161 $ denotes C
T13331 9161-9162 -RRB- denotes )
T13332 9163-9168 JJ denotes IB3-1
T13333 9169-9174 NNS denotes cells
T13334 9175-9176 -LRB- denotes (
T13335 9176-9177 CD denotes 5
T13336 9177-9178 CD denotes ×
T13337 9178-9181 CD denotes 104
T13338 9182-9192 NN denotes cells/well
T13339 9192-9193 -RRB- denotes )
T13340 9194-9198 VBD denotes were
T13341 9199-9210 JJ denotes pre-treated
T13342 9211-9214 IN denotes for
T13343 9215-9216 CD denotes 1
T13344 9217-9221 NN denotes hour
T13345 9222-9226 IN denotes with
T13346 9227-9237 VBG denotes increasing
T13347 9238-9252 NNS denotes concentrations
T13348 9253-9255 IN denotes of
T13349 9256-9266 NNP denotes z-YVAD-fmk
T13350 9267-9268 -LRB- denotes (
T13351 9268-9270 CD denotes 10
T13352 9271-9273 CD denotes 30
T13353 9274-9276 NNP denotes µM
T13354 9276-9277 -RRB- denotes )
T13355 9278-9283 RB denotes prior
T13356 9284-9286 TO denotes to
T13357 9287-9298 NN denotes stimulation
T13358 9299-9303 IN denotes with
T13359 9304-9306 NNP denotes P.
T13360 9307-9317 NN denotes aeruginosa
T13361 9318-9319 -LRB- denotes (
T13362 9319-9322 NNP denotes MOI
T13363 9323-9324 SYM denotes =
T13364 9325-9327 CD denotes 50
T13365 9327-9328 -RRB- denotes )
T13366 9328-9329 . denotes .
T13367 9330-9334 NNP denotes Cell
T13368 9335-9342 NN denotes culture
T13369 9343-9355 NNS denotes supernatants
T13370 9356-9360 VBD denotes were
T13371 9361-9370 VBN denotes collected
T13372 9371-9376 IN denotes after
T13373 9377-9378 CD denotes 6
T13374 9379-9384 NNS denotes hours
T13375 9385-9388 CC denotes and
T13376 9389-9396 VBN denotes assayed
T13377 9397-9400 IN denotes for
T13378 9401-9405 NN denotes IL-6
T13379 9406-9408 IN denotes by
T13380 9409-9414 NNP denotes ELISA
T13381 9415-9416 -LRB- denotes (
T13382 9416-9417 FW denotes n
T13383 9418-9419 SYM denotes =
T13384 9420-9421 LS denotes 3
T13385 9421-9422 -RRB- denotes )
T13386 9422-9423 . denotes .
T13509 9944-9949 NNS denotes PBMCs
T13510 9950-9954 IN denotes from
T13511 9955-9957 NNP denotes CF
T13512 9958-9966 NNS denotes patients
T13513 9967-9970 CC denotes and
T13514 9971-9979 NNS denotes controls
T13515 9980-9984 VBP denotes show
T13516 9985-9992 JJ denotes similar
T13517 9993-10002 NNS denotes increases
T13518 10003-10005 IN denotes in
T13519 10006-10015 JJ denotes caspase-1
T13520 10016-10024 NN denotes activity
T13521 10025-10029 IN denotes upon
T13522 10030-10042 JJ denotes inflammasome
T13523 10043-10053 NN denotes activation
T13524 10053-10054 . denotes .
T13525 10055-10060 NNS denotes PBMCs
T13526 10061-10065 IN denotes from
T13527 10066-10068 NNP denotes CF
T13528 10069-10077 NNS denotes patients
T13529 10078-10079 -LRB- denotes (
T13530 10079-10080 FW denotes n
T13531 10081-10082 SYM denotes =
T13532 10083-10084 CD denotes 6
T13533 10084-10085 -RRB- denotes )
T13534 10086-10089 CC denotes and
T13535 10090-10097 JJ denotes healthy
T13536 10098-10106 NNS denotes controls
T13537 10107-10108 -LRB- denotes (
T13538 10108-10109 FW denotes n
T13539 10110-10111 SYM denotes =
T13540 10112-10113 CD denotes 6
T13541 10113-10114 -RRB- denotes )
T13542 10115-10119 VBD denotes were
T13543 10120-10126 VBN denotes primed
T13544 10127-10131 IN denotes with
T13545 10132-10135 NNP denotes LPS
T13546 10136-10137 -LRB- denotes (
T13547 10137-10139 CD denotes 10
T13548 10140-10145 NN denotes ng/ml
T13549 10145-10146 -RRB- denotes )
T13550 10147-10150 IN denotes for
T13551 10151-10152 CD denotes 5
T13552 10153-10158 NNS denotes hours
T13553 10159-10164 RB denotes prior
T13554 10165-10167 TO denotes to
T13555 10168-10179 NN denotes stimulation
T13556 10180-10184 IN denotes with
T13557 10185-10188 NNP denotes ATP
T13558 10189-10190 -LRB- denotes (
T13559 10190-10191 CD denotes 5
T13560 10192-10194 NNP denotes mM
T13561 10194-10195 -RRB- denotes )
T13562 10196-10199 IN denotes for
T13563 10200-10201 CD denotes 1
T13564 10202-10206 NN denotes hour
T13565 10207-10209 CC denotes or
T13566 10210-10214 NNP denotes Poly
T13567 10214-10215 -LRB- denotes (
T13568 10215-10217 NNP denotes dA
T13569 10217-10218 : denotes :
T13570 10218-10220 NN denotes dT
T13571 10220-10221 -RRB- denotes )
T13572 10222-10223 -LRB- denotes (
T13573 10223-10224 CD denotes 1
T13574 10225-10227 NN denotes µg
T13575 10227-10228 NN denotes /
T13576 10228-10230 NN denotes ml
T13577 10230-10231 -RRB- denotes )
T13578 10232-10235 IN denotes for
T13579 10236-10237 CD denotes 3
T13580 10238-10243 NNS denotes hours
T13581 10243-10244 . denotes .
T13582 10245-10250 NNS denotes PBMCs
T13583 10251-10255 VBD denotes were
T13584 10256-10266 VBN denotes stimulated
T13585 10267-10271 IN denotes with
T13586 10272-10274 NNP denotes P.
T13587 10275-10285 NN denotes aeruginosa
T13588 10286-10292 NN denotes strain
T13589 10293-10297 NNP denotes PAO1
T13590 10298-10301 IN denotes for
T13591 10302-10303 CD denotes 3
T13592 10304-10309 NNS denotes hours
T13593 10309-10310 . denotes .
T13594 10311-10312 DT denotes A
T13595 10313-10327 JJ denotes representative
T13596 10328-10337 NN denotes histogram
T13597 10338-10340 IN denotes of
T13598 10341-10344 DT denotes the
T13599 10345-10346 NN denotes %
T13600 10347-10363 JJ denotes caspase-1-active
T13601 10364-10369 NNS denotes cells
T13602 10370-10372 VBZ denotes is
T13603 10373-10378 VBN denotes shown
T13604 10379-10381 IN denotes in
T13605 10382-10383 -LRB- denotes (
T13606 10383-10384 NN denotes A
T13607 10384-10385 -RRB- denotes )
T13608 10386-10390 IN denotes with
T13609 10391-10394 DT denotes the
T13610 10395-10403 VBN denotes averaged
T13611 10404-10410 NNS denotes values
T13612 10411-10416 VBN denotes shown
T13613 10417-10419 IN denotes in
T13614 10420-10421 -LRB- denotes (
T13615 10421-10422 NNP denotes B
T13616 10422-10423 -RRB- denotes )
T13617 10423-10424 . denotes .
T13753 11893-11898 NNS denotes PBMCs
T13754 11899-11903 IN denotes from
T13755 11904-11906 NNP denotes CF
T13756 11907-11915 NNS denotes patients
T13757 11916-11918 VBP denotes do
T13758 11919-11922 RB denotes not
T13759 11923-11930 VB denotes produce
T13760 11931-11940 JJ denotes increased
T13761 11941-11948 NNS denotes amounts
T13762 11949-11951 IN denotes of
T13763 11952-11957 NN denotes IL-1β
T13764 11957-11958 . denotes .
T13765 11959-11964 NNS denotes PBMCs
T13766 11965-11969 IN denotes from
T13767 11970-11972 NNP denotes CF
T13768 11973-11981 NNS denotes patients
T13769 11982-11983 -LRB- denotes (
T13770 11983-11984 FW denotes n
T13771 11985-11986 SYM denotes =
T13772 11987-11989 CD denotes 17
T13773 11990-11992 CD denotes 20
T13774 11992-11993 -RRB- denotes )
T13775 11994-11997 CC denotes and
T13776 11998-12005 JJ denotes healthy
T13777 12006-12014 NNS denotes controls
T13778 12015-12016 -LRB- denotes (
T13779 12016-12017 FW denotes n
T13780 12018-12019 SYM denotes =
T13781 12020-12022 CD denotes 15
T13782 12023-12025 CD denotes 19
T13783 12025-12026 -RRB- denotes )
T13784 12027-12031 VBD denotes were
T13785 12032-12038 VBN denotes primed
T13786 12039-12043 IN denotes with
T13787 12044-12047 NNP denotes LPS
T13788 12048-12049 -LRB- denotes (
T13789 12049-12051 CD denotes 10
T13790 12052-12057 NN denotes ng/ml
T13791 12057-12058 -RRB- denotes )
T13792 12059-12068 JJ denotes overnight
T13793 12069-12072 CC denotes and
T13794 12073-12083 VBN denotes stimulated
T13795 12084-12088 IN denotes with
T13796 12089-12091 NNP denotes P.
T13797 12092-12102 NN denotes aeruginosa
T13798 12103-12107 NNP denotes PAO1
T13799 12108-12109 -LRB- denotes (
T13800 12109-12112 NNP denotes MOI
T13801 12113-12114 SYM denotes =
T13802 12115-12116 LS denotes 1
T13803 12116-12117 -RRB- denotes )
T13804 12117-12118 , denotes ,
T13805 12119-12121 NNP denotes P.
T13806 12122-12132 NNP denotes aeruginosa
T13807 12133-12137 NNP denotes PAO1
T13808 12138-12145 VBG denotes lacking
T13809 12146-12150 NNP denotes exsA
T13810 12151-12152 -LRB- denotes (
T13811 12152-12155 NNP denotes MOI
T13812 12156-12157 SYM denotes =
T13813 12158-12159 LS denotes 1
T13814 12159-12160 -RRB- denotes )
T13815 12160-12161 , denotes ,
T13816 12162-12165 NNP denotes ATP
T13817 12166-12167 -LRB- denotes (
T13818 12167-12168 CD denotes 5
T13819 12169-12171 NNP denotes mM
T13820 12171-12172 -RRB- denotes )
T13821 12172-12173 , denotes ,
T13822 12174-12176 CC denotes or
T13823 12177-12181 NNP denotes Poly
T13824 12181-12182 -LRB- denotes (
T13825 12182-12184 NNP denotes dA
T13826 12184-12185 : denotes :
T13827 12185-12187 NN denotes dT
T13828 12187-12188 -RRB- denotes )
T13829 12189-12190 -LRB- denotes (
T13830 12190-12191 CD denotes 1
T13831 12192-12194 NN denotes µg
T13832 12194-12195 NN denotes /
T13833 12195-12197 NN denotes ml
T13834 12197-12198 -RRB- denotes )
T13835 12199-12202 IN denotes for
T13836 12203-12205 CD denotes 24
T13837 12206-12211 NNS denotes hours
T13838 12211-12212 . denotes .
T13839 12213-12215 NNP denotes P.
T13840 12216-12226 NN denotes aeruginosa
T13841 12227-12234 VBG denotes lacking
T13842 12235-12239 NNP denotes exsA
T13843 12240-12243 VBD denotes was
T13844 12244-12248 VBN denotes used
T13845 12249-12251 IN denotes as
T13846 12252-12253 DT denotes a
T13847 12254-12258 NN denotes type
T13848 12259-12262 NNP denotes III
T13849 12263-12272 NN denotes secretion
T13850 12273-12280 NN denotes control
T13851 12281-12283 IN denotes in
T13852 12284-12294 NN denotes comparison
T13853 12295-12299 IN denotes with
T13854 12300-12309 JJ denotes wild-type
T13855 12310-12312 NNP denotes P.
T13856 12313-12323 NN denotes aeruginosa
T13857 12323-12324 . denotes .
T13858 12325-12337 NNS denotes Supernatants
T13859 12338-12342 VBD denotes were
T13860 12343-12350 VBN denotes assayed
T13861 12351-12354 IN denotes for
T13862 12355-12356 -LRB- denotes (
T13863 12356-12357 NN denotes A
T13864 12357-12358 -RRB- denotes )
T13865 12359-12364 NN denotes IL-1β
T13866 12365-12368 CC denotes and
T13867 12369-12370 -LRB- denotes (
T13868 12370-12371 NNP denotes B
T13869 12371-12372 -RRB- denotes )
T13870 12373-12377 NN denotes IL-8
T13871 12377-12378 . denotes .
T14178 14091-14096 JJ denotes NF-κB
T14179 14097-14107 NN denotes activation
T14180 14108-14119 VBZ denotes potentiates
T14181 14120-14123 DT denotes the
T14182 14124-14130 NN denotes degree
T14183 14131-14133 IN denotes of
T14184 14134-14139 JJ denotes IL-1β
T14185 14140-14150 NN denotes production
T14186 14151-14154 CC denotes and
T14187 14155-14164 NN denotes secretion
T14188 14165-14169 IN denotes upon
T14189 14170-14182 JJ denotes inflammasome
T14190 14183-14193 NN denotes activation
T14191 14193-14194 . denotes .
T14192 14195-14200 CD denotes THP-1
T14193 14201-14209 NN denotes reporter
T14194 14210-14215 NNS denotes cells
T14195 14216-14220 VBD denotes were
T14196 14221-14227 VBN denotes primed
T14197 14228-14237 JJ denotes overnight
T14198 14238-14242 IN denotes with
T14199 14243-14254 JJ denotes heat-killed
T14200 14255-14257 NNP denotes P.
T14201 14258-14268 NN denotes aeruginosa
T14202 14269-14272 CC denotes and
T14203 14273-14283 VBD denotes stimulated
T14204 14284-14287 DT denotes the
T14205 14288-14292 JJ denotes next
T14206 14293-14296 NN denotes day
T14207 14297-14301 IN denotes with
T14208 14302-14306 JJ denotes live
T14209 14307-14309 NNP denotes P.
T14210 14310-14320 NN denotes aeruginosa
T14211 14321-14323 CC denotes or
T14212 14324-14334 JJ denotes additional
T14213 14335-14346 JJ denotes heat-killed
T14214 14347-14349 NNP denotes P.
T14215 14350-14360 NN denotes aeruginosa
T14216 14361-14364 IN denotes for
T14217 14365-14368 DT denotes the
T14218 14369-14374 NNS denotes times
T14219 14375-14384 VBD denotes indicated
T14220 14384-14385 . denotes .
T14221 14386-14390 NNP denotes Cell
T14222 14391-14398 NN denotes culture
T14223 14399-14411 NNS denotes supernatants
T14224 14412-14416 VBD denotes were
T14225 14417-14424 VBN denotes assayed
T14226 14425-14428 IN denotes for
T14227 14429-14430 -LRB- denotes (
T14228 14430-14431 NN denotes A
T14229 14431-14432 -RRB- denotes )
T14230 14433-14438 NN denotes NF-κB
T14231 14438-14439 NN denotes /
T14232 14439-14443 NN denotes AP-1
T14233 14444-14452 NN denotes activity
T14234 14452-14453 , denotes ,
T14235 14454-14455 -LRB- denotes (
T14236 14455-14456 NNP denotes B
T14237 14456-14457 -RRB- denotes )
T14238 14458-14462 NN denotes IL-8
T14239 14462-14463 , denotes ,
T14240 14464-14467 CC denotes and
T14241 14468-14469 -LRB- denotes (
T14242 14469-14470 NNP denotes C
T14243 14470-14471 -RRB- denotes )
T14244 14472-14477 JJ denotes IL-1β
T14245 14478-14487 NN denotes secretion
T14246 14488-14489 -LRB- denotes (
T14247 14489-14490 FW denotes n
T14248 14491-14492 SYM denotes =
T14249 14493-14494 CD denotes 3
T14250 14495-14496 CD denotes 6
T14251 14497-14508 NNS denotes experiments
T14252 14508-14509 -RRB- denotes )
T14253 14509-14510 . denotes .
T14254 14511-14516 VBG denotes Using
T14255 14517-14520 DT denotes the
T14256 14521-14525 JJ denotes same
T14257 14526-14537 NN denotes stimulation
T14258 14538-14544 NN denotes method
T14259 14544-14545 , denotes ,
T14260 14546-14551 CD denotes THP-1
T14261 14552-14560 NN denotes reporter
T14262 14561-14566 NNS denotes cells
T14263 14567-14571 VBD denotes were
T14264 14572-14579 VBN denotes treated
T14265 14580-14584 IN denotes with
T14266 14585-14595 NN denotes Bay11-7082
T14267 14596-14597 -LRB- denotes (
T14268 14597-14599 CD denotes 20
T14269 14600-14602 NNP denotes µM
T14270 14602-14603 -RRB- denotes )
T14271 14604-14607 IN denotes for
T14272 14608-14609 CD denotes 1
T14273 14610-14614 NN denotes hour
T14274 14615-14620 RB denotes prior
T14275 14621-14623 TO denotes to
T14276 14624-14631 VBG denotes priming
T14277 14632-14636 IN denotes with
T14278 14637-14648 JJ denotes heat-killed
T14279 14649-14653 NNP denotes PAO1
T14280 14654-14656 CC denotes or
T14281 14657-14661 JJ denotes live
T14282 14662-14666 NN denotes PAO1
T14283 14666-14667 . denotes .
T14284 14668-14680 NNS denotes Supernatants
T14285 14681-14685 VBD denotes were
T14286 14686-14693 VBN denotes assayed
T14287 14694-14696 IN denotes at
T14288 14697-14699 CD denotes 24
T14289 14700-14705 NNS denotes hours
T14290 14706-14709 IN denotes for
T14291 14710-14711 -LRB- denotes (
T14292 14711-14712 NNP denotes D
T14293 14712-14713 -RRB- denotes )
T14294 14714-14719 NNP denotes NF-κB
T14295 14719-14720 NNP denotes /
T14296 14720-14724 NNP denotes AP-1
T14297 14725-14733 NN denotes activity
T14298 14733-14734 , denotes ,
T14299 14735-14736 -LRB- denotes (
T14300 14736-14737 NNP denotes E
T14301 14737-14738 -RRB- denotes )
T14302 14739-14743 NN denotes IL-8
T14303 14743-14744 , denotes ,
T14304 14745-14748 CC denotes and
T14305 14749-14750 -LRB- denotes (
T14306 14750-14751 NN denotes F
T14307 14751-14752 -RRB- denotes )
T14308 14753-14758 NN denotes IL-1β
T14309 14759-14768 NN denotes secretion
T14310 14769-14770 -LRB- denotes (
T14311 14770-14771 FW denotes n
T14312 14772-14773 SYM denotes =
T14313 14774-14775 CD denotes 3
T14314 14776-14777 LS denotes 5
T14315 14777-14778 -RRB- denotes )
T14316 14778-14779 . denotes .
T14317 14780-14785 NNS denotes PBMCs
T14318 14786-14790 IN denotes from
T14319 14791-14793 NNP denotes CF
T14320 14794-14802 NNS denotes patients
T14321 14803-14804 -LRB- denotes (
T14322 14804-14805 FW denotes n
T14323 14806-14807 SYM denotes =
T14324 14808-14810 CD denotes 11
T14325 14811-14813 CD denotes 15
T14326 14813-14814 -RRB- denotes )
T14327 14815-14818 CC denotes and
T14328 14819-14827 NNS denotes controls
T14329 14828-14829 -LRB- denotes (
T14330 14829-14830 FW denotes n
T14331 14831-14832 SYM denotes =
T14332 14833-14835 CD denotes 10
T14333 14836-14838 CD denotes 13
T14334 14838-14839 -RRB- denotes )
T14335 14840-14844 VBD denotes were
T14336 14845-14852 VBN denotes treated
T14337 14853-14857 IN denotes with
T14338 14858-14868 NNP denotes z-YVAD-fmk
T14339 14869-14870 -LRB- denotes (
T14340 14870-14872 CD denotes 20
T14341 14873-14875 NNP denotes µM
T14342 14875-14876 -RRB- denotes )
T14343 14877-14879 CC denotes or
T14344 14880-14890 NN denotes Bay11-7082
T14345 14891-14892 -LRB- denotes (
T14346 14892-14894 CD denotes 10
T14347 14895-14897 NNP denotes µM
T14348 14897-14898 -RRB- denotes )
T14349 14899-14902 CC denotes and
T14350 14903-14913 VBN denotes stimulated
T14351 14914-14918 IN denotes with
T14352 14919-14923 JJ denotes live
T14353 14924-14928 NNP denotes PAO1
T14354 14929-14930 -LRB- denotes (
T14355 14930-14933 NNP denotes MOI
T14356 14934-14935 SYM denotes =
T14357 14936-14937 LS denotes 1
T14358 14937-14938 -RRB- denotes )
T14359 14938-14939 , denotes ,
T14360 14940-14943 NNP denotes ATP
T14361 14944-14945 -LRB- denotes (
T14362 14945-14946 CD denotes 5
T14363 14947-14949 NNP denotes mM
T14364 14949-14950 -RRB- denotes )
T14365 14950-14951 , denotes ,
T14366 14952-14954 CC denotes or
T14367 14955-14959 NNP denotes Poly
T14368 14959-14960 -LRB- denotes (
T14369 14960-14962 NNP denotes dA
T14370 14962-14963 : denotes :
T14371 14963-14965 NN denotes dT
T14372 14965-14966 -RRB- denotes )
T14373 14967-14968 -LRB- denotes (
T14374 14968-14969 CD denotes 1
T14375 14970-14972 NN denotes µg
T14376 14972-14973 NN denotes /
T14377 14973-14975 NN denotes ml
T14378 14975-14976 -RRB- denotes )
T14379 14977-14986 VBG denotes according
T14380 14987-14989 TO denotes to
T14381 14990-14993 DT denotes the
T14382 14994-15002 NN denotes schedule
T14383 15003-15005 IN denotes in
T14384 15006-15012 NN denotes Figure
T14385 15013-15014 CD denotes 1
T14386 15014-15015 . denotes .
T14387 15016-15017 -LRB- denotes (
T14388 15017-15018 NNP denotes G
T14389 15018-15019 -RRB- denotes )
T14390 15020-15025 NN denotes IL-1β
T14391 15026-15029 CC denotes and
T14392 15030-15031 -LRB- denotes (
T14393 15031-15032 NNP denotes H
T14394 15032-15033 -RRB- denotes )
T14395 15034-15038 NN denotes IL-8
T14396 15039-15045 NNS denotes levels
T14397 15046-15050 VBD denotes were
T14398 15051-15059 VBN denotes measured
T14399 15060-15062 IN denotes at
T14400 15063-15065 CD denotes 24
T14401 15066-15071 NNS denotes hours
T14402 15071-15072 . denotes .
T14403 15073-15084 NNP denotes Statistical
T14404 15085-15093 NN denotes analysis
T14405 15094-15097 VBD denotes was
T14406 15098-15107 VBN denotes performed
T14407 15108-15113 VBG denotes using
T14408 15114-15117 CD denotes two
T14409 15118-15121 NN denotes way
T14410 15122-15127 NNP denotes ANOVA
T14411 15128-15132 IN denotes with
T14412 15133-15143 NNP denotes Bonferroni
T14413 15144-15154 NN denotes correction
T14414 15155-15158 IN denotes for
T14415 15159-15167 JJ denotes multiple
T14416 15168-15179 NNS denotes comparisons
T14417 15179-15180 . denotes .
T14418 15181-15182 SYM denotes *
T14419 15182-15183 , denotes ,
T14420 15184-15186 SYM denotes **
T14421 15186-15187 , denotes ,
T14422 15188-15191 CC denotes and
T14423 15192-15195 RB denotes ***
T14424 15196-15203 VB denotes signify
T14425 15204-15205 NNP denotes P
T14426 15205-15206 CD denotes <
T14427 15206-15210 CD denotes 0.05
T14428 15210-15211 , denotes ,
T14429 15212-15216 CD denotes 0.01
T14430 15216-15217 , denotes ,
T14431 15218-15221 CC denotes and
T14432 15222-15227 CD denotes 0.001
T14433 15227-15228 . denotes .
T14573 15927-15937 JJ denotes Bay11-7082
T14574 15938-15946 NNS denotes inhibits
T14575 15947-15956 JJ denotes pro-IL-1β
T14576 15957-15967 NN denotes production
T14577 15968-15970 IN denotes in
T14578 15971-15979 NN denotes response
T14579 15980-15982 TO denotes to
T14580 15983-15985 NNP denotes P.
T14581 15986-15996 NN denotes aeruginosa
T14582 15996-15997 . denotes .
T14583 15998-16016 JJ denotes PMA-differentiated
T14584 16017-16022 NN denotes THP-1
T14585 16023-16028 NNS denotes cells
T14586 16029-16033 VBD denotes were
T14587 16034-16041 VBN denotes treated
T14588 16042-16046 IN denotes with
T14589 16047-16049 CD denotes 10
T14590 16050-16052 NNP denotes µM
T14591 16053-16063 NNP denotes CFTRinh172
T14592 16064-16066 CC denotes or
T14593 16067-16069 CD denotes 20
T14594 16070-16072 NNP denotes µM
T14595 16073-16083 NNP denotes Bay11-7082
T14596 16084-16087 CC denotes and
T14597 16088-16097 VBN denotes harvested
T14598 16098-16103 IN denotes after
T14599 16104-16105 -LRB- denotes (
T14600 16105-16106 NN denotes A
T14601 16106-16107 -RRB- denotes )
T14602 16108-16109 CD denotes 4
T14603 16110-16115 NNS denotes hours
T14604 16116-16117 -LRB- denotes (
T14605 16117-16118 FW denotes n
T14606 16119-16120 SYM denotes =
T14607 16121-16122 LS denotes 3
T14608 16122-16123 -RRB- denotes )
T14609 16124-16126 CC denotes or
T14610 16127-16128 -LRB- denotes (
T14611 16128-16129 NNP denotes B
T14612 16129-16130 -RRB- denotes )
T14613 16131-16134 CD denotes 0.5
T14614 16134-16135 , denotes ,
T14615 16136-16137 CD denotes 1
T14616 16137-16138 , denotes ,
T14617 16139-16142 CC denotes and
T14618 16143-16146 CD denotes 1.5
T14619 16147-16152 NNS denotes hours
T14620 16153-16154 -LRB- denotes (
T14621 16154-16155 FW denotes n
T14622 16156-16157 SYM denotes =
T14623 16158-16159 LS denotes 3
T14624 16159-16160 -RRB- denotes )
T14625 16161-16172 NN denotes stimulation
T14626 16173-16177 IN denotes with
T14627 16178-16182 NNP denotes PAO1
T14628 16182-16183 . denotes .
T14629 16184-16187 CD denotes One
T14630 16188-16202 NN denotes representative
T14631 16203-16207 NN denotes blot
T14632 16208-16210 VBZ denotes is
T14633 16211-16216 VBN denotes shown
T14634 16217-16221 IN denotes with
T14635 16222-16223 DT denotes a
T14636 16224-16229 NN denotes graph
T14637 16230-16232 IN denotes of
T14638 16233-16236 DT denotes the
T14639 16237-16245 VBN denotes averaged
T14640 16246-16258 NN denotes fluorescence
T14641 16259-16268 NN denotes intensity
T14642 16269-16275 NNS denotes values
T14643 16276-16280 IN denotes over
T14644 16281-16282 CD denotes 3
T14645 16283-16294 NNS denotes experiments
T14646 16294-16295 . denotes .
T14813 17130-17140 NN denotes Disruption
T14814 17141-17143 IN denotes of
T14815 17144-17148 NNP denotes CFTR
T14816 17149-17157 NN denotes activity
T14817 17158-17162 VBZ denotes does
T14818 17163-17166 RB denotes not
T14819 17167-17175 VB denotes increase
T14820 17176-17181 JJ denotes IL-1β
T14821 17182-17192 NN denotes production
T14822 17193-17195 IN denotes in
T14823 17196-17201 NNP denotes PBMCs
T14824 17202-17204 CC denotes or
T14825 17205-17216 NNS denotes macrophages
T14826 17216-17217 . denotes .
T14827 17218-17223 NNS denotes PBMCs
T14828 17224-17228 IN denotes from
T14829 17229-17231 NNP denotes CF
T14830 17232-17240 NNS denotes patients
T14831 17241-17242 -LRB- denotes (
T14832 17242-17243 FW denotes n
T14833 17244-17245 SYM denotes =
T14834 17246-17248 CD denotes 15
T14835 17248-17249 -RRB- denotes )
T14836 17250-17253 CC denotes and
T14837 17254-17262 NNS denotes controls
T14838 17263-17264 -LRB- denotes (
T14839 17264-17265 FW denotes n
T14840 17266-17267 SYM denotes =
T14841 17268-17270 CD denotes 13
T14842 17270-17271 -RRB- denotes )
T14843 17272-17276 VBD denotes were
T14844 17277-17284 VBN denotes treated
T14845 17285-17289 IN denotes with
T14846 17290-17300 NNP denotes CFTRinh172
T14847 17301-17302 -LRB- denotes (
T14848 17302-17304 CD denotes 10
T14849 17305-17307 NNP denotes µM
T14850 17307-17308 -RRB- denotes )
T14851 17309-17312 IN denotes for
T14852 17313-17315 CD denotes 18
T14853 17316-17321 NNS denotes hours
T14854 17322-17327 RB denotes prior
T14855 17328-17330 TO denotes to
T14856 17331-17342 NN denotes stimulation
T14857 17343-17347 IN denotes with
T14858 17348-17352 JJ denotes live
T14859 17353-17357 NNP denotes PAO1
T14860 17358-17359 -LRB- denotes (
T14861 17359-17362 NNP denotes MOI
T14862 17363-17364 SYM denotes =
T14863 17365-17366 LS denotes 1
T14864 17366-17367 -RRB- denotes )
T14865 17367-17368 . denotes .
T14866 17369-17370 -LRB- denotes (
T14867 17370-17371 NN denotes A
T14868 17371-17372 -RRB- denotes )
T14869 17373-17378 NN denotes IL-1β
T14870 17379-17382 CC denotes and
T14871 17383-17384 -LRB- denotes (
T14872 17384-17385 NNP denotes B
T14873 17385-17386 -RRB- denotes )
T14874 17387-17391 JJ denotes IL-8
T14875 17392-17402 NN denotes production
T14876 17403-17406 VBD denotes was
T14877 17407-17415 VBN denotes measured
T14878 17416-17418 IN denotes at
T14879 17419-17421 CD denotes 24
T14880 17422-17427 NNS denotes hours
T14881 17427-17428 . denotes .
T14882 17429-17430 -LRB- denotes (
T14883 17430-17431 $ denotes C
T14884 17431-17432 -RRB- denotes )
T14885 17433-17442 NNPS denotes Monocytes
T14886 17443-17447 IN denotes from
T14887 17448-17456 NNS denotes controls
T14888 17457-17458 -LRB- denotes (
T14889 17458-17459 FW denotes n
T14890 17460-17461 SYM denotes =
T14891 17462-17463 LS denotes 3
T14892 17463-17464 -RRB- denotes )
T14893 17465-17469 VBD denotes were
T14894 17470-17484 VBN denotes differentiated
T14895 17485-17489 IN denotes into
T14896 17490-17501 NNS denotes macrophages
T14897 17501-17502 . denotes .
T14898 17503-17514 NNS denotes Macrophages
T14899 17515-17519 VBD denotes were
T14900 17520-17527 VBN denotes treated
T14901 17528-17532 IN denotes with
T14902 17533-17543 NNP denotes CFTRinh172
T14903 17543-17544 , denotes ,
T14904 17545-17555 VBD denotes stimulated
T14905 17556-17558 IN denotes as
T14906 17559-17562 IN denotes per
T14907 17563-17572 NNS denotes monocytes
T14908 17572-17573 , denotes ,
T14909 17574-17577 CC denotes and
T14910 17578-17586 VBN denotes measured
T14911 17587-17590 IN denotes for
T14912 17591-17596 JJ denotes IL-1β
T14913 17597-17607 NN denotes production
T14914 17608-17610 IN denotes at
T14915 17611-17613 CD denotes 24
T14916 17614-17619 NNS denotes hours
T14917 17619-17620 . denotes .
T14918 17621-17626 CD denotes THP-1
T14919 17627-17635 NN denotes reporter
T14920 17636-17641 NNS denotes cells
T14921 17642-17646 VBD denotes were
T14922 17647-17654 VBN denotes treated
T14923 17655-17659 IN denotes with
T14924 17660-17670 NNP denotes CFTRinh172
T14925 17671-17673 CD denotes 24
T14926 17674-17679 NNS denotes hours
T14927 17680-17685 RB denotes prior
T14928 17686-17688 TO denotes to
T14929 17689-17700 NN denotes stimulation
T14930 17701-17705 IN denotes with
T14931 17706-17710 NNP denotes PAO1
T14932 17711-17714 CC denotes and
T14933 17715-17723 VBN denotes measured
T14934 17724-17727 IN denotes for
T14935 17728-17729 -LRB- denotes (
T14936 17729-17730 NNP denotes D
T14937 17730-17731 -RRB- denotes )
T14938 17732-17737 JJ denotes IL-1β
T14939 17738-17747 NN denotes secretion
T14940 17747-17748 , denotes ,
T14941 17749-17750 -LRB- denotes (
T14942 17750-17751 NNP denotes E
T14943 17751-17752 -RRB- denotes )
T14944 17753-17757 NN denotes IL-8
T14945 17757-17758 , denotes ,
T14946 17759-17762 CC denotes and
T14947 17763-17764 -LRB- denotes (
T14948 17764-17765 NN denotes F
T14949 17765-17766 -RRB- denotes )
T14950 17767-17772 NN denotes NF-κB
T14951 17772-17773 NN denotes /
T14952 17773-17777 NN denotes AP-1
T14953 17778-17786 NN denotes activity
T14954 17787-17789 IN denotes at
T14955 17790-17792 CD denotes 24
T14956 17793-17798 NNS denotes hours
T14957 17799-17800 -LRB- denotes (
T14958 17800-17801 FW denotes n
T14959 17802-17803 SYM denotes =
T14960 17804-17805 LS denotes 4
T14961 17805-17806 -RRB- denotes )
T14962 17806-17807 . denotes .
T15016 7910-7922 NNP denotes Inflammasome
T15017 7923-7933 NNP denotes Activators
T15018 7933-7934 . denotes .
R316 T378 T380 compound Inflammasome-Mediated,Production
R317 T379 T380 compound IL-1β,Production
R318 T380 T390 nsubj Production,are
R319 T381 T380 prep in,Production
R320 T382 T381 pobj Humans,in
R321 T383 T380 prep with,Production
R322 T384 T387 amod Cystic,Inflammation
R323 T385 T387 compound Fibrosis,Inflammation
R324 T386 T387 compound Background,Inflammation
R325 T387 T383 pobj Inflammation,with
R326 T388 T387 cc and,Inflammation
R327 T389 T387 conj infection,Inflammation
R328 T390 T390 ROOT are,are
R329 T391 T392 amod major,determinants
R330 T392 T390 attr determinants,are
R331 T393 T392 prep of,determinants
R332 T394 T395 compound disease,severity
R333 T395 T393 pobj severity,of
R334 T396 T390 cc and,are
R335 T397 T400 advmod consequently,quality
R336 T398 T400 punct ",",quality
R412 T474 T475 aux to,produce
R413 T475 T473 xcomp produce,found
R414 T476 T477 amod negligible,amounts
R415 T477 T475 dobj amounts,produce
R416 T478 T477 prep of,amounts
R417 T479 T478 pobj basal,of
R418 T480 T477 cc or,amounts
R419 T481 T482 amod stimulated,IL-1β
R420 T482 T477 conj IL-1β,amounts
R421 T483 T473 prep compared,found
R422 T484 T483 prep to,compared
R423 T485 T486 amod hematopoeitic,cells
R424 T486 T484 pobj cells,to
R425 T487 T473 cc and,found
R426 T488 T492 nsubj they,upregulate
R427 T489 T492 aux did,upregulate
R428 T490 T492 neg not,upregulate
R429 T491 T492 advmod significantly,upregulate
R430 T492 T473 conj upregulate,found
R431 T493 T494 amod caspase-1,activity
R432 T494 T492 dobj activity,upregulate
R433 T495 T492 prep upon,upregulate
R434 T496 T497 amod inflammasome,stimulation
R435 T497 T495 pobj stimulation,upon
R436 T498 T452 punct .,examines
R437 T499 T516 prep In,produced
R438 T500 T499 pobj contrast,In
R439 T501 T516 punct ",",produced
R440 T502 T505 amod peripheral,cells
R441 T503 T504 compound blood,mononuclear
R442 T504 T505 compound mononuclear,cells
R443 T505 T516 nsubj cells,produced
R444 T506 T505 punct (,cells
R445 T507 T505 appos PBMCs,cells
R446 T508 T505 punct ),cells
R447 T509 T505 prep from,cells
R448 T510 T511 preconj both,CF
R449 T511 T509 pobj CF,from
R450 T512 T511 cc and,CF
R451 T513 T515 amod healthy,subjects
R452 T514 T515 compound control,subjects
R453 T515 T511 conj subjects,CF
R454 T516 T516 ROOT produced,produced
R455 T517 T518 amod large,amounts
R456 T518 T516 dobj amounts,produced
R457 T519 T518 prep of,amounts
R458 T520 T519 pobj IL-1β,of
R459 T521 T516 cc and,produced
R460 T522 T523 advmod strongly,upregulated
R461 T523 T516 conj upregulated,produced
R462 T524 T525 amod caspase-1,activity
R463 T525 T523 dobj activity,upregulated
R464 T526 T523 prep upon,upregulated
R465 T527 T528 amod inflammasome,stimulation
R466 T528 T526 pobj stimulation,upon
R467 T529 T516 punct .,produced
R468 T530 T536 nsubj PBMCs,displayed
R469 T531 T530 prep from,PBMCs
R470 T532 T533 compound CF,patients
R471 T533 T531 pobj patients,from
R472 T534 T533 cc and,patients
R473 T535 T533 conj controls,patients
R474 T536 T536 ROOT displayed,displayed
R475 T537 T538 amod similar,levels
R476 T538 T536 dobj levels,displayed
R477 T539 T538 prep of,levels
R478 T540 T541 amod caspase-1,activation
R479 T541 T539 pobj activation,of
R480 T542 T541 cc and,activation
R481 T543 T544 amod IL-1β,production
R482 T544 T541 conj production,activation
R483 T545 T546 advmod when,stimulated
R484 T546 T536 advcl stimulated,displayed
R485 T547 T546 prep with,stimulated
R337 T399 T400 det the,quality
R338 T400 T390 conj quality,are
R339 T401 T400 prep of,quality
R340 T402 T401 pobj life,of
R341 T403 T402 cc and,life
R342 T404 T400 conj outcome,quality
R343 T405 T400 prep for,quality
R344 T406 T405 pobj patients,for
R345 T407 T406 prep with,patients
R346 T408 T409 compound cystic,fibrosis
R347 T409 T407 pobj fibrosis,with
R348 T410 T411 punct (,CF
R349 T411 T409 appos CF,fibrosis
R350 T412 T400 punct ),quality
R351 T413 T390 punct .,are
R352 T414 T415 compound Interleukin-1,beta
R353 T415 T419 nsubj beta,is
R354 T416 T417 punct (,IL-1β
R355 T417 T415 appos IL-1β,beta
R356 T418 T417 punct ),IL-1β
R357 T419 T419 ROOT is,is
R358 T420 T423 det a,mediator
R359 T421 T423 amod key,mediator
R360 T422 T423 amod inflammatory,mediator
R361 T423 T419 attr mediator,is
R362 T424 T419 punct .,is
R363 T425 T430 nsubj Secretion,involves
R364 T426 T425 prep of,Secretion
R365 T427 T428 advmod biologically,active
R366 T428 T429 amod active,IL-1β
R367 T429 T426 pobj IL-1β,of
R368 T430 T430 ROOT involves,involves
R369 T431 T432 amod inflammasome-mediated,processing
R370 T432 T430 dobj processing,involves
R371 T433 T430 punct .,involves
R372 T434 T436 nsubjpass Little,known
R373 T435 T436 auxpass is,known
R374 T436 T436 ROOT known,known
R375 T437 T436 prep about,known
R376 T438 T439 det the,contribution
R377 T439 T437 pobj contribution,about
R378 T440 T439 prep of,contribution
R379 T441 T440 pobj IL-1β,of
R380 T442 T441 cc and,IL-1β
R381 T443 T444 det the,inflammasomes
R382 T444 T441 conj inflammasomes,IL-1β
R383 T445 T444 prep in,inflammasomes
R384 T446 T448 nmod CF,disease
R385 T447 T448 amod inflammatory,disease
R386 T448 T445 pobj disease,in
R387 T449 T436 punct .,known
R388 T450 T451 det This,study
R389 T451 T452 nsubj study,examines
R390 T452 T452 ROOT examines,examines
R391 T453 T455 amod inflammasome-mediated,production
R392 T454 T455 compound IL-1β,production
R393 T455 T452 dobj production,examines
R394 T456 T455 prep in,production
R395 T457 T461 nmod CF,lines
R396 T458 T461 amod bronchial,lines
R397 T459 T461 amod epithelial,lines
R398 T460 T461 compound cell,lines
R399 T461 T456 pobj lines,in
R400 T462 T461 cc and,lines
R401 T463 T464 amod human,patients
R402 T464 T461 conj patients,lines
R403 T465 T455 prep with,production
R404 T466 T467 compound CF.,Results
R405 T467 T473 nsubjpass Results,found
R406 T468 T471 nmod Bronchial,lines
R407 T469 T471 amod epithelial,lines
R408 T470 T471 compound cell,lines
R409 T471 T473 nsubjpass lines,found
R410 T472 T473 auxpass were,found
R411 T473 T452 conj found,examines
R487 T549 T547 pobj activators,with
R488 T550 T536 punct .,displayed
R489 T551 T553 det This,production
R490 T552 T553 compound IL-1β,production
R491 T553 T554 nsubj production,was
R492 T554 T554 ROOT was,was
R493 T555 T554 acomp dependent,was
R494 T556 T555 prep on,dependent
R495 T557 T558 amod NF-κB,activity
R496 T558 T556 pobj activity,on
R497 T559 T554 cc and,was
R498 T560 T562 aux could,enhanced
R499 T561 T562 auxpass be,enhanced
R500 T562 T554 conj enhanced,was
R501 T563 T562 prep by,enhanced
R502 T564 T563 pcomp priming,by
R503 T565 T564 prep with,priming
R504 T566 T565 pobj LPS,with
R505 T567 T554 punct .,was
R506 T568 T584 advmod Finally,alter
R507 T569 T584 punct ",",alter
R508 T570 T571 compound chemical,inhibition
R509 T571 T584 nsubj inhibition,alter
R510 T572 T571 prep of,inhibition
R511 T573 T574 amod CFTR,activity
R512 T574 T572 pobj activity,of
R513 T575 T574 prep in,activity
R514 T576 T575 pobj control,in
R515 T577 T571 conj PBMCs,inhibition
R516 T578 T577 cc and,PBMCs
R517 T579 T580 nummod THP-1,cells
R518 T580 T577 conj cells,PBMCs
R519 T581 T584 aux did,alter
R520 T582 T584 neg not,alter
R521 T583 T584 advmod significantly,alter
R522 T584 T584 ROOT alter,alter
R523 T585 T588 amod IL-1β,production
R524 T586 T585 cc or,IL-1β
R525 T587 T585 conj IL-8,IL-1β
R526 T588 T584 dobj production,alter
R527 T589 T584 prep in,alter
R528 T590 T589 pobj response,in
R529 T591 T590 prep to,response
R530 T592 T593 compound P.,aeruginosa
R531 T593 T591 pobj aeruginosa,to
R532 T594 T584 punct .,alter
R533 T595 T596 compound Conclusion,Hematopoeitic
R534 T596 T597 compound Hematopoeitic,cells
R535 T597 T598 nsubj cells,appear
R536 T598 T598 ROOT appear,appear
R537 T599 T600 aux to,be
R538 T600 T598 xcomp be,appear
R539 T601 T603 det the,source
R540 T602 T603 amod predominant,source
R541 T603 T600 attr source,be
R542 T604 T603 prep of,source
R543 T605 T607 amod inflammasome-induced,IL-1β
R544 T606 T607 amod pro-inflammatory,IL-1β
R545 T607 T604 pobj IL-1β,of
R546 T608 T607 prep in,IL-1β
R547 T609 T610 compound CF.,PBMCs
R548 T610 T608 pobj PBMCs,in
R549 T611 T598 advcl derived,appear
R550 T612 T611 prep from,derived
R551 T613 T614 compound CF,subjects
R552 T614 T612 pobj subjects,from
R553 T615 T611 dobj display,derived
R554 T616 T618 amod preserved,activation
R555 T617 T618 amod inflammasome,activation
R556 T618 T615 dobj activation,display
R557 T619 T618 cc and,activation
R558 T620 T621 amod IL-1β,secretion
R559 T621 T618 conj secretion,activation
R560 T622 T615 prep in,display
R561 T623 T622 pobj response,in
R562 T624 T623 prep to,response
R563 T625 T628 det the,pathogen
R564 T626 T628 amod major,pathogen
R565 T627 T628 compound CF,pathogen
R566 T628 T624 pobj pathogen,to
R567 T629 T630 compound Pseudomonas,aeruginosa
R568 T630 T628 appos aeruginosa,pathogen
R569 T631 T598 punct .,appear
R486 T548 T549 amod inflammasome,activators
R1612 T1935 T1945 prep Following,produced
R570 T632 T638 advmod However,support
R571 T633 T638 punct ",",support
R572 T634 T635 poss our,data
R573 T635 T638 nsubj data,support
R574 T636 T638 aux do,support
R575 T637 T638 neg not,support
R576 T638 T638 ROOT support,support
R577 T639 T640 det the,hypothesis
R578 T640 T638 dobj hypothesis,support
R579 T641 T642 nsubj that,increased
R580 T642 T640 relcl increased,hypothesis
R581 T643 T644 amod IL-1β,production
R582 T644 T642 dobj production,increased
R583 T645 T644 prep in,production
R584 T646 T647 compound CF,subjects
R585 T647 T645 pobj subjects,in
R586 T648 T638 advcl is,support
R587 T649 T648 acomp due,is
R588 T650 T649 prep to,due
R589 T651 T653 det an,increase
R590 T652 T653 amod intrinsic,increase
R591 T653 T650 pobj increase,to
R592 T654 T653 prep in,increase
R593 T655 T656 amod NF-κB,activity
R594 T656 T654 pobj activity,in
R595 T657 T653 prep through,increase
R596 T658 T657 pobj loss,through
R597 T659 T658 prep of,loss
R598 T660 T661 amod CFTR,function
R599 T661 T659 pobj function,of
R600 T662 T638 punct .,support
R1433 T1659 T1660 amod Cystic,fibrosis
R1434 T1660 T1661 nsubj fibrosis,is
R1435 T1661 T1661 ROOT is,is
R1436 T1662 T1665 det an,disease
R1437 T1663 T1665 amod autosomal,disease
R1438 T1664 T1665 amod recessive,disease
R1439 T1665 T1661 attr disease,is
R1440 T1666 T1665 acl caused,disease
R1441 T1667 T1666 agent by,caused
R1442 T1668 T1667 pobj mutations,by
R1443 T1669 T1668 prep in,mutations
R1444 T1670 T1675 det the,regulator
R1445 T1671 T1675 amod cystic,regulator
R1446 T1672 T1675 nmod fibrosis,regulator
R1447 T1673 T1675 nmod transmembrane,regulator
R1448 T1674 T1675 compound conductance,regulator
R1449 T1675 T1669 pobj regulator,in
R1450 T1676 T1675 punct (,regulator
R1451 T1677 T1675 appos CFTR,regulator
R1452 T1678 T1675 punct ),regulator
R1453 T1679 T1675 punct ",",regulator
R1454 T1680 T1681 nsubj which,functions
R1455 T1681 T1668 relcl functions,mutations
R1456 T1682 T1681 prep as,functions
R1457 T1683 T1686 det a,channel
R1458 T1684 T1686 compound chloride,channel
R1459 T1685 T1686 compound ion,channel
R1460 T1686 T1682 pobj channel,as
R1461 T1687 T1661 punct .,is
R1462 T1688 T1689 nsubj CF,remains
R1463 T1689 T1689 ROOT remains,remains
R1464 T1690 T1689 attr one,remains
R1465 T1691 T1690 prep of,one
R1466 T1692 T1697 det the,diseases
R1467 T1693 T1694 advmod most,common
R1468 T1694 T1697 amod common,diseases
R1469 T1695 T1697 amod lethal,diseases
R1470 T1696 T1697 amod genetic,diseases
R1471 T1697 T1691 pobj diseases,of
R1472 T1698 T1697 prep in,diseases
R1473 T1699 T1698 pobj populations,in
R1474 T1700 T1699 prep of,populations
R1475 T1701 T1702 amod European,descent
R1476 T1702 T1700 pobj descent,of
R1477 T1703 T1690 prep with,one
R1478 T1704 T1707 det the,lifespan
R1479 T1705 T1707 amod current,lifespan
R1480 T1706 T1707 amod average,lifespan
R1481 T1707 T1703 pobj lifespan,with
R1482 T1708 T1707 prep of,lifespan
R1483 T1709 T1710 compound CF,patients
R1484 T1710 T1708 pobj patients,of
R1485 T1711 T1712 advmod approximately,40
R1486 T1712 T1713 nummod 40,years
R1487 T1713 T1707 appos years,lifespan
R1488 T1714 T1713 prep of,years
R1489 T1715 T1714 pobj age,of
R1490 T1716 T1718 nmod [,]
R1491 T1717 T1718 nummod 1,]
R1492 T1718 T1713 appos ],years
R1493 T1719 T1718 punct ",",]
R1494 T1720 T1722 nmod [,]
R1495 T1721 T1722 nummod 2,]
R1496 T1722 T1718 appos ],]
R1497 T1723 T1689 punct .,remains
R1498 T1724 T1727 amod Recurrent,exacerbation
R1499 T1725 T1727 amod inflammatory,exacerbation
R1500 T1726 T1727 amod pulmonary,exacerbation
R1501 T1727 T1728 nsubj exacerbation,is
R1502 T1728 T1728 ROOT is,is
R1503 T1729 T1731 det the,cause
R1504 T1730 T1731 amod primary,cause
R1505 T1731 T1728 attr cause,is
R1506 T1732 T1731 prep of,cause
R1507 T1733 T1734 compound lung,disease
R1508 T1734 T1735 compound disease,progression
R1509 T1735 T1732 pobj progression,of
R1510 T1736 T1731 cc and,cause
R1511 T1737 T1744 advmod ultimately,is
R1512 T1738 T1744 punct ",",is
R1513 T1739 T1744 nsubj death,is
R1514 T1740 T1739 prep in,death
R1515 T1741 T1743 compound CF.,inflammation
R1516 T1742 T1743 compound Controlled,inflammation
R1517 T1743 T1740 pobj inflammation,in
R1518 T1744 T1728 conj is,is
R1519 T1745 T1744 acomp important,is
R1520 T1746 T1745 prep for,important
R1521 T1747 T1746 pcomp fighting,for
R1522 T1748 T1747 dobj infection,fighting
R1523 T1749 T1744 punct ",",is
R1524 T1750 T1744 cc but,is
R1525 T1751 T1755 prep in,becomes
R1526 T1752 T1751 pobj excess,in
R1527 T1753 T1755 punct ",",becomes
R1528 T1754 T1755 nsubj it,becomes
R1529 T1755 T1744 conj becomes,is
R1530 T1756 T1755 acomp destructive,becomes
R1531 T1757 T1758 aux to,host
R1532 T1758 T1755 xcomp host,becomes
R1533 T1759 T1758 dobj cells,host
R1534 T1760 T1758 cc and,host
R1535 T1761 T1758 prep to,host
R1536 T1762 T1763 det the,architecture
R1537 T1763 T1761 pobj architecture,to
R1538 T1764 T1763 prep of,architecture
R1539 T1765 T1766 det the,lungs
R1540 T1766 T1764 pobj lungs,of
R1541 T1767 T1769 nmod [,]
R1542 T1768 T1769 nummod 2,]
R1543 T1769 T1773 nmod ],]
R1544 T1770 T1769 punct ",",]
R1545 T1771 T1773 nmod [,]
R1546 T1772 T1773 nummod 3,]
R1547 T1773 T1755 npadvmod ],becomes
R1548 T1774 T1755 punct .,becomes
R1549 T1775 T1783 prep In,shown
R1550 T1776 T1775 pobj CF,In
R1551 T1777 T1783 punct ",",shown
R1552 T1778 T1780 amod airway,cells
R1553 T1779 T1780 amod epithelial,cells
R1554 T1780 T1783 nsubjpass cells,shown
R1555 T1781 T1783 aux have,shown
R1556 T1782 T1783 auxpass been,shown
R1557 T1783 T1783 ROOT shown,shown
R1558 T1784 T1785 aux to,produce
R1559 T1785 T1783 xcomp produce,shown
R1613 T1936 T1935 pobj initiation,Following
R1614 T1937 T1936 prep of,initiation
R1615 T1938 T1939 det the,NF-κB
R1616 T1939 T1937 pobj NF-κB,of
R1617 T1940 T1936 acl signaling,initiation
R1560 T1786 T1790 det an,response
R1561 T1787 T1790 amod exaggerated,response
R1562 T1788 T1790 amod pro-inflammatory,response
R1563 T1789 T1790 compound cytokine,response
R1564 T1790 T1785 dobj response,produce
R1565 T1791 T1792 aux to,stimulation
R1566 T1792 T1790 relcl stimulation,response
R1567 T1793 T1795 nmod [,]
R1568 T1794 T1795 nummod 4,]
R1569 T1795 T1792 dobj ],stimulation
R1570 T1796 T1795 punct ",",]
R1571 T1797 T1795 appos [,]
R1572 T1798 T1799 nummod 5,]
R1573 T1799 T1795 conj ],]
R1574 T1800 T1783 punct .,shown
R1575 T1801 T1802 nsubj It,is
R1576 T1802 T1802 ROOT is,is
R1577 T1803 T1802 acomp unclear,is
R1578 T1804 T1809 mark whether,is
R1579 T1805 T1808 det this,response
R1580 T1806 T1808 amod heightened,response
R1581 T1807 T1808 amod inflammatory,response
R1582 T1808 T1809 nsubj response,is
R1583 T1809 T1802 ccomp is,is
R1584 T1810 T1809 acomp intrinsic,is
R1585 T1811 T1810 prep to,intrinsic
R1586 T1812 T1811 pobj cells,to
R1587 T1813 T1812 acl lacking,cells
R1588 T1814 T1813 dobj CFTR,lacking
R1589 T1815 T1809 cc or,is
R1590 T1816 T1818 mark whether,is
R1591 T1817 T1818 nsubj it,is
R1592 T1818 T1809 conj is,is
R1593 T1819 T1820 det a,result
R1594 T1820 T1818 attr result,is
R1595 T1821 T1820 prep of,result
R1596 T1822 T1825 amod chronic,[
R1597 T1823 T1824 amod polymicrobial,infection
R1598 T1824 T1825 compound infection,[
R1599 T1825 T1827 nmod [,]
R1600 T1826 T1827 nummod 6,]
R1601 T1827 T1821 pobj ],of
R1602 T1828 T1827 punct ",",]
R1603 T1829 T1831 nmod [,]
R1604 T1830 T1831 nummod 7,]
R1605 T1831 T1827 appos ],]
R1606 T1832 T1802 punct .,is
R1607 T1833 T1844 advmod Regardless,is
R1608 T1834 T1833 prep of,Regardless
R1609 T1835 T1836 det this,controversy
R1610 T1836 T1834 pobj controversy,of
R1611 T1837 T1833 punct ",",Regardless
R1687 T1968 T1968 ROOT termed,termed
R1688 T1969 T1968 oprd inflammasomes,termed
R1689 T1880 T1881 nsubj effects,are
R1690 T1881 T1881 ROOT are,are
R1691 T1970 T1959 punct .,converted
R1692 T1882 T1881 acomp diverse,are
R1693 T1883 T1882 cc and,diverse
R1694 T1971 T1972 nsubj Inflammasomes,assemble
R1695 T1884 T1885 advmod potentially,important
R1696 T1885 T1882 conj important,diverse
R1697 T1972 T1972 ROOT assemble,assemble
R1698 T1886 T1885 prep to,important
R1699 T1887 T1888 det the,pathogenesis
R1700 T1973 T1972 prep in,assemble
R1701 T1888 T1886 pobj pathogenesis,to
R1702 T1889 T1888 prep of,pathogenesis
R1703 T1890 T1891 compound lung,exacerbations
R1704 T1891 T1889 pobj exacerbations,of
R1705 T1974 T1973 pobj response,in
R1706 T1892 T1891 prep in,exacerbations
R1707 T1893 T1892 pobj CF,in
R1708 T1975 T1974 prep to,response
R1709 T1894 T1891 punct ",",exacerbations
R1710 T1895 T1888 prep including,pathogenesis
R1711 T1976 T1979 amod certain,signals
R1712 T1896 T1897 det the,generation
R1713 T1897 T1895 pobj generation,including
R1714 T1898 T1897 prep of,generation
R1715 T1977 T1978 amod cellular,danger
R1716 T1899 T1898 pobj fever,of
R1717 T1900 T1888 punct ",",pathogenesis
R1718 T1978 T1979 compound danger,signals
R1719 T1901 T1902 det the,recruitment
R1720 T1902 T1888 appos recruitment,pathogenesis
R1721 T1979 T1975 pobj signals,to
R1722 T1903 T1902 prep of,recruitment
R1723 T1904 T1906 amod inflammatory,cells
R1724 T1905 T1906 compound effector,cells
R1725 T1980 T1972 cc and,assemble
R1726 T1906 T1903 pobj cells,of
R1727 T1907 T1888 punct ",",pathogenesis
R1728 T1981 T1972 conj mediate,assemble
R1729 T1908 T1909 det the,induction
R1730 T1909 T1927 nsubj induction,[
R1731 T1910 T1909 prep of,induction
R1732 T1911 T1913 amod other,cytokines
R1733 T1912 T1913 amod pro-inflammatory,cytokines
R1734 T1982 T1983 det the,auto-activation
R1735 T1913 T1910 pobj cytokines,of
R1736 T1914 T1915 amod such,as
R1737 T1983 T1981 dobj auto-activation,mediate
R1738 T1915 T1913 prep as,cytokines
R1739 T1916 T1915 pobj IL-6,as
R1740 T1917 T1916 cc and,IL-6
R1741 T1984 T1983 prep of,auto-activation
R1742 T1918 T1916 conj IL-8,IL-6
R1743 T1919 T1918 punct ",",IL-8
R1744 T1985 T1986 amod caspase-1,[
R1745 T1920 T1918 cc and,IL-8
R1746 T1921 T1922 det the,shaping
R1747 T1922 T1918 conj shaping,IL-8
R1748 T1923 T1922 prep of,shaping
R1749 T1986 T1988 nmod [,]
R1750 T1924 T1926 compound T,responses
R1751 T1925 T1926 compound cell,responses
R1752 T1987 T1986 nummod 9,[
R1753 T1926 T1923 pobj responses,of
R1754 T1927 T1881 conj [,are
R1755 T1928 T1929 nummod 9,]
R1756 T1929 T1927 dobj ],[
R1757 T1930 T1929 punct ",",]
R1758 T1931 T1929 appos [,]
R1759 T1988 T1984 pobj ],of
R1760 T1932 T1933 nummod 10,]
R1761 T1933 T1929 appos ],]
R1762 T1934 T1927 punct .,[
R1763 T1989 T1988 punct ",",]
R1764 T1990 T1992 compound [,]
R1765 T1991 T1992 compound 11,]
R1766 T1992 T1983 appos ],auto-activation
R1767 T1993 T1992 punct ",",]
R1768 T1994 T1995 nsubj which,cleaves
R1769 T2032 T2029 conj [,]
R1770 T2033 T2034 nummod 15,]
R1771 T1995 T1992 relcl cleaves,]
R1772 T2034 T2032 conj ],[
R1773 T2035 T2034 punct ",",]
R1774 T2036 T2034 conj [,]
R1775 T1996 T1995 dobj pro-IL-1β,cleaves
R1776 T2037 T2038 nummod 16,]
R1777 T2038 T2034 conj ],]
R1778 T1997 T1996 cc and,pro-IL-1β
R1779 T2039 T2038 punct ",",]
R1780 T2040 T2038 cc and,]
R1781 T1998 T1996 conj pro-IL-18,pro-IL-1β
R1782 T2041 T2038 conj AIM2,]
R1783 T2042 T2043 compound inflammasomes,[
R1784 T2043 T2020 conj [,]
R1785 T1999 T1995 prep into,cleaves
R1786 T2044 T2045 nummod 17,]
R1787 T2045 T2043 conj ],[
R1788 T2046 T2045 punct ",",]
R1789 T2000 T2003 poss their,forms
R1790 T2047 T2049 nmod [,]
R1791 T2048 T2049 nummod 18,]
R1792 T2049 T2045 conj ],]
R1618 T1941 T1940 dobj cascade,signaling
R1619 T1942 T1945 punct ",",produced
R1620 T1943 T1945 nsubjpass IL-1β,produced
R1621 T1944 T1945 auxpass is,produced
R1622 T1945 T1945 ROOT produced,produced
R1623 T1946 T1945 prep in,produced
R1624 T1947 T1948 det the,cytosol
R1625 T1948 T1946 pobj cytosol,in
R1626 T1949 T1945 prep as,produced
R1627 T1950 T1954 det a,pro-IL-1β
R1628 T1838 T1844 csubj identifying,is
R1629 T1951 T1952 advmod biologically,inactive
R1630 T1839 T1838 cc and,identifying
R1631 T1952 T1954 amod inactive,pro-IL-1β
R1632 T1840 T1838 conj targeting,identifying
R1633 T1841 T1843 amod relevant,mediators
R1634 T1953 T1954 amod full-length,pro-IL-1β
R1635 T1842 T1843 amod inflammatory,mediators
R1793 T2001 T2002 advmod biologically,active
R1794 T2002 T2003 amod active,forms
R1795 T2050 T2049 punct ",",]
R1796 T2003 T1999 pobj forms,into
R1797 T2004 T2003 prep for,forms
R1798 T2051 T2052 nsubj which,respond
R1799 T2005 T2004 pobj secretion,for
R1800 T2052 T2020 relcl respond,]
R1801 T2053 T2052 prep to,respond
R1802 T2006 T1972 punct .,assemble
R1803 T2054 T2055 det a,variety
R1804 T2055 T2053 pobj variety,to
R1805 T2056 T2055 prep of,variety
R1806 T2057 T2059 amod different,signatures
R1807 T2007 T2009 nummod Four,inflammasomes
R1808 T2058 T2059 amod microbial,signatures
R1809 T2059 T2056 pobj signatures,of
R1810 T2060 T2059 cc and,signatures
R1811 T2008 T2009 amod distinct,inflammasomes
R1812 T2061 T2062 nsubj danger,signals
R1813 T2009 T2012 nsubjpass inflammasomes,recognized
R1814 T2010 T2012 aux have,recognized
R1815 T2062 T2059 conj signals,signatures
R1816 T2011 T2012 auxpass been,recognized
R1817 T2012 T2012 ROOT recognized,recognized
R1818 T2063 T2065 nmod [,]
R1819 T2064 T2065 nummod 11,]
R1820 T2013 T2012 punct .,recognized
R1821 T2065 T2055 appos ],variety
R1822 T2066 T2015 punct .,are
R1823 T2014 T2015 nsubj These,are
R1824 T2067 T2068 compound P.,aeruginosa
R1825 T2068 T2083 nsubj aeruginosa,activates
R1826 T2015 T2015 ROOT are,are
R1827 T2069 T2068 punct ",",aeruginosa
R1828 T2070 T2068 appos one,aeruginosa
R1829 T2071 T2070 prep of,one
R1830 T2016 T2020 det the,]
R1831 T2072 T2078 det the,pathogens
R1832 T2073 T2074 advmod most,common
R1833 T2017 T2018 compound NLRP1,[
R1834 T2074 T2078 amod common,pathogens
R1835 T2075 T2074 cc and,common
R1836 T2076 T2077 advmod clinically,relevant
R1837 T2018 T2020 nmod [,]
R1838 T2077 T2074 conj relevant,common
R1839 T2078 T2071 pobj pathogens,of
R1840 T2019 T2020 nummod 12,]
R1841 T2079 T2078 prep among,pathogens
R1842 T2080 T2081 compound CF,patients
R1843 T2081 T2079 pobj patients,among
R1844 T2020 T2015 attr ],are
R1845 T2082 T2068 punct ",",aeruginosa
R1846 T2083 T2083 ROOT activates,activates
R1847 T2021 T2020 punct ",",]
R1848 T2084 T2089 det the,]
R1849 T2085 T2087 nmod NLRC4,[
R1850 T2086 T2087 compound inflammasome,[
R1851 T2022 T2023 compound NLRP3,[
R1852 T2087 T2089 nmod [,]
R1853 T2088 T2089 nummod 19,]
R1854 T2089 T2083 dobj ],activates
R1855 T2090 T2089 punct ",",]
R1856 T2091 T2089 conj [,]
R1857 T2023 T2020 appos [,]
R1858 T2024 T2025 nummod 13,]
R1859 T2092 T2093 nummod 20,]
R1860 T2093 T2089 conj ],]
R1861 T2025 T2020 conj ],]
R1862 T2094 T2083 punct .,activates
R1863 T2095 T2099 nsubj Infection,triggers
R1864 T2026 T2025 punct ",",]
R1865 T2096 T2095 prep with,Infection
R1866 T2097 T2098 compound P.,aeruginosa
R1867 T2098 T2096 pobj aeruginosa,with
R1868 T2027 T2025 conj [,]
R1869 T2099 T2099 ROOT triggers,triggers
R1870 T2100 T2101 det an,increase
R1871 T2028 T2029 nummod 14,]
R1872 T2101 T2099 dobj increase,triggers
R1873 T2102 T2101 prep in,increase
R1874 T2029 T2025 conj ],]
R1875 T2103 T2102 pobj levels,in
R1876 T2104 T2103 prep of,levels
R1877 T2105 T2104 pobj IL-1β,of
R1878 T2030 T2029 punct ",",]
R1879 T2106 T2105 punct ",",IL-1β
R1880 T2107 T2105 conj IL-6,IL-1β
R1881 T2031 T2032 compound NLRC4,[
R1882 T2108 T2107 punct ",",IL-6
R1883 T2109 T2107 cc and,IL-6
R1884 T2110 T2107 conj IL-8,IL-6
R1885 T2129 T2128 prep on,depend
R1886 T2111 T2107 prep in,IL-6
R1887 T2112 T2114 nmod bronchoalveolar,fluid
R1888 T2113 T2114 compound lavage,fluid
R1889 T2114 T2111 pobj fluid,in
R1890 T2115 T2116 punct (,BALF
R1891 T2116 T2114 appos BALF,fluid
R1892 T2130 T2131 amod NF-κB,signaling
R1893 T2117 T2116 punct ),BALF
R1894 T2118 T2101 prep from,increase
R1895 T2131 T2129 pcomp signaling,on
R1896 T2119 T2118 pobj patients,from
R1897 T2120 T2119 prep with,patients
R1898 T2121 T2122 compound CF,[
R1899 T2132 T2131 punct ",",signaling
R1900 T2122 T2120 pobj [,with
R1901 T2123 T2124 nummod 21,]
R1902 T2124 T2122 appos ],[
R1903 T2133 T2135 advmod where,is
R1904 T2125 T2099 punct .,triggers
R1905 T2126 T2127 amod Inflammasome,responses
R1906 T2127 T2128 nsubj responses,depend
R1907 T2128 T2128 ROOT depend,depend
R1908 T2134 T2135 nsubj NF-κB,is
R1909 T2135 T2129 pcomp is,on
R1910 T2136 T2135 acomp important,is
R1911 T2226 T2234 nsubj Polymorphisms,associated
R1912 T2137 T2135 prep in,is
R1913 T2227 T2226 prep in,Polymorphisms
R1914 T2228 T2230 det the,gene
R1915 T2229 T2230 compound IL1B,gene
R1916 T2138 T2140 preconj both,upregulation
R1917 T2230 T2227 pobj gene,in
R1918 T2231 T2234 aux have,associated
R1919 T2139 T2140 det the,upregulation
R1920 T2232 T2234 advmod also,associated
R1921 T2233 T2234 auxpass been,associated
R1922 T2234 T2234 ROOT associated,associated
R1923 T2140 T2137 pobj upregulation,in
R1924 T2235 T2234 prep with,associated
R1925 T2236 T2237 amod varying,degrees
R1926 T2237 T2235 pobj degrees,with
R1927 T2141 T2140 prep of,upregulation
R1928 T2142 T2144 amod specific,components
R1929 T2238 T2237 prep of,degrees
R1930 T2239 T2240 compound disease,severity
R1931 T2143 T2144 amod inflammasome,components
R1932 T2240 T2238 pobj severity,of
R1933 T2241 T2240 prep in,severity
R1934 T2242 T2243 compound CF,patients
R1935 T2144 T2141 pobj components,of
R1936 T2243 T2241 pobj patients,in
R1937 T2244 T2243 appos [,patients
R1938 T2245 T2246 nummod 29,]
R1939 T2145 T2147 nmod [,]
R1940 T2246 T2244 appos ],[
R1941 T2247 T2234 punct .,associated
R1942 T2248 T2249 compound Murine,models
R1943 T2146 T2147 nummod 22,]
R1944 T2249 T2254 nsubj models,exhibited
R1945 T2250 T2249 prep of,models
R1946 T2251 T2252 amod CFTR,dysfunction
R1947 T2147 T2140 appos ],upregulation
R1948 T2252 T2250 pobj dysfunction,of
R1949 T2253 T2254 aux have,exhibited
R1950 T2254 T2254 ROOT exhibited,exhibited
R1636 T1843 T1840 dobj mediators,targeting
R1637 T1844 T1844 ROOT is,is
R1638 T1954 T1949 pobj pro-IL-1β,as
R1639 T1845 T1847 det a,step
R1640 T1846 T1847 amod critical,step
R1641 T1955 T1945 punct .,produced
R1642 T1956 T1959 nsubjpass Pro-IL-1β,converted
R1643 T1847 T1844 attr step,is
R1644 T1957 T1959 auxpass is,converted
R1645 T1848 T1847 prep in,step
R1646 T1958 T1959 advmod subsequently,converted
R1647 T1849 T1848 pcomp developing,in
R1648 T1850 T1851 advmod more,specific
R1649 T1851 T1853 amod specific,approaches
R1650 T1852 T1853 amod therapeutic,approaches
R1651 T1959 T1959 ROOT converted,converted
R1652 T1853 T1849 dobj approaches,developing
R1653 T1854 T1855 aux to,control
R1654 T1855 T1853 relcl control,approaches
R1655 T1856 T1855 dobj inflammation,control
R1656 T1857 T1855 cc and,control
R1657 T1858 T1855 conj improve,control
R1658 T1859 T1860 compound health,outcomes
R1659 T1960 T1959 prep into,converted
R1660 T1860 T1858 dobj outcomes,improve
R1661 T1861 T1860 prep in,outcomes
R1662 T1862 T1863 compound CF,[
R1663 T1961 T1963 poss its,form
R1664 T1863 T1861 pobj [,in
R1665 T1864 T1865 nummod 8,]
R1951 T2148 T2147 punct ",",]
R1952 T2255 T2256 amod significant,increases
R1953 T2256 T2254 dobj increases,exhibited
R1954 T2257 T2256 prep in,increases
R1955 T2258 T2259 amod IL-1β,expression
R1956 T2259 T2257 pobj expression,in
R1957 T2260 T2259 cc or,expression
R1958 T2149 T2151 nmod [,]
R1959 T2261 T2259 conj secretion,expression
R1960 T2262 T2259 prep in,expression
R1961 T2263 T2262 pobj macrophages,in
R1962 T2264 T2266 nmod [,]
R1963 T2150 T2151 nummod 23,]
R1964 T2265 T2266 nummod 30,]
R1965 T2266 T2256 conj ],increases
R1966 T2151 T2147 appos ],]
R1967 T2152 T2151 punct ",",]
R1968 T2267 T2266 punct ",",]
R1969 T2268 T2270 nmod [,]
R1970 T2153 T2155 advmod as,as
R1971 T2269 T2270 nummod 31,]
R1972 T2270 T2266 conj ],]
R1973 T2154 T2155 advmod well,as
R1974 T2271 T2270 punct ",",]
R1975 T2272 T2254 cc and,exhibited
R1976 T2273 T2254 conj support,exhibited
R1977 T2155 T2151 cc as,]
R1978 T2274 T2275 det the,hypothesis
R1979 T2275 T2273 dobj hypothesis,support
R1980 T2156 T2157 amod IL-1β,expression
R1981 T2276 T2281 mark that,increases
R1982 T2277 T2278 det the,loss
R1983 T2278 T2281 nsubj loss,increases
R1984 T2157 T2160 nmod expression,]
R1985 T2279 T2278 prep of,loss
R1986 T2280 T2281 compound CFTR,increases
R1987 T2281 T2275 acl increases,hypothesis
R1988 T2158 T2160 nmod [,]
R1989 T2282 T2283 amod NF-κB,activation
R1990 T2283 T2281 dobj activation,increases
R1991 T2284 T2283 prep under,activation
R1992 T2159 T2160 nummod 24,]
R1993 T2285 T2284 pobj basal,under
R1994 T2160 T2151 conj ],]
R1995 T2286 T2285 cc and,basal
R1996 T2287 T2285 conj stimulatory,basal
R1997 T2161 T2160 punct ",",]
R1998 T2288 T2289 nsubj conditions,[
R1999 T2289 T2291 nmod [,]
R2000 T2290 T2291 nummod 4,]
R2001 T2162 T2164 compound [,]
R2002 T2291 T2291 ROOT ],]
R2003 T2292 T2291 punct ",",]
R2004 T2163 T2164 nummod 25,]
R2005 T2293 T2295 nmod [,]
R2006 T2294 T2295 nummod 5,]
R2007 T2295 T2291 conj ],]
R2008 T2296 T2295 punct ",",]
R2009 T2297 T2295 conj [,]
R2010 T2298 T2299 nummod 32,]
R2011 T2164 T2160 appos ],]
R2012 T2299 T2295 conj ],]
R2013 T2300 T2299 punct ",",]
R2014 T2301 T2299 conj [,]
R2015 T2165 T2128 punct .,depend
R2016 T2302 T2303 nummod 33,]
R2017 T2303 T2299 conj ],]
R2018 T2304 T2254 punct .,exhibited
R2019 T2166 T2167 amod Previous,studies
R2020 T2305 T2319 advmod Finally,increases
R2021 T2306 T2319 punct ",",increases
R2022 T2307 T2319 nsubj replacement,increases
R2023 T2167 T2168 nsubj studies,support
R2024 T2308 T2307 prep of,replacement
R2025 T2309 T2310 compound chloride,ions
R2026 T2168 T2168 ROOT support,support
R2027 T2310 T2308 pobj ions,of
R2028 T2311 T2307 prep with,replacement
R2029 T2312 T2311 pobj glutamate,with
R2030 T2313 T2312 cc or,glutamate
R2031 T2169 T2170 det a,role
R2032 T2314 T2312 conj gluconate,glutamate
R2033 T2315 T2307 prep in,replacement
R2034 T2170 T2168 dobj role,support
R2035 T2316 T2317 compound cell,culture
R2036 T2317 T2318 compound culture,media
R2037 T2318 T2315 pobj media,in
R2038 T2171 T2170 prep for,role
R2039 T2319 T2319 ROOT increases,increases
R2040 T2320 T2319 dobj secretion,increases
R2041 T2321 T2320 prep of,secretion
R2042 T2172 T2171 pobj IL-1β,for
R2043 T2322 T2321 pobj IL-1β,of
R2044 T2173 T2170 prep in,role
R2045 T2174 T2175 det the,pathogenesis
R2046 T2175 T2173 pobj pathogenesis,in
R2047 T2323 T2319 prep in,increases
R2048 T2176 T2175 prep of,pathogenesis
R2049 T2177 T2180 nmod CF,disease
R2050 T2324 T2323 pobj response,in
R2051 T2178 T2180 amod inflammatory,disease
R2052 T2179 T2180 compound lung,disease
R2053 T2325 T2324 prep to,response
R2054 T2326 T2327 nummod NLRP3,stimulation
R2055 T2327 T2325 pobj stimulation,to
R2056 T2328 T2319 prep by,increases
R2057 T2329 T2330 amod adenosine,triphosphate
R2058 T2330 T2328 pobj triphosphate,by
R2059 T2180 T2176 pobj disease,of
R2060 T2331 T2332 punct (,ATP
R2061 T2332 T2330 appos ATP,triphosphate
R2062 T2333 T2332 punct ),ATP
R2063 T2334 T2336 nmod [,]
R2064 T2181 T2168 punct .,support
R2065 T2335 T2336 nummod 34,]
R2066 T2336 T2330 conj ],triphosphate
R2067 T2337 T2319 punct ",",increases
R2068 T2182 T2186 nsubjpass Levels,increased
R2069 T2338 T2319 advcl implying,increases
R2070 T2339 T2341 det an,role
R2071 T2340 T2341 amod inhibitory,role
R2072 T2183 T2182 prep of,Levels
R2073 T2341 T2338 dobj role,implying
R2074 T2342 T2341 prep for,role
R2075 T2343 T2344 amod extracellular,chloride
R2076 T2184 T2183 pobj IL-1β,of
R2077 T2344 T2342 pobj chloride,for
R2078 T2345 T2341 prep in,role
R2079 T2346 T2347 nummod NLRP3,activation
R2080 T2185 T2186 auxpass are,increased
R2081 T2347 T2345 pobj activation,in
R2082 T2348 T2319 punct .,increases
R2083 T2186 T2186 ROOT increased,increased
R2084 T2349 T2354 advcl Taken,implicate
R2085 T2350 T2349 advmod together,Taken
R2086 T2351 T2354 punct ",",implicate
R2087 T2187 T2186 prep in,increased
R2088 T2352 T2353 det these,data
R2089 T2353 T2354 nsubj data,implicate
R2090 T2354 T2354 ROOT implicate,implicate
R2091 T2188 T2187 pobj BALF,in
R2092 T2355 T2356 det the,involvement
R2093 T2189 T2186 prep from,increased
R2094 T2356 T2354 dobj involvement,implicate
R2095 T2357 T2356 prep of,involvement
R2096 T2190 T2191 compound CF,patients
R2097 T2358 T2357 pobj IL-1β,of
R2098 T2359 T2354 cc and,implicate
R2099 T2360 T2363 advmod consequently,inflammasomes
R2100 T2191 T2189 pobj patients,from
R2101 T2361 T2363 punct ",",inflammasomes
R2102 T2362 T2363 det the,inflammasomes
R2103 T2363 T2354 conj inflammasomes,implicate
R2104 T2192 T2186 prep with,increased
R2105 T2364 T2363 punct ",",inflammasomes
R2106 T2365 T2363 prep in,inflammasomes
R2107 T2193 T2192 pobj infection,with
R2108 T2366 T2368 nmod CF,disease
R1666 T1865 T1863 appos ],[
R1667 T1962 T1963 amod active,form
R1668 T1866 T1844 punct .,is
R1669 T1963 T1960 pobj form,into
R1670 T1867 T1868 compound Interleukin-1,beta
R1671 T1868 T1872 nsubj beta,is
R1672 T1869 T1868 punct (,beta
R1673 T1870 T1868 appos IL-1β,beta
R1674 T1964 T1959 agent by,converted
R1675 T1871 T1868 punct ),beta
R1676 T1872 T1872 ROOT is,is
R1677 T1965 T1967 amod cytosolic,complexes
R1678 T1873 T1876 det a,mediator
R1679 T1874 T1876 amod major,mediator
R1680 T1875 T1876 amod inflammatory,mediator
R1681 T1966 T1967 compound protein,complexes
R1682 T1876 T1872 attr mediator,is
R1683 T1877 T1872 punct .,is
R1684 T1967 T1964 pobj complexes,by
R1685 T1878 T1880 poss Its,effects
R1686 T1879 T1880 amod physiological,effects
R2587 T2963 T2966 nummod S9,cells
R2588 T2964 T2963 cc and,S9
R2589 T2965 T2963 conj IB3-1,S9
R2590 T2966 T2962 pobj cells,in
R2591 T2967 T2962 punct ",",in
R2592 T2968 T2962 conj by,in
R2593 T2969 T2970 nummod 24,hours
R2594 T2970 T2968 pobj hours,by
R2595 T2971 T2930 punct .,increased
R2596 T2972 T2978 prep In,were
R2109 T2367 T2368 amod inflammatory,disease
R2110 T2194 T2196 nmod [,]
R2111 T2368 T2365 pobj disease,in
R2112 T2369 T2354 punct .,implicate
R2113 T2195 T2196 nummod 21,]
R2114 T2196 T2215 nsubjpass ],associated
R2115 T2197 T2196 punct ",",]
R2116 T2198 T2196 appos [,]
R2117 T2199 T2200 nummod 26,]
R2118 T2200 T2196 conj ],]
R2119 T2201 T2200 punct ",",]
R2120 T2202 T2204 nmod [,]
R2121 T2203 T2204 nummod 27,]
R2122 T2204 T2200 conj ],]
R2123 T2205 T2204 punct ",",]
R2124 T2206 T2208 compound [,]
R2125 T2207 T2208 compound 28,]
R2126 T2208 T2204 conj ],]
R2127 T2209 T2208 cc and,]
R2128 T2210 T2211 det this,increase
R2129 T2211 T2208 conj increase,]
R2130 T2212 T2215 aux has,associated
R2131 T2213 T2215 auxpass been,associated
R2132 T2214 T2215 advmod temporally,associated
R2133 T2215 T2186 conj associated,increased
R2134 T2216 T2215 prep with,associated
R2135 T2217 T2219 det a,response
R2136 T2218 T2219 amod clinical,response
R2137 T2219 T2216 pobj response,with
R2138 T2220 T2219 prep to,response
R2139 T2221 T2220 pobj treatment,to
R2140 T2222 T2221 dobj [,treatment
R2141 T2223 T2224 nummod 21,]
R2142 T2224 T2222 appos ],[
R2143 T2225 T2215 punct .,associated
R2430 T2806 T2808 nmod Airway,cells
R2431 T2807 T2808 amod epithelial,cells
R2432 T2808 T2811 nsubj cells,produce
R2433 T2809 T2811 aux do,produce
R2434 T2810 T2811 neg not,produce
R2435 T2811 T2811 ROOT produce,produce
R2436 T2812 T2813 amod significant,amounts
R2437 T2813 T2811 dobj amounts,produce
R2438 T2814 T2813 prep of,amounts
R2439 T2815 T2814 pobj IL-1β,of
R2440 T2816 T2811 prep in,produce
R2441 T2817 T2816 pobj response,in
R2442 T2818 T2817 prep to,response
R2443 T2819 T2820 amod inflammasome,stimulation
R2444 T2820 T2818 pobj stimulation,to
R2445 T2821 T2822 det The,inflammasomes
R2446 T2822 T2827 nsubj inflammasomes,examined
R2447 T2823 T2822 cc and,inflammasomes
R2448 T2824 T2826 poss their,activators
R2449 T2825 T2826 amod respective,activators
R2450 T2826 T2822 conj activators,inflammasomes
R2451 T2827 T2811 conj examined,produce
R2452 T2828 T2827 prep in,examined
R2453 T2829 T2830 det this,study
R2454 T2830 T2828 pobj study,in
R2455 T2831 T2832 auxpass are,listed
R2456 T2832 T2827 conj listed,examined
R2457 T2833 T2832 prep in,listed
R2458 T2834 T2833 pobj Table,in
R2459 T2835 T2834 nummod 1,Table
R2460 T2836 T2827 punct .,examined
R2461 T2837 T2839 nsubjpass Cells,stimulated
R2462 T2838 T2839 auxpass were,stimulated
R2463 T2839 T2839 ROOT stimulated,stimulated
R2464 T2840 T2839 prep in,stimulated
R2465 T2841 T2840 pobj accordance,in
R2466 T2842 T2841 prep with,accordance
R2467 T2843 T2844 det the,schedule
R2468 T2844 T2842 pobj schedule,with
R2469 T2845 T2844 prep in,schedule
R2470 T2846 T2845 pobj Figure,in
R2471 T2847 T2846 nummod 1,Figure
R2472 T2848 T2839 punct .,stimulated
R2473 T2849 T2857 prep In,shown
R2474 T2850 T2849 pobj CF,In
R2475 T2851 T2857 punct ",",shown
R2476 T2852 T2854 amod airway,cells
R2477 T2853 T2854 amod epithelial,cells
R2478 T2854 T2857 nsubjpass cells,shown
R2479 T2855 T2857 aux have,shown
R2480 T2856 T2857 auxpass been,shown
R2481 T2857 T2857 ROOT shown,shown
R2482 T2858 T2859 aux to,possess
R2483 T2859 T2857 xcomp possess,shown
R2484 T2860 T2862 det a,phenotype
R2485 T2861 T2862 amod hyper-inflammatory,phenotype
R2486 T2862 T2859 dobj phenotype,possess
R2487 T2863 T2859 cc and,possess
R2488 T2864 T2859 conj produce,possess
R2489 T2865 T2869 det an,response
R2490 T2866 T2869 amod exaggerated,response
R2491 T2867 T2869 amod pro-inflammatory,response
R2492 T2868 T2869 compound cytokine,response
R2493 T2869 T2864 dobj response,produce
R2494 T2870 T2872 nmod [,]
R2495 T2871 T2872 nummod 4,]
R2496 T2872 T2869 appos ],response
R2497 T2873 T2872 punct ",",]
R2498 T2874 T2876 nmod [,]
R2499 T2875 T2876 nummod 5,]
R2500 T2876 T2872 appos ],]
R2501 T2877 T2857 punct .,shown
R2502 T2878 T2879 aux To,determine
R2503 T2879 T2903 advcl determine,stimulated
R2504 T2880 T2884 mark if,contribute
R2505 T2881 T2883 amod airway,cells
R2506 T2882 T2883 amod epithelial,cells
R2507 T2883 T2884 nsubj cells,contribute
R2508 T2884 T2903 advcl contribute,stimulated
R2509 T2885 T2884 prep to,contribute
R2510 T2886 T2889 det the,production
R2511 T2887 T2889 amod increased,production
R2512 T2888 T2889 compound IL-1β,production
R2513 T2889 T2885 pobj production,to
R2514 T2890 T2889 prep in,production
R2515 T2891 T2890 pobj patients,in
R2516 T2892 T2891 prep with,patients
R2517 T2893 T2892 pobj CF,with
R2518 T2894 T2893 punct ",",CF
R2519 T2895 T2893 conj CF,CF
R2520 T2896 T2895 cc and,CF
R2521 T2897 T2884 conj control,contribute
R2522 T2898 T2901 amod bronchial,lines
R2523 T2899 T2901 amod epithelial,lines
R2524 T2900 T2901 compound cell,lines
R2525 T2901 T2903 nsubjpass lines,stimulated
R2526 T2902 T2903 auxpass were,stimulated
R2527 T2903 T2903 ROOT stimulated,stimulated
R2528 T2904 T2903 prep with,stimulated
R2529 T2905 T2910 det the,strain
R2530 T2906 T2910 amod inflammasome,strain
R2531 T2907 T2910 nmod inducers,strain
R2532 T2908 T2910 nsubj P.,strain
R2533 T2909 T2910 compound aeruginosa,strain
R2534 T2910 T2904 pobj strain,with
R2535 T2911 T2917 nsubj PAO1,followed
R2536 T2912 T2913 punct (,PAO1
R2537 T2913 T2911 appos PAO1,PAO1
R2538 T2914 T2913 punct ),PAO1
R2539 T2915 T2911 cc and,PAO1
R2540 T2916 T2911 conj LPS,PAO1
R2541 T2917 T2910 relcl followed,strain
R2542 T2918 T2917 prep by,followed
R2543 T2919 T2918 pobj ATP,by
R2544 T2920 T2903 punct .,stimulated
R2545 T2921 T2922 amod IL-1β,levels
R2546 T2922 T2927 nsubj levels,were
R2547 T2923 T2922 prep in,levels
R2548 T2924 T2925 compound cell,culture
R2549 T2925 T2926 compound culture,supernatants
R2550 T2926 T2923 pobj supernatants,in
R2551 T2927 T2930 auxpass were,increased
R2552 T2928 T2930 neg not,increased
R2597 T2973 T2972 pobj contrast,In
R2598 T2974 T2978 punct ",",were
R2599 T2975 T2977 det these,cells
R2600 T2976 T2977 compound airway,cells
R2601 T2977 T2978 nsubj cells,were
R2602 T2978 T2978 ROOT were,were
R2603 T2979 T2980 advmod highly,responsive
R2604 T2980 T2978 acomp responsive,were
R2605 T2981 T2980 prep to,responsive
R2606 T2982 T2984 amod other,stimuli
R2607 T2983 T2984 amod inflammatory,stimuli
R2608 T2984 T2981 pobj stimuli,to
R2609 T2985 T2984 punct ",",stimuli
R2610 T2986 T2987 amod such,as
R2611 T2987 T2984 prep as,stimuli
R2612 T2988 T2989 compound recombinant,IL-1β
R2613 T2989 T2987 pobj IL-1β,as
R2614 T2990 T2978 punct ",",were
R2615 T2991 T2978 advcl producing,were
R2616 T2992 T2993 amod large,quantities
R2617 T2993 T2991 dobj quantities,producing
R2618 T2994 T2993 prep of,quantities
R2619 T2995 T2994 pobj IL-8,of
R2620 T2996 T2993 punct (,quantities
R2621 T2997 T2998 compound Fig.,2a
R2622 T2998 T2993 appos 2a,quantities
R2623 T2999 T3000 nummod d,inserts
R2553 T2929 T2930 advmod greatly,increased
R2554 T2930 T2930 ROOT increased,increased
R2555 T2931 T2930 prep in,increased
R2556 T2932 T2934 det either,CF
R2557 T2933 T2934 det the,CF
R2558 T2934 T2931 pobj CF,in
R2559 T2935 T2934 cc or,CF
R2560 T2936 T2930 xcomp control,increased
R2561 T2937 T2938 compound cell,lines
R2562 T2938 T2936 dobj lines,control
R2563 T2939 T2938 punct (,lines
R2564 T2940 T2941 compound Fig.,2a
R2565 T2941 T2938 appos 2a,lines
R2566 T2942 T2938 appos d,lines
R2567 T2943 T2938 punct ),lines
R2568 T2944 T2936 punct ",",control
R2569 T2945 T2953 mark although,detected
R2570 T2946 T2948 det a,increase
R2571 T2947 T2948 amod small,increase
R2572 T2948 T2953 nsubjpass increase,detected
R2573 T2949 T2948 prep in,increase
R2574 T2950 T2951 amod IL-1β,production
R2575 T2951 T2949 pobj production,in
R2576 T2952 T2953 auxpass was,detected
R2577 T2953 T2936 advcl detected,control
R2578 T2954 T2953 prep in,detected
R2579 T2955 T2954 pobj NuLi-1,in
R2580 T2956 T2955 cc and,NuLi-1
R2581 T2957 T2958 compound CuFi-1,cells
R2582 T2958 T2955 conj cells,NuLi-1
R2583 T2959 T2936 punct ",",control
R2584 T2960 T2936 cc but,control
R2585 T2961 T2962 neg not,in
R2586 T2962 T2936 conj in,control
R2787 T3214 T3216 nmod Airway,cells
R2788 T3215 T3216 amod epithelial,cells
R2789 T3216 T3220 nsubj cells,upregulate
R2790 T3217 T3220 aux do,upregulate
R2791 T3218 T3220 neg not,upregulate
R2792 T3219 T3220 advmod significantly,upregulate
R2793 T3220 T3220 ROOT upregulate,upregulate
R2794 T3221 T3222 compound caspase-1,activity
R2795 T3222 T3220 dobj activity,upregulate
R2796 T3223 T3220 prep in,upregulate
R2797 T3224 T3223 pobj response,in
R2798 T3225 T3224 prep to,response
R2799 T3226 T3227 amod inflammasome,stimulation
R2800 T3227 T3225 pobj stimulation,to
R2801 T3228 T3229 aux To,examine
R2802 T3229 T3220 advcl examine,upregulate
R2803 T3230 T3233 mark if,occurs
R2804 T3231 T3232 amod inflammasome,activation
R2805 T3232 T3233 nsubj activation,occurs
R2806 T3233 T3242 advcl occurs,quantified
R2807 T3234 T3233 prep in,occurs
R2808 T3235 T3237 det these,cells
R2809 T3236 T3237 compound airway,cells
R2810 T3237 T3234 pobj cells,in
R2811 T3238 T3242 punct ",",quantified
R2812 T3239 T3240 amod caspase-1,activity
R2813 T3240 T3242 nsubjpass activity,quantified
R2814 T3241 T3242 auxpass was,quantified
R2815 T3242 T3220 advcl quantified,upregulate
R2816 T3243 T3242 agent by,quantified
R2817 T3244 T3245 compound flow,cytometry
R2818 T3245 T3243 pobj cytometry,by
R2819 T3246 T3242 punct .,quantified
R2820 T3247 T3248 expl There,was
R2821 T3248 T3248 ROOT was,was
R2822 T3249 T3251 det no,increase
R2823 T3250 T3251 amod significant,increase
R2824 T3251 T3248 attr increase,was
R2825 T3252 T3251 prep upon,increase
R2826 T3253 T3252 pobj stimulation,upon
R2827 T3254 T3251 prep with,increase
R2828 T3255 T3256 amod live,PAO1
R2829 T3256 T3254 pobj PAO1,with
R2830 T3257 T3256 cc or,PAO1
R2831 T3258 T3256 conj LPS+ATP,PAO1
R2832 T3259 T3248 prep at,was
R2833 T3260 T3261 det the,times
R2834 T3261 T3259 pobj times,at
R2835 T3262 T3248 conj examined,was
R2836 T3263 T3266 punct (,b
R2837 T3264 T3265 compound Fig.,3a
R2838 T3265 T3266 nummod 3a,b
R2839 T3266 T3262 parataxis b,examined
R2840 T3267 T3266 punct ),b
R2841 T3268 T3248 punct .,was
R2842 T3269 T3273 mark Because,indicated
R2843 T3270 T3271 amod previous,studies
R2844 T3271 T3273 nsubj studies,indicated
R2845 T3272 T3273 aux have,indicated
R2846 T3273 T3295 advcl indicated,examined
R2847 T3274 T3275 det a,role
R2848 T3275 T3273 dobj role,indicated
R2849 T3276 T3275 prep for,role
R2850 T3277 T3276 pobj caspase-1,for
R2851 T3278 T3277 prep in,caspase-1
R2852 T3279 T3280 det the,activation
R2853 T3280 T3278 pobj activation,in
R2854 T3281 T3280 prep of,activation
R2855 T3282 T3281 pobj NF-κB,of
R2856 T3283 T3275 prep through,role
R2857 T3284 T3285 amod Toll-like,receptor
R2858 T3285 T3283 pobj receptor,through
R2859 T3286 T3287 punct (,TLR
R2860 T3287 T3285 appos TLR,receptor
R2861 T3288 T3273 punct ),indicated
R2862 T3289 T3273 advcl signaling,indicated
R2863 T3290 T3292 nmod [,]
R2864 T3291 T3292 nummod 35,]
R2865 T3292 T3289 dobj ],signaling
R2866 T3293 T3295 punct ",",examined
R2867 T3294 T3295 nsubj we,examined
R2868 T3295 T3295 ROOT examined,examined
R2869 T3296 T3305 mark whether,in
R2870 T3297 T3298 compound chemical,inhibition
R2871 T3298 T3305 nsubj inhibition,in
R2872 T3299 T3298 prep of,inhibition
R2873 T3300 T3304 amod caspase-1,production
R2874 T3301 T3304 amod altered,production
R2875 T3302 T3304 nmod NF-κB-dependent,production
R2876 T3303 T3304 compound IL-6,production
R2877 T3304 T3299 pobj production,of
R2878 T3305 T3295 prt in,examined
R2879 T3306 T3305 pobj response,in
R2880 T3307 T3306 prep to,response
R2881 T3308 T3309 compound P.,aeruginosa
R2882 T3309 T3307 pobj aeruginosa,to
R2883 T3310 T3295 punct .,examined
R2884 T3311 T3324 advmod However,decrease
R2885 T3312 T3324 punct ",",decrease
R2886 T3313 T3324 nsubj treatment,decrease
R2887 T3314 T3313 prep with,treatment
R2888 T3315 T3317 det the,inhibitor
R2889 T3316 T3317 amod caspase-1,inhibitor
R2890 T3317 T3314 pobj inhibitor,with
R2891 T3318 T3317 appos z-YVAD-fmk,inhibitor
R2892 T3319 T3320 punct (,YVAD
R2893 T3320 T3318 appos YVAD,z-YVAD-fmk
R2894 T3321 T3318 punct ),z-YVAD-fmk
R2895 T3322 T3324 aux did,decrease
R2896 T3323 T3324 neg not,decrease
R2897 T3324 T3324 ROOT decrease,decrease
R2898 T3325 T3326 compound IL-6,secretion
R2899 T3326 T3324 dobj secretion,decrease
R2900 T3327 T3324 prep by,decrease
R2901 T3328 T3330 nmod airway,cells
R2902 T3329 T3330 amod epithelial,cells
R2903 T3330 T3327 pobj cells,by
R2904 T3331 T3324 punct (,decrease
R2905 T3332 T3324 npadvmod Fig.,decrease
R2906 T3333 T3332 nummod 3c,Fig.
R2907 T3334 T3324 punct ),decrease
R2908 T3335 T3324 punct .,decrease
R3016 T3485 T3487 nummod CD14,monocytes
R3017 T3486 T3487 amod positive,monocytes
R2624 T3000 T2993 appos inserts,quantities
R2625 T3001 T2993 punct ),quantities
R2626 T3002 T2978 punct .,were
R3404 T3916 T3922 nsubj PBMCs,produce
R3405 T3917 T3916 prep from,PBMCs
R3406 T3918 T3919 compound CF,patients
R3407 T3919 T3917 pobj patients,from
R3408 T3920 T3922 aux do,produce
R3409 T3921 T3922 neg not,produce
R3410 T3922 T3922 ROOT produce,produce
R3411 T3923 T3924 amod increased,amounts
R3412 T3924 T3922 dobj amounts,produce
R3413 T3925 T3924 prep of,amounts
R3414 T3926 T3925 pobj IL-1β,of
R3415 T3927 T3922 prep upon,produce
R3416 T3928 T3929 amod inflammasome,stimulation
R3417 T3929 T3931 nmod stimulation,studies
R3418 T3930 T3931 amod Previous,studies
R3419 T3931 T3927 pobj studies,upon
R3420 T3932 T3933 aux have,shown
R3421 T3933 T3922 conj shown,produce
R3422 T3934 T3933 dobj that,shown
R3423 T3935 T3936 det the,loss
R3424 T3936 T3934 nsubj loss,that
R3425 T3937 T3936 prep of,loss
R3426 T3938 T3939 compound CFTR,results
R3427 T3939 T3937 pobj results,of
R3428 T3940 T3939 prep in,results
R3429 T3941 T3943 amod increased,activity
R3430 T3942 T3943 compound NF-κB,activity
R3431 T3943 T3940 pobj activity,in
R3432 T3944 T3943 cc and,activity
R3433 T3945 T3947 amod pro-inflammatory,secretion
R3434 T3946 T3947 compound cytokine,secretion
R3435 T3947 T3943 conj secretion,activity
R3436 T3948 T3950 nmod [,]
R3437 T3949 T3950 nummod 4,]
R3438 T3950 T3947 nmod ],secretion
R3439 T3951 T3950 punct ",",]
R3440 T3952 T3954 nmod [,]
R3441 T3953 T3954 nummod 5,]
R3442 T3954 T3950 conj ],]
R3443 T3955 T3954 punct ",",]
R3444 T3956 T3958 nmod [,]
R3445 T3957 T3958 nummod 32,]
R3446 T3958 T3954 conj ],]
R3018 T3487 T3493 nsubj monocytes,show
R3019 T3488 T3487 prep from,monocytes
R3020 T3489 T3490 compound CF,patients
R3021 T3490 T3488 pobj patients,from
R3022 T3491 T3490 cc and,patients
R3023 T3492 T3490 conj controls,patients
R3024 T3493 T3493 ROOT show,show
R3025 T3494 T3495 amod similar,increases
R3026 T3495 T3493 dobj increases,show
R3027 T3496 T3495 prep in,increases
R3028 T3497 T3498 amod caspase-1,activity
R3029 T3498 T3496 pobj activity,in
R3030 T3499 T3498 prep upon,activity
R3031 T3500 T3501 amod inflammasome,stimulation
R3032 T3501 T3502 compound stimulation,Monocytes
R3033 T3502 T3499 pobj Monocytes,upon
R3034 T3503 T3504 auxpass were,identified
R3035 T3504 T3493 ccomp identified,show
R3036 T3505 T3504 prep in,identified
R3037 T3506 T3507 compound PBMC,populations
R3038 T3507 T3505 pobj populations,in
R3039 T3508 T3504 advcl using,identified
R3040 T3509 T3508 dobj CD14,using
R3041 T3510 T3508 prep as,using
R3042 T3511 T3513 det a,marker
R3043 T3512 T3513 amod phenotyping,marker
R3044 T3513 T3510 pobj marker,as
R3045 T3514 T3493 punct .,show
R3046 T3515 T3517 nummod CD14,monocytes
R3047 T3516 T3517 amod positive,monocytes
R3048 T3517 T3524 nsubj monocytes,showed
R3049 T3518 T3517 prep from,monocytes
R3050 T3519 T3520 compound CF,patients
R3051 T3520 T3518 pobj patients,from
R3052 T3521 T3520 cc and,patients
R3053 T3522 T3523 amod healthy,controls
R3054 T3523 T3520 conj controls,patients
R3055 T3524 T3524 ROOT showed,showed
R3056 T3525 T3527 det a,increase
R3057 T3526 T3527 amod significant,increase
R3058 T3527 T3524 dobj increase,showed
R3059 T3528 T3527 prep in,increase
R3060 T3529 T3530 amod caspase-1,activation
R3061 T3530 T3528 pobj activation,in
R3062 T3531 T3530 prep upon,activation
R3063 T3532 T3531 pobj stimulation,upon
R3064 T3533 T3524 prep with,showed
R3065 T3534 T3533 pobj LPS+ATP,with
R3066 T3535 T3534 punct ",",LPS+ATP
R3067 T3536 T3534 conj PAO1,LPS+ATP
R3068 T3537 T3536 punct ",",PAO1
R3069 T3538 T3536 cc and,PAO1
R3070 T3539 T3540 compound LPS+P,oly
R3071 T3540 T3536 conj oly,PAO1
R3072 T3541 T3544 punct (,dT
R3073 T3542 T3544 nmod dA,dT
R3074 T3543 T3544 punct :,dT
R3075 T3544 T3540 appos dT,oly
R3076 T3545 T3544 punct ),dT
R3077 T3546 T3547 punct (,Fig.
R3078 T3547 T3540 appos Fig.,oly
R3079 T3548 T3547 nummod 4a,Fig.
R3080 T3549 T3540 punct ),oly
R3081 T3550 T3540 cc but,oly
R3082 T3551 T3552 det this,activation
R3083 T3552 T3553 nsubj activation,was
R3084 T3553 T3524 conj was,showed
R3085 T3554 T3553 neg not,was
R3086 T3555 T3553 acomp different,was
R3087 T3556 T3555 prep between,different
R3088 T3557 T3560 nmod CF,subjects
R3089 T3558 T3557 cc and,CF
R3090 T3559 T3557 conj control,CF
R3091 T3560 T3556 pobj subjects,between
R3092 T3561 T3564 punct (,)
R3093 T3562 T3564 nmod Fig.,)
R3094 T3563 T3562 nummod 4b,Fig.
R3095 T3564 T3553 punct ),was
R3096 T3565 T3524 punct .,showed
R3487 T3999 T3983 advcl activate,stimulated
R3488 T4000 T4001 det the,NLRC4
R3489 T4001 T3999 dobj NLRC4,activate
R3490 T4002 T4001 punct ",",NLRC4
R3491 T4003 T4001 conj NLRP3,NLRC4
R3492 T4004 T4003 punct ",",NLRP3
R3493 T4005 T4003 cc and,NLRP3
R3494 T4006 T4003 conj AIM2,NLRP3
R3495 T4007 T4001 appos inflammasomes,NLRC4
R3496 T4008 T4007 punct ",",inflammasomes
R3497 T4009 T4007 advmod respectively,inflammasomes
R3498 T4010 T3983 punct .,stimulated
R3499 T4011 T4021 prep By,produce
R3500 T4012 T4013 nummod 24,hours
R3501 T4013 T4011 pobj hours,By
R3502 T4014 T4013 prep of,hours
R3503 T4015 T4014 pobj stimulation,of
R3504 T4016 T4021 punct ",",produce
R3505 T4017 T4018 compound CF,PBMCs
R3506 T4018 T4021 nsubj PBMCs,produce
R3507 T4019 T4021 aux did,produce
R3508 T4020 T4021 neg not,produce
R3509 T4021 T4021 ROOT produce,produce
R3510 T4022 T4023 amod increased,amounts
R3511 T4023 T4021 dobj amounts,produce
R3512 T4024 T4023 prep of,amounts
R3513 T4025 T4024 pobj IL-1β,of
R3514 T4026 T4023 punct (,amounts
R3515 T4027 T4023 appos Fig.,amounts
R3516 T4028 T4027 nummod 5a,Fig.
R3517 T4029 T4027 punct ),Fig.
R3518 T4030 T4027 cc or,Fig.
R3519 T4031 T4027 conj IL-8,Fig.
R3520 T4032 T4033 punct (,Fig.
R3521 T4033 T4031 appos Fig.,IL-8
R3522 T4034 T4033 nummod 5b,Fig.
R3523 T4035 T4031 punct ),IL-8
R3524 T4036 T4037 advmod when,compared
R3525 T4037 T4021 advcl compared,produce
R3526 T4038 T4037 prep to,compared
R3527 T4039 T4040 amod healthy,controls
R3528 T4040 T4038 pobj controls,to
R3529 T4041 T4021 punct .,produce
R3530 T4042 T4046 advmod However,notice
R3531 T4043 T4046 punct ",",notice
R3532 T4044 T4046 nsubj we,notice
R3533 T4045 T4046 aux did,notice
R3534 T4046 T4046 ROOT notice,notice
R3535 T4047 T4049 det a,decrease
R3536 T4048 T4049 amod transient,decrease
R3537 T4049 T4046 dobj decrease,notice
R3538 T4050 T4051 punct (,P
R3539 T4051 T4049 appos P,decrease
R3540 T4052 T4051 nummod <,P
R3541 T4053 T4051 nummod 0.001,P
R3542 T4054 T4051 punct ),P
R3543 T4055 T4049 prep in,decrease
R3544 T4056 T4057 det the,amount
R3545 T4057 T4055 pobj amount,in
R3546 T4058 T4057 prep of,amount
R3547 T4059 T4074 dobj IL-1β,shown
R3548 T4060 T4059 acl produced,IL-1β
R3549 T4061 T4060 agent by,produced
R3550 T4062 T4063 compound CF,cells
R3551 T4063 T4061 pobj cells,by
R3552 T4064 T4060 prep in,produced
R3553 T4065 T4064 pobj response,in
R3554 T4066 T4065 prep to,response
R3555 T4067 T4066 pobj LPS+ATP,to
R3556 T4068 T4065 prep at,response
R3557 T4069 T4070 nummod 6,hours
R3558 T4070 T4068 pobj hours,at
R3559 T4071 T4074 punct (,shown
R3560 T4072 T4074 nsubj data,shown
R3561 T4073 T4074 neg not,shown
R3562 T4074 T4049 relcl shown,decrease
R3563 T4075 T4049 punct ),decrease
R3564 T4076 T4046 punct .,notice
R3565 T4077 T4098 nsubj Stimulation,produced
R3447 T3959 T3958 punct ",",]
R3448 T3960 T3958 conj [,]
R3449 T3961 T3962 nummod 36,]
R3450 T3962 T3958 conj ],]
R3451 T3963 T3962 punct ",",]
R3452 T3964 T3962 conj [,]
R3453 T3965 T3966 nummod 37,]
R3454 T3966 T3962 conj ],]
R3455 T3967 T3922 punct .,produce
R3456 T3968 T3970 aux To,examine
R3457 T3969 T3970 advmod further,examine
R3458 T3970 T3983 advcl examine,stimulated
R3459 T3971 T3972 det this,relationship
R3460 T3972 T3970 dobj relationship,examine
R3461 T3973 T3983 punct ",",stimulated
R3462 T3974 T3983 nsubjpass PBMCs,stimulated
R3463 T3975 T3974 prep from,PBMCs
R3464 T3976 T3977 compound CF,patients
R3465 T3977 T3975 pobj patients,from
R3466 T3978 T3977 cc and,patients
R3467 T3979 T3981 amod healthy,controls
R3468 T3980 T3981 compound adult,controls
R3469 T3981 T3977 conj controls,patients
R3470 T3982 T3983 auxpass were,stimulated
R3471 T3983 T3983 ROOT stimulated,stimulated
R3472 T3984 T3983 prep with,stimulated
R3473 T3985 T3984 pobj PAO1,with
R3474 T3986 T3985 punct ",",PAO1
R3475 T3987 T3985 conj LPS+ATP,PAO1
R3476 T3988 T3987 punct ",",LPS+ATP
R3477 T3989 T3987 cc and,LPS+ATP
R3478 T3990 T3991 compound LPS+P,oly
R3479 T3991 T3987 conj oly,LPS+ATP
R3480 T3992 T3995 punct (,dT
R3481 T3993 T3995 nmod dA,dT
R3482 T3994 T3995 punct :,dT
R3483 T3995 T3991 appos dT,oly
R3484 T3996 T3995 punct ),dT
R3485 T3997 T3983 punct ",",stimulated
R3486 T3998 T3999 aux to,activate
R4131 T4742 T4739 dobj cells,primed
R4132 T4743 T4739 prep with,primed
R4133 T4744 T4745 compound heat-killed,PAO1
R4134 T4745 T4743 pobj PAO1,with
R4135 T4746 T4739 conj produced,primed
R4136 T4747 T4749 det the,levels
R4137 T4748 T4749 amod highest,levels
R4138 T4749 T4746 dobj levels,produced
R4139 T4750 T4749 prep of,levels
R4140 T4751 T4753 compound NF-κB,AP-1
R4141 T4752 T4753 compound /,AP-1
R4142 T4753 T4754 compound AP-1,activation
R3566 T4078 T4077 prep of,Stimulation
R3567 T4079 T4078 pobj PBMCs,of
R3568 T4080 T4079 prep with,PBMCs
R3569 T4081 T4082 compound P.,aeruginosa
R3570 T4082 T4080 pobj aeruginosa,with
R3571 T4083 T4084 nsubj that,lacks
R3572 T4084 T4082 relcl lacks,aeruginosa
R3573 T4085 T4084 dobj exsA,lacks
R3574 T4086 T4087 punct (,PAO1ΔexsA
R3575 T4087 T4085 appos PAO1ΔexsA,exsA
R3576 T4088 T4077 punct ),Stimulation
R3577 T4089 T4077 punct ",",Stimulation
R3578 T4090 T4092 det a,regulator
R3579 T4091 T4092 amod key,regulator
R3580 T4092 T4077 appos regulator,Stimulation
R3581 T4093 T4092 prep of,regulator
R3582 T4094 T4096 compound type,secretion
R3583 T4095 T4096 compound III,secretion
R3584 T4096 T4093 pobj secretion,of
R3585 T4097 T4098 punct ",",produced
R3586 T4098 T4098 ROOT produced,produced
R3587 T4099 T4101 amod three-fold,IL-1β
R3588 T4100 T4101 advmod less,IL-1β
R3589 T4101 T4102 amod IL-1β,compared
R3590 T4102 T4098 prep compared,produced
R3591 T4103 T4102 prep to,compared
R3592 T4104 T4107 det the,strain
R3593 T4105 T4107 amod parental,strain
R3594 T4106 T4107 compound PAO1,strain
R3595 T4107 T4103 pobj strain,to
R3596 T4108 T4107 prep by,strain
R3597 T4109 T4110 nummod 24,hours
R3598 T4110 T4108 pobj hours,by
R3599 T4111 T4110 punct (,hours
R3600 T4112 T4110 appos Fig.,hours
R3601 T4113 T4112 nummod 5a,Fig.
R3602 T4114 T4110 punct ),hours
R3603 T4115 T4098 punct .,produced
R3604 T4116 T4117 amod Inflammasome,stimulation
R3605 T4117 T4122 nsubj stimulation,result
R3606 T4118 T4117 prep without,stimulation
R3607 T4119 T4118 pcomp priming,without
R3608 T4120 T4122 aux did,result
R3609 T4121 T4122 neg not,result
R3610 T4122 T4122 ROOT result,result
R3611 T4123 T4122 prep in,result
R3612 T4124 T4126 det any,production
R3613 T4125 T4126 amod IL-1β,production
R3614 T4126 T4123 pobj production,in
R3615 T4127 T4126 prep in,production
R3616 T4128 T4129 det either,CF
R3617 T4129 T4127 pobj CF,in
R3618 T4130 T4129 cc or,CF
R3619 T4131 T4132 compound control,PBMCs
R3620 T4132 T4129 conj PBMCs,CF
R3621 T4133 T4122 punct .,result
R3622 T4134 T4141 advcl Contrary,indicate
R3623 T4135 T4134 prep to,Contrary
R3624 T4136 T4137 poss our,hypothesis
R3625 T4137 T4135 pobj hypothesis,to
R3626 T4138 T4141 punct ",",indicate
R3627 T4139 T4140 det these,results
R3628 T4140 T4141 nsubj results,indicate
R3629 T4141 T4141 ROOT indicate,indicate
R3630 T4142 T4149 mark that,display
R3631 T4143 T4149 nsubj PBMCs,display
R3632 T4144 T4143 prep from,PBMCs
R3633 T4145 T4146 compound CF,patients
R3634 T4146 T4144 pobj patients,from
R3635 T4147 T4149 aux do,display
R3636 T4148 T4149 neg not,display
R3637 T4149 T4141 ccomp display,indicate
R3638 T4150 T4151 amod increased,production
R3639 T4151 T4149 dobj production,display
R3640 T4152 T4151 prep of,production
R3641 T4153 T4152 pobj IL-1β,of
R3642 T4154 T4153 cc or,IL-1β
R3643 T4155 T4153 conj IL-8,IL-1β
R3644 T4156 T4149 prep with,display
R3645 T4157 T4158 amod inflammasome,activation
R3646 T4158 T4156 pobj activation,with
R3647 T4159 T4149 cc nor,display
R3648 T4160 T4162 aux do,suggest
R3649 T4161 T4162 nsubj they,suggest
R3650 T4162 T4149 conj suggest,display
R3651 T4163 T4165 det any,basal
R3652 T4164 T4165 amod increased,basal
R3653 T4165 T4162 dobj basal,suggest
R3654 T4166 T4165 cc or,basal
R3655 T4167 T4169 amod induced,activity
R3656 T4168 T4169 compound NF-κB,activity
R3657 T4169 T4165 conj activity,basal
R3658 T4170 T4141 punct .,indicate
R3659 T4171 T4172 det These,results
R3660 T4172 T4173 nsubj results,are
R3661 T4173 T4173 ROOT are,are
R3662 T4174 T4173 acomp consistent,are
R3663 T4175 T4174 prep with,consistent
R3664 T4176 T4177 poss our,observation
R3665 T4177 T4175 pobj observation,with
R3666 T4178 T4181 mark that,is
R3667 T4179 T4180 amod caspase-1,activity
R3668 T4180 T4181 nsubj activity,is
R3669 T4181 T4173 advcl is,are
R3670 T4182 T4181 neg not,is
R3671 T4183 T4181 acomp different,is
R3672 T4184 T4183 prep between,different
R3673 T4185 T4184 pobj CF,between
R3674 T4186 T4185 cc and,CF
R3675 T4187 T4188 compound control,PBMCs
R3676 T4188 T4185 conj PBMCs,CF
R3677 T4189 T4190 punct (,Fig.
R3678 T4190 T4188 appos Fig.,PBMCs
R3679 T4191 T4190 nummod 4,Fig.
R3680 T4192 T4188 punct ),PBMCs
R3681 T4193 T4173 punct .,are
R4084 T4695 T4696 amod NF-κB,activation
R4085 T4696 T4698 nsubjpass activation,required
R4086 T4697 T4698 auxpass is,required
R4087 T4698 T4698 ROOT required,required
R4088 T4699 T4698 prep for,required
R4089 T4700 T4699 pobj IL-1β,for
R4090 T4701 T4700 cc and,IL-1β
R4091 T4702 T4700 conj IL-8,IL-1β
R4092 T4703 T4708 npadvmod responses,confirmed
R4093 T4704 T4703 prep to,responses
R4094 T4705 T4706 compound P.,aeruginosa
R4095 T4706 T4704 pobj aeruginosa,to
R4096 T4707 T4708 nsubj We,confirmed
R4097 T4708 T4698 advcl confirmed,required
R4098 T4709 T4710 det the,dependence
R4099 T4710 T4716 nmod dependence,production
R4100 T4711 T4710 prep of,dependence
R4101 T4712 T4713 amod PAO1-induced,IL-1β
R4102 T4713 T4711 pobj IL-1β,of
R4103 T4714 T4713 cc and,IL-1β
R4104 T4715 T4716 amod IL-8,production
R4105 T4716 T4708 dobj production,confirmed
R4106 T4717 T4716 prep on,production
R4107 T4718 T4719 amod NF-κB,activation
R4108 T4719 T4717 pobj activation,on
R4109 T4720 T4708 advcl using,confirmed
R4110 T4721 T4722 nummod THP-1,cells
R4111 T4722 T4720 dobj cells,using
R4112 T4723 T4722 acl expressing,cells
R4113 T4724 T4725 det a,reporter
R4114 T4725 T4723 dobj reporter,expressing
R4115 T4726 T4725 acl driven,reporter
R4116 T4727 T4726 agent by,driven
R4117 T4728 T4732 nmod NF-κB,elements
R4118 T4729 T4728 cc and,NF-κB
R4119 T4730 T4728 conj AP-1,NF-κB
R4120 T4731 T4732 compound response,elements
R4121 T4732 T4727 pobj elements,by
R4122 T4733 T4698 punct .,required
R4123 T4734 T4735 nsubj We,found
R4124 T4735 T4735 ROOT found,found
R4125 T4736 T4739 mark that,primed
R4143 T4754 T4750 pobj activation,of
R4144 T4755 T4756 punct (,Fig.
R4232 T4843 T4845 nsubj activation,is
R4233 T4844 T4843 advmod alone,activation
R4234 T4845 T4840 ccomp is,confirmed
R4235 T4846 T4845 neg not,is
R4236 T4847 T4845 acomp sufficient,is
R4237 T4848 T4847 prep for,sufficient
R4238 T4849 T4851 amod maximal,secretion
R4239 T4850 T4851 amod IL-1β,secretion
R4240 T4851 T4848 pobj secretion,for
R4241 T4852 T4840 punct ",",confirmed
R4242 T4853 T4840 cc but,confirmed
R4243 T4854 T4840 conj increased,confirmed
R4244 T4855 T4854 dobj priming,increased
R4245 T4856 T4855 prep of,priming
R4246 T4857 T4856 pobj NF-κB,of
R4247 T4858 T4840 conj is,confirmed
R4248 T4859 T4858 acomp capable,is
R4249 T4860 T4859 prep of,capable
R4250 T4861 T4860 pcomp augmenting,of
R4251 T4862 T4863 amod IL-1β,production
R4252 T4863 T4861 dobj production,augmenting
R4253 T4864 T4863 cc and,production
R4254 T4865 T4863 conj secretion,production
R4255 T4866 T4863 prep upon,production
R4256 T4867 T4868 amod inflammasome,stimulation
R4257 T4868 T4866 pobj stimulation,upon
R4258 T4869 T4858 punct .,is
R4259 T4870 T4877 nsubjpass Dependency,confirmed
R4260 T4871 T4870 prep of,Dependency
R4261 T4872 T4873 det these,responses
R4262 T4873 T4871 pobj responses,of
R4263 T4874 T4870 prep on,Dependency
R4264 T4875 T4874 pobj NF-κB,on
R4265 T4876 T4877 auxpass was,confirmed
R4266 T4877 T4877 ROOT confirmed,confirmed
R4267 T4878 T4877 agent by,confirmed
R4268 T4879 T4880 amod pharmacologic,inhibition
R4269 T4880 T4878 pobj inhibition,by
R4270 T4881 T4880 prep of,inhibition
R4271 T4882 T4881 pobj NF-κB,of
R4272 T4883 T4877 advcl using,confirmed
R4273 T4884 T4886 det the,inhibitor
R4274 T4885 T4886 amod Bay11-7082,inhibitor
R4275 T4886 T4883 dobj inhibitor,using
R4276 T4887 T4886 prep of,inhibitor
R4277 T4888 T4889 compound IκBα,phosphorylation
R4278 T4889 T4887 pobj phosphorylation,of
R4279 T4890 T4889 punct ",",phosphorylation
R4280 T4891 T4893 nsubj which,reduced
R4126 T4737 T4739 nsubj stimulation,primed
R4127 T4738 T4737 prep of,stimulation
R4128 T4739 T4735 ccomp primed,found
R4129 T4740 T4742 nummod THP-1,cells
R4130 T4741 T4742 compound reporter,cells
R4145 T4756 T4749 appos Fig.,levels
R4146 T4757 T4756 nummod 6a,Fig.
R4147 T4758 T4756 punct ),Fig.
R4148 T4759 T4739 cc and,primed
R4149 T4760 T4761 nsubj this,correlated
R4150 T4761 T4739 conj correlated,primed
R4151 T4762 T4761 prep with,correlated
R4152 T4763 T4764 amod IL-8,secretion
R4153 T4764 T4762 pobj secretion,with
R4154 T4765 T4764 punct (,secretion
R4155 T4766 T4764 appos Fig.,secretion
R4156 T4767 T4766 nummod 6b,Fig.
R4157 T4768 T4764 punct ),secretion
R4158 T4769 T4761 cc but,correlated
R4159 T4770 T4771 amod negligible,amounts
R4160 T4771 T4775 nsubjpass amounts,secreted
R4161 T4772 T4771 prep of,amounts
R4162 T4773 T4772 pobj IL-1β,of
R4163 T4774 T4775 auxpass were,secreted
R4164 T4775 T4739 conj secreted,primed
R4165 T4776 T4775 punct (,secreted
R4166 T4777 T4779 nmod Fig.,)
R4167 T4778 T4777 nummod 6c,Fig.
R4168 T4779 T4775 punct ),secreted
R4169 T4780 T4775 punct .,secreted
R4170 T4781 T4783 nsubj Stimulation,primed
R4171 T4782 T4781 prep of,Stimulation
R4172 T4783 T4783 ROOT primed,primed
R4173 T4784 T4786 nummod THP-1,cells
R4174 T4785 T4786 compound reporter,cells
R4175 T4786 T4783 dobj cells,primed
R4176 T4787 T4783 prep with,primed
R4177 T4788 T4789 amod live,PAO1
R4178 T4789 T4787 pobj PAO1,with
R4179 T4790 T4793 aux did,increase
R4180 T4791 T4793 neg not,increase
R4181 T4792 T4793 advmod significantly,increase
R4182 T4793 T4783 advcl increase,primed
R4183 T4794 T4797 amod NF-κB,activity
R4184 T4795 T4797 nmod /,activity
R4185 T4796 T4797 compound AP-1,activity
R4186 T4797 T4793 dobj activity,increase
R4187 T4798 T4804 punct (,secretion
R4188 T4799 T4804 nmod Fig.,secretion
R4189 T4800 T4799 nummod 6a,Fig.
R4190 T4801 T4799 punct ),Fig.
R4191 T4802 T4799 cc or,Fig.
R4192 T4803 T4804 amod IL-8,secretion
R4193 T4804 T4793 dep secretion,increase
R4194 T4805 T4804 punct (,secretion
R4195 T4806 T4804 appos Fig.,secretion
R4196 T4807 T4806 nummod 6b,Fig.
R4197 T4808 T4804 punct ),secretion
R4198 T4809 T4793 prep over,increase
R4199 T4810 T4809 pcomp priming,over
R4200 T4811 T4810 advmod alone,priming
R4201 T4812 T4783 punct .,primed
R4202 T4813 T4819 advmod However,augmented
R4203 T4814 T4819 punct ",",augmented
R4204 T4815 T4816 amod IL-1β,production
R4205 T4816 T4819 nsubjpass production,augmented
R4206 T4817 T4819 auxpass was,augmented
R4207 T4818 T4819 advmod greatly,augmented
R4208 T4819 T4819 ROOT augmented,augmented
R4209 T4820 T4819 prep over,augmented
R4210 T4821 T4822 amod primed,cells
R4211 T4822 T4820 pobj cells,over
R4212 T4823 T4822 acl stimulated,cells
R4213 T4824 T4823 prep with,stimulated
R4214 T4825 T4829 amod heat-killed,cells
R4215 T4826 T4829 nummod PAO1,cells
R4216 T4827 T4826 cc or,PAO1
R4217 T4828 T4829 amod unprimed,cells
R4218 T4829 T4824 pobj cells,with
R4219 T4830 T4829 acl stimulated,cells
R4220 T4831 T4830 prep with,stimulated
R4221 T4832 T4833 amod live,PAO1
R4222 T4833 T4831 pobj PAO1,with
R4223 T4834 T4833 punct (,PAO1
R4224 T4835 T4833 appos Fig.,PAO1
R4225 T4836 T4835 nummod 6c,Fig.
R4226 T4837 T4833 punct ),PAO1
R4227 T4838 T4819 punct .,augmented
R4228 T4839 T4840 nsubj This,confirmed
R4229 T4840 T4840 ROOT confirmed,confirmed
R4230 T4841 T4845 mark that,is
R4231 T4842 T4843 amod NF-κB,activation
R4282 T4893 T4886 relcl reduced,inhibitor
R4283 T4894 T4896 compound NF-κB,AP-1
R4284 T4895 T4896 compound /,AP-1
R4285 T4896 T4897 compound AP-1,activation
R4286 T4897 T4893 dobj activation,reduced
R4287 T4898 T4899 punct (,P
R4288 T4899 T4897 appos P,activation
R4289 T4900 T4899 nummod <,P
R4290 T4901 T4899 nummod 0.001,P
R4291 T4902 T4899 punct ),P
R4292 T4903 T4899 punct (,P
R4293 T4904 T4899 appos Fig.,P
R4294 T4905 T4904 nummod 6d,Fig.
R4295 T4906 T4899 punct ),P
R4296 T4907 T4897 cc and,activation
R4297 T4908 T4910 det the,production
R4298 T4909 T4910 amod subsequent,production
R4299 T4910 T4897 conj production,activation
R4300 T4911 T4910 prep of,production
R4301 T4912 T4911 pobj IL-8,of
R4302 T4913 T4912 punct (,IL-8
R4303 T4914 T4912 appos P,IL-8
R4304 T4915 T4916 compound <,0.01
R4305 T4916 T4914 nummod 0.01,P
R4306 T4917 T4912 punct ),IL-8
R4307 T4918 T4910 punct (,production
R4308 T4919 T4910 appos Fig.,production
R4309 T4920 T4919 nummod 6e,Fig.
R4310 T4921 T4910 punct ),production
R4311 T4922 T4910 cc and,production
R4312 T4923 T4930 nmod IL-1β,Fig.
R4313 T4924 T4925 punct (,P
R4314 T4925 T4930 nmod P,Fig.
R4315 T4926 T4925 nummod <,P
R4316 T4927 T4925 nummod 0.001,P
R4317 T4928 T4925 punct ),P
R4318 T4929 T4930 punct (,Fig.
R4319 T4930 T4886 appos Fig.,inhibitor
R4320 T4931 T4930 nummod 6f,Fig.
R4321 T4932 T4886 punct ),inhibitor
R4322 T4933 T4883 prep in,using
R4323 T4934 T4933 pobj response,in
R4324 T4935 T4934 prep to,response
R4325 T4936 T4937 preconj both,heat-killed
R4326 T4937 T4940 amod heat-killed,PAO1
R4327 T4938 T4937 cc and,heat-killed
R4328 T4939 T4937 conj live,heat-killed
R4329 T4940 T4935 pobj PAO1,to
R4330 T4941 T4877 punct .,confirmed
R4331 T4942 T4943 det These,results
R4332 T4943 T4946 nsubjpass results,verified
R4333 T4944 T4946 auxpass were,verified
R4334 T4945 T4946 advmod also,verified
R4335 T4946 T4946 ROOT verified,verified
R4336 T4947 T4946 prep in,verified
R4337 T4948 T4947 pobj CF,in
R4338 T4949 T4948 cc and,CF
R4339 T4950 T4951 compound control,PBMCs
R4340 T4951 T4946 oprd PBMCs,verified
R4341 T4952 T4951 prep for,PBMCs
R4342 T4953 T4954 det each,inflammasome
R4343 T4954 T4955 amod inflammasome,examined
R4344 T4955 T4952 pobj examined,for
R4345 T4956 T4951 punct (,PBMCs
R4346 T4957 T4951 appos P,PBMCs
R4347 T4958 T4957 nummod <,P
R4281 T4892 T4893 advmod significantly,reduced
R4609 T5326 T5327 amod Bay11-7082,inhibits
R4610 T5327 T5327 ROOT inhibits,inhibits
R4611 T5328 T5329 compound pro-IL-1β,production
R4612 T5329 T5327 dobj production,inhibits
R4613 T5330 T5327 prep in,inhibits
R4614 T5331 T5330 pobj response,in
R4615 T5332 T5331 prep to,response
R4616 T5333 T5334 compound P.,aeruginosa
R4617 T5334 T5332 pobj aeruginosa,to
R4618 T5335 T5347 prep In,inhibit
R4619 T5336 T5335 pobj addition,In
R4620 T5337 T5336 prep to,addition
R4621 T5338 T5337 pobj inhibition,to
R4622 T5339 T5338 prep of,inhibition
R4623 T5340 T5341 amod NF-κB,activity
R4624 T5341 T5339 pobj activity,of
R4625 T5342 T5347 punct ",",inhibit
R4626 T5343 T5347 nsubj Bay11-7082,inhibit
R4627 T5344 T5347 aux can,inhibit
R4628 T5345 T5347 advmod also,inhibit
R4629 T5346 T5347 advmod directly,inhibit
R4630 T5347 T5347 ROOT inhibit,inhibit
R4631 T5348 T5351 det the,[
R4632 T5349 T5351 nmod NLRP3,[
R4633 T5350 T5351 compound inflammasome,[
R4709 T5426 T5425 punct ",",0.5
R4710 T5427 T5425 conj 1,0.5
R4711 T5428 T5421 punct ",",inhibit
R4712 T5429 T5421 cc and,inhibit
R4713 T5430 T5431 nummod 1.5,hours
R4714 T5431 T5432 compound hours,post
R4715 T5432 T5421 conj post,inhibit
R4716 T5433 T5434 nummod PAO1,stimulation
R4717 T5434 T5432 dobj stimulation,post
R4718 T5435 T5432 punct (,post
R4719 T5436 T5438 nmod Fig.,)
R4720 T5437 T5436 nummod 7b,Fig.
R4721 T5438 T5432 punct ),post
R4722 T5439 T5415 punct .,corroborated
R4934 T5689 T5687 pobj patients,from
R4935 T5690 T5689 cc and,patients
R4936 T5691 T5692 amod healthy,controls
R4937 T5692 T5689 conj controls,patients
R4938 T5693 T5694 auxpass were,treated
R4939 T5694 T5694 ROOT treated,treated
R4940 T5695 T5694 prep with,treated
R4941 T5696 T5698 det the,inhibitor
R4942 T5697 T5698 compound CFTR,inhibitor
R4943 T5698 T5695 pobj inhibitor,with
R4944 T5699 T5698 punct ",",inhibitor
R4348 T4959 T4957 appos 0.001,P
R4349 T4960 T4951 punct ),PBMCs
R4350 T4961 T4951 punct (,PBMCs
R4351 T4962 T4963 compound Fig.,6g
R4352 T4963 T4951 appos 6g,PBMCs
R4353 T4964 T4951 appos h,PBMCs
R4354 T4965 T4951 punct ),PBMCs
R4355 T4966 T4946 punct .,verified
R4356 T4967 T4970 advmod Overall,confirm
R4357 T4968 T4969 det these,results
R4358 T4969 T4970 nsubj results,confirm
R4359 T4970 T4970 ROOT confirm,confirm
R4360 T4971 T4973 mark that,is
R4361 T4972 T4973 nsubj NF-κB,is
R4362 T4973 T4970 ccomp is,confirm
R4363 T4974 T4976 det an,modulator
R4364 T4975 T4976 amod important,modulator
R4365 T4976 T4973 attr modulator,is
R4366 T4977 T4976 prep of,modulator
R4367 T4978 T4979 amod IL-1β,production
R4368 T4979 T4977 pobj production,of
R4369 T4980 T4973 cc and,is
R4370 T4981 T4983 det that,activation
R4371 T4982 T4983 amod increased,activation
R4372 T4983 T4973 conj activation,is
R4373 T4984 T4983 prep of,activation
R4374 T4985 T4986 amod NF-κB,augments
R4375 T4986 T4984 pobj augments,of
R4376 T4987 T4988 amod inflammasome-mediated,production
R4377 T4988 T4986 dobj production,augments
R4378 T4989 T4988 prep of,production
R4379 T4990 T4989 pobj IL-1β,of
R4380 T4991 T4970 punct .,confirm
R4634 T5351 T5347 dobj [,inhibit
R4635 T5352 T5353 nummod 38,]
R4636 T5353 T5351 appos ],[
R4637 T5354 T5347 punct .,inhibit
R4638 T5355 T5356 aux To,validate
R4639 T5356 T5372 advcl validate,performed
R4640 T5357 T5358 poss its,use
R4641 T5358 T5356 dobj use,validate
R4642 T5359 T5358 prep in,use
R4643 T5360 T5361 det this,study
R4644 T5361 T5359 pobj study,in
R4645 T5362 T5356 prep as,validate
R4646 T5363 T5365 det an,inhibitor
R4647 T5364 T5365 amod NF-κB,inhibitor
R4648 T5365 T5362 pobj inhibitor,as
R4649 T5366 T5365 punct ",",inhibitor
R4650 T5367 T5368 amod western,blots
R4651 T5368 T5372 nsubjpass blots,performed
R4652 T5369 T5368 prep for,blots
R4653 T5370 T5369 pobj pro-IL-1β,for
R4654 T5371 T5372 auxpass were,performed
R4655 T5372 T5372 ROOT performed,performed
R4656 T5373 T5372 prep alongside,performed
R4657 T5374 T5373 pobj inhibition,alongside
R4658 T5375 T5374 prep of,inhibition
R4659 T5376 T5377 amod CFTR,activity
R4660 T5377 T5375 pobj activity,of
R4661 T5378 T5372 agent by,performed
R4662 T5379 T5378 pobj CFTRinh172,by
R4663 T5380 T5372 prep in,performed
R4664 T5381 T5380 pobj response,in
R4665 T5382 T5381 prep to,response
R4666 T5383 T5382 pobj PAO1,to
R4667 T5384 T5372 prep at,performed
R4668 T5385 T5386 nummod 4,hours
R4669 T5386 T5387 npadvmod hours,after
R4670 T5387 T5372 prep after,performed
R4671 T5388 T5387 pobj stimulation,after
R4672 T5389 T5372 punct (,performed
R4673 T5390 T5392 nmod Fig.,)
R4674 T5391 T5390 nummod 7a,Fig.
R4675 T5392 T5372 punct ),performed
R4676 T5393 T5372 punct .,performed
R4677 T5394 T5395 poss Our,results
R4678 T5395 T5396 nsubj results,indicate
R4679 T5396 T5396 ROOT indicate,indicate
R4680 T5397 T5399 mark that,prevents
R4681 T5398 T5399 nsubj Bay11-7082,prevents
R4682 T5399 T5396 ccomp prevents,indicate
R4683 T5400 T5399 dobj production,prevents
R4684 T5401 T5400 prep of,production
R4685 T5402 T5401 pobj pro-IL-1β,of
R4686 T5403 T5407 mark whereas,seem
R4687 T5404 T5407 nsubj CFTRinh172,seem
R4688 T5405 T5407 aux does,seem
R4689 T5406 T5407 neg not,seem
R4690 T5407 T5399 advcl seem,prevents
R4691 T5408 T5409 aux to,affect
R4692 T5409 T5407 xcomp affect,seem
R4693 T5410 T5409 dobj it,affect
R4694 T5411 T5396 punct .,indicate
R4695 T5412 T5415 nsubjpass This,corroborated
R4696 T5413 T5415 auxpass was,corroborated
R4697 T5414 T5415 advmod further,corroborated
R4698 T5415 T5415 ROOT corroborated,corroborated
R4699 T5416 T5415 agent by,corroborated
R4700 T5417 T5418 det the,ability
R4701 T5418 T5416 pobj ability,by
R4702 T5419 T5418 amod Bay11-7082,ability
R4703 T5420 T5421 aux to,inhibit
R4704 T5421 T5418 acl inhibit,ability
R4705 T5422 T5423 compound IκBα,degradation
R4706 T5423 T5421 dobj degradation,inhibit
R4707 T5424 T5423 prep at,degradation
R4708 T5425 T5424 pobj 0.5,at
R4884 T5639 T5645 nsubj Disruption,increase
R4885 T5640 T5639 prep of,Disruption
R4886 T5641 T5642 amod CFTR,activity
R4887 T5642 T5640 pobj activity,of
R4888 T5643 T5645 aux does,increase
R4889 T5644 T5645 neg not,increase
R4890 T5645 T5645 ROOT increase,increase
R4891 T5646 T5647 amod IL-1β,production
R4892 T5647 T5645 dobj production,increase
R4893 T5648 T5647 prep in,production
R4894 T5649 T5648 pobj PBMCs,in
R4895 T5650 T5649 cc and,PBMCs
R4896 T5651 T5649 conj THP-1,PBMCs
R4897 T5652 T5647 conj cells,production
R4898 T5653 T5655 det A,study
R4899 T5654 T5655 amod previous,study
R4900 T5655 T5657 nsubj study,indicated
R4901 T5656 T5657 aux has,indicated
R4902 T5657 T5645 conj indicated,increase
R4903 T5658 T5659 det a,role
R4904 T5659 T5657 dobj role,indicated
R4905 T5660 T5659 prep for,role
R4906 T5661 T5663 compound chloride,concentration
R4907 T5662 T5663 compound ion,concentration
R4908 T5663 T5660 pobj concentration,for
R4909 T5664 T5663 prep in,concentration
R4910 T5665 T5664 pobj suppression,in
R4911 T5666 T5665 prep of,suppression
R4912 T5667 T5669 nmod NLRP3,activation
R4913 T5668 T5669 amod inflammasome,activation
R4914 T5669 T5666 pobj activation,of
R4915 T5670 T5664 pobj [,in
R4916 T5671 T5672 nummod 34,]
R4917 T5672 T5670 appos ],[
R4918 T5673 T5657 punct .,indicated
R4919 T5674 T5675 aux To,determine
R4920 T5675 T5694 advcl determine,treated
R4921 T5676 T5679 mark whether,alters
R4922 T5677 T5678 amod CFTR,dysfunction
R4923 T5678 T5679 nsubj dysfunction,alters
R4924 T5679 T5675 ccomp alters,determine
R4925 T5680 T5681 amod IL-1β,production
R4926 T5681 T5694 nsubjpass production,treated
R4927 T5682 T5681 punct ",",production
R4928 T5683 T5684 nummod THP-1,cells
R4929 T5684 T5681 conj cells,production
R4930 T5685 T5684 cc and,cells
R4931 T5686 T5684 conj PBMCs,cells
R4932 T5687 T5681 prep from,production
R4933 T5688 T5689 compound CF,patients
R4945 T5700 T5698 appos CFTRinh172,inhibitor
R4946 T5701 T5698 punct ",",inhibitor
R4947 T5702 T5694 advmod prior,treated
R4948 T5703 T5702 prep to,prior
R4949 T5704 T5703 pobj simulation,to
R4950 T5705 T5694 prep with,treated
R4951 T5706 T5708 amod live,aeruginosa
R4952 T5707 T5708 compound P.,aeruginosa
R4953 T5708 T5705 pobj aeruginosa,with
R4954 T5709 T5694 punct .,treated
R4955 T5710 T5715 nsubj Treatment,alter
R4956 T5711 T5710 prep with,Treatment
R4957 T5712 T5711 pobj CFTRinh172,with
R4958 T5713 T5715 aux did,alter
R4959 T5714 T5715 neg not,alter
R6548 T7458 T7434 conj are,increased
R6549 T7459 T7458 advmod still,are
R6550 T7460 T7458 acomp unclear,are
R6551 T7461 T7458 punct .,are
R6552 T7462 T7464 nsubj We,studied
R6553 T7463 T7464 advmod first,studied
R6554 T7464 T7464 ROOT studied,studied
R6555 T7465 T7467 nmod airway,cells
R6556 T7466 T7467 amod epithelial,cells
R6557 T7467 T7464 dobj cells,studied
R6558 T7468 T7467 amod due,cells
R6559 T7469 T7468 pcomp to,due
R6560 T7470 T7471 poss their,role
R6561 T7471 T7468 pobj role,due
R6562 T7472 T7471 prep in,role
R6563 T7473 T7474 compound barrier,function
R6564 T7474 T7472 pobj function,in
R6565 T7475 T7471 punct ",",role
R6566 T7476 T7471 conj proximity,role
R6567 T7477 T7476 prep to,proximity
R6568 T7478 T7477 pobj infection,to
R6569 T7479 T7476 punct ",",proximity
R6570 T7480 T7476 cc and,proximity
R6571 T7481 T7476 conj ability,proximity
R6572 T7482 T7483 aux to,produce
R6573 T7483 T7481 acl produce,ability
R6574 T7484 T7485 amod high,levels
R6575 T7485 T7483 dobj levels,produce
R6576 T7486 T7485 prep of,levels
R6577 T7487 T7488 amod pro-inflammatory,cytokines
R6578 T7488 T7486 pobj cytokines,of
R6579 T7489 T7464 punct .,studied
R6580 T7490 T7493 advmod However,found
R6581 T7491 T7493 punct ",",found
R6582 T7492 T7493 nsubj we,found
R6583 T7493 T7493 ROOT found,found
R6584 T7494 T7500 mark that,produce
R6585 T7495 T7497 amod bronchial,cells
R6586 T7496 T7497 amod epithelial,cells
R6587 T7497 T7500 nsubj cells,produce
R6588 T7498 T7500 aux do,produce
R4960 T5715 T5715 ROOT alter,alter
R4961 T5716 T5719 amod IL-1β,production
R4962 T5717 T5716 cc or,IL-1β
R4963 T5718 T5716 conj IL-8,IL-1β
R4964 T5719 T5715 dobj production,alter
R4965 T5720 T5719 prep in,production
R4966 T5721 T5722 compound control,subjects
R4967 T5722 T5720 pobj subjects,in
R4968 T5723 T5722 cc or,subjects
R4969 T5724 T5725 compound CF,patients
R4970 T5725 T5722 conj patients,subjects
R4971 T5726 T5725 punct (,patients
R4972 T5727 T5728 compound Fig.,8a
R4973 T5728 T5725 appos 8a,patients
R4974 T5729 T5725 appos b,patients
R4975 T5730 T5725 punct ),patients
R4976 T5731 T5715 punct .,alter
R4977 T5732 T5736 advmod Similarly,was
R4978 T5733 T5736 punct ",",was
R4979 T5734 T5735 compound IL-1β,production
R4980 T5735 T5736 nsubj production,was
R4981 T5736 T5736 ROOT was,was
R4982 T5737 T5736 neg not,was
R4983 T5738 T5736 acomp different,was
R4984 T5739 T5738 prep in,different
R4985 T5740 T5741 amod monocyte-derived,macrophages
R4986 T5741 T5739 pobj macrophages,in
R4987 T5742 T5741 cc or,macrophages
R4988 T5743 T5745 nummod THP-1,cells
R4989 T5744 T5745 compound reporter,cells
R4990 T5745 T5741 conj cells,macrophages
R4991 T5746 T5741 acl treated,macrophages
R4992 T5747 T5746 prep with,treated
R4993 T5748 T5747 pobj CFTRinh172,with
R4994 T5749 T5748 punct (,CFTRinh172
R4995 T5750 T5748 appos Fig.,CFTRinh172
R4996 T5751 T5750 nummod 8c,Fig.
R4997 T5752 T5746 npadvmod d,treated
R4998 T5753 T5752 punct ),d
R4999 T5754 T5736 punct .,was
R5000 T5755 T5767 nsubj IL-8,were
R5001 T5756 T5757 punct (,Fig.
R5002 T5757 T5755 appos Fig.,IL-8
R5003 T5758 T5757 nummod 8e,Fig.
R5004 T5759 T5757 punct ),Fig.
R5005 T5760 T5755 cc and,IL-8
R5006 T5761 T5762 amod NF-κB,activity
R5007 T5762 T5755 conj activity,IL-8
R5008 T5763 T5755 punct (,IL-8
R5009 T5764 T5755 appos Fig.,IL-8
R5010 T5765 T5764 nummod 8f,Fig.
R5011 T5766 T5755 punct ),IL-8
R5012 T5767 T5767 ROOT were,were
R5013 T5768 T5767 advmod also,were
R5014 T5769 T5767 acomp unchanged,were
R5015 T5770 T5767 prep in,were
R5016 T5771 T5774 amod CFTRinh172-treated,cells
R5017 T5772 T5774 nummod THP-1,cells
R5018 T5773 T5774 compound reporter,cells
R5019 T5774 T5770 pobj cells,in
R5020 T5775 T5767 punct .,were
R6520 T7430 T7434 nsubjpass Levels,increased
R6521 T7431 T7430 prep of,Levels
R6522 T7432 T7431 pobj IL-1β,of
R6523 T7433 T7434 auxpass are,increased
R6524 T7434 T7434 ROOT increased,increased
R6525 T7435 T7434 prep in,increased
R6526 T7436 T7437 det the,BALF
R6527 T7437 T7435 pobj BALF,in
R6528 T7438 T7437 prep of,BALF
R6529 T7439 T7440 compound CF,patients
R6530 T7440 T7438 pobj patients,of
R6531 T7441 T7434 cc but,increased
R6532 T7442 T7444 det the,source
R6533 T7443 T7444 amod cellular,source
R6534 T7444 T7458 nsubj source,are
R6535 T7445 T7444 prep of,source
R6536 T7446 T7447 det this,cytokine
R6537 T7447 T7445 pobj cytokine,of
R6538 T7448 T7447 cc and,cytokine
R6539 T7449 T7450 poss its,production
R6540 T7450 T7444 conj production,source
R6541 T7451 T7450 prep in,production
R6542 T7452 T7453 det the,context
R6543 T7453 T7451 pobj context,in
R6544 T7454 T7453 prep of,context
R6545 T7455 T7457 amod targeted,activation
R6546 T7456 T7457 amod inflammasome,activation
R6547 T7457 T7454 pobj activation,of
R6618 T7581 T7582 poss its,loss
R6619 T7582 T7578 conj loss,]
R6620 T7583 T7585 auxpass is,associated
R6621 T7584 T7585 advmod frequently,associated
R6622 T7585 T7549 conj associated,expressed
R6623 T7586 T7585 prep with,associated
R6624 T7509 T7511 det a,increase
R6625 T7587 T7590 det an,phenotype
R6626 T7588 T7590 amod augmented,phenotype
R6627 T7589 T7590 amod inflammatory,phenotype
R6628 T7590 T7586 pobj phenotype,with
R6629 T7510 T7511 amod significant,increase
R6630 T7591 T7549 punct .,expressed
R6631 T7592 T7624 prep Despite,shown
R6632 T7593 T7594 poss our,findings
R6633 T7594 T7592 pobj findings,Despite
R6634 T7595 T7594 acl indicating,findings
R6635 T7511 T7508 dobj increase,show
R6636 T7596 T7604 mark that,are
R6637 T7597 T7599 amod bronchial,cells
R6638 T7598 T7599 amod epithelial,cells
R6639 T7599 T7604 nsubj cells,are
R6640 T7512 T7511 prep in,increase
R6641 T7600 T7601 advmod when,grown
R6642 T7601 T7599 advcl grown,cells
R6643 T7602 T7601 prep in,grown
R6644 T7513 T7514 amod caspase-1,activation
R6645 T7603 T7602 pobj vitro,in
R6646 T7514 T7512 pobj activation,in
R6647 T7604 T7595 ccomp are,indicating
R6648 T7605 T7604 acomp unlikely,are
R6649 T7515 T7511 prep in,increase
R6650 T7606 T7609 aux to,involved
R6651 T7607 T7609 auxpass be,involved
R6652 T7608 T7609 advmod significantly,involved
R6653 T7516 T7515 pobj response,in
R6654 T7609 T7605 xcomp involved,unlikely
R6655 T7610 T7609 prep in,involved
R6656 T7611 T7613 det the,production
R6657 T7517 T7516 prep to,response
R6658 T7612 T7613 amod direct,production
R6659 T7613 T7610 pobj production,in
R6660 T7518 T7517 pobj PAO1,to
R6661 T7614 T7613 prep of,production
R6662 T7519 T7518 cc and,PAO1
R6663 T7520 T7518 conj LPS+ATP,PAO1
R6664 T7615 T7614 pobj IL-1β,of
R6665 T7521 T7511 punct ",",increase
R6666 T7616 T7615 punct (,IL-1β
R6667 T7522 T7508 prep in,show
R6668 T7617 T7615 appos Fig.,IL-1β
R6669 T7523 T7522 pobj comparison,in
R6670 T7618 T7617 nummod 2a,Fig.
R6671 T7619 T7615 appos d,IL-1β
R6672 T7620 T7615 punct ),IL-1β
R6673 T7621 T7624 punct ",",shown
R6674 T7622 T7624 nsubj others,shown
R6675 T7623 T7624 aux have,shown
R6676 T7624 T7624 ROOT shown,shown
R6677 T7625 T7626 poss their,capacity
R6678 T7626 T7624 dobj capacity,shown
R6679 T7524 T7523 prep to,comparison
R6680 T7627 T7628 aux to,respond
R6681 T7628 T7626 acl respond,capacity
R6682 T7629 T7628 prep to,respond
R6683 T7525 T7527 amod hematopoeitic,cells
R6684 T7630 T7632 advmod alveolar,IL-1β
R6685 T7631 T7632 amod macrophage-derived,IL-1β
R6686 T7632 T7629 pobj IL-1β,to
R6589 T7562 T7550 cc as,in
R6590 T7499 T7500 neg not,produce
R6591 T7563 T7550 conj in,in
R6592 T7500 T7493 ccomp produce,found
R6593 T7564 T7563 pobj PBMCs,in
R6594 T7565 T7549 prep at,expressed
R6595 T7501 T7502 amod significant,amounts
R6596 T7566 T7568 preconj both,mRNA
R6597 T7567 T7568 det the,mRNA
R6598 T7502 T7500 dobj amounts,produce
R6599 T7568 T7565 pobj mRNA,at
R6600 T7569 T7568 cc and,mRNA
R6601 T7570 T7571 compound protein,level
R6602 T7503 T7502 prep of,amounts
R6603 T7571 T7572 compound level,[
R6604 T7572 T7568 conj [,mRNA
R6605 T7573 T7574 nummod 40,]
R6606 T7504 T7503 pobj IL-1β,of
R6607 T7574 T7568 conj ],mRNA
R6608 T7575 T7574 punct ",",]
R6609 T7576 T7574 conj [,]
R6610 T7505 T7500 cc and,produce
R6611 T7506 T7508 aux do,show
R6612 T7577 T7578 nummod 41,]
R6613 T7507 T7508 neg not,show
R6614 T7578 T7574 conj ],]
R6615 T7579 T7578 punct ",",]
R6616 T7508 T7500 conj show,produce
R6617 T7580 T7578 cc and,]
R6828 T7757 T7753 appos b,controls
R6829 T7737 T7737 ROOT found,found
R6830 T7738 T7746 mark that,increased
R6831 T7739 T7740 amod IL-1β,production
R6832 T7758 T7746 punct ),increased
R6833 T7740 T7746 nsubjpass production,increased
R6834 T7741 T7740 prep in,production
R6835 T7742 T7743 compound CF,PBMCs
R6836 T7759 T7737 punct .,found
R6837 T7743 T7741 pobj PBMCs,in
R6838 T7760 T7761 nsubj This,was
R6839 T7744 T7746 auxpass was,increased
R6840 T7761 T7761 ROOT was,was
R6841 T7745 T7746 neg not,increased
R6842 T7746 T7737 ccomp increased,found
R6843 T7762 T7761 prep in,was
R6844 T7747 T7746 prep upon,increased
R6845 T7748 T7749 amod inflammasome,stimulation
R6846 T7749 T7747 pobj stimulation,upon
R6847 T7750 T7751 mark as,compared
R6848 T7751 T7746 advcl compared,increased
R6849 T7763 T7762 pobj contrast,in
R6850 T7752 T7751 prep to,compared
R6851 T7753 T7752 pobj controls,to
R6852 T7754 T7753 punct (,controls
R6853 T7764 T7763 prep to,contrast
R6687 T7633 T7628 cc and,respond
R6688 T7526 T7527 amod mononuclear,cells
R6689 T7634 T7635 aux to,amplify
R6690 T7635 T7628 conj amplify,respond
R6691 T7636 T7638 det the,response
R6692 T7527 T7524 pobj cells,to
R6693 T7637 T7638 amod inflammatory,response
R6694 T7638 T7635 dobj response,amplify
R6695 T7639 T7635 prep through,amplify
R6696 T7528 T7493 punct .,found
R6697 T7640 T7641 det the,induction
R6698 T7641 T7639 pobj induction,through
R6699 T7529 T7530 amod Hematopoeitically-derived,cells
R6700 T7642 T7641 prep of,induction
R6701 T7643 T7642 pobj chemokines,of
R6702 T7644 T7643 cc and,chemokines
R6703 T7530 T7538 nsubj cells,appear
R6704 T7645 T7643 conj recruitment,chemokines
R6705 T7646 T7645 prep of,recruitment
R6706 T7647 T7649 amod inflammatory,cells
R6707 T7531 T7530 punct ",",cells
R6708 T7648 T7649 compound effector,cells
R6709 T7649 T7646 pobj cells,of
R6710 T7650 T7652 nmod [,]
R6711 T7532 T7533 amod such,as
R6712 T7651 T7652 nummod 42,]
R6713 T7652 T7641 appos ],induction
R6714 T7653 T7624 punct .,shown
R6715 T7654 T7655 det This,interaction
R6716 T7533 T7530 prep as,cells
R6717 T7655 T7657 nsubj interaction,constitute
R6718 T7656 T7657 aux may,constitute
R6719 T7534 T7533 pobj monocytes,as
R6720 T7657 T7657 ROOT constitute,constitute
R6721 T7658 T7660 det a,component
R6722 T7535 T7534 cc and,monocytes
R6723 T7536 T7534 conj macrophages,monocytes
R6724 T7537 T7530 punct ",",cells
R6725 T7659 T7660 amod critical,component
R6726 T7660 T7657 dobj component,constitute
R6727 T7661 T7662 aux to,effective
R6728 T7538 T7538 ROOT appear,appear
R6729 T7662 T7660 acl effective,component
R6730 T7663 T7664 compound host,defense
R6731 T7664 T7662 dobj defense,effective
R6732 T7539 T7540 aux to,be
R6733 T7665 T7662 cc and,effective
R6734 T7666 T7662 conj diminishing,effective
R6735 T7667 T7668 det the,capacity
R6736 T7540 T7538 xcomp be,appear
R6737 T7668 T7666 dobj capacity,diminishing
R6738 T7669 T7668 prep of,capacity
R6739 T7541 T7543 det a,source
R6740 T7670 T7671 compound host,cells
R6741 T7671 T7669 pobj cells,of
R6742 T7672 T7673 aux to,respond
R6743 T7673 T7657 advcl respond,constitute
R6744 T7542 T7543 amod principal,source
R6745 T7674 T7673 prep to,respond
R6746 T7675 T7674 pobj IL-1β,to
R6747 T7676 T7677 aux may,leave
R6748 T7543 T7540 attr source,be
R6749 T7677 T7657 conj leave,constitute
R6750 T7678 T7679 det the,host
R6751 T7679 T7677 dobj host,leave
R6752 T7544 T7543 prep of,source
R6753 T7680 T7677 oprd susceptible,leave
R6754 T7681 T7680 prep to,susceptible
R6755 T7682 T7681 pobj infections,to
R6756 T7545 T7544 pobj IL-1β,of
R6757 T7683 T7677 prep by,leave
R6758 T7684 T7683 pobj pathogens,by
R6759 T7685 T7686 amod such,as
R6760 T7546 T7538 punct .,appear
R6761 T7686 T7684 prep as,pathogens
R6762 T7687 T7688 compound P.,aeruginosa
R6763 T7547 T7549 nsubjpass CFTR,expressed
R6764 T7548 T7549 auxpass is,expressed
R6765 T7688 T7689 compound aeruginosa,[
R6766 T7689 T7686 pobj [,as
R6767 T7690 T7691 nummod 43,]
R6768 T7549 T7549 ROOT expressed,expressed
R6769 T7691 T7689 appos ],[
R6770 T7692 T7657 punct .,constitute
R6771 T7550 T7549 prep in,expressed
R6772 T7693 T7700 advmod Conversely,play
R6773 T7694 T7700 punct ",",play
R6774 T7695 T7700 nsubj overproduction,play
R6775 T7551 T7552 amod alveolar,macrophages
R6776 T7696 T7695 prep of,overproduction
R6777 T7697 T7696 pobj IL-1β,of
R6778 T7698 T7700 aux can,play
R6779 T7552 T7553 compound macrophages,[
R6780 T7699 T7700 advmod also,play
R6781 T7700 T7700 ROOT play,play
R6782 T7701 T7703 det a,role
R6783 T7702 T7703 amod key,role
R6784 T7553 T7555 nmod [,]
R6785 T7703 T7700 dobj role,play
R6786 T7704 T7700 prep in,play
R6787 T7554 T7555 nummod 32,]
R6788 T7705 T7707 amod chronic,responses
R6789 T7706 T7707 amod inflammatory,responses
R6790 T7555 T7550 pobj ],in
R6791 T7707 T7704 pobj responses,in
R6792 T7708 T7707 cc and,responses
R6793 T7709 T7710 compound cause,damage
R6794 T7710 T7707 conj damage,responses
R6795 T7556 T7555 punct ",",]
R6796 T7711 T7710 prep to,damage
R6797 T7712 T7714 det the,parenchyma
R6798 T7713 T7714 compound lung,parenchyma
R6799 T7714 T7711 pobj parenchyma,to
R6800 T7557 T7559 compound [,]
R6801 T7715 T7717 nmod [,]
R6802 T7716 T7717 nummod 44,]
R6803 T7717 T7714 appos ],parenchyma
R6804 T7558 T7559 compound 39,]
R6805 T7718 T7717 punct ",",]
R6806 T7719 T7721 nmod [,]
R6807 T7720 T7721 nummod 45,]
R6808 T7559 T7555 appos ],]
R6809 T7721 T7717 appos ],]
R6810 T7722 T7700 punct .,play
R6811 T7723 T7725 mark Although,hypothesized
R6812 T7560 T7562 advmod as,as
R6813 T7724 T7725 nsubj we,hypothesized
R6814 T7725 T7737 advcl hypothesized,found
R6815 T7726 T7730 mark that,secrete
R6816 T7561 T7562 advmod well,as
R6817 T7727 T7728 compound CF,cells
R6818 T7728 T7730 nsubj cells,secrete
R6819 T7729 T7730 aux would,secrete
R6820 T7756 T7755 nummod 5a,Fig.
R6821 T7730 T7725 ccomp secrete,hypothesized
R6822 T7731 T7732 amod increased,amounts
R6823 T7732 T7730 dobj amounts,secrete
R6824 T7733 T7732 prep of,amounts
R6825 T7734 T7733 pobj IL-1β,of
R6826 T7735 T7737 punct ",",found
R6827 T7736 T7737 nsubj we,found
R6903 T7852 T7853 compound cytokine,production
R6904 T7783 T7782 pobj LPS,to
R6905 T7784 T7786 nmod [,]
R6906 T7785 T7786 nummod 46,]
R6907 T7853 T7849 pobj production,to
R6908 T7854 T7853 prep including,production
R6909 T7786 T7783 appos ],LPS
R6910 T7855 T7857 det an,availability
R6911 T7856 T7857 amod increased,availability
R6912 T7857 T7854 pobj availability,including
R6913 T7787 T7761 punct ",",was
R6914 T7858 T7857 prep of,availability
R6915 T7788 T7793 mark although,accounted
R6916 T7789 T7790 det this,difference
R6917 T7859 T7858 pobj pro-IL-1β,of
R6918 T7860 T7857 prep for,availability
R6919 T7790 T7793 nsubjpass difference,accounted
R6854 T7755 T7753 appos Fig.,controls
R6855 T7765 T7767 det a,study
R6856 T7766 T7767 amod previous,study
R6857 T7767 T7764 pobj study,to
R6858 T7768 T7767 prep from,study
R6859 T7769 T7770 poss our,group
R6860 T7770 T7768 pobj group,from
R6861 T7822 T7824 mark if,were
R6862 T7771 T7770 punct ",",group
R6863 T7823 T7824 expl there,were
R6864 T7824 T7821 advcl were,anticipated
R6865 T7825 T7826 amod basal,levels
R6866 T7772 T7773 nsubj which,showed
R6867 T7826 T7824 attr levels,were
R6868 T7827 T7826 prep of,levels
R6869 T7828 T7829 amod NF-κB,activation
R6870 T7829 T7827 pobj activation,of
R6871 T7773 T7770 relcl showed,group
R6872 T7830 T7810 punct .,increased
R6873 T7831 T7832 nsubj Cells,deficient
R6874 T7832 T7832 ROOT deficient,deficient
R6875 T7833 T7832 prep in,deficient
R6876 T7834 T7833 pobj CFTR,in
R6877 T7835 T7836 auxpass are,thought
R6878 T7774 T7776 amod increased,production
R6879 T7836 T7832 conj thought,deficient
R6880 T7837 T7838 aux to,exhibit
R6881 T7838 T7836 xcomp exhibit,thought
R6882 T7775 T7776 amod IL-1β,production
R6883 T7839 T7842 det an,level
R6884 T7840 T7842 amod increased,level
R6885 T7841 T7842 amod basal,level
R6886 T7776 T7773 dobj production,showed
R6887 T7842 T7838 dobj level,exhibit
R6888 T7843 T7842 prep of,level
R6889 T7844 T7845 amod NF-κB,activity
R6890 T7777 T7776 prep by,production
R6891 T7845 T7843 pobj activity,of
R6892 T7846 T7845 punct ",",activity
R6893 T7847 T7848 nsubj which,leads
R6894 T7778 T7779 compound CF,PBMCs
R6895 T7779 T7777 pobj PBMCs,by
R6896 T7780 T7773 prep in,showed
R6897 T7848 T7842 relcl leads,level
R6898 T7781 T7780 pobj response,in
R6899 T7849 T7848 prep to,leads
R6900 T7850 T7853 amod increased,production
R6901 T7851 T7853 amod pro-inflammatory,production
R6902 T7782 T7781 prep to,response
R7088 T7987 T7988 aux to,produce
R7089 T8058 T8059 det the,study
R7090 T7988 T7986 acl produce,capacity
R7091 T7989 T7988 dobj IL-1β,produce
R7092 T8059 T8057 pobj study,with
R7093 T7990 T7982 cc and,had
R7094 T7991 T7982 conj lacked,had
R7095 T7992 T7993 det the,ability
R6920 T7861 T7860 pobj cleavage,for
R6921 T7862 T7861 cc and,cleavage
R6922 T7863 T7861 conj secretion,cleavage
R6923 T7864 T7832 punct .,deficient
R6924 T7865 T7866 det This,amplification
R6925 T7866 T7871 nsubjpass amplification,shown
R6926 T7867 T7866 prep of,amplification
R6927 T7791 T7793 aux may,accounted
R6928 T7868 T7869 amod IL-1β,secretion
R6929 T7869 T7867 pobj secretion,of
R6930 T7870 T7871 auxpass was,shown
R6931 T7792 T7793 auxpass be,accounted
R6932 T7871 T7871 ROOT shown,shown
R6933 T7872 T7871 agent by,shown
R6934 T7793 T7761 advcl accounted,was
R6935 T7873 T7872 pcomp priming,by
R6936 T7874 T7875 nummod THP-1,monocytes
R6937 T7875 T7873 dobj monocytes,priming
R6938 T7876 T7875 cc and,monocytes
R6939 T7794 T7793 prep for,accounted
R6940 T7877 T7875 conj PBMCs,monocytes
R6941 T7878 T7873 prep with,priming
R6942 T7795 T7793 agent by,accounted
R6943 T7879 T7881 amod heat-killed,aeruginosa
R6944 T7880 T7881 compound P.,aeruginosa
R6945 T7881 T7878 pobj aeruginosa,with
R6946 T7796 T7797 amod technical,issues
R6947 T7882 T7881 cc or,aeruginosa
R6948 T7883 T7881 conj LPS,aeruginosa
R6949 T7884 T7873 advmod prior,priming
R6950 T7885 T7884 prep to,prior
R6951 T7797 T7795 pobj issues,by
R6952 T7886 T7885 pobj stimulation,to
R6953 T7887 T7873 prep with,priming
R6954 T7798 T7793 prep in,accounted
R6955 T7888 T7890 amod live,aeruginosa
R6956 T7889 T7890 compound P.,aeruginosa
R6957 T7890 T7887 pobj aeruginosa,with
R6958 T7799 T7800 compound stimulation,time
R6959 T7891 T7871 punct .,shown
R6960 T7892 T7896 det This,secretion
R6961 T7893 T7894 advmod dramatically,increased
R6962 T7800 T7798 pobj time,in
R6963 T7894 T7896 amod increased,secretion
R6964 T7895 T7896 amod IL-1β,secretion
R6965 T7896 T7896 ROOT secretion,secretion
R6966 T7801 T7800 cc and,time
R6967 T7897 T7896 prep over,secretion
R6968 T7898 T7897 pobj stimulation,over
R6969 T7899 T7896 prep with,secretion
R6970 T7900 T7902 amod live,aeruginosa
R6971 T7802 T7800 conj dose,time
R6972 T7901 T7902 compound P.,aeruginosa
R6973 T7902 T7899 pobj aeruginosa,with
R6974 T7903 T7896 prep without,secretion
R6975 T7904 T7903 pcomp priming,without
R6976 T7905 T7906 punct (,Fig.
R6977 T7906 T7896 appos Fig.,secretion
R6978 T7803 T7761 punct .,was
R6979 T7907 T7906 nummod 6c,Fig.
R6980 T7908 T7906 punct ),Fig.
R6981 T7909 T7896 punct .,secretion
R6982 T7804 T7810 advmod Moreover,increased
R6983 T7910 T7923 advmod Similarly,be
R6984 T7911 T7923 punct ",",be
R6985 T7912 T7915 mark if,expressed
R6986 T7805 T7810 punct ",",increased
R6987 T7913 T7914 compound CF,PBMCs
R6988 T7914 T7915 nsubj PBMCs,expressed
R6989 T7915 T7923 advcl expressed,be
R6990 T7806 T7807 amod IL-1β,production
R6991 T7916 T7919 amod increased,activity
R6992 T7917 T7919 amod basal,activity
R6993 T7918 T7919 compound NF-κB,activity
R6994 T7807 T7810 nsubjpass production,increased
R6995 T7919 T7915 dobj activity,expressed
R6996 T7920 T7923 punct ",",be
R6997 T7921 T7923 expl there,be
R6998 T7922 T7923 aux would,be
R6999 T7808 T7810 auxpass was,increased
R7000 T7923 T7923 ROOT be,be
R7001 T7924 T7925 det an,increase
R7002 T7925 T7923 attr increase,be
R7003 T7809 T7810 neg not,increased
R7004 T7926 T7925 prep in,increase
R7005 T7927 T7928 amod IL-1β,secretion
R7006 T7810 T7810 ROOT increased,increased
R7007 T7928 T7926 pobj secretion,in
R7008 T7929 T7925 prep in,increase
R7009 T7930 T7931 det the,absence
R7010 T7811 T7810 prep in,increased
R7011 T7931 T7929 pobj absence,in
R7012 T7812 T7813 compound CF,PBMCs
R7013 T7932 T7931 prep of,absence
R7014 T7933 T7934 compound LPS,priming
R7015 T7934 T7932 pobj priming,of
R7016 T7813 T7811 pobj PBMCs,in
R7017 T7935 T7923 punct .,be
R7018 T7936 T7943 advmod However,observed
R7019 T7937 T7943 punct ",",observed
R7020 T7814 T7810 prep with,increased
R7021 T7938 T7939 det no,increase
R7022 T7939 T7943 nsubjpass increase,observed
R7023 T7940 T7939 prep in,increase
R7024 T7815 T7816 amod inflammasome,stimulation
R7025 T7941 T7940 pobj IL-1β,in
R7026 T7942 T7943 auxpass was,observed
R7027 T7816 T7814 pobj stimulation,with
R7028 T7943 T7943 ROOT observed,observed
R7029 T7944 T7943 prep under,observed
R7030 T7945 T7944 pobj basal,under
R7031 T7946 T7945 cc or,basal
R7032 T7817 T7816 advmod alone,stimulation
R7033 T7947 T7948 amod primed,conditions
R7034 T7948 T7945 conj conditions,basal
R7035 T7818 T7821 mark as,anticipated
R7036 T7949 T7943 punct .,observed
R7037 T7819 T7821 aux would,anticipated
R7038 T7820 T7821 auxpass be,anticipated
R7039 T7821 T7810 advcl anticipated,increased
R7040 T7950 T7957 nsubj Studies,been
R7041 T8047 T8046 pobj monocytes,of
R7042 T7951 T7950 acl investigating,Studies
R7043 T7952 T7953 det the,production
R7044 T7953 T7951 dobj production,investigating
R7045 T7954 T7953 prep of,production
R7046 T8048 T8045 prep into,differentiation
R7047 T7955 T7954 pobj IL-1β,of
R7048 T7956 T7957 aux have,been
R7049 T7957 T7957 ROOT been,been
R7050 T8049 T8048 pobj macrophages,into
R7051 T7958 T7959 advmod somewhat,inconsistent
R7052 T7959 T7957 acomp inconsistent,been
R7053 T7960 T7957 punct .,been
R7054 T8050 T8052 aux may,account
R7055 T7961 T7962 det A,study
R7056 T7962 T7970 nsubj study,provided
R7057 T7963 T7962 prep by,study
R7058 T8051 T8052 advmod partly,account
R7059 T7964 T7963 pobj Reininger,by
R7060 T8052 T8052 ROOT account,account
R7061 T7965 T7967 nmod et,[
R7062 T7966 T7967 compound al.,[
R7063 T7967 T7969 nmod [,]
R7064 T7968 T7969 nummod 43,]
R7065 T8053 T8052 prep for,account
R7066 T7969 T7970 nsubj ],provided
R7067 T7970 T7970 ROOT provided,provided
R7068 T7971 T7970 dobj evidence,provided
R7069 T8054 T8055 det the,differences
R7070 T7972 T7982 mark that,had
R7071 T7973 T7976 amod human,cells
R7072 T7974 T7976 amod bronchial,cells
R7073 T8055 T8053 pobj differences,for
R7074 T7975 T7976 amod epithelial,cells
R7075 T7976 T7982 nsubj cells,had
R7076 T7977 T7976 acl possessing,cells
R7077 T7978 T7981 det the,mutation
R7096 T8060 T8056 agent by,observed
R7097 T7993 T7991 dobj ability,lacked
R7098 T7994 T7995 aux to,induce
R7099 T7995 T7993 acl induce,ability
R7100 T7996 T7999 det an,activation
R7101 T8061 T8060 pobj Kotrange,by
R7102 T7997 T7999 amod early,activation
R7103 T7998 T7999 compound NF-κB,activation
R7104 T7999 T7995 dobj activation,induce
R7105 T8062 T8052 punct .,account
R7106 T8000 T7995 prep in,induce
R7107 T8001 T8000 pobj response,in
R7108 T8002 T8001 prep to,response
R7109 T8063 T8065 amod Inflammasome-mediated,production
R7110 T8003 T8004 compound P.,aeruginosa
R7111 T8004 T8002 pobj aeruginosa,to
R7112 T8005 T7970 punct .,provided
R7113 T8064 T8065 compound IL-1β,production
R7114 T8006 T8017 advmod Conversely,found
R7115 T8007 T8017 punct ",",found
R7116 T8065 T8071 nsubj production,differ
R7117 T8008 T8009 det a,study
R7118 T8009 T8017 nsubj study,found
R7119 T8010 T8009 prep by,study
R7120 T8011 T8010 pobj Kotrange,by
R7121 T8066 T8065 prep by,production
R7122 T8012 T8013 compound et,al.
R7123 T8013 T8014 compound al.,[
R7124 T8067 T8066 pobj monocytes,by
R7125 T8014 T8009 appos [,study
R7126 T8015 T8016 nummod 31,]
R7127 T8016 T8017 nsubj ],found
R7128 T8017 T8017 ROOT found,found
R7129 T8018 T8025 mark that,produced
R7130 T8019 T8022 amod murine,macrophages
R7131 T8068 T8067 cc and,monocytes
R7132 T8020 T8022 nmod bone,macrophages
R7133 T8069 T8067 conj PBMCs,monocytes
R7134 T8070 T8071 aux does,differ
R7135 T8021 T8022 amod marrow-derived,macrophages
R7136 T8022 T8025 nsubj macrophages,produced
R7137 T8071 T8071 ROOT differ,differ
R7138 T8023 T8022 acl expressing,macrophages
R7139 T8072 T8071 prep from,differ
R7140 T8024 T8023 dobj ΔF508-CFTR,expressing
R7141 T8073 T8074 compound macrophages,[
R7142 T8074 T8072 pobj [,from
R7143 T8075 T8076 nummod 47,]
R7144 T8025 T8017 ccomp produced,found
R7145 T8076 T8074 conj ],[
R7146 T8026 T8027 amod increased,amounts
R7147 T8027 T8025 dobj amounts,produced
R7148 T8028 T8027 prep of,amounts
R7292 T8116 T8114 dobj ],[
R7293 T8230 T8205 punct .,revealed
R7294 T8231 T8245 prep In,increases
R7295 T8232 T8231 pobj contrast,In
R7296 T8117 T8108 cc and,express
R7078 T7979 T7981 nmod ΔF508,mutation
R7079 T7980 T7981 amod CFTR,mutation
R7080 T8056 T8055 acl observed,differences
R7081 T7981 T7977 dobj mutation,possessing
R7082 T7982 T7971 acl had,evidence
R7083 T7983 T7986 det a,capacity
R7084 T7984 T7985 advmod slightly,reduced
R7085 T8057 T8056 prep with,observed
R7086 T7985 T7986 amod reduced,capacity
R7087 T7986 T7982 dobj capacity,had
R7149 T8077 T8076 punct ",",]
R7150 T8029 T8028 pobj IL-1β,of
R7151 T8030 T8031 advmod when,compared
R7152 T8078 T8080 nmod [,]
R7153 T8031 T8025 advcl compared,produced
R7154 T8032 T8031 prep to,compared
R7155 T8033 T8032 pobj macrophages,to
R7156 T8079 T8080 nummod 48,]
R7157 T8034 T8033 acl expressing,macrophages
R7158 T8035 T8036 amod normal,CFTR
R7159 T8036 T8034 dobj CFTR,expressing
R7160 T8037 T8034 prep in,expressing
R7161 T8038 T8037 pobj response,in
R7162 T8039 T8038 prep to,response
R7163 T8080 T8076 conj ],]
R7164 T8040 T8042 compound Burkholderia,K56-2
R7165 T8081 T8080 punct ",",]
R7166 T8041 T8042 compound cenocepacia,K56-2
R7167 T8082 T8080 conj [,]
R7168 T8042 T8039 pobj K56-2,to
R7169 T8043 T8017 punct .,found
R7170 T8044 T8045 det The,differentiation
R7171 T8083 T8084 nummod 49,]
R7172 T8045 T8052 nsubj differentiation,account
R7173 T8046 T8045 prep of,differentiation
R7174 T8084 T8080 conj ],]
R7175 T8085 T8084 cc and,]
R7176 T8086 T8084 conj macrophages,]
R7177 T8087 T8088 auxpass are,found
R7178 T8144 T8144 ROOT contribute,contribute
R7179 T8088 T8071 conj found,differ
R7180 T8145 T8144 prep to,contribute
R7181 T8146 T8147 compound IL-1β,production
R7182 T8147 T8145 pobj production,to
R7183 T8089 T8090 aux to,have
R7184 T8148 T8144 punct .,contribute
R7185 T8149 T8157 prep For,increased
R7186 T8150 T8149 pobj example,For
R7187 T8090 T8088 xcomp have,found
R7188 T8151 T8157 punct ",",increased
R7189 T8152 T8153 compound neutrophil,counts
R7190 T8153 T8157 nsubjpass counts,increased
R7191 T8091 T8092 amod higher,expression
R7192 T8154 T8157 aux can,increased
R7193 T8155 T8157 auxpass be,increased
R7194 T8156 T8157 advmod significantly,increased
R7195 T8092 T8090 dobj expression,have
R7196 T8157 T8157 ROOT increased,increased
R7197 T8158 T8157 prep in,increased
R7198 T8093 T8092 prep of,expression
R7199 T8159 T8160 det the,lungs
R7200 T8094 T8093 pobj CFTR,of
R7201 T8160 T8158 pobj lungs,in
R7202 T8161 T8160 prep of,lungs
R7203 T8162 T8163 compound CF,patients
R7204 T8095 T8092 prep over,expression
R7205 T8163 T8161 pobj patients,of
R7206 T8164 T8166 nmod [,]
R7207 T8165 T8166 nummod 52,]
R7208 T8096 T8097 compound monocytes,[
R7209 T8166 T8157 npadvmod ],increased
R7210 T8167 T8166 punct ",",]
R7211 T8097 T8095 pobj [,over
R7212 T8168 T8166 appos [,]
R7213 T8169 T8170 nummod 53,]
R7214 T8170 T8166 conj ],]
R7215 T8098 T8099 nummod 50,]
R7216 T8171 T8170 punct ",",]
R7217 T8172 T8170 conj [,]
R7218 T8099 T8097 appos ],[
R7219 T8173 T8174 nummod 54,]
R7220 T8174 T8170 conj ],]
R7221 T8175 T8157 cc and,increased
R7222 T8100 T8071 punct .,differ
R7223 T8176 T8177 aux may,produce
R7224 T8177 T8157 conj produce,increased
R7225 T8101 T8125 advmod However,are
R7226 T8178 T8179 amod mature,IL-1β
R7227 T8179 T8177 dobj IL-1β,produce
R7228 T8180 T8177 prep through,produce
R7229 T8181 T8183 amod caspase-1,mechanisms
R7230 T8182 T8183 amod independent,mechanisms
R7231 T8183 T8180 pobj mechanisms,through
R7232 T8102 T8125 punct ",",are
R7233 T8184 T8186 nmod [,]
R7234 T8185 T8186 nummod 55,]
R7235 T8186 T8183 appos ],mechanisms
R7236 T8103 T8108 mark as,express
R7237 T8187 T8157 punct .,increased
R7238 T8188 T8189 det The,role
R7239 T8189 T8199 nsubj role,is
R7240 T8104 T8108 nsubj monocytes,express
R7241 T8190 T8189 prep of,role
R7242 T8191 T8192 amod NF-κB,activation
R7243 T8105 T8104 cc and,monocytes
R7244 T8192 T8190 pobj activation,of
R7245 T8193 T8192 prep in,activation
R7246 T8194 T8195 amod inflammasome,activation
R7247 T8195 T8193 pobj activation,in
R7248 T8106 T8107 amod other,PBMCs
R7249 T8196 T8195 cc and,activation
R7250 T8197 T8198 amod IL-1β,secretion
R7251 T8198 T8195 conj secretion,activation
R7252 T8107 T8104 conj PBMCs,monocytes
R7253 T8199 T8199 ROOT is,is
R7254 T8200 T8199 neg not,is
R7255 T8108 T8125 advcl express,are
R7256 T8201 T8199 acomp straightforward,is
R7257 T8202 T8199 punct .,is
R7258 T8203 T8205 nsubj Studies,revealed
R7259 T8204 T8205 aux have,revealed
R7260 T8109 T8110 compound CFTR,[
R7261 T8205 T8205 ROOT revealed,revealed
R7262 T8206 T8208 det an,role
R7263 T8207 T8208 amod essential,role
R7264 T8110 T8112 nmod [,]
R7265 T8208 T8205 dobj role,revealed
R7266 T8209 T8208 prep for,role
R7267 T8210 T8211 amod NF-κB,activation
R7268 T8211 T8209 pobj activation,for
R7269 T8111 T8112 nummod 50,]
R7270 T8212 T8211 prep in,activation
R7271 T8213 T8214 det the,production
R7272 T8214 T8212 pobj production,in
R7273 T8112 T8108 dobj ],express
R7274 T8215 T8214 prep of,production
R7275 T8216 T8219 amod pro-IL-1β,components
R7276 T8113 T8112 punct ",",]
R7277 T8217 T8216 cc and,pro-IL-1β
R7278 T8218 T8216 conj inflammasome,pro-IL-1β
R7279 T8219 T8215 pobj components,of
R7280 T8220 T8221 amod such,as
R7281 T8221 T8219 prep as,components
R7282 T8222 T8223 compound NLRP3,[
R7283 T8114 T8112 conj [,]
R7284 T8223 T8221 pobj [,as
R7285 T8224 T8225 nummod 22,]
R7286 T8225 T8223 conj ],[
R7287 T8226 T8225 punct ",",]
R7288 T8115 T8116 nummod 51,]
R7289 T8227 T8225 conj [,]
R7290 T8228 T8229 nummod 23,]
R7291 T8229 T8225 conj ],]
R7323 T8249 T8250 compound murine,macrophages
R7324 T8250 T8248 pobj macrophages,in
R7325 T8128 T8129 aux to,examine
R7326 T8251 T8253 nmod [,]
R7327 T8252 T8253 nummod 56,]
R7297 T8233 T8245 punct ",",increases
R7298 T8234 T8245 nsubj deletion,increases
R7299 T8235 T8234 prep of,deletion
R7300 T8118 T8108 conj produce,express
R7301 T8236 T8235 pobj IKKβ,of
R7302 T8237 T8236 punct ",",IKKβ
R7303 T8119 T8120 amod large,amounts
R7304 T8238 T8239 det a,kinase
R7305 T8120 T8118 dobj amounts,produce
R7306 T8239 T8236 appos kinase,IKKβ
R7307 T8240 T8239 amod essential,kinase
R7308 T8121 T8120 prep of,amounts
R7309 T8122 T8121 pobj IL-1β,of
R7310 T8123 T8125 punct ",",are
R7311 T8124 T8125 nsubj they,are
R7312 T8241 T8240 prep in,essential
R7313 T8242 T8243 amod NF-κB,activation
R7314 T8243 T8241 pobj activation,in
R7315 T8125 T8125 ROOT are,are
R7316 T8244 T8236 punct ",",IKKβ
R7317 T8245 T8245 ROOT increases,increases
R7318 T8246 T8247 amod IL-1β,secretion
R7319 T8126 T8127 amod adequate,models
R7320 T8247 T8245 dobj secretion,increases
R7321 T8248 T8247 prep in,secretion
R7322 T8127 T8125 attr models,are
R7352 T8272 T8273 det this,uncertainty
R7424 T8325 T8323 pobj treatment,with
R7425 T8347 T8348 compound Fig.,6d
R7426 T8326 T8314 punct .,were
R7427 T8327 T8328 amod Subsequent,treatment
R7428 T8348 T8349 nummod 6d,h
R7429 T8328 T8340 nsubj treatment,inhibited
R7430 T8329 T8328 prep of,treatment
R7431 T8330 T8331 nummod THP-1,cells
R7432 T8331 T8329 pobj cells,of
R7433 T8332 T8331 cc and,cells
R7434 T8333 T8331 conj PBMCs,cells
R7435 T8349 T8345 appos h,secretion
R7436 T8334 T8328 prep with,treatment
R7437 T8335 T8338 det the,Bay11-7082
R7438 T8336 T8338 amod NF-κB,Bay11-7082
R7439 T8350 T8349 punct ),h
R7440 T8351 T8340 punct .,inhibited
R7441 T8337 T8338 compound inhibitor,Bay11-7082
R7442 T8352 T8359 advmod Therefore,observed
R7443 T8353 T8359 punct ",",observed
R7444 T8354 T8355 det the,IL-1β
R7445 T8355 T8358 nmod IL-1β,responses
R7446 T8356 T8355 cc and,IL-1β
R7447 T8435 T8432 pobj response,of
R7448 T8357 T8355 conj IL-8,IL-1β
R7449 T8436 T8435 acl generated,response
R7450 T8437 T8436 agent by,generated
R7451 T8358 T8359 nsubj responses,observed
R7452 T8438 T8437 pobj live,by
R7453 T8439 T8440 mark as,opposed
R7454 T8359 T8359 ROOT observed,observed
R7455 T8440 T8438 advcl opposed,live
R7456 T8441 T8440 prep to,opposed
R7328 T8253 T8245 dobj ],increases
R7329 T8254 T8253 punct ",",]
R7330 T8255 T8257 nmod [,]
R7331 T8129 T8127 relcl examine,models
R7332 T8256 T8257 nummod 57,]
R7333 T8257 T8253 conj ],]
R7334 T8258 T8245 cc and,increases
R7335 T8259 T8245 conj demonstrates,increases
R7336 T8130 T8131 det the,effects
R7337 T8260 T8262 det a,role
R7338 T8261 T8262 amod dual,role
R7339 T8262 T8259 dobj role,demonstrates
R7340 T8131 T8129 dobj effects,examine
R7341 T8263 T8262 prep for,role
R7342 T8264 T8263 pobj NF-κB,for
R7343 T8265 T8264 prep in,NF-κB
R7344 T8266 T8265 pobj regulation,in
R7345 T8132 T8131 prep of,effects
R7346 T8267 T8266 prep of,regulation
R7347 T8268 T8267 pobj IL-1β,of
R7348 T8269 T8245 punct .,increases
R7349 T8133 T8134 amod CFTR,function
R7350 T8270 T8271 aux To,address
R7351 T8271 T8279 advcl address,quantified
R7353 T8273 T8271 dobj uncertainty,address
R7354 T8134 T8132 pobj function,of
R7355 T8274 T8273 prep in,uncertainty
R7356 T8275 T8276 poss our,experiments
R7357 T8276 T8274 pobj experiments,in
R7358 T8135 T8131 prep on,effects
R7359 T8277 T8279 nsubj we,quantified
R7360 T8278 T8279 advmod also,quantified
R7361 T8136 T8137 compound IL-1β,production
R7362 T8279 T8279 ROOT quantified,quantified
R7363 T8280 T8279 dobj IL-8,quantified
R7364 T8281 T8280 punct ",",IL-8
R7365 T8137 T8135 pobj production,on
R7366 T8282 T8285 det an,cytokine
R7367 T8283 T8285 amod important,cytokine
R7368 T8284 T8285 compound CF,cytokine
R7369 T8285 T8280 appos cytokine,IL-8
R7370 T8138 T8125 punct .,are
R7371 T8286 T8285 cc and,cytokine
R7372 T8287 T8285 conj marker,cytokine
R7373 T8288 T8287 prep of,marker
R7374 T8139 T8141 amod Other,cells
R7375 T8289 T8290 amod NF-κB,activation
R7376 T8290 T8288 pobj activation,of
R7377 T8291 T8280 conj [,IL-8
R7378 T8292 T8293 nummod 58,]
R7379 T8293 T8280 conj ],IL-8
R7380 T8294 T8293 punct ",",]
R7381 T8140 T8141 amod hematopoeitic,cells
R7382 T8295 T8279 cc and,quantified
R7383 T8296 T8279 conj found,quantified
R7384 T8297 T8298 det no,differences
R7385 T8141 T8144 nsubj cells,contribute
R7386 T8298 T8296 dobj differences,found
R7387 T8299 T8298 prep between,differences
R7388 T8300 T8303 nmod CF,subjects
R7389 T8301 T8300 cc and,CF
R7390 T8142 T8144 aux may,contribute
R7391 T8302 T8300 conj control,CF
R7392 T8303 T8299 pobj subjects,between
R7393 T8143 T8144 advmod also,contribute
R7394 T8304 T8279 punct .,quantified
R7395 T8305 T8314 advmod Similarly,were
R7396 T8306 T8314 punct ",",were
R7397 T8338 T8334 pobj Bay11-7082,with
R7398 T8339 T8340 advmod significantly,inhibited
R7399 T8307 T8314 nsubj levels,were
R7400 T8308 T8307 prep of,levels
R7401 T8309 T8310 amod intracellular,pro-IL-1β
R7402 T8340 T8340 ROOT inhibited,inhibited
R7403 T8310 T8308 pobj pro-IL-1β,of
R7404 T8311 T8307 prep in,levels
R7405 T8312 T8313 nummod THP-1,cells
R7406 T8341 T8342 preconj both,IL-1β
R7407 T8313 T8311 pobj cells,in
R7408 T8314 T8314 ROOT were,were
R7409 T8342 T8345 amod IL-1β,secretion
R7410 T8315 T8314 acomp dependent,were
R7411 T8316 T8315 prep on,dependent
R7412 T8317 T8318 amod NF-κB,activity
R7413 T8343 T8342 cc and,IL-1β
R7414 T8318 T8316 pobj activity,on
R7415 T8344 T8342 conj IL-8,IL-1β
R7416 T8319 T8314 cc and,were
R7417 T8320 T8322 aux did,increase
R7418 T8321 T8322 neg not,increase
R7419 T8345 T8340 dobj secretion,inhibited
R7420 T8322 T8314 conj increase,were
R7421 T8346 T8349 punct (,h
R7422 T8323 T8322 prep with,increase
R7423 T8324 T8325 amod CFTRinh172,treatment
R7479 T8459 T8458 cc and,disease
R7480 T8460 T8458 conj issues,disease
R7481 T8461 T8460 acl related,issues
R7482 T8365 T8363 pobj activation,upon
R7483 T8462 T8461 prep to,related
R7484 T8463 T8464 det the,hypermutability
R7485 T8464 T8462 pobj hypermutability,to
R7486 T8366 T8359 punct .,observed
R7487 T8465 T8464 prep of,hypermutability
R7488 T8466 T8467 compound P.,aeruginosa
R7489 T8467 T8465 pobj aeruginosa,of
R7490 T8468 T8461 prep during,related
R7491 T8367 T8376 csubj Priming,is
R7492 T8469 T8470 det the,evolution
R7493 T8470 T8468 pobj evolution,during
R7494 T8471 T8470 prep of,evolution
R7495 T8368 T8367 prep with,Priming
R7496 T8472 T8473 amod chronic,infection
R7497 T8473 T8471 pobj infection,of
R7498 T8369 T8371 amod heat-killed,aeruginosa
R7499 T8474 T8453 punct .,include
R7500 T8475 T8486 mark Although,reflect
R7501 T8370 T8371 compound P.,aeruginosa
R7502 T8476 T8477 det the,responses
R7503 T8371 T8368 pobj aeruginosa,with
R7504 T8477 T8486 nsubj responses,reflect
R7505 T8478 T8477 acl measured,responses
R7506 T8372 T8371 punct ",",aeruginosa
R7507 T8479 T8478 prep in,measured
R7508 T8480 T8482 amod peripheral,cells
R7509 T8481 T8482 compound blood,cells
R7510 T8373 T8371 prep like,aeruginosa
R7511 T8482 T8479 pobj cells,in
R7512 T8483 T8486 aux may,reflect
R7513 T8484 T8486 neg not,reflect
R7514 T8374 T8373 pobj LPS,like
R7515 T8485 T8486 advmod completely,reflect
R7516 T8486 T8496 advcl reflect,have
R7517 T8375 T8376 punct ",",is
R7518 T8487 T8488 det the,responses
R7519 T8488 T8486 dobj responses,reflect
R7520 T8489 T8488 acl occurring,responses
R7521 T8376 T8376 ROOT is,is
R7522 T8490 T8489 prep in,occurring
R7523 T8491 T8493 det the,lung
R7524 T8492 T8493 compound CF,lung
R7525 T8493 T8490 pobj lung,in
R7526 T8377 T8376 acomp unable,is
R7527 T8494 T8496 punct ",",have
R7528 T8495 T8496 nsubj PBMCs,have
R7529 T8496 T8496 ROOT have,have
R7530 T8497 T8498 det a,number
R7531 T8498 T8496 dobj number,have
R7532 T8499 T8498 prep of,number
R7533 T8378 T8379 aux to,induce
R7534 T8500 T8501 amod useful,advantages
R7535 T8501 T8499 pobj advantages,of
R7536 T8502 T8498 punct :,number
R7537 T8503 T8498 punct (,number
R7538 T8379 T8377 xcomp induce,unable
R7539 T8504 T8498 appos i,number
R7540 T8505 T8498 punct ),number
R7541 T8506 T8507 nsubj PBMCs,are
R7457 T8442 T8443 amod dead,bacteria
R7458 T8360 T8359 ccomp were,observed
R7459 T8443 T8441 pobj bacteria,to
R7460 T8444 T8446 nmod [,]
R7461 T8445 T8446 nummod 59,]
R7462 T8446 T8443 appos ],bacteria
R7463 T8361 T8362 advmod both,dependent
R7464 T8447 T8424 punct .,be
R7465 T8448 T8449 amod Potential,shortcomings
R7466 T8449 T8453 nsubj shortcomings,include
R7467 T8362 T8360 amod dependent,were
R7468 T8450 T8449 prep of,shortcomings
R7469 T8451 T8452 det these,experiments
R7470 T8452 T8450 pobj experiments,of
R7471 T8363 T8362 prep upon,dependent
R7472 T8453 T8453 ROOT include,include
R7473 T8454 T8455 poss its,translatability
R7474 T8455 T8453 dobj translatability,include
R7475 T8364 T8365 amod NF-κB,activation
R7476 T8456 T8455 prep to,translatability
R7477 T8457 T8458 compound lung,disease
R7478 T8458 T8456 pobj disease,to
R7629 T8569 T8566 pobj infection,of
R7630 T8570 T8569 cc and,infection
R7631 T8571 T8572 amod genotypic,changes
R7632 T8572 T8569 conj changes,infection
R7633 T8573 T8572 prep in,changes
R7634 T8405 T8401 punct ),activation
R7635 T8574 T8575 compound P.,aeruginosa
R7636 T8575 T8573 pobj aeruginosa,in
R7637 T8576 T8578 mark as,progresses
R7638 T8577 T8578 nsubj infection,progresses
R7639 T8406 T8401 cc and,activation
R7640 T8578 T8562 advcl progresses,given
R7641 T8579 T8562 punct .,given
R7642 T8580 T8581 compound P.,aeruginosa
R7643 T8407 T8408 amod IL-8,secretion
R7644 T8581 T8582 nsubj aeruginosa,mediates
R7645 T8582 T8582 ROOT mediates,mediates
R7646 T8583 T8584 amod inflammasome,activation
R7647 T8408 T8401 conj secretion,activation
R7648 T8584 T8582 dobj activation,mediates
R7649 T8585 T8584 prep through,activation
R7650 T8586 T8590 poss its,system
R7651 T8409 T8410 punct (,Fig.
R7652 T8587 T8590 compound type,system
R7653 T8588 T8590 nmod III,system
R7654 T8589 T8590 compound secretion,system
R7655 T8410 T8401 appos Fig.,activation
R7656 T8590 T8585 pobj system,through
R7657 T8591 T8590 punct (,system
R7658 T8592 T8590 appos T3SS,system
R7659 T8411 T8410 nummod 6b,Fig.
R7660 T8593 T8590 punct ),system
R7661 T8594 T8584 cc and,activation
R7662 T8595 T8600 det the,]
R7663 T8412 T8410 punct ),Fig.
R7664 T8596 T8598 nmod NLRC4,[
R7665 T8597 T8598 compound inflammasome,[
R7666 T8598 T8600 nmod [,]
R7667 T8413 T8401 prep than,activation
R7668 T8599 T8600 nummod 19,]
R7669 T8600 T8584 conj ],activation
R7542 T8380 T8383 det a,response
R7543 T8507 T8507 ROOT are,are
R7544 T8508 T8507 neg not,are
R7545 T8509 T8507 acomp subject,are
R7546 T8381 T8383 amod strong,response
R7547 T8510 T8509 prep to,subject
R7548 T8511 T8510 pobj alterations,to
R7549 T8512 T8514 nsubj that,emerge
R7550 T8382 T8383 amod IL-1β,response
R7551 T8513 T8514 aux may,emerge
R7552 T8514 T8511 relcl emerge,alterations
R7553 T8515 T8514 prep from,emerge
R7554 T8383 T8379 dobj response,induce
R7555 T8516 T8518 amod long-term,culture
R7556 T8517 T8518 compound cell,culture
R7557 T8518 T8515 pobj culture,from
R7558 T8519 T8514 punct ",",emerge
R7559 T8384 T8385 mark as,compared
R7560 T8520 T8514 advcl cloning,emerge
R7561 T8521 T8520 cc and,cloning
R7562 T8385 T8376 advcl compared,is
R7563 T8522 T8520 conj immortalization,cloning
R7564 T8523 T8522 punct ",",immortalization
R7565 T8524 T8522 cc and,immortalization
R7566 T8386 T8387 aux to,live
R7567 T8525 T8526 punct (,ii
R7568 T8387 T8385 xcomp live,compared
R7569 T8526 T8522 conj ii,immortalization
R7570 T8527 T8522 punct ),immortalization
R7571 T8388 T8389 compound P.,aeruginosa
R7572 T8528 T8529 nsubj PBMCs,express
R7573 T8529 T8507 conj express,are
R7574 T8530 T8532 det a,repertoire
R7575 T8389 T8387 dobj aeruginosa,live
R7576 T8531 T8532 amod large,repertoire
R7577 T8390 T8389 punct (,aeruginosa
R7578 T8391 T8389 appos Fig.,aeruginosa
R7579 T8532 T8529 dobj repertoire,express
R7580 T8533 T8532 prep of,repertoire
R7581 T8534 T8536 amod innate,receptors
R7582 T8535 T8536 amod immune,receptors
R7583 T8392 T8391 nummod 6c,Fig.
R7584 T8536 T8533 pobj receptors,of
R7585 T8537 T8529 cc and,express
R7586 T8538 T8529 conj secrete,express
R7587 T8393 T8389 punct ),aeruginosa
R7588 T8539 T8541 det a,array
R7589 T8540 T8541 amod broad,array
R7590 T8541 T8538 dobj array,secrete
R7591 T8394 T8376 punct ",",is
R7592 T8542 T8541 prep of,array
R7593 T8543 T8542 pobj cytokines,of
R7594 T8544 T8543 cc and,cytokines
R7595 T8395 T8376 cc but,is
R7596 T8545 T8543 conj chemokines,cytokines
R7597 T8546 T8541 acl allowing,array
R7598 T8547 T8548 amod comprehensive,analysis
R7599 T8396 T8376 conj generated,is
R7600 T8548 T8546 dobj analysis,allowing
R7601 T8549 T8548 prep of,analysis
R7602 T8550 T8551 det the,modulation
R7603 T8397 T8401 amod greater,activation
R7604 T8551 T8549 pobj modulation,of
R7605 T8552 T8551 prep of,modulation
R7606 T8553 T8554 amod inflammatory,responses
R7607 T8554 T8552 pobj responses,of
R7608 T8398 T8400 compound NF-κB,AP-1
R7609 T8555 T8554 prep by,responses
R7610 T8399 T8400 compound /,AP-1
R7611 T8556 T8555 pobj CFTR,by
R7612 T8400 T8401 compound AP-1,activation
R7613 T8557 T8507 punct .,are
R7614 T8558 T8562 nsubjpass Consideration,given
R7615 T8559 T8562 aux must,given
R7616 T8401 T8396 dobj activation,generated
R7617 T8560 T8562 advmod also,given
R7618 T8561 T8562 auxpass be,given
R7619 T8562 T8562 ROOT given,given
R7620 T8402 T8404 punct (,6a
R7621 T8563 T8562 dative to,given
R7622 T8564 T8565 det the,nature
R7623 T8403 T8404 compound Fig.,6a
R7624 T8565 T8563 pobj nature,to
R7625 T8566 T8565 prep of,nature
R7626 T8567 T8569 nmod P.,infection
R7627 T8404 T8401 appos 6a,activation
R7628 T8568 T8569 compound aeruginosa,infection
R7702 T8421 T8376 punct .,is
R7703 T8626 T8627 nummod 60,]
R7704 T8627 T8623 pobj ],as
R7705 T8628 T8612 punct .,established
R7706 T8422 T8424 nsubj This,be
R7707 T8423 T8424 aux may,be
R7708 T8424 T8424 ROOT be,be
R7709 T8425 T8424 acomp indicative,be
R7710 T8629 T8645 prep By,confirmed
R7711 T8630 T8629 pcomp employing,By
R7712 T8426 T8425 prep of,indicative
R7713 T8631 T8632 det a,deletion
R7714 T8632 T8630 dobj deletion,employing
R7715 T8633 T8632 amod mutant,deletion
R7716 T8634 T8632 prep in,deletion
R7717 T8427 T8429 det the,degree
R7718 T8428 T8429 amod different,degree
R7719 T8635 T8634 pobj exsA,in
R7720 T8429 T8426 pobj degree,of
R7721 T8636 T8635 punct ",",exsA
R7722 T8637 T8639 det the,regulator
R7723 T8638 T8639 amod key,regulator
R7724 T8430 T8429 cc and,degree
R7725 T8639 T8635 appos regulator,exsA
R7726 T8640 T8639 prep in,regulator
R7727 T8641 T8642 nummod T3SS,transcription
R7728 T8431 T8429 conj quality,degree
R7729 T8642 T8640 pobj transcription,in
R7730 T8643 T8645 punct ",",confirmed
R7731 T8644 T8645 nsubj we,confirmed
R7732 T8645 T8645 ROOT confirmed,confirmed
R7733 T8646 T8649 mark that,is
R7734 T8432 T8431 prep of,quality
R7735 T8647 T8648 det the,T3SS
R7736 T8648 T8649 nsubj T3SS,is
R7737 T8649 T8645 ccomp is,confirmed
R7738 T8650 T8649 acomp important,is
R7739 T8433 T8435 det the,response
R7740 T8651 T8650 prep for,important
R7741 T8652 T8653 amod IL-1β,secretion
R7742 T8653 T8651 pobj secretion,for
R7743 T8434 T8435 amod inflammatory,response
R7744 T8654 T8655 punct (,Fig.
R7745 T8655 T8649 npadvmod Fig.,is
R7746 T8656 T8655 nummod 5a,Fig.
R7747 T8726 T8725 cc and,ATP
R7748 T8657 T8655 appos b,Fig.
R7749 T8658 T8655 punct ),Fig.
R7750 T8659 T8655 punct ",",Fig.
R7751 T8660 T8649 cc and,is
R7752 T8727 T8728 compound P.,aeruginosa
R7753 T8661 T8676 mark that,be
R7754 T8662 T8676 advcl depending,be
R7755 T8663 T8662 prep on,depending
R7756 T8728 T8725 conj aeruginosa,ATP
R7757 T8664 T8665 det the,adaptation
R7758 T8665 T8663 pobj adaptation,on
R7759 T8666 T8665 prep in,adaptation
R7760 T8667 T8666 pobj type,in
R7761 T8729 T8697 punct .,are
R7762 T8668 T8669 compound III,secretion
R7763 T8669 T8662 dep secretion,depending
R7764 T8670 T8676 punct ",",be
R7765 T8730 T8733 advmod Furthermore,find
R7766 T8671 T8674 det the,response
R7767 T8672 T8674 compound host,response
R7768 T8673 T8674 compound IL-1β,response
R7769 T8731 T8733 punct ",",find
R7770 T8674 T8676 nsubj response,be
R7771 T8675 T8676 aux may,be
R7772 T8676 T8649 conj be,is
R7670 T8601 T8600 punct ",",]
R7671 T8414 T8415 amod live,bacteria
R7672 T8602 T8600 conj [,]
R7673 T8603 T8604 nummod 20,]
R7674 T8604 T8600 conj ],]
R7675 T8415 T8413 pobj bacteria,than
R7676 T8605 T8582 punct .,mediates
R7677 T8606 T8612 advmod However,established
R7678 T8607 T8612 punct ",",established
R7679 T8416 T8396 prep despite,generated
R7680 T8608 T8612 nsubj clones,established
R7681 T8609 T8608 prep of,clones
R7682 T8610 T8611 compound P.,aeruginosa
R7683 T8611 T8609 pobj aeruginosa,of
R7684 T8612 T8612 ROOT established,established
R7685 T8417 T8416 pobj stimulation,despite
R7686 T8613 T8612 prep during,established
R7687 T8614 T8615 amod chronic,infection
R7688 T8615 T8613 pobj infection,during
R7689 T8418 T8417 prep at,stimulation
R7690 T8616 T8617 aux may,accumulate
R7691 T8617 T8612 conj accumulate,established
R7692 T8618 T8617 dobj mutations,accumulate
R7693 T8619 T8618 prep in,mutations
R7694 T8419 T8420 amod equivalent,MOIs
R7695 T8620 T8621 compound virulence,factors
R7696 T8621 T8619 pobj factors,in
R7697 T8622 T8623 amod such,as
R7698 T8420 T8418 pobj MOIs,at
R7699 T8623 T8621 prep as,factors
R7700 T8624 T8625 compound exsA,[
R7701 T8625 T8627 nmod [,]
R8189 T9373 T9378 nsubjpass Ethics,obtained
R8190 T9374 T9375 compound Statement,Blood
R8191 T9375 T9376 compound Blood,samples
R8192 T9376 T9378 nsubjpass samples,obtained
R8193 T9377 T9378 auxpass were,obtained
R8194 T9378 T9378 ROOT obtained,obtained
R8195 T9379 T9378 prep with,obtained
R8196 T9380 T9382 amod informed,consent
R8197 T9381 T9382 amod written,consent
R8198 T9382 T9379 pobj consent,with
R8199 T9383 T9382 prep from,consent
R8200 T9384 T9385 compound control,subjects
R8201 T9385 T9383 pobj subjects,from
R8202 T9386 T9385 cc and,subjects
R8203 T9387 T9388 compound CF,patients
R8204 T9388 T9385 conj patients,subjects
R8205 T9389 T9385 prep at,subjects
R8206 T9390 T9392 det the,Children
R8207 T9391 T9392 compound BC,Children
R8208 T9392 T9394 poss Children,Hospital
R8209 T9393 T9392 case 's,Children
R8210 T9394 T9389 pobj Hospital,at
R8211 T9395 T9378 punct .,obtained
R8212 T9396 T9398 nsubjpass Consent,obtained
R8213 T9397 T9398 auxpass was,obtained
R8214 T9398 T9398 ROOT obtained,obtained
R8215 T9399 T9398 prep for,obtained
R8216 T9400 T9399 pobj children,for
R8217 T9401 T9398 agent by,obtained
R8218 T9402 T9403 poss their,parent
R8219 T9403 T9401 pobj parent,by
R8220 T9404 T9403 cc or,parent
R8221 T9405 T9406 amod legal,guardian
R8222 T9406 T9403 conj guardian,parent
R7773 T8732 T8733 nsubj we,find
R7774 T8677 T8676 advmod up,be
R7775 T8678 T8677 cc or,up
R7776 T8679 T8682 amod downregulated,]
R7777 T8733 T8733 ROOT find,find
R7778 T8680 T8682 nmod [,]
R7779 T8681 T8682 nummod 19,]
R7780 T8682 T8676 attr ],be
R7781 T8683 T8682 punct ",",]
R7782 T8684 T8686 nmod [,]
R7783 T8734 T8735 amod little,evidence
R7784 T8685 T8686 nummod 60,]
R7785 T8686 T8682 conj ],]
R7786 T8687 T8686 punct ",",]
R7787 T8688 T8686 conj [,]
R7788 T8735 T8733 dobj evidence,find
R7789 T8689 T8690 nummod 61,]
R7790 T8690 T8686 conj ],]
R7791 T8736 T8737 aux to,support
R7792 T8691 T8645 punct .,confirmed
R7793 T8692 T8697 prep In,are
R7794 T8693 T8692 pobj conclusion,In
R7795 T8694 T8697 punct ",",are
R7796 T8695 T8696 poss our,data
R7797 T8696 T8697 nsubj data,are
R7798 T8697 T8697 ROOT are,are
R7799 T8698 T8697 acomp consistent,are
R7800 T8699 T8698 prep with,consistent
R7801 T8700 T8701 det a,role
R7802 T8701 T8699 pobj role,with
R7803 T8702 T8701 prep for,role
R7804 T8703 T8704 amod hematopoietic,cells
R7805 T8704 T8702 pobj cells,for
R7806 T8705 T8701 punct ",",role
R7807 T8706 T8707 neg not,airway
R7808 T8707 T8697 attr airway,are
R7809 T8708 T8709 amod epithelial,cells
R7810 T8737 T8735 relcl support,evidence
R7811 T8709 T8707 dobj cells,airway
R7812 T8710 T8707 punct ",",airway
R7813 T8711 T8707 prep as,airway
R7814 T8738 T8742 det an,response
R7815 T8712 T8714 det the,source
R7816 T8713 T8714 amod major,source
R7817 T8739 T8742 amod increased,response
R7818 T8714 T8711 pobj source,as
R7819 T8740 T8742 amod IL-1β,response
R7820 T8715 T8714 prep of,source
R7821 T8716 T8718 amod inflammasome-mediated,production
R7822 T8741 T8742 amod inflammatory,response
R7823 T8717 T8718 compound IL-1β,production
R7824 T8718 T8715 pobj production,of
R7825 T8742 T8737 dobj response,support
R7826 T8719 T8718 prep in,production
R7827 T8720 T8721 det the,lungs
R7828 T8721 T8719 pobj lungs,in
R7829 T8743 T8742 prep to,response
R7830 T8722 T8714 prep in,source
R7831 T8723 T8722 pobj response,in
R7832 T8724 T8723 prep to,response
R7833 T8725 T8724 pobj ATP,to
R7834 T8744 T8747 compound NF-κB,stimulation
R7835 T8745 T8746 compound /,Inflammasome
R7836 T8746 T8747 compound Inflammasome,stimulation
R7837 T8747 T8743 pobj stimulation,to
R7838 T8748 T8747 prep in,stimulation
R7839 T8749 T8750 compound CF,patients
R7840 T8750 T8748 pobj patients,in
R7841 T8751 T8733 punct .,find
R7842 T8752 T8753 amod Further,studies
R7843 T8753 T8755 nsubjpass studies,warranted
R7844 T8754 T8755 auxpass are,warranted
R7845 T8755 T8755 ROOT warranted,warranted
R7846 T8756 T8757 aux to,determine
R7847 T8757 T8755 advcl determine,warranted
R7848 T8758 T8779 mark if,correlated
R7849 T8759 T8779 nsubjpass adaptations,correlated
R7850 T8760 T8759 prep of,adaptations
R7851 T8761 T8762 compound P.,aeruginosa
R7852 T8762 T8760 pobj aeruginosa,of
R7853 T8763 T8759 prep during,adaptations
R7854 T8764 T8765 det the,course
R7855 T8765 T8763 pobj course,during
R7856 T8766 T8765 prep of,course
R7857 T8767 T8769 amod chronic,infection
R7858 T8768 T8769 compound lung,infection
R7859 T8769 T8770 compound infection,alters
R7860 T8770 T8772 nmod alters,activation
R7861 T8771 T8772 amod inflammasome,activation
R7862 T8772 T8766 pobj activation,of
R7863 T8773 T8759 punct ",",adaptations
R7864 T8774 T8759 cc and,adaptations
R7865 T8775 T8779 mark whether,correlated
R7866 T8776 T8779 nsubjpass this,correlated
R7867 T8777 T8779 aux can,correlated
R7868 T8778 T8779 auxpass be,correlated
R7869 T8779 T8757 advcl correlated,determine
R7870 T8780 T8779 prep with,correlated
R7871 T8781 T8782 compound disease,severity
R7872 T8782 T8780 pobj severity,with
R7873 T8783 T8782 prep in,severity
R7874 T8784 T8783 pobj CF.,in
R8233 T9417 T9418 aux to,provide
R8234 T9418 T9416 xcomp provide,required
R8235 T9419 T9420 amod informed,assent
R8236 T9420 T9418 dobj assent,provide
R8237 T9421 T9422 advmod as,well
R8238 T9422 T9418 advmod well,provide
R8239 T9423 T9416 punct .,required
R8240 T9424 T9426 nsubjpass Protocols,approved
R8241 T9425 T9426 auxpass were,approved
R8242 T9426 T9426 ROOT approved,approved
R8243 T9427 T9426 agent by,approved
R8244 T9428 T9432 det the,Board
R8245 T9429 T9432 compound Clinical,Board
R8246 T9430 T9431 compound Research,Ethics
R8247 T9431 T9432 compound Ethics,Board
R8248 T9432 T9427 pobj Board,by
R8249 T9433 T9432 punct (,Board
R8250 T9434 T9432 appos H09-01192,Board
R8251 T9435 T9432 punct ),Board
R8252 T9436 T9426 punct .,approved
R8670 T9881 T9883 compound Cell,CF
R8671 T9882 T9883 compound Culture,CF
R8672 T9883 T9898 nsubjpass CF,obtained
R8673 T9884 T9883 punct (,CF
R8674 T9885 T9896 nmod IB3-1,cells
R8675 T9886 T9885 cc and,IB3-1
R8676 T9887 T9885 conj CuFi-1,IB3-1
R8677 T9888 T9887 punct ),CuFi-1
R8678 T9889 T9885 cc and,IB3-1
R8679 T9890 T9885 conj control,IB3-1
R8680 T9891 T9890 punct (,control
R8681 T9892 T9890 appos S9,control
R8682 T9893 T9885 cc and,IB3-1
R8683 T9894 T9885 conj NuLi-1,IB3-1
R8684 T9895 T9896 punct ),cells
R8685 T9896 T9898 nsubjpass cells,obtained
R8686 T9897 T9898 auxpass were,obtained
R8687 T9898 T9898 ROOT obtained,obtained
R8688 T9899 T9898 prep from,obtained
R8689 T9900 T9904 det the,Collection
R8690 T9901 T9904 compound American,Collection
R8691 T9902 T9904 compound Type,Collection
R8692 T9903 T9904 compound Culture,Collection
R8693 T9904 T9899 pobj Collection,from
R8694 T9905 T9898 punct .,obtained
R8695 T9906 T9907 nummod IB3-1,cells
R8696 T9907 T9909 nsubjpass cells,derived
R8697 T9908 T9909 auxpass were,derived
R8698 T9909 T9909 ROOT derived,derived
R8699 T9910 T9909 prep from,derived
R8700 T9911 T9912 det a,patient
R8701 T9912 T9910 pobj patient,from
R8702 T9913 T9912 acl expressing,patient
R8703 T9914 T9915 det the,ΔF508
R8704 T9915 T9913 dobj ΔF508,expressing
R8223 T9407 T9398 punct .,obtained
R8224 T9408 T9416 nsubjpass Subjects,required
R8225 T9409 T9410 nummod 7,years
R8226 T9410 T9408 dobj years,Subjects
R8227 T9411 T9410 prep of,years
R8228 T9412 T9411 pobj age,of
R8229 T9413 T9410 cc and,years
R8230 T9414 T9416 nsubjpass older,required
R8231 T9415 T9416 auxpass were,required
R8232 T9416 T9416 ROOT required,required
R8744 T9955 T9957 amod wild-type,genotype
R8745 T9956 T9957 amod CFTR,genotype
R8746 T9957 T9953 dobj genotype,possessing
R8747 T9958 T9949 punct .,derived
R8748 T9959 T9960 amod THP1-XBlue,cells
R8749 T9960 T9962 nsubj cells,express
R8750 T9961 T9962 advmod stably,express
R8751 T9962 T9962 ROOT express,express
R8752 T9963 T9967 det a,phosphatase
R8753 T9964 T9967 amod secreted,phosphatase
R8754 T9965 T9967 amod embryonic,phosphatase
R8755 T9966 T9967 compound alkaline,phosphatase
R8756 T9967 T9962 dobj phosphatase,express
R8757 T9968 T9967 punct (,phosphatase
R8758 T9969 T9967 appos SEAP,phosphatase
R8759 T9970 T9967 punct ),phosphatase
R8760 T9971 T9972 compound reporter,inducible
R8761 T9972 T9967 appos inducible,phosphatase
R8762 T9973 T9972 prep by,inducible
R8763 T9974 T9973 pobj NF-κB,by
R8764 T9975 T9974 cc and,NF-κB
R8765 T9976 T9974 conj AP-1,NF-κB
R8766 T9977 T9978 punct (,Invivogen
R8767 T9978 T9976 appos Invivogen,AP-1
R8768 T9979 T9976 punct ),AP-1
R8769 T9980 T9962 punct .,express
R8770 T9981 T9983 nsubjpass Cells,cultured
R8771 T9982 T9983 auxpass were,cultured
R8772 T9983 T9983 ROOT cultured,cultured
R8773 T9984 T9985 mark as,recommended
R8774 T9985 T9983 advcl recommended,cultured
R8775 T9986 T9985 agent by,recommended
R8776 T9987 T9989 poss their,suppliers
R8777 T9988 T9989 amod respective,suppliers
R8778 T9989 T9986 pobj suppliers,by
R8779 T9990 T9989 acl using,suppliers
R8780 T9991 T9992 amod standard,protocols
R8781 T9992 T9990 dobj protocols,using
R8782 T9993 T9983 punct .,cultured
R8783 T9994 T9997 nummod S9,cells
R8784 T9995 T9994 cc and,S9
R8785 T9996 T9994 conj IB3-1,S9
R8786 T9997 T9999 nsubjpass cells,cultured
R8787 T9998 T9999 auxpass were,cultured
R8788 T9999 T9999 ROOT cultured,cultured
R8789 T10000 T9999 prep in,cultured
R8790 T10001 T10002 amod basal,LHC-8
R8791 T10002 T10000 pobj LHC-8,in
R8792 T10003 T10004 punct (,Invitrogen
R8793 T10004 T10002 appos Invitrogen,LHC-8
R8794 T10005 T9999 punct ),cultured
R8795 T10006 T9999 prep supplemented,cultured
R8796 T10007 T10006 prep with,supplemented
R8797 T10008 T10009 nummod 10,%
R8798 T10009 T10007 pobj %,with
R8799 T10010 T10011 punct (,v/v
R8800 T10011 T10009 appos v/v,%
R8801 T10012 T10011 punct ),v/v
R8802 T10013 T10015 amod fetal,serum
R8705 T9916 T9915 cc and,ΔF508
R8706 T9917 T9915 conj W1282X,ΔF508
R8707 T9918 T9915 conj mutations,ΔF508
R8708 T9919 T9918 cc and,mutations
R8709 T9920 T9918 conj CuFi-1,mutations
R8710 T9921 T9922 auxpass were,derived
R8711 T9922 T9909 conj derived,derived
R8712 T9923 T9922 prep from,derived
R8713 T9924 T9927 det a,patient
R8714 T9925 T9927 nmod ΔF508,patient
R8715 T9926 T9927 amod homozygous,patient
R8716 T9927 T9923 pobj patient,from
R8717 T9928 T9909 punct .,derived
R8718 T9929 T9930 nummod S9,cells
R8719 T9930 T9931 nsubj cells,are
R8720 T9931 T9931 ROOT are,are
R8721 T9932 T9933 amod IB3-1,cells
R8722 T9933 T9931 attr cells,are
R8723 T9934 T9937 nsubjpass that,transfected
R8724 T9935 T9937 aux have,transfected
R8725 T9936 T9937 auxpass been,transfected
R8726 T9937 T9933 relcl transfected,cells
R8727 T9938 T9937 prep with,transfected
R8728 T9939 T9940 nsubj CFTR,using
R8729 T9940 T9938 pcomp using,with
R8730 T9941 T9949 det an,derived
R8731 T9942 T9944 amod adeno-associated,vector
R8732 T9943 T9944 amod viral,vector
R8733 T9944 T9949 nsubjpass vector,derived
R8734 T9945 T9944 cc and,vector
R8735 T9946 T9944 conj NuLi-1,vector
R8736 T9947 T9944 conj cells,vector
R8737 T9948 T9949 auxpass were,derived
R8738 T9949 T9931 advcl derived,are
R8739 T9950 T9949 prep from,derived
R8740 T9951 T9952 det a,patient
R8741 T9952 T9950 pobj patient,from
R8742 T9953 T9952 acl possessing,patient
R8743 T9954 T9957 det a,genotype
R8818 T10029 T9999 punct .,cultured
R8819 T10030 T10033 amod NuLi-1,cells
R8820 T10031 T10033 compound /,cells
R8821 T10032 T10033 compound CuFi-1,cells
R8822 T10033 T10034 nsubj cells,were
R8823 T10034 T10034 ROOT were,were
R8824 T10035 T10034 acomp cultured,were
R8825 T10036 T10035 prep in,cultured
R8826 T10037 T10039 nmod BEBM,medium
R8827 T10038 T10039 amod serum-free,medium
R8828 T10039 T10036 pobj medium,in
R8829 T10040 T10041 punct (,Lonza
R8830 T10041 T10039 appos Lonza,medium
R8831 T10042 T10035 punct ),cultured
R8832 T10043 T10034 prep with,were
R8833 T10044 T10046 compound supplement,kit
R8834 T10045 T10046 compound bullet,kit
R8835 T10046 T10043 pobj kit,with
R8836 T10047 T10046 punct (,kit
R8837 T10048 T10050 nmod EGF,hydrocortisone
R8838 T10049 T10050 punct ",",hydrocortisone
R8839 T10050 T10046 appos hydrocortisone,kit
R8840 T10051 T10050 punct ",",hydrocortisone
R8841 T10052 T10054 amod bovine,extract
R8842 T10053 T10054 amod pituitary,extract
R8843 T10054 T10050 conj extract,hydrocortisone
R8844 T10055 T10054 punct ",",extract
R8845 T10056 T10050 conj transferrin,hydrocortisone
R8846 T10057 T10056 punct ",",transferrin
R8847 T10058 T10059 amod bovine,insulin
R8848 T10059 T10056 conj insulin,transferrin
R8849 T10060 T10059 punct ",",insulin
R8850 T10061 T10059 conj triiodothyronine,insulin
R8851 T10062 T10061 punct ",",triiodothyronine
R8852 T10063 T10061 conj epinephrine,triiodothyronine
R8853 T10064 T10063 punct ",",epinephrine
R8854 T10065 T10066 amod retinoic,acid
R8855 T10066 T10063 appos acid,epinephrine
R8856 T10067 T10063 punct ),epinephrine
R8857 T10068 T10061 punct ",",triiodothyronine
R8858 T10069 T10071 nummod 2,L-glutamine
R8859 T10070 T10071 compound mM,L-glutamine
R8860 T10071 T10061 conj L-glutamine,triiodothyronine
R8861 T10072 T10071 punct ",",L-glutamine
R8862 T10073 T10071 cc and,L-glutamine
R8863 T10074 T10077 nummod 1,pyruvate
R8864 T10075 T10077 compound mM,pyruvate
R8865 T10076 T10077 compound sodium,pyruvate
R8866 T10077 T10071 conj pyruvate,L-glutamine
R8867 T10078 T10077 punct .,pyruvate
R8868 T10079 T10085 nsubj PBMCs,were
R8869 T10080 T10079 prep from,PBMCs
R8870 T10081 T10082 compound CF,patients
R8871 T10082 T10080 pobj patients,from
R8872 T10083 T10082 cc and,patients
R8873 T10084 T10082 conj controls,patients
R8874 T10085 T10085 ROOT were,were
R8875 T10086 T10085 acomp cultured,were
R8876 T10087 T10086 prep in,cultured
R8877 T10088 T10087 pobj RPMI-1640,in
R8878 T10089 T10088 punct (,RPMI-1640
R8879 T10090 T10088 appos Hyclone,RPMI-1640
R8880 T10091 T10088 punct ),RPMI-1640
R8881 T10092 T10086 conj supplemented,cultured
R8882 T10093 T10092 prep with,supplemented
R8883 T10094 T10095 nummod 10,%
R8884 T10095 T10096 compound %,FBS
R8885 T10096 T10093 pobj FBS,with
R8886 T10097 T10096 punct ",",FBS
R8887 T10098 T10100 nummod 2,L-glutamine
R8888 T10099 T10100 compound mM,L-glutamine
R8889 T10100 T10096 conj L-glutamine,FBS
R8890 T10101 T10100 punct ",",L-glutamine
R8891 T10102 T10100 cc and,L-glutamine
R8892 T10103 T10106 nummod 1,pyruvate
R8893 T10104 T10106 compound mM,pyruvate
R8894 T10105 T10106 compound sodium,pyruvate
R8895 T10106 T10100 conj pyruvate,L-glutamine
R8896 T10107 T10106 punct (,pyruvate
R8897 T10108 T10109 amod complete,RPMI
R8898 T10109 T10106 appos RPMI,pyruvate
R8899 T10110 T10106 punct ),pyruvate
R8900 T10111 T10085 punct .,were
R8901 T10112 T10113 amod THP1-XBlue,cells
R8902 T10113 T10114 nsubj cells,were
R8903 T10114 T10114 ROOT were,were
R8904 T10115 T10114 acomp cultured,were
R8905 T10116 T10115 prep in,cultured
R8906 T10117 T10118 amod complete,RPMI
R8907 T10118 T10116 pobj RPMI,in
R8908 T10119 T10115 prep with,cultured
R8909 T10120 T10121 det the,addition
R8910 T10121 T10119 pobj addition,with
R8911 T10122 T10121 prep of,addition
R8912 T10123 T10122 pobj zeocin,of
R8913 T10124 T10123 punct (,zeocin
R8914 T10125 T10126 nummod 100,µg
R8915 T10126 T10128 nmod µg,ml
R8916 T10127 T10128 compound /,ml
R8917 T10128 T10123 appos ml,zeocin
R8918 T10129 T10128 punct ),ml
R8919 T10130 T10131 aux to,select
R8920 T10131 T10115 advcl select,cultured
R8921 T10132 T10131 prep for,select
R8922 T10133 T10132 pobj cells,for
R8923 T10134 T10133 acl expressing,cells
R8924 T10135 T10140 det the,reporter
R8925 T10136 T10139 compound SEAP,AP-1
R8926 T10137 T10139 compound NF-κB,AP-1
R8927 T10138 T10139 compound /,AP-1
R8928 T10139 T10140 compound AP-1,reporter
R8929 T10140 T10134 dobj reporter,expressing
R8930 T10141 T10114 punct .,were
R8931 T10142 T10151 advmod Prior,plated
R8932 T10143 T10151 prep to,plated
R8933 T10144 T10143 pobj stimulation,to
R8934 T10145 T10151 punct ",",plated
R8935 T10146 T10149 amod bronchial,lines
R8936 T10147 T10149 amod epithelial,lines
R8803 T10014 T10015 amod bovine,serum
R8804 T10015 T10009 appos serum,%
R8805 T10016 T10017 punct (,FBS
R8806 T10017 T10015 parataxis FBS,serum
R8807 T10018 T10017 punct ),FBS
R8808 T10019 T10015 punct ",",serum
R8809 T10020 T10022 nummod 2,L-glutamine
R8810 T10021 T10022 compound mM,L-glutamine
R8811 T10022 T10015 appos L-glutamine,serum
R8812 T10023 T10015 punct ",",serum
R8813 T10024 T10015 cc and,serum
R8814 T10025 T10026 nummod 1,mM
R8815 T10026 T10028 compound mM,pyruvate
R8816 T10027 T10028 compound sodium,pyruvate
R8817 T10028 T10015 conj pyruvate,serum
R9033 T10244 T10229 punct .,coated
R9034 T10245 T10247 nsubjpass PBMCs,plated
R9035 T10246 T10247 auxpass were,plated
R9036 T10247 T10247 ROOT plated,plated
R9037 T10248 T10247 prep in,plated
R9038 T10249 T10250 amod 96-well,plates
R9039 T10250 T10248 pobj plates,in
R9040 T10251 T10247 prep at,plated
R9041 T10252 T10253 det a,density
R9042 T10253 T10251 pobj density,at
R9043 T10254 T10253 prep of,density
R9044 T10255 T10258 nummod 1.5,cells/well
R9045 T10256 T10258 nmod ×,cells/well
R9046 T10257 T10258 nummod 105,cells/well
R9047 T10258 T10254 pobj cells/well,of
R9048 T10259 T10247 prep in,plated
R9049 T10260 T10261 nummod 200,µl
R9050 T10261 T10259 pobj µl,in
R9051 T10262 T10261 punct (,µl
R9052 T10263 T10266 nummod 7.5,cells/mL
R9053 T10264 T10266 nummod ×,cells/mL
R9054 T10265 T10266 nummod 105,cells/mL
R9055 T10266 T10261 appos cells/mL,µl
R9056 T10267 T10261 punct ),µl
R9057 T10268 T10247 punct .,plated
R9058 T10269 T10271 nummod THP-1,cells
R9059 T10270 T10271 compound reporter,cells
R9060 T10271 T10273 nsubjpass cells,differentiated
R9061 T10272 T10273 auxpass were,differentiated
R9062 T10273 T10273 ROOT differentiated,differentiated
R9063 T10274 T10273 prep into,differentiated
R9064 T10275 T10277 det a,phenotype
R9065 T10276 T10277 amod macrophage-like,phenotype
R9066 T10277 T10274 pobj phenotype,into
R9067 T10278 T10273 advcl using,differentiated
R9068 T10279 T10280 nummod 50,ng/ml
R9069 T10280 T10278 dobj ng/ml,using
R9070 T10281 T10280 prep of,ng/ml
R9071 T10282 T10284 nmod phorbol,13-acetate
R9072 T10283 T10284 amod 12-myristate,13-acetate
R9073 T10284 T10281 pobj 13-acetate,of
R9074 T10285 T10286 punct (,PMA
R9075 T10286 T10284 appos PMA,13-acetate
R9076 T10287 T10284 punct ),13-acetate
R9077 T10288 T10290 punct (,Aldrich
R9078 T10289 T10290 compound Sigma,Aldrich
R9079 T10290 T10284 appos Aldrich,13-acetate
R9080 T10291 T10280 punct ),ng/ml
R9081 T10292 T10278 prep for,using
R9082 T10293 T10294 nummod 24,hours
R9083 T10294 T10292 pobj hours,for
R9084 T10295 T10278 prep at,using
R9085 T10296 T10297 det a,density
R9086 T10297 T10295 pobj density,at
R9087 T10298 T10297 prep of,density
R8937 T10148 T10149 compound cell,lines
R8938 T10149 T10151 nsubjpass lines,plated
R8939 T10150 T10151 auxpass were,plated
R8940 T10151 T10151 ROOT plated,plated
R8941 T10152 T10151 prep in,plated
R8942 T10153 T10158 amod coated,plates
R8943 T10154 T10158 nmod [,plates
R8944 T10155 T10158 nummod 62,plates
R8945 T10156 T10158 nmod ],plates
R8946 T10157 T10158 compound 96-well,plates
R8947 T10158 T10152 pobj plates,in
R8948 T10159 T10161 punct (,Biosciences
R8949 T10160 T10161 compound BD,Biosciences
R8950 T10161 T10158 appos Biosciences,plates
R8951 T10162 T10161 punct ),Biosciences
R8952 T10163 T10151 prep at,plated
R8953 T10164 T10167 nummod 3,cells/well
R8954 T10165 T10167 nummod ×,cells/well
R8955 T10166 T10167 nummod 104,cells/well
R8956 T10167 T10163 pobj cells/well,at
R8957 T10168 T10169 mark unless,indicated
R8958 T10169 T10151 advcl indicated,plated
R8959 T10170 T10151 punct ",",plated
R8960 T10171 T10151 cc and,plated
R8961 T10172 T10151 conj allowed,plated
R8962 T10173 T10174 aux to,adhere
R8963 T10174 T10172 xcomp adhere,allowed
R8964 T10175 T10174 advmod overnight,adhere
R8965 T10176 T10151 punct .,plated
R8966 T10177 T10184 nsubjpass Plates,coated
R8967 T10178 T10177 prep for,Plates
R8968 T10179 T10178 pobj S9,for
R8969 T10180 T10179 cc and,S9
R8970 T10181 T10179 conj IB3-1,S9
R8971 T10182 T10184 nsubjpass stimulations,coated
R8972 T10183 T10184 auxpass were,coated
R8973 T10184 T10184 ROOT coated,coated
R8974 T10185 T10184 prep in,coated
R8975 T10186 T10187 det a,mixture
R8976 T10187 T10185 pobj mixture,in
R8977 T10188 T10187 prep of,mixture
R8978 T10189 T10190 amod bovine,serum
R8979 T10190 T10191 compound serum,albumin
R8980 T10191 T10188 pobj albumin,of
R8981 T10192 T10191 punct (,albumin
R8982 T10193 T10194 nummod 100,µg
R8983 T10194 T10196 compound µg,ml
R8984 T10195 T10196 compound /,ml
R8985 T10196 T10191 appos ml,albumin
R8986 T10197 T10191 punct ),albumin
R8987 T10198 T10184 punct ",",coated
R8988 T10199 T10184 dep fibronectin,coated
R8989 T10200 T10199 punct (,fibronectin
R8990 T10201 T10202 nummod 10,µg
R8991 T10202 T10199 appos µg,fibronectin
R8992 T10203 T10204 compound /,ml
R8993 T10204 T10202 appos ml,µg
R8994 T10205 T10202 punct ),µg
R8995 T10206 T10199 punct ",",fibronectin
R8996 T10207 T10199 cc and,fibronectin
R8997 T10208 T10209 amod bovine,collage
R8998 T10209 T10210 compound collage,type
R8999 T10210 T10199 conj type,fibronectin
R9000 T10211 T10199 nsubj I,fibronectin
R9001 T10212 T10211 punct (,I
R9002 T10213 T10214 nummod 30,µg
R9003 T10214 T10216 compound µg,ml
R9004 T10215 T10216 compound /,ml
R9005 T10216 T10211 appos ml,I
R9006 T10217 T10211 punct ),I
R9007 T10218 T10220 punct (,Biosciences
R9008 T10219 T10220 compound BD,Biosciences
R9009 T10220 T10211 appos Biosciences,I
R9010 T10221 T10220 punct ),Biosciences
R9011 T10222 T10184 punct .,coated
R9012 T10223 T10229 nsubjpass Plates,coated
R9013 T10224 T10223 prep for,Plates
R9014 T10225 T10224 pobj NuLi-1,for
R9015 T10226 T10225 cc and,NuLi-1
R9016 T10227 T10225 conj CuFi-1,NuLi-1
R9017 T10228 T10229 auxpass were,coated
R9018 T10229 T10229 ROOT coated,coated
R9019 T10230 T10229 prep with,coated
R9020 T10231 T10232 compound collagen,type
R9021 T10232 T10233 compound type,IV
R9022 T10233 T10230 pobj IV,with
R9023 T10234 T10233 punct (,IV
R9024 T10235 T10236 nummod 60,µg
R9025 T10236 T10238 compound µg,ml
R9026 T10237 T10238 compound /,ml
R9027 T10238 T10233 appos ml,IV
R9028 T10239 T10233 punct ),IV
R9029 T10240 T10233 punct (,IV
R9030 T10241 T10242 compound Sigma,Aldrich
R9031 T10242 T10233 appos Aldrich,IV
R9032 T10243 T10233 punct ),IV
R9097 T10308 T10310 nummod ×,cells/ml
R9098 T10309 T10310 nummod 105,cells/ml
R9099 T10310 T10305 appos cells/ml,µl
R9100 T10311 T10305 punct ),µl
R9101 T10312 T10273 punct .,differentiated
R9102 T10313 T10315 nsubjpass Cells,washed
R9103 T10314 T10315 auxpass were,washed
R9104 T10315 T10315 ROOT washed,washed
R9105 T10316 T10315 prep with,washed
R9106 T10317 T10316 pobj PBS,with
R9107 T10318 T10315 cc and,washed
R9108 T10319 T10315 conj allowed,washed
R9109 T10320 T10321 aux to,rest
R9110 T10321 T10319 xcomp rest,allowed
R9111 T10322 T10326 det a,prior
R9112 T10323 T10326 amod further,prior
R9113 T10324 T10325 nummod 42,hours
R9114 T10325 T10326 npadvmod hours,prior
R9115 T10326 T10321 advmod prior,rest
R9116 T10327 T10326 prep to,prior
R9117 T10328 T10327 pobj stimulation,to
R9118 T10329 T10315 punct .,washed
R9348 T10630 T10640 nsubjpass CF,established
R9349 T10631 T10630 cc and,CF
R9350 T10632 T10633 nsubj control,subject
R9351 T10633 T10640 advcl subject,established
R9352 T10634 T10633 dobj PBMCs,subject
R9353 T10635 T10636 det The,diagnosis
R9354 T10636 T10640 nsubjpass diagnosis,established
R9355 T10637 T10636 prep of,diagnosis
R9356 T10638 T10637 pobj CF,of
R9357 T10639 T10640 auxpass was,established
R9358 T10640 T10640 ROOT established,established
R9359 T10641 T10640 agent by,established
R9360 T10642 T10644 amod typical,features
R9361 T10643 T10644 amod clinical,features
R9362 T10644 T10641 pobj features,by
R9363 T10645 T10640 punct ",",established
R9364 T10646 T10640 dep increased,established
R9365 T10647 T10648 compound sweat,chloride
R9366 T10648 T10649 compound chloride,concentrations
R9367 T10649 T10646 dobj concentrations,increased
R9368 T10650 T10649 punct (,concentrations
R9369 T10651 T10649 appos >,concentrations
R9370 T10652 T10653 nummod 60,mmol/l
R9371 T10653 T10651 appos mmol/l,>
R9372 T10654 T10653 punct ),mmol/l
R9373 T10655 T10651 punct ",",>
R9374 T10656 T10651 cc and,>
R9375 T10657 T10651 conj detection,>
R9376 T10658 T10657 prep of,detection
R9377 T10659 T10660 amod CF-inducing,mutations
R9378 T10660 T10658 pobj mutations,of
R9379 T10661 T10640 punct .,established
R9380 T10662 T10663 det All,patients
R9381 T10663 T10666 nsubj patients,were
R9382 T10664 T10663 prep with,patients
R9383 T10665 T10664 pobj CF,with
R9384 T10666 T10666 ROOT were,were
R9385 T10667 T10668 advmod clinically,stable
R9386 T10668 T10666 acomp stable,were
R9088 T10299 T10302 nummod 1,cells/well
R9089 T10300 T10302 nummod ×,cells/well
R9090 T10301 T10302 nummod 105,cells/well
R9091 T10302 T10298 pobj cells/well,of
R9092 T10303 T10278 prep in,using
R9093 T10304 T10305 nummod 200,µl
R9094 T10305 T10303 pobj µl,in
R9095 T10306 T10305 punct (,µl
R9096 T10307 T10310 nummod 5,cells/ml
R9471 T10753 T10752 advcl using,isolated
R9472 T10754 T10756 nmod density,centrifugation
R9473 T10755 T10756 compound gradient,centrifugation
R9474 T10756 T10753 dobj centrifugation,using
R9475 T10757 T10756 prep on,centrifugation
R9476 T10758 T10760 compound Ficoll-Paque,Plus
R9477 T10759 T10760 compound ™,Plus
R9478 T10760 T10757 pobj Plus,on
R9479 T10761 T10760 punct (,Plus
R9480 T10762 T10763 compound GE,Healthcare
R9481 T10763 T10760 appos Healthcare,Plus
R9482 T10764 T10760 punct ),Plus
R9483 T10765 T10740 punct .,collected
R9484 T10766 T10767 det The,layer
R9485 T10767 T10770 nsubj layer,was
R9486 T10768 T10767 acl containing,layer
R9487 T10769 T10768 dobj PBMCs,containing
R9488 T10770 T10770 ROOT was,was
R9489 T10771 T10770 acomp isolated,was
R9490 T10772 T10771 punct ",",isolated
R9491 T10773 T10771 conj washed,isolated
R9492 T10774 T10773 advmod twice,washed
R9493 T10775 T10773 prep in,washed
R9494 T10776 T10775 pobj PBS,in
R9495 T10777 T10773 cc and,washed
R9496 T10778 T10773 conj resuspended,washed
R9497 T10779 T10778 prep in,resuspended
R9498 T10780 T10781 amod complete,RPMI
R9499 T10781 T10779 pobj RPMI,in
R9500 T10782 T10770 punct .,was
R9501 T10783 T10785 nsubjpass Cells,enumerated
R9502 T10784 T10785 auxpass were,enumerated
R9503 T10785 T10785 ROOT enumerated,enumerated
R9504 T10786 T10785 agent by,enumerated
R9505 T10787 T10789 amod trypan,exclusion
R9506 T10788 T10789 amod blue,exclusion
R9507 T10789 T10786 pobj exclusion,by
R9508 T10790 T10785 advcl using,enumerated
R9509 T10791 T10795 det the,counter
R9510 T10792 T10795 nmod Countess,counter
R9511 T10793 T10795 amod automated,counter
R9512 T10794 T10795 compound cell,counter
R9513 T10795 T10790 dobj counter,using
R9514 T10796 T10795 punct (,counter
R9515 T10797 T10795 appos Invitrogen,counter
R9516 T10798 T10795 punct ),counter
R9517 T10799 T10785 punct .,enumerated
R9518 T10800 T10809 prep For,allowed
R9519 T10801 T10800 pobj derivation,For
R9520 T10802 T10801 prep of,derivation
R9521 T10803 T10802 pobj macrophages,of
R9522 T10804 T10801 prep from,derivation
R9523 T10805 T10804 pobj monocytes,from
R9524 T10806 T10809 punct ",",allowed
R9525 T10807 T10809 nsubjpass monocytes,allowed
R9526 T10808 T10809 auxpass were,allowed
R9527 T10809 T10809 ROOT allowed,allowed
R9528 T10810 T10811 aux to,adhere
R9529 T10811 T10809 xcomp adhere,allowed
R9530 T10812 T10811 prep to,adhere
R9531 T10813 T10812 pobj plastic,to
R9532 T10814 T10811 prep for,adhere
R9533 T10815 T10816 nummod 2,hours
R9534 T10816 T10814 pobj hours,for
R9535 T10817 T10811 prep in,adhere
R9536 T10818 T10817 pobj RPMI,in
R9537 T10819 T10825 dep 1640,removed
R9538 T10820 T10825 prep after,removed
R9539 T10821 T10820 pobj which,after
R9387 T10669 T10666 prep at,were
R9388 T10670 T10671 det the,time
R9389 T10671 T10669 pobj time,at
R9390 T10672 T10671 prep of,time
R9391 T10673 T10674 compound blood,donation
R9392 T10674 T10672 pobj donation,of
R9393 T10675 T10666 punct ",",were
R9394 T10676 T10666 cc and,were
R9395 T10677 T10678 nsubj we,excluded
R9396 T10678 T10666 conj excluded,were
R9397 T10679 T10680 det any,subjects
R9398 T10680 T10678 dobj subjects,excluded
R9399 T10681 T10683 nsubj who,receiving
R9400 T10682 T10683 aux were,receiving
R9401 T10683 T10680 relcl receiving,subjects
R9402 T10684 T10685 amod systemic,corticosteroids
R9403 T10685 T10683 dobj corticosteroids,receiving
R9404 T10686 T10680 amod due,subjects
R9405 T10687 T10686 prep to,due
R9406 T10688 T10690 amod potential,activity
R9407 T10689 T10690 amod immunomodulatory,activity
R9408 T10690 T10687 pobj activity,to
R9409 T10691 T10678 punct .,excluded
R9410 T10692 T10693 compound Control,samples
R9411 T10693 T10695 nsubjpass samples,provided
R9412 T10694 T10695 auxpass were,provided
R9413 T10695 T10695 ROOT provided,provided
R9414 T10696 T10695 agent by,provided
R9415 T10697 T10699 amod healthy,volunteers
R9416 T10698 T10699 compound adult,volunteers
R9417 T10699 T10696 pobj volunteers,by
R9418 T10700 T10695 punct .,provided
R9419 T10701 T10707 prep In,demonstrated
R9420 T10702 T10703 advmod previously,published
R9421 T10703 T10704 amod published,work
R9422 T10704 T10701 pobj work,In
R9423 T10705 T10707 nsubj we,demonstrated
R9424 T10706 T10707 aux have,demonstrated
R9425 T10707 T10729 ccomp demonstrated,age-match
R9426 T10708 T10712 mark that,are
R9427 T10709 T10711 amod TLR-mediated,responses
R9428 T10710 T10711 amod inflammatory,responses
R9429 T10711 T10712 nsubj responses,are
R9430 T10712 T10707 ccomp are,demonstrated
R9431 T10713 T10712 acomp stable,are
R9432 T10714 T10713 prep in,stable
R9433 T10715 T10714 pobj humans,in
R9434 T10716 T10712 prep from,are
R9435 T10717 T10716 pobj birth,from
R9436 T10718 T10712 prep to,are
R9437 T10719 T10720 nummod 60,years
R9438 T10720 T10721 npadvmod years,old
R9439 T10721 T10718 amod old,to
R9440 T10722 T10724 nmod [,]
R9441 T10723 T10724 nummod 63,]
R9442 T10724 T10718 pobj ],to
R9443 T10725 T10729 punct ",",age-match
R9444 T10726 T10729 advmod therefore,age-match
R9445 T10727 T10729 aux did,age-match
R9446 T10728 T10729 neg not,age-match
R9447 T10729 T10729 ROOT age-match,age-match
R9448 T10730 T10732 det the,patients
R9449 T10731 T10732 compound CF,patients
R9450 T10732 T10729 dobj patients,age-match
R9451 T10733 T10732 cc and,patients
R9452 T10734 T10735 compound control,subjects
R9453 T10735 T10732 conj subjects,patients
R9454 T10736 T10729 punct .,age-match
R9455 T10737 T10738 amod Peripheral,blood
R9456 T10738 T10740 nsubjpass blood,collected
R9457 T10739 T10740 auxpass was,collected
R9458 T10740 T10740 ROOT collected,collected
R9459 T10741 T10740 prep in,collected
R9460 T10742 T10743 compound sodium,heparin
R9461 T10743 T10744 compound heparin,tubes
R9462 T10744 T10741 pobj tubes,in
R9463 T10745 T10747 punct (,Biosciences
R9464 T10746 T10747 compound BD,Biosciences
R9465 T10747 T10744 appos Biosciences,tubes
R9466 T10748 T10747 punct ),Biosciences
R9467 T10749 T10747 cc and,Biosciences
R9468 T10750 T10747 conj PBMCs,Biosciences
R9469 T10751 T10740 auxpass were,collected
R9470 T10752 T10751 acomp isolated,were
R9546 T10828 T10829 auxpass were,allowed
R9547 T10829 T10829 ROOT allowed,allowed
R9548 T10830 T10831 aux to,differentiate
R9549 T10831 T10829 xcomp differentiate,allowed
R9550 T10832 T10831 prep in,differentiate
R9551 T10833 T10834 compound RPMI,1640
R9552 T10834 T10832 pobj 1640,in
R9553 T10835 T10829 conj supplemented,allowed
R9554 T10836 T10835 prep with,supplemented
R9555 T10837 T10838 nummod 10,%
R9556 T10838 T10841 compound %,serum
R9557 T10839 T10840 compound human,AB
R9558 T10840 T10841 compound AB,serum
R9559 T10841 T10836 pobj serum,with
R9560 T10842 T10835 prep for,supplemented
R9561 T10843 T10844 nummod 10,days
R9562 T10844 T10842 pobj days,for
R9563 T10845 T10829 punct .,allowed
R9818 T11117 T11115 xcomp adhere,allowed
R9805 T11104 T11105 compound Cell,stimulation
R9806 T11105 T11113 nsubjpass stimulation,plated
R9807 T11106 T11105 cc and,stimulation
R9808 T11107 T11111 nmod cytokine,cells
R9809 T11108 T11111 nmod quantification,cells
R9810 T11109 T11111 nmod Bronchial,cells
R9811 T11110 T11111 amod epithelial,cells
R9812 T11111 T11105 conj cells,stimulation
R9813 T11112 T11113 auxpass were,plated
R9814 T11113 T11113 ROOT plated,plated
R9815 T11114 T11113 cc and,plated
R9816 T11115 T11113 conj allowed,plated
R9817 T11116 T11117 aux to,adhere
R9819 T11118 T11119 advmod overnight,prior
R9820 T11119 T11117 advmod prior,adhere
R9821 T11120 T11119 prep to,prior
R9822 T11121 T11120 pobj stimulation,to
R9823 T11122 T11113 punct .,plated
R9824 T11123 T11125 amod Bronchial,cells
R9825 T11124 T11125 amod epithelial,cells
R9826 T11125 T11127 nsubjpass cells,rested
R9827 T11126 T11127 auxpass were,rested
R9828 T11127 T11127 ROOT rested,rested
R9829 T11128 T11127 cc or,rested
R9830 T11129 T11127 conj primed,rested
R9831 T11130 T11129 prep with,primed
R9832 T11131 T11130 pobj LPS,with
R9833 T11132 T11129 prep for,primed
R9834 T11133 T11134 nummod 5,hours
R9835 T11134 T11132 pobj hours,for
R9836 T11135 T11129 cc and,primed
R9837 T11136 T11129 conj stimulated,primed
R9838 T11137 T11136 prep with,stimulated
R9839 T11138 T11141 amod live,PAO1
R9840 T11139 T11141 compound P.,PAO1
R9841 T11140 T11141 compound aeruginosa,PAO1
R9842 T11141 T11137 pobj PAO1,with
R9843 T11142 T11141 cc or,PAO1
R9844 T11143 T11141 conj ATP,PAO1
R9845 T11144 T11136 prep for,stimulated
R9846 T11145 T11146 det the,times
R9847 T11146 T11144 pobj times,for
R9848 T11147 T11127 conj indicated,rested
R9849 T11148 T11127 punct .,rested
R9850 T11149 T11156 nsubjpass PBMCs,rested
R9851 T11150 T11149 cc and,PBMCs
R9852 T11151 T11153 nummod THP-1,cells
R9853 T11152 T11153 compound reporter,cells
R9854 T11153 T11149 conj cells,PBMCs
R9855 T11154 T11156 auxpass were,rested
R9856 T11155 T11156 preconj either,rested
R9857 T11156 T11156 ROOT rested,rested
R9858 T11157 T11156 cc or,rested
R9859 T11158 T11156 conj primed,rested
R9860 T11159 T11158 prep with,primed
R9540 T10822 T10823 amod non-adherent,cells
R9541 T10823 T10825 nsubjpass cells,removed
R9542 T10824 T10825 auxpass were,removed
R9543 T10825 T10809 advcl removed,allowed
R9544 T10826 T10809 punct .,allowed
R9545 T10827 T10829 nsubjpass Monocytes,allowed
R9912 T11211 T11179 punct .,challenged
R9913 T11212 T11225 prep For,used
R9914 T11213 T11212 pobj stimulations,For
R9915 T11214 T11213 prep with,stimulations
R9916 T11215 T11214 pobj Poly,with
R9917 T11216 T11215 punct (,Poly
R9918 T11217 T11215 appos dA,Poly
R9919 T11218 T11215 punct :,Poly
R9920 T11219 T11215 appos dT,Poly
R9921 T11220 T11215 punct ),Poly
R9922 T11221 T11225 punct ",",used
R9923 T11222 T11223 compound lipofectamine,LTX
R9924 T11223 T11225 nsubjpass LTX,used
R9925 T11224 T11225 auxpass was,used
R9926 T11225 T11225 ROOT used,used
R9927 T11226 T11225 prep at,used
R9928 T11227 T11234 det a,v
R9929 T11228 T11234 nummod 1,v
R9930 T11229 T11234 nmod ∶,v
R9931 T11230 T11229 nummod 1,∶
R9932 T11231 T11234 punct (,v
R9933 T11232 T11234 nmod w,v
R9934 T11233 T11234 nmod ∶,v
R9935 T11234 T11226 pobj v,at
R9936 T11235 T11234 punct ),v
R9937 T11236 T11225 dobj ratio,used
R9938 T11237 T11236 prep of,ratio
R9939 T11238 T11237 pobj µg,of
R9940 T11239 T11238 prep of,µg
R9941 T11240 T11239 pobj DNA,of
R9942 T11241 T11225 prep to,used
R9943 T11242 T11241 pobj µl,to
R9944 T11243 T11242 prep of,µl
R9945 T11244 T11243 pobj lipofectamine,of
R9946 T11245 T11225 cc and,used
R9947 T11246 T11225 conj was,used
R9948 T11247 T11246 acomp mixed,was
R9949 T11248 T11249 nummod 30,minutes
R9950 T11249 T11250 npadvmod minutes,prior
R9951 T11250 T11246 advmod prior,was
R9952 T11251 T11250 prep to,prior
R9953 T11252 T11251 pobj stimulation,to
R9954 T11253 T11225 punct .,used
R9955 T11254 T11257 det The,Bay11-7082
R9956 T11255 T11257 amod NF-κB,Bay11-7082
R9957 T11256 T11257 compound inhibitor,Bay11-7082
R9958 T11257 T11262 nsubjpass Bay11-7082,added
R9959 T11258 T11259 punct (,Invivogen
R9960 T11259 T11257 appos Invivogen,Bay11-7082
R9961 T11260 T11259 punct ),Invivogen
R9962 T11261 T11262 auxpass was,added
R9963 T11262 T11262 ROOT added,added
R9964 T11263 T11262 prep to,added
R9965 T11264 T11263 pobj cultures,to
R9966 T11265 T11266 nummod 1,hour
R9967 T11266 T11267 npadvmod hour,prior
R9968 T11267 T11262 advmod prior,added
R9969 T11268 T11267 prep to,prior
R9970 T11269 T11268 pobj priming,to
R9971 T11270 T11262 punct .,added
R9972 T11271 T11275 mark If,involved
R9973 T11272 T11273 det no,priming
R9974 T11273 T11275 nsubjpass priming,involved
R9975 T11274 T11275 auxpass was,involved
R9976 T11275 T11279 advcl involved,added
R9977 T11276 T11279 punct ",",added
R9978 T11277 T11279 nsubjpass inhibitor,added
R9979 T11278 T11279 auxpass was,added
R9980 T11279 T11279 ROOT added,added
R9981 T11280 T11281 nummod 1,hour
R9982 T11281 T11282 npadvmod hour,prior
R9983 T11282 T11279 advmod prior,added
R9861 T11160 T11159 pobj LPS,with
R9862 T11161 T11160 punct (,LPS
R9863 T11162 T11160 appos Invivogen,LPS
R9864 T11163 T11160 punct ),LPS
R9865 T11164 T11158 cc or,primed
R9866 T11165 T11167 amod heat-killed,overnight
R9867 T11166 T11167 compound PAO1,overnight
R9868 T11167 T11158 conj overnight,primed
R9869 T11168 T11167 punct (,overnight
R9870 T11169 T11170 nummod 16,hours
R9871 T11170 T11167 appos hours,overnight
R9872 T11171 T11167 punct ),overnight
R9873 T11172 T11156 punct .,rested
R9874 T11173 T11175 det The,day
R9875 T11174 T11175 amod next,day
R9876 T11175 T11179 npadvmod day,challenged
R9877 T11176 T11177 det the,cells
R9878 T11177 T11179 nsubjpass cells,challenged
R9879 T11178 T11179 auxpass were,challenged
R9880 T11179 T11179 ROOT challenged,challenged
R9881 T11180 T11179 prep with,challenged
R9882 T11181 T11182 amod live,PAO1
R9883 T11182 T11180 pobj PAO1,with
R9884 T11183 T11182 punct ",",PAO1
R9885 T11184 T11182 conj PAO1ΔexsA,PAO1
R9886 T11185 T11184 punct ",",PAO1ΔexsA
R9887 T11186 T11184 conj ATP,PAO1ΔexsA
R9888 T11187 T11188 punct (,Invivogen
R9889 T11188 T11186 appos Invivogen,ATP
R9890 T11189 T11188 punct ),Invivogen
R9891 T11190 T11186 punct ",",ATP
R9892 T11191 T11186 cc or,ATP
R9893 T11192 T11186 conj Poly,ATP
R9894 T11193 T11192 punct (,Poly
R9895 T11194 T11192 appos dA,Poly
R9896 T11195 T11194 punct :,dA
R9897 T11196 T11194 nmod dT,dA
R9898 T11197 T11194 punct ),dA
R9899 T11198 T11200 punct (,Aldrich
R9900 T11199 T11200 compound Sigma,Aldrich
R9901 T11200 T11194 appos Aldrich,dA
R9902 T11201 T11192 punct ),Poly
R9903 T11202 T11179 prep for,challenged
R9904 T11203 T11204 det the,times
R9905 T11204 T11202 pobj times,for
R9906 T11205 T11179 conj indicated,challenged
R9907 T11206 T11207 punct (,see
R9908 T11207 T11205 parataxis see,indicated
R9909 T11208 T11207 dobj Fig.,see
R9910 T11209 T11208 nummod 1,Fig.
R9911 T11210 T11207 punct ),see
R9986 T11285 T11283 pobj stimulation,to
R9987 T11286 T11279 punct .,added
R9988 T11287 T11289 det The,inhibitor
R9989 T11288 T11289 amod CFTR,inhibitor
R9990 T11289 T11296 nsubjpass inhibitor,added
R9991 T11290 T11289 appos CFTRinh172,inhibitor
R9992 T11291 T11293 punct (,Aldrich
R9993 T11292 T11293 compound Sigma,Aldrich
R9994 T11293 T11290 appos Aldrich,CFTRinh172
R9995 T11294 T11290 punct ),CFTRinh172
R9996 T11295 T11296 auxpass was,added
R9997 T11296 T11296 ROOT added,added
R9998 T11297 T11296 prep to,added
R9999 T11298 T11297 pobj cultures,to
R10000 T11299 T11300 nummod 18,hours
R10001 T11300 T11301 npadvmod hours,prior
R10002 T11301 T11296 advmod prior,added
R10003 T11302 T11301 prep to,prior
R10004 T11303 T11304 amod inflammasome,stimulation
R10005 T11304 T11302 pobj stimulation,to
R10006 T11305 T11296 punct .,added
R10007 T11306 T11308 det The,inhibitor
R10008 T11307 T11308 amod caspase-1,inhibitor
R10009 T11308 T11314 nsubjpass inhibitor,added
R10010 T11309 T11308 appos z-YVAD-fmk,inhibitor
R10011 T11310 T11311 punct (,Biovision
R10012 T11311 T11309 appos Biovision,z-YVAD-fmk
R10013 T11312 T11309 punct ),z-YVAD-fmk
R10014 T11313 T11314 auxpass was,added
R10015 T11314 T11314 ROOT added,added
R10016 T11315 T11314 prep to,added
R10017 T11316 T11315 pobj cultures,to
R10018 T11317 T11318 nummod 1,hour
R10019 T11318 T11319 npadvmod hour,prior
R10020 T11319 T11314 advmod prior,added
R10021 T11320 T11319 prep to,prior
R10022 T11321 T11322 amod inflammasome,stimulation
R10023 T11322 T11320 pobj stimulation,to
R10024 T11323 T11314 punct .,added
R10025 T11324 T11326 nsubjpass Supernatants,collected
R10026 T11325 T11326 auxpass were,collected
R10027 T11326 T11326 ROOT collected,collected
R10028 T11327 T11326 cc and,collected
R10029 T11328 T11326 conj stored,collected
R10030 T11329 T11328 prep at,stored
R10031 T11330 T11334 nummod −,Cytokines
R10032 T11331 T11332 nummod 20,°
R10033 T11332 T11334 compound °,Cytokines
R10034 T11333 T11334 compound C.,Cytokines
R10035 T11334 T11329 pobj Cytokines,at
R10036 T11335 T11326 conj released,collected
R10037 T11336 T11335 prep into,released
R10038 T11337 T11336 pobj supernatants,into
R10039 T11338 T11337 prep from,supernatants
R10040 T11339 T11338 pobj PBMCs,from
R10041 T11340 T11345 nsubjpass stimulated,quantified
R10042 T11341 T11340 prep with,stimulated
R10043 T11342 T11343 amod inflammasome,activators
R10044 T11343 T11341 pobj activators,with
R10045 T11344 T11345 auxpass were,quantified
R10046 T11345 T11326 conj quantified,collected
R10047 T11346 T11345 advcl using,quantified
R10048 T11347 T11348 compound sandwich,ELISA
R10049 T11348 T11346 dobj ELISA,using
R10050 T11349 T11348 punct (,ELISA
R10051 T11350 T11348 appos eBioscience,ELISA
R10052 T11351 T11348 punct ),ELISA
R10053 T11352 T11345 punct .,quantified
R10220 T11553 T11559 advcl Immunoblotting,seeded
R10221 T11554 T11557 nummod 1,cells
R10222 T11555 T11557 nummod ×,cells
R10223 T11556 T11557 nummod 106,cells
R10224 T11557 T11559 nsubjpass cells,seeded
R10225 T11558 T11559 auxpass were,seeded
R10226 T11559 T11559 ROOT seeded,seeded
R10227 T11560 T11559 prep in,seeded
R10228 T11561 T11562 amod 12-well,plates
R10229 T11562 T11560 pobj plates,in
R10230 T11563 T11559 punct ",",seeded
R10231 T11564 T11559 conj stimulated,seeded
R10232 T11565 T11566 mark as,indicated
R10233 T11566 T11564 advcl indicated,stimulated
R10234 T11567 T11564 punct ",",stimulated
R10235 T11568 T11564 cc and,stimulated
R10236 T11569 T11564 conj lysed,stimulated
R10237 T11570 T11569 prep in,lysed
R10238 T11571 T11572 compound RIPA,buffer
R10239 T11572 T11570 pobj buffer,in
R10240 T11573 T11569 dep supplemented,lysed
R10241 T11574 T11573 prep with,supplemented
R10242 T11575 T11576 compound Halt,protease
R10243 T11576 T11574 pobj protease,with
R10244 T11577 T11576 cc and,protease
R10245 T11578 T11580 compound phosphatase,cocktail
R10246 T11579 T11580 compound inhibitor,cocktail
R10247 T11580 T11576 conj cocktail,protease
R10248 T11581 T11583 punct (,Scientific
R10249 T11582 T11583 compound Thermo,Scientific
R10250 T11583 T11580 appos Scientific,cocktail
R10251 T11584 T11583 punct ),Scientific
R10252 T11585 T11559 punct .,seeded
R10253 T11586 T11587 compound Protein,concentrations
R10254 T11587 T11589 nsubjpass concentrations,determined
R10255 T11588 T11589 auxpass were,determined
R10256 T11589 T11589 ROOT determined,determined
R10257 T11590 T11589 agent by,determined
R9984 T11283 T11279 prep to,added
R9985 T11284 T11285 amod inflammasome,stimulation
R10329 T11662 T11663 compound IRDye,®
R10330 T11663 T11656 punct ®,probed
R10331 T11664 T11663 nummod 680,®
R10332 T11665 T11664 cc or,680
R10333 T11666 T11663 appos 800CW,®
R10334 T11667 T11669 punct (,Biosciences
R10335 T11668 T11669 compound LI-COR,Biosciences
R10336 T11669 T11666 appos Biosciences,800CW
R10337 T11670 T11663 punct ),®
R10338 T11671 T11656 prep for,probed
R10339 T11672 T11673 nummod 1,hour
R10340 T11673 T11671 pobj hour,for
R10341 T11674 T11656 punct .,probed
R10342 T11675 T11676 det Both,blocking
R10343 T11676 T11681 nsubjpass blocking,carried
R10344 T11677 T11676 cc and,blocking
R10345 T11678 T11676 conj probing,blocking
R10346 T11679 T11681 nsubjpass steps,carried
R10347 T11680 T11681 auxpass were,carried
R10348 T11681 T11681 ROOT carried,carried
R10349 T11682 T11681 prt out,carried
R10350 T11683 T11681 prep in,carried
R10351 T11684 T11685 amod tris-buffered,saline
R10352 T11685 T11683 pobj saline,in
R10353 T11686 T11688 punct (,Biosciences
R10354 T11687 T11688 compound G,Biosciences
R10355 T11688 T11685 appos Biosciences,saline
R10356 T11689 T11688 punct ),Biosciences
R10357 T11690 T11681 prep containing,carried
R10358 T11691 T11692 nummod 5,%
R10359 T11692 T11695 compound %,albumin
R10360 T11693 T11694 amod bovine,serum
R10361 T11694 T11695 compound serum,albumin
R10362 T11695 T11690 dobj albumin,containing
R10363 T11696 T11695 cc and,albumin
R10364 T11697 T11698 nummod 0.1,%
R10365 T11698 T11700 quantmod %,20
R10366 T11699 T11700 quantmod TWEEN,20
R10367 T11700 T11695 conj 20,albumin
R10563 T11929 T11937 nsubjpass Quantification,plated
R10564 T11930 T11929 prep of,Quantification
R10565 T11931 T11932 amod caspase-1,activity
R10566 T11932 T11930 pobj activity,of
R10567 T11933 T11935 amod Bronchial,cells
R10568 T11934 T11935 amod epithelial,cells
R10569 T11935 T11937 nsubjpass cells,plated
R10570 T11936 T11937 auxpass were,plated
R10571 T11937 T11937 ROOT plated,plated
R10572 T11938 T11937 prep in,plated
R10573 T11939 T11940 amod 6-well,plates
R10574 T11940 T11938 pobj plates,in
R10575 T11941 T11937 prep at,plated
R10576 T11942 T11946 nummod 5,overnight
R10577 T11943 T11946 nummod ×,overnight
R10578 T11944 T11945 nummod 105,cells/well
R10579 T11945 T11946 compound cells/well,overnight
R10580 T11946 T11941 pobj overnight,at
R10581 T11947 T11937 punct .,plated
R10582 T11948 T11950 nsubjpass Cells,primed
R10583 T11949 T11950 auxpass were,primed
R10584 T11950 T11950 ROOT primed,primed
R10585 T11951 T11950 prep with,primed
R10586 T11952 T11951 pobj LPS,with
R10587 T11953 T11950 prep for,primed
R10588 T11954 T11955 nummod 5,hours
R10589 T11955 T11953 pobj hours,for
R10590 T11956 T11950 cc and,primed
R10591 T11957 T11950 conj stimulated,primed
R10592 T11958 T11957 prep with,stimulated
R10593 T11959 T11958 pobj ATP,with
R10594 T11960 T11957 prep for,stimulated
R10595 T11961 T11962 nummod 1,hour
R10596 T11962 T11960 pobj hour,for
R10597 T11963 T11957 cc or,stimulated
R10598 T11964 T11957 conj stimulated,stimulated
R10599 T11965 T11964 prep with,stimulated
R10600 T11966 T11967 amod live,PAO1
R10601 T11967 T11965 pobj PAO1,with
R10602 T11968 T11964 prep for,stimulated
R10603 T11969 T11970 nummod 3,hours
R10258 T11591 T11592 compound Bradford,assay
R10259 T11592 T11590 pobj assay,by
R10260 T11593 T11595 punct (,Scientific
R10261 T11594 T11595 compound Thermo,Scientific
R10262 T11595 T11592 appos Scientific,assay
R10263 T11596 T11595 punct ),Scientific
R10264 T11597 T11589 punct .,determined
R10265 T11598 T11600 nsubjpass Lysates,resolved
R10266 T11599 T11600 auxpass were,resolved
R10267 T11600 T11600 ROOT resolved,resolved
R10268 T11601 T11600 agent by,resolved
R10269 T11602 T11601 pobj electrophoreses,by
R10270 T11603 T11602 prep on,electrophoreses
R10271 T11604 T11605 nummod 10,%
R10272 T11605 T11607 nmod %,gels
R10273 T11606 T11607 amod SDS-polyacrylamide,gels
R10274 T11607 T11603 pobj gels,on
R10275 T11608 T11600 cc and,resolved
R10276 T11609 T11600 conj transferred,resolved
R10277 T11610 T11609 prep onto,transferred
R10278 T11611 T11612 compound PVDF,membranes
R10279 T11612 T11610 pobj membranes,onto
R10280 T11613 T11614 punct (,Millipore
R10281 T11614 T11612 appos Millipore,membranes
R10282 T11615 T11612 punct ),membranes
R10283 T11616 T11600 punct .,resolved
R10284 T11617 T11619 nsubjpass Blots,blocked
R10285 T11618 T11619 auxpass were,blocked
R10286 T11619 T11619 ROOT blocked,blocked
R10287 T11620 T11619 prep for,blocked
R10288 T11621 T11622 nummod 1,hour
R10289 T11622 T11620 pobj hour,for
R10290 T11623 T11619 prep at,blocked
R10291 T11624 T11625 compound room,temperature
R10292 T11625 T11623 pobj temperature,at
R10293 T11626 T11619 cc and,blocked
R10294 T11627 T11619 conj probed,blocked
R10295 T11628 T11627 dobj overnight,probed
R10296 T11629 T11627 prep at,probed
R10297 T11630 T11631 nummod 4,°
R10298 T11631 T11629 pobj °,at
R10299 T11632 T11627 conj C,probed
R10300 T11633 T11632 prep for,C
R10301 T11634 T11633 pobj pro-IL-1β,for
R10302 T11635 T11637 punct (,Cruz
R10303 T11636 T11637 compound Santa,Cruz
R10304 T11637 T11634 appos Cruz,pro-IL-1β
R10305 T11638 T11637 punct ),Cruz
R10306 T11639 T11634 punct ",",pro-IL-1β
R10307 T11640 T11634 conj IκBα,pro-IL-1β
R10308 T11641 T11643 punct (,Signaling
R10309 T11642 T11643 compound Cell,Signaling
R10310 T11643 T11640 parataxis Signaling,IκBα
R10311 T11644 T11643 punct ),Signaling
R10312 T11645 T11643 punct ",",Signaling
R10313 T11646 T11640 cc or,IκBα
R10314 T11647 T11640 conj β-actin,IκBα
R10315 T11648 T11650 punct (,Signaling
R10316 T11649 T11650 compound Cell,Signaling
R10317 T11650 T11647 appos Signaling,β-actin
R10318 T11651 T11647 punct ),β-actin
R10319 T11652 T11619 punct .,blocked
R10320 T11653 T11656 nsubjpass Blots,probed
R10321 T11654 T11656 auxpass were,probed
R10322 T11655 T11656 advmod subsequently,probed
R10323 T11656 T11656 ROOT probed,probed
R10324 T11657 T11656 prep with,probed
R10325 T11658 T11660 amod fluorescently-labeled,antibodies
R10326 T11659 T11660 amod secondary,antibodies
R10327 T11660 T11657 pobj antibodies,with
R10328 T11661 T11656 punct ",",probed
R10368 T11701 T11700 punct (,20
R10369 T11702 T11700 quantmod Calbiochem,20
R10370 T11703 T11700 punct ),20
R10371 T11704 T11681 punct .,carried
R10372 T11705 T11707 nsubjpass Blots,imaged
R10373 T11706 T11707 auxpass were,imaged
R10374 T11707 T11707 ROOT imaged,imaged
R10375 T11708 T11707 prep on,imaged
R10376 T11709 T11714 det a,system
R10377 T11710 T11711 compound LI-COR,Odyssey
R10378 T11711 T11714 nmod Odyssey,system
R10379 T11712 T11714 amod infrared,system
R10380 T11713 T11714 compound imaging,system
R10381 T11714 T11708 pobj system,on
R10382 T11715 T11714 punct (,system
R10383 T11716 T11717 compound LI-COR,Biosciences
R10384 T11717 T11714 appos Biosciences,system
R10385 T11718 T11714 punct ),system
R10386 T11719 T11707 cc and,imaged
R10387 T11720 T11707 conj quantified,imaged
R10388 T11721 T11720 xcomp using,quantified
R10389 T11722 T11725 det the,software
R10390 T11723 T11725 amod included,software
R10391 T11724 T11725 compound analysis,software
R10392 T11725 T11721 dobj software,using
R10393 T11726 T11707 punct .,imaged
R10628 T11994 T11996 nmod ×,cells/well
R10629 T11995 T11996 nummod 105,cells/well
R10630 T11996 T11992 pobj cells/well,of
R10631 T11997 T11996 punct (,cells/well
R10632 T11998 T12001 nummod 2.5,cells/ml
R10633 T11999 T12001 nummod ×,cells/ml
R10634 T12000 T12001 nummod 106,cells/ml
R10635 T12001 T11996 appos cells/ml,cells/well
R10636 T12002 T11996 punct ),cells/well
R10637 T12003 T11996 punct ",",cells/well
R10638 T12004 T11991 acl primed,density
R10639 T12005 T12004 prep with,primed
R10640 T12006 T12005 pobj LPS,with
R10641 T12007 T12004 prep for,primed
R10642 T12008 T12009 nummod 5,hours
R10643 T12009 T12007 pobj hours,for
R10644 T12010 T11984 cc and,seeded
R10645 T12011 T12012 advmod then,stimulated
R10646 T12012 T11984 conj stimulated,seeded
R10647 T12013 T12012 prep with,stimulated
R10648 T12014 T12013 pobj ATP,with
R10649 T12015 T12012 prep for,stimulated
R10650 T12016 T12017 nummod 1,hour
R10651 T12017 T12015 pobj hour,for
R10652 T12018 T12017 cc or,hour
R10653 T12019 T12017 conj Poly,hour
R10654 T12020 T12019 punct (,Poly
R10655 T12021 T12019 nmod dA,Poly
R10656 T12022 T12019 punct :,Poly
R10657 T12023 T12019 appos dT,Poly
R10658 T12024 T12019 punct ),Poly
R10659 T12025 T12012 prep for,stimulated
R10660 T12026 T12027 nummod 3,hours
R10661 T12027 T12025 pobj hours,for
R10662 T12028 T12012 cc or,stimulated
R10663 T12029 T12012 conj stimulated,stimulated
R10664 T12030 T12029 prep with,stimulated
R10665 T12031 T12032 amod live,PAO1
R10666 T12032 T12030 pobj PAO1,with
R10667 T12033 T12029 prep for,stimulated
R10668 T12034 T12035 nummod 3,hours
R10669 T12035 T12033 pobj hours,for
R10670 T12036 T11984 punct .,seeded
R10671 T12037 T12038 compound Caspase-1,activity
R10672 T12038 T12040 nsubjpass activity,measured
R10673 T12039 T12040 auxpass was,measured
R10674 T12040 T12040 ROOT measured,measured
R10675 T12041 T12040 advcl using,measured
R10676 T12042 T12041 dobj FLICA,using
R10677 T12043 T12045 punct (,Technologies
R10678 T12044 T12045 compound Immunochemistry,Technologies
R10679 T12045 T12042 appos Technologies,FLICA
R10680 T12046 T12045 punct ),Technologies
R10681 T12047 T12045 punct ",",Technologies
R10682 T12048 T12051 det a,probe
R10683 T12049 T12051 amod cell-permeable,probe
R10684 T12050 T12051 amod fluorescent,probe
R10685 T12051 T12045 appos probe,Technologies
R10686 T12052 T12051 punct (,probe
R10687 T12053 T12051 amod FAM-YVAD-fmk,probe
R10604 T11970 T11968 pobj hours,for
R10605 T11971 T11950 punct .,primed
R10606 T11972 T11974 nsubjpass PBMCs,stimulated
R10607 T11973 T11974 auxpass were,stimulated
R10608 T11974 T11974 ROOT stimulated,stimulated
R10609 T11975 T11977 det the,day
R10610 T11976 T11977 amod same,day
R10611 T11977 T11974 npadvmod day,stimulated
R10612 T11978 T11977 prep as,day
R10613 T11979 T11980 compound blood,donation
R10614 T11980 T11978 pobj donation,as
R10615 T11981 T11974 punct .,stimulated
R10616 T11982 T11984 nsubjpass PBMCs,seeded
R10617 T11983 T11984 auxpass were,seeded
R10618 T11984 T11984 ROOT seeded,seeded
R10619 T11985 T11984 prep in,seeded
R10620 T11986 T11988 det a,plate
R10621 T11987 T11988 amod 96-well,plate
R10622 T11988 T11985 pobj plate,in
R10623 T11989 T11984 prep at,seeded
R10624 T11990 T11991 det a,density
R10625 T11991 T11989 pobj density,at
R10626 T11992 T11991 prep of,density
R10627 T11993 T11996 nummod 4.5,cells/well
R10809 T12209 T12214 compound NF-κB,Supernatants
R10810 T12210 T12214 compound /,Supernatants
R10811 T12211 T12214 compound AP-1,Supernatants
R10812 T12212 T12214 compound Activity,Supernatants
R10813 T12213 T12214 compound Assay,Supernatants
R10814 T12214 T12220 nsubjpass Supernatants,incubated
R10815 T12215 T12214 prep from,Supernatants
R10816 T12216 T12218 nummod THP-1,cells
R10817 T12217 T12218 compound reporter,cells
R10818 T12218 T12215 pobj cells,from
R10819 T12219 T12220 auxpass were,incubated
R10820 T12220 T12220 ROOT incubated,incubated
R10821 T12221 T12220 prep with,incubated
R10822 T12222 T12223 amod Quanti-Blue,substrate
R10823 T12223 T12221 pobj substrate,with
R10824 T12224 T12225 punct (,Invivogen
R10825 T12225 T12223 appos Invivogen,substrate
R10826 T12226 T12225 punct ),Invivogen
R10827 T12227 T12220 prep at,incubated
R10828 T12228 T12229 nummod 37,°
R10829 T12229 T12230 nummod °,C
R10830 T12230 T12227 pobj C,at
R10831 T12231 T12220 cc and,incubated
R10832 T12232 T12220 conj allowed,incubated
R10833 T12233 T12234 aux to,develop
R10834 T12234 T12232 xcomp develop,allowed
R10835 T12235 T12234 prep for,develop
R10836 T12236 T12235 pobj 16,for
R10837 T12237 T12238 nummod 18,hours
R10838 T12238 T12236 appos hours,16
R10839 T12239 T12220 punct .,incubated
R10840 T12240 T12241 nsubj Quanti-Blue,contains
R10841 T12241 T12241 ROOT contains,contains
R10842 T12242 T12243 det a,substrate
R11066 T12482 T12471 appos streptomycin,cultures
R11067 T12483 T12487 punct (,ml
R11068 T12484 T12485 nummod 150,µg
R11069 T12485 T12487 nmod µg,ml
R11070 T12486 T12487 compound /,ml
R11071 T12487 T12482 appos ml,streptomycin
R11072 T12488 T12487 punct ),ml
R11073 T12489 T12469 prep until,from
R11074 T12490 T12491 amod mid-logarithmic,phase
R11075 T12491 T12489 pobj phase,until
R11076 T12492 T12468 punct .,grown
R11077 T12493 T12495 nsubjpass Cells,washed
R11078 T12494 T12495 auxpass were,washed
R11079 T12495 T12495 ROOT washed,washed
R11080 T12496 T12495 advmod once,washed
R11081 T12497 T12495 prep in,washed
R11082 T12498 T12497 pobj PBS,in
R11083 T12499 T12495 cc and,washed
R11084 T12500 T12495 conj resuspended,washed
R11085 T12501 T12500 prep in,resuspended
R11086 T12502 T12501 pobj PBS,in
R11087 T12503 T12500 prep to,resuspended
R11088 T12504 T12506 det an,density
R11089 T12505 T12506 amod optical,density
R11127 T12543 T12546 prep For,live
R11128 T12544 T12543 pobj stimulations,For
R11129 T12545 T12546 punct ",",live
R11130 T12546 T12546 ROOT live,live
R11131 T12547 T12549 nsubjpass PAO1,resuspended
R11132 T12548 T12549 auxpass was,resuspended
R11133 T12549 T12546 ccomp resuspended,live
R11134 T12550 T12549 prep to,resuspended
R10688 T12054 T12045 punct ),Technologies
R10689 T12055 T12056 nsubj that,binds
R10690 T12056 T12042 relcl binds,FLICA
R10691 T12057 T12058 amod active,caspase-1
R10692 T12058 T12056 dobj caspase-1,binds
R10693 T12059 T12040 punct .,measured
R10694 T12060 T12062 nsubjpass Cells,incubated
R10695 T12061 T12062 auxpass were,incubated
R10696 T12062 T12062 ROOT incubated,incubated
R10697 T12063 T12064 nummod 1,hour
R10698 T12064 T12065 npadvmod hour,with
R10699 T12065 T12062 prep with,incubated
R10700 T12066 T12065 pobj FLICA,with
R10701 T12067 T12062 prep at,incubated
R10702 T12068 T12069 nummod 37,°
R10703 T12069 T12070 nummod °,C
R10704 T12070 T12067 pobj C,at
R10705 T12071 T12062 cc and,incubated
R10706 T12072 T12062 conj stained,incubated
R10707 T12073 T12072 prep with,stained
R10708 T12074 T12076 amod PE-Cy7-conjugated,antibodies
R10709 T12075 T12076 amod anti-CD14,antibodies
R10710 T12076 T12073 pobj antibodies,with
R10711 T12077 T12078 punct (,eBioscience
R10712 T12078 T12076 appos eBioscience,antibodies
R10713 T12079 T12076 punct ),antibodies
R10714 T12080 T12081 aux to,identify
R10715 T12081 T12072 advcl identify,stained
R10716 T12082 T12081 dobj monocytes,identify
R10717 T12083 T12062 punct .,incubated
R10718 T12084 T12086 det The,strategy
R10719 T12085 T12086 amod gating,strategy
R10720 T12086 T12087 nsubj strategy,consisted
R10721 T12087 T12087 ROOT consisted,consisted
R10722 T12088 T12087 prep of,consisted
R10723 T12089 T12088 pcomp including,of
R10724 T12090 T12091 amod live,cells
R10725 T12091 T12089 pobj cells,including
R10726 T12092 T12093 nsubj that,were
R10727 T12093 T12091 relcl were,cells
R10728 T12094 T12093 attr CD14,were
R10729 T12095 T12094 amod positive,CD14
R10730 T12096 T12099 nsubjpass which,analyzed
R10731 T12097 T12099 auxpass were,analyzed
R10732 T12098 T12099 advmod subsequently,analyzed
R10733 T12099 T12095 relcl analyzed,positive
R10734 T12100 T12099 prep for,analyzed
R10735 T12101 T12102 det the,frequency
R10736 T12102 T12100 pobj frequency,for
R10737 T12103 T12102 prep of,frequency
R10738 T12104 T12106 amod FLICA,cells
R10739 T12105 T12106 amod positive,cells
R10740 T12106 T12103 pobj cells,of
R10741 T12107 T12087 punct .,consisted
R10843 T12243 T12241 dobj substrate,contains
R10844 T12244 T12243 prep for,substrate
R10845 T12245 T12246 amod alkaline,phosphatase
R10846 T12246 T12244 pobj phosphatase,for
R10847 T12247 T12246 cc and,phosphatase
R10848 T12248 T12258 nsubjpass changes,quantified
R10849 T12249 T12248 prep in,changes
R10850 T12250 T12251 det the,amount
R10851 T12251 T12249 pobj amount,in
R10852 T12252 T12251 prep of,amount
R10853 T12253 T12255 compound NF-κB,AP-1
R10854 T12254 T12255 compound /,AP-1
R10855 T12255 T12256 compound AP-1,activity
R10856 T12256 T12252 pobj activity,of
R10857 T12257 T12258 auxpass were,quantified
R10858 T12258 T12243 relcl quantified,substrate
R10859 T12259 T12258 agent by,quantified
R10860 T12260 T12261 amod optical,density
R10861 T12261 T12259 pobj density,by
R10862 T12262 T12265 punct (,655
R10863 T12263 T12265 nmod λ,655
R10864 T12264 T12265 punct =,655
R10865 T12265 T12261 appos 655,density
R10866 T12266 T12261 punct ),density
R10867 T12267 T12243 acl measured,substrate
R10868 T12268 T12267 advcl using,measured
R10869 T12269 T12274 det a,reader
R10870 T12270 T12272 nmod SpectraMax,Plus
R10871 T12271 T12272 nummod 384,Plus
R10872 T12272 T12274 nmod Plus,reader
R10873 T12273 T12274 compound plate,reader
R10874 T12274 T12268 dobj reader,using
R10875 T12275 T12274 cc and,reader
R10876 T12276 T12278 nmod SoftMax,software
R10877 T12277 T12278 amod Pro,software
R10878 T12278 T12274 conj software,reader
R10879 T12279 T12281 punct (,Devices
R10880 T12280 T12281 compound Molecular,Devices
R10881 T12281 T12278 appos Devices,software
R10882 T12282 T12241 punct ),contains
R10883 T12283 T12241 punct .,contains
R11027 T12443 T12444 amod Bacterial,strains
R11028 T12444 T12455 nsubjpass strains,obtained
R11029 T12445 T12455 npadvmod P.,obtained
R11030 T12446 T12448 nmod aeruginosa,strains
R11031 T12447 T12448 compound laboratory,strains
R11032 T12448 T12455 nsubjpass strains,obtained
R11033 T12449 T12448 appos PAO1,strains
R11034 T12450 T12449 cc and,PAO1
R11035 T12451 T12453 det the,mutant
R11036 T12452 T12453 compound PAO1ΔexsA,mutant
R11037 T12453 T12449 conj mutant,PAO1
R11038 T12454 T12455 auxpass were,obtained
R11039 T12455 T12455 ROOT obtained,obtained
R11040 T12456 T12455 prep from,obtained
R11041 T12457 T12459 compound Dr.,Hancock
R11042 T12458 T12459 compound Robert,Hancock
R11043 T12459 T12456 pobj Hancock,from
R11044 T12460 T12455 punct .,obtained
R11045 T12461 T12468 nsubjpass P.,grown
R11046 T12462 T12463 compound aeruginosa,strains
R11047 T12463 T12468 nsubjpass strains,grown
R11048 T12464 T12463 appos PAO1,strains
R11049 T12465 T12464 cc and,PAO1
R11050 T12466 T12464 conj PAO1ΔexsA,PAO1
R11051 T12467 T12468 auxpass were,grown
R11052 T12468 T12468 ROOT grown,grown
R11053 T12469 T12468 prep from,grown
R11054 T12470 T12471 amod overnight,cultures
R11055 T12471 T12469 pobj cultures,from
R11056 T12472 T12471 prep in,cultures
R11057 T12473 T12474 compound Luria,Bertani
R11058 T12474 T12472 pobj Bertani,in
R11059 T12475 T12474 punct (,Bertani
R11060 T12476 T12474 appos LB,Bertani
R11061 T12477 T12474 punct ),Bertani
R11062 T12478 T12471 conj broth,cultures
R11063 T12479 T12478 cc and,broth
R11064 T12480 T12482 compound LB,streptomycin
R11065 T12481 T12482 compound +,streptomycin
R11090 T12506 T12503 pobj density,to
R11091 T12507 T12506 prep of,density
R11092 T12508 T12507 pobj 0.5,of
R11093 T12509 T12513 punct (,nm
R11094 T12510 T12512 dep λ,600
R11095 T12511 T12512 punct =,600
R11096 T12512 T12513 nummod 600,nm
R11097 T12513 T12506 appos nm,density
R11098 T12514 T12500 punct ),resuspended
R11099 T12515 T12495 punct .,washed
R11100 T12516 T12517 aux To,prepare
R11101 T12517 T12521 advcl prepare,live
R11102 T12518 T12519 amod heat-killed,bacteria
R11103 T12519 T12517 dobj bacteria,prepare
R11104 T12520 T12521 punct ",",live
R11105 T12521 T12521 ROOT live,live
R11106 T12522 T12524 nsubjpass PAO1,resuspended
R11107 T12523 T12524 auxpass was,resuspended
R11108 T12524 T12521 ccomp resuspended,live
R11109 T12525 T12524 prep in,resuspended
R11110 T12526 T12525 pobj PBS,in
R11111 T12527 T12524 prep to,resuspended
R11112 T12528 T12530 det an,density
R11135 T12551 T12553 det an,density
R11136 T12552 T12553 amod optical,density
R11137 T12553 T12550 pobj density,to
R11138 T12554 T12553 prep of,density
R11139 T12555 T12554 pobj 0.5,of
R11140 T12556 T12553 prep in,density
R11141 T12557 T12556 pobj PBS,in
R11142 T12558 T12549 cc and,resuspended
R11143 T12559 T12560 advmod further,diluted
R11144 T12560 T12549 conj diluted,resuspended
R11145 T12561 T12560 prep in,diluted
R11146 T12562 T12563 compound culture,medium
R11147 T12563 T12561 pobj medium,in
R11148 T12564 T12560 advmod prior,diluted
R11149 T12565 T12564 prep to,prior
R11150 T12566 T12565 pobj stimulation,to
R11151 T12567 T12568 aux to,achieve
R11152 T12568 T12560 advcl achieve,diluted
R11153 T12569 T12571 det the,multiplicity
R11154 T12570 T12571 amod desired,multiplicity
R11155 T12571 T12568 dobj multiplicity,achieve
R11156 T12572 T12571 prep of,multiplicity
R11157 T12573 T12572 pobj infection,of
R11158 T12574 T12546 punct .,live
R11159 T12575 T12576 compound Heat-killed,PAO1
R11160 T12576 T12578 nsubjpass PAO1,added
R11161 T12577 T12578 auxpass was,added
R11162 T12578 T12578 ROOT added,added
R11163 T12579 T12578 prep in,added
R11164 T12580 T12582 det a,equivalent
R11165 T12581 T12582 compound volume,equivalent
R11166 T12582 T12579 pobj equivalent,in
R11167 T12583 T12582 prep to,equivalent
R11168 T12584 T12585 det that,used
R11169 T12585 T12583 pobj used,to
R11170 T12586 T12587 aux to,achieve
R11171 T12587 T12585 xcomp achieve,used
R11172 T12588 T12589 det an,MOI
R11173 T12589 T12587 dobj MOI,achieve
R11174 T12590 T12589 prep of,MOI
R11175 T12591 T12590 pobj 1,of
R11176 T12592 T12587 prep for,achieve
R11177 T12593 T12594 amod live,PAO1
R11178 T12594 T12592 pobj PAO1,for
R11179 T12595 T12578 punct .,added
R11475 T12939 T12941 nmod Airway,cells
R11476 T12940 T12941 amod epithelial,cells
R11477 T12941 T12945 nsubj cells,contribute
R11478 T12942 T12945 aux do,contribute
R11479 T12943 T12945 neg not,contribute
R11480 T12944 T12945 advmod significantly,contribute
R11481 T12945 T12945 ROOT contribute,contribute
R11482 T12946 T12945 prep to,contribute
R11483 T12947 T12948 amod IL-1β,production
R11484 T12948 T12946 pobj production,to
R11485 T12949 T12945 prep in,contribute
R11486 T12950 T12949 pobj response,in
R11487 T12951 T12950 prep to,response
R11488 T12952 T12953 amod inflammasome,stimuli
R11489 T12953 T12951 pobj stimuli,to
R11490 T12954 T12945 punct .,contribute
R11491 T12955 T12957 compound Control,lines
R11492 T12956 T12957 compound cell,lines
R11493 T12957 T12989 nsubjpass lines,stimulated
R11494 T12958 T12957 punct (,lines
R11495 T12959 T12960 punct (,A
R11496 T12960 T12957 appos A,lines
R11497 T12961 T12960 punct ),A
R11498 T12962 T12960 appos S9,A
R11499 T12963 T12960 punct ",",A
R11500 T12964 T12965 punct (,C
R11501 T12965 T12960 appos C,A
R11502 T12966 T12965 punct ),C
R11503 T12967 T12960 conj NuLi-1,A
R11504 T12968 T12967 punct ),NuLi-1
R11505 T12969 T12957 cc and,lines
R11506 T12970 T12974 poss their,lines
R11507 T12971 T12974 amod corresponding,lines
R11508 T12972 T12973 compound CF,cell
R11509 T12973 T12974 compound cell,lines
R11510 T12974 T12957 conj lines,lines
R11578 T13042 T13043 auxpass were,assayed
R11579 T13043 T13043 ROOT assayed,assayed
R11580 T13044 T13043 prep for,assayed
R11581 T13045 T13048 amod IL-1β,production
R11582 T13046 T13045 cc and,IL-1β
R11583 T13047 T13045 conj IL-8,IL-1β
R11584 T13048 T13044 pobj production,for
R11585 T13049 T13048 prep by,production
R11586 T13050 T13049 pobj ELISA,by
R11587 T13051 T13043 punct .,assayed
R11588 T13052 T13052 ROOT Insert,Insert
R11589 T13053 T13052 dobj shows,Insert
R11590 T13054 T13055 amod IL-8,secretion
R11113 T12529 T12530 amod optical,density
R11114 T12530 T12527 pobj density,to
R11115 T12531 T12530 prep of,density
R11116 T12532 T12531 pobj 0.5,of
R11117 T12533 T12524 cc and,resuspended
R11118 T12534 T12524 conj heated,resuspended
R11119 T12535 T12534 prep at,heated
R11120 T12536 T12537 nummod 60,°
R11121 T12537 T12535 pobj °,at
R11122 T12538 T12524 conj C,resuspended
R11123 T12539 T12538 prep for,C
R11124 T12540 T12541 nummod 1,hour
R11125 T12541 T12539 pobj hour,for
R11126 T12542 T12521 punct .,live
R11213 T12642 T12645 nsubj Statistics,display
R11214 T12643 T12644 det All,graphs
R11215 T12644 T12645 nsubj graphs,display
R11216 T12645 T12645 ROOT display,display
R11217 T12646 T12649 det the,SEM
R11218 T12647 T12649 amod mean,SEM
R11219 T12648 T12649 compound ±,SEM
R11220 T12649 T12645 dobj SEM,display
R11221 T12650 T12645 cc and,display
R11222 T12651 T12652 auxpass were,generated
R11223 T12652 T12645 conj generated,display
R11224 T12653 T12652 prep with,generated
R11225 T12654 T12653 pobj Prism,with
R11226 T12655 T12654 nummod 5,Prism
R11227 T12656 T12657 punct (,Graphpad
R11228 T12657 T12654 appos Graphpad,Prism
R11229 T12658 T12654 punct ),Prism
R11230 T12659 T12645 punct .,display
R11231 T12660 T12661 compound Statistical,significance
R11232 T12661 T12663 nsubjpass significance,determined
R11233 T12662 T12663 auxpass was,determined
R11234 T12663 T12663 ROOT determined,determined
R11235 T12664 T12663 prep by,determined
R11236 T12665 T12664 pcomp performing,by
R11237 T12666 T12665 dobj one,performing
R11238 T12667 T12666 cc or,one
R11239 T12668 T12669 amod two-way,ANOVA
R11240 T12669 T12666 conj ANOVA,one
R11241 T12670 T12669 cc and,ANOVA
R11242 T12671 T12672 det the,Bonferroni
R11243 T12672 T12669 conj Bonferroni,ANOVA
R11244 T12673 T12675 advmod post-test,applicable
R11245 T12674 T12675 advmod where,applicable
R11246 T12675 T12672 relcl applicable,Bonferroni
R11247 T12676 T12663 punct .,determined
R11292 T12728 T12729 compound Cell,stimulation
R11293 T12729 T12729 ROOT stimulation,stimulation
R11294 T12730 T12729 cc and,stimulation
R11295 T12731 T12732 compound inhibitor,schedule
R11296 T12732 T12729 conj schedule,stimulation
R11297 T12733 T12729 punct .,stimulation
R11298 T12734 T12735 nsubj Schedule,outlines
R11299 T12735 T12735 ROOT outlines,outlines
R11300 T12736 T12737 det the,timing
R11301 T12737 T12735 dobj timing,outlines
R11302 T12738 T12737 prep of,timing
R11303 T12739 T12740 compound inhibitor,addition
R11304 T12740 T12738 pobj addition,of
R11305 T12741 T12740 cc and,addition
R11306 T12742 T12738 pcomp priming,of
R11307 T12743 T12742 prep in,priming
R11308 T12744 T12743 pobj relation,in
R11309 T12745 T12744 prep to,relation
R11310 T12746 T12747 amod inflammasome,stimulation
R11311 T12747 T12745 pobj stimulation,to
R11312 T12748 T12747 punct (,stimulation
R11313 T12749 T12747 appos t,stimulation
R11314 T12750 T12751 punct =,0
R11315 T12751 T12749 prep 0,t
R11316 T12752 T12747 punct ),stimulation
R11317 T12753 T12735 prep for,outlines
R11318 T12754 T12755 nummod THP-1,reporter
R11319 T12755 T12753 pobj reporter,for
R11320 T12756 T12755 cc and,reporter
R11321 T12757 T12758 compound PBMC,cytokine
R11322 T12758 T12760 compound cytokine,experiments
R11323 T12759 T12760 compound quantification,experiments
R11324 T12760 T12755 conj experiments,reporter
R11325 T12761 T12735 punct .,outlines
R11326 T12762 T12763 compound Inhibitor,treatments
R11327 T12763 T12767 nsubjpass treatments,carried
R11328 T12764 T12763 cc and,treatments
R11329 T12765 T12763 conj stimulations,treatments
R11330 T12766 T12767 auxpass were,carried
R11331 T12767 T12767 ROOT carried,carried
R11332 T12768 T12767 prt out,carried
R11333 T12769 T12770 mark as,described
R11334 T12770 T12767 advcl described,carried
R11335 T12771 T12770 prep in,described
R11336 T12772 T12773 det the,Materials
R11337 T12773 T12771 pobj Materials,in
R11338 T12774 T12773 cc and,Materials
R11339 T12775 T12773 conj Methods,Materials
R11340 T12776 T12771 pobj section,in
R11341 T12777 T12767 punct .,carried
R11511 T12975 T12974 punct (,lines
R11512 T12976 T12979 punct (,IB3-1
R11513 T12977 T12979 nmod B,IB3-1
R11514 T12978 T12979 punct ),IB3-1
R11515 T12979 T12985 meta IB3-1,CuFi-1
R11516 T12980 T12979 punct ",",IB3-1
R11517 T12981 T12979 cc and,IB3-1
R11518 T12982 T12983 punct (,D
R11519 T12983 T12979 conj D,IB3-1
R11520 T12984 T12979 punct ),IB3-1
R11521 T12985 T12974 appos CuFi-1,lines
R11522 T12986 T12987 punct ),cells
R11523 T12987 T12989 nsubjpass cells,stimulated
R11524 T12988 T12989 auxpass were,stimulated
R11525 T12989 T12989 ROOT stimulated,stimulated
R11526 T12990 T12989 prep with,stimulated
R11527 T12991 T12992 compound P.,aeruginosa
R11528 T12992 T12990 pobj aeruginosa,with
R11529 T12993 T13002 punct (,mM
R11530 T12994 T13002 nmod MOI,mM
R11531 T12995 T12994 punct =,MOI
R11532 T12996 T12994 nummod 10,MOI
R11533 T12997 T12994 punct ),MOI
R11534 T12998 T12994 punct ",",MOI
R11535 T12999 T12994 conj ATP,MOI
R11536 T13000 T13001 punct (,5
R11537 T13001 T13002 nummod 5,mM
R11538 T13002 T12992 appos mM,aeruginosa
R11539 T13003 T13002 punct ),mM
R11540 T13004 T13002 punct ",",mM
R11541 T13005 T12992 cc or,aeruginosa
R11542 T13006 T12992 conj IL-1β,aeruginosa
R11543 T13007 T13006 punct (,IL-1β
R11544 T13008 T13009 nummod 10,ng/ml
R11545 T13009 T13006 appos ng/ml,IL-1β
R11546 T13010 T13006 punct ),IL-1β
R11547 T13011 T12989 punct ",",stimulated
R11548 T13012 T12989 prep for,stimulated
R11549 T13013 T13015 det the,times
R11550 T13014 T13015 amod indicated,times
R11551 T13015 T13012 pobj times,for
R11552 T13016 T13015 punct (,times
R11553 T13017 T13021 advmod n,experiments
R11554 T13018 T13021 punct =,experiments
R11555 T13019 T13021 nummod 3,experiments
R11556 T13020 T13021 amod individual,experiments
R11557 T13021 T13015 appos experiments,times
R11558 T13022 T13015 punct ),times
R11559 T13023 T12989 punct .,stimulated
R11560 T13024 T13026 nsubjpass Cells,primed
R11561 T13025 T13026 auxpass were,primed
R11562 T13026 T13026 ROOT primed,primed
R11563 T13027 T13026 prep with,primed
R11564 T13028 T13027 pobj LPS,with
R11565 T13029 T13031 punct (,ng/ml
R11566 T13030 T13031 nummod 100,ng/ml
R11567 T13031 T13028 appos ng/ml,LPS
R11568 T13032 T13026 punct ),primed
R11569 T13033 T13026 prep for,primed
R11591 T13055 T13052 npadvmod secretion,Insert
R11592 T13056 T13052 prep in,Insert
R11593 T13057 T13056 pobj response,in
R11594 T13058 T13057 prep to,response
R11595 T13059 T13058 pobj stimulation,to
R11596 T13060 T13057 prep with,response
R11597 T13061 T13060 pobj IL-1β,with
R11598 T13062 T13061 punct (,IL-1β
R11599 T13063 T13064 nummod 10,ng/ml
R11770 T13270 T13271 amod inflammasome,stimulation
R11771 T13271 T13269 pobj stimulation,following
R11772 T13272 T13271 prep with,stimulation
R11773 T13273 T13274 compound P.,aeruginosa
R11774 T13274 T13272 pobj aeruginosa,with
R11775 T13275 T13278 punct (,10
R11776 T13276 T13278 dep MOI,10
R11777 T13277 T13278 punct =,10
R11778 T13278 T13274 appos 10,aeruginosa
R11779 T13279 T13274 punct ),aeruginosa
R11780 T13280 T13274 cc and,aeruginosa
R11781 T13281 T13274 conj ATP,aeruginosa
R11782 T13282 T13283 punct (,5
R11783 T13283 T13284 nummod 5,mM
R11784 T13284 T13281 appos mM,ATP
R11785 T13285 T13281 punct ),ATP
R11786 T13286 T13265 punct .,examined
R11787 T13287 T13289 nsubjpass Cells,primed
R11788 T13288 T13289 auxpass were,primed
R11789 T13289 T13289 ROOT primed,primed
R11790 T13290 T13289 prep with,primed
R11791 T13291 T13290 pobj LPS,with
R11792 T13292 T13289 prep for,primed
R11793 T13293 T13294 nummod 5,hours
R11794 T13294 T13292 pobj hours,for
R11795 T13295 T13296 advmod where,appropriate
R11796 T13296 T13294 relcl appropriate,hours
R11797 T13297 T13289 punct .,primed
R11798 T13298 T13300 det A,histogram
R11799 T13299 T13300 amod representative,histogram
R11800 T13300 T13306 nsubjpass histogram,shown
R11801 T13301 T13300 prep of,histogram
R11802 T13302 T13304 nmod %,cells
R11803 T13303 T13304 amod caspase-1-active,cells
R11804 T13304 T13301 pobj cells,of
R11805 T13305 T13306 auxpass is,shown
R11806 T13306 T13306 ROOT shown,shown
R11807 T13307 T13306 prep in,shown
R11808 T13308 T13309 punct (,A
R11809 T13309 T13307 pobj A,in
R11810 T13310 T13306 punct ),shown
R11811 T13311 T13306 cc and,shown
R11812 T13312 T13314 det the,values
R11813 T13313 T13314 amod averaged,values
R11814 T13314 T13316 nsubjpass values,shown
R11815 T13315 T13316 auxpass are,shown
R11816 T13316 T13306 conj shown,shown
R11817 T13317 T13316 prep in,shown
R11818 T13318 T13319 punct (,B
R11819 T13319 T13317 pobj B,in
R11820 T13320 T13319 punct ),B
R11821 T13321 T13316 punct (,shown
R11822 T13322 T13326 advmod n,experiments
R11823 T13323 T13326 punct =,experiments
R11824 T13324 T13326 nummod 3,experiments
R11825 T13325 T13326 amod separate,experiments
R11826 T13326 T13316 appos experiments,shown
R11827 T13327 T13316 punct ),shown
R11828 T13328 T13316 punct .,shown
R11829 T13329 T13330 punct (,C
R11830 T13330 T13333 nmod C,cells
R11831 T13331 T13330 punct ),C
R11832 T13332 T13333 amod IB3-1,cells
R11833 T13333 T13340 nsubj cells,were
R11834 T13334 T13333 punct (,cells
R11835 T13335 T13338 nummod 5,cells/well
R12049 T13572 T13576 punct (,ml
R12050 T13573 T13574 nummod 1,µg
R12051 T13574 T13576 nmod µg,ml
R12052 T13575 T13576 compound /,ml
R12053 T13576 T13566 appos ml,Poly
R12054 T13577 T13543 punct ),primed
R12055 T13578 T13543 prep for,primed
R12056 T13579 T13580 nummod 3,hours
R12057 T13580 T13578 pobj hours,for
R12058 T13581 T13543 punct .,primed
R12059 T13582 T13584 nsubjpass PBMCs,stimulated
R12060 T13583 T13584 auxpass were,stimulated
R12061 T13584 T13584 ROOT stimulated,stimulated
R12062 T13585 T13584 prep with,stimulated
R12063 T13586 T13588 nsubj P.,strain
R12064 T13587 T13588 compound aeruginosa,strain
R12065 T13588 T13585 pobj strain,with
R12066 T13589 T13588 appos PAO1,strain
R12067 T13590 T13584 prep for,stimulated
R12068 T13591 T13592 nummod 3,hours
R12069 T13592 T13590 pobj hours,for
R12070 T13593 T13584 punct .,stimulated
R12071 T13594 T13596 det A,histogram
R12072 T13595 T13596 amod representative,histogram
R12073 T13596 T13603 nsubjpass histogram,shown
R12074 T13597 T13596 prep of,histogram
R11570 T13034 T13035 nummod 4,hours
R11571 T13035 T13033 pobj hours,for
R11572 T13036 T13037 advmod where,appropriate
R11573 T13037 T13035 relcl appropriate,hours
R11574 T13038 T13026 punct .,primed
R11575 T13039 T13040 compound Cell,culture
R11576 T13040 T13041 compound culture,supernatants
R11577 T13041 T13043 nsubjpass supernatants,assayed
R11600 T13064 T13061 appos ng/ml,IL-1β
R11601 T13065 T13061 punct ),IL-1β
R11602 T13066 T13052 punct .,Insert
R11746 T13246 T13247 compound Airway,cells
R11747 T13247 T13251 nsubj cells,upregulate
R11748 T13248 T13251 aux do,upregulate
R11749 T13249 T13251 neg not,upregulate
R11750 T13250 T13251 advmod strongly,upregulate
R11751 T13251 T13251 ROOT upregulate,upregulate
R11752 T13252 T13253 amod caspase-1,activity
R11753 T13253 T13251 dobj activity,upregulate
R11754 T13254 T13251 prep in,upregulate
R11755 T13255 T13254 pobj response,in
R11756 T13256 T13255 prep to,response
R11757 T13257 T13258 amod inflammasome,stimuli
R11758 T13258 T13256 pobj stimuli,to
R11759 T13259 T13251 punct .,upregulate
R11760 T13260 T13263 nummod S9,cells
R11761 T13261 T13260 cc and,S9
R11762 T13262 T13260 conj IB3-1,S9
R11763 T13263 T13265 nsubjpass cells,examined
R11764 T13264 T13265 auxpass were,examined
R11765 T13265 T13265 ROOT examined,examined
R11766 T13266 T13265 prep for,examined
R11767 T13267 T13268 amod caspase-1,activation
R11768 T13268 T13266 pobj activation,for
R11769 T13269 T13265 prep following,examined
R11836 T13336 T13338 nummod ×,cells/well
R11837 T13337 T13338 nummod 104,cells/well
R11838 T13338 T13333 appos cells/well,cells
R11839 T13339 T13333 punct ),cells
R11840 T13340 T13340 ROOT were,were
R11841 T13341 T13340 acomp pre-treated,were
R11842 T13342 T13341 prep for,pre-treated
R11843 T13343 T13344 nummod 1,hour
R11844 T13344 T13342 pobj hour,for
R11845 T13345 T13344 prep with,hour
R11846 T13346 T13347 amod increasing,concentrations
R11847 T13347 T13345 pobj concentrations,with
R11848 T13348 T13347 prep of,concentrations
R11849 T13349 T13348 pobj z-YVAD-fmk,of
R11850 T13350 T13349 punct (,z-YVAD-fmk
R11851 T13351 T13349 npadvmod 10,z-YVAD-fmk
R11852 T13352 T13353 nummod 30,µM
R11853 T13353 T13349 appos µM,z-YVAD-fmk
R11854 T13354 T13349 punct ),z-YVAD-fmk
R11855 T13355 T13340 advmod prior,were
R11856 T13356 T13355 prep to,prior
R11857 T13357 T13356 pobj stimulation,to
R11858 T13358 T13355 prep with,prior
R11859 T13359 T13360 compound P.,aeruginosa
R11860 T13360 T13358 pobj aeruginosa,with
R11861 T13361 T13362 punct (,MOI
R11862 T13362 T13360 appos MOI,aeruginosa
R11863 T13363 T13364 punct =,50
R11864 T13364 T13362 nummod 50,MOI
R11865 T13365 T13362 punct ),MOI
R11866 T13366 T13340 punct .,were
R11867 T13367 T13369 compound Cell,supernatants
R11868 T13368 T13369 compound culture,supernatants
R11869 T13369 T13371 nsubjpass supernatants,collected
R11870 T13370 T13371 auxpass were,collected
R11871 T13371 T13371 ROOT collected,collected
R11872 T13372 T13371 prep after,collected
R11873 T13373 T13374 nummod 6,hours
R11874 T13374 T13372 pobj hours,after
R11875 T13375 T13371 cc and,collected
R11876 T13376 T13371 conj assayed,collected
R11877 T13377 T13376 prep for,assayed
R11878 T13378 T13377 pobj IL-6,for
R11879 T13379 T13376 agent by,assayed
R11880 T13380 T13379 pobj ELISA,by
R11881 T13381 T13384 punct (,3
R11882 T13382 T13384 advmod n,3
R11883 T13383 T13384 punct =,3
R11884 T13384 T13384 ROOT 3,3
R11885 T13385 T13384 punct ),3
R11886 T13386 T13371 punct .,collected
R11986 T13509 T13515 nsubj PBMCs,show
R11987 T13510 T13509 prep from,PBMCs
R11988 T13511 T13512 compound CF,patients
R11989 T13512 T13510 pobj patients,from
R11990 T13513 T13512 cc and,patients
R11991 T13514 T13512 conj controls,patients
R11992 T13515 T13515 ROOT show,show
R11993 T13516 T13517 amod similar,increases
R11994 T13517 T13515 dobj increases,show
R11995 T13518 T13517 prep in,increases
R11996 T13519 T13520 amod caspase-1,activity
R11997 T13520 T13518 pobj activity,in
R11998 T13521 T13517 prep upon,increases
R11999 T13522 T13523 amod inflammasome,activation
R12000 T13523 T13521 pobj activation,upon
R12001 T13524 T13515 punct .,show
R12002 T13525 T13543 nsubjpass PBMCs,primed
R12003 T13526 T13525 prep from,PBMCs
R12004 T13527 T13528 compound CF,patients
R12005 T13528 T13526 pobj patients,from
R12006 T13529 T13528 punct (,patients
R12007 T13530 T13543 nsubjpass n,primed
R12008 T13531 T13532 punct =,6
R12009 T13532 T13530 prep 6,n
R12010 T13533 T13530 punct ),n
R12011 T13534 T13530 cc and,n
R12012 T13535 T13536 amod healthy,controls
R12013 T13536 T13530 conj controls,n
R12014 T13537 T13540 punct (,6
R12015 T13538 T13540 advmod n,6
R12016 T13539 T13540 punct =,6
R12017 T13540 T13536 appos 6,controls
R12018 T13541 T13536 punct ),controls
R12019 T13542 T13543 auxpass were,primed
R12020 T13543 T13543 ROOT primed,primed
R12021 T13544 T13543 prep with,primed
R12022 T13545 T13544 pobj LPS,with
R12023 T13546 T13545 punct (,LPS
R12024 T13547 T13548 nummod 10,ng/ml
R12025 T13548 T13545 appos ng/ml,LPS
R12026 T13549 T13545 punct ),LPS
R12027 T13550 T13543 prep for,primed
R12028 T13551 T13552 nummod 5,hours
R12029 T13552 T13550 pobj hours,for
R12030 T13553 T13543 advmod prior,primed
R12031 T13554 T13553 prep to,prior
R12032 T13555 T13554 pobj stimulation,to
R12033 T13556 T13543 prep with,primed
R12034 T13557 T13556 pobj ATP,with
R12035 T13558 T13557 punct (,ATP
R12036 T13559 T13560 nummod 5,mM
R12037 T13560 T13557 appos mM,ATP
R12038 T13561 T13557 punct ),ATP
R12039 T13562 T13543 prep for,primed
R12040 T13563 T13564 nummod 1,hour
R12041 T13564 T13562 pobj hour,for
R12042 T13565 T13564 cc or,hour
R12043 T13566 T13564 conj Poly,hour
R12044 T13567 T13566 punct (,Poly
R12045 T13568 T13566 nmod dA,Poly
R12046 T13569 T13566 punct :,Poly
R12047 T13570 T13566 appos dT,Poly
R12048 T13571 T13566 punct ),Poly
R12081 T13604 T13603 prep in,shown
R12082 T13605 T13606 punct (,A
R12083 T13606 T13604 pobj A,in
R12084 T13607 T13606 punct ),A
R12085 T13608 T13603 prep with,shown
R12086 T13609 T13611 det the,values
R12087 T13610 T13611 amod averaged,values
R12088 T13611 T13608 pobj values,with
R12089 T13612 T13611 acl shown,values
R12075 T13598 T13601 det the,cells
R12076 T13599 T13601 nmod %,cells
R12077 T13600 T13601 amod caspase-1-active,cells
R12078 T13601 T13597 pobj cells,of
R12079 T13602 T13603 auxpass is,shown
R12080 T13603 T13603 ROOT shown,shown
R12240 T13785 T13785 ROOT primed,primed
R12241 T13786 T13785 prep with,primed
R12242 T13787 T13786 pobj LPS,with
R12243 T13788 T13787 punct (,LPS
R12244 T13789 T13790 nummod 10,ng/ml
R12245 T13790 T13787 appos ng/ml,LPS
R12246 T13791 T13790 punct ),ng/ml
R12247 T13792 T13787 conj overnight,LPS
R12248 T13793 T13792 cc and,overnight
R12249 T13794 T13792 conj stimulated,overnight
R12250 T13795 T13794 prep with,stimulated
R12251 T13796 T13798 compound P.,PAO1
R12252 T13797 T13798 compound aeruginosa,PAO1
R12253 T13798 T13795 pobj PAO1,with
R12254 T13799 T13808 punct (,lacking
R12255 T13800 T13808 nsubj MOI,lacking
R12256 T13801 T13802 punct =,1
R12257 T13802 T13800 nummod 1,MOI
R12258 T13803 T13802 punct ),1
R12259 T13804 T13800 punct ",",MOI
R12293 T13838 T13808 punct .,lacking
R12294 T13839 T13840 compound P.,aeruginosa
R12295 T13840 T13844 nsubjpass aeruginosa,used
R12296 T13841 T13840 acl lacking,aeruginosa
R12297 T13842 T13841 dobj exsA,lacking
R12298 T13843 T13844 auxpass was,used
R12299 T13844 T13844 ROOT used,used
R12300 T13845 T13844 prep as,used
R12301 T13846 T13850 det a,control
R12302 T13847 T13850 compound type,control
R12303 T13848 T13850 compound III,control
R12304 T13849 T13850 compound secretion,control
R12305 T13850 T13845 pobj control,as
R12306 T13851 T13850 prep in,control
R12307 T13852 T13851 pobj comparison,in
R12308 T13853 T13852 prep with,comparison
R12309 T13854 T13856 amod wild-type,aeruginosa
R12310 T13855 T13856 compound P.,aeruginosa
R12311 T13856 T13853 pobj aeruginosa,with
R12312 T13857 T13844 punct .,used
R12313 T13858 T13860 nsubjpass Supernatants,assayed
R12314 T13859 T13860 auxpass were,assayed
R12315 T13860 T13860 ROOT assayed,assayed
R12316 T13861 T13860 prep for,assayed
R12317 T13862 T13863 punct (,A
R12318 T13863 T13861 pobj A,for
R12319 T13864 T13863 punct ),A
R12320 T13865 T13860 advcl IL-1β,assayed
R12321 T13866 T13865 cc and,IL-1β
R12322 T13867 T13868 punct (,B
R12323 T13868 T13865 conj B,IL-1β
R12324 T13869 T13868 punct ),B
R12325 T13870 T13865 conj IL-8,IL-1β
R12326 T13871 T13860 punct .,assayed
R12651 T14237 T14236 punct ),B
R12652 T14238 T14233 appos IL-8,activity
R12653 T14239 T14238 punct ",",IL-8
R12654 T14240 T14238 cc and,IL-8
R12655 T14241 T14242 punct (,C
R12656 T14242 T14245 nmod C,secretion
R12657 T14243 T14242 punct ),C
R12658 T14244 T14245 amod IL-1β,secretion
R12659 T14245 T14238 conj secretion,IL-8
R12660 T14246 T14247 punct (,n
R12661 T14247 T14238 conj n,IL-8
R12662 T14248 T14249 punct =,3
R12663 T14249 T14247 prep 3,n
R12664 T14250 T14251 nummod 6,experiments
R12665 T14251 T14249 appos experiments,3
R12701 T14287 T14286 prep at,assayed
R12666 T14252 T14249 punct ),3
R12667 T14253 T14225 punct .,assayed
R12668 T14254 T14264 advcl Using,treated
R12669 T14255 T14258 det the,method
R12670 T14256 T14258 amod same,method
R12671 T14257 T14258 compound stimulation,method
R12672 T14258 T14254 dobj method,Using
R12673 T14259 T14264 punct ",",treated
R12674 T14260 T14262 nummod THP-1,cells
R12675 T14261 T14262 compound reporter,cells
R12676 T14262 T14264 nsubjpass cells,treated
R12677 T14263 T14264 auxpass were,treated
R12678 T14264 T14264 ROOT treated,treated
R12679 T14265 T14264 prep with,treated
R12680 T14266 T14265 pobj Bay11-7082,with
R12681 T14267 T14269 punct (,µM
R12682 T14268 T14269 nummod 20,µM
R12683 T14269 T14266 appos µM,Bay11-7082
R12684 T14270 T14269 punct ),µM
R12685 T14271 T14264 prep for,treated
R12686 T14272 T14273 nummod 1,hour
R12687 T14273 T14271 pobj hour,for
R12688 T14274 T14264 advmod prior,treated
R12689 T14275 T14274 prep to,prior
R12690 T14276 T14275 pobj priming,to
R12691 T14277 T14276 prep with,priming
R12692 T14278 T14279 compound heat-killed,PAO1
R12693 T14279 T14277 pobj PAO1,with
R12694 T14280 T14264 cc or,treated
R12695 T14281 T14264 conj live,treated
R12090 T13613 T13612 prep in,shown
R12091 T13614 T13615 punct (,B
R12092 T13615 T13613 pobj B,in
R12093 T13616 T13603 punct ),shown
R12094 T13617 T13603 punct .,shown
R12214 T13759 T13759 ROOT produce,produce
R12208 T13753 T13759 nsubj PBMCs,produce
R12209 T13754 T13753 prep from,PBMCs
R12210 T13755 T13756 compound CF,patients
R12211 T13756 T13754 pobj patients,from
R12212 T13757 T13759 aux do,produce
R12213 T13758 T13759 neg not,produce
R12215 T13760 T13761 amod increased,amounts
R12216 T13761 T13759 dobj amounts,produce
R12217 T13762 T13761 prep of,amounts
R12218 T13763 T13762 pobj IL-1β,of
R12219 T13764 T13759 punct .,produce
R12220 T13765 T13770 nsubj PBMCs,n
R12221 T13766 T13765 prep from,PBMCs
R12222 T13767 T13768 compound CF,patients
R12223 T13768 T13766 pobj patients,from
R12224 T13769 T13768 punct (,patients
R12225 T13770 T13770 ROOT n,n
R12226 T13771 T13772 punct =,17
R12227 T13772 T13770 nummod 17,n
R12228 T13773 T13772 appos 20,17
R12229 T13774 T13772 punct ),17
R12230 T13775 T13770 cc and,n
R12231 T13776 T13777 amod healthy,controls
R12232 T13777 T13770 conj controls,n
R12233 T13778 T13777 punct (,controls
R12234 T13779 T13785 nsubjpass n,primed
R12235 T13780 T13781 punct =,15
R12236 T13781 T13779 prep 15,n
R12237 T13782 T13781 appos 19,15
R12238 T13783 T13781 punct ),15
R12239 T13784 T13785 auxpass were,primed
R12260 T13805 T13807 compound P.,PAO1
R12261 T13806 T13807 compound aeruginosa,PAO1
R12262 T13807 T13808 nsubj PAO1,lacking
R12263 T13808 T13808 ROOT lacking,lacking
R12264 T13809 T13808 dobj exsA,lacking
R12265 T13810 T13819 punct (,mM
R12266 T13811 T13819 nmod MOI,mM
R12267 T13812 T13813 punct =,1
R12268 T13813 T13811 nummod 1,MOI
R12269 T13814 T13813 punct ),1
R12270 T13815 T13811 punct ",",MOI
R12271 T13816 T13811 conj ATP,MOI
R12272 T13817 T13818 punct (,5
R12273 T13818 T13819 nummod 5,mM
R12274 T13819 T13809 appos mM,exsA
R12275 T13820 T13819 punct ),mM
R12276 T13821 T13809 punct ",",exsA
R12277 T13822 T13809 cc or,exsA
R12278 T13823 T13809 conj Poly,exsA
R12279 T13824 T13823 punct (,Poly
R12280 T13825 T13833 nmod dA,ml
R12281 T13826 T13825 punct :,dA
R12282 T13827 T13825 appos dT,dA
R12283 T13828 T13825 punct ),dA
R12284 T13829 T13833 punct (,ml
R12285 T13830 T13831 nummod 1,µg
R12286 T13831 T13833 nmod µg,ml
R12287 T13832 T13833 compound /,ml
R12288 T13833 T13823 appos ml,Poly
R12289 T13834 T13823 punct ),Poly
R12290 T13835 T13808 prep for,lacking
R12291 T13836 T13837 nummod 24,hours
R12292 T13837 T13835 pobj hours,for
R12592 T14178 T14179 amod NF-κB,activation
R12593 T14179 T14180 nsubj activation,potentiates
R12594 T14180 T14180 ROOT potentiates,potentiates
R12595 T14181 T14182 det the,degree
R12596 T14182 T14180 dobj degree,potentiates
R12597 T14183 T14182 prep of,degree
R12598 T14184 T14185 amod IL-1β,production
R12599 T14185 T14183 pobj production,of
R12600 T14186 T14185 cc and,production
R12601 T14187 T14185 conj secretion,production
R12602 T14188 T14185 prep upon,production
R12603 T14189 T14190 amod inflammasome,activation
R12604 T14190 T14188 pobj activation,upon
R12605 T14191 T14180 punct .,potentiates
R12606 T14192 T14194 nummod THP-1,cells
R12607 T14193 T14194 compound reporter,cells
R12608 T14194 T14196 nsubjpass cells,primed
R12609 T14195 T14196 auxpass were,primed
R12610 T14196 T14196 ROOT primed,primed
R12611 T14197 T14196 npadvmod overnight,primed
R12612 T14198 T14197 prep with,overnight
R12613 T14199 T14201 amod heat-killed,aeruginosa
R12614 T14200 T14201 compound P.,aeruginosa
R12615 T14201 T14198 pobj aeruginosa,with
R12616 T14202 T14196 cc and,primed
R12617 T14203 T14196 conj stimulated,primed
R12618 T14204 T14206 det the,day
R12619 T14205 T14206 amod next,day
R12620 T14206 T14203 dobj day,stimulated
R12621 T14207 T14203 prep with,stimulated
R12622 T14208 T14210 amod live,aeruginosa
R12623 T14209 T14210 compound P.,aeruginosa
R12624 T14210 T14207 pobj aeruginosa,with
R12625 T14211 T14210 cc or,aeruginosa
R12626 T14212 T14215 amod additional,aeruginosa
R12627 T14213 T14215 amod heat-killed,aeruginosa
R12628 T14214 T14215 compound P.,aeruginosa
R12629 T14215 T14210 conj aeruginosa,aeruginosa
R12630 T14216 T14215 prep for,aeruginosa
R12631 T14217 T14218 det the,times
R12632 T14218 T14216 pobj times,for
R12633 T14219 T14203 conj indicated,stimulated
R12634 T14220 T14196 punct .,primed
R12635 T14221 T14223 compound Cell,supernatants
R12636 T14222 T14223 compound culture,supernatants
R12637 T14223 T14225 nsubjpass supernatants,assayed
R12638 T14224 T14225 auxpass were,assayed
R12639 T14225 T14225 ROOT assayed,assayed
R12640 T14226 T14225 prep for,assayed
R12641 T14227 T14228 punct (,A
R12642 T14228 T14233 nmod A,activity
R12643 T14229 T14228 punct ),A
R12644 T14230 T14233 nmod NF-κB,activity
R12645 T14231 T14233 nmod /,activity
R12646 T14232 T14233 compound AP-1,activity
R12647 T14233 T14226 pobj activity,for
R12648 T14234 T14233 punct ",",activity
R12649 T14235 T14238 punct (,IL-8
R12650 T14236 T14238 nmod B,IL-8
R12721 T14307 T14306 punct ),F
R12722 T14308 T14309 compound IL-1β,secretion
R12723 T14309 T14297 appos secretion,activity
R12724 T14310 T14313 punct (,3
R12725 T14311 T14313 advmod n,3
R12726 T14312 T14313 punct =,3
R12727 T14313 T14309 nummod 3,secretion
R12728 T14314 T14336 meta 5,treated
R12729 T14315 T14314 punct ),5
R12730 T14316 T14314 punct .,5
R12731 T14317 T14336 nsubjpass PBMCs,treated
R12732 T14318 T14317 prep from,PBMCs
R12733 T14319 T14320 compound CF,patients
R12734 T14320 T14318 pobj patients,from
R12735 T14321 T14320 punct (,patients
R12736 T14322 T14336 nsubjpass n,treated
R12737 T14323 T14324 punct =,11
R12738 T14324 T14322 prep 11,n
R12739 T14325 T14324 appos 15,11
R12740 T14326 T14324 punct ),11
R12741 T14327 T14322 cc and,n
R12742 T14328 T14322 conj controls,n
R12743 T14329 T14332 punct (,10
R12744 T14330 T14332 advmod n,10
R12745 T14331 T14332 punct =,10
R12746 T14332 T14322 appos 10,n
R12747 T14333 T14332 appos 13,10
R12748 T14334 T14332 punct ),10
R12749 T14335 T14336 auxpass were,treated
R12696 T14282 T14281 npadvmod PAO1,live
R12697 T14283 T14264 punct .,treated
R12698 T14284 T14286 nsubjpass Supernatants,assayed
R12699 T14285 T14286 auxpass were,assayed
R12700 T14286 T14286 ROOT assayed,assayed
R12702 T14288 T14289 nummod 24,hours
R12703 T14289 T14287 pobj hours,at
R12704 T14290 T14286 prep for,assayed
R12705 T14291 T14292 punct (,D
R12706 T14292 T14290 pobj D,for
R12707 T14293 T14292 punct ),D
R12708 T14294 T14296 compound NF-κB,AP-1
R12709 T14295 T14296 compound /,AP-1
R12710 T14296 T14297 compound AP-1,activity
R12711 T14297 T14297 ROOT activity,activity
R12712 T14298 T14297 punct ",",activity
R12713 T14299 T14302 punct (,IL-8
R12714 T14300 T14302 nmod E,IL-8
R12715 T14301 T14302 punct ),IL-8
R12716 T14302 T14309 nmod IL-8,secretion
R12717 T14303 T14302 punct ",",IL-8
R12718 T14304 T14302 cc and,IL-8
R12719 T14305 T14306 punct (,F
R12720 T14306 T14302 conj F,IL-8
R12796 T14382 T14380 pobj schedule,to
R12797 T14383 T14382 prep in,schedule
R12798 T14384 T14383 pobj Figure,in
R12799 T14385 T14384 nummod 1,Figure
R12800 T14386 T14286 punct .,assayed
R12801 T14387 T14390 punct (,IL-1β
R12802 T14388 T14390 nmod G,IL-1β
R12803 T14389 T14390 punct ),IL-1β
R12804 T14390 T14396 nmod IL-1β,levels
R12805 T14391 T14390 cc and,IL-1β
R12806 T14392 T14393 punct (,H
R12807 T14393 T14390 conj H,IL-1β
R12808 T14394 T14393 punct ),H
R12809 T14395 T14396 compound IL-8,levels
R12810 T14396 T14398 nsubjpass levels,measured
R12811 T14397 T14398 auxpass were,measured
R12812 T14398 T14398 ROOT measured,measured
R12813 T14399 T14398 prep at,measured
R12814 T14400 T14401 nummod 24,hours
R12815 T14401 T14399 pobj hours,at
R12816 T14402 T14398 punct .,measured
R12817 T14403 T14404 compound Statistical,analysis
R12818 T14404 T14406 nsubjpass analysis,performed
R12819 T14405 T14406 auxpass was,performed
R12820 T14406 T14406 ROOT performed,performed
R12821 T14407 T14406 advcl using,performed
R12822 T14408 T14409 nummod two,way
R12823 T14409 T14410 advmod way,ANOVA
R12824 T14410 T14407 dobj ANOVA,using
R12825 T14411 T14410 prep with,ANOVA
R12826 T14412 T14413 compound Bonferroni,correction
R12827 T14413 T14411 pobj correction,with
R12828 T14414 T14413 prep for,correction
R13010 T14637 T14636 prep of,graph
R13011 T14638 T14642 det the,values
R13012 T14639 T14642 amod averaged,values
R13013 T14640 T14642 compound fluorescence,values
R13014 T14641 T14642 compound intensity,values
R13015 T14642 T14637 pobj values,of
R13016 T14643 T14642 prep over,values
R13017 T14644 T14645 nummod 3,experiments
R13018 T14645 T14643 pobj experiments,over
R13019 T14646 T14633 punct .,shown
R13167 T14813 T14819 nsubj Disruption,increase
R13168 T14814 T14813 prep of,Disruption
R13169 T14815 T14816 amod CFTR,activity
R13170 T14816 T14814 pobj activity,of
R13171 T14817 T14819 aux does,increase
R13172 T14818 T14819 neg not,increase
R13173 T14819 T14819 ROOT increase,increase
R13174 T14820 T14821 amod IL-1β,production
R13175 T14821 T14819 dobj production,increase
R13176 T14822 T14821 prep in,production
R13177 T14823 T14822 pobj PBMCs,in
R13178 T14824 T14823 cc or,PBMCs
R13179 T14825 T14823 conj macrophages,PBMCs
R13180 T14826 T14819 punct .,increase
R13181 T14827 T14844 nsubjpass PBMCs,treated
R13182 T14828 T14827 prep from,PBMCs
R13183 T14829 T14830 compound CF,patients
R13184 T14830 T14828 pobj patients,from
R13185 T14831 T14830 punct (,patients
R13186 T14832 T14834 nmod n,15
R13187 T14833 T14834 punct =,15
R13188 T14834 T14827 appos 15,PBMCs
R13189 T14835 T14827 punct ),PBMCs
R13190 T14836 T14827 cc and,PBMCs
R13191 T14837 T14827 conj controls,PBMCs
R13192 T14838 T14837 punct (,controls
R13193 T14839 T14841 advmod n,13
R13194 T14840 T14841 punct =,13
R13195 T14841 T14837 appos 13,controls
R13196 T14842 T14837 punct ),controls
R13197 T14843 T14844 auxpass were,treated
R13198 T14844 T14844 ROOT treated,treated
R13199 T14845 T14844 prep with,treated
R13200 T14846 T14845 pobj CFTRinh172,with
R13201 T14847 T14849 punct (,µM
R13202 T14848 T14849 nummod 10,µM
R13203 T14849 T14846 appos µM,CFTRinh172
R13204 T14850 T14844 punct ),treated
R13205 T14851 T14844 prep for,treated
R13206 T14852 T14853 nummod 18,hours
R13207 T14853 T14851 pobj hours,for
R13208 T14854 T14844 advmod prior,treated
R13209 T14855 T14854 prep to,prior
R13210 T14856 T14855 pobj stimulation,to
R13211 T14857 T14844 prep with,treated
R12750 T14336 T14286 advcl treated,assayed
R12751 T14337 T14336 prep with,treated
R12752 T14338 T14337 pobj z-YVAD-fmk,with
R12753 T14339 T14341 punct (,µM
R12754 T14340 T14341 nummod 20,µM
R12755 T14341 T14338 appos µM,z-YVAD-fmk
R12756 T14342 T14341 punct ),µM
R12757 T14343 T14338 cc or,z-YVAD-fmk
R12758 T14344 T14338 conj Bay11-7082,z-YVAD-fmk
R12759 T14345 T14347 punct (,µM
R12760 T14346 T14347 nummod 10,µM
R12761 T14347 T14344 appos µM,Bay11-7082
R12762 T14348 T14347 punct ),µM
R12763 T14349 T14336 cc and,treated
R12764 T14350 T14336 conj stimulated,treated
R12765 T14351 T14350 prep with,stimulated
R12766 T14352 T14353 amod live,PAO1
R12767 T14353 T14351 pobj PAO1,with
R12768 T14354 T14377 punct (,ml
R12769 T14355 T14377 nmod MOI,ml
R12770 T14356 T14357 punct =,1
R12771 T14357 T14355 nummod 1,MOI
R12772 T14358 T14357 punct ),1
R12773 T14359 T14355 punct ",",MOI
R12774 T14360 T14355 conj ATP,MOI
R12775 T14361 T14362 punct (,5
R12776 T14362 T14363 nummod 5,mM
R12777 T14363 T14355 conj mM,MOI
R12778 T14364 T14363 punct ),mM
R12779 T14365 T14355 punct ",",MOI
R12780 T14366 T14355 cc or,MOI
R12781 T14367 T14355 conj Poly,MOI
R12782 T14368 T14367 punct (,Poly
R12783 T14369 T14377 nmod dA,ml
R12784 T14370 T14369 punct :,dA
R12785 T14371 T14369 appos dT,dA
R12786 T14372 T14369 punct ),dA
R12787 T14373 T14377 punct (,ml
R12788 T14374 T14375 nummod 1,µg
R12789 T14375 T14377 nmod µg,ml
R12790 T14376 T14377 compound /,ml
R12791 T14377 T14353 appos ml,PAO1
R12792 T14378 T14377 punct ),ml
R12793 T14379 T14286 prep according,assayed
R12794 T14380 T14379 prep to,according
R12795 T14381 T14382 det the,schedule
R12829 T14415 T14416 amod multiple,comparisons
R12830 T14416 T14414 pobj comparisons,for
R12831 T14417 T14406 punct .,performed
R12832 T14418 T14424 punct *,signify
R12833 T14419 T14424 punct ",",signify
R12834 T14420 T14424 nsubj **,signify
R12835 T14421 T14420 punct ",",**
R12836 T14422 T14420 cc and,**
R12837 T14423 T14424 advmod ***,signify
R12838 T14424 T14424 ROOT signify,signify
R12839 T14425 T14424 dobj P,signify
R12840 T14426 T14425 nummod <,P
R12841 T14427 T14425 nummod 0.05,P
R12842 T14428 T14425 punct ",",P
R12843 T14429 T14425 appos 0.01,P
R12844 T14430 T14425 punct ",",P
R12845 T14431 T14425 cc and,P
R12846 T14432 T14425 conj 0.001,P
R12847 T14433 T14424 punct .,signify
R12946 T14573 T14574 amod Bay11-7082,inhibits
R12947 T14574 T14574 ROOT inhibits,inhibits
R12948 T14575 T14576 compound pro-IL-1β,production
R12949 T14576 T14574 dobj production,inhibits
R12950 T14577 T14574 prep in,inhibits
R12951 T14578 T14577 pobj response,in
R12952 T14579 T14578 prep to,response
R12953 T14580 T14581 compound P.,aeruginosa
R12954 T14581 T14579 pobj aeruginosa,to
R12955 T14582 T14574 punct .,inhibits
R12956 T14583 T14585 amod PMA-differentiated,cells
R12957 T14584 T14585 compound THP-1,cells
R12958 T14585 T14587 nsubjpass cells,treated
R12959 T14586 T14587 auxpass were,treated
R12960 T14587 T14587 ROOT treated,treated
R12961 T14588 T14587 prep with,treated
R12962 T14589 T14591 nummod 10,CFTRinh172
R12963 T14590 T14591 compound µM,CFTRinh172
R12964 T14591 T14588 pobj CFTRinh172,with
R12965 T14592 T14591 cc or,CFTRinh172
R12966 T14593 T14595 nummod 20,Bay11-7082
R12967 T14594 T14595 compound µM,Bay11-7082
R12968 T14595 T14591 conj Bay11-7082,CFTRinh172
R12969 T14596 T14587 cc and,treated
R12970 T14597 T14587 conj harvested,treated
R12971 T14598 T14597 prep after,harvested
R12972 T14599 T14600 punct (,A
R12973 T14600 T14598 pobj A,after
R12974 T14601 T14600 punct ),A
R12975 T14602 T14603 nummod 4,hours
R12976 T14603 T14605 npadvmod hours,n
R12977 T14604 T14603 punct (,hours
R12978 T14605 T14597 advmod n,harvested
R12979 T14606 T14607 punct =,3
R12980 T14607 T14605 pobj 3,n
R12981 T14608 T14605 punct ),n
R12982 T14609 T14597 cc or,harvested
R12983 T14610 T14611 punct (,B
R12984 T14611 T14597 conj B,harvested
R12985 T14612 T14611 punct ),B
R12986 T14613 T14611 nummod 0.5,B
R12987 T14614 T14611 punct ",",B
R12988 T14615 T14611 nummod 1,B
R12989 T14616 T14611 punct ",",B
R12990 T14617 T14611 cc and,B
R12991 T14618 T14619 nummod 1.5,hours
R12992 T14619 T14621 npadvmod hours,n
R12993 T14620 T14619 punct (,hours
R12994 T14621 T14611 conj n,B
R12995 T14622 T14623 punct =,3
R12996 T14623 T14625 meta 3,stimulation
R12997 T14624 T14623 punct ),3
R12998 T14625 T14611 appos stimulation,B
R12999 T14626 T14625 prep with,stimulation
R13000 T14627 T14626 pobj PAO1,with
R13001 T14628 T14587 punct .,treated
R13002 T14629 T14631 nummod One,blot
R13003 T14630 T14631 compound representative,blot
R13004 T14631 T14633 nsubjpass blot,shown
R13005 T14632 T14633 auxpass is,shown
R13006 T14633 T14633 ROOT shown,shown
R13007 T14634 T14633 prep with,shown
R13008 T14635 T14636 det a,graph
R13009 T14636 T14634 pobj graph,with
R13235 T14881 T14877 punct .,measured
R13236 T14882 T14883 punct (,C
R13237 T14883 T14883 ROOT C,C
R13238 T14884 T14883 punct ),C
R13239 T14885 T14894 nsubjpass Monocytes,differentiated
R13240 T14886 T14885 prep from,Monocytes
R13241 T14887 T14886 pobj controls,from
R13242 T14888 T14887 punct (,controls
R13243 T14889 T14887 appos n,controls
R13244 T14890 T14887 punct =,controls
R13245 T14891 T14894 nsubjpass 3,differentiated
R13246 T14892 T14891 punct ),3
R13247 T14893 T14894 auxpass were,differentiated
R13248 T14894 T14894 ROOT differentiated,differentiated
R13249 T14895 T14894 prep into,differentiated
R13250 T14896 T14895 pobj macrophages,into
R13251 T14897 T14894 punct .,differentiated
R13252 T14898 T14900 nsubjpass Macrophages,treated
R13253 T14899 T14900 auxpass were,treated
R13254 T14900 T14900 ROOT treated,treated
R13255 T14901 T14900 prep with,treated
R13256 T14902 T14901 pobj CFTRinh172,with
R13257 T14903 T14904 punct ",",stimulated
R13258 T14904 T14900 advcl stimulated,treated
R13259 T14905 T14904 prep as,stimulated
R13260 T14906 T14905 prep per,as
R13261 T14907 T14906 pobj monocytes,per
R13262 T14908 T14904 punct ",",stimulated
R13263 T14909 T14904 cc and,stimulated
R13212 T14858 T14859 amod live,PAO1
R13213 T14859 T14857 pobj PAO1,with
R13214 T14860 T14861 punct (,MOI
R13215 T14861 T14859 appos MOI,PAO1
R13216 T14862 T14863 punct =,1
R13217 T14863 T14861 nummod 1,MOI
R13218 T14864 T14863 punct ),1
R13219 T14865 T14844 punct .,treated
R13220 T14866 T14869 punct (,IL-1β
R13221 T14867 T14869 nmod A,IL-1β
R13222 T14868 T14867 punct ),A
R13223 T14869 T14877 nsubjpass IL-1β,measured
R13224 T14870 T14869 cc and,IL-1β
R13225 T14871 T14872 punct (,B
R13226 T14872 T14875 nmod B,production
R13227 T14873 T14872 punct ),B
R13228 T14874 T14875 compound IL-8,production
R13229 T14875 T14869 conj production,IL-1β
R13230 T14876 T14877 auxpass was,measured
R13231 T14877 T14877 ROOT measured,measured
R13232 T14878 T14877 prep at,measured
R13233 T14879 T14880 nummod 24,hours
R13234 T14880 T14878 pobj hours,at
R13310 T14956 T14954 pobj hours,at
R13311 T14957 T14956 punct (,hours
R13312 T14958 T14956 parataxis n,hours
R13313 T14959 T14960 punct =,4
R13314 T14960 T14956 nummod 4,hours
R13315 T14961 T14950 punct ),NF-κB
R13316 T14962 T14922 punct .,treated
R13338 T15016 T15017 compound Inflammasome,Activators
R13264 T14910 T14904 conj measured,stimulated
R13265 T14911 T14910 prep for,measured
R13266 T14912 T14913 compound IL-1β,production
R13267 T14913 T14911 pobj production,for
R13268 T14914 T14910 prep at,measured
R13269 T14915 T14916 nummod 24,hours
R13270 T14916 T14914 pobj hours,at
R13271 T14917 T14900 punct .,treated
R13272 T14918 T14920 nummod THP-1,cells
R13273 T14919 T14920 compound reporter,cells
R13274 T14920 T14922 nsubjpass cells,treated
R13275 T14921 T14922 auxpass were,treated
R13276 T14922 T14922 ROOT treated,treated
R13277 T14923 T14922 prep with,treated
R13278 T14924 T14923 pobj CFTRinh172,with
R13279 T14925 T14926 nummod 24,hours
R13280 T14926 T14927 npadvmod hours,prior
R13281 T14927 T14922 advmod prior,treated
R13282 T14928 T14927 prep to,prior
R13283 T14929 T14928 pobj stimulation,to
R13284 T14930 T14922 prep with,treated
R13285 T14931 T14930 pobj PAO1,with
R13286 T14932 T14922 cc and,treated
R13287 T14933 T14922 conj measured,treated
R13288 T14934 T14933 prep for,measured
R13289 T14935 T14936 punct (,D
R13290 T14936 T14934 pobj D,for
R13291 T14937 T14933 punct ),measured
R13292 T14938 T14939 amod IL-1β,secretion
R13293 T14939 T14939 ROOT secretion,secretion
R13294 T14940 T14939 punct ",",secretion
R13295 T14941 T14944 punct (,IL-8
R13296 T14942 T14944 nmod E,IL-8
R13297 T14943 T14944 punct ),IL-8
R13298 T14944 T14939 appos IL-8,secretion
R13299 T14945 T14944 punct ",",IL-8
R13300 T14946 T14944 cc and,IL-8
R13301 T14947 T14948 punct (,F
R13302 T14948 T14950 nmod F,NF-κB
R13303 T14949 T14950 punct ),NF-κB
R13304 T14950 T14944 conj NF-κB,IL-8
R13305 T14951 T14953 nmod /,activity
R13306 T14952 T14953 compound AP-1,activity
R13307 T14953 T14950 dobj activity,NF-κB
R13308 T14954 T14950 prep at,NF-κB
R13309 T14955 T14956 nummod 24,hours
R13339 T15017 T15017 ROOT Activators,Activators
R13340 T15018 T15017 punct .,Activators

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T4674 13554-13559 Anaphor denotes which
T4675 13519-13528 Antecedent denotes inhibitor
T7384 17922-17925 Anaphor denotes its
T7385 17830-17835 Antecedent denotes IL-1β
R4083 T4674 T4675 boundBy which,inhibitor
R6519 T7384 T7385 boundBy its,IL-1β

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T89 0-21 JJ denotes Inflammasome-Mediated
T90 22-27 NN denotes IL-1β
T91 28-38 NN denotes Production
T92 39-41 IN denotes in
T93 42-48 NN denotes Humans
T94 49-53 IN denotes with
T95 54-60 JJ denotes Cystic
T96 61-69 NN denotes Fibrosis
T97 117-127 NN denotes Background
T98 128-140 NN denotes Inflammation
T99 141-144 CC denotes and
T100 145-154 NN denotes infection
T101 155-158 VB denotes are
T102 159-164 JJ denotes major
T103 165-177 NN denotes determinants
T104 178-180 IN denotes of
T105 181-188 NN denotes disease
T106 189-197 NN denotes severity
T107 198-201 CC denotes and
T108 202-214 RB denotes consequently
T109 214-215 -COMMA- denotes ,
T110 216-219 DT denotes the
T111 220-227 NN denotes quality
T112 228-230 IN denotes of
T113 231-235 NN denotes life
T114 236-239 CC denotes and
T115 240-247 NN denotes outcome
T116 248-251 IN denotes for
T117 252-260 NN denotes patients
T118 261-265 IN denotes with
T119 266-272 JJ denotes cystic
T120 273-281 NN denotes fibrosis
T121 282-283 -LRB- denotes (
T122 283-285 NN denotes CF
T123 285-286 -RRB- denotes )
T124 288-301 NN denotes Interleukin-1
T125 302-306 NN denotes beta
T126 307-308 -LRB- denotes (
T127 308-313 NN denotes IL-1β
T128 313-314 -RRB- denotes )
T129 315-317 VB denotes is
T130 318-319 DT denotes a
T131 320-323 JJ denotes key
T132 324-336 JJ denotes inflammatory
T133 337-345 NN denotes mediator
T134 347-356 NN denotes Secretion
T135 357-359 IN denotes of
T136 360-372 RB denotes biologically
T137 373-379 JJ denotes active
T138 380-385 NN denotes IL-1β
T139 386-394 VB denotes involves
T140 395-416 JJ denotes inflammasome-mediated
T141 417-427 NN denotes processing
T142 429-435 JJ denotes Little
T143 436-438 VB denotes is
T144 439-444 VB denotes known
T145 445-450 IN denotes about
T146 451-454 DT denotes the
T147 455-467 NN denotes contribution
T148 468-470 IN denotes of
T149 471-476 NN denotes IL-1β
T150 477-480 CC denotes and
T151 481-484 DT denotes the
T152 485-498 NN denotes inflammasomes
T153 499-501 IN denotes in
T154 502-504 NN denotes CF
T155 505-517 JJ denotes inflammatory
T156 518-525 NN denotes disease
T157 527-531 DT denotes This
T158 532-537 NN denotes study
T159 538-546 VB denotes examines
T160 547-568 JJ denotes inflammasome-mediated
T161 569-574 NN denotes IL-1β
T162 575-585 NN denotes production
T163 586-588 IN denotes in
T164 589-591 NN denotes CF
T165 592-601 JJ denotes bronchial
T166 602-612 JJ denotes epithelial
T167 613-617 NN denotes cell
T168 618-623 NN denotes lines
T169 624-627 CC denotes and
T170 628-633 JJ denotes human
T171 634-642 NN denotes patients
T172 643-647 IN denotes with
T173 648-650 NN denotes CF
T174 653-660 NN denotes Results
T175 661-670 JJ denotes Bronchial
T176 671-681 JJ denotes epithelial
T177 682-686 NN denotes cell
T178 687-692 NN denotes lines
T179 693-697 VB denotes were
T180 698-703 VB denotes found
T181 704-706 TO denotes to
T182 707-714 VB denotes produce
T183 715-725 JJ denotes negligible
T184 726-733 NN denotes amounts
T185 734-736 IN denotes of
T186 737-742 JJ denotes basal
T187 743-745 CC denotes or
T188 746-756 VB denotes stimulated
T189 757-762 NN denotes IL-1β
T190 763-771 VB denotes compared
T191 772-774 TO denotes to
T192 775-788 JJ denotes hematopoeitic
T193 789-794 NN denotes cells
T194 795-798 CC denotes and
T195 799-803 PRP denotes they
T196 804-807 VB denotes did
T197 808-811 RB denotes not
T198 812-825 RB denotes significantly
T199 826-836 VB denotes upregulate
T200 837-846 NN denotes caspase-1
T201 847-855 NN denotes activity
T202 856-860 IN denotes upon
T203 861-873 JJ denotes inflammasome
T204 874-885 NN denotes stimulation
T205 887-889 IN denotes In
T206 890-898 NN denotes contrast
T207 898-899 -COMMA- denotes ,
T208 900-910 JJ denotes peripheral
T209 911-916 NN denotes blood
T210 917-928 JJ denotes mononuclear
T211 929-934 NN denotes cells
T212 935-936 -LRB- denotes (
T213 936-941 NN denotes PBMCs
T214 941-942 -RRB- denotes )
T215 943-947 IN denotes from
T216 948-952 CC denotes both
T217 953-955 NN denotes CF
T218 956-959 CC denotes and
T219 960-967 JJ denotes healthy
T220 968-975 NN denotes control
T221 976-984 NN denotes subjects
T222 985-993 VB denotes produced
T223 994-999 JJ denotes large
T224 1000-1007 NN denotes amounts
T225 1008-1010 IN denotes of
T226 1011-1016 NN denotes IL-1β
T227 1017-1020 CC denotes and
T228 1021-1029 RB denotes strongly
T229 1030-1041 VB denotes upregulated
T230 1042-1051 NN denotes caspase-1
T231 1052-1060 NN denotes activity
T232 1061-1065 IN denotes upon
T233 1066-1078 JJ denotes inflammasome
T234 1079-1090 NN denotes stimulation
T235 1092-1097 NN denotes PBMCs
T236 1098-1102 IN denotes from
T237 1103-1105 NN denotes CF
T238 1106-1114 NN denotes patients
T239 1115-1118 CC denotes and
T240 1119-1127 NN denotes controls
T241 1128-1137 VB denotes displayed
T242 1138-1145 JJ denotes similar
T243 1146-1152 NN denotes levels
T244 1153-1155 IN denotes of
T245 1156-1165 NN denotes caspase-1
T246 1166-1176 NN denotes activation
T247 1177-1180 CC denotes and
T248 1181-1186 NN denotes IL-1β
T249 1187-1197 NN denotes production
T250 1198-1202 WRB denotes when
T251 1203-1213 VB denotes stimulated
T252 1214-1218 IN denotes with
T253 1219-1231 JJ denotes inflammasome
T254 1232-1242 NN denotes activators
T255 1244-1248 DT denotes This
T256 1249-1254 NN denotes IL-1β
T257 1255-1265 NN denotes production
T258 1266-1269 VB denotes was
T259 1270-1279 JJ denotes dependent
T260 1280-1282 IN denotes on
T261 1283-1288 NN denotes NF-κB
T262 1289-1297 NN denotes activity
T263 1298-1301 CC denotes and
T264 1302-1307 MD denotes could
T265 1308-1310 VB denotes be
T266 1311-1319 VB denotes enhanced
T267 1320-1322 IN denotes by
T268 1323-1330 VB denotes priming
T269 1331-1335 IN denotes with
T270 1336-1339 NN denotes LPS
T271 1341-1348 RB denotes Finally
T272 1348-1349 -COMMA- denotes ,
T273 1350-1358 JJ denotes chemical
T274 1359-1369 NN denotes inhibition
T275 1370-1372 IN denotes of
T276 1373-1377 NN denotes CFTR
T277 1378-1386 NN denotes activity
T278 1387-1389 IN denotes in
T279 1390-1397 NN denotes control
T280 1398-1403 NN denotes PBMCs
T281 1404-1407 CC denotes and
T282 1408-1413 NN denotes THP-1
T283 1414-1419 NN denotes cells
T284 1420-1423 VB denotes did
T285 1424-1427 RB denotes not
T286 1428-1441 RB denotes significantly
T287 1442-1447 VB denotes alter
T288 1448-1453 NN denotes IL-1β
T289 1454-1456 CC denotes or
T290 1457-1461 NN denotes IL-8
T291 1462-1472 NN denotes production
T292 1473-1475 IN denotes in
T293 1476-1484 NN denotes response
T294 1485-1487 TO denotes to
T295 1488-1490 NNP denotes P.
T296 1491-1501 NN denotes aeruginosa
T297 1504-1514 NN denotes Conclusion
T298 1515-1528 JJ denotes Hematopoeitic
T299 1529-1534 NN denotes cells
T300 1535-1541 VB denotes appear
T301 1542-1544 TO denotes to
T302 1545-1547 VB denotes be
T303 1548-1551 DT denotes the
T304 1552-1563 JJ denotes predominant
T305 1564-1570 NN denotes source
T306 1571-1573 IN denotes of
T307 1574-1594 JJ denotes inflammasome-induced
T308 1595-1611 JJ denotes pro-inflammatory
T309 1612-1617 NN denotes IL-1β
T310 1618-1620 IN denotes in
T311 1621-1623 NN denotes CF
T312 1625-1630 NN denotes PBMCs
T313 1631-1638 VB denotes derived
T314 1639-1643 IN denotes from
T315 1644-1646 NN denotes CF
T316 1647-1655 NN denotes subjects
T317 1656-1663 VB denotes display
T318 1664-1673 VB denotes preserved
T319 1674-1686 JJ denotes inflammasome
T320 1687-1697 NN denotes activation
T321 1698-1701 CC denotes and
T322 1702-1707 NN denotes IL-1β
T323 1708-1717 NN denotes secretion
T324 1718-1720 IN denotes in
T325 1721-1729 NN denotes response
T326 1730-1732 TO denotes to
T327 1733-1736 DT denotes the
T328 1737-1742 JJ denotes major
T329 1743-1745 NN denotes CF
T330 1746-1754 NN denotes pathogen
T331 1755-1766 NN denotes Pseudomonas
T332 1767-1777 NN denotes aeruginosa
T333 1779-1786 RB denotes However
T334 1786-1787 -COMMA- denotes ,
T335 1788-1791 PRP-DOLLAR- denotes our
T336 1792-1796 NN denotes data
T337 1797-1799 VB denotes do
T338 1800-1803 RB denotes not
T339 1804-1811 VB denotes support
T340 1812-1815 DT denotes the
T341 1816-1826 NN denotes hypothesis
T342 1827-1831 IN denotes that
T343 1832-1841 VB denotes increased
T344 1842-1847 NN denotes IL-1β
T345 1848-1858 NN denotes production
T346 1859-1861 IN denotes in
T347 1862-1864 NN denotes CF
T348 1865-1873 NN denotes subjects
T349 1874-1876 VB denotes is
T350 1877-1880 JJ denotes due
T351 1881-1883 TO denotes to
T352 1884-1886 DT denotes an
T353 1887-1896 JJ denotes intrinsic
T354 1897-1905 NN denotes increase
T355 1906-1908 IN denotes in
T356 1909-1914 NN denotes NF-κB
T357 1915-1923 NN denotes activity
T358 1924-1931 IN denotes through
T359 1932-1936 NN denotes loss
T360 1937-1939 IN denotes of
T361 1940-1944 NN denotes CFTR
T362 1945-1953 NN denotes function
T952 1970-1976 JJ denotes Cystic
T953 1977-1985 NN denotes fibrosis
T954 1986-1988 VB denotes is
T955 1989-1991 DT denotes an
T956 1992-2001 JJ denotes autosomal
T957 2002-2011 JJ denotes recessive
T958 2012-2019 NN denotes disease
T959 2020-2026 VB denotes caused
T960 2027-2029 IN denotes by
T961 2030-2039 NN denotes mutations
T962 2040-2042 IN denotes in
T963 2043-2046 DT denotes the
T964 2047-2053 JJ denotes cystic
T965 2054-2062 NN denotes fibrosis
T966 2063-2076 JJ denotes transmembrane
T967 2077-2088 NN denotes conductance
T968 2089-2098 NN denotes regulator
T969 2099-2100 -LRB- denotes (
T970 2100-2104 NN denotes CFTR
T971 2104-2105 -RRB- denotes )
T972 2105-2106 -COMMA- denotes ,
T973 2107-2112 WDT denotes which
T974 2113-2122 VB denotes functions
T975 2123-2125 IN denotes as
T976 2126-2127 DT denotes a
T977 2128-2136 NN denotes chloride
T978 2137-2140 NN denotes ion
T979 2141-2148 NN denotes channel
T980 2150-2152 NN denotes CF
T981 2153-2160 VB denotes remains
T982 2161-2164 CD denotes one
T983 2165-2167 IN denotes of
T984 2168-2171 DT denotes the
T985 2172-2176 RB denotes most
T986 2177-2183 JJ denotes common
T987 2184-2190 JJ denotes lethal
T988 2191-2198 JJ denotes genetic
T989 2199-2207 NN denotes diseases
T990 2208-2210 IN denotes in
T991 2211-2222 NN denotes populations
T992 2223-2225 IN denotes of
T993 2226-2234 JJ denotes European
T994 2235-2242 NN denotes descent
T995 2243-2247 IN denotes with
T996 2248-2251 DT denotes the
T997 2252-2259 JJ denotes current
T998 2260-2267 JJ denotes average
T999 2268-2276 NN denotes lifespan
T1000 2277-2279 IN denotes of
T1001 2280-2282 NN denotes CF
T1002 2283-2291 NN denotes patients
T1003 2292-2305 RB denotes approximately
T1004 2306-2308 CD denotes 40
T1005 2309-2314 NN denotes years
T1006 2315-2317 IN denotes of
T1007 2318-2321 NN denotes age
T1008 2322-2323 -LRB- denotes [
T1009 2323-2324 CD denotes 1
T1010 2324-2325 -RRB- denotes ]
T1011 2325-2326 -COMMA- denotes ,
T1012 2327-2328 -LRB- denotes [
T1013 2328-2329 CD denotes 2
T1014 2329-2330 -RRB- denotes ]
T1015 2332-2341 JJ denotes Recurrent
T1016 2342-2354 JJ denotes inflammatory
T1017 2355-2364 JJ denotes pulmonary
T1018 2365-2377 NN denotes exacerbation
T1019 2378-2380 VB denotes is
T1020 2381-2384 DT denotes the
T1021 2385-2392 JJ denotes primary
T1022 2393-2398 NN denotes cause
T1023 2399-2401 IN denotes of
T1024 2402-2406 NN denotes lung
T1025 2407-2414 NN denotes disease
T1026 2415-2426 NN denotes progression
T1027 2427-2430 CC denotes and
T1028 2431-2441 RB denotes ultimately
T1029 2441-2442 -COMMA- denotes ,
T1030 2443-2448 NN denotes death
T1031 2449-2451 IN denotes in
T1032 2452-2454 NN denotes CF
T1033 2456-2466 VB denotes Controlled
T1034 2467-2479 NN denotes inflammation
T1035 2480-2482 VB denotes is
T1036 2483-2492 JJ denotes important
T1037 2493-2496 IN denotes for
T1038 2497-2505 VB denotes fighting
T1039 2506-2515 NN denotes infection
T1040 2515-2516 -COMMA- denotes ,
T1041 2517-2520 CC denotes but
T1042 2521-2523 IN denotes in
T1043 2524-2530 NN denotes excess
T1044 2530-2531 -COMMA- denotes ,
T1045 2532-2534 PRP denotes it
T1046 2535-2542 VB denotes becomes
T1047 2543-2554 JJ denotes destructive
T1048 2555-2557 TO denotes to
T1049 2558-2562 NN denotes host
T1050 2563-2568 NN denotes cells
T1051 2569-2572 CC denotes and
T1052 2573-2575 TO denotes to
T1053 2576-2579 DT denotes the
T1054 2580-2592 NN denotes architecture
T1055 2593-2595 IN denotes of
T1056 2596-2599 DT denotes the
T1057 2600-2605 NN denotes lungs
T1058 2606-2607 -LRB- denotes [
T1059 2607-2608 CD denotes 2
T1060 2608-2609 -RRB- denotes ]
T1061 2609-2610 -COMMA- denotes ,
T1062 2611-2612 -LRB- denotes [
T1063 2612-2613 CD denotes 3
T1064 2613-2614 -RRB- denotes ]
T1065 2616-2618 IN denotes In
T1066 2619-2621 NN denotes CF
T1067 2621-2622 -COMMA- denotes ,
T1068 2623-2629 NN denotes airway
T1069 2630-2640 JJ denotes epithelial
T1070 2641-2646 NN denotes cells
T1071 2647-2651 VB denotes have
T1072 2652-2656 VB denotes been
T1073 2657-2662 VB denotes shown
T1074 2663-2665 TO denotes to
T1075 2666-2673 VB denotes produce
T1076 2674-2676 DT denotes an
T1077 2677-2688 JJ denotes exaggerated
T1078 2689-2705 JJ denotes pro-inflammatory
T1079 2706-2714 NN denotes cytokine
T1080 2715-2723 NN denotes response
T1081 2724-2726 TO denotes to
T1082 2727-2738 NN denotes stimulation
T1083 2739-2740 -LRB- denotes [
T1084 2740-2741 CD denotes 4
T1085 2741-2742 -RRB- denotes ]
T1086 2742-2743 -COMMA- denotes ,
T1087 2744-2745 -LRB- denotes [
T1088 2745-2746 CD denotes 5
T1089 2746-2747 -RRB- denotes ]
T1090 2749-2751 PRP denotes It
T1091 2752-2754 VB denotes is
T1092 2755-2762 JJ denotes unclear
T1093 2763-2770 IN denotes whether
T1094 2771-2775 DT denotes this
T1095 2776-2786 VB denotes heightened
T1096 2787-2799 JJ denotes inflammatory
T1097 2800-2808 NN denotes response
T1098 2809-2811 VB denotes is
T1099 2812-2821 JJ denotes intrinsic
T1100 2822-2824 TO denotes to
T1101 2825-2830 NN denotes cells
T1102 2831-2838 VB denotes lacking
T1103 2839-2843 NN denotes CFTR
T1104 2844-2846 CC denotes or
T1105 2847-2854 IN denotes whether
T1106 2855-2857 PRP denotes it
T1107 2858-2860 VB denotes is
T1108 2861-2862 DT denotes a
T1109 2863-2869 NN denotes result
T1110 2870-2872 IN denotes of
T1111 2873-2880 JJ denotes chronic
T1112 2881-2894 JJ denotes polymicrobial
T1113 2895-2904 NN denotes infection
T1114 2905-2906 -LRB- denotes [
T1115 2906-2907 CD denotes 6
T1116 2907-2908 -RRB- denotes ]
T1117 2908-2909 -COMMA- denotes ,
T1118 2910-2911 -LRB- denotes [
T1119 2911-2912 CD denotes 7
T1120 2912-2913 -RRB- denotes ]
T1121 2915-2925 RB denotes Regardless
T1122 2926-2928 IN denotes of
T1123 2929-2933 DT denotes this
T1124 2934-2945 NN denotes controversy
T1125 2945-2946 -COMMA- denotes ,
T1126 2947-2958 VB denotes identifying
T1127 2959-2962 CC denotes and
T1128 2963-2972 VB denotes targeting
T1129 2973-2981 JJ denotes relevant
T1130 2982-2994 JJ denotes inflammatory
T1131 2995-3004 NN denotes mediators
T1132 3005-3007 VB denotes is
T1133 3008-3009 DT denotes a
T1134 3010-3018 JJ denotes critical
T1135 3019-3023 NN denotes step
T1136 3024-3026 IN denotes in
T1137 3027-3037 VB denotes developing
T1138 3038-3042 RB denotes more
T1139 3043-3051 JJ denotes specific
T1140 3052-3063 JJ denotes therapeutic
T1141 3064-3074 NN denotes approaches
T1142 3075-3077 TO denotes to
T1143 3078-3085 VB denotes control
T1144 3086-3098 NN denotes inflammation
T1145 3099-3102 CC denotes and
T1146 3103-3110 VB denotes improve
T1147 3111-3117 NN denotes health
T1148 3118-3126 NN denotes outcomes
T1149 3127-3129 IN denotes in
T1150 3130-3132 NN denotes CF
T1151 3133-3134 -LRB- denotes [
T1152 3134-3135 CD denotes 8
T1153 3135-3136 -RRB- denotes ]
T1154 3138-3151 NN denotes Interleukin-1
T1155 3152-3156 NN denotes beta
T1156 3157-3158 -LRB- denotes (
T1157 3158-3163 NN denotes IL-1β
T1158 3163-3164 -RRB- denotes )
T1159 3165-3167 VB denotes is
T1160 3168-3169 DT denotes a
T1161 3170-3175 JJ denotes major
T1162 3176-3188 JJ denotes inflammatory
T1163 3189-3197 NN denotes mediator
T1164 3199-3202 PRP-DOLLAR- denotes Its
T1165 3203-3216 JJ denotes physiological
T1166 3217-3224 NN denotes effects
T1167 3225-3228 VB denotes are
T1168 3229-3236 JJ denotes diverse
T1169 3237-3240 CC denotes and
T1170 3241-3252 RB denotes potentially
T1171 3253-3262 JJ denotes important
T1172 3263-3265 TO denotes to
T1173 3266-3269 DT denotes the
T1174 3270-3282 NN denotes pathogenesis
T1175 3283-3285 IN denotes of
T1176 3286-3290 NN denotes lung
T1177 3291-3304 NN denotes exacerbations
T1178 3305-3307 IN denotes in
T1179 3308-3310 NN denotes CF
T1180 3310-3311 -COMMA- denotes ,
T1181 3312-3321 VB denotes including
T1182 3322-3325 DT denotes the
T1183 3326-3336 NN denotes generation
T1184 3337-3339 IN denotes of
T1185 3340-3345 NN denotes fever
T1186 3345-3346 -COMMA- denotes ,
T1187 3347-3350 DT denotes the
T1188 3351-3362 NN denotes recruitment
T1189 3363-3365 IN denotes of
T1190 3366-3378 JJ denotes inflammatory
T1191 3379-3387 NN denotes effector
T1192 3388-3393 NN denotes cells
T1193 3393-3394 -COMMA- denotes ,
T1194 3395-3398 DT denotes the
T1195 3399-3408 NN denotes induction
T1196 3409-3411 IN denotes of
T1197 3412-3417 JJ denotes other
T1198 3418-3434 JJ denotes pro-inflammatory
T1199 3435-3444 NN denotes cytokines
T1200 3445-3449 JJ denotes such
T1201 3450-3452 IN denotes as
T1202 3453-3457 NN denotes IL-6
T1203 3458-3461 CC denotes and
T1204 3462-3466 NN denotes IL-8
T1205 3466-3467 -COMMA- denotes ,
T1206 3468-3471 CC denotes and
T1207 3472-3475 DT denotes the
T1208 3476-3483 NN denotes shaping
T1209 3484-3486 IN denotes of
T1210 3487-3488 NN denotes T
T1211 3489-3493 NN denotes cell
T1212 3494-3503 NN denotes responses
T1213 3504-3505 -LRB- denotes [
T1214 3505-3506 CD denotes 9
T1215 3506-3507 -RRB- denotes ]
T1216 3507-3508 -COMMA- denotes ,
T1217 3509-3510 -LRB- denotes [
T1218 3510-3512 CD denotes 10
T1219 3512-3513 -RRB- denotes ]
T1220 3515-3524 VB denotes Following
T1221 3525-3535 NN denotes initiation
T1222 3536-3538 IN denotes of
T1223 3539-3542 DT denotes the
T1224 3543-3548 NN denotes NF-κB
T1225 3549-3558 NN denotes signaling
T1226 3559-3566 NN denotes cascade
T1227 3566-3567 -COMMA- denotes ,
T1228 3568-3573 NN denotes IL-1β
T1229 3574-3576 VB denotes is
T1230 3577-3585 VB denotes produced
T1231 3586-3588 IN denotes in
T1232 3589-3592 DT denotes the
T1233 3593-3600 NN denotes cytosol
T1234 3601-3603 IN denotes as
T1235 3604-3605 DT denotes a
T1236 3606-3618 RB denotes biologically
T1237 3619-3627 JJ denotes inactive
T1238 3628-3639 JJ denotes full-length
T1239 3640-3649 NN denotes pro-IL-1β
T1240 3651-3660 NN denotes Pro-IL-1β
T1241 3661-3663 VB denotes is
T1242 3664-3676 RB denotes subsequently
T1243 3677-3686 VB denotes converted
T1244 3687-3691 IN denotes into
T1245 3692-3695 PRP-DOLLAR- denotes its
T1246 3696-3702 JJ denotes active
T1247 3703-3707 NN denotes form
T1248 3708-3710 IN denotes by
T1249 3711-3720 JJ denotes cytosolic
T1250 3721-3728 NN denotes protein
T1251 3729-3738 NN denotes complexes
T1252 3739-3745 VB denotes termed
T1253 3746-3762 NN denotes “inflammasomes.”
T1254 3763-3776 NN denotes Inflammasomes
T1255 3777-3785 NN denotes assemble
T1256 3786-3788 IN denotes in
T1257 3789-3797 NN denotes response
T1258 3798-3800 TO denotes to
T1259 3801-3808 JJ denotes certain
T1260 3809-3817 JJ denotes cellular
T1261 3818-3824 NN denotes danger
T1262 3825-3832 NN denotes signals
T1263 3833-3836 CC denotes and
T1264 3837-3844 VB denotes mediate
T1265 3845-3848 DT denotes the
T1266 3849-3864 NN denotes auto-activation
T1267 3865-3867 IN denotes of
T1268 3868-3877 NN denotes caspase-1
T1269 3878-3879 -LRB- denotes [
T1270 3879-3880 CD denotes 9
T1271 3880-3881 -RRB- denotes ]
T1272 3881-3882 -COMMA- denotes ,
T1273 3883-3884 -LRB- denotes [
T1274 3884-3886 CD denotes 11
T1275 3886-3887 -RRB- denotes ]
T1276 3887-3888 -COMMA- denotes ,
T1277 3889-3894 WDT denotes which
T1278 3895-3902 VB denotes cleaves
T1279 3903-3912 NN denotes pro-IL-1β
T1280 3913-3916 CC denotes and
T1281 3917-3926 NN denotes pro-IL-18
T1282 3927-3931 IN denotes into
T1283 3932-3937 PRP-DOLLAR- denotes their
T1284 3938-3950 RB denotes biologically
T1285 3951-3957 JJ denotes active
T1286 3958-3963 NN denotes forms
T1287 3964-3967 IN denotes for
T1288 3968-3977 NN denotes secretion
T1289 3979-3983 CD denotes Four
T1290 3984-3992 JJ denotes distinct
T1291 3993-4006 NN denotes inflammasomes
T1292 4007-4011 VB denotes have
T1293 4012-4016 VB denotes been
T1294 4017-4027 VB denotes recognized
T1295 4029-4034 DT denotes These
T1296 4035-4038 VB denotes are
T1297 4039-4042 DT denotes the
T1298 4043-4048 NN denotes NLRP1
T1299 4049-4050 -LRB- denotes [
T1300 4050-4052 CD denotes 12
T1301 4052-4053 -RRB- denotes ]
T1302 4053-4054 -COMMA- denotes ,
T1303 4055-4060 NN denotes NLRP3
T1304 4061-4062 -LRB- denotes [
T1305 4062-4064 CD denotes 13
T1306 4064-4065 -RRB- denotes ]
T1307 4065-4066 -COMMA- denotes ,
T1308 4067-4068 -LRB- denotes [
T1309 4068-4070 CD denotes 14
T1310 4070-4071 -RRB- denotes ]
T1311 4071-4072 -COMMA- denotes ,
T1312 4073-4078 NN denotes NLRC4
T1313 4079-4080 -LRB- denotes [
T1314 4080-4082 CD denotes 15
T1315 4082-4083 -RRB- denotes ]
T1316 4083-4084 -COMMA- denotes ,
T1317 4085-4086 -LRB- denotes [
T1318 4086-4088 CD denotes 16
T1319 4088-4089 -RRB- denotes ]
T1320 4089-4090 -COMMA- denotes ,
T1321 4091-4094 CC denotes and
T1322 4095-4099 NN denotes AIM2
T1323 4100-4113 NN denotes inflammasomes
T1324 4114-4115 -LRB- denotes [
T1325 4115-4117 CD denotes 17
T1326 4117-4118 -RRB- denotes ]
T1327 4118-4119 -COMMA- denotes ,
T1328 4120-4121 -LRB- denotes [
T1329 4121-4123 CD denotes 18
T1330 4123-4124 -RRB- denotes ]
T1331 4124-4125 -COMMA- denotes ,
T1332 4126-4131 WDT denotes which
T1333 4132-4139 VB denotes respond
T1334 4140-4142 TO denotes to
T1335 4143-4144 DT denotes a
T1336 4145-4152 NN denotes variety
T1337 4153-4155 IN denotes of
T1338 4156-4165 JJ denotes different
T1339 4166-4175 JJ denotes microbial
T1340 4176-4186 NN denotes signatures
T1341 4187-4190 CC denotes and
T1342 4191-4197 NN denotes danger
T1343 4198-4205 NN denotes signals
T1344 4206-4207 -LRB- denotes [
T1345 4207-4209 CD denotes 11
T1346 4209-4210 -RRB- denotes ]
T1347 4212-4214 NNP denotes P.
T1348 4215-4225 NNP denotes aeruginosa
T1349 4225-4226 -COMMA- denotes ,
T1350 4227-4230 CD denotes one
T1351 4231-4233 IN denotes of
T1352 4234-4237 DT denotes the
T1353 4238-4242 RB denotes most
T1354 4243-4249 JJ denotes common
T1355 4250-4253 CC denotes and
T1356 4254-4264 RB denotes clinically
T1357 4265-4273 JJ denotes relevant
T1358 4274-4283 NN denotes pathogens
T1359 4284-4289 IN denotes among
T1360 4290-4292 NN denotes CF
T1361 4293-4301 NN denotes patients
T1362 4301-4302 -COMMA- denotes ,
T1363 4303-4312 VB denotes activates
T1364 4313-4316 DT denotes the
T1365 4317-4322 NN denotes NLRC4
T1366 4323-4335 NN denotes inflammasome
T1367 4336-4337 -LRB- denotes [
T1368 4337-4339 CD denotes 19
T1369 4339-4340 -RRB- denotes ]
T1370 4340-4341 -COMMA- denotes ,
T1371 4342-4343 -LRB- denotes [
T1372 4343-4345 CD denotes 20
T1373 4345-4346 -RRB- denotes ]
T1374 4348-4357 NN denotes Infection
T1375 4358-4362 IN denotes with
T1376 4363-4365 NNP denotes P.
T1377 4366-4376 NN denotes aeruginosa
T1378 4377-4385 VB denotes triggers
T1379 4386-4388 DT denotes an
T1380 4389-4397 NN denotes increase
T1381 4398-4400 IN denotes in
T1382 4401-4407 NN denotes levels
T1383 4408-4410 IN denotes of
T1384 4411-4416 NN denotes IL-1β
T1385 4416-4417 -COMMA- denotes ,
T1386 4418-4422 NN denotes IL-6
T1387 4422-4423 -COMMA- denotes ,
T1388 4424-4427 CC denotes and
T1389 4428-4432 NN denotes IL-8
T1390 4433-4435 IN denotes in
T1391 4436-4451 JJ denotes bronchoalveolar
T1392 4452-4458 NN denotes lavage
T1393 4459-4464 NN denotes fluid
T1394 4465-4466 -LRB- denotes (
T1395 4466-4470 NN denotes BALF
T1396 4470-4471 -RRB- denotes )
T1397 4472-4476 IN denotes from
T1398 4477-4485 NN denotes patients
T1399 4486-4490 IN denotes with
T1400 4491-4493 NN denotes CF
T1401 4494-4495 -LRB- denotes [
T1402 4495-4497 CD denotes 21
T1403 4497-4498 -RRB- denotes ]
T1404 4500-4512 JJ denotes Inflammasome
T1405 4513-4522 NN denotes responses
T1406 4523-4529 VB denotes depend
T1407 4530-4532 IN denotes on
T1408 4533-4538 NN denotes NF-κB
T1409 4539-4548 NN denotes signaling
T1410 4548-4549 -COMMA- denotes ,
T1411 4550-4555 WRB denotes where
T1412 4556-4561 NN denotes NF-κB
T1413 4562-4564 VB denotes is
T1414 4565-4574 JJ denotes important
T1415 4575-4577 IN denotes in
T1416 4578-4582 CC denotes both
T1417 4583-4586 DT denotes the
T1418 4587-4599 NN denotes upregulation
T1419 4600-4602 IN denotes of
T1420 4603-4611 JJ denotes specific
T1421 4612-4624 JJ denotes inflammasome
T1422 4625-4635 NN denotes components
T1423 4636-4637 -LRB- denotes [
T1424 4637-4639 CD denotes 22
T1425 4639-4640 -RRB- denotes ]
T1426 4640-4641 -COMMA- denotes ,
T1427 4642-4643 -LRB- denotes [
T1428 4643-4645 CD denotes 23
T1429 4645-4646 -RRB- denotes ]
T1430 4646-4647 -COMMA- denotes ,
T1431 4648-4650 RB denotes as
T1432 4651-4655 RB denotes well
T1433 4656-4658 IN denotes as
T1434 4659-4664 NN denotes IL-1β
T1435 4665-4675 NN denotes expression
T1436 4676-4677 -LRB- denotes [
T1437 4677-4679 CD denotes 24
T1438 4679-4680 -RRB- denotes ]
T1439 4680-4681 -COMMA- denotes ,
T1440 4682-4683 -LRB- denotes [
T1441 4683-4685 CD denotes 25
T1442 4685-4686 -RRB- denotes ]
T1443 4688-4696 JJ denotes Previous
T1444 4697-4704 NN denotes studies
T1445 4705-4712 VB denotes support
T1446 4713-4714 DT denotes a
T1447 4715-4719 NN denotes role
T1448 4720-4723 IN denotes for
T1449 4724-4729 NN denotes IL-1β
T1450 4730-4732 IN denotes in
T1451 4733-4736 DT denotes the
T1452 4737-4749 NN denotes pathogenesis
T1453 4750-4752 IN denotes of
T1454 4753-4755 NN denotes CF
T1455 4756-4768 JJ denotes inflammatory
T1456 4769-4773 NN denotes lung
T1457 4774-4781 NN denotes disease
T1458 4783-4789 NN denotes Levels
T1459 4790-4792 IN denotes of
T1460 4793-4798 NN denotes IL-1β
T1461 4799-4802 VB denotes are
T1462 4803-4812 VB denotes increased
T1463 4813-4815 IN denotes in
T1464 4816-4820 NN denotes BALF
T1465 4821-4825 IN denotes from
T1466 4826-4828 NN denotes CF
T1467 4829-4837 NN denotes patients
T1468 4838-4842 IN denotes with
T1469 4843-4852 NN denotes infection
T1470 4853-4854 -LRB- denotes [
T1471 4854-4856 CD denotes 21
T1472 4856-4857 -RRB- denotes ]
T1473 4857-4858 -COMMA- denotes ,
T1474 4859-4860 -LRB- denotes [
T1475 4860-4862 CD denotes 26
T1476 4862-4863 -RRB- denotes ]
T1477 4863-4864 -COMMA- denotes ,
T1478 4865-4866 -LRB- denotes [
T1479 4866-4868 CD denotes 27
T1480 4868-4869 -RRB- denotes ]
T1481 4869-4870 -COMMA- denotes ,
T1482 4871-4872 -LRB- denotes [
T1483 4872-4874 CD denotes 28
T1484 4874-4875 -RRB- denotes ]
T1485 4876-4879 CC denotes and
T1486 4880-4884 DT denotes this
T1487 4885-4893 NN denotes increase
T1488 4894-4897 VB denotes has
T1489 4898-4902 VB denotes been
T1490 4903-4913 RB denotes temporally
T1491 4914-4924 VB denotes associated
T1492 4925-4929 IN denotes with
T1493 4930-4931 DT denotes a
T1494 4932-4940 JJ denotes clinical
T1495 4941-4949 NN denotes response
T1496 4950-4952 TO denotes to
T1497 4953-4962 NN denotes treatment
T1498 4963-4964 -LRB- denotes [
T1499 4964-4966 CD denotes 21
T1500 4966-4967 -RRB- denotes ]
T1501 4969-4982 NN denotes Polymorphisms
T1502 4983-4985 IN denotes in
T1503 4986-4989 DT denotes the
T1504 4990-4994 NN denotes IL1B
T1505 4995-4999 NN denotes gene
T1506 5000-5004 VB denotes have
T1507 5005-5009 RB denotes also
T1508 5010-5014 VB denotes been
T1509 5015-5025 VB denotes associated
T1510 5026-5030 IN denotes with
T1511 5031-5038 VB denotes varying
T1512 5039-5046 NN denotes degrees
T1513 5047-5049 IN denotes of
T1514 5050-5057 NN denotes disease
T1515 5058-5066 NN denotes severity
T1516 5067-5069 IN denotes in
T1517 5070-5072 NN denotes CF
T1518 5073-5081 NN denotes patients
T1519 5082-5083 -LRB- denotes [
T1520 5083-5085 CD denotes 29
T1521 5085-5086 -RRB- denotes ]
T1522 5088-5094 JJ denotes Murine
T1523 5095-5101 NN denotes models
T1524 5102-5104 IN denotes of
T1525 5105-5109 NN denotes CFTR
T1526 5110-5121 NN denotes dysfunction
T1527 5122-5126 VB denotes have
T1528 5127-5136 VB denotes exhibited
T1529 5137-5148 JJ denotes significant
T1530 5149-5158 NN denotes increases
T1531 5159-5161 IN denotes in
T1532 5162-5167 NN denotes IL-1β
T1533 5168-5178 NN denotes expression
T1534 5179-5181 CC denotes or
T1535 5182-5191 NN denotes secretion
T1536 5192-5194 IN denotes in
T1537 5195-5206 NN denotes macrophages
T1538 5207-5208 -LRB- denotes [
T1539 5208-5210 CD denotes 30
T1540 5210-5211 -RRB- denotes ]
T1541 5211-5212 -COMMA- denotes ,
T1542 5213-5214 -LRB- denotes [
T1543 5214-5216 CD denotes 31
T1544 5216-5217 -RRB- denotes ]
T1545 5217-5218 -COMMA- denotes ,
T1546 5219-5222 CC denotes and
T1547 5223-5230 VB denotes support
T1548 5231-5234 DT denotes the
T1549 5235-5245 NN denotes hypothesis
T1550 5246-5250 IN denotes that
T1551 5251-5254 DT denotes the
T1552 5255-5259 NN denotes loss
T1553 5260-5262 IN denotes of
T1554 5263-5267 NN denotes CFTR
T1555 5268-5277 VB denotes increases
T1556 5278-5283 NN denotes NF-κB
T1557 5284-5294 NN denotes activation
T1558 5295-5300 IN denotes under
T1559 5301-5306 JJ denotes basal
T1560 5307-5310 CC denotes and
T1561 5311-5322 JJ denotes stimulatory
T1562 5323-5333 NN denotes conditions
T1563 5334-5335 -LRB- denotes [
T1564 5335-5336 CD denotes 4
T1565 5336-5337 -RRB- denotes ]
T1566 5337-5338 -COMMA- denotes ,
T1567 5339-5340 -LRB- denotes [
T1568 5340-5341 CD denotes 5
T1569 5341-5342 -RRB- denotes ]
T1570 5342-5343 -COMMA- denotes ,
T1571 5344-5345 -LRB- denotes [
T1572 5345-5347 CD denotes 32
T1573 5347-5348 -RRB- denotes ]
T1574 5348-5349 -COMMA- denotes ,
T1575 5350-5351 -LRB- denotes [
T1576 5351-5353 CD denotes 33
T1577 5353-5354 -RRB- denotes ]
T1578 5356-5363 RB denotes Finally
T1579 5363-5364 -COMMA- denotes ,
T1580 5365-5376 NN denotes replacement
T1581 5377-5379 IN denotes of
T1582 5380-5388 NN denotes chloride
T1583 5389-5393 NN denotes ions
T1584 5394-5398 IN denotes with
T1585 5399-5408 NN denotes glutamate
T1586 5409-5411 CC denotes or
T1587 5412-5421 NN denotes gluconate
T1588 5422-5424 IN denotes in
T1589 5425-5429 NN denotes cell
T1590 5430-5437 NN denotes culture
T1591 5438-5443 NN denotes media
T1592 5444-5453 VB denotes increases
T1593 5454-5463 NN denotes secretion
T1594 5464-5466 IN denotes of
T1595 5467-5472 NN denotes IL-1β
T1596 5473-5475 IN denotes in
T1597 5476-5484 NN denotes response
T1598 5485-5487 TO denotes to
T1599 5488-5493 NN denotes NLRP3
T1600 5494-5505 NN denotes stimulation
T1601 5506-5508 IN denotes by
T1602 5509-5518 NN denotes adenosine
T1603 5519-5531 NN denotes triphosphate
T1604 5532-5533 -LRB- denotes (
T1605 5533-5536 NN denotes ATP
T1606 5536-5537 -RRB- denotes )
T1607 5538-5539 -LRB- denotes [
T1608 5539-5541 CD denotes 34
T1609 5541-5542 -RRB- denotes ]
T1610 5542-5543 -COMMA- denotes ,
T1611 5544-5552 VB denotes implying
T1612 5553-5555 DT denotes an
T1613 5556-5566 JJ denotes inhibitory
T1614 5567-5571 NN denotes role
T1615 5572-5575 IN denotes for
T1616 5576-5589 JJ denotes extracellular
T1617 5590-5598 NN denotes chloride
T1618 5599-5601 IN denotes in
T1619 5602-5607 NN denotes NLRP3
T1620 5608-5618 NN denotes activation
T1621 5620-5625 VB denotes Taken
T1622 5626-5634 RB denotes together
T1623 5634-5635 -COMMA- denotes ,
T1624 5636-5641 DT denotes these
T1625 5642-5646 NN denotes data
T1626 5647-5656 VB denotes implicate
T1627 5657-5660 DT denotes the
T1628 5661-5672 NN denotes involvement
T1629 5673-5675 IN denotes of
T1630 5676-5681 NN denotes IL-1β
T1631 5682-5685 CC denotes and
T1632 5686-5698 RB denotes consequently
T1633 5698-5699 -COMMA- denotes ,
T1634 5700-5703 DT denotes the
T1635 5704-5717 NN denotes inflammasomes
T1636 5717-5718 -COMMA- denotes ,
T1637 5719-5721 IN denotes in
T1638 5722-5724 NN denotes CF
T1639 5725-5737 JJ denotes inflammatory
T1640 5738-5745 NN denotes disease
T2611 5757-5763 RB denotes Airway
T2612 5764-5774 JJ denotes epithelial
T2613 5775-5780 NN denotes cells
T2614 5781-5783 VB denotes do
T2615 5784-5787 RB denotes not
T2616 5788-5795 VB denotes produce
T2617 5796-5807 JJ denotes significant
T2618 5808-5815 NN denotes amounts
T2619 5816-5818 IN denotes of
T2620 5819-5824 NN denotes IL-1β
T2621 5825-5827 IN denotes in
T2622 5828-5836 NN denotes response
T2623 5837-5839 TO denotes to
T2624 5840-5852 JJ denotes inflammasome
T2625 5853-5864 NN denotes stimulation
T2626 5865-5868 DT denotes The
T2627 5869-5882 NN denotes inflammasomes
T2628 5883-5886 CC denotes and
T2629 5887-5892 PRP-DOLLAR- denotes their
T2630 5893-5903 JJ denotes respective
T2631 5904-5914 NN denotes activators
T2632 5915-5923 VB denotes examined
T2633 5924-5926 IN denotes in
T2634 5927-5931 DT denotes this
T2635 5932-5937 NN denotes study
T2636 5938-5941 VB denotes are
T2637 5942-5948 VB denotes listed
T2638 5949-5951 IN denotes in
T2639 5952-5957 JJ denotes Table
T2640 5958-5959 CD denotes 1
T2641 5961-5966 NN denotes Cells
T2642 5967-5971 VB denotes were
T2643 5972-5982 VB denotes stimulated
T2644 5983-5985 IN denotes in
T2645 5986-5996 NN denotes accordance
T2646 5997-6001 IN denotes with
T2647 6002-6005 DT denotes the
T2648 6006-6014 NN denotes schedule
T2649 6015-6017 IN denotes in
T2650 6018-6024 NN denotes Figure
T2651 6025-6026 CD denotes 1
T2652 6028-6030 IN denotes In
T2653 6031-6033 NN denotes CF
T2654 6033-6034 -COMMA- denotes ,
T2655 6035-6041 NN denotes airway
T2656 6042-6052 JJ denotes epithelial
T2657 6053-6058 NN denotes cells
T2658 6059-6063 VB denotes have
T2659 6064-6068 VB denotes been
T2660 6069-6074 VB denotes shown
T2661 6075-6077 TO denotes to
T2662 6078-6085 VB denotes possess
T2663 6086-6087 DT denotes a
T2664 6088-6106 JJ denotes hyper-inflammatory
T2665 6107-6116 NN denotes phenotype
T2666 6117-6120 CC denotes and
T2667 6121-6128 VB denotes produce
T2668 6129-6131 DT denotes an
T2669 6132-6143 JJ denotes exaggerated
T2670 6144-6160 JJ denotes pro-inflammatory
T2671 6161-6169 NN denotes cytokine
T2672 6170-6178 NN denotes response
T2673 6179-6180 -LRB- denotes [
T2674 6180-6181 CD denotes 4
T2675 6181-6182 -RRB- denotes ]
T2676 6182-6183 -COMMA- denotes ,
T2677 6184-6185 -LRB- denotes [
T2678 6185-6186 CD denotes 5
T2679 6186-6187 -RRB- denotes ]
T2680 6189-6191 TO denotes To
T2681 6192-6201 VB denotes determine
T2682 6202-6204 IN denotes if
T2683 6205-6211 NN denotes airway
T2684 6212-6222 JJ denotes epithelial
T2685 6223-6228 NN denotes cells
T2686 6229-6239 VB denotes contribute
T2687 6240-6242 TO denotes to
T2688 6243-6246 DT denotes the
T2689 6247-6256 VB denotes increased
T2690 6257-6262 NN denotes IL-1β
T2691 6263-6273 NN denotes production
T2692 6274-6276 IN denotes in
T2693 6277-6285 NN denotes patients
T2694 6286-6290 IN denotes with
T2695 6291-6293 NN denotes CF
T2696 6293-6294 -COMMA- denotes ,
T2697 6295-6297 NN denotes CF
T2698 6298-6301 CC denotes and
T2699 6302-6309 NN denotes control
T2700 6310-6319 JJ denotes bronchial
T2701 6320-6330 JJ denotes epithelial
T2702 6331-6335 NN denotes cell
T2703 6336-6341 NN denotes lines
T2704 6342-6346 VB denotes were
T2705 6347-6357 VB denotes stimulated
T2706 6358-6362 IN denotes with
T2707 6363-6366 DT denotes the
T2708 6367-6379 JJ denotes inflammasome
T2709 6380-6388 NN denotes inducers
T2710 6389-6391 NNP denotes P.
T2711 6392-6402 NN denotes aeruginosa
T2712 6403-6409 NN denotes strain
T2713 6410-6414 NN denotes PAO1
T2714 6415-6416 -LRB- denotes (
T2715 6416-6420 NN denotes PAO1
T2716 6420-6421 -RRB- denotes )
T2717 6422-6425 CC denotes and
T2718 6426-6429 NN denotes LPS
T2719 6430-6438 VB denotes followed
T2720 6439-6441 IN denotes by
T2721 6442-6445 NN denotes ATP
T2722 6447-6452 NN denotes IL-1β
T2723 6453-6459 NN denotes levels
T2724 6460-6462 IN denotes in
T2725 6463-6467 NN denotes cell
T2726 6468-6475 NN denotes culture
T2727 6476-6488 NN denotes supernatants
T2728 6489-6493 VB denotes were
T2729 6494-6497 RB denotes not
T2730 6498-6505 RB denotes greatly
T2731 6506-6515 VB denotes increased
T2732 6516-6518 IN denotes in
T2733 6519-6525 CC denotes either
T2734 6526-6529 DT denotes the
T2735 6530-6532 NN denotes CF
T2736 6533-6535 CC denotes or
T2737 6536-6543 NN denotes control
T2738 6544-6548 NN denotes cell
T2739 6549-6554 NN denotes lines
T2740 6555-6556 -LRB- denotes (
T2741 6556-6560 NNP denotes Fig.
T2742 6561-6565 NN denotes 2a–d
T2743 6565-6566 -RRB- denotes )
T2744 6566-6567 -COMMA- denotes ,
T2745 6568-6576 IN denotes although
T2746 6577-6578 DT denotes a
T2747 6579-6584 JJ denotes small
T2748 6585-6593 NN denotes increase
T2749 6594-6596 IN denotes in
T2750 6597-6602 NN denotes IL-1β
T2751 6603-6613 NN denotes production
T2752 6614-6617 VB denotes was
T2753 6618-6626 VB denotes detected
T2754 6627-6629 IN denotes in
T2755 6630-6636 NN denotes NuLi-1
T2756 6637-6640 CC denotes and
T2757 6641-6647 NN denotes CuFi-1
T2758 6648-6653 NN denotes cells
T2759 6653-6654 -COMMA- denotes ,
T2760 6655-6658 CC denotes but
T2761 6659-6662 RB denotes not
T2762 6663-6665 IN denotes in
T2763 6666-6668 NN denotes S9
T2764 6669-6672 CC denotes and
T2765 6673-6678 NN denotes IB3-1
T2766 6679-6684 NN denotes cells
T2767 6684-6685 -COMMA- denotes ,
T2768 6686-6688 IN denotes by
T2769 6689-6691 CD denotes 24
T2770 6692-6697 NN denotes hours
T2771 6699-6701 IN denotes In
T2772 6702-6710 NN denotes contrast
T2773 6710-6711 -COMMA- denotes ,
T2774 6712-6717 DT denotes these
T2775 6718-6724 NN denotes airway
T2776 6725-6730 NN denotes cells
T2777 6731-6735 VB denotes were
T2778 6736-6742 RB denotes highly
T2779 6743-6753 JJ denotes responsive
T2780 6754-6756 TO denotes to
T2781 6757-6762 JJ denotes other
T2782 6763-6775 JJ denotes inflammatory
T2783 6776-6783 NN denotes stimuli
T2784 6783-6784 -COMMA- denotes ,
T2785 6785-6789 JJ denotes such
T2786 6790-6792 IN denotes as
T2787 6793-6804 JJ denotes recombinant
T2788 6805-6810 NN denotes IL-1β
T2789 6810-6811 -COMMA- denotes ,
T2790 6812-6821 VB denotes producing
T2791 6822-6827 JJ denotes large
T2792 6828-6838 NN denotes quantities
T2793 6839-6841 IN denotes of
T2794 6842-6846 NN denotes IL-8
T2795 6847-6848 -LRB- denotes (
T2796 6848-6852 NNP denotes Fig.
T2797 6853-6857 CD denotes 2a–d
T2798 6858-6865 NN denotes inserts
T2799 6865-6866 -RRB- denotes )
T3095 7936-7942 RB denotes Airway
T3096 7943-7953 JJ denotes epithelial
T3097 7954-7959 NN denotes cells
T3098 7960-7962 VB denotes do
T3099 7963-7966 RB denotes not
T3100 7967-7980 RB denotes significantly
T3101 7981-7991 VB denotes upregulate
T3102 7992-8001 NN denotes caspase-1
T3103 8002-8010 NN denotes activity
T3104 8011-8013 IN denotes in
T3105 8014-8022 NN denotes response
T3106 8023-8025 TO denotes to
T3107 8026-8038 JJ denotes inflammasome
T3108 8039-8050 NN denotes stimulation
T3109 8051-8053 TO denotes To
T3110 8054-8061 VB denotes examine
T3111 8062-8064 IN denotes if
T3112 8065-8077 JJ denotes inflammasome
T3113 8078-8088 NN denotes activation
T3114 8089-8095 VB denotes occurs
T3115 8096-8098 IN denotes in
T3116 8099-8104 DT denotes these
T3117 8105-8111 NN denotes airway
T3118 8112-8117 NN denotes cells
T3119 8117-8118 -COMMA- denotes ,
T3120 8119-8128 NN denotes caspase-1
T3121 8129-8137 NN denotes activity
T3122 8138-8141 VB denotes was
T3123 8142-8152 VB denotes quantified
T3124 8153-8155 IN denotes by
T3125 8156-8160 NN denotes flow
T3126 8161-8170 NN denotes cytometry
T3127 8172-8177 EX denotes There
T3128 8178-8181 VB denotes was
T3129 8182-8184 DT denotes no
T3130 8185-8196 JJ denotes significant
T3131 8197-8205 NN denotes increase
T3132 8206-8210 IN denotes upon
T3133 8211-8222 NN denotes stimulation
T3134 8223-8227 IN denotes with
T3135 8228-8232 JJ denotes live
T3136 8233-8237 NN denotes PAO1
T3137 8238-8240 CC denotes or
T3138 8241-8248 NN denotes LPS+ATP
T3139 8249-8251 IN denotes at
T3140 8252-8255 DT denotes the
T3141 8256-8261 NN denotes times
T3142 8262-8270 VB denotes examined
T3143 8271-8272 -LRB- denotes (
T3144 8272-8276 NNP denotes Fig.
T3145 8277-8281 NN denotes 3a–b
T3146 8281-8282 -RRB- denotes )
T3147 8284-8291 IN denotes Because
T3148 8292-8300 JJ denotes previous
T3149 8301-8308 NN denotes studies
T3150 8309-8313 VB denotes have
T3151 8314-8323 VB denotes indicated
T3152 8324-8325 DT denotes a
T3153 8326-8330 NN denotes role
T3154 8331-8334 IN denotes for
T3155 8335-8344 NN denotes caspase-1
T3156 8345-8347 IN denotes in
T3157 8348-8351 DT denotes the
T3158 8352-8362 NN denotes activation
T3159 8363-8365 IN denotes of
T3160 8366-8371 NN denotes NF-κB
T3161 8372-8379 IN denotes through
T3162 8380-8389 JJ denotes Toll-like
T3163 8390-8398 NN denotes receptor
T3164 8399-8400 -LRB- denotes (
T3165 8400-8403 NN denotes TLR
T3166 8403-8404 -RRB- denotes )
T3167 8405-8414 NN denotes signaling
T3168 8415-8416 -LRB- denotes [
T3169 8416-8418 CD denotes 35
T3170 8418-8419 -RRB- denotes ]
T3171 8419-8420 -COMMA- denotes ,
T3172 8421-8423 PRP denotes we
T3173 8424-8432 VB denotes examined
T3174 8433-8440 IN denotes whether
T3175 8441-8449 JJ denotes chemical
T3176 8450-8460 NN denotes inhibition
T3177 8461-8463 IN denotes of
T3178 8464-8473 NN denotes caspase-1
T3179 8474-8481 VB denotes altered
T3180 8482-8497 JJ denotes NF-κB-dependent
T3181 8498-8502 NN denotes IL-6
T3182 8503-8513 NN denotes production
T3183 8514-8516 IN denotes in
T3184 8517-8525 NN denotes response
T3185 8526-8528 TO denotes to
T3186 8529-8531 NNP denotes P.
T3187 8532-8542 NN denotes aeruginosa
T3188 8544-8551 RB denotes However
T3189 8551-8552 -COMMA- denotes ,
T3190 8553-8562 NN denotes treatment
T3191 8563-8567 IN denotes with
T3192 8568-8571 DT denotes the
T3193 8572-8581 NN denotes caspase-1
T3194 8582-8591 NN denotes inhibitor
T3195 8592-8602 NN denotes z-YVAD-fmk
T3196 8603-8604 -LRB- denotes (
T3197 8604-8608 NN denotes YVAD
T3198 8608-8609 -RRB- denotes )
T3199 8610-8613 VB denotes did
T3200 8614-8617 RB denotes not
T3201 8618-8626 VB denotes decrease
T3202 8627-8631 NN denotes IL-6
T3203 8632-8641 NN denotes secretion
T3204 8642-8644 IN denotes by
T3205 8645-8651 NN denotes airway
T3206 8652-8662 JJ denotes epithelial
T3207 8663-8668 NN denotes cells
T3208 8669-8670 -LRB- denotes (
T3209 8670-8674 NN denotes Fig.
T3210 8675-8677 NN denotes 3c
T3211 8677-8678 -RRB- denotes )
T3404 9425-9429 NN denotes CD14
T3405 9430-9438 JJ denotes positive
T3406 9439-9448 NN denotes monocytes
T3407 9449-9453 IN denotes from
T3408 9454-9456 NN denotes CF
T3409 9457-9465 NN denotes patients
T3410 9466-9469 CC denotes and
T3411 9470-9478 NN denotes controls
T3412 9479-9483 VB denotes show
T3413 9484-9491 JJ denotes similar
T3414 9492-9501 NN denotes increases
T3415 9502-9504 IN denotes in
T3416 9505-9514 NN denotes caspase-1
T3417 9515-9523 NN denotes activity
T3418 9524-9528 IN denotes upon
T3419 9529-9541 JJ denotes inflammasome
T3420 9542-9553 NN denotes stimulation
T3421 9554-9563 NN denotes Monocytes
T3422 9564-9568 VB denotes were
T3423 9569-9579 VB denotes identified
T3424 9580-9582 IN denotes in
T3425 9583-9587 NN denotes PBMC
T3426 9588-9599 NN denotes populations
T3427 9600-9605 VB denotes using
T3428 9606-9610 NN denotes CD14
T3429 9611-9613 IN denotes as
T3430 9614-9615 DT denotes a
T3431 9616-9627 NN denotes phenotyping
T3432 9628-9634 NN denotes marker
T3433 9636-9640 NN denotes CD14
T3434 9641-9649 JJ denotes positive
T3435 9650-9659 NN denotes monocytes
T3436 9660-9664 IN denotes from
T3437 9665-9667 NN denotes CF
T3438 9668-9676 NN denotes patients
T3439 9677-9680 CC denotes and
T3440 9681-9688 JJ denotes healthy
T3441 9689-9697 NN denotes controls
T3442 9698-9704 VB denotes showed
T3443 9705-9706 DT denotes a
T3444 9707-9718 JJ denotes significant
T3445 9719-9727 NN denotes increase
T3446 9728-9730 IN denotes in
T3447 9731-9740 NN denotes caspase-1
T3448 9741-9751 NN denotes activation
T3449 9752-9756 IN denotes upon
T3450 9757-9768 NN denotes stimulation
T3451 9769-9773 IN denotes with
T3452 9774-9781 NN denotes LPS+ATP
T3453 9781-9782 -COMMA- denotes ,
T3454 9783-9787 NN denotes PAO1
T3455 9787-9788 -COMMA- denotes ,
T3456 9789-9792 CC denotes and
T3457 9793-9801 NN denotes LPS+Poly
T3458 9801-9802 -LRB- denotes (
T3459 9802-9804 NN denotes dA
T3460 9804-9805 -COLON- denotes :
T3461 9805-9807 NN denotes dT
T3462 9807-9808 -RRB- denotes )
T3463 9809-9810 -LRB- denotes (
T3464 9810-9814 NN denotes Fig.
T3465 9815-9817 NN denotes 4a
T3466 9817-9818 -RRB- denotes )
T3467 9819-9822 CC denotes but
T3468 9823-9827 DT denotes this
T3469 9828-9838 NN denotes activation
T3470 9839-9842 VB denotes was
T3471 9843-9846 RB denotes not
T3472 9847-9856 JJ denotes different
T3473 9857-9864 IN denotes between
T3474 9865-9867 NN denotes CF
T3475 9868-9871 CC denotes and
T3476 9872-9879 NN denotes control
T3477 9880-9888 NN denotes subjects
T3478 9889-9890 -LRB- denotes (
T3479 9890-9894 NNP denotes Fig.
T3480 9895-9897 NN denotes 4b
T3481 9897-9898 -RRB- denotes )
T3638 10426-10431 NN denotes PBMCs
T3639 10432-10436 IN denotes from
T3640 10437-10439 NN denotes CF
T3641 10440-10448 NN denotes patients
T3642 10449-10451 VB denotes do
T3643 10452-10455 RB denotes not
T3644 10456-10463 VB denotes produce
T3645 10464-10473 VB denotes increased
T3646 10474-10481 NN denotes amounts
T3647 10482-10484 IN denotes of
T3648 10485-10490 NN denotes IL-1β
T3649 10491-10495 IN denotes upon
T3650 10496-10508 JJ denotes inflammasome
T3651 10509-10520 NN denotes stimulation
T3652 10521-10529 JJ denotes Previous
T3653 10530-10537 NN denotes studies
T3654 10538-10542 VB denotes have
T3655 10543-10548 VB denotes shown
T3656 10549-10553 IN denotes that
T3657 10554-10557 DT denotes the
T3658 10558-10562 NN denotes loss
T3659 10563-10565 IN denotes of
T3660 10566-10570 NN denotes CFTR
T3661 10571-10578 VB denotes results
T3662 10579-10581 IN denotes in
T3663 10582-10591 VB denotes increased
T3664 10592-10597 NN denotes NF-κB
T3665 10598-10606 NN denotes activity
T3666 10607-10610 CC denotes and
T3667 10611-10627 JJ denotes pro-inflammatory
T3668 10628-10636 NN denotes cytokine
T3669 10637-10646 NN denotes secretion
T3670 10647-10648 -LRB- denotes [
T3671 10648-10649 CD denotes 4
T3672 10649-10650 -RRB- denotes ]
T3673 10650-10651 -COMMA- denotes ,
T3674 10652-10653 -LRB- denotes [
T3675 10653-10654 CD denotes 5
T3676 10654-10655 -RRB- denotes ]
T3677 10655-10656 -COMMA- denotes ,
T3678 10657-10658 -LRB- denotes [
T3679 10658-10660 CD denotes 32
T3680 10660-10661 -RRB- denotes ]
T3681 10661-10662 -COMMA- denotes ,
T3682 10663-10664 -LRB- denotes [
T3683 10664-10666 CD denotes 36
T3684 10666-10667 -RRB- denotes ]
T3685 10667-10668 -COMMA- denotes ,
T3686 10669-10670 -LRB- denotes [
T3687 10670-10672 CD denotes 37
T3688 10672-10673 -RRB- denotes ]
T3689 10675-10677 TO denotes To
T3690 10678-10685 RB denotes further
T3691 10686-10693 VB denotes examine
T3692 10694-10698 DT denotes this
T3693 10699-10711 NN denotes relationship
T3694 10711-10712 -COMMA- denotes ,
T3695 10713-10718 NN denotes PBMCs
T3696 10719-10723 IN denotes from
T3697 10724-10726 NN denotes CF
T3698 10727-10735 NN denotes patients
T3699 10736-10739 CC denotes and
T3700 10740-10747 JJ denotes healthy
T3701 10748-10753 JJ denotes adult
T3702 10754-10762 NN denotes controls
T3703 10763-10767 VB denotes were
T3704 10768-10778 VB denotes stimulated
T3705 10779-10783 IN denotes with
T3706 10784-10788 NN denotes PAO1
T3707 10788-10789 -COMMA- denotes ,
T3708 10790-10797 NN denotes LPS+ATP
T3709 10797-10798 -COMMA- denotes ,
T3710 10799-10802 CC denotes and
T3711 10803-10811 NN denotes LPS+Poly
T3712 10811-10812 -LRB- denotes (
T3713 10812-10814 NN denotes dA
T3714 10814-10815 -COLON- denotes :
T3715 10815-10817 NN denotes dT
T3716 10817-10818 -RRB- denotes )
T3717 10818-10819 -COMMA- denotes ,
T3718 10820-10822 TO denotes to
T3719 10823-10831 VB denotes activate
T3720 10832-10835 DT denotes the
T3721 10836-10841 NN denotes NLRC4
T3722 10841-10842 -COMMA- denotes ,
T3723 10843-10848 NN denotes NLRP3
T3724 10848-10849 -COMMA- denotes ,
T3725 10850-10853 CC denotes and
T3726 10854-10858 NN denotes AIM2
T3727 10859-10872 NN denotes inflammasomes
T3728 10872-10873 -COMMA- denotes ,
T3729 10874-10886 RB denotes respectively
T3730 10888-10890 IN denotes By
T3731 10891-10893 CD denotes 24
T3732 10894-10899 NN denotes hours
T3733 10900-10902 IN denotes of
T3734 10903-10914 NN denotes stimulation
T3735 10914-10915 -COMMA- denotes ,
T3736 10916-10918 NN denotes CF
T3737 10919-10924 NN denotes PBMCs
T3738 10925-10928 VB denotes did
T3739 10929-10932 RB denotes not
T3740 10933-10940 VB denotes produce
T3741 10941-10950 VB denotes increased
T3742 10951-10958 NN denotes amounts
T3743 10959-10961 IN denotes of
T3744 10962-10967 NN denotes IL-1β
T3745 10968-10969 -LRB- denotes (
T3746 10969-10973 NN denotes Fig.
T3747 10974-10976 NN denotes 5a
T3748 10976-10977 -RRB- denotes )
T3749 10978-10980 CC denotes or
T3750 10981-10985 NN denotes IL-8
T3751 10986-10987 -LRB- denotes (
T3752 10987-10991 NN denotes Fig.
T3753 10992-10994 NN denotes 5b
T3754 10994-10995 -RRB- denotes )
T3755 10996-11000 WRB denotes when
T3756 11001-11009 VB denotes compared
T3757 11010-11012 TO denotes to
T3758 11013-11020 JJ denotes healthy
T3759 11021-11029 NN denotes controls
T3760 11031-11038 RB denotes However
T3761 11038-11039 -COMMA- denotes ,
T3762 11040-11042 PRP denotes we
T3763 11043-11046 VB denotes did
T3764 11047-11053 VB denotes notice
T3765 11054-11055 DT denotes a
T3766 11056-11065 JJ denotes transient
T3767 11066-11074 NN denotes decrease
T3768 11075-11076 -LRB- denotes (
T3769 11076-11077 NN denotes P
T3770 11077-11078 SYM denotes <
T3771 11078-11083 CD denotes 0.001
T3772 11083-11084 -RRB- denotes )
T3773 11085-11087 IN denotes in
T3774 11088-11091 DT denotes the
T3775 11092-11098 NN denotes amount
T3776 11099-11101 IN denotes of
T3777 11102-11107 NN denotes IL-1β
T3778 11108-11116 VB denotes produced
T3779 11117-11119 IN denotes by
T3780 11120-11122 NN denotes CF
T3781 11123-11128 NN denotes cells
T3782 11129-11131 IN denotes in
T3783 11132-11140 NN denotes response
T3784 11141-11143 TO denotes to
T3785 11144-11151 NN denotes LPS+ATP
T3786 11152-11154 IN denotes at
T3787 11155-11156 CD denotes 6
T3788 11157-11162 NN denotes hours
T3789 11163-11164 -LRB- denotes (
T3790 11164-11168 NN denotes data
T3791 11169-11172 RB denotes not
T3792 11173-11178 VB denotes shown
T3793 11178-11179 -RRB- denotes )
T3794 11181-11192 NN denotes Stimulation
T3795 11193-11195 IN denotes of
T3796 11196-11201 NN denotes PBMCs
T3797 11202-11206 IN denotes with
T3798 11207-11209 NNP denotes P.
T3799 11210-11220 NN denotes aeruginosa
T3800 11221-11225 WDT denotes that
T3801 11226-11231 VB denotes lacks
T3802 11232-11236 NN denotes exsA
T3803 11237-11238 -LRB- denotes (
T3804 11238-11247 NN denotes PAO1ΔexsA
T3805 11247-11248 -RRB- denotes )
T3806 11248-11249 -COMMA- denotes ,
T3807 11250-11251 DT denotes a
T3808 11252-11255 JJ denotes key
T3809 11256-11265 NN denotes regulator
T3810 11266-11268 IN denotes of
T3811 11269-11273 NN denotes type
T3812 11274-11277 CD denotes III
T3813 11278-11287 NN denotes secretion
T3814 11287-11288 -COMMA- denotes ,
T3815 11289-11297 VB denotes produced
T3816 11298-11308 RB denotes three-fold
T3817 11309-11313 JJ denotes less
T3818 11314-11319 NN denotes IL-1β
T3819 11320-11328 VB denotes compared
T3820 11329-11331 TO denotes to
T3821 11332-11335 DT denotes the
T3822 11336-11344 JJ denotes parental
T3823 11345-11349 NN denotes PAO1
T3824 11350-11356 NN denotes strain
T3825 11357-11359 IN denotes by
T3826 11360-11362 CD denotes 24
T3827 11363-11368 NN denotes hours
T3828 11369-11370 -LRB- denotes (
T3829 11370-11374 NNP denotes Fig.
T3830 11375-11377 NN denotes 5a
T3831 11377-11378 -RRB- denotes )
T3832 11380-11392 JJ denotes Inflammasome
T3833 11393-11404 NN denotes stimulation
T3834 11405-11412 IN denotes without
T3835 11413-11420 NN denotes priming
T3836 11421-11424 VB denotes did
T3837 11425-11428 RB denotes not
T3838 11429-11435 VB denotes result
T3839 11436-11438 IN denotes in
T3840 11439-11442 DT denotes any
T3841 11443-11448 NN denotes IL-1β
T3842 11449-11459 NN denotes production
T3843 11460-11462 IN denotes in
T3844 11463-11469 CC denotes either
T3845 11470-11472 NN denotes CF
T3846 11473-11475 CC denotes or
T3847 11476-11483 NN denotes control
T3848 11484-11489 NN denotes PBMCs
T3849 11491-11499 JJ denotes Contrary
T3850 11500-11502 TO denotes to
T3851 11503-11506 PRP-DOLLAR- denotes our
T3852 11507-11517 NN denotes hypothesis
T3853 11517-11518 -COMMA- denotes ,
T3854 11519-11524 DT denotes these
T3855 11525-11532 NN denotes results
T3856 11533-11541 VB denotes indicate
T3857 11542-11546 IN denotes that
T3858 11547-11552 NN denotes PBMCs
T3859 11553-11557 IN denotes from
T3860 11558-11560 NN denotes CF
T3861 11561-11569 NN denotes patients
T3862 11570-11572 VB denotes do
T3863 11573-11576 RB denotes not
T3864 11577-11584 VB denotes display
T3865 11585-11594 VB denotes increased
T3866 11595-11605 NN denotes production
T3867 11606-11608 IN denotes of
T3868 11609-11614 NN denotes IL-1β
T3869 11615-11617 CC denotes or
T3870 11618-11622 NN denotes IL-8
T3871 11623-11627 IN denotes with
T3872 11628-11640 JJ denotes inflammasome
T3873 11641-11651 NN denotes activation
T3874 11652-11655 CC denotes nor
T3875 11656-11658 VB denotes do
T3876 11659-11663 PRP denotes they
T3877 11664-11671 VB denotes suggest
T3878 11672-11675 DT denotes any
T3879 11676-11685 VB denotes increased
T3880 11686-11691 JJ denotes basal
T3881 11692-11694 CC denotes or
T3882 11695-11702 VB denotes induced
T3883 11703-11708 NN denotes NF-κB
T3884 11709-11717 NN denotes activity
T3885 11719-11724 DT denotes These
T3886 11725-11732 NN denotes results
T3887 11733-11736 VB denotes are
T3888 11737-11747 JJ denotes consistent
T3889 11748-11752 IN denotes with
T3890 11753-11756 PRP-DOLLAR- denotes our
T3891 11757-11768 NN denotes observation
T3892 11769-11773 IN denotes that
T3893 11774-11783 NN denotes caspase-1
T3894 11784-11792 NN denotes activity
T3895 11793-11795 VB denotes is
T3896 11796-11799 RB denotes not
T3897 11800-11809 JJ denotes different
T3898 11810-11817 IN denotes between
T3899 11818-11820 NN denotes CF
T3900 11821-11824 CC denotes and
T3901 11825-11832 NN denotes control
T3902 11833-11838 NN denotes PBMCs
T3903 11839-11840 -LRB- denotes (
T3904 11840-11844 NN denotes Fig.
T3905 11845-11846 CD denotes 4
T3906 11846-11847 -RRB- denotes )
T4392 12380-12385 NN denotes NF-κB
T4393 12386-12396 NN denotes activation
T4394 12397-12399 VB denotes is
T4395 12400-12408 VB denotes required
T4396 12409-12412 IN denotes for
T4397 12413-12418 NN denotes IL-1β
T4398 12419-12422 CC denotes and
T4399 12423-12427 NN denotes IL-8
T4400 12428-12437 NN denotes responses
T4401 12438-12440 TO denotes to
T4402 12441-12443 NNP denotes P.
T4403 12444-12454 NN denotes aeruginosa
T4404 12456-12458 PRP denotes We
T4405 12459-12468 VB denotes confirmed
T4406 12469-12472 DT denotes the
T4407 12473-12483 NN denotes dependence
T4408 12484-12486 IN denotes of
T4409 12487-12499 JJ denotes PAO1-induced
T4410 12500-12505 NN denotes IL-1β
T4411 12506-12509 CC denotes and
T4412 12510-12514 NN denotes IL-8
T4413 12515-12525 NN denotes production
T4414 12526-12528 IN denotes on
T4415 12529-12534 NN denotes NF-κB
T4416 12535-12545 NN denotes activation
T4417 12546-12551 VB denotes using
T4418 12552-12557 NN denotes THP-1
T4419 12558-12563 NN denotes cells
T4420 12564-12574 VB denotes expressing
T4421 12575-12576 DT denotes a
T4422 12577-12585 NN denotes reporter
T4423 12586-12592 VB denotes driven
T4424 12593-12595 IN denotes by
T4425 12596-12601 NN denotes NF-κB
T4426 12602-12605 CC denotes and
T4427 12606-12610 NN denotes AP-1
T4428 12611-12619 NN denotes response
T4429 12620-12628 NN denotes elements
T4430 12630-12632 PRP denotes We
T4431 12633-12638 VB denotes found
T4432 12639-12643 IN denotes that
T4433 12644-12655 NN denotes stimulation
T4434 12656-12658 IN denotes of
T4435 12659-12665 VB denotes primed
T4436 12666-12671 NN denotes THP-1
T4437 12672-12680 NN denotes reporter
T4438 12681-12686 NN denotes cells
T4439 12687-12691 IN denotes with
T4440 12692-12703 JJ denotes heat-killed
T4441 12704-12708 NN denotes PAO1
T4442 12709-12717 VB denotes produced
T4443 12718-12721 DT denotes the
T4444 12722-12729 JJ denotes highest
T4445 12730-12736 NN denotes levels
T4446 12737-12739 IN denotes of
T4447 12740-12750 NN denotes NF-κB/AP-1
T4448 12751-12761 NN denotes activation
T4449 12762-12763 -LRB- denotes (
T4450 12763-12767 NN denotes Fig.
T4451 12768-12770 NN denotes 6a
T4452 12770-12771 -RRB- denotes )
T4453 12772-12775 CC denotes and
T4454 12776-12780 DT denotes this
T4455 12781-12791 VB denotes correlated
T4456 12792-12796 IN denotes with
T4457 12797-12801 NN denotes IL-8
T4458 12802-12811 NN denotes secretion
T4459 12812-12813 -LRB- denotes (
T4460 12813-12817 NN denotes Fig.
T4461 12818-12820 NN denotes 6b
T4462 12820-12821 -RRB- denotes )
T4463 12822-12825 CC denotes but
T4464 12826-12836 JJ denotes negligible
T4465 12837-12844 NN denotes amounts
T4466 12845-12847 IN denotes of
T4467 12848-12853 NN denotes IL-1β
T4468 12854-12858 VB denotes were
T4469 12859-12867 VB denotes secreted
T4470 12868-12869 -LRB- denotes (
T4471 12869-12873 NNP denotes Fig.
T4472 12874-12876 NN denotes 6c
T4473 12876-12877 -RRB- denotes )
T4474 12879-12890 NN denotes Stimulation
T4475 12891-12893 IN denotes of
T4476 12894-12900 VB denotes primed
T4477 12901-12906 NN denotes THP-1
T4478 12907-12915 NN denotes reporter
T4479 12916-12921 NN denotes cells
T4480 12922-12926 IN denotes with
T4481 12927-12931 JJ denotes live
T4482 12932-12936 NN denotes PAO1
T4483 12937-12940 VB denotes did
T4484 12941-12944 RB denotes not
T4485 12945-12958 RB denotes significantly
T4486 12959-12967 VB denotes increase
T4487 12968-12978 NN denotes NF-κB/AP-1
T4488 12979-12987 NN denotes activity
T4489 12988-12989 -LRB- denotes (
T4490 12989-12993 NN denotes Fig.
T4491 12994-12996 NN denotes 6a
T4492 12996-12997 -RRB- denotes )
T4493 12998-13000 CC denotes or
T4494 13001-13005 NN denotes IL-8
T4495 13006-13015 NN denotes secretion
T4496 13016-13017 -LRB- denotes (
T4497 13017-13021 NN denotes Fig.
T4498 13022-13024 NN denotes 6b
T4499 13024-13025 -RRB- denotes )
T4500 13026-13030 IN denotes over
T4501 13031-13038 NN denotes priming
T4502 13039-13044 RB denotes alone
T4503 13046-13053 RB denotes However
T4504 13053-13054 -COMMA- denotes ,
T4505 13055-13060 NN denotes IL-1β
T4506 13061-13071 NN denotes production
T4507 13072-13075 VB denotes was
T4508 13076-13083 RB denotes greatly
T4509 13084-13093 VB denotes augmented
T4510 13094-13098 IN denotes over
T4511 13099-13105 VB denotes primed
T4512 13106-13111 NN denotes cells
T4513 13112-13122 VB denotes stimulated
T4514 13123-13127 IN denotes with
T4515 13128-13139 JJ denotes heat-killed
T4516 13140-13144 NN denotes PAO1
T4517 13145-13147 CC denotes or
T4518 13148-13156 JJ denotes unprimed
T4519 13157-13162 NN denotes cells
T4520 13163-13173 VB denotes stimulated
T4521 13174-13178 IN denotes with
T4522 13179-13183 JJ denotes live
T4523 13184-13188 NN denotes PAO1
T4524 13189-13190 -LRB- denotes (
T4525 13190-13194 NN denotes Fig.
T4526 13195-13197 NN denotes 6c
T4527 13197-13198 -RRB- denotes )
T4528 13200-13204 DT denotes This
T4529 13205-13214 VB denotes confirmed
T4530 13215-13219 IN denotes that
T4531 13220-13225 NN denotes NF-κB
T4532 13226-13236 NN denotes activation
T4533 13237-13242 RB denotes alone
T4534 13243-13245 VB denotes is
T4535 13246-13249 RB denotes not
T4536 13250-13260 JJ denotes sufficient
T4537 13261-13264 IN denotes for
T4538 13265-13272 JJ denotes maximal
T4539 13273-13278 NN denotes IL-1β
T4540 13279-13288 NN denotes secretion
T4541 13288-13289 -COMMA- denotes ,
T4542 13290-13293 CC denotes but
T4543 13294-13303 VB denotes increased
T4544 13304-13311 NN denotes priming
T4545 13312-13314 IN denotes of
T4546 13315-13320 NN denotes NF-κB
T4547 13321-13323 VB denotes is
T4548 13324-13331 JJ denotes capable
T4549 13332-13334 IN denotes of
T4550 13335-13345 VB denotes augmenting
T4551 13346-13351 NN denotes IL-1β
T4552 13352-13362 NN denotes production
T4553 13363-13366 CC denotes and
T4554 13367-13376 NN denotes secretion
T4555 13377-13381 IN denotes upon
T4556 13382-13394 JJ denotes inflammasome
T4557 13395-13406 NN denotes stimulation
T4558 13408-13418 NN denotes Dependency
T4559 13419-13421 IN denotes of
T4560 13422-13427 DT denotes these
T4561 13428-13437 NN denotes responses
T4562 13438-13440 IN denotes on
T4563 13441-13446 NN denotes NF-κB
T4564 13447-13450 VB denotes was
T4565 13451-13460 VB denotes confirmed
T4566 13461-13463 IN denotes by
T4567 13464-13477 JJ denotes pharmacologic
T4568 13478-13488 NN denotes inhibition
T4569 13489-13491 IN denotes of
T4570 13492-13497 NN denotes NF-κB
T4571 13498-13503 VB denotes using
T4572 13504-13507 DT denotes the
T4573 13508-13518 NN denotes Bay11-7082
T4574 13519-13528 NN denotes inhibitor
T4575 13529-13531 IN denotes of
T4576 13532-13536 NN denotes IκBα
T4577 13537-13552 NN denotes phosphorylation
T4578 13552-13553 -COMMA- denotes ,
T4579 13554-13559 WDT denotes which
T4580 13560-13573 RB denotes significantly
T4581 13574-13581 VB denotes reduced
T4582 13582-13592 NN denotes NF-κB/AP-1
T4583 13593-13603 NN denotes activation
T4584 13604-13605 -LRB- denotes (
T4585 13605-13606 NN denotes P
T4586 13606-13607 SYM denotes <
T4587 13607-13612 CD denotes 0.001
T4588 13612-13613 -RRB- denotes )
T4589 13614-13615 -LRB- denotes (
T4590 13615-13619 NNP denotes Fig.
T4591 13620-13622 NNP denotes 6d
T4592 13622-13623 -RRB- denotes )
T4593 13624-13627 CC denotes and
T4594 13628-13631 DT denotes the
T4595 13632-13642 JJ denotes subsequent
T4596 13643-13653 NN denotes production
T4597 13654-13656 IN denotes of
T4598 13657-13661 NN denotes IL-8
T4599 13662-13663 -LRB- denotes (
T4600 13663-13664 NN denotes P
T4601 13664-13665 SYM denotes <
T4602 13665-13669 CD denotes 0.01
T4603 13669-13670 -RRB- denotes )
T4604 13671-13672 -LRB- denotes (
T4605 13672-13676 NNP denotes Fig.
T4606 13677-13679 NNP denotes 6e
T4607 13679-13680 -RRB- denotes )
T4608 13681-13684 CC denotes and
T4609 13685-13690 NN denotes IL-1β
T4610 13691-13692 -LRB- denotes (
T4611 13692-13693 NN denotes P
T4612 13693-13694 SYM denotes <
T4613 13694-13699 CD denotes 0.001
T4614 13699-13700 -RRB- denotes )
T4615 13701-13702 -LRB- denotes (
T4616 13702-13706 NNP denotes Fig.
T4617 13707-13709 CD denotes 6f
T4618 13709-13710 -RRB- denotes )
T4619 13711-13713 IN denotes in
T4620 13714-13722 NN denotes response
T4621 13723-13725 TO denotes to
T4622 13726-13730 CC denotes both
T4623 13731-13742 JJ denotes heat-killed
T4624 13743-13746 CC denotes and
T4625 13747-13751 JJ denotes live
T4626 13752-13756 NN denotes PAO1
T4627 13758-13763 DT denotes These
T4628 13764-13771 NN denotes results
T4629 13772-13776 VB denotes were
T4630 13777-13781 RB denotes also
T4631 13782-13790 VB denotes verified
T4632 13791-13793 IN denotes in
T4633 13794-13796 NN denotes CF
T4634 13797-13800 CC denotes and
T4635 13801-13808 NN denotes control
T4636 13809-13814 NN denotes PBMCs
T4637 13815-13818 IN denotes for
T4638 13819-13823 DT denotes each
T4639 13824-13836 NN denotes inflammasome
T4640 13837-13845 VB denotes examined
T4641 13846-13847 -LRB- denotes (
T4642 13847-13848 NN denotes P
T4643 13848-13849 SYM denotes <
T4644 13849-13854 CD denotes 0.001
T4645 13854-13855 -RRB- denotes )
T4646 13856-13857 -LRB- denotes (
T4647 13857-13861 NNP denotes Fig.
T4648 13862-13866 NNP denotes 6g–h
T4649 13866-13867 -RRB- denotes )
T4650 13869-13876 RB denotes Overall
T4651 13877-13882 DT denotes these
T4652 13883-13890 NN denotes results
T4653 13891-13898 VB denotes confirm
T4654 13899-13903 IN denotes that
T4655 13904-13909 NN denotes NF-κB
T4656 13910-13912 VB denotes is
T4657 13913-13915 DT denotes an
T4658 13916-13925 JJ denotes important
T4659 13926-13935 NN denotes modulator
T4660 13936-13938 IN denotes of
T4661 13939-13944 NN denotes IL-1β
T4662 13945-13955 NN denotes production
T4663 13956-13959 CC denotes and
T4664 13960-13964 IN denotes that
T4665 13965-13974 VB denotes increased
T4666 13975-13985 NN denotes activation
T4667 13986-13988 IN denotes of
T4668 13989-13994 NN denotes NF-κB
T4669 13995-14003 VB denotes augments
T4670 14004-14025 JJ denotes inflammasome-mediated
T4671 14026-14036 NN denotes production
T4672 14037-14039 IN denotes of
T4673 14040-14045 NN denotes IL-1β
T5212 15230-15240 NN denotes Bay11-7082
T5213 15241-15249 VB denotes inhibits
T5214 15250-15259 JJ denotes pro-IL-1β
T5215 15260-15270 NN denotes production
T5216 15271-15273 IN denotes in
T5217 15274-15282 NN denotes response
T5218 15283-15285 TO denotes to
T5219 15286-15288 NNP denotes P.
T5220 15289-15299 NN denotes aeruginosa
T5221 15301-15303 IN denotes In
T5222 15304-15312 NN denotes addition
T5223 15313-15315 TO denotes to
T5224 15316-15326 NN denotes inhibition
T5225 15327-15329 IN denotes of
T5226 15330-15335 NN denotes NF-κB
T5227 15336-15344 NN denotes activity
T5228 15344-15345 -COMMA- denotes ,
T5229 15346-15356 NN denotes Bay11-7082
T5230 15357-15360 MD denotes can
T5231 15361-15365 RB denotes also
T5232 15366-15374 RB denotes directly
T5233 15375-15382 VB denotes inhibit
T5234 15383-15386 DT denotes the
T5235 15387-15392 NN denotes NLRP3
T5236 15393-15405 NN denotes inflammasome
T5237 15406-15407 -LRB- denotes [
T5238 15407-15409 CD denotes 38
T5239 15409-15410 -RRB- denotes ]
T5240 15412-15414 TO denotes To
T5241 15415-15423 VB denotes validate
T5242 15424-15427 PRP-DOLLAR- denotes its
T5243 15428-15431 NN denotes use
T5244 15432-15434 IN denotes in
T5245 15435-15439 DT denotes this
T5246 15440-15445 NN denotes study
T5247 15446-15448 IN denotes as
T5248 15449-15451 DT denotes an
T5249 15452-15457 NN denotes NF-κB
T5250 15458-15467 NN denotes inhibitor
T5251 15467-15468 -COMMA- denotes ,
T5252 15469-15476 JJ denotes western
T5253 15477-15482 NN denotes blots
T5254 15483-15486 IN denotes for
T5255 15487-15496 NN denotes pro-IL-1β
T5256 15497-15501 VB denotes were
T5257 15502-15511 VB denotes performed
T5258 15512-15521 IN denotes alongside
T5259 15522-15532 NN denotes inhibition
T5260 15533-15535 IN denotes of
T5261 15536-15540 NN denotes CFTR
T5262 15541-15549 NN denotes activity
T5263 15550-15552 IN denotes by
T5264 15553-15563 NN denotes CFTRinh172
T5265 15564-15566 IN denotes in
T5266 15567-15575 NN denotes response
T5267 15576-15578 TO denotes to
T5268 15579-15583 NN denotes PAO1
T5269 15584-15586 IN denotes at
T5270 15587-15588 CD denotes 4
T5271 15589-15594 NN denotes hours
T5272 15595-15600 IN denotes after
T5273 15601-15612 NN denotes stimulation
T5274 15613-15614 -LRB- denotes (
T5275 15614-15618 NN denotes Fig.
T5276 15619-15621 NN denotes 7a
T5277 15621-15622 -RRB- denotes )
T5278 15624-15627 PRP-DOLLAR- denotes Our
T5279 15628-15635 NN denotes results
T5280 15636-15644 VB denotes indicate
T5281 15645-15649 IN denotes that
T5282 15650-15660 NN denotes Bay11-7082
T5283 15661-15669 VB denotes prevents
T5284 15670-15680 NN denotes production
T5285 15681-15683 IN denotes of
T5286 15684-15693 NN denotes pro-IL-1β
T5287 15694-15701 IN denotes whereas
T5288 15702-15712 NN denotes CFTRinh172
T5289 15713-15717 VB denotes does
T5290 15718-15721 RB denotes not
T5291 15722-15726 VB denotes seem
T5292 15727-15729 TO denotes to
T5293 15730-15736 VB denotes affect
T5294 15737-15739 PRP denotes it
T5295 15741-15745 DT denotes This
T5296 15746-15749 VB denotes was
T5297 15750-15757 RB denotes further
T5298 15758-15770 VB denotes corroborated
T5299 15771-15773 IN denotes by
T5300 15774-15777 DT denotes the
T5301 15778-15785 NN denotes ability
T5302 15786-15796 NN denotes Bay11-7082
T5303 15797-15799 TO denotes to
T5304 15800-15807 VB denotes inhibit
T5305 15808-15812 NN denotes IκBα
T5306 15813-15824 NN denotes degradation
T5307 15825-15827 IN denotes at
T5308 15828-15831 CD denotes 0.5
T5309 15831-15832 -COMMA- denotes ,
T5310 15833-15834 CD denotes 1
T5311 15834-15835 -COMMA- denotes ,
T5312 15836-15839 CC denotes and
T5313 15840-15843 CD denotes 1.5
T5314 15844-15849 NN denotes hours
T5315 15850-15854 VB denotes post
T5316 15855-15859 NN denotes PAO1
T5317 15860-15871 NN denotes stimulation
T5318 15872-15873 -LRB- denotes (
T5319 15873-15877 NN denotes Fig.
T5320 15878-15880 NN denotes 7b
T5321 15880-15881 -RRB- denotes )
T5503 16297-16307 NN denotes Disruption
T5504 16308-16310 IN denotes of
T5505 16311-16315 NN denotes CFTR
T5506 16316-16324 NN denotes activity
T5507 16325-16329 VB denotes does
T5508 16330-16333 RB denotes not
T5509 16334-16342 VB denotes increase
T5510 16343-16348 NN denotes IL-1β
T5511 16349-16359 NN denotes production
T5512 16360-16362 IN denotes in
T5513 16363-16368 NN denotes PBMCs
T5514 16369-16372 CC denotes and
T5515 16373-16378 NN denotes THP-1
T5516 16379-16384 NN denotes cells
T5517 16385-16386 DT denotes A
T5518 16387-16395 JJ denotes previous
T5519 16396-16401 NN denotes study
T5520 16402-16405 VB denotes has
T5521 16406-16415 VB denotes indicated
T5522 16416-16417 DT denotes a
T5523 16418-16422 NN denotes role
T5524 16423-16426 IN denotes for
T5525 16427-16435 NN denotes chloride
T5526 16436-16439 NN denotes ion
T5527 16440-16453 NN denotes concentration
T5528 16454-16456 IN denotes in
T5529 16457-16468 NN denotes suppression
T5530 16469-16471 IN denotes of
T5531 16472-16477 NN denotes NLRP3
T5532 16478-16490 JJ denotes inflammasome
T5533 16491-16501 NN denotes activation
T5534 16502-16503 -LRB- denotes [
T5535 16503-16505 CD denotes 34
T5536 16505-16506 -RRB- denotes ]
T5537 16508-16510 TO denotes To
T5538 16511-16520 VB denotes determine
T5539 16521-16528 IN denotes whether
T5540 16529-16533 NN denotes CFTR
T5541 16534-16545 NN denotes dysfunction
T5542 16546-16552 VB denotes alters
T5543 16553-16558 NN denotes IL-1β
T5544 16559-16569 NN denotes production
T5545 16569-16570 -COMMA- denotes ,
T5546 16571-16576 NN denotes THP-1
T5547 16577-16582 NN denotes cells
T5548 16583-16586 CC denotes and
T5549 16587-16592 NN denotes PBMCs
T5550 16593-16597 IN denotes from
T5551 16598-16600 NN denotes CF
T5552 16601-16609 NN denotes patients
T5553 16610-16613 CC denotes and
T5554 16614-16621 JJ denotes healthy
T5555 16622-16630 NN denotes controls
T5556 16631-16635 VB denotes were
T5557 16636-16643 VB denotes treated
T5558 16644-16648 IN denotes with
T5559 16649-16652 DT denotes the
T5560 16653-16657 NN denotes CFTR
T5561 16658-16667 NN denotes inhibitor
T5562 16667-16668 -COMMA- denotes ,
T5563 16669-16679 NN denotes CFTRinh172
T5564 16679-16680 -COMMA- denotes ,
T5565 16681-16686 JJ denotes prior
T5566 16687-16689 TO denotes to
T5567 16690-16700 NN denotes simulation
T5568 16701-16705 IN denotes with
T5569 16706-16710 JJ denotes live
T5570 16711-16713 NNP denotes P.
T5571 16714-16724 NN denotes aeruginosa
T5572 16726-16735 NN denotes Treatment
T5573 16736-16740 IN denotes with
T5574 16741-16751 NN denotes CFTRinh172
T5575 16752-16755 VB denotes did
T5576 16756-16759 RB denotes not
T5577 16760-16765 VB denotes alter
T5578 16766-16771 NN denotes IL-1β
T5579 16772-16774 CC denotes or
T5580 16775-16779 NN denotes IL-8
T5581 16780-16790 NN denotes production
T5582 16791-16793 IN denotes in
T5583 16794-16801 JJ denotes control
T5584 16802-16810 NN denotes subjects
T5585 16811-16813 CC denotes or
T5586 16814-16816 NN denotes CF
T5587 16817-16825 NN denotes patients
T5588 16826-16827 -LRB- denotes (
T5589 16827-16831 NN denotes Fig.
T5590 16832-16836 NN denotes 8a–b
T5591 16836-16837 -RRB- denotes )
T5592 16839-16848 RB denotes Similarly
T5593 16848-16849 -COMMA- denotes ,
T5594 16850-16855 NN denotes IL-1β
T5595 16856-16866 NN denotes production
T5596 16867-16870 VB denotes was
T5597 16871-16874 RB denotes not
T5598 16875-16884 JJ denotes different
T5599 16885-16887 IN denotes in
T5600 16888-16904 JJ denotes monocyte-derived
T5601 16905-16916 NN denotes macrophages
T5602 16917-16919 CC denotes or
T5603 16920-16925 NN denotes THP-1
T5604 16926-16934 NN denotes reporter
T5605 16935-16940 NN denotes cells
T5606 16941-16948 VB denotes treated
T5607 16949-16953 IN denotes with
T5608 16954-16964 NN denotes CFTRinh172
T5609 16965-16966 -LRB- denotes (
T5610 16966-16970 NNP denotes Fig.
T5611 16971-16975 NNP denotes 8c–d
T5612 16975-16976 -RRB- denotes )
T5613 16978-16982 NN denotes IL-8
T5614 16983-16984 -LRB- denotes (
T5615 16984-16988 NN denotes Fig.
T5616 16989-16991 NN denotes 8e
T5617 16991-16992 -RRB- denotes )
T5618 16993-16996 CC denotes and
T5619 16997-17002 NN denotes NF-κB
T5620 17003-17011 NN denotes activity
T5621 17012-17013 -LRB- denotes (
T5622 17013-17017 NNP denotes Fig.
T5623 17018-17020 NN denotes 8f
T5624 17020-17021 -RRB- denotes )
T5625 17022-17026 VB denotes were
T5626 17027-17031 RB denotes also
T5627 17032-17041 JJ denotes unchanged
T5628 17042-17044 IN denotes in
T5629 17045-17063 JJ denotes CFTRinh172-treated
T5630 17064-17069 NN denotes THP-1
T5631 17070-17078 NN denotes reporter
T5632 17079-17084 NN denotes cells
T6078 17820-17826 NN denotes Levels
T6079 17827-17829 IN denotes of
T6080 17830-17835 NN denotes IL-1β
T6081 17836-17839 VB denotes are
T6082 17840-17849 VB denotes increased
T6083 17850-17852 IN denotes in
T6084 17853-17856 DT denotes the
T6085 17857-17861 NN denotes BALF
T6086 17862-17864 IN denotes of
T6087 17865-17867 NN denotes CF
T6088 17868-17876 NN denotes patients
T6089 17877-17880 CC denotes but
T6090 17881-17884 DT denotes the
T6091 17885-17893 JJ denotes cellular
T6092 17894-17900 NN denotes source
T6093 17901-17903 IN denotes of
T6094 17904-17908 DT denotes this
T6095 17909-17917 NN denotes cytokine
T6096 17918-17921 CC denotes and
T6097 17922-17925 PRP-DOLLAR- denotes its
T6098 17926-17936 NN denotes production
T6099 17937-17939 IN denotes in
T6100 17940-17943 DT denotes the
T6101 17944-17951 NN denotes context
T6102 17952-17954 IN denotes of
T6103 17955-17963 VB denotes targeted
T6104 17964-17976 JJ denotes inflammasome
T6105 17977-17987 NN denotes activation
T6106 17988-17991 VB denotes are
T6107 17992-17997 RB denotes still
T6108 17998-18005 JJ denotes unclear
T6109 18007-18009 PRP denotes We
T6110 18010-18015 RB denotes first
T6111 18016-18023 VB denotes studied
T6112 18024-18030 NN denotes airway
T6113 18031-18041 JJ denotes epithelial
T6114 18042-18047 NN denotes cells
T6115 18048-18051 JJ denotes due
T6116 18052-18054 TO denotes to
T6117 18055-18060 PRP-DOLLAR- denotes their
T6118 18061-18065 NN denotes role
T6119 18066-18068 IN denotes in
T6120 18069-18076 NN denotes barrier
T6121 18077-18085 NN denotes function
T6122 18085-18086 -COMMA- denotes ,
T6123 18087-18096 NN denotes proximity
T6124 18097-18099 TO denotes to
T6125 18100-18109 NN denotes infection
T6126 18109-18110 -COMMA- denotes ,
T6127 18111-18114 CC denotes and
T6128 18115-18122 NN denotes ability
T6129 18123-18125 TO denotes to
T6130 18126-18133 VB denotes produce
T6131 18134-18138 JJ denotes high
T6132 18139-18145 NN denotes levels
T6133 18146-18148 IN denotes of
T6134 18149-18165 JJ denotes pro-inflammatory
T6135 18166-18175 NN denotes cytokines
T6136 18177-18184 RB denotes However
T6137 18184-18185 -COMMA- denotes ,
T6138 18186-18188 PRP denotes we
T6139 18189-18194 VB denotes found
T6140 18195-18199 IN denotes that
T6141 18200-18209 JJ denotes bronchial
T6142 18210-18220 JJ denotes epithelial
T6143 18221-18226 NN denotes cells
T6144 18227-18229 VB denotes do
T6145 18230-18233 RB denotes not
T6146 18234-18241 VB denotes produce
T6147 18242-18253 JJ denotes significant
T6148 18254-18261 NN denotes amounts
T6149 18262-18264 IN denotes of
T6150 18265-18270 NN denotes IL-1β
T6151 18271-18274 CC denotes and
T6152 18275-18277 VB denotes do
T6153 18278-18281 RB denotes not
T6154 18282-18286 VB denotes show
T6155 18287-18288 DT denotes a
T6156 18289-18300 JJ denotes significant
T6157 18301-18309 NN denotes increase
T6158 18310-18312 IN denotes in
T6159 18313-18322 NN denotes caspase-1
T6160 18323-18333 NN denotes activation
T6161 18334-18336 IN denotes in
T6162 18337-18345 NN denotes response
T6163 18346-18348 TO denotes to
T6164 18349-18353 NN denotes PAO1
T6165 18354-18357 CC denotes and
T6166 18358-18365 NN denotes LPS+ATP
T6167 18365-18366 -COMMA- denotes ,
T6168 18367-18369 IN denotes in
T6169 18370-18380 NN denotes comparison
T6170 18381-18383 TO denotes to
T6171 18384-18397 JJ denotes hematopoeitic
T6172 18398-18409 JJ denotes mononuclear
T6173 18410-18415 NN denotes cells
T6174 18417-18442 JJ denotes Hematopoeitically-derived
T6175 18443-18448 NN denotes cells
T6176 18448-18449 -COMMA- denotes ,
T6177 18450-18454 JJ denotes such
T6178 18455-18457 IN denotes as
T6179 18458-18467 NN denotes monocytes
T6180 18468-18471 CC denotes and
T6181 18472-18483 NN denotes macrophages
T6182 18483-18484 -COMMA- denotes ,
T6183 18485-18491 VB denotes appear
T6184 18492-18494 TO denotes to
T6185 18495-18497 VB denotes be
T6186 18498-18499 DT denotes a
T6187 18500-18509 JJ denotes principal
T6188 18510-18516 NN denotes source
T6189 18517-18519 IN denotes of
T6190 18520-18525 NN denotes IL-1β
T6191 18527-18531 NN denotes CFTR
T6192 18532-18534 VB denotes is
T6193 18535-18544 VB denotes expressed
T6194 18545-18547 IN denotes in
T6196 18557-18568 NN denotes macrophages
T6197 18569-18570 -LRB- denotes [
T6198 18570-18572 CD denotes 32
T6199 18572-18573 -RRB- denotes ]
T6200 18573-18574 -COMMA- denotes ,
T6201 18575-18576 -LRB- denotes [
T6202 18576-18578 CD denotes 39
T6203 18578-18579 -RRB- denotes ]
T6204 18580-18582 RB denotes as
T6205 18583-18587 RB denotes well
T6206 18588-18590 IN denotes as
T6207 18591-18593 IN denotes in
T6208 18594-18599 NN denotes PBMCs
T6209 18600-18602 IN denotes at
T6210 18603-18607 CC denotes both
T6211 18608-18611 DT denotes the
T6212 18612-18616 NN denotes mRNA
T6213 18617-18620 CC denotes and
T6214 18621-18628 NN denotes protein
T6215 18629-18634 NN denotes level
T6216 18635-18636 -LRB- denotes [
T6217 18636-18638 CD denotes 40
T6218 18638-18639 -RRB- denotes ]
T6219 18639-18640 -COMMA- denotes ,
T6220 18641-18642 -LRB- denotes [
T6221 18642-18644 CD denotes 41
T6222 18644-18645 -RRB- denotes ]
T6223 18645-18646 -COMMA- denotes ,
T6224 18647-18650 CC denotes and
T6225 18651-18654 PRP-DOLLAR- denotes its
T6226 18655-18659 NN denotes loss
T6227 18660-18662 VB denotes is
T6228 18663-18673 RB denotes frequently
T6229 18674-18684 VB denotes associated
T6230 18685-18689 IN denotes with
T6231 18690-18692 DT denotes an
T6232 18693-18702 JJ denotes augmented
T6233 18703-18715 JJ denotes inflammatory
T6234 18716-18725 NN denotes phenotype
T6235 18727-18734 IN denotes Despite
T6236 18735-18738 PRP-DOLLAR- denotes our
T6237 18739-18747 NN denotes findings
T6238 18748-18758 VB denotes indicating
T6239 18759-18763 IN denotes that
T6240 18764-18773 JJ denotes bronchial
T6241 18774-18784 JJ denotes epithelial
T6242 18785-18790 NN denotes cells
T6243 18791-18795 WRB denotes when
T6244 18796-18801 VB denotes grown
T6245 18802-18804 FW denotes in
T6246 18805-18810 FW denotes vitro
T6247 18811-18814 VB denotes are
T6248 18815-18823 JJ denotes unlikely
T6249 18824-18826 TO denotes to
T6250 18827-18829 VB denotes be
T6251 18830-18843 RB denotes significantly
T6252 18844-18852 VB denotes involved
T6253 18853-18855 IN denotes in
T6254 18856-18859 DT denotes the
T6255 18860-18866 JJ denotes direct
T6256 18867-18877 NN denotes production
T6257 18878-18880 IN denotes of
T6258 18881-18886 NN denotes IL-1β
T6259 18887-18888 -LRB- denotes (
T6260 18888-18892 NNP denotes Fig.
T6261 18893-18897 NN denotes 2a–d
T6262 18897-18898 -RRB- denotes )
T6263 18898-18899 -COMMA- denotes ,
T6264 18900-18906 NN denotes others
T6265 18907-18911 VB denotes have
T6266 18912-18917 VB denotes shown
T6267 18918-18923 PRP-DOLLAR- denotes their
T6268 18924-18932 NN denotes capacity
T6269 18933-18935 TO denotes to
T6270 18936-18943 VB denotes respond
T6271 18944-18946 TO denotes to
T6272 18947-18955 JJ denotes alveolar
T6273 18956-18974 JJ denotes macrophage-derived
T6274 18975-18980 NN denotes IL-1β
T6275 18981-18984 CC denotes and
T6276 18985-18987 TO denotes to
T6277 18988-18995 VB denotes amplify
T6278 18996-18999 DT denotes the
T6279 19000-19012 JJ denotes inflammatory
T6280 19013-19021 NN denotes response
T6281 19022-19029 IN denotes through
T6282 19030-19033 DT denotes the
T6283 19034-19043 NN denotes induction
T6284 19044-19046 IN denotes of
T6285 19047-19057 NN denotes chemokines
T6286 19058-19061 CC denotes and
T6287 19062-19073 NN denotes recruitment
T6288 19074-19076 IN denotes of
T6289 19077-19089 JJ denotes inflammatory
T6290 19090-19098 NN denotes effector
T6291 19099-19104 NN denotes cells
T6292 19105-19106 -LRB- denotes [
T6293 19106-19108 CD denotes 42
T6294 19108-19109 -RRB- denotes ]
T6295 19111-19115 DT denotes This
T6296 19116-19127 NN denotes interaction
T6297 19128-19131 MD denotes may
T6298 19132-19142 VB denotes constitute
T6299 19143-19144 DT denotes a
T6300 19145-19153 JJ denotes critical
T6301 19154-19163 NN denotes component
T6302 19164-19166 TO denotes to
T6303 19167-19176 JJ denotes effective
T6304 19177-19181 NN denotes host
T6305 19182-19189 NN denotes defense
T6306 19190-19193 CC denotes and
T6307 19194-19205 VB denotes diminishing
T6308 19206-19209 DT denotes the
T6309 19210-19218 NN denotes capacity
T6310 19219-19221 IN denotes of
T6311 19222-19226 NN denotes host
T6312 19227-19232 NN denotes cells
T6313 19233-19235 TO denotes to
T6314 19236-19243 VB denotes respond
T6315 19244-19246 TO denotes to
T6316 19247-19252 NN denotes IL-1β
T6317 19253-19256 MD denotes may
T6318 19257-19262 VB denotes leave
T6319 19263-19266 DT denotes the
T6320 19267-19271 NN denotes host
T6321 19272-19283 JJ denotes susceptible
T6322 19284-19286 TO denotes to
T6323 19287-19297 NN denotes infections
T6324 19298-19300 IN denotes by
T6325 19301-19310 NN denotes pathogens
T6326 19311-19315 JJ denotes such
T6327 19316-19318 IN denotes as
T6328 19319-19321 NNP denotes P.
T6329 19322-19332 NNP denotes aeruginosa
T6330 19333-19334 -LRB- denotes [
T6331 19334-19336 CD denotes 43
T6332 19336-19337 -RRB- denotes ]
T6333 19339-19349 RB denotes Conversely
T6334 19349-19350 -COMMA- denotes ,
T6335 19351-19365 NN denotes overproduction
T6336 19366-19368 IN denotes of
T6337 19369-19374 NN denotes IL-1β
T6338 19375-19378 MD denotes can
T6339 19379-19383 RB denotes also
T6340 19384-19388 VB denotes play
T6341 19389-19390 DT denotes a
T6342 19391-19394 JJ denotes key
T6343 19395-19399 NN denotes role
T6344 19400-19402 IN denotes in
T6345 19403-19410 JJ denotes chronic
T6346 19411-19423 JJ denotes inflammatory
T6347 19424-19433 NN denotes responses
T6348 19434-19437 CC denotes and
T6349 19438-19443 VB denotes cause
T6350 19444-19450 NN denotes damage
T6351 19451-19453 TO denotes to
T6352 19454-19457 DT denotes the
T6353 19458-19462 NN denotes lung
T6354 19463-19473 NN denotes parenchyma
T6355 19474-19475 -LRB- denotes [
T6356 19475-19477 CD denotes 44
T6357 19477-19478 -RRB- denotes ]
T6358 19478-19479 -COMMA- denotes ,
T6359 19480-19481 -LRB- denotes [
T6360 19481-19483 CD denotes 45
T6361 19483-19484 -RRB- denotes ]
T6362 19486-19494 IN denotes Although
T6363 19495-19497 PRP denotes we
T6364 19498-19510 VB denotes hypothesized
T6365 19511-19515 IN denotes that
T6366 19516-19518 NN denotes CF
T6367 19519-19524 NN denotes cells
T6368 19525-19530 MD denotes would
T6369 19531-19538 VB denotes secrete
T6370 19539-19548 VB denotes increased
T6371 19549-19556 NN denotes amounts
T6372 19557-19559 IN denotes of
T6373 19560-19565 NN denotes IL-1β
T6374 19565-19566 -COMMA- denotes ,
T6375 19567-19569 PRP denotes we
T6376 19570-19575 VB denotes found
T6377 19576-19580 IN denotes that
T6378 19581-19586 NN denotes IL-1β
T6379 19587-19597 NN denotes production
T6380 19598-19600 IN denotes in
T6381 19601-19603 NN denotes CF
T6382 19604-19609 NN denotes PBMCs
T6383 19610-19613 VB denotes was
T6384 19614-19617 RB denotes not
T6385 19618-19627 VB denotes increased
T6386 19628-19632 IN denotes upon
T6387 19633-19645 JJ denotes inflammasome
T6388 19646-19657 NN denotes stimulation
T6389 19658-19660 IN denotes as
T6390 19661-19669 VB denotes compared
T6391 19670-19672 TO denotes to
T6392 19673-19681 NN denotes controls
T6393 19682-19683 -LRB- denotes (
T6394 19683-19687 NNP denotes Fig.
T6395 19688-19692 NN denotes 5a–b
T6396 19692-19693 -RRB- denotes )
T6397 19695-19699 DT denotes This
T6398 19700-19703 VB denotes was
T6399 19704-19706 IN denotes in
T6400 19707-19715 NN denotes contrast
T6401 19716-19718 TO denotes to
T6402 19719-19720 DT denotes a
T6403 19721-19729 JJ denotes previous
T6404 19730-19735 NN denotes study
T6405 19736-19740 IN denotes from
T6406 19741-19744 PRP-DOLLAR- denotes our
T6407 19745-19750 NN denotes group
T6408 19750-19751 -COMMA- denotes ,
T6409 19752-19757 WDT denotes which
T6410 19758-19764 VB denotes showed
T6411 19765-19774 VB denotes increased
T6412 19775-19780 NN denotes IL-1β
T6413 19781-19791 NN denotes production
T6414 19792-19794 IN denotes by
T6415 19795-19797 NN denotes CF
T6416 19798-19803 NN denotes PBMCs
T6417 19804-19806 IN denotes in
T6418 19807-19815 NN denotes response
T6419 19816-19818 TO denotes to
T6420 19819-19822 NN denotes LPS
T6421 19823-19824 -LRB- denotes [
T6422 19824-19826 CD denotes 46
T6423 19826-19827 -RRB- denotes ]
T6424 19827-19828 -COMMA- denotes ,
T6425 19829-19837 IN denotes although
T6426 19838-19842 DT denotes this
T6427 19843-19853 NN denotes difference
T6428 19854-19857 MD denotes may
T6429 19858-19860 VB denotes be
T6430 19861-19870 VB denotes accounted
T6431 19871-19874 IN denotes for
T6432 19875-19877 IN denotes by
T6433 19878-19887 JJ denotes technical
T6434 19888-19894 NN denotes issues
T6435 19895-19897 IN denotes in
T6436 19898-19909 NN denotes stimulation
T6437 19910-19914 NN denotes time
T6438 19915-19918 CC denotes and
T6439 19919-19923 NN denotes dose
T6440 19925-19933 RB denotes Moreover
T6441 19933-19934 -COMMA- denotes ,
T6442 19935-19940 NN denotes IL-1β
T6443 19941-19951 NN denotes production
T6444 19952-19955 VB denotes was
T6445 19956-19959 RB denotes not
T6446 19960-19969 VB denotes increased
T6447 19970-19972 IN denotes in
T6448 19973-19975 NN denotes CF
T6449 19976-19981 NN denotes PBMCs
T6450 19982-19986 IN denotes with
T6451 19987-19999 JJ denotes inflammasome
T6452 20000-20011 NN denotes stimulation
T6453 20012-20017 RB denotes alone
T6454 20018-20020 RB denotes as
T6455 20021-20026 MD denotes would
T6456 20027-20029 VB denotes be
T6457 20030-20041 VB denotes anticipated
T6458 20042-20044 IN denotes if
T6459 20045-20050 EX denotes there
T6460 20051-20055 VB denotes were
T6461 20056-20061 JJ denotes basal
T6462 20062-20068 NN denotes levels
T6463 20069-20071 IN denotes of
T6464 20072-20077 NN denotes NF-κB
T6465 20078-20088 NN denotes activation
T6466 20090-20095 NN denotes Cells
T6467 20096-20105 JJ denotes deficient
T6468 20106-20108 IN denotes in
T6469 20109-20113 NN denotes CFTR
T6470 20114-20117 VB denotes are
T6471 20118-20125 VB denotes thought
T6472 20126-20128 TO denotes to
T6473 20129-20136 VB denotes exhibit
T6474 20137-20139 DT denotes an
T6475 20140-20149 VB denotes increased
T6476 20150-20155 JJ denotes basal
T6477 20156-20161 NN denotes level
T6478 20162-20164 IN denotes of
T6479 20165-20170 NN denotes NF-κB
T6480 20171-20179 NN denotes activity
T6481 20179-20180 -COMMA- denotes ,
T6482 20181-20186 WDT denotes which
T6483 20187-20192 VB denotes leads
T6484 20193-20195 TO denotes to
T6485 20196-20205 VB denotes increased
T6486 20206-20222 JJ denotes pro-inflammatory
T6487 20223-20231 NN denotes cytokine
T6488 20232-20242 NN denotes production
T6489 20243-20252 VB denotes including
T6490 20253-20255 DT denotes an
T6491 20256-20265 VB denotes increased
T6492 20266-20278 NN denotes availability
T6493 20279-20281 IN denotes of
T6494 20282-20291 NN denotes pro-IL-1β
T6495 20292-20295 IN denotes for
T6496 20296-20304 NN denotes cleavage
T6497 20305-20308 CC denotes and
T6498 20309-20318 NN denotes secretion
T6499 20320-20324 DT denotes This
T6500 20325-20338 NN denotes amplification
T6501 20339-20341 IN denotes of
T6502 20342-20347 NN denotes IL-1β
T6503 20348-20357 NN denotes secretion
T6504 20358-20361 VB denotes was
T6505 20362-20367 VB denotes shown
T6506 20368-20370 IN denotes by
T6507 20371-20378 VB denotes priming
T6508 20379-20384 NN denotes THP-1
T6509 20385-20394 NN denotes monocytes
T6510 20395-20398 CC denotes and
T6511 20399-20404 NN denotes PBMCs
T6512 20405-20409 IN denotes with
T6513 20410-20421 JJ denotes heat-killed
T6514 20422-20424 NNP denotes P.
T6515 20425-20435 NN denotes aeruginosa
T6516 20436-20438 CC denotes or
T6517 20439-20442 NN denotes LPS
T6518 20443-20448 JJ denotes prior
T6519 20449-20451 TO denotes to
T6520 20452-20463 NN denotes stimulation
T6521 20464-20468 IN denotes with
T6522 20469-20473 JJ denotes live
T6523 20474-20476 NNP denotes P.
T6524 20477-20487 NN denotes aeruginosa
T6525 20489-20493 DT denotes This
T6526 20494-20506 RB denotes dramatically
T6527 20507-20516 VB denotes increased
T6528 20517-20522 NN denotes IL-1β
T6529 20523-20532 NN denotes secretion
T6530 20533-20537 IN denotes over
T6531 20538-20549 NN denotes stimulation
T6532 20550-20554 IN denotes with
T6533 20555-20559 JJ denotes live
T6534 20560-20562 NNP denotes P.
T6535 20563-20573 NN denotes aeruginosa
T6536 20574-20581 IN denotes without
T6537 20582-20589 NN denotes priming
T6538 20590-20591 -LRB- denotes (
T6539 20591-20595 NN denotes Fig.
T6540 20596-20598 NN denotes 6c
T6541 20598-20599 -RRB- denotes )
T6542 20601-20610 RB denotes Similarly
T6543 20610-20611 -COMMA- denotes ,
T6544 20612-20614 IN denotes if
T6545 20615-20617 NN denotes CF
T6546 20618-20623 NN denotes PBMCs
T6547 20624-20633 VB denotes expressed
T6548 20634-20643 VB denotes increased
T6549 20644-20649 JJ denotes basal
T6550 20650-20655 NN denotes NF-κB
T6551 20656-20664 NN denotes activity
T6552 20664-20665 -COMMA- denotes ,
T6553 20666-20671 EX denotes there
T6554 20672-20677 MD denotes would
T6555 20678-20680 VB denotes be
T6556 20681-20683 DT denotes an
T6557 20684-20692 NN denotes increase
T6558 20693-20695 IN denotes in
T6559 20696-20701 NN denotes IL-1β
T6560 20702-20711 NN denotes secretion
T6561 20712-20714 IN denotes in
T6562 20715-20718 DT denotes the
T6563 20719-20726 NN denotes absence
T6564 20727-20729 IN denotes of
T6565 20730-20733 NN denotes LPS
T6566 20734-20741 NN denotes priming
T6567 20743-20750 RB denotes However
T6568 20750-20751 -COMMA- denotes ,
T6569 20752-20754 DT denotes no
T6570 20755-20763 NN denotes increase
T6571 20764-20766 IN denotes in
T6572 20767-20772 NN denotes IL-1β
T6573 20773-20776 VB denotes was
T6574 20777-20785 VB denotes observed
T6575 20786-20791 IN denotes under
T6576 20792-20797 JJ denotes basal
T6577 20798-20800 CC denotes or
T6578 20801-20807 VB denotes primed
T6579 20808-20818 NN denotes conditions
T6580 20820-20827 NN denotes Studies
T6581 20828-20841 VB denotes investigating
T6582 20842-20845 DT denotes the
T6583 20846-20856 NN denotes production
T6584 20857-20859 IN denotes of
T6585 20860-20865 NN denotes IL-1β
T6586 20866-20870 VB denotes have
T6587 20871-20875 VB denotes been
T6588 20876-20884 RB denotes somewhat
T6589 20885-20897 JJ denotes inconsistent
T6590 20899-20900 DT denotes A
T6591 20901-20906 NN denotes study
T6592 20907-20909 IN denotes by
T6593 20910-20919 NNP denotes Reininger
T6594 20920-20922 FW denotes et
T6595 20923-20926 FW denotes al.
T6596 20927-20928 -LRB- denotes [
T6597 20928-20930 CD denotes 43
T6598 20930-20931 -RRB- denotes ]
T6599 20932-20940 VB denotes provided
T6600 20941-20949 NN denotes evidence
T6601 20950-20954 IN denotes that
T6602 20955-20960 JJ denotes human
T6603 20961-20970 JJ denotes bronchial
T6604 20971-20981 JJ denotes epithelial
T6605 20982-20987 NN denotes cells
T6606 20988-20998 VB denotes possessing
T6607 20999-21002 DT denotes the
T6608 21003-21008 NN denotes ΔF508
T6609 21009-21013 NN denotes CFTR
T6610 21014-21022 NN denotes mutation
T6611 21023-21026 VB denotes had
T6612 21027-21028 DT denotes a
T6613 21029-21037 RB denotes slightly
T6614 21038-21045 VB denotes reduced
T6615 21046-21054 NN denotes capacity
T6616 21055-21057 TO denotes to
T6617 21058-21065 VB denotes produce
T6618 21066-21071 NN denotes IL-1β
T6619 21072-21075 CC denotes and
T6620 21076-21082 VB denotes lacked
T6621 21083-21086 DT denotes the
T6622 21087-21094 NN denotes ability
T6623 21095-21097 TO denotes to
T6624 21098-21104 VB denotes induce
T6625 21105-21107 DT denotes an
T6626 21108-21113 JJ denotes early
T6627 21114-21119 NN denotes NF-κB
T6628 21120-21130 NN denotes activation
T6629 21131-21133 IN denotes in
T6630 21134-21142 NN denotes response
T6631 21143-21145 TO denotes to
T6632 21146-21148 NNP denotes P.
T6633 21149-21159 NN denotes aeruginosa
T6634 21161-21171 RB denotes Conversely
T6635 21171-21172 -COMMA- denotes ,
T6636 21173-21174 DT denotes a
T6637 21175-21180 NN denotes study
T6638 21181-21183 IN denotes by
T6639 21184-21192 NNP denotes Kotrange
T6640 21193-21195 FW denotes et
T6641 21196-21199 FW denotes al.
T6642 21200-21201 -LRB- denotes [
T6643 21201-21203 CD denotes 31
T6644 21203-21204 -RRB- denotes ]
T6645 21205-21210 VB denotes found
T6646 21211-21215 IN denotes that
T6647 21216-21222 JJ denotes murine
T6648 21223-21227 NN denotes bone
T6649 21228-21242 JJ denotes marrow-derived
T6650 21243-21254 NN denotes macrophages
T6651 21255-21265 VB denotes expressing
T6652 21266-21276 NN denotes ΔF508-CFTR
T6653 21277-21285 VB denotes produced
T6654 21286-21295 VB denotes increased
T6655 21296-21303 NN denotes amounts
T6656 21304-21306 IN denotes of
T6657 21307-21312 NN denotes IL-1β
T6658 21313-21317 WRB denotes when
T6659 21318-21326 VB denotes compared
T6660 21327-21329 TO denotes to
T6661 21330-21341 NN denotes macrophages
T6662 21342-21352 VB denotes expressing
T6663 21353-21359 JJ denotes normal
T6664 21360-21364 NN denotes CFTR
T6665 21365-21367 IN denotes in
T6666 21368-21376 NN denotes response
T6667 21377-21379 TO denotes to
T6668 21380-21392 NNP denotes Burkholderia
T6669 21393-21404 NN denotes cenocepacia
T6670 21405-21410 NN denotes K56-2
T6671 21412-21415 DT denotes The
T6672 21416-21431 NN denotes differentiation
T6673 21432-21434 IN denotes of
T6674 21435-21444 NN denotes monocytes
T6675 21445-21449 IN denotes into
T6676 21450-21461 NN denotes macrophages
T6677 21462-21465 MD denotes may
T6678 21466-21472 RB denotes partly
T6679 21473-21480 VB denotes account
T6680 21481-21484 IN denotes for
T6681 21485-21488 DT denotes the
T6682 21489-21500 NN denotes differences
T6683 21501-21509 VB denotes observed
T6684 21510-21514 IN denotes with
T6685 21515-21518 DT denotes the
T6686 21519-21524 NN denotes study
T6687 21525-21527 IN denotes by
T6688 21528-21537 NNP denotes Kotrange.
T6689 21538-21559 JJ denotes Inflammasome-mediated
T6690 21560-21565 NN denotes IL-1β
T6691 21566-21576 NN denotes production
T6692 21577-21579 IN denotes by
T6693 21580-21589 NN denotes monocytes
T6694 21590-21593 CC denotes and
T6695 21594-21599 NN denotes PBMCs
T6696 21600-21604 VB denotes does
T6697 21605-21611 VB denotes differ
T6698 21612-21616 IN denotes from
T6699 21617-21628 NN denotes macrophages
T6700 21629-21630 -LRB- denotes [
T6701 21630-21632 CD denotes 47
T6702 21632-21633 -RRB- denotes ]
T6703 21633-21634 -COMMA- denotes ,
T6704 21635-21636 -LRB- denotes [
T6705 21636-21638 CD denotes 48
T6706 21638-21639 -RRB- denotes ]
T6707 21639-21640 -COMMA- denotes ,
T6708 21641-21642 -LRB- denotes [
T6709 21642-21644 CD denotes 49
T6710 21644-21645 -RRB- denotes ]
T6711 21646-21649 CC denotes and
T6712 21650-21661 NN denotes macrophages
T6713 21662-21665 VB denotes are
T6714 21666-21671 VB denotes found
T6715 21672-21674 TO denotes to
T6716 21675-21679 VB denotes have
T6717 21680-21686 JJ denotes higher
T6718 21687-21697 NN denotes expression
T6719 21698-21700 IN denotes of
T6720 21701-21705 NN denotes CFTR
T6721 21706-21710 IN denotes over
T6722 21711-21720 NN denotes monocytes
T6723 21721-21722 -LRB- denotes [
T6724 21722-21724 CD denotes 50
T6725 21724-21725 -RRB- denotes ]
T6726 21727-21734 RB denotes However
T6727 21734-21735 -COMMA- denotes ,
T6728 21736-21738 IN denotes as
T6729 21739-21748 NN denotes monocytes
T6730 21749-21752 CC denotes and
T6731 21753-21758 JJ denotes other
T6732 21759-21764 NN denotes PBMCs
T6733 21765-21772 VB denotes express
T6734 21773-21777 NNP denotes CFTR
T6735 21778-21779 -LRB- denotes [
T6736 21779-21781 CD denotes 50
T6737 21781-21782 -RRB- denotes ]
T6738 21782-21783 -COMMA- denotes ,
T6739 21784-21785 -LRB- denotes [
T6740 21785-21787 CD denotes 51
T6741 21787-21788 -RRB- denotes ]
T6742 21789-21792 CC denotes and
T6743 21793-21800 VB denotes produce
T6744 21801-21806 JJ denotes large
T6745 21807-21814 NN denotes amounts
T6746 21815-21817 IN denotes of
T6747 21818-21823 NN denotes IL-1β
T6748 21823-21824 -COMMA- denotes ,
T6749 21825-21829 PRP denotes they
T6750 21830-21833 VB denotes are
T6751 21834-21842 JJ denotes adequate
T6752 21843-21849 NN denotes models
T6753 21850-21852 TO denotes to
T6754 21853-21860 VB denotes examine
T6755 21861-21864 DT denotes the
T6756 21865-21872 NN denotes effects
T6757 21873-21875 IN denotes of
T6758 21876-21880 NN denotes CFTR
T6759 21881-21889 NN denotes function
T6760 21890-21892 IN denotes on
T6761 21893-21898 NN denotes IL-1β
T6762 21899-21909 NN denotes production
T6763 21911-21916 JJ denotes Other
T6764 21917-21930 JJ denotes hematopoeitic
T6765 21931-21936 NN denotes cells
T6766 21937-21940 MD denotes may
T6767 21941-21945 RB denotes also
T6768 21946-21956 VB denotes contribute
T6769 21957-21959 TO denotes to
T6770 21960-21965 NN denotes IL-1β
T6771 21966-21976 NN denotes production
T6772 21978-21981 IN denotes For
T6773 21982-21989 NN denotes example
T6774 21989-21990 -COMMA- denotes ,
T6775 21991-22001 NN denotes neutrophil
T6776 22002-22008 NN denotes counts
T6777 22009-22012 MD denotes can
T6778 22013-22015 VB denotes be
T6779 22016-22029 RB denotes significantly
T6780 22030-22039 VB denotes increased
T6781 22040-22042 IN denotes in
T6782 22043-22046 DT denotes the
T6783 22047-22052 NN denotes lungs
T6784 22053-22055 IN denotes of
T6785 22056-22058 NN denotes CF
T6786 22059-22067 NN denotes patients
T6787 22068-22069 -LRB- denotes [
T6788 22069-22071 CD denotes 52
T6789 22071-22072 -RRB- denotes ]
T6790 22072-22073 -COMMA- denotes ,
T6791 22074-22075 -LRB- denotes [
T6792 22075-22077 CD denotes 53
T6793 22077-22078 -RRB- denotes ]
T6794 22078-22079 -COMMA- denotes ,
T6795 22080-22081 -LRB- denotes [
T6796 22081-22083 CD denotes 54
T6797 22083-22084 -RRB- denotes ]
T6798 22085-22088 CC denotes and
T6799 22089-22092 MD denotes may
T6800 22093-22100 VB denotes produce
T6801 22101-22107 JJ denotes mature
T6802 22108-22113 NN denotes IL-1β
T6803 22114-22121 IN denotes through
T6804 22122-22131 NN denotes caspase-1
T6805 22132-22143 JJ denotes independent
T6806 22144-22154 NN denotes mechanisms
T6807 22155-22156 -LRB- denotes [
T6808 22156-22158 CD denotes 55
T6809 22158-22159 -RRB- denotes ]
T6810 22161-22164 DT denotes The
T6811 22165-22169 NN denotes role
T6812 22170-22172 IN denotes of
T6813 22173-22178 NN denotes NF-κB
T6814 22179-22189 NN denotes activation
T6815 22190-22192 IN denotes in
T6816 22193-22205 JJ denotes inflammasome
T6817 22206-22216 NN denotes activation
T6818 22217-22220 CC denotes and
T6819 22221-22226 NN denotes IL-1β
T6820 22227-22236 NN denotes secretion
T6821 22237-22239 VB denotes is
T6822 22240-22243 RB denotes not
T6823 22244-22259 JJ denotes straightforward
T6824 22261-22268 NN denotes Studies
T6825 22269-22273 VB denotes have
T6826 22274-22282 VB denotes revealed
T6827 22283-22285 DT denotes an
T6828 22286-22295 JJ denotes essential
T6829 22296-22300 NN denotes role
T6830 22301-22304 IN denotes for
T6831 22305-22310 NN denotes NF-κB
T6832 22311-22321 NN denotes activation
T6833 22322-22324 IN denotes in
T6834 22325-22328 DT denotes the
T6835 22329-22339 NN denotes production
T6836 22340-22342 IN denotes of
T6837 22343-22352 JJ denotes pro-IL-1β
T6838 22353-22356 CC denotes and
T6839 22357-22369 JJ denotes inflammasome
T6840 22370-22380 NN denotes components
T6841 22381-22385 JJ denotes such
T6842 22386-22388 IN denotes as
T6843 22389-22394 NN denotes NLRP3
T6844 22395-22396 -LRB- denotes [
T6845 22396-22398 CD denotes 22
T6846 22398-22399 -RRB- denotes ]
T6847 22399-22400 -COMMA- denotes ,
T6848 22401-22402 -LRB- denotes [
T6849 22402-22404 CD denotes 23
T6850 22404-22405 -RRB- denotes ]
T6851 22407-22409 IN denotes In
T6852 22410-22418 NN denotes contrast
T6853 22418-22419 -COMMA- denotes ,
T6854 22420-22428 NN denotes deletion
T6855 22429-22431 IN denotes of
T6856 22432-22436 NN denotes IKKβ
T6857 22436-22437 -COMMA- denotes ,
T6858 22438-22439 DT denotes a
T6859 22440-22446 NN denotes kinase
T6860 22447-22456 JJ denotes essential
T6861 22457-22459 IN denotes in
T6862 22460-22465 NN denotes NF-κB
T6863 22466-22476 NN denotes activation
T6864 22476-22477 -COMMA- denotes ,
T6865 22478-22487 VB denotes increases
T6866 22488-22493 NN denotes IL-1β
T6867 22494-22503 NN denotes secretion
T6868 22504-22506 IN denotes in
T6869 22507-22513 JJ denotes murine
T6870 22514-22525 NN denotes macrophages
T6871 22526-22527 -LRB- denotes [
T6872 22527-22529 CD denotes 56
T6873 22529-22530 -RRB- denotes ]
T6874 22530-22531 -COMMA- denotes ,
T6875 22532-22533 -LRB- denotes [
T6876 22533-22535 CD denotes 57
T6877 22535-22536 -RRB- denotes ]
T6878 22537-22540 CC denotes and
T6879 22541-22553 VB denotes demonstrates
T6880 22554-22555 DT denotes a
T6881 22556-22560 JJ denotes dual
T6882 22561-22565 NN denotes role
T6883 22566-22569 IN denotes for
T6884 22570-22575 NN denotes NF-κB
T6885 22576-22578 IN denotes in
T6886 22579-22589 NN denotes regulation
T6887 22590-22592 IN denotes of
T6888 22593-22598 NN denotes IL-1β
T6889 22600-22602 TO denotes To
T6890 22603-22610 VB denotes address
T6891 22611-22615 DT denotes this
T6892 22616-22627 NN denotes uncertainty
T6893 22628-22630 IN denotes in
T6894 22631-22634 PRP-DOLLAR- denotes our
T6895 22635-22646 NN denotes experiments
T6896 22647-22649 PRP denotes we
T6897 22650-22654 RB denotes also
T6898 22655-22665 VB denotes quantified
T6899 22666-22670 NN denotes IL-8
T6900 22670-22671 -COMMA- denotes ,
T6901 22672-22674 DT denotes an
T6902 22675-22684 JJ denotes important
T6903 22685-22687 NN denotes CF
T6904 22688-22696 NN denotes cytokine
T6905 22697-22700 CC denotes and
T6906 22701-22707 NN denotes marker
T6907 22708-22710 IN denotes of
T6908 22711-22716 NN denotes NF-κB
T6909 22717-22727 NN denotes activation
T6910 22728-22729 -LRB- denotes [
T6911 22729-22731 CD denotes 58
T6912 22731-22732 -RRB- denotes ]
T6913 22732-22733 -COMMA- denotes ,
T6914 22734-22737 CC denotes and
T6915 22738-22743 VB denotes found
T6916 22744-22746 DT denotes no
T6917 22747-22758 NN denotes differences
T6918 22759-22766 IN denotes between
T6919 22767-22769 NN denotes CF
T6920 22770-22773 CC denotes and
T6921 22774-22781 JJ denotes control
T6922 22782-22790 NN denotes subjects
T6923 22792-22801 RB denotes Similarly
T6924 22801-22802 -COMMA- denotes ,
T6925 22803-22809 NN denotes levels
T6926 22810-22812 IN denotes of
T6927 22813-22826 JJ denotes intracellular
T6928 22827-22836 NN denotes pro-IL-1β
T6929 22837-22839 IN denotes in
T6930 22840-22845 NN denotes THP-1
T6931 22846-22851 NN denotes cells
T6932 22852-22856 VB denotes were
T6933 22857-22866 JJ denotes dependent
T6934 22867-22869 IN denotes on
T6935 22870-22875 NN denotes NF-κB
T6936 22876-22884 NN denotes activity
T6937 22885-22888 CC denotes and
T6938 22889-22892 VB denotes did
T6939 22893-22896 RB denotes not
T6940 22897-22905 VB denotes increase
T6941 22906-22910 IN denotes with
T6942 22911-22921 NN denotes CFTRinh172
T6943 22922-22931 NN denotes treatment
T6944 22933-22943 JJ denotes Subsequent
T6945 22944-22953 NN denotes treatment
T6946 22954-22956 IN denotes of
T6947 22957-22962 NN denotes THP-1
T6948 22963-22968 NN denotes cells
T6949 22969-22972 CC denotes and
T6950 22973-22978 NN denotes PBMCs
T6951 22979-22983 IN denotes with
T6952 22984-22987 DT denotes the
T6953 22988-22993 NN denotes NF-κB
T6954 22994-23003 NN denotes inhibitor
T6955 23004-23014 NN denotes Bay11-7082
T6956 23015-23028 RB denotes significantly
T6957 23029-23038 VB denotes inhibited
T6958 23039-23043 CC denotes both
T6959 23044-23049 NN denotes IL-1β
T6960 23050-23053 CC denotes and
T6961 23054-23058 NN denotes IL-8
T6962 23059-23068 NN denotes secretion
T6963 23069-23070 -LRB- denotes (
T6964 23070-23074 NNP denotes Fig.
T6965 23075-23079 CD denotes 6d–h
T6966 23079-23080 -RRB- denotes )
T6967 23082-23091 RB denotes Therefore
T6968 23091-23092 -COMMA- denotes ,
T6969 23093-23096 DT denotes the
T6970 23097-23102 NN denotes IL-1β
T6971 23103-23106 CC denotes and
T6972 23107-23111 NN denotes IL-8
T6973 23112-23121 NN denotes responses
T6974 23122-23130 VB denotes observed
T6975 23131-23135 VB denotes were
T6976 23136-23140 DT denotes both
T6977 23141-23150 JJ denotes dependent
T6978 23151-23155 IN denotes upon
T6979 23156-23161 NN denotes NF-κB
T6980 23162-23172 NN denotes activation
T6981 23174-23181 VB denotes Priming
T6982 23182-23186 IN denotes with
T6983 23187-23198 JJ denotes heat-killed
T6984 23199-23201 NNP denotes P.
T6985 23202-23212 NN denotes aeruginosa
T6986 23212-23213 -COMMA- denotes ,
T6987 23214-23218 IN denotes like
T6988 23219-23222 NN denotes LPS
T6989 23222-23223 -COMMA- denotes ,
T6990 23224-23226 VB denotes is
T6991 23227-23233 JJ denotes unable
T6992 23234-23236 TO denotes to
T6993 23237-23243 VB denotes induce
T6994 23244-23245 DT denotes a
T6995 23246-23252 JJ denotes strong
T6996 23253-23258 NN denotes IL-1β
T6997 23259-23267 NN denotes response
T6998 23268-23270 IN denotes as
T6999 23271-23279 VB denotes compared
T7000 23280-23282 TO denotes to
T7001 23283-23287 VB denotes live
T7002 23288-23290 NNP denotes P.
T7003 23291-23301 NNP denotes aeruginosa
T7004 23302-23303 -LRB- denotes (
T7005 23303-23307 NNP denotes Fig.
T7006 23308-23310 NN denotes 6c
T7007 23310-23311 -RRB- denotes )
T7008 23311-23312 -COMMA- denotes ,
T7009 23313-23316 CC denotes but
T7010 23317-23326 VB denotes generated
T7011 23327-23334 JJ denotes greater
T7012 23335-23345 NN denotes NF-κB/AP-1
T7013 23346-23356 NN denotes activation
T7014 23357-23358 -LRB- denotes (
T7015 23358-23362 NN denotes Fig.
T7016 23363-23365 NN denotes 6a
T7017 23365-23366 -RRB- denotes )
T7018 23367-23370 CC denotes and
T7019 23371-23375 NN denotes IL-8
T7020 23376-23385 NN denotes secretion
T7021 23386-23387 -LRB- denotes (
T7022 23387-23391 NN denotes Fig.
T7023 23392-23394 NN denotes 6b
T7024 23394-23395 -RRB- denotes )
T7025 23396-23400 IN denotes than
T7026 23401-23405 JJ denotes live
T7027 23406-23414 NN denotes bacteria
T7028 23415-23422 IN denotes despite
T7029 23423-23434 NN denotes stimulation
T7030 23435-23437 IN denotes at
T7031 23438-23448 JJ denotes equivalent
T7032 23449-23453 NN denotes MOIs
T7033 23455-23459 DT denotes This
T7034 23460-23463 MD denotes may
T7035 23464-23466 VB denotes be
T7036 23467-23477 JJ denotes indicative
T7037 23478-23480 IN denotes of
T7038 23481-23484 DT denotes the
T7039 23485-23494 JJ denotes different
T7040 23495-23501 NN denotes degree
T7041 23502-23505 CC denotes and
T7042 23506-23513 NN denotes quality
T7043 23514-23516 IN denotes of
T7044 23517-23520 DT denotes the
T7045 23521-23533 JJ denotes inflammatory
T7046 23534-23542 NN denotes response
T7047 23543-23552 VB denotes generated
T7048 23553-23555 IN denotes by
T7049 23556-23560 JJ denotes live
T7050 23561-23563 IN denotes as
T7051 23564-23571 VB denotes opposed
T7052 23572-23574 TO denotes to
T7053 23575-23579 JJ denotes dead
T7054 23580-23588 NN denotes bacteria
T7055 23589-23590 -LRB- denotes [
T7056 23590-23592 CD denotes 59
T7057 23592-23593 -RRB- denotes ]
T7058 23595-23604 JJ denotes Potential
T7059 23605-23617 NN denotes shortcomings
T7060 23618-23620 IN denotes of
T7061 23621-23626 DT denotes these
T7062 23627-23638 NN denotes experiments
T7063 23639-23646 VB denotes include
T7064 23647-23650 PRP-DOLLAR- denotes its
T7065 23651-23666 NN denotes translatability
T7066 23667-23669 TO denotes to
T7067 23670-23674 NN denotes lung
T7068 23675-23682 NN denotes disease
T7069 23683-23686 CC denotes and
T7070 23687-23693 NN denotes issues
T7071 23694-23701 VB denotes related
T7072 23702-23704 TO denotes to
T7073 23705-23708 DT denotes the
T7074 23709-23724 NN denotes hypermutability
T7075 23725-23727 IN denotes of
T7076 23728-23730 NNP denotes P.
T7077 23731-23741 NN denotes aeruginosa
T7078 23742-23748 IN denotes during
T7079 23749-23752 DT denotes the
T7080 23753-23762 NN denotes evolution
T7081 23763-23765 IN denotes of
T7082 23766-23773 JJ denotes chronic
T7083 23774-23783 NN denotes infection
T7084 23785-23793 IN denotes Although
T7085 23794-23797 DT denotes the
T7086 23798-23807 NN denotes responses
T7087 23808-23816 VB denotes measured
T7088 23817-23819 IN denotes in
T7089 23820-23830 JJ denotes peripheral
T7090 23831-23836 NN denotes blood
T7091 23837-23842 NN denotes cells
T7092 23843-23846 MD denotes may
T7093 23847-23850 RB denotes not
T7094 23851-23861 RB denotes completely
T7095 23862-23869 VB denotes reflect
T7096 23870-23873 DT denotes the
T7097 23874-23883 NN denotes responses
T7098 23884-23893 VB denotes occurring
T7099 23894-23896 IN denotes in
T7100 23897-23900 DT denotes the
T7101 23901-23903 NN denotes CF
T7102 23904-23908 NN denotes lung
T7103 23908-23909 -COMMA- denotes ,
T7104 23910-23915 NN denotes PBMCs
T7105 23916-23920 VB denotes have
T7106 23921-23922 DT denotes a
T7107 23923-23929 NN denotes number
T7108 23930-23932 IN denotes of
T7109 23933-23939 JJ denotes useful
T7110 23940-23950 NN denotes advantages
T7111 23950-23951 -COLON- denotes :
T7112 23952-23953 -LRB- denotes (
T7113 23953-23954 LS denotes i
T7114 23954-23955 -RRB- denotes )
T7115 23956-23961 NN denotes PBMCs
T7116 23962-23965 VB denotes are
T7117 23966-23969 RB denotes not
T7118 23970-23977 JJ denotes subject
T7119 23978-23980 TO denotes to
T7120 23981-23992 NN denotes alterations
T7121 23993-23997 WDT denotes that
T7122 23998-24001 MD denotes may
T7123 24002-24008 VB denotes emerge
T7124 24009-24013 IN denotes from
T7125 24014-24023 JJ denotes long-term
T7126 24024-24028 NN denotes cell
T7127 24029-24036 NN denotes culture
T7128 24036-24037 -COMMA- denotes ,
T7129 24038-24045 NN denotes cloning
T7130 24046-24049 CC denotes and
T7131 24050-24065 NN denotes immortalization
T7132 24065-24066 -COMMA- denotes ,
T7133 24067-24070 CC denotes and
T7134 24071-24072 -LRB- denotes (
T7135 24072-24074 LS denotes ii
T7136 24074-24075 -RRB- denotes )
T7137 24076-24081 NN denotes PBMCs
T7138 24082-24089 VB denotes express
T7139 24090-24091 DT denotes a
T7140 24092-24097 JJ denotes large
T7141 24098-24108 NN denotes repertoire
T7142 24109-24111 IN denotes of
T7143 24112-24118 JJ denotes innate
T7144 24119-24125 JJ denotes immune
T7145 24126-24135 NN denotes receptors
T7146 24136-24139 CC denotes and
T7147 24140-24147 VB denotes secrete
T7148 24148-24149 DT denotes a
T7149 24150-24155 JJ denotes broad
T7150 24156-24161 NN denotes array
T7151 24162-24164 IN denotes of
T7152 24165-24174 NN denotes cytokines
T7153 24175-24178 CC denotes and
T7154 24179-24189 NN denotes chemokines
T7155 24190-24198 VB denotes allowing
T7156 24199-24212 JJ denotes comprehensive
T7157 24213-24221 NN denotes analysis
T7158 24222-24224 IN denotes of
T7159 24225-24228 DT denotes the
T7160 24229-24239 NN denotes modulation
T7161 24240-24242 IN denotes of
T7162 24243-24255 JJ denotes inflammatory
T7163 24256-24265 NN denotes responses
T7164 24266-24268 IN denotes by
T7165 24269-24273 NN denotes CFTR
T7166 24275-24288 NN denotes Consideration
T7167 24289-24293 MD denotes must
T7168 24294-24298 RB denotes also
T7169 24299-24301 VB denotes be
T7170 24302-24307 VB denotes given
T7171 24308-24310 TO denotes to
T7172 24311-24314 DT denotes the
T7173 24315-24321 NN denotes nature
T7174 24322-24324 IN denotes of
T7175 24325-24327 NNP denotes P.
T7176 24328-24338 NN denotes aeruginosa
T7177 24339-24348 NN denotes infection
T7178 24349-24352 CC denotes and
T7179 24353-24362 JJ denotes genotypic
T7180 24363-24370 NN denotes changes
T7181 24371-24373 IN denotes in
T7182 24374-24376 NNP denotes P.
T7183 24377-24387 NN denotes aeruginosa
T7184 24388-24390 IN denotes as
T7185 24391-24400 NN denotes infection
T7186 24401-24411 NN denotes progresses
T7187 24413-24415 NNP denotes P.
T7188 24416-24426 NN denotes aeruginosa
T7189 24427-24435 VB denotes mediates
T7190 24436-24448 JJ denotes inflammasome
T7191 24449-24459 NN denotes activation
T7192 24460-24467 IN denotes through
T7193 24468-24471 PRP-DOLLAR- denotes its
T7194 24472-24476 NN denotes type
T7195 24477-24480 CD denotes III
T7196 24481-24490 NN denotes secretion
T7197 24491-24497 NN denotes system
T7198 24498-24499 -LRB- denotes (
T7199 24499-24503 NN denotes T3SS
T7200 24503-24504 -RRB- denotes )
T7201 24505-24508 CC denotes and
T7202 24509-24512 DT denotes the
T7203 24513-24518 NN denotes NLRC4
T7204 24519-24531 NN denotes inflammasome
T7205 24532-24533 -LRB- denotes [
T7206 24533-24535 CD denotes 19
T7207 24535-24536 -RRB- denotes ]
T7208 24536-24537 -COMMA- denotes ,
T7209 24538-24539 -LRB- denotes [
T7210 24539-24541 CD denotes 20
T7211 24541-24542 -RRB- denotes ]
T7212 24544-24551 RB denotes However
T7213 24551-24552 -COMMA- denotes ,
T7214 24553-24559 NN denotes clones
T7215 24560-24562 IN denotes of
T7216 24563-24565 NNP denotes P.
T7217 24566-24576 NN denotes aeruginosa
T7218 24577-24588 VB denotes established
T7219 24589-24595 IN denotes during
T7220 24596-24603 JJ denotes chronic
T7221 24604-24613 NN denotes infection
T7222 24614-24617 MD denotes may
T7223 24618-24628 VB denotes accumulate
T7224 24629-24638 NN denotes mutations
T7225 24639-24641 IN denotes in
T7226 24642-24651 NN denotes virulence
T7227 24652-24659 NN denotes factors
T7228 24660-24664 JJ denotes such
T7229 24665-24667 IN denotes as
T7230 24668-24672 NN denotes exsA
T7231 24673-24674 -LRB- denotes [
T7232 24674-24676 CD denotes 60
T7233 24676-24677 -RRB- denotes ]
T7234 24679-24681 IN denotes By
T7235 24682-24691 VB denotes employing
T7236 24692-24693 DT denotes a
T7237 24694-24702 NN denotes deletion
T7238 24703-24709 NN denotes mutant
T7239 24710-24712 IN denotes in
T7240 24713-24717 NN denotes exsA
T7241 24717-24718 -COMMA- denotes ,
T7242 24719-24722 DT denotes the
T7243 24723-24726 JJ denotes key
T7244 24727-24736 NN denotes regulator
T7245 24737-24739 IN denotes in
T7246 24740-24744 NN denotes T3SS
T7247 24745-24758 NN denotes transcription
T7248 24758-24759 -COMMA- denotes ,
T7249 24760-24762 PRP denotes we
T7250 24763-24772 VB denotes confirmed
T7251 24773-24777 IN denotes that
T7252 24778-24781 DT denotes the
T7253 24782-24786 NN denotes T3SS
T7254 24787-24789 VB denotes is
T7255 24790-24799 JJ denotes important
T7256 24800-24803 IN denotes for
T7257 24804-24809 NN denotes IL-1β
T7258 24810-24819 NN denotes secretion
T7259 24820-24821 -LRB- denotes (
T7260 24821-24825 NN denotes Fig.
T7261 24826-24830 NN denotes 5a–b
T7262 24830-24831 -RRB- denotes )
T7263 24831-24832 -COMMA- denotes ,
T7264 24833-24836 CC denotes and
T7265 24837-24841 IN denotes that
T7266 24842-24851 VB denotes depending
T7267 24852-24854 IN denotes on
T7268 24855-24858 DT denotes the
T7269 24859-24869 NN denotes adaptation
T7270 24870-24872 IN denotes in
T7271 24873-24877 NN denotes type
T7272 24878-24881 CD denotes III
T7273 24882-24891 NN denotes secretion
T7274 24891-24892 -COMMA- denotes ,
T7275 24893-24896 DT denotes the
T7276 24897-24901 NN denotes host
T7277 24902-24907 NN denotes IL-1β
T7278 24908-24916 NN denotes response
T7279 24917-24920 MD denotes may
T7280 24921-24923 VB denotes be
T7281 24924-24926 RB denotes up
T7282 24927-24929 CC denotes or
T7283 24930-24943 VB denotes downregulated
T7284 24944-24945 -LRB- denotes [
T7285 24945-24947 CD denotes 19
T7286 24947-24948 -RRB- denotes ]
T7287 24948-24949 -COMMA- denotes ,
T7288 24950-24951 -LRB- denotes [
T7289 24951-24953 CD denotes 60
T7290 24953-24954 -RRB- denotes ]
T7291 24954-24955 -COMMA- denotes ,
T7292 24956-24957 -LRB- denotes [
T7293 24957-24959 CD denotes 61
T7294 24959-24960 -RRB- denotes ]
T7295 24962-24964 IN denotes In
T7296 24965-24975 NN denotes conclusion
T7297 24975-24976 -COMMA- denotes ,
T7298 24977-24980 PRP-DOLLAR- denotes our
T7299 24981-24985 NN denotes data
T7300 24986-24989 VB denotes are
T7301 24990-25000 JJ denotes consistent
T7302 25001-25005 IN denotes with
T7303 25006-25007 DT denotes a
T7304 25008-25012 NN denotes role
T7305 25013-25016 IN denotes for
T7306 25017-25030 JJ denotes hematopoietic
T7307 25031-25036 NN denotes cells
T7308 25036-25037 -COMMA- denotes ,
T7309 25038-25041 RB denotes not
T7310 25042-25048 VB denotes airway
T7311 25049-25059 JJ denotes epithelial
T7312 25060-25065 NN denotes cells
T7313 25065-25066 -COMMA- denotes ,
T7314 25067-25069 IN denotes as
T7315 25070-25073 DT denotes the
T7316 25074-25079 JJ denotes major
T7317 25080-25086 NN denotes source
T7318 25087-25089 IN denotes of
T7319 25090-25111 JJ denotes inflammasome-mediated
T7320 25112-25117 NN denotes IL-1β
T7321 25118-25128 NN denotes production
T7322 25129-25131 IN denotes in
T7323 25132-25135 DT denotes the
T7324 25136-25141 NN denotes lungs
T7325 25142-25144 IN denotes in
T7326 25145-25153 NN denotes response
T7327 25154-25156 TO denotes to
T7328 25157-25160 NN denotes ATP
T7329 25161-25164 CC denotes and
T7330 25165-25167 NNP denotes P.
T7331 25168-25178 NNP denotes aeruginosa
T7332 25180-25191 RB denotes Furthermore
T7333 25191-25192 -COMMA- denotes ,
T7334 25193-25195 PRP denotes we
T7335 25196-25200 VB denotes find
T7336 25201-25207 JJ denotes little
T7337 25208-25216 NN denotes evidence
T7338 25217-25219 TO denotes to
T7339 25220-25227 VB denotes support
T7340 25228-25230 DT denotes an
T7341 25231-25240 VB denotes increased
T7342 25241-25246 NN denotes IL-1β
T7343 25247-25259 JJ denotes inflammatory
T7344 25260-25268 NN denotes response
T7345 25269-25271 TO denotes to
T7346 25272-25290 JJ denotes NF-κB/Inflammasome
T7347 25291-25302 NN denotes stimulation
T7348 25303-25305 IN denotes in
T7349 25306-25308 NN denotes CF
T7350 25309-25317 NN denotes patients
T7351 25319-25326 JJ denotes Further
T7352 25327-25334 NN denotes studies
T7353 25335-25338 VB denotes are
T7354 25339-25348 VB denotes warranted
T7355 25349-25351 TO denotes to
T7356 25352-25361 VB denotes determine
T7357 25362-25364 IN denotes if
T7358 25365-25376 NN denotes adaptations
T7359 25377-25379 IN denotes of
T7360 25380-25382 NNP denotes P.
T7361 25383-25393 NN denotes aeruginosa
T7362 25394-25400 IN denotes during
T7363 25401-25404 DT denotes the
T7364 25405-25411 NN denotes course
T7365 25412-25414 IN denotes of
T7366 25415-25422 JJ denotes chronic
T7367 25423-25427 NN denotes lung
T7368 25428-25437 NN denotes infection
T7369 25438-25444 VB denotes alters
T7370 25445-25457 JJ denotes inflammasome
T7371 25458-25468 NN denotes activation
T7372 25468-25469 -COMMA- denotes ,
T7373 25470-25473 CC denotes and
T7374 25474-25481 IN denotes whether
T7375 25482-25486 DT denotes this
T7376 25487-25490 MD denotes can
T7377 25491-25493 VB denotes be
T7378 25494-25504 VB denotes correlated
T7379 25505-25509 IN denotes with
T7380 25510-25517 NN denotes disease
T7381 25518-25526 NN denotes severity
T7382 25527-25529 IN denotes in
T7383 25530-25532 NN denotes CF
T9313 25558-25564 NNP denotes Ethics
T9314 25565-25574 NNP denotes Statement
T9315 25575-25580 NN denotes Blood
T9316 25581-25588 NN denotes samples
T9317 25589-25593 VB denotes were
T9318 25594-25602 VB denotes obtained
T9319 25603-25607 IN denotes with
T9320 25608-25616 VB denotes informed
T9321 25617-25624 VB denotes written
T9322 25625-25632 NN denotes consent
T9323 25633-25637 IN denotes from
T9324 25638-25645 NN denotes control
T9325 25646-25654 NN denotes subjects
T9326 25655-25658 CC denotes and
T9327 25659-25661 NN denotes CF
T9328 25662-25670 NN denotes patients
T9329 25671-25673 IN denotes at
T9330 25674-25677 DT denotes the
T9331 25678-25680 NNP denotes BC
T9332 25681-25689 NNP denotes Children
T9333 25689-25691 POS denotes 's
T9334 25692-25701 NNP denotes Hospital.
T9335 25702-25709 NNP denotes Consent
T9336 25710-25713 VB denotes was
T9337 25714-25722 VB denotes obtained
T9338 25723-25726 IN denotes for
T9339 25727-25735 NN denotes children
T9340 25736-25738 IN denotes by
T9341 25739-25744 PRP-DOLLAR- denotes their
T9342 25745-25751 NN denotes parent
T9343 25752-25754 CC denotes or
T9344 25755-25760 JJ denotes legal
T9345 25761-25769 NN denotes guardian
T9346 25771-25779 NN denotes Subjects
T9347 25780-25781 CD denotes 7
T9348 25782-25787 NN denotes years
T9349 25788-25790 IN denotes of
T9350 25791-25794 NN denotes age
T9351 25795-25798 CC denotes and
T9352 25799-25804 JJ denotes older
T9353 25805-25809 VB denotes were
T9354 25810-25818 VB denotes required
T9355 25819-25821 TO denotes to
T9356 25822-25829 VB denotes provide
T9357 25830-25838 JJ denotes informed
T9358 25839-25845 NN denotes assent
T9359 25846-25848 RB denotes as
T9360 25849-25853 RB denotes well
T9361 25855-25864 NN denotes Protocols
T9362 25865-25869 VB denotes were
T9363 25870-25878 VB denotes approved
T9364 25879-25881 IN denotes by
T9365 25882-25885 DT denotes the
T9366 25886-25894 NNP denotes Clinical
T9367 25895-25903 NNP denotes Research
T9368 25904-25910 NNP denotes Ethics
T9369 25911-25916 NNP denotes Board
T9370 25917-25918 -LRB- denotes (
T9371 25918-25927 NN denotes H09-01192
T9372 25927-25928 -RRB- denotes )
T9469 25931-25935 NNP denotes Cell
T9470 25936-25943 NNP denotes Culture
T9471 25944-25946 NN denotes CF
T9472 25947-25948 -LRB- denotes (
T9473 25948-25953 NN denotes IB3-1
T9474 25954-25957 CC denotes and
T9475 25958-25964 NN denotes CuFi-1
T9476 25964-25965 -RRB- denotes )
T9477 25966-25969 CC denotes and
T9478 25970-25977 NN denotes control
T9479 25978-25979 -LRB- denotes (
T9480 25979-25981 NN denotes S9
T9481 25982-25985 CC denotes and
T9482 25986-25992 NN denotes NuLi-1
T9483 25992-25993 -RRB- denotes )
T9484 25994-25999 NN denotes cells
T9485 26000-26004 VB denotes were
T9486 26005-26013 VB denotes obtained
T9487 26014-26018 IN denotes from
T9488 26019-26022 DT denotes the
T9489 26023-26031 NNP denotes American
T9490 26032-26036 NNP denotes Type
T9491 26037-26044 NNP denotes Culture
T9492 26045-26056 NNP denotes Collection.
T9493 26057-26062 NN denotes IB3-1
T9494 26063-26068 NN denotes cells
T9495 26069-26073 VB denotes were
T9496 26074-26081 VB denotes derived
T9497 26082-26086 IN denotes from
T9498 26087-26088 DT denotes a
T9499 26089-26096 NN denotes patient
T9500 26097-26107 VB denotes expressing
T9501 26108-26111 DT denotes the
T9502 26112-26117 NN denotes ΔF508
T9503 26118-26121 CC denotes and
T9504 26122-26128 NN denotes W1282X
T9505 26129-26138 NN denotes mutations
T9506 26139-26142 CC denotes and
T9507 26143-26149 NN denotes CuFi-1
T9508 26150-26154 VB denotes were
T9509 26155-26162 VB denotes derived
T9510 26163-26167 IN denotes from
T9511 26168-26169 DT denotes a
T9512 26170-26175 NN denotes ΔF508
T9513 26176-26186 JJ denotes homozygous
T9514 26187-26194 NN denotes patient
T9515 26196-26198 NN denotes S9
T9516 26199-26204 NN denotes cells
T9517 26205-26208 VB denotes are
T9518 26209-26214 NN denotes IB3-1
T9519 26215-26220 NN denotes cells
T9520 26221-26225 WDT denotes that
T9521 26226-26230 VB denotes have
T9522 26231-26235 VB denotes been
T9523 26236-26247 VB denotes transfected
T9524 26248-26252 IN denotes with
T9525 26253-26257 NN denotes CFTR
T9526 26258-26263 VB denotes using
T9527 26264-26266 DT denotes an
T9528 26267-26283 JJ denotes adeno-associated
T9529 26284-26289 JJ denotes viral
T9530 26290-26296 NN denotes vector
T9531 26297-26300 CC denotes and
T9532 26301-26307 NN denotes NuLi-1
T9533 26308-26313 NN denotes cells
T9534 26314-26318 VB denotes were
T9535 26319-26326 VB denotes derived
T9536 26327-26331 IN denotes from
T9537 26332-26333 DT denotes a
T9538 26334-26341 NN denotes patient
T9539 26342-26352 VB denotes possessing
T9540 26353-26354 DT denotes a
T9541 26355-26364 JJ denotes wild-type
T9542 26365-26369 NN denotes CFTR
T9543 26370-26378 NN denotes genotype
T9544 26380-26390 JJ denotes THP1-XBlue
T9545 26391-26396 NN denotes cells
T9546 26397-26403 RB denotes stably
T9547 26404-26411 VB denotes express
T9548 26412-26413 DT denotes a
T9549 26414-26422 VB denotes secreted
T9550 26423-26432 JJ denotes embryonic
T9551 26433-26441 NN denotes alkaline
T9552 26442-26453 NN denotes phosphatase
T9553 26454-26455 -LRB- denotes (
T9554 26455-26459 NN denotes SEAP
T9555 26459-26460 -RRB- denotes )
T9556 26461-26469 NN denotes reporter
T9557 26470-26479 JJ denotes inducible
T9558 26480-26482 IN denotes by
T9559 26483-26488 NN denotes NF-κB
T9560 26489-26492 CC denotes and
T9561 26493-26497 NN denotes AP-1
T9562 26498-26499 -LRB- denotes (
T9563 26499-26508 NN denotes Invivogen
T9564 26508-26509 -RRB- denotes )
T9565 26511-26516 NN denotes Cells
T9566 26517-26521 VB denotes were
T9567 26522-26530 VB denotes cultured
T9568 26531-26533 IN denotes as
T9569 26534-26545 VB denotes recommended
T9570 26546-26548 IN denotes by
T9571 26549-26554 PRP-DOLLAR- denotes their
T9572 26555-26565 JJ denotes respective
T9573 26566-26575 NN denotes suppliers
T9574 26576-26581 VB denotes using
T9575 26582-26590 JJ denotes standard
T9576 26591-26600 NN denotes protocols
T9577 26602-26604 NN denotes S9
T9578 26605-26608 CC denotes and
T9579 26609-26614 NN denotes IB3-1
T9580 26615-26620 NN denotes cells
T9581 26621-26625 VB denotes were
T9582 26626-26634 VB denotes cultured
T9583 26635-26637 IN denotes in
T9584 26638-26643 JJ denotes basal
T9585 26644-26649 NN denotes LHC-8
T9586 26650-26651 -LRB- denotes (
T9587 26651-26661 NN denotes Invitrogen
T9588 26661-26662 -RRB- denotes )
T9589 26663-26675 VB denotes supplemented
T9590 26676-26680 IN denotes with
T9591 26681-26683 CD denotes 10
T9592 26683-26684 NN denotes %
T9593 26685-26686 -LRB- denotes (
T9594 26686-26689 NN denotes v/v
T9595 26689-26690 -RRB- denotes )
T9596 26691-26696 JJ denotes fetal
T9597 26697-26703 JJ denotes bovine
T9598 26704-26709 NN denotes serum
T9599 26710-26711 -LRB- denotes (
T9600 26711-26714 NN denotes FBS
T9601 26714-26715 -RRB- denotes )
T9602 26715-26716 -COMMA- denotes ,
T9603 26717-26718 CD denotes 2
T9604 26719-26721 NN denotes mM
T9605 26722-26733 NN denotes L-glutamine
T9606 26733-26734 -COMMA- denotes ,
T9607 26735-26738 CC denotes and
T9608 26739-26740 CD denotes 1
T9609 26741-26743 NN denotes mM
T9610 26744-26750 NN denotes sodium
T9611 26751-26759 NN denotes pyruvate
T9612 26761-26774 NN denotes NuLi-1/CuFi-1
T9613 26775-26780 NN denotes cells
T9614 26781-26785 VB denotes were
T9615 26786-26794 VB denotes cultured
T9616 26795-26797 IN denotes in
T9617 26798-26802 NN denotes BEBM
T9618 26803-26813 JJ denotes serum-free
T9619 26814-26820 NN denotes medium
T9620 26821-26822 -LRB- denotes (
T9621 26822-26827 NN denotes Lonza
T9622 26827-26828 -RRB- denotes )
T9623 26829-26833 IN denotes with
T9624 26834-26844 NN denotes supplement
T9625 26845-26851 NN denotes bullet
T9626 26852-26855 NN denotes kit
T9627 26856-26857 -LRB- denotes (
T9628 26857-26860 NN denotes EGF
T9629 26860-26861 -COMMA- denotes ,
T9630 26862-26876 NN denotes hydrocortisone
T9631 26876-26877 -COMMA- denotes ,
T9632 26878-26884 JJ denotes bovine
T9633 26885-26894 JJ denotes pituitary
T9634 26895-26902 NN denotes extract
T9635 26902-26903 -COMMA- denotes ,
T9636 26904-26915 NN denotes transferrin
T9637 26915-26916 -COMMA- denotes ,
T9638 26917-26923 JJ denotes bovine
T9639 26924-26931 NN denotes insulin
T9640 26931-26932 -COMMA- denotes ,
T9641 26933-26949 NN denotes triiodothyronine
T9642 26949-26950 -COMMA- denotes ,
T9643 26951-26962 NN denotes epinephrine
T9644 26962-26963 -COMMA- denotes ,
T9645 26964-26972 JJ denotes retinoic
T9646 26973-26977 NN denotes acid
T9647 26977-26978 -RRB- denotes )
T9648 26978-26979 -COMMA- denotes ,
T9649 26980-26981 CD denotes 2
T9650 26982-26984 NN denotes mM
T9651 26985-26996 NN denotes L-glutamine
T9652 26996-26997 -COMMA- denotes ,
T9653 26998-27001 CC denotes and
T9654 27002-27003 CD denotes 1
T9655 27004-27006 NN denotes mM
T9656 27007-27013 NN denotes sodium
T9657 27014-27022 NN denotes pyruvate
T9658 27024-27029 NN denotes PBMCs
T9659 27030-27034 IN denotes from
T9660 27035-27037 NN denotes CF
T9661 27038-27046 NN denotes patients
T9662 27047-27050 CC denotes and
T9663 27051-27059 NN denotes controls
T9664 27060-27064 VB denotes were
T9665 27065-27073 VB denotes cultured
T9666 27074-27076 IN denotes in
T9667 27077-27086 NN denotes RPMI-1640
T9668 27087-27088 -LRB- denotes (
T9669 27088-27095 NN denotes Hyclone
T9670 27095-27096 -RRB- denotes )
T9671 27097-27109 VB denotes supplemented
T9672 27110-27114 IN denotes with
T9673 27115-27117 CD denotes 10
T9674 27117-27118 NN denotes %
T9675 27119-27122 NNP denotes FBS
T9676 27122-27123 -COMMA- denotes ,
T9677 27124-27125 CD denotes 2
T9678 27126-27128 NN denotes mM
T9679 27129-27140 NN denotes L-glutamine
T9680 27140-27141 -COMMA- denotes ,
T9681 27142-27145 CC denotes and
T9682 27146-27147 CD denotes 1
T9683 27148-27150 NN denotes mM
T9684 27151-27157 NN denotes sodium
T9685 27158-27166 NN denotes pyruvate
T9686 27167-27168 -LRB- denotes (
T9687 27168-27176 JJ denotes complete
T9688 27177-27181 NN denotes RPMI
T9689 27181-27182 -RRB- denotes )
T9690 27184-27194 JJ denotes THP1-XBlue
T9691 27195-27200 NN denotes cells
T9692 27201-27205 VB denotes were
T9693 27206-27214 VB denotes cultured
T9694 27215-27217 IN denotes in
T9695 27218-27226 JJ denotes complete
T9696 27227-27231 NN denotes RPMI
T9697 27232-27236 IN denotes with
T9698 27237-27240 DT denotes the
T9699 27241-27249 NN denotes addition
T9700 27250-27252 IN denotes of
T9701 27253-27259 NN denotes zeocin
T9702 27260-27261 -LRB- denotes (
T9703 27261-27264 CD denotes 100
T9704 27265-27270 NN denotes µg/ml
T9705 27270-27271 -RRB- denotes )
T9706 27272-27274 TO denotes to
T9707 27275-27281 VB denotes select
T9708 27282-27285 IN denotes for
T9709 27286-27291 NN denotes cells
T9710 27292-27302 VB denotes expressing
T9711 27303-27306 DT denotes the
T9712 27307-27311 NN denotes SEAP
T9713 27312-27322 NN denotes NF-κB/AP-1
T9714 27323-27331 NN denotes reporter
T9715 27333-27338 RB denotes Prior
T9716 27339-27341 TO denotes to
T9717 27342-27353 NN denotes stimulation
T9718 27353-27354 -COMMA- denotes ,
T9719 27355-27364 JJ denotes bronchial
T9720 27365-27375 JJ denotes epithelial
T9721 27376-27380 NN denotes cell
T9722 27381-27386 NN denotes lines
T9723 27387-27391 VB denotes were
T9724 27392-27398 VB denotes plated
T9725 27399-27401 IN denotes in
T9726 27402-27408 VB denotes coated
T9727 27409-27410 -LRB- denotes [
T9728 27410-27412 CD denotes 62
T9729 27412-27413 -RRB- denotes ]
T9730 27414-27421 JJ denotes 96-well
T9731 27422-27428 NN denotes plates
T9732 27429-27430 -LRB- denotes (
T9733 27430-27432 NN denotes BD
T9734 27433-27444 NNP denotes Biosciences
T9735 27444-27445 -RRB- denotes )
T9736 27446-27448 IN denotes at
T9737 27449-27454 CD denotes 3×104
T9738 27455-27465 NN denotes cells/well
T9739 27466-27472 IN denotes unless
T9740 27473-27482 VB denotes indicated
T9741 27482-27483 -COMMA- denotes ,
T9742 27484-27487 CC denotes and
T9743 27488-27495 VB denotes allowed
T9744 27496-27498 TO denotes to
T9745 27499-27505 VB denotes adhere
T9746 27506-27515 RB denotes overnight
T9747 27517-27523 NN denotes Plates
T9748 27524-27527 IN denotes for
T9749 27528-27530 NN denotes S9
T9750 27531-27534 CC denotes and
T9751 27535-27540 NN denotes IB3-1
T9752 27541-27553 NN denotes stimulations
T9753 27554-27558 VB denotes were
T9754 27559-27565 VB denotes coated
T9755 27566-27568 IN denotes in
T9756 27569-27570 DT denotes a
T9757 27571-27578 NN denotes mixture
T9758 27579-27581 IN denotes of
T9759 27582-27588 JJ denotes bovine
T9760 27589-27594 NN denotes serum
T9761 27595-27602 NN denotes albumin
T9762 27603-27604 -LRB- denotes (
T9763 27604-27607 CD denotes 100
T9764 27608-27613 NN denotes µg/ml
T9765 27613-27614 -RRB- denotes )
T9766 27614-27615 -COMMA- denotes ,
T9767 27616-27627 NN denotes fibronectin
T9768 27628-27629 -LRB- denotes (
T9769 27629-27631 CD denotes 10
T9770 27632-27637 NN denotes µg/ml
T9771 27637-27638 -RRB- denotes )
T9772 27638-27639 -COMMA- denotes ,
T9773 27640-27643 CC denotes and
T9774 27644-27650 JJ denotes bovine
T9775 27651-27658 NN denotes collage
T9776 27659-27663 NN denotes type
T9777 27664-27665 CD denotes I
T9778 27666-27667 -LRB- denotes (
T9779 27667-27669 CD denotes 30
T9780 27670-27675 NN denotes µg/ml
T9781 27675-27676 -RRB- denotes )
T9782 27677-27678 -LRB- denotes (
T9783 27678-27680 NN denotes BD
T9784 27681-27692 NNP denotes Biosciences
T9785 27692-27693 -RRB- denotes )
T9786 27695-27701 NN denotes Plates
T9787 27702-27705 IN denotes for
T9788 27706-27712 NN denotes NuLi-1
T9789 27713-27716 CC denotes and
T9790 27717-27723 NN denotes CuFi-1
T9791 27724-27728 VB denotes were
T9792 27729-27735 VB denotes coated
T9793 27736-27740 IN denotes with
T9794 27741-27749 NN denotes collagen
T9795 27750-27754 NN denotes type
T9796 27755-27757 CD denotes IV
T9797 27758-27759 -LRB- denotes (
T9798 27759-27761 CD denotes 60
T9799 27762-27767 NN denotes µg/ml
T9800 27767-27768 -RRB- denotes )
T9801 27769-27770 -LRB- denotes (
T9802 27770-27775 NNP denotes Sigma
T9803 27776-27783 NNP denotes Aldrich
T9804 27783-27784 -RRB- denotes )
T9805 27786-27791 NN denotes PBMCs
T9806 27792-27796 VB denotes were
T9807 27797-27803 VB denotes plated
T9808 27804-27806 IN denotes in
T9809 27807-27814 JJ denotes 96-well
T9810 27815-27821 NN denotes plates
T9811 27822-27824 IN denotes at
T9812 27825-27826 DT denotes a
T9813 27827-27834 NN denotes density
T9814 27835-27837 IN denotes of
T9815 27838-27845 CD denotes 1.5×105
T9816 27846-27856 NN denotes cells/well
T9817 27857-27859 IN denotes in
T9818 27860-27863 CD denotes 200
T9819 27864-27866 NN denotes µl
T9820 27867-27868 -LRB- denotes (
T9821 27868-27875 CD denotes 7.5×105
T9822 27876-27884 NN denotes cells/mL
T9823 27884-27885 -RRB- denotes )
T9824 27887-27892 NN denotes THP-1
T9825 27893-27901 NN denotes reporter
T9826 27902-27907 NN denotes cells
T9827 27908-27912 VB denotes were
T9828 27913-27927 VB denotes differentiated
T9829 27928-27932 IN denotes into
T9830 27933-27934 DT denotes a
T9831 27935-27950 JJ denotes macrophage-like
T9832 27951-27960 NN denotes phenotype
T9833 27961-27966 VB denotes using
T9834 27967-27969 CD denotes 50
T9835 27970-27975 NN denotes ng/ml
T9836 27976-27978 IN denotes of
T9837 27979-27986 NN denotes phorbol
T9838 27987-27999 NN denotes 12-myristate
T9839 28000-28010 NN denotes 13-acetate
T9840 28011-28012 -LRB- denotes (
T9841 28012-28015 NN denotes PMA
T9842 28015-28016 -RRB- denotes )
T9843 28017-28018 -LRB- denotes (
T9844 28018-28023 NNP denotes Sigma
T9845 28024-28031 NNP denotes Aldrich
T9846 28031-28032 -RRB- denotes )
T9847 28033-28036 IN denotes for
T9848 28037-28039 CD denotes 24
T9849 28040-28045 NN denotes hours
T9850 28046-28048 IN denotes at
T9851 28049-28050 DT denotes a
T9852 28051-28058 NN denotes density
T9853 28059-28061 IN denotes of
T9854 28062-28067 CD denotes 1×105
T9855 28068-28078 NN denotes cells/well
T9856 28079-28081 IN denotes in
T9857 28082-28085 CD denotes 200
T9858 28086-28088 NN denotes µl
T9859 28089-28090 -LRB- denotes (
T9860 28090-28095 CD denotes 5×105
T9861 28096-28104 NN denotes cells/ml
T9862 28104-28105 -RRB- denotes )
T9863 28107-28112 NN denotes Cells
T9864 28113-28117 VB denotes were
T9865 28118-28124 VB denotes washed
T9866 28125-28129 IN denotes with
T9867 28130-28133 NNP denotes PBS
T9868 28134-28137 CC denotes and
T9869 28138-28145 VB denotes allowed
T9870 28146-28148 TO denotes to
T9871 28149-28153 VB denotes rest
T9872 28154-28155 DT denotes a
T9873 28156-28163 JJ denotes further
T9874 28164-28166 CD denotes 42
T9875 28167-28172 NN denotes hours
T9876 28173-28178 RB denotes prior
T9877 28179-28181 TO denotes to
T9878 28182-28193 NN denotes stimulation
T10424 28196-28198 NN denotes CF
T10425 28199-28202 CC denotes and
T10426 28203-28210 NN denotes control
T10427 28211-28218 NN denotes subject
T10428 28219-28224 NN denotes PBMCs
T10429 28225-28228 DT denotes The
T10430 28229-28238 NN denotes diagnosis
T10431 28239-28241 IN denotes of
T10432 28242-28244 NN denotes CF
T10433 28245-28248 VB denotes was
T10434 28249-28260 VB denotes established
T10435 28261-28263 IN denotes by
T10436 28264-28271 JJ denotes typical
T10437 28272-28280 JJ denotes clinical
T10438 28281-28289 NN denotes features
T10439 28289-28290 -COMMA- denotes ,
T10440 28291-28300 VB denotes increased
T10441 28301-28306 NN denotes sweat
T10442 28307-28315 NN denotes chloride
T10443 28316-28330 NN denotes concentrations
T10444 28331-28332 -LRB- denotes (
T10445 28332-28333 SYM denotes >
T10446 28333-28335 CD denotes 60
T10447 28336-28342 NN denotes mmol/l
T10448 28342-28343 -RRB- denotes )
T10449 28343-28344 -COMMA- denotes ,
T10450 28345-28348 CC denotes and
T10451 28349-28358 NN denotes detection
T10452 28359-28361 IN denotes of
T10453 28362-28373 JJ denotes CF-inducing
T10454 28374-28383 NN denotes mutations
T10455 28385-28388 DT denotes All
T10456 28389-28397 NN denotes patients
T10457 28398-28402 IN denotes with
T10458 28403-28405 NN denotes CF
T10459 28406-28410 VB denotes were
T10460 28411-28421 RB denotes clinically
T10461 28422-28428 JJ denotes stable
T10462 28429-28431 IN denotes at
T10463 28432-28435 DT denotes the
T10464 28436-28440 NN denotes time
T10465 28441-28443 IN denotes of
T10466 28444-28449 NN denotes blood
T10467 28450-28458 NN denotes donation
T10468 28458-28459 -COMMA- denotes ,
T10469 28460-28463 CC denotes and
T10470 28464-28466 PRP denotes we
T10471 28467-28475 VB denotes excluded
T10472 28476-28479 DT denotes any
T10473 28480-28488 NN denotes subjects
T10474 28489-28492 WP denotes who
T10475 28493-28497 VB denotes were
T10476 28498-28507 VB denotes receiving
T10477 28508-28516 JJ denotes systemic
T10478 28517-28532 NN denotes corticosteroids
T10479 28533-28536 JJ denotes due
T10480 28537-28539 TO denotes to
T10481 28540-28549 JJ denotes potential
T10482 28550-28566 JJ denotes immunomodulatory
T10483 28567-28575 NN denotes activity
T10484 28577-28584 NN denotes Control
T10485 28585-28592 NN denotes samples
T10486 28593-28597 VB denotes were
T10487 28598-28606 VB denotes provided
T10488 28607-28609 IN denotes by
T10489 28610-28617 JJ denotes healthy
T10490 28618-28623 JJ denotes adult
T10491 28624-28634 NN denotes volunteers
T10492 28636-28638 IN denotes In
T10493 28639-28649 RB denotes previously
T10494 28650-28659 VB denotes published
T10495 28660-28664 NN denotes work
T10496 28665-28667 PRP denotes we
T10497 28668-28672 VB denotes have
T10498 28673-28685 VB denotes demonstrated
T10499 28686-28690 IN denotes that
T10500 28691-28703 JJ denotes TLR-mediated
T10501 28704-28716 JJ denotes inflammatory
T10502 28717-28726 NN denotes responses
T10503 28727-28730 VB denotes are
T10504 28731-28737 JJ denotes stable
T10505 28738-28740 IN denotes in
T10506 28741-28747 NN denotes humans
T10507 28748-28752 IN denotes from
T10508 28753-28758 NN denotes birth
T10509 28759-28761 TO denotes to
T10510 28762-28764 CD denotes 60
T10511 28765-28770 NN denotes years
T10512 28771-28774 JJ denotes old
T10513 28775-28776 -LRB- denotes [
T10514 28776-28778 CD denotes 63
T10515 28778-28779 -RRB- denotes ]
T10516 28779-28780 -COMMA- denotes ,
T10517 28781-28790 RB denotes therefore
T10518 28791-28794 VB denotes did
T10519 28795-28798 RB denotes not
T10520 28799-28808 VB denotes age-match
T10521 28809-28812 DT denotes the
T10522 28813-28815 NN denotes CF
T10523 28816-28824 NN denotes patients
T10524 28825-28828 CC denotes and
T10525 28829-28836 JJ denotes control
T10526 28837-28845 NN denotes subjects
T10527 28847-28857 JJ denotes Peripheral
T10528 28858-28863 NN denotes blood
T10529 28864-28867 VB denotes was
T10530 28868-28877 VB denotes collected
T10531 28878-28880 IN denotes in
T10532 28881-28887 NN denotes sodium
T10533 28888-28895 NN denotes heparin
T10534 28896-28901 NN denotes tubes
T10535 28902-28903 -LRB- denotes (
T10536 28903-28905 NN denotes BD
T10537 28906-28917 NNP denotes Biosciences
T10538 28917-28918 -RRB- denotes )
T10539 28919-28922 CC denotes and
T10540 28923-28928 NN denotes PBMCs
T10541 28929-28933 VB denotes were
T10542 28934-28942 VB denotes isolated
T10543 28943-28948 VB denotes using
T10544 28949-28956 NN denotes density
T10545 28957-28965 NN denotes gradient
T10546 28966-28980 NN denotes centrifugation
T10547 28981-28983 IN denotes on
T10548 28984-28997 JJ denotes Ficoll-Paque™
T10549 28998-29002 NN denotes Plus
T10550 29003-29004 -LRB- denotes (
T10551 29004-29006 NNP denotes GE
T10552 29007-29017 NNP denotes Healthcare
T10553 29017-29018 -RRB- denotes )
T10554 29020-29023 DT denotes The
T10555 29024-29029 NN denotes layer
T10556 29030-29040 VB denotes containing
T10557 29041-29046 NN denotes PBMCs
T10558 29047-29050 VB denotes was
T10559 29051-29059 VB denotes isolated
T10560 29059-29060 -COMMA- denotes ,
T10561 29061-29067 VB denotes washed
T10562 29068-29073 RB denotes twice
T10563 29074-29076 IN denotes in
T10564 29077-29080 NNP denotes PBS
T10565 29081-29084 CC denotes and
T10566 29085-29096 VB denotes resuspended
T10567 29097-29099 IN denotes in
T10568 29100-29108 JJ denotes complete
T10569 29109-29113 NN denotes RPMI
T10570 29115-29120 NN denotes Cells
T10571 29121-29125 VB denotes were
T10572 29126-29136 VB denotes enumerated
T10573 29137-29139 IN denotes by
T10574 29140-29146 NN denotes trypan
T10575 29147-29151 JJ denotes blue
T10576 29152-29161 NN denotes exclusion
T10577 29162-29167 VB denotes using
T10578 29168-29171 DT denotes the
T10579 29172-29180 NNP denotes Countess
T10580 29181-29190 VB denotes automated
T10581 29191-29195 NN denotes cell
T10582 29196-29203 NN denotes counter
T10583 29204-29205 -LRB- denotes (
T10584 29205-29215 NN denotes Invitrogen
T10585 29215-29216 -RRB- denotes )
T10586 29218-29221 IN denotes For
T10587 29222-29232 NN denotes derivation
T10588 29233-29235 IN denotes of
T10589 29236-29247 NN denotes macrophages
T10590 29248-29252 IN denotes from
T10591 29253-29262 NN denotes monocytes
T10592 29262-29263 -COMMA- denotes ,
T10593 29264-29273 NN denotes monocytes
T10594 29274-29278 VB denotes were
T10595 29279-29286 VB denotes allowed
T10596 29287-29289 TO denotes to
T10597 29290-29296 VB denotes adhere
T10598 29297-29299 TO denotes to
T10599 29300-29307 NN denotes plastic
T10600 29308-29311 IN denotes for
T10601 29312-29313 CD denotes 2
T10602 29314-29319 NN denotes hours
T10603 29320-29322 IN denotes in
T10604 29323-29327 NN denotes RPMI
T10605 29328-29332 CD denotes 1640
T10606 29333-29338 IN denotes after
T10607 29339-29344 WDT denotes which
T10608 29345-29357 JJ denotes non-adherent
T10609 29358-29363 NN denotes cells
T10610 29364-29368 VB denotes were
T10611 29369-29376 VB denotes removed
T10612 29378-29387 NN denotes Monocytes
T10613 29388-29392 VB denotes were
T10614 29393-29400 VB denotes allowed
T10615 29401-29403 TO denotes to
T10616 29404-29417 VB denotes differentiate
T10617 29418-29420 IN denotes in
T10618 29421-29425 NN denotes RPMI
T10619 29426-29430 CD denotes 1640
T10620 29431-29443 VB denotes supplemented
T10621 29444-29448 IN denotes with
T10622 29449-29451 CD denotes 10
T10623 29451-29452 NN denotes %
T10624 29453-29458 JJ denotes human
T10625 29459-29461 NN denotes AB
T10626 29462-29467 NN denotes serum
T10627 29468-29471 IN denotes for
T10628 29472-29474 CD denotes 10
T10629 29475-29479 NN denotes days
T10872 29482-29486 NN denotes Cell
T10873 29487-29498 NN denotes stimulation
T10874 29499-29502 CC denotes and
T10875 29503-29511 NN denotes cytokine
T10876 29512-29526 NN denotes quantification
T10877 29527-29536 JJ denotes Bronchial
T10878 29537-29547 JJ denotes epithelial
T10879 29548-29553 NN denotes cells
T10880 29554-29558 VB denotes were
T10881 29559-29565 VB denotes plated
T10882 29566-29569 CC denotes and
T10883 29570-29577 VB denotes allowed
T10884 29578-29580 TO denotes to
T10885 29581-29587 VB denotes adhere
T10886 29588-29597 JJ denotes overnight
T10887 29598-29603 JJ denotes prior
T10888 29604-29606 TO denotes to
T10889 29607-29618 NN denotes stimulation
T10890 29620-29629 JJ denotes Bronchial
T10891 29630-29640 JJ denotes epithelial
T10892 29641-29646 NN denotes cells
T10893 29647-29651 VB denotes were
T10894 29652-29658 VB denotes rested
T10895 29659-29661 CC denotes or
T10896 29662-29668 VB denotes primed
T10897 29669-29673 IN denotes with
T10898 29674-29677 NN denotes LPS
T10899 29678-29681 IN denotes for
T10900 29682-29683 CD denotes 5
T10901 29684-29689 NN denotes hours
T10902 29690-29693 CC denotes and
T10903 29694-29704 VB denotes stimulated
T10904 29705-29709 IN denotes with
T10905 29710-29714 JJ denotes live
T10906 29715-29717 NNP denotes P.
T10907 29718-29728 NN denotes aeruginosa
T10908 29729-29733 NN denotes PAO1
T10909 29734-29736 CC denotes or
T10910 29737-29740 NN denotes ATP
T10911 29741-29744 IN denotes for
T10912 29745-29748 DT denotes the
T10913 29749-29754 NN denotes times
T10914 29755-29764 VB denotes indicated
T10915 29766-29771 NN denotes PBMCs
T10916 29772-29775 CC denotes and
T10917 29776-29781 NN denotes THP-1
T10918 29782-29790 NN denotes reporter
T10919 29791-29796 NN denotes cells
T10920 29797-29801 VB denotes were
T10921 29802-29808 CC denotes either
T10922 29809-29815 VB denotes rested
T10923 29816-29818 CC denotes or
T10924 29819-29825 VB denotes primed
T10925 29826-29830 IN denotes with
T10926 29831-29834 NN denotes LPS
T10927 29835-29836 -LRB- denotes (
T10928 29836-29845 NN denotes Invivogen
T10929 29845-29846 -RRB- denotes )
T10930 29847-29849 CC denotes or
T10931 29850-29861 JJ denotes heat-killed
T10932 29862-29866 NN denotes PAO1
T10933 29867-29876 NN denotes overnight
T10934 29877-29878 -LRB- denotes (
T10935 29878-29880 CD denotes 16
T10936 29881-29886 NN denotes hours
T10937 29886-29887 -RRB- denotes )
T10938 29889-29892 DT denotes The
T10939 29893-29897 JJ denotes next
T10940 29898-29901 NN denotes day
T10941 29902-29905 DT denotes the
T10942 29906-29911 NN denotes cells
T10943 29912-29916 VB denotes were
T10944 29917-29927 VB denotes challenged
T10945 29928-29932 IN denotes with
T10946 29933-29937 JJ denotes live
T10947 29938-29942 NN denotes PAO1
T10948 29942-29943 -COMMA- denotes ,
T10949 29944-29953 NN denotes PAO1ΔexsA
T10950 29953-29954 -COMMA- denotes ,
T10951 29955-29958 NN denotes ATP
T10952 29959-29960 -LRB- denotes (
T10953 29960-29969 NN denotes Invivogen
T10954 29969-29970 -RRB- denotes )
T10955 29970-29971 -COMMA- denotes ,
T10956 29972-29974 CC denotes or
T10957 29975-29979 NNP denotes Poly
T10958 29979-29980 -LRB- denotes (
T10959 29980-29982 NN denotes dA
T10960 29982-29983 -COLON- denotes :
T10961 29983-29985 NN denotes dT
T10962 29985-29986 -RRB- denotes )
T10963 29987-29988 -LRB- denotes (
T10964 29988-29993 NNP denotes Sigma
T10965 29994-30001 NNP denotes Aldrich
T10966 30001-30002 -RRB- denotes )
T10967 30003-30006 IN denotes for
T10968 30007-30010 DT denotes the
T10969 30011-30016 NN denotes times
T10970 30017-30026 VB denotes indicated
T10971 30027-30028 -LRB- denotes (
T10972 30028-30031 VB denotes see
T10973 30032-30036 NNP denotes Fig.
T10974 30037-30038 CD denotes 1
T10975 30038-30039 -RRB- denotes )
T10976 30041-30044 IN denotes For
T10977 30045-30057 NN denotes stimulations
T10978 30058-30062 IN denotes with
T10979 30063-30067 NN denotes Poly
T10980 30067-30068 -LRB- denotes (
T10981 30068-30070 NN denotes dA
T10982 30070-30071 -COLON- denotes :
T10983 30071-30073 NN denotes dT
T10984 30073-30074 -RRB- denotes )
T10985 30074-30075 -COMMA- denotes ,
T10986 30076-30089 NN denotes lipofectamine
T10987 30090-30093 NN denotes LTX
T10988 30094-30097 VB denotes was
T10989 30098-30102 VB denotes used
T10990 30103-30105 IN denotes at
T10991 30106-30107 DT denotes a
T10992 30108-30111 NN denotes 1∶1
T10993 30112-30113 -LRB- denotes (
T10994 30113-30116 NN denotes w∶v
T10995 30116-30117 -RRB- denotes )
T10996 30118-30123 NN denotes ratio
T10997 30124-30126 IN denotes of
T10998 30127-30129 NN denotes µg
T10999 30130-30132 IN denotes of
T11000 30133-30136 NN denotes DNA
T11001 30137-30139 TO denotes to
T11002 30140-30142 VB denotes µl
T11003 30143-30145 IN denotes of
T11004 30146-30159 NN denotes lipofectamine
T11005 30160-30163 CC denotes and
T11006 30164-30167 VB denotes was
T11007 30168-30173 VB denotes mixed
T11008 30174-30176 CD denotes 30
T11009 30177-30184 NN denotes minutes
T11010 30185-30190 RB denotes prior
T11011 30191-30193 TO denotes to
T11012 30194-30205 NN denotes stimulation
T11013 30207-30210 DT denotes The
T11014 30211-30216 NN denotes NF-κB
T11015 30217-30226 NN denotes inhibitor
T11016 30227-30237 NN denotes Bay11-7082
T11017 30238-30239 -LRB- denotes (
T11018 30239-30248 NN denotes Invivogen
T11019 30248-30249 -RRB- denotes )
T11020 30250-30253 VB denotes was
T11021 30254-30259 VB denotes added
T11022 30260-30262 TO denotes to
T11023 30263-30271 NN denotes cultures
T11024 30272-30273 CD denotes 1
T11025 30274-30278 NN denotes hour
T11026 30279-30284 JJ denotes prior
T11027 30285-30287 TO denotes to
T11028 30288-30295 NN denotes priming
T11029 30297-30299 IN denotes If
T11030 30300-30302 DT denotes no
T11031 30303-30310 NN denotes priming
T11032 30311-30314 VB denotes was
T11033 30315-30323 VB denotes involved
T11034 30323-30324 -COMMA- denotes ,
T11035 30325-30334 NN denotes inhibitor
T11036 30335-30338 VB denotes was
T11037 30339-30344 VB denotes added
T11038 30345-30346 CD denotes 1
T11039 30347-30351 NN denotes hour
T11040 30352-30357 JJ denotes prior
T11041 30358-30360 TO denotes to
T11042 30361-30373 JJ denotes inflammasome
T11043 30374-30385 NN denotes stimulation
T11044 30387-30390 DT denotes The
T11045 30391-30395 NNP denotes CFTR
T11046 30396-30405 NN denotes inhibitor
T11047 30406-30416 NN denotes CFTRinh172
T11048 30417-30418 -LRB- denotes (
T11049 30418-30423 NNP denotes Sigma
T11050 30424-30431 NNP denotes Aldrich
T11051 30431-30432 -RRB- denotes )
T11052 30433-30436 VB denotes was
T11053 30437-30442 VB denotes added
T11054 30443-30445 TO denotes to
T11055 30446-30454 NN denotes cultures
T11056 30455-30457 CD denotes 18
T11057 30458-30463 NN denotes hours
T11058 30464-30469 RB denotes prior
T11059 30470-30472 TO denotes to
T11060 30473-30485 JJ denotes inflammasome
T11061 30486-30497 NN denotes stimulation
T11062 30499-30502 DT denotes The
T11063 30503-30512 NN denotes caspase-1
T11064 30513-30522 NN denotes inhibitor
T11065 30523-30533 NN denotes z-YVAD-fmk
T11066 30534-30535 -LRB- denotes (
T11067 30535-30544 NN denotes Biovision
T11068 30544-30545 -RRB- denotes )
T11069 30546-30549 VB denotes was
T11070 30550-30555 VB denotes added
T11071 30556-30558 TO denotes to
T11072 30559-30567 NN denotes cultures
T11073 30568-30569 CD denotes 1
T11074 30570-30574 NN denotes hour
T11075 30575-30580 JJ denotes prior
T11076 30581-30583 TO denotes to
T11077 30584-30596 JJ denotes inflammasome
T11078 30597-30608 NN denotes stimulation
T11079 30610-30622 NN denotes Supernatants
T11080 30623-30627 VB denotes were
T11081 30628-30637 VB denotes collected
T11082 30638-30641 CC denotes and
T11083 30642-30648 VB denotes stored
T11084 30649-30651 IN denotes at
T11085 30652-30658 NNP denotes −20°C.
T11086 30659-30668 NNP denotes Cytokines
T11087 30669-30677 VB denotes released
T11088 30678-30682 IN denotes into
T11089 30683-30695 NN denotes supernatants
T11090 30696-30700 IN denotes from
T11091 30701-30706 NN denotes PBMCs
T11092 30707-30717 VB denotes stimulated
T11093 30718-30722 IN denotes with
T11094 30723-30735 JJ denotes inflammasome
T11095 30736-30746 NN denotes activators
T11096 30747-30751 VB denotes were
T11097 30752-30762 VB denotes quantified
T11098 30763-30768 VB denotes using
T11099 30769-30777 JJ denotes sandwich
T11100 30778-30783 NN denotes ELISA
T11101 30784-30785 -LRB- denotes (
T11102 30785-30796 NN denotes eBioscience
T11103 30796-30797 -RRB- denotes )
T11388 30800-30814 NN denotes Immunoblotting
T11389 30815-30820 CD denotes 1×106
T11390 30821-30826 NN denotes cells
T11391 30827-30831 VB denotes were
T11392 30832-30838 VB denotes seeded
T11393 30839-30841 IN denotes in
T11394 30842-30849 JJ denotes 12-well
T11395 30850-30856 NN denotes plates
T11396 30856-30857 -COMMA- denotes ,
T11397 30858-30868 VB denotes stimulated
T11398 30869-30871 IN denotes as
T11399 30872-30881 VB denotes indicated
T11400 30881-30882 -COMMA- denotes ,
T11401 30883-30886 CC denotes and
T11402 30887-30892 VB denotes lysed
T11403 30893-30895 IN denotes in
T11404 30896-30900 NN denotes RIPA
T11405 30901-30907 NN denotes buffer
T11406 30908-30920 VB denotes supplemented
T11407 30921-30925 IN denotes with
T11408 30926-30930 NN denotes Halt
T11409 30931-30939 NN denotes protease
T11410 30940-30943 CC denotes and
T11411 30944-30955 NN denotes phosphatase
T11412 30956-30965 NN denotes inhibitor
T11413 30966-30974 NN denotes cocktail
T11414 30975-30976 -LRB- denotes (
T11415 30976-30982 NNP denotes Thermo
T11416 30983-30993 NNP denotes Scientific
T11417 30993-30994 -RRB- denotes )
T11418 30996-31003 NN denotes Protein
T11419 31004-31018 NN denotes concentrations
T11420 31019-31023 VB denotes were
T11421 31024-31034 VB denotes determined
T11422 31035-31037 IN denotes by
T11423 31038-31046 NN denotes Bradford
T11424 31047-31052 NN denotes assay
T11425 31053-31054 -LRB- denotes (
T11426 31054-31060 NNP denotes Thermo
T11427 31061-31071 NNP denotes Scientific
T11428 31071-31072 -RRB- denotes )
T11429 31074-31081 NN denotes Lysates
T11430 31082-31086 VB denotes were
T11431 31087-31095 VB denotes resolved
T11432 31096-31098 IN denotes by
T11433 31099-31114 NN denotes electrophoreses
T11434 31115-31117 IN denotes on
T11435 31118-31120 CD denotes 10
T11436 31120-31121 NN denotes %
T11437 31122-31140 NN denotes SDS-polyacrylamide
T11438 31141-31145 NN denotes gels
T11439 31146-31149 CC denotes and
T11440 31150-31161 VB denotes transferred
T11441 31162-31166 IN denotes onto
T11442 31167-31171 NN denotes PVDF
T11443 31172-31181 NN denotes membranes
T11444 31182-31183 -LRB- denotes (
T11445 31183-31192 NNP denotes Millipore
T11446 31192-31193 -RRB- denotes )
T11447 31195-31200 NN denotes Blots
T11448 31201-31205 VB denotes were
T11449 31206-31213 VB denotes blocked
T11450 31214-31217 IN denotes for
T11451 31218-31219 CD denotes 1
T11452 31220-31224 NN denotes hour
T11453 31225-31227 IN denotes at
T11454 31228-31232 NN denotes room
T11455 31233-31244 NN denotes temperature
T11456 31245-31248 CC denotes and
T11457 31249-31255 VB denotes probed
T11458 31256-31265 RB denotes overnight
T11459 31266-31268 IN denotes at
T11460 31269-31272 NN denotes 4°C
T11461 31273-31276 IN denotes for
T11462 31277-31286 NN denotes pro-IL-1β
T11463 31287-31288 -LRB- denotes (
T11464 31288-31293 NNP denotes Santa
T11465 31294-31298 NNP denotes Cruz
T11466 31298-31299 -RRB- denotes )
T11467 31299-31300 -COMMA- denotes ,
T11468 31301-31305 NN denotes IκBα
T11469 31306-31307 -LRB- denotes (
T11470 31307-31311 NN denotes Cell
T11471 31312-31321 NN denotes Signaling
T11472 31321-31322 -RRB- denotes )
T11473 31322-31323 -COMMA- denotes ,
T11474 31324-31326 CC denotes or
T11475 31327-31334 NN denotes β-actin
T11476 31335-31336 -LRB- denotes (
T11477 31336-31340 NN denotes Cell
T11478 31341-31350 NN denotes Signaling
T11479 31350-31351 -RRB- denotes )
T11480 31353-31358 NN denotes Blots
T11481 31359-31363 VB denotes were
T11482 31364-31376 RB denotes subsequently
T11483 31377-31383 VB denotes probed
T11484 31384-31388 IN denotes with
T11485 31389-31410 JJ denotes fluorescently-labeled
T11486 31411-31420 JJ denotes secondary
T11487 31421-31431 NN denotes antibodies
T11488 31431-31432 -COMMA- denotes ,
T11489 31433-31439 NN denotes IRDye®
T11490 31440-31443 CD denotes 680
T11491 31444-31446 CC denotes or
T11492 31447-31452 NN denotes 800CW
T11493 31453-31454 -LRB- denotes (
T11494 31454-31460 NNP denotes LI-COR
T11495 31461-31472 NNP denotes Biosciences
T11496 31472-31473 -RRB- denotes )
T11497 31474-31477 IN denotes for
T11498 31478-31479 CD denotes 1
T11499 31480-31484 NN denotes hour
T11500 31486-31490 CC denotes Both
T11501 31491-31499 VB denotes blocking
T11502 31500-31503 CC denotes and
T11503 31504-31511 VB denotes probing
T11504 31512-31517 NN denotes steps
T11505 31518-31522 VB denotes were
T11506 31523-31530 VB denotes carried
T11507 31531-31534 RP denotes out
T11508 31535-31537 IN denotes in
T11509 31538-31551 JJ denotes tris-buffered
T11510 31552-31558 NN denotes saline
T11511 31559-31560 -LRB- denotes (
T11512 31560-31561 NNP denotes G
T11513 31562-31573 NNP denotes Biosciences
T11514 31573-31574 -RRB- denotes )
T11515 31575-31585 VB denotes containing
T11516 31586-31587 CD denotes 5
T11517 31587-31588 NN denotes %
T11518 31589-31595 JJ denotes bovine
T11519 31596-31601 NN denotes serum
T11520 31602-31609 NN denotes albumin
T11521 31610-31613 CC denotes and
T11522 31614-31617 CD denotes 0.1
T11523 31617-31618 NN denotes %
T11524 31619-31624 NN denotes TWEEN
T11525 31625-31627 CD denotes 20
T11526 31628-31629 -LRB- denotes (
T11527 31629-31639 NN denotes Calbiochem
T11528 31639-31640 -RRB- denotes )
T11529 31642-31647 NN denotes Blots
T11530 31648-31652 VB denotes were
T11531 31653-31659 VB denotes imaged
T11532 31660-31662 IN denotes on
T11533 31663-31664 DT denotes a
T11534 31665-31671 NNP denotes LI-COR
T11535 31672-31679 NNP denotes Odyssey
T11536 31680-31688 VB denotes infrared
T11537 31689-31696 NN denotes imaging
T11538 31697-31703 NN denotes system
T11539 31704-31705 -LRB- denotes (
T11540 31705-31711 NNP denotes LI-COR
T11541 31712-31723 NNP denotes Biosciences
T11542 31723-31724 -RRB- denotes )
T11543 31725-31728 CC denotes and
T11544 31729-31739 VB denotes quantified
T11545 31740-31745 VB denotes using
T11546 31746-31749 DT denotes the
T11547 31750-31758 VB denotes included
T11548 31759-31767 NN denotes analysis
T11549 31768-31776 NN denotes software
T11763 31779-31793 NN denotes Quantification
T11764 31794-31796 IN denotes of
T11765 31797-31806 NN denotes caspase-1
T11766 31807-31815 NN denotes activity
T11767 31816-31825 JJ denotes Bronchial
T11768 31826-31836 JJ denotes epithelial
T11769 31837-31842 NN denotes cells
T11770 31843-31847 VB denotes were
T11771 31848-31854 VB denotes plated
T11772 31855-31857 IN denotes in
T11773 31858-31864 JJ denotes 6-well
T11774 31865-31871 NN denotes plates
T11775 31872-31874 IN denotes at
T11776 31875-31880 CD denotes 5×105
T11777 31881-31891 NN denotes cells/well
T11778 31892-31901 RB denotes overnight
T11779 31903-31908 NN denotes Cells
T11780 31909-31913 VB denotes were
T11781 31914-31920 VB denotes primed
T11782 31921-31925 IN denotes with
T11783 31926-31929 NN denotes LPS
T11784 31930-31933 IN denotes for
T11785 31934-31935 CD denotes 5
T11786 31936-31941 NN denotes hours
T11787 31942-31945 CC denotes and
T11788 31946-31956 VB denotes stimulated
T11789 31957-31961 IN denotes with
T11790 31962-31965 NN denotes ATP
T11791 31966-31969 IN denotes for
T11792 31970-31971 CD denotes 1
T11793 31972-31976 NN denotes hour
T11794 31977-31979 CC denotes or
T11795 31980-31990 VB denotes stimulated
T11796 31991-31995 IN denotes with
T11797 31996-32000 JJ denotes live
T11798 32001-32005 NN denotes PAO1
T11799 32006-32009 IN denotes for
T11800 32010-32011 CD denotes 3
T11801 32012-32017 NN denotes hours
T11802 32019-32024 NN denotes PBMCs
T11803 32025-32029 VB denotes were
T11804 32030-32040 VB denotes stimulated
T11805 32041-32044 DT denotes the
T11806 32045-32049 JJ denotes same
T11807 32050-32053 NN denotes day
T11808 32054-32056 IN denotes as
T11809 32057-32062 NN denotes blood
T11810 32063-32071 NN denotes donation
T11811 32073-32078 NN denotes PBMCs
T11812 32079-32083 VB denotes were
T11813 32084-32090 VB denotes seeded
T11814 32091-32093 IN denotes in
T11815 32094-32095 DT denotes a
T11816 32096-32103 JJ denotes 96-well
T11817 32104-32109 NN denotes plate
T11818 32110-32112 IN denotes at
T11819 32113-32114 DT denotes a
T11820 32115-32122 NN denotes density
T11821 32123-32125 IN denotes of
T11822 32126-32133 CD denotes 4.5×105
T11823 32134-32144 NN denotes cells/well
T11824 32145-32146 -LRB- denotes (
T11825 32146-32153 CD denotes 2.5×106
T11826 32154-32162 NN denotes cells/ml
T11827 32162-32163 -RRB- denotes )
T11828 32163-32164 -COMMA- denotes ,
T11829 32165-32171 VB denotes primed
T11830 32172-32176 IN denotes with
T11831 32177-32180 NN denotes LPS
T11832 32181-32184 IN denotes for
T11833 32185-32186 CD denotes 5
T11834 32187-32192 NN denotes hours
T11835 32193-32196 CC denotes and
T11836 32197-32201 RB denotes then
T11837 32202-32212 VB denotes stimulated
T11838 32213-32217 IN denotes with
T11839 32218-32221 NN denotes ATP
T11840 32222-32225 IN denotes for
T11841 32226-32227 CD denotes 1
T11842 32228-32232 NN denotes hour
T11843 32233-32235 CC denotes or
T11844 32236-32240 NN denotes Poly
T11845 32240-32241 -LRB- denotes (
T11846 32241-32243 NN denotes dA
T11847 32243-32244 -COLON- denotes :
T11848 32244-32246 NN denotes dT
T11849 32246-32247 -RRB- denotes )
T11850 32248-32251 IN denotes for
T11851 32252-32253 CD denotes 3
T11852 32254-32259 NN denotes hours
T11853 32260-32262 CC denotes or
T11854 32263-32273 VB denotes stimulated
T11855 32274-32278 IN denotes with
T11856 32279-32283 JJ denotes live
T11857 32284-32288 NN denotes PAO1
T11858 32289-32292 IN denotes for
T11859 32293-32294 CD denotes 3
T11860 32295-32300 NN denotes hours
T11861 32302-32311 NN denotes Caspase-1
T11862 32312-32320 NN denotes activity
T11863 32321-32324 VB denotes was
T11864 32325-32333 VB denotes measured
T11865 32334-32339 VB denotes using
T11866 32340-32345 NN denotes FLICA
T11867 32346-32347 -LRB- denotes (
T11868 32347-32362 NNP denotes Immunochemistry
T11869 32363-32375 NNP denotes Technologies
T11870 32375-32376 -RRB- denotes )
T11871 32376-32377 -COMMA- denotes ,
T11872 32378-32379 DT denotes a
T11873 32380-32394 JJ denotes cell-permeable
T11874 32395-32406 JJ denotes fluorescent
T11875 32407-32412 NN denotes probe
T11876 32413-32414 -LRB- denotes (
T11877 32414-32426 NN denotes FAM-YVAD-fmk
T11878 32426-32427 -RRB- denotes )
T11879 32428-32432 WDT denotes that
T11880 32433-32438 VB denotes binds
T11881 32439-32445 JJ denotes active
T11882 32446-32455 NN denotes caspase-1
T11883 32457-32462 NN denotes Cells
T11884 32463-32467 VB denotes were
T11885 32468-32477 VB denotes incubated
T11886 32478-32479 CD denotes 1
T11887 32480-32484 NN denotes hour
T11888 32485-32489 IN denotes with
T11889 32490-32495 NN denotes FLICA
T11890 32496-32498 IN denotes at
T11891 32499-32503 NN denotes 37°C
T11892 32504-32507 CC denotes and
T11893 32508-32515 VB denotes stained
T11894 32516-32520 IN denotes with
T11895 32521-32538 JJ denotes PE-Cy7-conjugated
T11896 32539-32548 JJ denotes anti-CD14
T11897 32549-32559 NN denotes antibodies
T11898 32560-32561 -LRB- denotes (
T11899 32561-32572 NN denotes eBioscience
T11900 32572-32573 -RRB- denotes )
T11901 32574-32576 TO denotes to
T11902 32577-32585 VB denotes identify
T11903 32586-32595 NN denotes monocytes
T11904 32597-32600 DT denotes The
T11905 32601-32607 VB denotes gating
T11906 32608-32616 NN denotes strategy
T11907 32617-32626 VB denotes consisted
T11908 32627-32629 IN denotes of
T11909 32630-32639 VB denotes including
T11910 32640-32644 JJ denotes live
T11911 32645-32650 NN denotes cells
T11912 32651-32655 WDT denotes that
T11913 32656-32660 VB denotes were
T11914 32661-32665 NN denotes CD14
T11915 32666-32674 JJ denotes positive
T11916 32675-32680 WDT denotes which
T11917 32681-32685 VB denotes were
T11918 32686-32698 RB denotes subsequently
T11919 32699-32707 VB denotes analyzed
T11920 32708-32711 IN denotes for
T11921 32712-32715 DT denotes the
T11922 32716-32725 NN denotes frequency
T11923 32726-32728 IN denotes of
T11924 32729-32734 NN denotes FLICA
T11925 32735-32743 JJ denotes positive
T11926 32744-32749 NN denotes cells
T12143 32752-32762 NN denotes NF-κB/AP-1
T12144 32763-32771 NN denotes Activity
T12145 32772-32777 NN denotes Assay
T12146 32778-32790 NN denotes Supernatants
T12147 32791-32795 IN denotes from
T12148 32796-32801 NN denotes THP-1
T12149 32802-32810 NN denotes reporter
T12150 32811-32816 NN denotes cells
T12151 32817-32821 VB denotes were
T12152 32822-32831 VB denotes incubated
T12153 32832-32836 IN denotes with
T12154 32837-32848 JJ denotes Quanti-Blue
T12155 32849-32858 NN denotes substrate
T12156 32859-32860 -LRB- denotes (
T12157 32860-32869 NN denotes Invivogen
T12158 32869-32870 -RRB- denotes )
T12159 32871-32873 IN denotes at
T12160 32874-32878 NN denotes 37°C
T12161 32879-32882 CC denotes and
T12162 32883-32890 VB denotes allowed
T12163 32891-32893 TO denotes to
T12164 32894-32901 VB denotes develop
T12165 32902-32905 IN denotes for
T12166 32906-32911 CD denotes 16–18
T12167 32912-32917 NN denotes hours
T12168 32919-32930 NN denotes Quanti-Blue
T12169 32931-32939 VB denotes contains
T12170 32940-32941 DT denotes a
T12171 32942-32951 NN denotes substrate
T12172 32952-32955 IN denotes for
T12173 32956-32964 NN denotes alkaline
T12174 32965-32976 NN denotes phosphatase
T12175 32977-32980 CC denotes and
T12176 32981-32988 NN denotes changes
T12177 32989-32991 IN denotes in
T12178 32992-32995 DT denotes the
T12179 32996-33002 NN denotes amount
T12180 33003-33005 IN denotes of
T12181 33006-33016 NN denotes NF-κB/AP-1
T12182 33017-33025 NN denotes activity
T12183 33026-33030 VB denotes were
T12184 33031-33041 VB denotes quantified
T12185 33042-33044 IN denotes by
T12186 33045-33052 JJ denotes optical
T12187 33053-33060 NN denotes density
T12188 33061-33062 -LRB- denotes (
T12189 33062-33069 CD denotes λ = 655
T12190 33069-33070 -RRB- denotes )
T12191 33071-33079 VB denotes measured
T12192 33080-33085 VB denotes using
T12193 33086-33087 DT denotes a
T12194 33088-33098 NN denotes SpectraMax
T12195 33099-33102 CD denotes 384
T12196 33103-33107 NN denotes Plus
T12197 33108-33113 NN denotes plate
T12198 33114-33120 NN denotes reader
T12199 33121-33124 CC denotes and
T12200 33125-33132 NNP denotes SoftMax
T12201 33133-33136 NN denotes Pro
T12202 33137-33145 NN denotes software
T12203 33146-33147 -LRB- denotes (
T12204 33147-33156 NNP denotes Molecular
T12205 33157-33164 NNP denotes Devices
T12206 33164-33165 -RRB- denotes )
T12383 33637-33639 IN denotes of
T12304 33168-33177 JJ denotes Bacterial
T12305 33178-33185 NN denotes strains
T12306 33186-33188 NNP denotes P.
T12307 33189-33199 NN denotes aeruginosa
T12308 33200-33210 NN denotes laboratory
T12309 33211-33218 NN denotes strains
T12310 33219-33223 NN denotes PAO1
T12311 33224-33227 CC denotes and
T12312 33228-33231 DT denotes the
T12313 33232-33241 NN denotes PAO1ΔexsA
T12314 33242-33248 NN denotes mutant
T12315 33249-33253 VB denotes were
T12316 33254-33262 VB denotes obtained
T12317 33263-33267 IN denotes from
T12318 33268-33271 NNP denotes Dr.
T12319 33272-33278 NNP denotes Robert
T12320 33279-33287 NNP denotes Hancock.
T12321 33288-33290 NNP denotes P.
T12322 33291-33301 NN denotes aeruginosa
T12323 33302-33309 NN denotes strains
T12324 33310-33314 NN denotes PAO1
T12325 33315-33318 CC denotes and
T12326 33319-33328 NN denotes PAO1ΔexsA
T12327 33329-33333 VB denotes were
T12328 33334-33339 VB denotes grown
T12329 33340-33344 IN denotes from
T12330 33345-33354 JJ denotes overnight
T12331 33355-33363 NN denotes cultures
T12332 33364-33366 IN denotes in
T12333 33367-33372 NNP denotes Luria
T12334 33373-33380 NNP denotes Bertani
T12335 33381-33382 -LRB- denotes (
T12336 33382-33384 NN denotes LB
T12337 33384-33385 -RRB- denotes )
T12338 33386-33391 NN denotes broth
T12339 33392-33395 CC denotes and
T12340 33396-33411 NN denotes LB+streptomycin
T12341 33412-33413 -LRB- denotes (
T12342 33413-33416 CD denotes 150
T12343 33417-33422 NN denotes µg/ml
T12344 33422-33423 -RRB- denotes )
T12345 33424-33429 IN denotes until
T12346 33430-33445 JJ denotes mid-logarithmic
T12347 33446-33451 NN denotes phase
T12348 33453-33458 NN denotes Cells
T12349 33459-33463 VB denotes were
T12350 33464-33470 VB denotes washed
T12351 33471-33475 RB denotes once
T12352 33476-33478 IN denotes in
T12353 33479-33482 NNP denotes PBS
T12354 33483-33486 CC denotes and
T12355 33487-33498 VB denotes resuspended
T12356 33499-33501 IN denotes in
T12357 33502-33505 NNP denotes PBS
T12358 33506-33508 TO denotes to
T12359 33509-33511 DT denotes an
T12360 33512-33519 JJ denotes optical
T12361 33520-33527 NN denotes density
T12362 33528-33530 IN denotes of
T12363 33531-33534 CD denotes 0.5
T12364 33535-33536 -LRB- denotes (
T12365 33536-33543 CD denotes λ = 600
T12366 33544-33546 NN denotes nm
T12367 33546-33547 -RRB- denotes )
T12368 33549-33551 TO denotes To
T12369 33552-33559 VB denotes prepare
T12370 33560-33571 JJ denotes heat-killed
T12371 33572-33580 NN denotes bacteria
T12372 33580-33581 -COMMA- denotes ,
T12373 33582-33586 JJ denotes live
T12374 33587-33591 NN denotes PAO1
T12375 33592-33595 VB denotes was
T12376 33596-33607 VB denotes resuspended
T12377 33608-33610 IN denotes in
T12378 33611-33614 NNP denotes PBS
T12379 33615-33617 TO denotes to
T12380 33618-33620 DT denotes an
T12381 33621-33628 JJ denotes optical
T12382 33629-33636 NN denotes density
T12384 33640-33643 CD denotes 0.5
T12385 33644-33647 CC denotes and
T12386 33648-33654 VB denotes heated
T12387 33655-33657 IN denotes at
T12388 33658-33662 NN denotes 60°C
T12389 33663-33666 IN denotes for
T12390 33667-33668 CD denotes 1
T12391 33669-33673 NN denotes hour
T12392 33675-33678 IN denotes For
T12393 33679-33691 NN denotes stimulations
T12394 33691-33692 -COMMA- denotes ,
T12395 33693-33697 JJ denotes live
T12396 33698-33702 NN denotes PAO1
T12397 33703-33706 VB denotes was
T12398 33707-33718 VB denotes resuspended
T12399 33719-33721 TO denotes to
T12400 33722-33724 DT denotes an
T12401 33725-33732 JJ denotes optical
T12402 33733-33740 NN denotes density
T12403 33741-33743 IN denotes of
T12404 33744-33747 CD denotes 0.5
T12405 33748-33750 IN denotes in
T12406 33751-33754 NNP denotes PBS
T12407 33755-33758 CC denotes and
T12408 33759-33766 RB denotes further
T12409 33767-33774 VB denotes diluted
T12410 33775-33777 IN denotes in
T12411 33778-33785 NN denotes culture
T12412 33786-33792 NN denotes medium
T12413 33793-33798 JJ denotes prior
T12414 33799-33801 TO denotes to
T12415 33802-33813 NN denotes stimulation
T12416 33814-33816 TO denotes to
T12417 33817-33824 VB denotes achieve
T12418 33825-33828 DT denotes the
T12419 33829-33836 VB denotes desired
T12420 33837-33849 NN denotes multiplicity
T12421 33850-33852 IN denotes of
T12422 33853-33862 NN denotes infection
T12423 33864-33875 JJ denotes Heat-killed
T12424 33876-33880 NN denotes PAO1
T12425 33881-33884 VB denotes was
T12426 33885-33890 VB denotes added
T12427 33891-33893 IN denotes in
T12428 33894-33895 DT denotes a
T12429 33896-33902 NN denotes volume
T12430 33903-33913 JJ denotes equivalent
T12431 33914-33916 TO denotes to
T12432 33917-33921 DT denotes that
T12433 33922-33926 VB denotes used
T12434 33927-33929 TO denotes to
T12435 33930-33937 VB denotes achieve
T12436 33938-33940 DT denotes an
T12437 33941-33944 NN denotes MOI
T12438 33945-33947 IN denotes of
T12439 33948-33949 CD denotes 1
T12440 33950-33953 IN denotes for
T12441 33954-33958 JJ denotes live
T12442 33959-33963 NN denotes PAO1
T12609 33966-33976 NN denotes Statistics
T12610 33977-33980 DT denotes All
T12611 33981-33987 NN denotes graphs
T12612 33988-33995 VB denotes display
T12613 33996-33999 DT denotes the
T12614 34000-34004 JJ denotes mean
T12615 34005-34006 NN denotes ±
T12616 34007-34010 NN denotes SEM
T12617 34011-34014 CC denotes and
T12618 34015-34019 VB denotes were
T12619 34020-34029 VB denotes generated
T12620 34030-34034 IN denotes with
T12621 34035-34040 NN denotes Prism
T12622 34041-34042 CD denotes 5
T12623 34043-34044 -LRB- denotes (
T12624 34044-34052 NN denotes Graphpad
T12625 34052-34053 -RRB- denotes )
T12626 34055-34066 JJ denotes Statistical
T12627 34067-34079 NN denotes significance
T12628 34080-34083 VB denotes was
T12629 34084-34094 VB denotes determined
T12630 34095-34097 IN denotes by
T12631 34098-34108 VB denotes performing
T12632 34109-34112 CD denotes one
T12633 34113-34115 CC denotes or
T12634 34116-34123 JJ denotes two-way
T12635 34124-34129 NN denotes ANOVA
T12636 34130-34133 CC denotes and
T12637 34134-34137 DT denotes the
T12638 34138-34148 NNP denotes Bonferroni
T12639 34149-34158 JJ denotes post-test
T12640 34159-34164 WRB denotes where
T12641 34165-34175 JJ denotes applicable
T12683 6912-6916 NN denotes Cell
T12684 6917-6928 NN denotes stimulation
T12685 6929-6932 CC denotes and
T12686 6933-6942 NN denotes inhibitor
T12687 6943-6951 NN denotes schedule
T12688 6953-6961 NNP denotes Schedule
T12689 6962-6970 VB denotes outlines
T12690 6971-6974 DT denotes the
T12691 6975-6981 NN denotes timing
T12692 6982-6984 IN denotes of
T12693 6985-6994 NN denotes inhibitor
T12694 6995-7003 NN denotes addition
T12695 7004-7007 CC denotes and
T12696 7008-7015 NN denotes priming
T12697 7016-7018 IN denotes in
T12698 7019-7027 NN denotes relation
T12699 7028-7030 TO denotes to
T12700 7031-7043 JJ denotes inflammasome
T12701 7044-7055 NN denotes stimulation
T12702 7056-7057 -LRB- denotes (
T12703 7057-7062 NN denotes t = 0
T12704 7062-7063 -RRB- denotes )
T12705 7064-7067 IN denotes for
T12706 7068-7073 NN denotes THP-1
T12707 7074-7082 NN denotes reporter
T12708 7083-7086 CC denotes and
T12709 7087-7091 NN denotes PBMC
T12710 7092-7100 NN denotes cytokine
T12711 7101-7115 NN denotes quantification
T12712 7116-7127 NN denotes experiments
T12713 7129-7138 NN denotes Inhibitor
T12714 7139-7149 NN denotes treatments
T12715 7150-7153 CC denotes and
T12716 7154-7166 NN denotes stimulations
T12717 7167-7171 VB denotes were
T12718 7172-7179 VB denotes carried
T12719 7180-7183 RP denotes out
T12720 7184-7186 RB denotes as
T12721 7187-7196 VB denotes described
T12722 7197-7199 IN denotes in
T12723 7200-7203 DT denotes the
T12724 7204-7213 NNP denotes Materials
T12725 7214-7217 CC denotes and
T12726 7218-7225 NNP denotes Methods
T12727 7226-7233 NN denotes section
T12814 7279-7285 RB denotes Airway
T12815 7286-7296 JJ denotes epithelial
T12816 7297-7302 NN denotes cells
T12817 7303-7305 VB denotes do
T12818 7306-7309 RB denotes not
T12819 7310-7323 RB denotes significantly
T12820 7324-7334 VB denotes contribute
T12821 7335-7337 TO denotes to
T12822 7338-7343 NN denotes IL-1β
T12823 7344-7354 NN denotes production
T12824 7355-7357 IN denotes in
T12825 7358-7366 NN denotes response
T12826 7367-7369 TO denotes to
T12827 7370-7382 JJ denotes inflammasome
T12828 7383-7390 NN denotes stimuli
T12829 7392-7399 NN denotes Control
T12830 7400-7404 NN denotes cell
T12831 7405-7410 NN denotes lines
T12832 7411-7412 -LRB- denotes (
T12833 7412-7413 -LRB- denotes (
T12834 7413-7414 NN denotes A
T12835 7414-7415 -RRB- denotes )
T12836 7416-7418 NN denotes S9
T12837 7418-7419 -COMMA- denotes ,
T12838 7420-7421 -LRB- denotes (
T12839 7421-7422 NN denotes C
T12840 7422-7423 -RRB- denotes )
T12841 7424-7430 NN denotes NuLi-1
T12842 7430-7431 -RRB- denotes )
T12843 7432-7435 CC denotes and
T12844 7436-7441 PRP-DOLLAR- denotes their
T12845 7442-7455 VB denotes corresponding
T12846 7456-7458 NN denotes CF
T12847 7459-7463 NN denotes cell
T12848 7464-7469 NN denotes lines
T12849 7470-7471 -LRB- denotes (
T12850 7471-7472 -LRB- denotes (
T12851 7472-7473 NN denotes B
T12852 7473-7474 -RRB- denotes )
T12853 7475-7480 NN denotes IB3-1
T12854 7480-7481 -COMMA- denotes ,
T12855 7482-7485 CC denotes and
T12856 7486-7487 -LRB- denotes (
T12857 7487-7488 NN denotes D
T12858 7488-7489 -RRB- denotes )
T12859 7490-7496 NN denotes CuFi-1
T12860 7496-7497 -RRB- denotes )
T12861 7498-7503 NN denotes cells
T12862 7504-7508 VB denotes were
T12863 7509-7519 VB denotes stimulated
T12864 7520-7524 IN denotes with
T12865 7525-7527 NNP denotes P.
T12866 7528-7538 NN denotes aeruginosa
T12867 7539-7540 -LRB- denotes (
T12868 7540-7548 NN denotes MOI = 10
T12869 7548-7549 -RRB- denotes )
T12870 7549-7550 -COMMA- denotes ,
T12871 7551-7554 NN denotes ATP
T12872 7555-7556 -LRB- denotes (
T12873 7556-7557 CD denotes 5
T12874 7558-7560 NN denotes mM
T12875 7560-7561 -RRB- denotes )
T12876 7561-7562 -COMMA- denotes ,
T12877 7563-7565 CC denotes or
T12878 7566-7571 NN denotes IL-1β
T12879 7572-7573 -LRB- denotes (
T12880 7573-7575 CD denotes 10
T12881 7576-7581 NN denotes ng/ml
T12882 7581-7582 -RRB- denotes )
T12883 7582-7583 -COMMA- denotes ,
T12884 7584-7587 IN denotes for
T12885 7588-7591 DT denotes the
T12886 7592-7601 VB denotes indicated
T12887 7602-7607 NN denotes times
T12888 7608-7609 -LRB- denotes (
T12889 7609-7614 NN denotes n = 3
T12890 7615-7625 JJ denotes individual
T12891 7626-7637 NN denotes experiments
T12892 7637-7638 -RRB- denotes )
T12893 7640-7645 NN denotes Cells
T12894 7646-7650 VB denotes were
T12895 7651-7657 VB denotes primed
T12896 7658-7662 IN denotes with
T12897 7663-7666 NN denotes LPS
T12898 7667-7668 -LRB- denotes (
T12899 7668-7671 CD denotes 100
T12900 7672-7677 NN denotes ng/ml
T12901 7677-7678 -RRB- denotes )
T12902 7679-7682 IN denotes for
T12903 7683-7684 CD denotes 4
T12904 7685-7690 NN denotes hours
T12905 7691-7696 WRB denotes where
T12906 7697-7708 JJ denotes appropriate
T12907 7710-7714 NN denotes Cell
T12908 7715-7722 NN denotes culture
T12909 7723-7735 NN denotes supernatants
T12910 7736-7740 VB denotes were
T12911 7741-7748 VB denotes assayed
T12912 7749-7752 IN denotes for
T12913 7753-7758 NN denotes IL-1β
T12914 7759-7762 CC denotes and
T12915 7763-7767 NN denotes IL-8
T12916 7768-7778 NN denotes production
T12917 7779-7781 IN denotes by
T12918 7782-7787 NN denotes ELISA
T12919 7789-7795 NN denotes Insert
T12920 7796-7801 VB denotes shows
T12921 7802-7806 NN denotes IL-8
T12922 7807-7816 NN denotes secretion
T12923 7817-7819 IN denotes in
T12924 7820-7828 NN denotes response
T12925 7829-7831 TO denotes to
T12926 7832-7843 NN denotes stimulation
T12927 7844-7848 IN denotes with
T12928 7849-7854 NN denotes IL-1β
T12929 7855-7856 -LRB- denotes (
T12930 7856-7858 CD denotes 10
T12931 7859-7864 NN denotes ng/ml
T12932 7864-7865 -RRB- denotes )
T13121 8724-8730 NN denotes Airway
T13122 8731-8736 NN denotes cells
T13123 8737-8739 VB denotes do
T13124 8740-8743 RB denotes not
T13125 8744-8752 RB denotes strongly
T13126 8753-8763 VB denotes upregulate
T13127 8764-8773 NN denotes caspase-1
T13128 8774-8782 NN denotes activity
T13129 8783-8785 IN denotes in
T13130 8786-8794 NN denotes response
T13131 8795-8797 TO denotes to
T13132 8798-8810 JJ denotes inflammasome
T13133 8811-8818 NN denotes stimuli
T13134 8820-8822 NN denotes S9
T13135 8823-8826 CC denotes and
T13136 8827-8832 NN denotes IB3-1
T13137 8833-8838 NN denotes cells
T13138 8839-8843 VB denotes were
T13139 8844-8852 VB denotes examined
T13140 8853-8856 IN denotes for
T13141 8857-8866 NN denotes caspase-1
T13142 8867-8877 NN denotes activation
T13143 8878-8887 VB denotes following
T13144 8888-8900 JJ denotes inflammasome
T13145 8901-8912 NN denotes stimulation
T13146 8913-8917 IN denotes with
T13147 8918-8920 NNP denotes P.
T13148 8921-8931 NN denotes aeruginosa
T13149 8932-8933 -LRB- denotes (
T13150 8933-8941 NN denotes MOI = 10
T13151 8941-8942 -RRB- denotes )
T13152 8943-8946 CC denotes and
T13153 8947-8950 NN denotes ATP
T13154 8951-8952 -LRB- denotes (
T13155 8952-8953 CD denotes 5
T13156 8954-8956 NN denotes mM
T13157 8956-8957 -RRB- denotes )
T13158 8959-8964 NN denotes Cells
T13159 8965-8969 VB denotes were
T13160 8970-8976 VB denotes primed
T13161 8977-8981 IN denotes with
T13162 8982-8985 NN denotes LPS
T13163 8986-8989 IN denotes for
T13164 8990-8991 CD denotes 5
T13165 8992-8997 NN denotes hours
T13166 8998-9003 WRB denotes where
T13167 9004-9015 JJ denotes appropriate
T13168 9017-9018 DT denotes A
T13169 9019-9033 JJ denotes representative
T13170 9034-9043 NN denotes histogram
T13171 9044-9046 IN denotes of
T13172 9047-9048 NN denotes %
T13173 9049-9065 JJ denotes caspase-1-active
T13174 9066-9071 NN denotes cells
T13175 9072-9074 VB denotes is
T13176 9075-9080 VB denotes shown
T13177 9081-9083 IN denotes in
T13178 9084-9085 -LRB- denotes (
T13179 9085-9086 NN denotes A
T13180 9086-9087 -RRB- denotes )
T13181 9088-9091 CC denotes and
T13182 9092-9095 DT denotes the
T13183 9096-9104 JJ denotes averaged
T13184 9105-9111 NN denotes values
T13185 9112-9115 VB denotes are
T13186 9116-9121 VB denotes shown
T13187 9122-9124 IN denotes in
T13188 9125-9126 -LRB- denotes (
T13189 9126-9127 NN denotes B
T13190 9127-9128 -RRB- denotes )
T13191 9129-9130 -LRB- denotes (
T13192 9130-9135 NN denotes n = 3
T13193 9136-9144 JJ denotes separate
T13194 9145-9156 NN denotes experiments
T13195 9156-9157 -RRB- denotes )
T13196 9157-9158 -LRB- denotes .
T13197 9159-9160 NN denotes (
T13198 9160-9161 -RRB- denotes C
T13199 9161-9166 NN denotes ) IB3
T13200 9166-9171 NN denotes -1 ce
T13201 9171-9172 -LRB- denotes l
T13202 9172-9177 CD denotes ls (5
T13203 9177-9187 NN denotes ×104 cells
T13204 9187-9188 -RRB- denotes /
T13205 9188-9192 VB denotes well
T13206 9192-9203 VB denotes ) were pre-
T13207 9203-9206 IN denotes tre
T13208 9206-9207 CD denotes a
T13209 9207-9211 NN denotes ted
T13210 9211-9215 IN denotes for
T13211 9215-9225 VB denotes 1 hour wit
T13212 9225-9239 NN denotes h increasing c
T13213 9239-9241 IN denotes on
T13214 9241-9251 NN denotes centration
T13215 9251-9252 -LRB- denotes s
T13216 9253-9258 CD denotes of z-
T13217 9258-9260 NN denotes YV
T13218 9260-9261 -RRB- denotes A
T13219 9261-9266 JJ denotes D-fmk
T13220 9267-9269 TO denotes (1
T13221 9269-9280 NN denotes 0–30 µM) pr
T13222 9280-9284 IN denotes ior
T13223 9284-9286 NNP denotes to
T13224 9287-9297 NN denotes stimulatio
T13225 9297-9298 -LRB- denotes n
T13226 9299-9307 NN denotes with P.
T13227 9307-9308 -RRB- denotes a
T13228 9330-9334 NN denotes Cell
T13229 9335-9342 NN denotes culture
T13230 9343-9355 NN denotes supernatants
T13231 9356-9360 VB denotes were
T13232 9361-9370 VB denotes collected
T13233 9371-9376 IN denotes after
T13234 9377-9378 CD denotes 6
T13235 9379-9384 NN denotes hours
T13236 9385-9388 CC denotes and
T13237 9389-9396 VB denotes assayed
T13238 9397-9400 IN denotes for
T13239 9401-9405 NN denotes IL-6
T13240 9406-9408 IN denotes by
T13241 9409-9414 NN denotes ELISA
T13242 9415-9416 -LRB- denotes (
T13243 9416-9421 NN denotes n = 3
T13244 9421-9422 -RRB- denotes )
T13410 9944-9949 NN denotes PBMCs
T13411 9950-9954 IN denotes from
T13412 9955-9957 NN denotes CF
T13413 9958-9966 NN denotes patients
T13414 9967-9970 CC denotes and
T13415 9971-9979 NN denotes controls
T13416 9980-9984 VB denotes show
T13417 9985-9992 JJ denotes similar
T13418 9993-10002 NN denotes increases
T13419 10003-10005 IN denotes in
T13420 10006-10015 NN denotes caspase-1
T13421 10016-10024 NN denotes activity
T13422 10025-10029 IN denotes upon
T13423 10030-10042 JJ denotes inflammasome
T13424 10043-10053 NN denotes activation
T13425 10055-10060 NN denotes PBMCs
T13426 10061-10065 IN denotes from
T13427 10066-10068 NN denotes CF
T13428 10069-10077 NN denotes patients
T13429 10078-10079 -LRB- denotes (
T13430 10079-10084 NN denotes n = 6
T13431 10084-10085 -RRB- denotes )
T13432 10086-10089 CC denotes and
T13433 10090-10097 JJ denotes healthy
T13434 10098-10106 NN denotes controls
T13435 10107-10108 -LRB- denotes (
T13436 10108-10113 NN denotes n = 6
T13437 10113-10114 -RRB- denotes )
T13438 10115-10119 VB denotes were
T13439 10120-10126 VB denotes primed
T13440 10127-10131 IN denotes with
T13441 10132-10135 NN denotes LPS
T13442 10136-10137 -LRB- denotes (
T13443 10137-10139 CD denotes 10
T13444 10140-10145 NN denotes ng/ml
T13445 10145-10146 -RRB- denotes )
T13446 10147-10150 IN denotes for
T13447 10151-10152 CD denotes 5
T13448 10153-10158 NN denotes hours
T13449 10159-10164 RB denotes prior
T13450 10165-10167 TO denotes to
T13451 10168-10179 NN denotes stimulation
T13452 10180-10184 IN denotes with
T13453 10185-10188 NN denotes ATP
T13454 10189-10190 -LRB- denotes (
T13455 10190-10191 CD denotes 5
T13456 10192-10194 NN denotes mM
T13457 10194-10195 -RRB- denotes )
T13458 10196-10199 IN denotes for
T13459 10200-10201 CD denotes 1
T13460 10202-10206 NN denotes hour
T13461 10207-10209 CC denotes or
T13462 10210-10214 NN denotes Poly
T13463 10214-10215 -LRB- denotes (
T13464 10215-10217 NN denotes dA
T13465 10217-10218 -COLON- denotes :
T13466 10218-10220 NN denotes dT
T13467 10220-10221 -RRB- denotes )
T13468 10222-10223 -LRB- denotes (
T13469 10223-10224 CD denotes 1
T13470 10225-10230 NN denotes µg/ml
T13471 10230-10231 -RRB- denotes )
T13472 10232-10235 IN denotes for
T13473 10236-10237 CD denotes 3
T13474 10238-10243 NN denotes hours
T13475 10245-10250 NN denotes PBMCs
T13476 10251-10255 VB denotes were
T13477 10256-10266 VB denotes stimulated
T13478 10267-10271 IN denotes with
T13479 10272-10274 NNP denotes P.
T13480 10275-10285 NN denotes aeruginosa
T13481 10286-10292 NN denotes strain
T13482 10293-10297 NN denotes PAO1
T13483 10298-10301 IN denotes for
T13484 10302-10303 CD denotes 3
T13485 10304-10309 NN denotes hours
T13486 10311-10312 DT denotes A
T13487 10313-10327 JJ denotes representative
T13488 10328-10337 NN denotes histogram
T13489 10338-10340 IN denotes of
T13490 10341-10344 DT denotes the
T13491 10345-10346 NN denotes %
T13492 10347-10363 JJ denotes caspase-1-active
T13493 10364-10369 NN denotes cells
T13494 10370-10372 VB denotes is
T13495 10373-10378 VB denotes shown
T13496 10379-10381 IN denotes in
T13497 10382-10383 -LRB- denotes (
T13498 10383-10384 NN denotes A
T13499 10384-10385 -RRB- denotes )
T13500 10386-10390 IN denotes with
T13501 10391-10394 DT denotes the
T13502 10395-10403 JJ denotes averaged
T13503 10404-10410 NN denotes values
T13504 10411-10416 VB denotes shown
T13505 10417-10419 IN denotes in
T13506 10420-10421 -LRB- denotes (
T13507 10421-10422 NN denotes B
T13508 10422-10423 -RRB- denotes )
T13645 11893-11898 NN denotes PBMCs
T13646 11899-11903 IN denotes from
T13647 11904-11906 NN denotes CF
T13648 11907-11915 NN denotes patients
T13649 11916-11918 VB denotes do
T13650 11919-11922 RB denotes not
T13651 11923-11930 VB denotes produce
T13652 11931-11940 VB denotes increased
T13653 11941-11948 NN denotes amounts
T13654 11949-11951 IN denotes of
T13655 11952-11957 NN denotes IL-1β
T13656 11959-11964 NN denotes PBMCs
T13657 11965-11969 IN denotes from
T13658 11970-11972 NN denotes CF
T13659 11973-11981 NN denotes patients
T13660 11982-11983 -LRB- denotes (
T13661 11983-11992 NN denotes n = 17–20
T13662 11992-11993 -RRB- denotes )
T13663 11994-11997 CC denotes and
T13664 11998-12005 JJ denotes healthy
T13665 12006-12014 NN denotes controls
T13666 12015-12016 -LRB- denotes (
T13667 12016-12025 NN denotes n = 15–19
T13668 12025-12026 -RRB- denotes )
T13669 12027-12031 VB denotes were
T13670 12032-12038 VB denotes primed
T13671 12039-12043 IN denotes with
T13672 12044-12047 NN denotes LPS
T13673 12048-12049 -LRB- denotes (
T13674 12049-12051 CD denotes 10
T13675 12052-12057 NN denotes ng/ml
T13676 12057-12058 -RRB- denotes )
T13677 12059-12068 JJ denotes overnight
T13678 12069-12072 CC denotes and
T13679 12073-12083 VB denotes stimulated
T13680 12084-12088 IN denotes with
T13681 12089-12091 NNP denotes P.
T13682 12092-12102 NN denotes aeruginosa
T13683 12103-12107 NN denotes PAO1
T13684 12108-12109 -LRB- denotes (
T13685 12109-12116 NN denotes MOI = 1
T13686 12116-12117 -RRB- denotes )
T13687 12117-12118 -COMMA- denotes ,
T13688 12119-12121 NNP denotes P.
T13689 12122-12132 NN denotes aeruginosa
T13690 12133-12137 NN denotes PAO1
T13691 12138-12145 VB denotes lacking
T13692 12146-12150 NN denotes exsA
T13693 12151-12152 -LRB- denotes (
T13694 12152-12159 NN denotes MOI = 1
T13695 12159-12160 -RRB- denotes )
T13696 12160-12161 -COMMA- denotes ,
T13697 12162-12165 NN denotes ATP
T13698 12166-12167 -LRB- denotes (
T13699 12167-12168 CD denotes 5
T13700 12169-12171 NN denotes mM
T13701 12171-12172 -RRB- denotes )
T13702 12172-12173 -COMMA- denotes ,
T13703 12174-12176 CC denotes or
T13704 12177-12181 NNP denotes Poly
T13705 12181-12182 -LRB- denotes (
T13706 12182-12184 NN denotes dA
T13707 12184-12185 -COLON- denotes :
T13708 12185-12187 NN denotes dT
T13709 12187-12188 -RRB- denotes )
T13710 12189-12190 -LRB- denotes (
T13711 12190-12191 CD denotes 1
T13712 12192-12197 NN denotes µg/ml
T13713 12197-12198 -RRB- denotes )
T13714 12199-12202 IN denotes for
T13715 12203-12205 CD denotes 24
T13716 12206-12211 NN denotes hours
T13717 12213-12215 NN denotes P.
T13718 12216-12226 NN denotes aeruginosa
T13719 12227-12234 VB denotes lacking
T13720 12235-12239 NN denotes exsA
T13721 12240-12243 VB denotes was
T13722 12244-12248 VB denotes used
T13723 12249-12251 IN denotes as
T13724 12252-12253 DT denotes a
T13725 12254-12258 NN denotes type
T13726 12259-12262 CD denotes III
T13727 12263-12272 NN denotes secretion
T13728 12273-12280 NN denotes control
T13729 12281-12283 IN denotes in
T13730 12284-12294 NN denotes comparison
T13731 12295-12299 IN denotes with
T13732 12300-12309 JJ denotes wild-type
T13733 12310-12312 NNP denotes P.
T13734 12313-12323 NN denotes aeruginosa
T13735 12325-12337 NN denotes Supernatants
T13736 12338-12342 VB denotes were
T13737 12343-12350 VB denotes assayed
T13738 12351-12354 IN denotes for
T13739 12355-12356 -LRB- denotes (
T13740 12356-12357 NN denotes A
T13741 12357-12358 -RRB- denotes )
T13742 12359-12364 NN denotes IL-1β
T13743 12365-12368 CC denotes and
T13744 12369-12370 -LRB- denotes (
T13745 12370-12371 NN denotes B
T13746 12371-12372 -RRB- denotes )
T13747 12373-12377 NN denotes IL-8
T13942 14091-14096 NN denotes NF-κB
T13943 14097-14107 NN denotes activation
T13944 14108-14119 VB denotes potentiates
T13945 14120-14123 DT denotes the
T13946 14124-14130 NN denotes degree
T13947 14131-14133 IN denotes of
T13948 14134-14139 NN denotes IL-1β
T13949 14140-14150 NN denotes production
T13950 14151-14154 CC denotes and
T13951 14155-14164 NN denotes secretion
T13952 14165-14169 IN denotes upon
T13953 14170-14182 JJ denotes inflammasome
T13954 14183-14193 NN denotes activation
T13955 14195-14200 NN denotes THP-1
T13956 14201-14209 NN denotes reporter
T13957 14210-14215 NN denotes cells
T13958 14216-14220 VB denotes were
T13959 14221-14227 VB denotes primed
T13960 14228-14237 RB denotes overnight
T13961 14238-14242 IN denotes with
T13962 14243-14254 JJ denotes heat-killed
T13963 14255-14257 NNP denotes P.
T13964 14258-14268 NN denotes aeruginosa
T13965 14269-14272 CC denotes and
T13966 14273-14283 VB denotes stimulated
T13967 14284-14287 DT denotes the
T13968 14288-14292 JJ denotes next
T13969 14293-14296 NN denotes day
T13970 14297-14301 IN denotes with
T13971 14302-14306 JJ denotes live
T13972 14307-14309 NNP denotes P.
T13973 14310-14320 NN denotes aeruginosa
T13974 14321-14323 CC denotes or
T13975 14324-14334 JJ denotes additional
T13976 14335-14346 JJ denotes heat-killed
T13977 14347-14349 NNP denotes P.
T13978 14350-14360 NN denotes aeruginosa
T13979 14361-14364 IN denotes for
T13980 14365-14368 DT denotes the
T13981 14369-14374 NN denotes times
T13982 14375-14384 VB denotes indicated
T13983 14386-14390 NN denotes Cell
T13984 14391-14398 NN denotes culture
T13985 14399-14411 NN denotes supernatants
T13986 14412-14416 VB denotes were
T13987 14417-14424 VB denotes assayed
T13988 14425-14428 IN denotes for
T13989 14429-14430 -LRB- denotes (
T13990 14430-14431 NN denotes A
T13991 14431-14432 -RRB- denotes )
T13992 14433-14443 NN denotes NF-κB/AP-1
T13993 14444-14452 NN denotes activity
T13994 14452-14453 -COMMA- denotes ,
T13995 14454-14455 -LRB- denotes (
T13996 14455-14456 NN denotes B
T13997 14456-14457 -RRB- denotes )
T13998 14458-14462 NN denotes IL-8
T13999 14462-14463 -COMMA- denotes ,
T14000 14464-14467 CC denotes and
T14001 14468-14469 -LRB- denotes (
T14002 14469-14470 NN denotes C
T14003 14470-14471 -RRB- denotes )
T14004 14472-14477 NN denotes IL-1β
T14005 14478-14487 NN denotes secretion
T14006 14488-14489 -LRB- denotes (
T14007 14489-14496 NN denotes n = 3–6
T14008 14497-14508 NN denotes experiments
T14009 14508-14509 -RRB- denotes )
T14010 14511-14516 VB denotes Using
T14011 14517-14520 DT denotes the
T14012 14521-14525 JJ denotes same
T14013 14526-14537 NN denotes stimulation
T14014 14538-14544 NN denotes method
T14015 14544-14545 -COMMA- denotes ,
T14016 14546-14551 NN denotes THP-1
T14017 14552-14560 NN denotes reporter
T14018 14561-14566 NN denotes cells
T14019 14567-14571 VB denotes were
T14020 14572-14579 VB denotes treated
T14021 14580-14584 IN denotes with
T14022 14585-14595 NN denotes Bay11-7082
T14023 14596-14597 -LRB- denotes (
T14024 14597-14599 CD denotes 20
T14025 14600-14602 NN denotes µM
T14026 14602-14603 -RRB- denotes )
T14027 14604-14607 IN denotes for
T14028 14608-14609 CD denotes 1
T14029 14610-14614 NN denotes hour
T14030 14615-14620 JJ denotes prior
T14031 14621-14623 TO denotes to
T14032 14624-14631 NN denotes priming
T14033 14632-14636 IN denotes with
T14034 14637-14648 JJ denotes heat-killed
T14035 14649-14653 NN denotes PAO1
T14036 14654-14656 CC denotes or
T14037 14657-14661 JJ denotes live
T14038 14662-14666 NN denotes PAO1
T14039 14668-14680 NN denotes Supernatants
T14040 14681-14685 VB denotes were
T14041 14686-14693 VB denotes assayed
T14042 14694-14696 IN denotes at
T14043 14697-14699 CD denotes 24
T14044 14700-14705 NN denotes hours
T14045 14706-14709 IN denotes for
T14046 14710-14711 -LRB- denotes (
T14047 14711-14712 NN denotes D
T14048 14712-14713 -RRB- denotes )
T14049 14714-14724 NN denotes NF-κB/AP-1
T14050 14725-14733 NN denotes activity
T14051 14733-14734 -COMMA- denotes ,
T14052 14735-14736 -LRB- denotes (
T14053 14736-14737 NN denotes E
T14054 14737-14738 -RRB- denotes )
T14055 14739-14743 NN denotes IL-8
T14056 14743-14744 -COMMA- denotes ,
T14057 14745-14748 CC denotes and
T14058 14749-14750 -LRB- denotes (
T14059 14750-14751 NN denotes F
T14060 14751-14752 -RRB- denotes )
T14061 14753-14758 NN denotes IL-1β
T14062 14759-14768 NN denotes secretion
T14063 14769-14770 -LRB- denotes (
T14064 14770-14777 NN denotes n = 3–5
T14065 14777-14778 -RRB- denotes )
T14066 14780-14785 NN denotes PBMCs
T14067 14786-14790 IN denotes from
T14068 14791-14793 NN denotes CF
T14069 14794-14802 NN denotes patients
T14070 14803-14804 -LRB- denotes (
T14071 14804-14813 NN denotes n = 11–15
T14072 14813-14814 -RRB- denotes )
T14073 14815-14818 CC denotes and
T14074 14819-14827 NN denotes controls
T14075 14828-14829 -LRB- denotes (
T14076 14829-14838 NN denotes n = 10–13
T14077 14838-14839 -RRB- denotes )
T14078 14840-14844 VB denotes were
T14079 14845-14852 VB denotes treated
T14080 14853-14857 IN denotes with
T14081 14858-14868 NN denotes z-YVAD-fmk
T14082 14869-14870 -LRB- denotes (
T14083 14870-14872 CD denotes 20
T14084 14873-14875 NN denotes µM
T14085 14875-14876 -RRB- denotes )
T14086 14877-14879 CC denotes or
T14087 14880-14890 NN denotes Bay11-7082
T14088 14891-14892 -LRB- denotes (
T14089 14892-14894 CD denotes 10
T14090 14895-14897 NN denotes µM
T14091 14897-14898 -RRB- denotes )
T14092 14899-14902 CC denotes and
T14093 14903-14913 VB denotes stimulated
T14094 14914-14918 IN denotes with
T14095 14919-14923 JJ denotes live
T14096 14924-14928 NN denotes PAO1
T14097 14929-14930 -LRB- denotes (
T14098 14930-14937 NN denotes MOI = 1
T14099 14937-14938 -RRB- denotes )
T14100 14938-14939 -COMMA- denotes ,
T14101 14940-14943 NN denotes ATP
T14102 14944-14945 -LRB- denotes (
T14103 14945-14946 CD denotes 5
T14104 14947-14949 NN denotes mM
T14105 14949-14950 -RRB- denotes )
T14106 14950-14951 -COMMA- denotes ,
T14107 14952-14954 CC denotes or
T14108 14955-14959 NNP denotes Poly
T14109 14959-14960 -LRB- denotes (
T14110 14960-14962 NN denotes dA
T14111 14962-14963 -COLON- denotes :
T14112 14963-14965 NN denotes dT
T14113 14965-14966 -RRB- denotes )
T14114 14967-14968 -LRB- denotes (
T14115 14968-14969 CD denotes 1
T14116 14970-14975 NN denotes µg/ml
T14117 14975-14976 -RRB- denotes )
T14118 14977-14986 VB denotes according
T14119 14987-14989 TO denotes to
T14120 14990-14993 DT denotes the
T14121 14994-15002 NN denotes schedule
T14122 15003-15005 IN denotes in
T14123 15006-15012 NN denotes Figure
T14124 15013-15015 CD denotes 1.
T14125 15016-15017 -LRB- denotes (
T14126 15017-15018 NN denotes G
T14127 15018-15019 -RRB- denotes )
T14128 15020-15025 NN denotes IL-1β
T14129 15026-15029 CC denotes and
T14130 15030-15031 -LRB- denotes (
T14131 15031-15032 NN denotes H
T14132 15032-15033 -RRB- denotes )
T14133 15034-15038 NN denotes IL-8
T14134 15039-15045 NN denotes levels
T14135 15046-15050 VB denotes were
T14136 15051-15059 VB denotes measured
T14137 15060-15062 IN denotes at
T14138 15063-15065 CD denotes 24
T14139 15066-15071 NN denotes hours
T14140 15073-15084 JJ denotes Statistical
T14141 15085-15093 NN denotes analysis
T14142 15094-15097 VB denotes was
T14143 15098-15107 VB denotes performed
T14144 15108-15113 VB denotes using
T14145 15114-15117 CD denotes two
T14146 15118-15121 NN denotes way
T14147 15122-15127 NN denotes ANOVA
T14148 15128-15132 IN denotes with
T14149 15133-15143 NNP denotes Bonferroni
T14150 15144-15154 NN denotes correction
T14151 15155-15158 IN denotes for
T14152 15159-15167 JJ denotes multiple
T14153 15168-15180 NN denotes comparisons.
T14154 15181-15182 SYM denotes *
T14155 15182-15183 -COMMA- denotes ,
T14156 15184-15186 SYM denotes **
T14157 15186-15187 -COMMA- denotes ,
T14158 15188-15191 CC denotes and
T14159 15192-15195 SYM denotes ***
T14160 15196-15203 VB denotes signify
T14161 15204-15205 NN denotes P
T14162 15205-15206 SYM denotes <
T14163 15206-15210 CD denotes 0.05
T14164 15210-15211 -COMMA- denotes ,
T14165 15212-15216 CD denotes 0.01
T14166 15216-15217 -COMMA- denotes ,
T14167 15218-15221 CC denotes and
T14168 15222-15227 CD denotes 0.001
T14505 15927-15937 NN denotes Bay11-7082
T14506 15938-15946 VB denotes inhibits
T14507 15947-15956 JJ denotes pro-IL-1β
T14508 15957-15967 NN denotes production
T14509 15968-15970 IN denotes in
T14510 15971-15979 NN denotes response
T14511 15980-15982 TO denotes to
T14512 15983-15985 NNP denotes P.
T14513 15986-15996 NN denotes aeruginosa
T14514 15998-16016 JJ denotes PMA-differentiated
T14515 16017-16022 NN denotes THP-1
T14516 16023-16028 NN denotes cells
T14517 16029-16033 VB denotes were
T14518 16034-16041 VB denotes treated
T14519 16042-16046 IN denotes with
T14520 16047-16049 CD denotes 10
T14521 16050-16052 NN denotes µM
T14522 16053-16063 NN denotes CFTRinh172
T14523 16064-16066 CC denotes or
T14524 16067-16069 CD denotes 20
T14525 16070-16072 NN denotes µM
T14526 16073-16083 NN denotes Bay11-7082
T14527 16084-16087 CC denotes and
T14528 16088-16097 VB denotes harvested
T14529 16098-16103 IN denotes after
T14530 16104-16105 -LRB- denotes (
T14531 16105-16106 NN denotes A
T14532 16106-16107 -RRB- denotes )
T14533 16108-16109 CD denotes 4
T14534 16110-16115 NN denotes hours
T14535 16116-16117 -LRB- denotes (
T14536 16117-16122 NN denotes n = 3
T14537 16122-16123 -RRB- denotes )
T14538 16124-16126 CC denotes or
T14539 16127-16128 -LRB- denotes (
T14540 16128-16129 NN denotes B
T14541 16129-16130 -RRB- denotes )
T14542 16131-16134 CD denotes 0.5
T14543 16134-16135 -COMMA- denotes ,
T14544 16136-16137 CD denotes 1
T14545 16137-16138 -COMMA- denotes ,
T14546 16139-16142 CC denotes and
T14547 16143-16146 CD denotes 1.5
T14548 16147-16152 NN denotes hours
T14549 16153-16154 -LRB- denotes (
T14550 16154-16159 NN denotes n = 3
T14551 16159-16160 -RRB- denotes )
T14552 16161-16172 NN denotes stimulation
T14553 16173-16177 IN denotes with
T14554 16178-16182 NN denotes PAO1
T14555 16184-16187 CD denotes One
T14556 16188-16202 NN denotes representative
T14557 16203-16207 NN denotes blot
T14558 16208-16210 VB denotes is
T14559 16211-16216 VB denotes shown
T14560 16217-16221 IN denotes with
T14561 16222-16223 DT denotes a
T14562 16224-16229 NN denotes graph
T14563 16230-16232 IN denotes of
T14564 16233-16236 DT denotes the
T14565 16237-16245 JJ denotes averaged
T14566 16246-16258 NN denotes fluorescence
T14567 16259-16268 NN denotes intensity
T14568 16269-16275 NN denotes values
T14569 16276-16280 IN denotes over
T14570 16281-16282 CD denotes 3
T14571 16283-16294 NN denotes experiments
T14739 17437-17445 NN denotes cytes fr
T14674 17130-17140 NN denotes Disruption
T14675 17141-17143 IN denotes of
T14676 17144-17148 NN denotes CFTR
T14677 17149-17157 NN denotes activity
T14678 17158-17162 VB denotes does
T14679 17163-17166 RB denotes not
T14680 17167-17175 VB denotes increase
T14681 17176-17181 NN denotes IL-1β
T14682 17182-17192 NN denotes production
T14683 17193-17195 IN denotes in
T14684 17196-17201 NN denotes PBMCs
T14685 17202-17204 CC denotes or
T14686 17205-17216 NN denotes macrophages
T14687 17218-17223 NN denotes PBMCs
T14688 17224-17228 IN denotes from
T14689 17229-17231 NN denotes CF
T14690 17232-17240 NN denotes patients
T14691 17241-17242 -LRB- denotes (
T14692 17242-17248 NN denotes n = 15
T14693 17248-17249 -RRB- denotes )
T14694 17250-17253 CC denotes and
T14695 17254-17262 NN denotes controls
T14696 17263-17264 -LRB- denotes (
T14697 17264-17270 NN denotes n = 13
T14698 17270-17271 -RRB- denotes )
T14699 17272-17276 VB denotes were
T14700 17277-17284 VB denotes treated
T14701 17285-17289 IN denotes with
T14702 17290-17300 NN denotes CFTRinh172
T14703 17301-17302 -LRB- denotes (
T14704 17302-17304 CD denotes 10
T14705 17305-17307 NN denotes µM
T14706 17307-17308 -RRB- denotes )
T14707 17309-17312 IN denotes for
T14708 17313-17315 CD denotes 18
T14709 17316-17321 NN denotes hours
T14710 17322-17327 RB denotes prior
T14711 17328-17330 TO denotes to
T14712 17331-17342 NN denotes stimulation
T14713 17343-17347 IN denotes with
T14714 17348-17352 JJ denotes live
T14715 17353-17357 NN denotes PAO1
T14716 17358-17359 -LRB- denotes (
T14717 17359-17366 NN denotes MOI = 1
T14718 17366-17367 -RRB- denotes )
T14719 17367-17368 -LRB- denotes .
T14720 17369-17370 NN denotes (
T14721 17370-17371 -RRB- denotes A
T14722 17371-17376 NN denotes ) IL-
T14723 17376-17379 CC denotes 1β
T14724 17379-17380 -LRB- denotes a
T14725 17380-17381 NN denotes n
T14726 17381-17382 -RRB- denotes d
T14727 17383-17387 NN denotes (B)
T14728 17387-17397 NN denotes IL-8 produ
T14729 17397-17400 VB denotes cti
T14730 17400-17408 VB denotes on was m
T14731 17408-17410 IN denotes ea
T14732 17410-17412 CD denotes su
T14733 17412-17418 NN denotes red at
T14734 17419-17420 -LRB- denotes 2
T14735 17420-17421 NN denotes 4
T14736 17422-17423 -RRB- denotes h
T14737 17423-17432 NN denotes ours. (C)
T14738 17433-17437 IN denotes Mono
T14740 17445-17446 -LRB- denotes o
T14741 17446-17451 NN denotes m con
T14742 17451-17452 -RRB- denotes t
T14743 17452-17456 VB denotes rols
T14744 17457-17471 VB denotes (n = 3) were d
T14745 17471-17475 IN denotes iffe
T14746 17475-17486 NN denotes rentiated i
T14747 17503-17514 NN denotes Macrophages
T14748 17515-17519 VB denotes were
T14749 17520-17527 VB denotes treated
T14750 17528-17532 IN denotes with
T14751 17533-17543 NN denotes CFTRinh172
T14752 17543-17544 -COMMA- denotes ,
T14753 17545-17555 VB denotes stimulated
T14754 17556-17558 IN denotes as
T14755 17559-17562 IN denotes per
T14756 17563-17572 NN denotes monocytes
T14757 17572-17573 -COMMA- denotes ,
T14758 17574-17577 CC denotes and
T14759 17578-17586 VB denotes measured
T14760 17587-17590 IN denotes for
T14761 17591-17596 NN denotes IL-1β
T14762 17597-17607 NN denotes production
T14763 17608-17610 IN denotes at
T14764 17611-17613 CD denotes 24
T14765 17614-17619 NN denotes hours
T14766 17621-17626 NN denotes THP-1
T14767 17627-17635 NN denotes reporter
T14768 17636-17641 NN denotes cells
T14769 17642-17646 VB denotes were
T14770 17647-17654 VB denotes treated
T14771 17655-17659 IN denotes with
T14772 17660-17670 NN denotes CFTRinh172
T14773 17671-17673 CD denotes 24
T14774 17674-17679 NN denotes hours
T14775 17680-17685 RB denotes prior
T14776 17686-17688 TO denotes to
T14777 17689-17700 NN denotes stimulation
T14778 17701-17705 IN denotes with
T14779 17706-17710 NN denotes PAO1
T14780 17711-17714 CC denotes and
T14781 17715-17723 VB denotes measured
T14782 17724-17727 IN denotes for
T14783 17728-17729 -LRB- denotes (
T14784 17729-17730 NN denotes D
T14785 17730-17731 -RRB- denotes )
T14786 17732-17737 NN denotes IL-1β
T14787 17738-17747 NN denotes secretion
T14788 17747-17748 -COMMA- denotes ,
T14789 17749-17750 -LRB- denotes (
T14790 17750-17751 NN denotes E
T14791 17751-17752 -RRB- denotes )
T14792 17753-17757 NN denotes IL-8
T14793 17757-17758 -COMMA- denotes ,
T14794 17759-17762 CC denotes and
T14795 17763-17764 -LRB- denotes (
T14796 17764-17765 NN denotes F
T14797 17765-17766 -RRB- denotes )
T14798 17767-17777 NN denotes NF-κB/AP-1
T14799 17778-17786 NN denotes activity
T14800 17787-17789 IN denotes at
T14801 17790-17792 CD denotes 24
T14802 17793-17798 NN denotes hours
T14803 17799-17800 -LRB- denotes (
T14804 17800-17805 NN denotes n = 4
T14805 17805-17806 -RRB- denotes )
T15014 7910-7922 JJ denotes Inflammasome
T15015 7923-7933 NN denotes Activators
T6195 18548-18556 JJ denotes alveolar
R45 T91 T89 arg1Of Production,Inflammasome-Mediated
R46 T91 T90 arg1Of Production,IL-1β
R47 T91 T92 arg1Of Production,in
R48 T93 T92 arg2Of Humans,in
R49 T93 T94 arg1Of Humans,with
R50 T96 T94 arg2Of Fibrosis,with
R51 T96 T95 arg1Of Fibrosis,Cystic
R52 T98 T99 arg1Of Inflammation,and
R53 T99 T101 arg1Of and,are
R54 T100 T99 arg2Of infection,and
R55 T103 T101 arg2Of determinants,are
R56 T103 T102 arg1Of determinants,major
R57 T103 T104 arg1Of determinants,of
R58 T103 T108 arg1Of determinants,consequently
R59 T103 T109 arg1Of determinants,","
R60 T104 T107 arg1Of of,and
R61 T106 T104 arg2Of severity,of
R62 T106 T105 arg1Of severity,disease
R63 T108 T107 arg2Of consequently,and
R64 T111 T109 arg2Of quality,","
R65 T111 T110 arg1Of quality,the
R66 T111 T112 arg1Of quality,of
R67 T111 T116 arg1Of quality,for
R68 T113 T114 arg1Of life,and
R69 T114 T112 arg2Of and,of
R70 T115 T114 arg2Of outcome,and
R71 T117 T116 arg2Of patients,for
R72 T117 T118 arg1Of patients,with
R73 T120 T118 arg2Of fibrosis,with
R74 T120 T119 arg1Of fibrosis,cystic
R75 T120 T121 arg1Of fibrosis,(
R76 T122 T121 arg2Of CF,(
R77 T123 T121 arg3Of ),(
R78 T125 T124 arg1Of beta,Interleukin-1
R79 T125 T126 arg1Of beta,(
R80 T125 T129 arg1Of beta,is
R81 T127 T126 arg2Of IL-1β,(
R82 T128 T126 arg3Of ),(
R83 T133 T129 arg2Of mediator,is
R84 T133 T130 arg1Of mediator,a
R85 T133 T131 arg1Of mediator,key
R86 T133 T132 arg1Of mediator,inflammatory
R87 T134 T135 arg1Of Secretion,of
R88 T134 T139 arg1Of Secretion,involves
R89 T137 T136 arg1Of active,biologically
R90 T138 T135 arg2Of IL-1β,of
R91 T138 T137 arg1Of IL-1β,active
R92 T141 T139 arg2Of processing,involves
R93 T141 T140 arg1Of processing,inflammasome-mediated
R94 T142 T143 arg1Of Little,is
R95 T142 T144 arg2Of Little,known
R96 T144 T143 arg2Of known,is
R97 T144 T145 arg1Of known,about
R98 T147 T145 arg2Of contribution,about
R99 T147 T146 arg1Of contribution,the
R100 T147 T148 arg1Of contribution,of
R101 T147 T153 arg1Of contribution,in
R102 T149 T150 arg1Of IL-1β,and
R103 T150 T148 arg2Of and,of
R104 T152 T150 arg2Of inflammasomes,and
R105 T152 T151 arg1Of inflammasomes,the
R106 T156 T153 arg2Of disease,in
R107 T156 T154 arg1Of disease,CF
R108 T156 T155 arg1Of disease,inflammatory
R109 T158 T157 arg1Of study,This
R110 T158 T159 arg1Of study,examines
R111 T162 T159 arg2Of production,examines
R112 T162 T160 arg1Of production,inflammasome-mediated
R113 T162 T161 arg1Of production,IL-1β
R114 T162 T163 arg1Of production,in
R115 T168 T164 arg1Of lines,CF
R116 T168 T165 arg1Of lines,bronchial
R117 T168 T166 arg1Of lines,epithelial
R118 T168 T167 arg1Of lines,cell
R119 T168 T169 arg1Of lines,and
R120 T169 T163 arg2Of and,in
R121 T171 T169 arg2Of patients,and
R122 T171 T170 arg1Of patients,human
R123 T171 T172 arg1Of patients,with
R124 T173 T172 arg2Of CF,with
R125 T178 T175 arg1Of lines,Bronchial
R126 T178 T176 arg1Of lines,epithelial
R127 T178 T177 arg1Of lines,cell
R128 T178 T179 arg1Of lines,were
R129 T178 T180 arg2Of lines,found
R130 T178 T182 arg1Of lines,produce
R131 T180 T179 arg2Of found,were
R132 T180 T190 arg1Of found,compared
R133 T180 T194 arg1Of found,and
R134 T182 T180 arg3Of produce,found
R135 T182 T181 arg1Of produce,to
R136 T184 T182 arg2Of amounts,produce
R137 T184 T183 arg1Of amounts,negligible
R138 T184 T185 arg1Of amounts,of
R139 T186 T187 arg1Of basal,or
R140 T186 T188 arg2Of basal,stimulated
R141 T189 T185 arg2Of IL-1β,of
R142 T189 T186 arg1Of IL-1β,basal
R143 T191 T190 arg2Of to,compared
R144 T193 T191 arg2Of cells,to
R145 T193 T192 arg1Of cells,hematopoeitic
R146 T195 T196 arg1Of they,did
R147 T195 T199 arg1Of they,upregulate
R148 T199 T194 arg2Of upregulate,and
R149 T199 T196 arg2Of upregulate,did
R150 T199 T197 arg1Of upregulate,not
R151 T199 T198 arg1Of upregulate,significantly
R152 T199 T202 arg1Of upregulate,upon
R153 T201 T199 arg2Of activity,upregulate
R154 T201 T200 arg1Of activity,caspase-1
R155 T204 T202 arg2Of stimulation,upon
R156 T204 T203 arg1Of stimulation,inflammasome
R157 T206 T205 arg2Of contrast,In
R158 T211 T208 arg1Of cells,peripheral
R159 T211 T209 arg1Of cells,blood
R160 T211 T210 arg1Of cells,mononuclear
R161 T211 T212 arg1Of cells,(
R162 T211 T215 arg1Of cells,from
R163 T211 T222 arg1Of cells,produced
R164 T213 T212 arg2Of PBMCs,(
R165 T214 T212 arg3Of ),(
R166 T221 T215 arg2Of subjects,from
R167 T221 T216 arg1Of subjects,both
R168 T221 T217 arg1Of subjects,CF
R169 T221 T218 arg1Of subjects,and
R170 T221 T219 arg1Of subjects,healthy
R171 T221 T220 arg1Of subjects,control
R172 T222 T205 arg1Of produced,In
R173 T222 T207 arg1Of produced,","
R174 T224 T222 arg2Of amounts,produced
R175 T224 T223 arg1Of amounts,large
R176 T224 T225 arg1Of amounts,of
R177 T226 T227 arg1Of IL-1β,and
R178 T227 T225 arg2Of and,of
R179 T227 T232 arg1Of and,upon
R180 T229 T228 arg1Of upregulated,strongly
R181 T231 T227 arg2Of activity,and
R182 T231 T229 arg2Of activity,upregulated
R183 T231 T230 arg1Of activity,caspase-1
R184 T234 T232 arg2Of stimulation,upon
R185 T234 T233 arg1Of stimulation,inflammasome
R186 T235 T236 arg1Of PBMCs,from
R187 T235 T241 arg1Of PBMCs,displayed
R188 T238 T237 arg1Of patients,CF
R189 T238 T239 arg1Of patients,and
R190 T239 T236 arg2Of and,from
R191 T240 T239 arg2Of controls,and
R192 T241 T250 arg1Of displayed,when
R193 T243 T241 arg2Of levels,displayed
R194 T243 T242 arg1Of levels,similar
R195 T243 T244 arg1Of levels,of
R196 T246 T245 arg1Of activation,caspase-1
R197 T246 T247 arg1Of activation,and
R198 T247 T244 arg2Of and,of
R199 T249 T247 arg2Of production,and
R200 T249 T248 arg1Of production,IL-1β
R201 T251 T250 arg2Of stimulated,when
R202 T251 T252 arg1Of stimulated,with
R203 T254 T252 arg2Of activators,with
R204 T254 T253 arg1Of activators,inflammasome
R205 T257 T255 arg1Of production,This
R206 T257 T256 arg1Of production,IL-1β
R207 T257 T258 arg1Of production,was
R208 T257 T259 arg1Of production,dependent
R209 T257 T264 arg1Of production,could
R210 T257 T265 arg1Of production,be
R211 T257 T266 arg2Of production,enhanced
R212 T258 T263 arg1Of was,and
R213 T259 T258 arg2Of dependent,was
R214 T259 T260 arg1Of dependent,on
R215 T262 T260 arg2Of activity,on
R216 T262 T261 arg1Of activity,NF-κB
R217 T266 T263 arg2Of enhanced,and
R218 T266 T264 arg2Of enhanced,could
R219 T266 T265 arg2Of enhanced,be
R220 T266 T267 arg1Of enhanced,by
R221 T268 T267 arg2Of priming,by
R222 T268 T269 arg1Of priming,with
R223 T270 T269 arg2Of LPS,with
R224 T274 T273 arg1Of inhibition,chemical
R225 T274 T275 arg1Of inhibition,of
R226 T274 T278 arg1Of inhibition,in
R227 T274 T284 arg1Of inhibition,did
R228 T274 T287 arg1Of inhibition,alter
R229 T277 T275 arg2Of activity,of
R230 T277 T276 arg1Of activity,CFTR
R231 T280 T279 arg1Of PBMCs,control
R232 T280 T281 arg1Of PBMCs,and
R233 T281 T278 arg2Of and,in
R234 T283 T281 arg2Of cells,and
R235 T283 T282 arg1Of cells,THP-1
R236 T287 T271 arg1Of alter,Finally
R237 T287 T272 arg1Of alter,","
R238 T287 T284 arg2Of alter,did
R239 T287 T285 arg1Of alter,not
R240 T287 T286 arg1Of alter,significantly
R241 T287 T292 arg1Of alter,in
R242 T288 T289 arg1Of IL-1β,or
R243 T290 T289 arg2Of IL-8,or
R244 T291 T287 arg2Of production,alter
R245 T291 T288 arg1Of production,IL-1β
R246 T291 T290 arg1Of production,IL-8
R247 T293 T292 arg2Of response,in
R248 T293 T294 arg1Of response,to
R249 T296 T294 arg2Of aeruginosa,to
R250 T296 T295 arg1Of aeruginosa,P.
R251 T299 T298 arg1Of cells,Hematopoeitic
R252 T299 T300 arg1Of cells,appear
R253 T299 T302 arg1Of cells,be
R254 T302 T300 arg2Of be,appear
R255 T302 T301 arg1Of be,to
R256 T305 T302 arg2Of source,be
R257 T305 T303 arg1Of source,the
R258 T305 T304 arg1Of source,predominant
R259 T305 T306 arg1Of source,of
R260 T305 T310 arg1Of source,in
R261 T309 T306 arg2Of IL-1β,of
R262 T309 T307 arg1Of IL-1β,inflammasome-induced
R263 T309 T308 arg1Of IL-1β,pro-inflammatory
R264 T311 T310 arg2Of CF,in
R265 T312 T313 arg2Of PBMCs,derived
R266 T312 T317 arg1Of PBMCs,display
R267 T313 T314 arg1Of derived,from
R268 T316 T314 arg2Of subjects,from
R269 T316 T315 arg1Of subjects,CF
R270 T317 T324 arg1Of display,in
R271 T320 T318 arg2Of activation,preserved
R272 T320 T319 arg1Of activation,inflammasome
R273 T320 T321 arg1Of activation,and
R274 T321 T317 arg2Of and,display
R275 T323 T321 arg2Of secretion,and
R276 T323 T322 arg1Of secretion,IL-1β
R277 T324 T326 arg1Of in,to
R278 T325 T324 arg2Of response,in
R279 T332 T324 arg3Of aeruginosa,in
R280 T332 T327 arg1Of aeruginosa,the
R281 T332 T328 arg1Of aeruginosa,major
R282 T332 T329 arg1Of aeruginosa,CF
R283 T332 T330 arg1Of aeruginosa,pathogen
R284 T332 T331 arg1Of aeruginosa,Pseudomonas
R285 T336 T335 arg1Of data,our
R286 T336 T337 arg1Of data,do
R287 T336 T339 arg1Of data,support
R288 T339 T333 arg1Of support,However
R289 T339 T334 arg1Of support,","
R290 T339 T337 arg2Of support,do
R291 T339 T338 arg1Of support,not
R292 T341 T339 arg2Of hypothesis,support
R293 T341 T340 arg1Of hypothesis,the
R294 T345 T343 arg2Of production,increased
R295 T345 T344 arg1Of production,IL-1β
R296 T345 T346 arg1Of production,in
R297 T345 T349 arg1Of production,is
R298 T345 T351 arg1Of production,to
R299 T348 T346 arg2Of subjects,in
R300 T348 T347 arg1Of subjects,CF
R301 T349 T341 arg2Of is,hypothesis
R302 T349 T342 arg1Of is,that
R303 T351 T349 arg2Of to,is
R304 T351 T350 arg1Of to,due
R305 T354 T351 arg2Of increase,to
R306 T354 T352 arg1Of increase,an
R307 T354 T353 arg1Of increase,intrinsic
R308 T354 T355 arg1Of increase,in
R309 T357 T355 arg2Of activity,in
R310 T357 T356 arg1Of activity,NF-κB
R311 T357 T358 arg1Of activity,through
R312 T359 T358 arg2Of loss,through
R313 T359 T360 arg1Of loss,of
R314 T362 T360 arg2Of function,of
R315 T362 T361 arg1Of function,CFTR
R736 T953 T952 arg1Of fibrosis,Cystic
R737 T953 T954 arg1Of fibrosis,is
R738 T958 T954 arg2Of disease,is
R739 T958 T955 arg1Of disease,an
R740 T958 T956 arg1Of disease,autosomal
R741 T958 T957 arg1Of disease,recessive
R742 T958 T959 arg2Of disease,caused
R743 T961 T959 arg1Of mutations,caused
R744 T961 T960 arg2Of mutations,by
R745 T961 T962 arg1Of mutations,in
R746 T968 T962 arg2Of regulator,in
R747 T968 T963 arg1Of regulator,the
R748 T968 T964 arg1Of regulator,cystic
R749 T968 T965 arg1Of regulator,fibrosis
R750 T968 T966 arg1Of regulator,transmembrane
R751 T968 T967 arg1Of regulator,conductance
R752 T968 T969 arg1Of regulator,(
R753 T968 T972 arg1Of regulator,","
R754 T968 T973 arg1Of regulator,which
R755 T968 T974 arg1Of regulator,functions
R756 T970 T969 arg2Of CFTR,(
R757 T971 T969 arg3Of ),(
R758 T974 T975 arg1Of functions,as
R759 T979 T975 arg2Of channel,as
R760 T979 T976 arg1Of channel,a
R761 T979 T977 arg1Of channel,chloride
R762 T979 T978 arg1Of channel,ion
R763 T980 T981 arg1Of CF,remains
R764 T981 T1008 arg1Of remains,[
R765 T981 T1011 arg1Of remains,","
R766 T981 T1012 arg1Of remains,[
R767 T982 T981 arg2Of one,remains
R768 T982 T983 arg1Of one,of
R769 T982 T990 arg1Of one,in
R770 T986 T985 arg1Of common,most
R771 T989 T983 arg2Of diseases,of
R772 T989 T984 arg1Of diseases,the
R773 T989 T986 arg1Of diseases,common
R774 T989 T987 arg1Of diseases,lethal
R775 T989 T988 arg1Of diseases,genetic
R776 T991 T990 arg2Of populations,in
R777 T991 T992 arg1Of populations,of
R778 T991 T995 arg1Of populations,with
R779 T991 T1003 arg1Of populations,approximately
R780 T994 T992 arg2Of descent,of
R781 T994 T993 arg1Of descent,European
R782 T999 T995 arg2Of lifespan,with
R783 T999 T996 arg1Of lifespan,the
R784 T999 T997 arg1Of lifespan,current
R785 T999 T998 arg1Of lifespan,average
R786 T999 T1000 arg1Of lifespan,of
R787 T1002 T1000 arg2Of patients,of
R788 T1002 T1001 arg1Of patients,CF
R789 T1003 T1005 arg1Of approximately,years
R790 T1005 T1004 arg1Of years,40
R791 T1005 T1006 arg1Of years,of
R792 T1007 T1006 arg2Of age,of
R793 T1009 T1008 arg2Of 1,[
R794 T1010 T1008 arg3Of ],[
R795 T1013 T1012 arg2Of 2,[
R796 T1014 T1012 arg3Of ],[
R797 T1018 T1015 arg1Of exacerbation,Recurrent
R798 T1018 T1016 arg1Of exacerbation,inflammatory
R799 T1018 T1017 arg1Of exacerbation,pulmonary
R800 T1018 T1019 arg1Of exacerbation,is
R801 T1022 T1021 arg1Of cause,primary
R802 T1022 T1023 arg1Of cause,of
R803 T1022 T1028 arg1Of cause,ultimately
R804 T1022 T1029 arg1Of cause,","
R805 T1023 T1027 arg1Of of,and
R806 T1026 T1023 arg2Of progression,of
R807 T1026 T1024 arg1Of progression,lung
R808 T1026 T1025 arg1Of progression,disease
R809 T1028 T1027 arg2Of ultimately,and
R810 T1029 T1019 arg2Of ",",is
R811 T1029 T1020 arg1Of ",",the
R812 T1029 T1031 arg1Of ",",in
R813 T1030 T1029 arg2Of death,","
R814 T1032 T1031 arg2Of CF,in
R815 T1034 T1033 arg2Of inflammation,Controlled
R816 T1034 T1035 arg1Of inflammation,is
R817 T1034 T1036 arg1Of inflammation,important
R818 T1035 T1041 arg1Of is,but
R819 T1036 T1035 arg2Of important,is
R820 T1036 T1037 arg1Of important,for
R821 T1038 T1037 arg2Of fighting,for
R822 T1039 T1038 arg2Of infection,fighting
R823 T1041 T1040 arg1Of but,","
R824 T1043 T1042 arg2Of excess,in
R825 T1045 T1046 arg1Of it,becomes
R826 T1045 T1047 arg1Of it,destructive
R827 T1046 T1041 arg2Of becomes,but
R828 T1046 T1042 arg1Of becomes,in
R829 T1046 T1044 arg1Of becomes,","
R830 T1046 T1061 arg1Of becomes,","
R831 T1046 T1062 arg1Of becomes,[
R832 T1047 T1046 arg2Of destructive,becomes
R833 T1047 T1048 arg1Of destructive,to
R834 T1047 T1052 arg1Of destructive,to
R835 T1048 T1051 arg1Of to,and
R836 T1050 T1048 arg2Of cells,to
R837 T1050 T1049 arg1Of cells,host
R838 T1052 T1051 arg2Of to,and
R839 T1054 T1052 arg2Of architecture,to
R840 T1054 T1053 arg1Of architecture,the
R841 T1054 T1055 arg1Of architecture,of
R842 T1057 T1055 arg2Of lungs,of
R843 T1057 T1056 arg1Of lungs,the
R844 T1057 T1058 arg1Of lungs,[
R845 T1059 T1058 arg2Of 2,[
R846 T1060 T1058 arg3Of ],[
R847 T1063 T1062 arg2Of 3,[
R848 T1064 T1062 arg3Of ],[
R849 T1066 T1065 arg2Of CF,In
R850 T1070 T1068 arg1Of cells,airway
R851 T1070 T1069 arg1Of cells,epithelial
R852 T1070 T1071 arg1Of cells,have
R853 T1070 T1072 arg1Of cells,been
R854 T1070 T1073 arg2Of cells,shown
R855 T1070 T1075 arg1Of cells,produce
R856 T1073 T1065 arg1Of shown,In
R857 T1073 T1067 arg1Of shown,","
R858 T1073 T1071 arg2Of shown,have
R859 T1073 T1072 arg2Of shown,been
R860 T1075 T1073 arg3Of produce,shown
R861 T1075 T1074 arg1Of produce,to
R862 T1075 T1086 arg1Of produce,","
R863 T1075 T1087 arg1Of produce,[
R864 T1080 T1075 arg2Of response,produce
R865 T1080 T1076 arg1Of response,an
R866 T1080 T1077 arg1Of response,exaggerated
R867 T1080 T1078 arg1Of response,pro-inflammatory
R868 T1080 T1079 arg1Of response,cytokine
R869 T1080 T1081 arg1Of response,to
R870 T1082 T1081 arg2Of stimulation,to
R871 T1082 T1083 arg1Of stimulation,[
R872 T1084 T1083 arg2Of 4,[
R873 T1085 T1083 arg3Of ],[
R874 T1088 T1087 arg2Of 5,[
R875 T1089 T1087 arg3Of ],[
R876 T1092 T1091 arg2Of unclear,is
R877 T1097 T1094 arg1Of response,this
R878 T1097 T1095 arg2Of response,heightened
R879 T1097 T1096 arg1Of response,inflammatory
R880 T1097 T1098 arg1Of response,is
R881 T1097 T1099 arg1Of response,intrinsic
R882 T1098 T1093 arg1Of is,whether
R883 T1098 T1104 arg1Of is,or
R884 T1099 T1098 arg2Of intrinsic,is
R885 T1099 T1100 arg1Of intrinsic,to
R886 T1101 T1100 arg2Of cells,to
R887 T1101 T1102 arg1Of cells,lacking
R888 T1103 T1102 arg2Of CFTR,lacking
R889 T1104 T1090 arg1Of or,It
R890 T1104 T1091 arg1Of or,is
R891 T1104 T1092 arg1Of or,unclear
R892 T1105 T1104 arg2Of whether,or
R893 T1106 T1107 arg1Of it,is
R894 T1107 T1105 arg2Of is,whether
R895 T1109 T1107 arg2Of result,is
R896 T1109 T1108 arg1Of result,a
R897 T1109 T1110 arg1Of result,of
R898 T1113 T1110 arg2Of infection,of
R899 T1113 T1111 arg1Of infection,chronic
R900 T1113 T1112 arg1Of infection,polymicrobial
R901 T1113 T1114 arg1Of infection,[
R902 T1113 T1117 arg1Of infection,","
R903 T1113 T1118 arg1Of infection,[
R904 T1115 T1114 arg2Of 6,[
R905 T1116 T1114 arg3Of ],[
R906 T1119 T1118 arg2Of 7,[
R907 T1120 T1118 arg3Of ],[
R908 T1121 T1122 arg1Of Regardless,of
R909 T1124 T1122 arg2Of controversy,of
R910 T1124 T1123 arg1Of controversy,this
R911 T1126 T1127 arg1Of identifying,and
R912 T1127 T1121 arg1Of and,Regardless
R913 T1127 T1125 arg1Of and,","
R914 T1127 T1132 arg2Of and,is
R915 T1128 T1127 arg2Of targeting,and
R916 T1131 T1126 arg2Of mediators,identifying
R917 T1131 T1128 arg2Of mediators,targeting
R918 T1131 T1129 arg1Of mediators,relevant
R919 T1131 T1130 arg1Of mediators,inflammatory
R920 T1135 T1126 arg1Of step,identifying
R921 T1135 T1128 arg1Of step,targeting
R922 T1135 T1132 arg1Of step,is
R923 T1135 T1133 arg1Of step,a
R924 T1135 T1134 arg1Of step,critical
R925 T1135 T1136 arg1Of step,in
R926 T1137 T1136 arg2Of developing,in
R927 T1137 T1142 modOf developing,to
R928 T1137 T1149 arg1Of developing,in
R929 T1137 T1151 arg1Of developing,[
R930 T1140 T1139 arg1Of therapeutic,specific
R931 T1141 T1137 arg2Of approaches,developing
R932 T1141 T1138 arg1Of approaches,more
R933 T1141 T1140 arg1Of approaches,therapeutic
R934 T1143 T1145 arg1Of control,and
R935 T1144 T1143 arg2Of inflammation,control
R936 T1145 T1142 arg1Of and,to
R937 T1146 T1145 arg2Of improve,and
R938 T1148 T1146 arg2Of outcomes,improve
R939 T1148 T1147 arg1Of outcomes,health
R940 T1150 T1149 arg2Of CF,in
R941 T1152 T1151 arg2Of 8,[
R942 T1153 T1151 arg3Of ],[
R943 T1155 T1154 arg1Of beta,Interleukin-1
R944 T1155 T1156 arg1Of beta,(
R945 T1155 T1159 arg1Of beta,is
R946 T1157 T1156 arg2Of IL-1β,(
R947 T1158 T1156 arg3Of ),(
R948 T1163 T1159 arg2Of mediator,is
R949 T1163 T1160 arg1Of mediator,a
R950 T1163 T1161 arg1Of mediator,major
R951 T1163 T1162 arg1Of mediator,inflammatory
R952 T1166 T1164 arg1Of effects,Its
R953 T1166 T1165 arg1Of effects,physiological
R954 T1166 T1167 arg1Of effects,are
R955 T1166 T1168 arg1Of effects,diverse
R956 T1166 T1171 arg1Of effects,important
R957 T1168 T1169 arg1Of diverse,and
R958 T1169 T1167 arg2Of and,are
R959 T1171 T1169 arg2Of important,and
R960 T1171 T1170 arg1Of important,potentially
R961 T1171 T1172 arg1Of important,to
R962 T1174 T1172 arg2Of pathogenesis,to
R963 T1174 T1173 arg1Of pathogenesis,the
R964 T1174 T1175 arg1Of pathogenesis,of
R965 T1177 T1175 arg2Of exacerbations,of
R966 T1177 T1176 arg1Of exacerbations,lung
R967 T1177 T1178 arg1Of exacerbations,in
R968 T1177 T1180 arg1Of exacerbations,","
R969 T1177 T1181 arg1Of exacerbations,including
R970 T1179 T1178 arg2Of CF,in
R971 T1183 T1182 arg1Of generation,the
R972 T1183 T1184 arg1Of generation,of
R973 T1183 T1186 arg1Of generation,","
R974 T1183 T1193 arg1Of generation,","
R975 T1185 T1184 arg2Of fever,of
R976 T1188 T1186 arg2Of recruitment,","
R977 T1188 T1187 arg1Of recruitment,the
R978 T1188 T1189 arg1Of recruitment,of
R979 T1192 T1189 arg2Of cells,of
R980 T1192 T1190 arg1Of cells,inflammatory
R981 T1192 T1191 arg1Of cells,effector
R982 T1193 T1206 arg1Of ",",and
R983 T1195 T1193 arg2Of induction,","
R984 T1195 T1194 arg1Of induction,the
R985 T1195 T1196 arg1Of induction,of
R986 T1199 T1196 arg2Of cytokines,of
R987 T1199 T1197 arg1Of cytokines,other
R988 T1199 T1198 arg1Of cytokines,pro-inflammatory
R989 T1199 T1201 arg1Of cytokines,as
R990 T1201 T1200 arg1Of as,such
R991 T1202 T1203 arg1Of IL-6,and
R992 T1203 T1201 arg2Of and,as
R993 T1204 T1203 arg2Of IL-8,and
R994 T1206 T1181 arg2Of and,including
R995 T1206 T1205 arg1Of and,","
R996 T1208 T1206 arg2Of shaping,and
R997 T1208 T1207 arg1Of shaping,the
R998 T1208 T1209 arg1Of shaping,of
R999 T1212 T1209 arg2Of responses,of
R1000 T1212 T1210 arg1Of responses,T
R1001 T1212 T1211 arg1Of responses,cell
R1002 T1212 T1213 arg1Of responses,[
R1003 T1212 T1216 arg1Of responses,","
R1004 T1212 T1217 arg1Of responses,[
R1005 T1214 T1213 arg2Of 9,[
R1006 T1215 T1213 arg3Of ],[
R1007 T1218 T1217 arg2Of 10,[
R1008 T1219 T1217 arg3Of ],[
R1009 T1221 T1220 arg2Of initiation,Following
R1010 T1221 T1222 arg1Of initiation,of
R1011 T1226 T1222 arg2Of cascade,of
R1012 T1226 T1223 arg1Of cascade,the
R1013 T1226 T1224 arg1Of cascade,NF-κB
R1014 T1226 T1225 arg1Of cascade,signaling
R1015 T1228 T1229 arg1Of IL-1β,is
R1016 T1228 T1230 arg2Of IL-1β,produced
R1017 T1230 T1220 arg1Of produced,Following
R1018 T1230 T1227 arg1Of produced,","
R1019 T1230 T1229 arg2Of produced,is
R1020 T1230 T1231 arg1Of produced,in
R1021 T1230 T1234 arg1Of produced,as
R1022 T1233 T1231 arg2Of cytosol,in
R1023 T1233 T1232 arg1Of cytosol,the
R1024 T1239 T1234 arg2Of pro-IL-1β,as
R1025 T1239 T1235 arg1Of pro-IL-1β,a
R1026 T1239 T1236 arg1Of pro-IL-1β,biologically
R1027 T1239 T1237 arg1Of pro-IL-1β,inactive
R1028 T1239 T1238 arg1Of pro-IL-1β,full-length
R1029 T1240 T1241 arg1Of Pro-IL-1β,is
R1030 T1240 T1243 arg2Of Pro-IL-1β,converted
R1031 T1240 T1264 arg1Of Pro-IL-1β,mediate
R1032 T1243 T1241 arg2Of converted,is
R1033 T1243 T1242 arg1Of converted,subsequently
R1034 T1243 T1244 arg1Of converted,into
R1035 T1243 T1263 arg1Of converted,and
R1036 T1247 T1244 arg2Of form,into
R1037 T1247 T1245 arg1Of form,its
R1038 T1247 T1246 arg1Of form,active
R1039 T1251 T1243 arg1Of complexes,converted
R1040 T1251 T1248 arg2Of complexes,by
R1041 T1251 T1249 arg1Of complexes,cytosolic
R1042 T1251 T1250 arg1Of complexes,protein
R1043 T1251 T1252 arg2Of complexes,termed
R1044 T1252 T1256 arg1Of termed,in
R1045 T1255 T1252 arg3Of assemble,termed
R1046 T1255 T1253 arg1Of assemble,“inflammasomes.”
R1047 T1255 T1254 arg1Of assemble,Inflammasomes
R1048 T1257 T1256 arg2Of response,in
R1049 T1257 T1258 arg1Of response,to
R1050 T1262 T1258 arg2Of signals,to
R1051 T1262 T1259 arg1Of signals,certain
R1052 T1262 T1260 arg1Of signals,cellular
R1053 T1262 T1261 arg1Of signals,danger
R1054 T1264 T1263 arg2Of mediate,and
R1055 T1266 T1264 arg2Of auto-activation,mediate
R1056 T1266 T1265 arg1Of auto-activation,the
R1057 T1266 T1267 arg1Of auto-activation,of
R1058 T1266 T1273 arg1Of auto-activation,[
R1059 T1266 T1276 arg1Of auto-activation,","
R1060 T1266 T1277 arg1Of auto-activation,which
R1061 T1266 T1278 arg1Of auto-activation,cleaves
R1062 T1268 T1267 arg2Of caspase-1,of
R1063 T1268 T1269 arg1Of caspase-1,[
R1064 T1268 T1272 arg1Of caspase-1,","
R1065 T1270 T1269 arg2Of 9,[
R1066 T1271 T1269 arg3Of ],[
R1067 T1274 T1273 arg2Of 11,[
R1068 T1275 T1273 arg3Of ],[
R1069 T1279 T1280 arg1Of pro-IL-1β,and
R1070 T1280 T1278 arg2Of and,cleaves
R1071 T1280 T1282 arg1Of and,into
R1072 T1281 T1280 arg2Of pro-IL-18,and
R1073 T1285 T1284 arg1Of active,biologically
R1074 T1286 T1282 arg2Of forms,into
R1075 T1286 T1283 arg1Of forms,their
R1076 T1286 T1285 arg1Of forms,active
R1077 T1286 T1287 arg1Of forms,for
R1078 T1288 T1287 arg2Of secretion,for
R1079 T1291 T1289 arg1Of inflammasomes,Four
R1080 T1291 T1290 arg1Of inflammasomes,distinct
R1081 T1291 T1292 arg1Of inflammasomes,have
R1082 T1291 T1293 arg1Of inflammasomes,been
R1083 T1291 T1294 arg2Of inflammasomes,recognized
R1084 T1294 T1292 arg2Of recognized,have
R1085 T1294 T1293 arg2Of recognized,been
R1086 T1295 T1296 arg1Of These,are
R1087 T1298 T1299 arg1Of NLRP1,[
R1088 T1298 T1302 arg1Of NLRP1,","
R1089 T1300 T1299 arg2Of 12,[
R1090 T1301 T1299 arg3Of ],[
R1091 T1302 T1297 arg1Of ",",the
R1092 T1302 T1307 arg1Of ",",","
R1093 T1302 T1308 arg1Of ",",[
R1094 T1302 T1311 arg1Of ",",","
R1095 T1303 T1302 arg2Of NLRP3,","
R1096 T1303 T1304 arg1Of NLRP3,[
R1097 T1305 T1304 arg2Of 13,[
R1098 T1306 T1304 arg3Of ],[
R1099 T1309 T1308 arg2Of 14,[
R1100 T1310 T1308 arg3Of ],[
R1101 T1311 T1317 arg1Of ",",[
R1102 T1311 T1321 arg1Of ",",and
R1103 T1312 T1311 arg2Of NLRC4,","
R1104 T1312 T1313 arg1Of NLRC4,[
R1105 T1312 T1316 arg1Of NLRC4,","
R1106 T1314 T1313 arg2Of 15,[
R1107 T1315 T1313 arg3Of ],[
R1108 T1318 T1317 arg2Of 16,[
R1109 T1319 T1317 arg3Of ],[
R1110 T1321 T1296 arg2Of and,are
R1111 T1321 T1320 arg1Of and,","
R1112 T1323 T1321 arg2Of inflammasomes,and
R1113 T1323 T1322 arg1Of inflammasomes,AIM2
R1114 T1323 T1324 arg1Of inflammasomes,[
R1115 T1323 T1327 arg1Of inflammasomes,","
R1116 T1323 T1328 arg1Of inflammasomes,[
R1117 T1323 T1331 arg1Of inflammasomes,","
R1118 T1323 T1332 arg1Of inflammasomes,which
R1119 T1323 T1333 arg1Of inflammasomes,respond
R1120 T1325 T1324 arg2Of 17,[
R1121 T1326 T1324 arg3Of ],[
R1122 T1329 T1328 arg2Of 18,[
R1123 T1330 T1328 arg3Of ],[
R1124 T1333 T1334 arg1Of respond,to
R1125 T1336 T1334 arg2Of variety,to
R1126 T1336 T1335 arg1Of variety,a
R1127 T1336 T1337 arg1Of variety,of
R1128 T1340 T1338 arg1Of signatures,different
R1129 T1340 T1339 arg1Of signatures,microbial
R1130 T1340 T1341 arg1Of signatures,and
R1131 T1341 T1337 arg2Of and,of
R1132 T1343 T1341 arg2Of signals,and
R1133 T1343 T1342 arg1Of signals,danger
R1134 T1343 T1344 arg1Of signals,[
R1135 T1345 T1344 arg2Of 11,[
R1136 T1346 T1344 arg3Of ],[
R1137 T1348 T1347 arg1Of aeruginosa,P.
R1138 T1348 T1349 arg1Of aeruginosa,","
R1139 T1348 T1363 arg1Of aeruginosa,activates
R1140 T1350 T1349 arg2Of one,","
R1141 T1350 T1351 arg1Of one,of
R1142 T1350 T1359 arg1Of one,among
R1143 T1354 T1353 arg1Of common,most
R1144 T1354 T1355 arg1Of common,and
R1145 T1357 T1355 arg2Of relevant,and
R1146 T1357 T1356 arg1Of relevant,clinically
R1147 T1358 T1351 arg2Of pathogens,of
R1148 T1358 T1352 arg1Of pathogens,the
R1149 T1358 T1354 arg1Of pathogens,common
R1150 T1358 T1357 arg1Of pathogens,relevant
R1151 T1361 T1359 arg2Of patients,among
R1152 T1361 T1360 arg1Of patients,CF
R1153 T1363 T1362 arg1Of activates,","
R1154 T1363 T1370 arg1Of activates,","
R1155 T1363 T1371 arg1Of activates,[
R1156 T1366 T1363 arg2Of inflammasome,activates
R1157 T1366 T1364 arg1Of inflammasome,the
R1158 T1366 T1365 arg1Of inflammasome,NLRC4
R1159 T1366 T1367 arg1Of inflammasome,[
R1160 T1368 T1367 arg2Of 19,[
R1161 T1369 T1367 arg3Of ],[
R1162 T1372 T1371 arg2Of 20,[
R1163 T1373 T1371 arg3Of ],[
R1164 T1374 T1375 arg1Of Infection,with
R1165 T1374 T1378 arg1Of Infection,triggers
R1166 T1377 T1375 arg2Of aeruginosa,with
R1167 T1377 T1376 arg1Of aeruginosa,P.
R1168 T1378 T1401 arg1Of triggers,[
R1169 T1380 T1378 arg2Of increase,triggers
R1170 T1380 T1379 arg1Of increase,an
R1171 T1380 T1381 arg1Of increase,in
R1172 T1380 T1390 arg1Of increase,in
R1173 T1382 T1381 arg2Of levels,in
R1174 T1382 T1383 arg1Of levels,of
R1175 T1384 T1385 arg1Of IL-1β,","
R1176 T1385 T1388 arg1Of ",",and
R1177 T1386 T1385 arg2Of IL-6,","
R1178 T1388 T1383 arg2Of and,of
R1179 T1388 T1387 arg1Of and,","
R1180 T1389 T1388 arg2Of IL-8,and
R1181 T1393 T1390 arg2Of fluid,in
R1182 T1393 T1391 arg1Of fluid,bronchoalveolar
R1183 T1393 T1392 arg1Of fluid,lavage
R1184 T1393 T1394 arg1Of fluid,(
R1185 T1393 T1397 arg1Of fluid,from
R1186 T1395 T1394 arg2Of BALF,(
R1187 T1396 T1394 arg3Of ),(
R1188 T1398 T1397 arg2Of patients,from
R1189 T1398 T1399 arg1Of patients,with
R1190 T1400 T1399 arg2Of CF,with
R1191 T1402 T1401 arg2Of 21,[
R1192 T1403 T1401 arg3Of ],[
R1193 T1405 T1404 arg1Of responses,Inflammasome
R1194 T1405 T1406 arg1Of responses,depend
R1195 T1406 T1407 arg1Of depend,on
R1196 T1406 T1436 arg1Of depend,[
R1197 T1406 T1439 arg1Of depend,","
R1198 T1406 T1440 arg1Of depend,[
R1199 T1409 T1408 arg1Of signaling,NF-κB
R1200 T1409 T1410 arg1Of signaling,","
R1201 T1409 T1411 arg1Of signaling,where
R1202 T1409 T1426 arg1Of signaling,","
R1203 T1409 T1427 arg1Of signaling,[
R1204 T1409 T1433 arg1Of signaling,as
R1205 T1412 T1413 arg1Of NF-κB,is
R1206 T1412 T1414 arg1Of NF-κB,important
R1207 T1413 T1411 arg2Of is,where
R1208 T1414 T1413 arg2Of important,is
R1209 T1414 T1415 arg1Of important,in
R1210 T1418 T1415 arg2Of upregulation,in
R1211 T1418 T1416 arg1Of upregulation,both
R1212 T1418 T1417 arg1Of upregulation,the
R1213 T1418 T1419 arg1Of upregulation,of
R1214 T1422 T1419 arg2Of components,of
R1215 T1422 T1420 arg1Of components,specific
R1216 T1422 T1421 arg1Of components,inflammasome
R1217 T1422 T1423 arg1Of components,[
R1218 T1424 T1423 arg2Of 22,[
R1219 T1425 T1423 arg3Of ],[
R1220 T1428 T1427 arg2Of 23,[
R1221 T1429 T1427 arg3Of ],[
R1222 T1433 T1407 arg2Of as,on
R1223 T1433 T1430 arg1Of as,","
R1224 T1433 T1431 arg1Of as,as
R1225 T1433 T1432 arg1Of as,well
R1226 T1435 T1433 arg2Of expression,as
R1227 T1435 T1434 arg1Of expression,IL-1β
R1228 T1437 T1436 arg2Of 24,[
R1229 T1438 T1436 arg3Of ],[
R1230 T1441 T1440 arg2Of 25,[
R1231 T1442 T1440 arg3Of ],[
R1232 T1444 T1443 arg1Of studies,Previous
R1233 T1444 T1445 arg1Of studies,support
R1234 T1447 T1445 arg2Of role,support
R1235 T1447 T1446 arg1Of role,a
R1236 T1447 T1448 arg1Of role,for
R1237 T1447 T1450 arg1Of role,in
R1238 T1449 T1448 arg2Of IL-1β,for
R1239 T1452 T1450 arg2Of pathogenesis,in
R1240 T1452 T1451 arg1Of pathogenesis,the
R1241 T1452 T1453 arg1Of pathogenesis,of
R1242 T1457 T1453 arg2Of disease,of
R1243 T1457 T1454 arg1Of disease,CF
R1244 T1457 T1455 arg1Of disease,inflammatory
R1245 T1457 T1456 arg1Of disease,lung
R1246 T1458 T1459 arg1Of Levels,of
R1247 T1458 T1461 arg1Of Levels,are
R1248 T1458 T1462 arg2Of Levels,increased
R1249 T1460 T1459 arg2Of IL-1β,of
R1250 T1462 T1461 arg2Of increased,are
R1251 T1462 T1463 arg1Of increased,in
R1252 T1462 T1473 arg1Of increased,","
R1253 T1462 T1474 arg1Of increased,[
R1254 T1462 T1477 arg1Of increased,","
R1255 T1462 T1478 arg1Of increased,[
R1256 T1462 T1481 arg1Of increased,","
R1257 T1462 T1482 arg1Of increased,[
R1258 T1462 T1485 arg1Of increased,and
R1259 T1464 T1463 arg2Of BALF,in
R1260 T1464 T1465 arg1Of BALF,from
R1261 T1467 T1465 arg2Of patients,from
R1262 T1467 T1466 arg1Of patients,CF
R1263 T1467 T1468 arg1Of patients,with
R1264 T1469 T1468 arg2Of infection,with
R1265 T1469 T1470 arg1Of infection,[
R1266 T1471 T1470 arg2Of 21,[
R1267 T1472 T1470 arg3Of ],[
R1268 T1475 T1474 arg2Of 26,[
R1269 T1476 T1474 arg3Of ],[
R1270 T1479 T1478 arg2Of 27,[
R1271 T1480 T1478 arg3Of ],[
R1272 T1483 T1482 arg2Of 28,[
R1273 T1484 T1482 arg3Of ],[
R1274 T1487 T1486 arg1Of increase,this
R1275 T1487 T1488 arg1Of increase,has
R1276 T1487 T1489 arg1Of increase,been
R1277 T1487 T1491 arg2Of increase,associated
R1278 T1491 T1485 arg2Of associated,and
R1279 T1491 T1488 arg2Of associated,has
R1280 T1491 T1489 arg2Of associated,been
R1281 T1491 T1490 arg1Of associated,temporally
R1282 T1491 T1492 arg1Of associated,with
R1283 T1495 T1492 arg2Of response,with
R1284 T1495 T1493 arg1Of response,a
R1285 T1495 T1494 arg1Of response,clinical
R1286 T1495 T1496 arg1Of response,to
R1287 T1497 T1496 arg2Of treatment,to
R1288 T1497 T1498 arg1Of treatment,[
R1289 T1499 T1498 arg2Of 21,[
R1290 T1500 T1498 arg3Of ],[
R1291 T1501 T1502 arg1Of Polymorphisms,in
R1292 T1501 T1506 arg1Of Polymorphisms,have
R1293 T1501 T1508 arg1Of Polymorphisms,been
R1294 T1501 T1509 arg2Of Polymorphisms,associated
R1295 T1505 T1502 arg2Of gene,in
R1296 T1505 T1503 arg1Of gene,the
R1297 T1505 T1504 arg1Of gene,IL1B
R1298 T1509 T1506 arg2Of associated,have
R1299 T1509 T1507 arg1Of associated,also
R1300 T1509 T1508 arg2Of associated,been
R1301 T1509 T1510 arg1Of associated,with
R1302 T1509 T1519 arg1Of associated,[
R1303 T1512 T1510 arg2Of degrees,with
R1304 T1512 T1511 arg1Of degrees,varying
R1305 T1512 T1513 arg1Of degrees,of
R1306 T1512 T1516 arg1Of degrees,in
R1307 T1515 T1513 arg2Of severity,of
R1308 T1515 T1514 arg1Of severity,disease
R1309 T1518 T1516 arg2Of patients,in
R1310 T1518 T1517 arg1Of patients,CF
R1311 T1520 T1519 arg2Of 29,[
R1312 T1521 T1519 arg3Of ],[
R1313 T1523 T1522 arg1Of models,Murine
R1314 T1523 T1524 arg1Of models,of
R1315 T1523 T1527 arg1Of models,have
R1316 T1523 T1528 arg1Of models,exhibited
R1317 T1523 T1547 arg1Of models,support
R1318 T1526 T1524 arg2Of dysfunction,of
R1319 T1526 T1525 arg1Of dysfunction,CFTR
R1320 T1528 T1527 arg2Of exhibited,have
R1321 T1528 T1541 arg1Of exhibited,","
R1322 T1528 T1542 arg1Of exhibited,[
R1323 T1528 T1546 arg1Of exhibited,and
R1324 T1530 T1528 arg2Of increases,exhibited
R1325 T1530 T1529 arg1Of increases,significant
R1326 T1530 T1531 arg1Of increases,in
R1327 T1533 T1534 arg1Of expression,or
R1328 T1534 T1531 arg2Of or,in
R1329 T1534 T1532 arg1Of or,IL-1β
R1330 T1534 T1536 arg1Of or,in
R1331 T1535 T1534 arg2Of secretion,or
R1332 T1537 T1536 arg2Of macrophages,in
R1333 T1537 T1538 arg1Of macrophages,[
R1334 T1539 T1538 arg2Of 30,[
R1335 T1540 T1538 arg3Of ],[
R1336 T1543 T1542 arg2Of 31,[
R1337 T1544 T1542 arg3Of ],[
R1338 T1546 T1545 arg1Of and,","
R1339 T1547 T1546 arg2Of support,and
R1340 T1547 T1574 arg1Of support,","
R1341 T1547 T1575 arg1Of support,[
R1342 T1549 T1547 arg2Of hypothesis,support
R1343 T1549 T1548 arg1Of hypothesis,the
R1344 T1552 T1551 arg1Of loss,the
R1345 T1552 T1553 arg1Of loss,of
R1346 T1552 T1555 arg1Of loss,increases
R1347 T1554 T1553 arg2Of CFTR,of
R1348 T1555 T1549 arg2Of increases,hypothesis
R1349 T1555 T1550 arg1Of increases,that
R1350 T1555 T1558 arg1Of increases,under
R1351 T1555 T1566 arg1Of increases,","
R1352 T1555 T1567 arg1Of increases,[
R1353 T1555 T1570 arg1Of increases,","
R1354 T1555 T1571 arg1Of increases,[
R1355 T1557 T1555 arg2Of activation,increases
R1356 T1557 T1556 arg1Of activation,NF-κB
R1357 T1559 T1560 arg1Of basal,and
R1358 T1561 T1560 arg2Of stimulatory,and
R1359 T1562 T1558 arg2Of conditions,under
R1360 T1562 T1559 arg1Of conditions,basal
R1361 T1562 T1561 arg1Of conditions,stimulatory
R1362 T1562 T1563 arg1Of conditions,[
R1363 T1564 T1563 arg2Of 4,[
R1364 T1565 T1563 arg3Of ],[
R1365 T1568 T1567 arg2Of 5,[
R1366 T1569 T1567 arg3Of ],[
R1367 T1572 T1571 arg2Of 32,[
R1368 T1573 T1571 arg3Of ],[
R1369 T1576 T1575 arg2Of 33,[
R1370 T1577 T1575 arg3Of ],[
R1371 T1580 T1581 arg1Of replacement,of
R1372 T1580 T1592 arg1Of replacement,increases
R1373 T1583 T1581 arg2Of ions,of
R1374 T1583 T1582 arg1Of ions,chloride
R1375 T1583 T1584 arg1Of ions,with
R1376 T1585 T1586 arg1Of glutamate,or
R1377 T1586 T1584 arg2Of or,with
R1378 T1586 T1588 arg1Of or,in
R1379 T1587 T1586 arg2Of gluconate,or
R1380 T1591 T1588 arg2Of media,in
R1381 T1591 T1589 arg1Of media,cell
R1382 T1591 T1590 arg1Of media,culture
R1383 T1592 T1578 arg1Of increases,Finally
R1384 T1592 T1579 arg1Of increases,","
R1385 T1592 T1596 arg1Of increases,in
R1386 T1592 T1610 arg1Of increases,","
R1387 T1592 T1611 modOf increases,implying
R1388 T1593 T1592 arg2Of secretion,increases
R1389 T1593 T1594 arg1Of secretion,of
R1390 T1595 T1594 arg2Of IL-1β,of
R1391 T1597 T1596 arg2Of response,in
R1392 T1597 T1598 arg1Of response,to
R1393 T1600 T1598 arg2Of stimulation,to
R1394 T1600 T1599 arg1Of stimulation,NLRP3
R1395 T1600 T1601 arg1Of stimulation,by
R1396 T1603 T1601 arg2Of triphosphate,by
R1397 T1603 T1602 arg1Of triphosphate,adenosine
R1398 T1603 T1604 arg1Of triphosphate,(
R1399 T1603 T1607 arg1Of triphosphate,[
R1400 T1605 T1604 arg2Of ATP,(
R1401 T1606 T1604 arg3Of ),(
R1402 T1608 T1607 arg2Of 34,[
R1403 T1609 T1607 arg3Of ],[
R1404 T1614 T1611 arg2Of role,implying
R1405 T1614 T1612 arg1Of role,an
R1406 T1614 T1613 arg1Of role,inhibitory
R1407 T1614 T1615 arg1Of role,for
R1408 T1614 T1618 arg1Of role,in
R1409 T1617 T1615 arg2Of chloride,for
R1410 T1617 T1616 arg1Of chloride,extracellular
R1411 T1620 T1618 arg2Of activation,in
R1412 T1620 T1619 arg1Of activation,NLRP3
R1413 T1621 T1622 arg1Of Taken,together
R1414 T1625 T1624 arg1Of data,these
R1415 T1625 T1626 arg1Of data,implicate
R1416 T1626 T1621 modOf implicate,Taken
R1417 T1626 T1623 arg1Of implicate,","
R1418 T1626 T1636 arg1Of implicate,","
R1419 T1626 T1637 arg1Of implicate,in
R1420 T1628 T1626 arg2Of involvement,implicate
R1421 T1628 T1627 arg1Of involvement,the
R1422 T1628 T1629 arg1Of involvement,of
R1423 T1628 T1632 arg1Of involvement,consequently
R1424 T1628 T1633 arg1Of involvement,","
R1425 T1629 T1631 arg1Of of,and
R1426 T1630 T1629 arg2Of IL-1β,of
R1427 T1632 T1631 arg2Of consequently,and
R1428 T1635 T1633 arg2Of inflammasomes,","
R1429 T1635 T1634 arg1Of inflammasomes,the
R1430 T1640 T1637 arg2Of disease,in
R1431 T1640 T1638 arg1Of disease,CF
R1432 T1640 T1639 arg1Of disease,inflammatory
R2233 T2612 T2611 arg1Of epithelial,Airway
R2234 T2613 T2612 arg1Of cells,epithelial
R2235 T2613 T2614 arg1Of cells,do
R2236 T2613 T2616 arg1Of cells,produce
R2237 T2616 T2614 arg2Of produce,do
R2238 T2616 T2615 arg1Of produce,not
R2239 T2616 T2621 arg1Of produce,in
R2240 T2618 T2616 arg2Of amounts,produce
R2241 T2618 T2617 arg1Of amounts,significant
R2242 T2618 T2619 arg1Of amounts,of
R2243 T2620 T2619 arg2Of IL-1β,of
R2244 T2621 T2623 arg1Of in,to
R2245 T2622 T2621 arg2Of response,in
R2246 T2625 T2621 arg3Of stimulation,in
R2247 T2625 T2624 arg1Of stimulation,inflammasome
R2248 T2627 T2626 arg1Of inflammasomes,The
R2249 T2627 T2628 arg1Of inflammasomes,and
R2250 T2628 T2636 arg1Of and,are
R2251 T2628 T2637 arg2Of and,listed
R2252 T2631 T2628 arg2Of activators,and
R2253 T2631 T2629 arg1Of activators,their
R2254 T2631 T2630 arg1Of activators,respective
R2255 T2631 T2632 arg2Of activators,examined
R2256 T2632 T2633 arg1Of examined,in
R2257 T2635 T2633 arg2Of study,in
R2258 T2635 T2634 arg1Of study,this
R2259 T2637 T2636 arg2Of listed,are
R2260 T2637 T2638 arg1Of listed,in
R2261 T2640 T2638 arg2Of 1,in
R2262 T2640 T2639 arg1Of 1,Table
R2263 T2641 T2642 arg1Of Cells,were
R2264 T2641 T2643 arg2Of Cells,stimulated
R2265 T2643 T2642 arg2Of stimulated,were
R2266 T2643 T2644 arg1Of stimulated,in
R2267 T2645 T2644 arg2Of accordance,in
R2268 T2645 T2646 arg1Of accordance,with
R2269 T2648 T2646 arg2Of schedule,with
R2270 T2648 T2647 arg1Of schedule,the
R2271 T2648 T2649 arg1Of schedule,in
R2272 T2650 T2649 arg2Of Figure,in
R2273 T2650 T2651 arg1Of Figure,1
R2274 T2653 T2652 arg2Of CF,In
R2275 T2657 T2655 arg1Of cells,airway
R2276 T2657 T2656 arg1Of cells,epithelial
R2277 T2657 T2658 arg1Of cells,have
R2278 T2657 T2659 arg1Of cells,been
R2279 T2657 T2660 arg2Of cells,shown
R2280 T2657 T2662 arg1Of cells,possess
R2281 T2657 T2667 arg1Of cells,produce
R2282 T2660 T2652 arg1Of shown,In
R2283 T2660 T2654 arg1Of shown,","
R2284 T2660 T2658 arg2Of shown,have
R2285 T2660 T2659 arg2Of shown,been
R2286 T2662 T2666 arg1Of possess,and
R2287 T2665 T2662 arg2Of phenotype,possess
R2288 T2665 T2663 arg1Of phenotype,a
R2289 T2665 T2664 arg1Of phenotype,hyper-inflammatory
R2290 T2666 T2660 arg3Of and,shown
R2291 T2666 T2661 arg1Of and,to
R2292 T2667 T2666 arg2Of produce,and
R2293 T2667 T2676 arg1Of produce,","
R2294 T2667 T2677 arg1Of produce,[
R2295 T2672 T2667 arg2Of response,produce
R2296 T2672 T2668 arg1Of response,an
R2297 T2672 T2669 arg1Of response,exaggerated
R2298 T2672 T2670 arg1Of response,pro-inflammatory
R2299 T2672 T2671 arg1Of response,cytokine
R2300 T2672 T2673 arg1Of response,[
R2301 T2674 T2673 arg2Of 4,[
R2302 T2675 T2673 arg3Of ],[
R2303 T2678 T2677 arg2Of 5,[
R2304 T2679 T2677 arg3Of ],[
R2305 T2681 T2680 arg1Of determine,To
R2306 T2685 T2683 arg1Of cells,airway
R2307 T2685 T2684 arg1Of cells,epithelial
R2308 T2685 T2686 arg1Of cells,contribute
R2309 T2686 T2681 arg2Of contribute,determine
R2310 T2686 T2682 arg1Of contribute,if
R2311 T2686 T2687 arg1Of contribute,to
R2312 T2691 T2687 arg2Of production,to
R2313 T2691 T2688 arg1Of production,the
R2314 T2691 T2689 arg2Of production,increased
R2315 T2691 T2690 arg1Of production,IL-1β
R2316 T2691 T2692 arg1Of production,in
R2317 T2693 T2692 arg2Of patients,in
R2318 T2693 T2694 arg1Of patients,with
R2319 T2695 T2694 arg2Of CF,with
R2320 T2697 T2698 arg1Of CF,and
R2321 T2699 T2698 arg2Of control,and
R2322 T2703 T2697 arg1Of lines,CF
R2323 T2703 T2699 arg1Of lines,control
R2324 T2703 T2700 arg1Of lines,bronchial
R2325 T2703 T2701 arg1Of lines,epithelial
R2326 T2703 T2702 arg1Of lines,cell
R2327 T2703 T2704 arg1Of lines,were
R2328 T2703 T2705 arg2Of lines,stimulated
R2329 T2705 T2680 modOf stimulated,To
R2330 T2705 T2696 arg1Of stimulated,","
R2331 T2705 T2704 arg2Of stimulated,were
R2332 T2705 T2706 arg1Of stimulated,with
R2333 T2709 T2708 arg1Of inducers,inflammasome
R2334 T2713 T2710 arg1Of PAO1,P.
R2335 T2713 T2711 arg1Of PAO1,aeruginosa
R2336 T2713 T2712 arg1Of PAO1,strain
R2337 T2713 T2714 arg1Of PAO1,(
R2338 T2713 T2717 arg1Of PAO1,and
R2339 T2715 T2714 arg2Of PAO1,(
R2340 T2716 T2714 arg3Of ),(
R2341 T2717 T2706 arg2Of and,with
R2342 T2717 T2707 arg1Of and,the
R2343 T2717 T2709 arg1Of and,inducers
R2344 T2718 T2717 arg2Of LPS,and
R2345 T2718 T2719 arg2Of LPS,followed
R2346 T2721 T2719 arg1Of ATP,followed
R2347 T2721 T2720 arg2Of ATP,by
R2348 T2723 T2722 arg1Of levels,IL-1β
R2349 T2723 T2724 arg1Of levels,in
R2350 T2723 T2728 arg1Of levels,were
R2351 T2723 T2731 arg2Of levels,increased
R2352 T2727 T2724 arg2Of supernatants,in
R2353 T2727 T2725 arg1Of supernatants,cell
R2354 T2727 T2726 arg1Of supernatants,culture
R2355 T2731 T2728 arg2Of increased,were
R2356 T2731 T2729 arg1Of increased,not
R2357 T2731 T2730 arg1Of increased,greatly
R2358 T2731 T2732 arg1Of increased,in
R2359 T2731 T2744 arg1Of increased,","
R2360 T2731 T2745 arg1Of increased,although
R2361 T2735 T2736 arg1Of CF,or
R2362 T2737 T2736 arg2Of control,or
R2363 T2739 T2732 arg2Of lines,in
R2364 T2739 T2733 arg1Of lines,either
R2365 T2739 T2734 arg1Of lines,the
R2366 T2739 T2735 arg1Of lines,CF
R2367 T2739 T2737 arg1Of lines,control
R2368 T2739 T2738 arg1Of lines,cell
R2369 T2739 T2740 arg1Of lines,(
R2370 T2742 T2740 arg2Of 2a–d,(
R2371 T2742 T2741 arg1Of 2a–d,Fig.
R2372 T2743 T2740 arg3Of ),(
R2373 T2748 T2746 arg1Of increase,a
R2374 T2748 T2747 arg1Of increase,small
R2375 T2748 T2749 arg1Of increase,in
R2376 T2748 T2752 arg1Of increase,was
R2377 T2748 T2753 arg2Of increase,detected
R2378 T2751 T2749 arg2Of production,in
R2379 T2751 T2750 arg1Of production,IL-1β
R2380 T2753 T2745 arg2Of detected,although
R2381 T2753 T2752 arg2Of detected,was
R2382 T2753 T2754 arg1Of detected,in
R2383 T2753 T2762 arg1Of detected,in
R2384 T2753 T2767 arg1Of detected,","
R2385 T2753 T2768 arg1Of detected,by
R2386 T2754 T2760 arg1Of in,but
R2387 T2755 T2756 arg1Of NuLi-1,and
R2388 T2756 T2754 arg2Of and,in
R2389 T2758 T2756 arg2Of cells,and
R2390 T2758 T2757 arg1Of cells,CuFi-1
R2391 T2760 T2759 arg1Of but,","
R2392 T2762 T2760 arg2Of in,but
R2393 T2762 T2761 arg1Of in,not
R2394 T2763 T2764 arg1Of S9,and
R2395 T2765 T2764 arg2Of IB3-1,and
R2396 T2766 T2762 arg2Of cells,in
R2397 T2766 T2763 arg1Of cells,S9
R2398 T2766 T2765 arg1Of cells,IB3-1
R2399 T2770 T2768 arg2Of hours,by
R2400 T2770 T2769 arg1Of hours,24
R2401 T2772 T2771 arg2Of contrast,In
R2402 T2776 T2774 arg1Of cells,these
R2403 T2776 T2775 arg1Of cells,airway
R2404 T2776 T2777 arg1Of cells,were
R2405 T2776 T2779 arg1Of cells,responsive
R2406 T2777 T2771 arg1Of were,In
R2407 T2777 T2773 arg1Of were,","
R2408 T2777 T2789 arg1Of were,","
R2409 T2777 T2790 modOf were,producing
R2410 T2779 T2777 arg2Of responsive,were
R2411 T2779 T2778 arg1Of responsive,highly
R2412 T2779 T2780 arg1Of responsive,to
R2413 T2783 T2780 arg2Of stimuli,to
R2414 T2783 T2781 arg1Of stimuli,other
R2415 T2783 T2782 arg1Of stimuli,inflammatory
R2416 T2783 T2784 arg1Of stimuli,","
R2417 T2783 T2786 arg1Of stimuli,as
R2418 T2786 T2785 arg1Of as,such
R2419 T2788 T2786 arg2Of IL-1β,as
R2420 T2788 T2787 arg1Of IL-1β,recombinant
R2421 T2790 T2795 arg1Of producing,(
R2422 T2792 T2790 arg2Of quantities,producing
R2423 T2792 T2791 arg1Of quantities,large
R2424 T2792 T2793 arg1Of quantities,of
R2425 T2794 T2793 arg2Of IL-8,of
R2426 T2796 T2797 arg1Of Fig.,2a–d
R2427 T2798 T2795 arg2Of inserts,(
R2428 T2798 T2796 arg1Of inserts,Fig.
R2429 T2799 T2795 arg3Of ),(
R2671 T3097 T3096 arg1Of cells,epithelial
R2672 T3097 T3098 arg1Of cells,do
R2673 T3097 T3101 arg1Of cells,upregulate
R2674 T3101 T3095 arg1Of upregulate,Airway
R2675 T3101 T3098 arg2Of upregulate,do
R2676 T3101 T3099 arg1Of upregulate,not
R2677 T3101 T3100 arg1Of upregulate,significantly
R2678 T3101 T3104 arg1Of upregulate,in
R2679 T3103 T3101 arg2Of activity,upregulate
R2680 T3103 T3102 arg1Of activity,caspase-1
R2681 T3105 T3104 arg2Of response,in
R2682 T3105 T3106 arg1Of response,to
R2683 T3108 T3106 arg2Of stimulation,to
R2684 T3108 T3107 arg1Of stimulation,inflammasome
R2685 T3110 T3109 arg1Of examine,To
R2686 T3113 T3112 arg1Of activation,inflammasome
R2687 T3113 T3114 arg1Of activation,occurs
R2688 T3114 T3110 arg2Of occurs,examine
R2689 T3114 T3111 arg1Of occurs,if
R2690 T3114 T3115 arg1Of occurs,in
R2691 T3118 T3115 arg2Of cells,in
R2692 T3118 T3116 arg1Of cells,these
R2693 T3118 T3117 arg1Of cells,airway
R2694 T3121 T3120 arg1Of activity,caspase-1
R2695 T3121 T3122 arg1Of activity,was
R2696 T3121 T3123 arg2Of activity,quantified
R2697 T3123 T3109 modOf quantified,To
R2698 T3123 T3119 arg1Of quantified,","
R2699 T3123 T3122 arg2Of quantified,was
R2700 T3126 T3123 arg1Of cytometry,quantified
R2701 T3126 T3124 arg2Of cytometry,by
R2702 T3126 T3125 arg1Of cytometry,flow
R2703 T3127 T3128 arg1Of There,was
R2704 T3128 T3132 arg1Of was,upon
R2705 T3131 T3128 arg2Of increase,was
R2706 T3131 T3129 arg1Of increase,no
R2707 T3131 T3130 arg1Of increase,significant
R2708 T3133 T3132 arg2Of stimulation,upon
R2709 T3133 T3134 arg1Of stimulation,with
R2710 T3133 T3139 arg1Of stimulation,at
R2711 T3136 T3137 arg1Of PAO1,or
R2712 T3137 T3134 arg2Of or,with
R2713 T3137 T3135 arg1Of or,live
R2714 T3138 T3137 arg2Of LPS+ATP,or
R2715 T3141 T3139 arg2Of times,at
R2716 T3141 T3140 arg1Of times,the
R2717 T3141 T3142 arg2Of times,examined
R2718 T3142 T3143 arg1Of examined,(
R2719 T3145 T3143 arg2Of 3a–b,(
R2720 T3145 T3144 arg1Of 3a–b,Fig.
R2721 T3146 T3143 arg3Of ),(
R2722 T3149 T3148 arg1Of studies,previous
R2723 T3149 T3150 arg1Of studies,have
R2724 T3149 T3151 arg1Of studies,indicated
R2725 T3151 T3147 arg2Of indicated,Because
R2726 T3151 T3150 arg2Of indicated,have
R2727 T3153 T3151 arg2Of role,indicated
R2728 T3153 T3152 arg1Of role,a
R2729 T3153 T3154 arg1Of role,for
R2730 T3153 T3156 arg1Of role,in
R2731 T3155 T3154 arg2Of caspase-1,for
R2732 T3158 T3156 arg2Of activation,in
R2733 T3158 T3157 arg1Of activation,the
R2734 T3158 T3159 arg1Of activation,of
R2735 T3158 T3161 arg1Of activation,through
R2736 T3160 T3159 arg2Of NF-κB,of
R2737 T3163 T3162 arg1Of receptor,Toll-like
R2738 T3163 T3164 arg1Of receptor,(
R2739 T3165 T3164 arg2Of TLR,(
R2740 T3166 T3164 arg3Of ),(
R2741 T3167 T3161 arg2Of signaling,through
R2742 T3167 T3163 arg1Of signaling,receptor
R2743 T3167 T3168 arg1Of signaling,[
R2744 T3169 T3168 arg2Of 35,[
R2745 T3170 T3168 arg3Of ],[
R2746 T3172 T3173 arg1Of we,examined
R2747 T3173 T3147 arg1Of examined,Because
R2748 T3173 T3171 arg1Of examined,","
R2749 T3176 T3175 arg1Of inhibition,chemical
R2750 T3176 T3177 arg1Of inhibition,of
R2751 T3176 T3179 arg1Of inhibition,altered
R2752 T3178 T3177 arg2Of caspase-1,of
R2753 T3179 T3173 arg2Of altered,examined
R2754 T3179 T3174 arg1Of altered,whether
R2755 T3179 T3183 arg1Of altered,in
R2756 T3182 T3179 arg2Of production,altered
R2757 T3182 T3180 arg1Of production,NF-κB-dependent
R2758 T3182 T3181 arg1Of production,IL-6
R2759 T3184 T3183 arg2Of response,in
R2760 T3184 T3185 arg1Of response,to
R2761 T3187 T3185 arg2Of aeruginosa,to
R2762 T3187 T3186 arg1Of aeruginosa,P.
R2763 T3190 T3191 arg1Of treatment,with
R2764 T3190 T3199 arg1Of treatment,did
R2765 T3190 T3201 arg1Of treatment,decrease
R2766 T3195 T3191 arg2Of z-YVAD-fmk,with
R2767 T3195 T3192 arg1Of z-YVAD-fmk,the
R2768 T3195 T3193 arg1Of z-YVAD-fmk,caspase-1
R2769 T3195 T3194 arg1Of z-YVAD-fmk,inhibitor
R2770 T3195 T3196 arg1Of z-YVAD-fmk,(
R2771 T3197 T3196 arg2Of YVAD,(
R2772 T3198 T3196 arg3Of ),(
R2773 T3201 T3188 arg1Of decrease,However
R2774 T3201 T3189 arg1Of decrease,","
R2775 T3201 T3199 arg2Of decrease,did
R2776 T3201 T3200 arg1Of decrease,not
R2777 T3201 T3204 arg1Of decrease,by
R2778 T3203 T3201 arg2Of secretion,decrease
R2779 T3203 T3202 arg1Of secretion,IL-6
R2780 T3207 T3204 arg2Of cells,by
R2781 T3207 T3205 arg1Of cells,airway
R2782 T3207 T3206 arg1Of cells,epithelial
R2783 T3207 T3208 arg1Of cells,(
R2784 T3210 T3208 arg2Of 3c,(
R2785 T3210 T3209 arg1Of 3c,Fig.
R2786 T3211 T3208 arg3Of ),(
R2938 T3406 T3404 arg1Of monocytes,CD14
R2939 T3406 T3405 arg1Of monocytes,positive
R2940 T3406 T3407 arg1Of monocytes,from
R2941 T3406 T3412 arg1Of monocytes,show
R2942 T3409 T3408 arg1Of patients,CF
R2943 T3409 T3410 arg1Of patients,and
R2944 T3410 T3407 arg2Of and,from
R2945 T3411 T3410 arg2Of controls,and
R2946 T3414 T3412 arg2Of increases,show
R2947 T3414 T3413 arg1Of increases,similar
R2948 T3414 T3415 arg1Of increases,in
R2949 T3417 T3415 arg2Of activity,in
R2950 T3417 T3416 arg1Of activity,caspase-1
R2951 T3417 T3418 arg1Of activity,upon
R2952 T3420 T3418 arg2Of stimulation,upon
R2953 T3420 T3419 arg1Of stimulation,inflammasome
R2954 T3421 T3422 arg1Of Monocytes,were
R2955 T3421 T3423 arg2Of Monocytes,identified
R2956 T3423 T3422 arg2Of identified,were
R2957 T3423 T3424 arg1Of identified,in
R2958 T3426 T3424 arg2Of populations,in
R2959 T3426 T3425 arg1Of populations,PBMC
R2960 T3427 T3423 arg3Of using,identified
R2961 T3427 T3429 arg1Of using,as
R2962 T3428 T3427 arg2Of CD14,using
R2963 T3432 T3429 arg2Of marker,as
R2964 T3432 T3430 arg1Of marker,a
R2965 T3432 T3431 arg1Of marker,phenotyping
R2966 T3435 T3433 arg1Of monocytes,CD14
R2967 T3435 T3434 arg1Of monocytes,positive
R2968 T3435 T3436 arg1Of monocytes,from
R2969 T3435 T3442 arg1Of monocytes,showed
R2970 T3438 T3437 arg1Of patients,CF
R2971 T3438 T3439 arg1Of patients,and
R2972 T3439 T3436 arg2Of and,from
R2973 T3441 T3439 arg2Of controls,and
R2974 T3441 T3440 arg1Of controls,healthy
R2975 T3442 T3467 arg1Of showed,but
R2976 T3445 T3442 arg2Of increase,showed
R2977 T3445 T3443 arg1Of increase,a
R2978 T3445 T3444 arg1Of increase,significant
R2979 T3445 T3446 arg1Of increase,in
R2980 T3445 T3449 arg1Of increase,upon
R2981 T3445 T3451 arg1Of increase,with
R2982 T3448 T3446 arg2Of activation,in
R2983 T3448 T3447 arg1Of activation,caspase-1
R2984 T3450 T3449 arg2Of stimulation,upon
R2985 T3452 T3453 arg1Of LPS+ATP,","
R2986 T3453 T3456 arg1Of ",",and
R2987 T3454 T3453 arg2Of PAO1,","
R2988 T3456 T3451 arg2Of and,with
R2989 T3456 T3455 arg1Of and,","
R2990 T3456 T3458 arg1Of and,(
R2991 T3456 T3463 arg1Of and,(
R2992 T3457 T3456 arg2Of LPS+Poly,and
R2993 T3459 T3458 arg2Of dA,(
R2994 T3459 T3460 arg1Of dA,:
R2995 T3459 T3461 arg1Of dA,dT
R2996 T3462 T3458 arg3Of ),(
R2997 T3465 T3463 arg2Of 4a,(
R2998 T3465 T3464 arg1Of 4a,Fig.
R2999 T3466 T3463 arg3Of ),(
R3000 T3469 T3468 arg1Of activation,this
R3001 T3469 T3470 arg1Of activation,was
R3002 T3469 T3472 arg1Of activation,different
R3003 T3470 T3467 arg2Of was,but
R3004 T3470 T3471 arg1Of was,not
R3005 T3472 T3470 arg2Of different,was
R3006 T3472 T3473 arg1Of different,between
R3007 T3474 T3475 arg1Of CF,and
R3008 T3476 T3475 arg2Of control,and
R3009 T3477 T3473 arg2Of subjects,between
R3010 T3477 T3474 arg1Of subjects,CF
R3011 T3477 T3476 arg1Of subjects,control
R3012 T3477 T3478 arg1Of subjects,(
R3013 T3480 T3478 arg2Of 4b,(
R3014 T3480 T3479 arg1Of 4b,Fig.
R3015 T3481 T3478 arg3Of ),(
R3130 T3638 T3639 arg1Of PBMCs,from
R3131 T3638 T3642 arg1Of PBMCs,do
R3132 T3638 T3644 arg1Of PBMCs,produce
R3133 T3641 T3639 arg2Of patients,from
R3134 T3641 T3640 arg1Of patients,CF
R3135 T3644 T3642 arg2Of produce,do
R3136 T3644 T3643 arg1Of produce,not
R3137 T3644 T3649 arg1Of produce,upon
R3138 T3646 T3644 arg2Of amounts,produce
R3139 T3646 T3645 arg2Of amounts,increased
R3140 T3646 T3647 arg1Of amounts,of
R3141 T3648 T3647 arg2Of IL-1β,of
R3142 T3651 T3649 arg2Of stimulation,upon
R3143 T3651 T3650 arg1Of stimulation,inflammasome
R3144 T3653 T3652 arg1Of studies,Previous
R3145 T3653 T3654 arg1Of studies,have
R3146 T3653 T3655 arg1Of studies,shown
R3147 T3655 T3654 arg2Of shown,have
R3148 T3655 T3681 arg1Of shown,","
R3149 T3655 T3682 arg1Of shown,[
R3150 T3655 T3685 arg1Of shown,","
R3151 T3655 T3686 arg1Of shown,[
R3152 T3658 T3657 arg1Of loss,the
R3153 T3658 T3659 arg1Of loss,of
R3154 T3658 T3661 arg1Of loss,results
R3155 T3660 T3659 arg2Of CFTR,of
R3156 T3661 T3655 arg2Of results,shown
R3157 T3661 T3656 arg1Of results,that
R3158 T3661 T3662 arg1Of results,in
R3159 T3661 T3673 arg1Of results,","
R3160 T3661 T3674 arg1Of results,[
R3161 T3661 T3677 arg1Of results,","
R3162 T3661 T3678 arg1Of results,[
R3163 T3665 T3663 arg2Of activity,increased
R3164 T3665 T3664 arg1Of activity,NF-κB
R3165 T3665 T3666 arg1Of activity,and
R3166 T3666 T3662 arg2Of and,in
R3167 T3669 T3666 arg2Of secretion,and
R3168 T3669 T3667 arg1Of secretion,pro-inflammatory
R3169 T3669 T3668 arg1Of secretion,cytokine
R3170 T3669 T3670 arg1Of secretion,[
R3171 T3671 T3670 arg2Of 4,[
R3172 T3672 T3670 arg3Of ],[
R3173 T3675 T3674 arg2Of 5,[
R3174 T3676 T3674 arg3Of ],[
R3175 T3679 T3678 arg2Of 32,[
R3176 T3680 T3678 arg3Of ],[
R3177 T3683 T3682 arg2Of 36,[
R3178 T3684 T3682 arg3Of ],[
R3179 T3687 T3686 arg2Of 37,[
R3180 T3688 T3686 arg3Of ],[
R3181 T3691 T3689 arg1Of examine,To
R3182 T3691 T3690 arg1Of examine,further
R3183 T3693 T3691 arg2Of relationship,examine
R3184 T3693 T3692 arg1Of relationship,this
R3185 T3695 T3691 arg1Of PBMCs,examine
R3186 T3695 T3696 arg1Of PBMCs,from
R3187 T3695 T3703 arg1Of PBMCs,were
R3188 T3695 T3704 arg2Of PBMCs,stimulated
R3189 T3698 T3697 arg1Of patients,CF
R3190 T3698 T3699 arg1Of patients,and
R3191 T3699 T3696 arg2Of and,from
R3192 T3701 T3700 arg1Of adult,healthy
R3193 T3702 T3699 arg2Of controls,and
R3194 T3702 T3701 arg1Of controls,adult
R3195 T3704 T3689 modOf stimulated,To
R3196 T3704 T3694 arg1Of stimulated,","
R3197 T3704 T3703 arg2Of stimulated,were
R3198 T3704 T3705 arg1Of stimulated,with
R3199 T3706 T3707 arg1Of PAO1,","
R3200 T3707 T3710 arg1Of ",",and
R3201 T3708 T3707 arg2Of LPS+ATP,","
R3202 T3710 T3705 arg2Of and,with
R3203 T3710 T3709 arg1Of and,","
R3204 T3710 T3717 arg1Of and,","
R3205 T3711 T3710 arg2Of LPS+Poly,and
R3206 T3711 T3712 arg1Of LPS+Poly,(
R3207 T3713 T3712 arg2Of dA,(
R3208 T3713 T3714 arg1Of dA,:
R3209 T3713 T3715 arg1Of dA,dT
R3210 T3716 T3712 arg3Of ),(
R3211 T3719 T3717 arg2Of activate,","
R3212 T3719 T3718 arg1Of activate,to
R3213 T3719 T3728 arg1Of activate,","
R3214 T3719 T3729 arg1Of activate,respectively
R3215 T3721 T3722 arg1Of NLRC4,","
R3216 T3722 T3725 arg1Of ",",and
R3217 T3723 T3722 arg2Of NLRP3,","
R3218 T3725 T3724 arg1Of and,","
R3219 T3726 T3725 arg2Of AIM2,and
R3220 T3727 T3719 arg2Of inflammasomes,activate
R3221 T3727 T3720 arg1Of inflammasomes,the
R3222 T3727 T3721 arg1Of inflammasomes,NLRC4
R3223 T3727 T3723 arg1Of inflammasomes,NLRP3
R3224 T3727 T3726 arg1Of inflammasomes,AIM2
R3225 T3732 T3730 arg2Of hours,By
R3226 T3732 T3731 arg1Of hours,24
R3227 T3732 T3733 arg1Of hours,of
R3228 T3734 T3733 arg2Of stimulation,of
R3229 T3737 T3736 arg1Of PBMCs,CF
R3230 T3737 T3738 arg1Of PBMCs,did
R3231 T3737 T3740 arg1Of PBMCs,produce
R3232 T3737 T3756 arg1Of PBMCs,compared
R3233 T3740 T3730 arg1Of produce,By
R3234 T3740 T3735 arg1Of produce,","
R3235 T3740 T3738 arg2Of produce,did
R3236 T3740 T3739 arg1Of produce,not
R3237 T3740 T3755 arg1Of produce,when
R3238 T3742 T3740 arg2Of amounts,produce
R3239 T3742 T3741 arg2Of amounts,increased
R3240 T3742 T3743 arg1Of amounts,of
R3241 T3744 T3745 arg1Of IL-1β,(
R3242 T3744 T3749 arg1Of IL-1β,or
R3243 T3747 T3745 arg2Of 5a,(
R3244 T3747 T3746 arg1Of 5a,Fig.
R3245 T3748 T3745 arg3Of ),(
R3246 T3749 T3743 arg2Of or,of
R3247 T3750 T3749 arg2Of IL-8,or
R3248 T3750 T3751 arg1Of IL-8,(
R3249 T3753 T3751 arg2Of 5b,(
R3250 T3753 T3752 arg1Of 5b,Fig.
R3251 T3754 T3751 arg3Of ),(
R3252 T3756 T3755 arg2Of compared,when
R3253 T3756 T3757 arg1Of compared,to
R3254 T3759 T3757 arg2Of controls,to
R3255 T3759 T3758 arg1Of controls,healthy
R3256 T3762 T3763 arg1Of we,did
R3257 T3762 T3764 arg1Of we,notice
R3258 T3764 T3760 arg1Of notice,However
R3259 T3764 T3761 arg1Of notice,","
R3260 T3764 T3763 arg2Of notice,did
R3261 T3764 T3773 arg1Of notice,in
R3262 T3767 T3764 arg2Of decrease,notice
R3263 T3767 T3765 arg1Of decrease,a
R3264 T3767 T3766 arg1Of decrease,transient
R3265 T3767 T3768 arg1Of decrease,(
R3266 T3769 T3768 arg2Of P,(
R3267 T3769 T3770 arg1Of P,<
R3268 T3769 T3771 arg1Of P,0.001
R3269 T3772 T3768 arg3Of ),(
R3270 T3775 T3773 arg2Of amount,in
R3271 T3775 T3774 arg1Of amount,the
R3272 T3775 T3776 arg1Of amount,of
R3273 T3775 T3778 arg2Of amount,produced
R3274 T3777 T3776 arg2Of IL-1β,of
R3275 T3778 T3782 arg1Of produced,in
R3276 T3778 T3786 arg1Of produced,at
R3277 T3781 T3778 arg1Of cells,produced
R3278 T3781 T3779 arg2Of cells,by
R3279 T3781 T3780 arg1Of cells,CF
R3280 T3782 T3784 arg1Of in,to
R3281 T3783 T3782 arg2Of response,in
R3282 T3785 T3782 arg3Of LPS+ATP,in
R3283 T3788 T3786 arg2Of hours,at
R3284 T3788 T3787 arg1Of hours,6
R3285 T3788 T3789 arg1Of hours,(
R3286 T3790 T3789 arg2Of data,(
R3287 T3790 T3792 arg2Of data,shown
R3288 T3792 T3791 arg1Of shown,not
R3289 T3793 T3789 arg3Of ),(
R3290 T3794 T3795 arg1Of Stimulation,of
R3291 T3794 T3797 arg1Of Stimulation,with
R3292 T3794 T3800 arg1Of Stimulation,that
R3293 T3794 T3801 arg1Of Stimulation,lacks
R3294 T3794 T3815 arg1Of Stimulation,produced
R3295 T3796 T3795 arg2Of PBMCs,of
R3296 T3799 T3797 arg2Of aeruginosa,with
R3297 T3799 T3798 arg1Of aeruginosa,P.
R3298 T3802 T3801 arg2Of exsA,lacks
R3299 T3802 T3803 arg1Of exsA,(
R3300 T3802 T3806 arg1Of exsA,","
R3301 T3804 T3803 arg2Of PAO1ΔexsA,(
R3302 T3805 T3803 arg3Of ),(
R3303 T3809 T3806 arg2Of regulator,","
R3304 T3809 T3807 arg1Of regulator,a
R3305 T3809 T3808 arg1Of regulator,key
R3306 T3809 T3810 arg1Of regulator,of
R3307 T3813 T3810 arg2Of secretion,of
R3308 T3813 T3811 arg1Of secretion,type
R3309 T3813 T3812 arg1Of secretion,III
R3310 T3815 T3814 arg1Of produced,","
R3311 T3815 T3819 arg1Of produced,compared
R3312 T3815 T3825 arg1Of produced,by
R3313 T3817 T3816 arg1Of less,three-fold
R3314 T3818 T3815 arg2Of IL-1β,produced
R3315 T3818 T3817 arg1Of IL-1β,less
R3316 T3820 T3819 arg2Of to,compared
R3317 T3824 T3820 arg2Of strain,to
R3318 T3824 T3821 arg1Of strain,the
R3319 T3824 T3822 arg1Of strain,parental
R3320 T3824 T3823 arg1Of strain,PAO1
R3321 T3827 T3825 arg2Of hours,by
R3322 T3827 T3826 arg1Of hours,24
R3323 T3827 T3828 arg1Of hours,(
R3324 T3830 T3828 arg2Of 5a,(
R3325 T3830 T3829 arg1Of 5a,Fig.
R3326 T3831 T3828 arg3Of ),(
R3327 T3833 T3832 arg1Of stimulation,Inflammasome
R3328 T3833 T3834 arg1Of stimulation,without
R3329 T3833 T3836 arg1Of stimulation,did
R3330 T3833 T3838 arg1Of stimulation,result
R3331 T3835 T3834 arg2Of priming,without
R3332 T3838 T3836 arg2Of result,did
R3333 T3838 T3837 arg1Of result,not
R3334 T3838 T3839 arg1Of result,in
R3335 T3842 T3839 arg2Of production,in
R3336 T3842 T3840 arg1Of production,any
R3337 T3842 T3841 arg1Of production,IL-1β
R3338 T3842 T3843 arg1Of production,in
R3339 T3845 T3846 arg1Of CF,or
R3340 T3846 T3843 arg2Of or,in
R3341 T3846 T3844 arg1Of or,either
R3342 T3848 T3846 arg2Of PBMCs,or
R3343 T3848 T3847 arg1Of PBMCs,control
R3344 T3849 T3850 arg1Of Contrary,to
R3345 T3852 T3850 arg2Of hypothesis,to
R3346 T3852 T3851 arg1Of hypothesis,our
R3347 T3855 T3854 arg1Of results,these
R3348 T3855 T3856 arg1Of results,indicate
R3349 T3856 T3849 arg1Of indicate,Contrary
R3350 T3856 T3853 arg1Of indicate,","
R3351 T3858 T3859 arg1Of PBMCs,from
R3352 T3858 T3862 arg1Of PBMCs,do
R3353 T3858 T3864 arg1Of PBMCs,display
R3354 T3861 T3859 arg2Of patients,from
R3355 T3861 T3860 arg1Of patients,CF
R3356 T3864 T3862 arg2Of display,do
R3357 T3864 T3863 arg1Of display,not
R3358 T3864 T3874 arg1Of display,nor
R3359 T3866 T3864 arg2Of production,display
R3360 T3866 T3865 arg2Of production,increased
R3361 T3866 T3867 arg1Of production,of
R3362 T3866 T3871 arg1Of production,with
R3363 T3868 T3869 arg1Of IL-1β,or
R3364 T3869 T3867 arg2Of or,of
R3365 T3870 T3869 arg2Of IL-8,or
R3366 T3873 T3871 arg2Of activation,with
R3367 T3873 T3872 arg1Of activation,inflammasome
R3368 T3874 T3857 arg1Of nor,that
R3369 T3874 T3877 arg1Of nor,suggest
R3370 T3875 T3874 arg2Of do,nor
R3371 T3876 T3875 arg1Of they,do
R3372 T3877 T3856 arg2Of suggest,indicate
R3373 T3880 T3881 arg1Of basal,or
R3374 T3880 T3882 arg2Of basal,induced
R3375 T3884 T3877 arg2Of activity,suggest
R3376 T3884 T3878 arg1Of activity,any
R3377 T3884 T3879 arg2Of activity,increased
R3378 T3884 T3880 arg1Of activity,basal
R3379 T3884 T3883 arg1Of activity,NF-κB
R3380 T3886 T3885 arg1Of results,These
R3381 T3886 T3887 arg1Of results,are
R3382 T3886 T3888 arg1Of results,consistent
R3383 T3888 T3887 arg2Of consistent,are
R3384 T3888 T3889 arg1Of consistent,with
R3385 T3891 T3889 arg2Of observation,with
R3386 T3891 T3890 arg1Of observation,our
R3387 T3894 T3893 arg1Of activity,caspase-1
R3388 T3894 T3895 arg1Of activity,is
R3389 T3894 T3897 arg1Of activity,different
R3390 T3895 T3891 arg2Of is,observation
R3391 T3895 T3892 arg1Of is,that
R3392 T3895 T3896 arg1Of is,not
R3393 T3897 T3895 arg2Of different,is
R3394 T3897 T3898 arg1Of different,between
R3395 T3899 T3900 arg1Of CF,and
R3396 T3901 T3900 arg2Of control,and
R3397 T3902 T3898 arg2Of PBMCs,between
R3398 T3902 T3899 arg1Of PBMCs,CF
R3399 T3902 T3901 arg1Of PBMCs,control
R3400 T3902 T3903 arg1Of PBMCs,(
R3401 T3904 T3903 arg2Of Fig.,(
R3402 T3904 T3905 arg1Of Fig.,4
R3403 T3906 T3903 arg3Of ),(
R3794 T4393 T4392 arg1Of activation,NF-κB
R3795 T4393 T4394 arg1Of activation,is
R3796 T4393 T4395 arg2Of activation,required
R3797 T4395 T4394 arg2Of required,is
R3798 T4395 T4396 arg1Of required,for
R3799 T4397 T4398 arg1Of IL-1β,and
R3864 T4458 T4459 arg1Of secretion,(
R3865 T4461 T4459 arg2Of 6b,(
R3800 T4399 T4398 arg2Of IL-8,and
R3801 T4400 T4396 arg2Of responses,for
R3802 T4400 T4397 arg1Of responses,IL-1β
R3803 T4400 T4399 arg1Of responses,IL-8
R3804 T4400 T4401 arg1Of responses,to
R3805 T4403 T4401 arg2Of aeruginosa,to
R3806 T4403 T4402 arg1Of aeruginosa,P.
R3807 T4404 T4405 arg1Of We,confirmed
R3808 T4404 T4417 arg1Of We,using
R3809 T4405 T4417 modOf confirmed,using
R3810 T4407 T4405 arg2Of dependence,confirmed
R3811 T4407 T4406 arg1Of dependence,the
R3812 T4407 T4408 arg1Of dependence,of
R3813 T4407 T4414 arg1Of dependence,on
R3814 T4410 T4411 arg1Of IL-1β,and
R3815 T4412 T4411 arg2Of IL-8,and
R3816 T4413 T4408 arg2Of production,of
R3817 T4413 T4409 arg1Of production,PAO1-induced
R3818 T4413 T4410 arg1Of production,IL-1β
R3819 T4413 T4412 arg1Of production,IL-8
R3820 T4416 T4414 arg2Of activation,on
R3821 T4416 T4415 arg1Of activation,NF-κB
R3822 T4419 T4417 arg2Of cells,using
R3823 T4419 T4418 arg1Of cells,THP-1
R3824 T4419 T4420 arg1Of cells,expressing
R3825 T4422 T4420 arg2Of reporter,expressing
R3826 T4422 T4421 arg1Of reporter,a
R3827 T4422 T4423 arg2Of reporter,driven
R3828 T4425 T4426 arg1Of NF-κB,and
R3829 T4427 T4426 arg2Of AP-1,and
R3830 T4429 T4423 arg1Of elements,driven
R3831 T4429 T4424 arg2Of elements,by
R3832 T4429 T4425 arg1Of elements,NF-κB
R3833 T4429 T4427 arg1Of elements,AP-1
R3834 T4429 T4428 arg1Of elements,response
R3835 T4430 T4431 arg1Of We,found
R3836 T4431 T4470 arg1Of found,(
R3837 T4433 T4434 arg1Of stimulation,of
R3838 T4433 T4439 arg1Of stimulation,with
R3839 T4433 T4442 arg1Of stimulation,produced
R3840 T4438 T4434 arg2Of cells,of
R3841 T4438 T4435 arg2Of cells,primed
R3842 T4438 T4436 arg1Of cells,THP-1
R3843 T4438 T4437 arg1Of cells,reporter
R3844 T4441 T4439 arg2Of PAO1,with
R3845 T4441 T4440 arg1Of PAO1,heat-killed
R3846 T4442 T4453 arg1Of produced,and
R3847 T4445 T4442 arg2Of levels,produced
R3848 T4445 T4443 arg1Of levels,the
R3849 T4445 T4444 arg1Of levels,highest
R3850 T4445 T4446 arg1Of levels,of
R3851 T4448 T4446 arg2Of activation,of
R3852 T4448 T4447 arg1Of activation,NF-κB/AP-1
R3853 T4448 T4449 arg1Of activation,(
R3854 T4451 T4449 arg2Of 6a,(
R3855 T4451 T4450 arg1Of 6a,Fig.
R3856 T4452 T4449 arg3Of ),(
R3857 T4453 T4431 arg2Of and,found
R3858 T4453 T4432 arg1Of and,that
R3859 T4454 T4455 arg1Of this,correlated
R3860 T4455 T4456 arg1Of correlated,with
R3861 T4455 T4463 arg1Of correlated,but
R3862 T4458 T4456 arg2Of secretion,with
R3863 T4458 T4457 arg1Of secretion,IL-8
R3866 T4461 T4460 arg1Of 6b,Fig.
R3867 T4462 T4459 arg3Of ),(
R3868 T4463 T4453 arg2Of but,and
R3869 T4465 T4464 arg1Of amounts,negligible
R3870 T4465 T4466 arg1Of amounts,of
R3871 T4465 T4468 arg1Of amounts,were
R3872 T4465 T4469 arg2Of amounts,secreted
R3873 T4467 T4466 arg2Of IL-1β,of
R3874 T4469 T4463 arg2Of secreted,but
R3875 T4469 T4468 arg2Of secreted,were
R3876 T4472 T4470 arg2Of 6c,(
R3877 T4472 T4471 arg1Of 6c,Fig.
R3878 T4473 T4470 arg3Of ),(
R3879 T4474 T4475 arg1Of Stimulation,of
R3880 T4474 T4480 arg1Of Stimulation,with
R3881 T4474 T4483 arg1Of Stimulation,did
R3882 T4474 T4486 arg1Of Stimulation,increase
R3883 T4479 T4475 arg2Of cells,of
R3884 T4479 T4476 arg2Of cells,primed
R3885 T4479 T4477 arg1Of cells,THP-1
R3886 T4479 T4478 arg1Of cells,reporter
R3887 T4482 T4480 arg2Of PAO1,with
R3888 T4482 T4481 arg1Of PAO1,live
R3889 T4486 T4483 arg2Of increase,did
R3890 T4486 T4484 arg1Of increase,not
R3891 T4486 T4485 arg1Of increase,significantly
R3892 T4486 T4500 arg1Of increase,over
R3893 T4488 T4487 arg1Of activity,NF-κB/AP-1
R3894 T4488 T4489 arg1Of activity,(
R3895 T4488 T4493 arg1Of activity,or
R3896 T4491 T4489 arg2Of 6a,(
R3897 T4491 T4490 arg1Of 6a,Fig.
R3898 T4492 T4489 arg3Of ),(
R3899 T4493 T4486 arg2Of or,increase
R3900 T4495 T4493 arg2Of secretion,or
R3901 T4495 T4494 arg1Of secretion,IL-8
R3902 T4495 T4496 arg1Of secretion,(
R3903 T4498 T4496 arg2Of 6b,(
R3904 T4498 T4497 arg1Of 6b,Fig.
R3905 T4499 T4496 arg3Of ),(
R3906 T4501 T4500 arg2Of priming,over
R3907 T4501 T4502 arg1Of priming,alone
R3908 T4506 T4505 arg1Of production,IL-1β
R3909 T4506 T4507 arg1Of production,was
R3910 T4506 T4509 arg2Of production,augmented
R3911 T4509 T4503 arg1Of augmented,However
R3912 T4509 T4504 arg1Of augmented,","
R3913 T4509 T4507 arg2Of augmented,was
R3914 T4509 T4508 arg1Of augmented,greatly
R3915 T4509 T4510 arg1Of augmented,over
R3916 T4512 T4510 arg2Of cells,over
R3917 T4512 T4511 arg2Of cells,primed
R3918 T4512 T4513 arg2Of cells,stimulated
R3919 T4513 T4514 arg1Of stimulated,with
R3920 T4515 T4516 arg1Of heat-killed,PAO1
R3921 T4515 T4517 arg1Of heat-killed,or
R3922 T4518 T4517 arg2Of unprimed,or
R3923 T4519 T4514 arg2Of cells,with
R3924 T4519 T4515 arg1Of cells,heat-killed
R3925 T4519 T4518 arg1Of cells,unprimed
R3926 T4519 T4520 arg2Of cells,stimulated
R3927 T4520 T4521 arg1Of stimulated,with
R3928 T4523 T4521 arg2Of PAO1,with
R3929 T4523 T4522 arg1Of PAO1,live
R3930 T4523 T4524 arg1Of PAO1,(
R3931 T4526 T4524 arg2Of 6c,(
R3932 T4526 T4525 arg1Of 6c,Fig.
R3933 T4527 T4524 arg3Of ),(
R3934 T4528 T4529 arg1Of This,confirmed
R3935 T4532 T4531 arg1Of activation,NF-κB
R3936 T4532 T4533 arg1Of activation,alone
R3937 T4532 T4534 arg1Of activation,is
R3938 T4532 T4536 arg1Of activation,sufficient
R3939 T4534 T4535 arg1Of is,not
R3940 T4534 T4542 arg1Of is,but
R3941 T4536 T4534 arg2Of sufficient,is
R3942 T4536 T4537 arg1Of sufficient,for
R3943 T4540 T4537 arg2Of secretion,for
R3944 T4540 T4538 arg1Of secretion,maximal
R3945 T4540 T4539 arg1Of secretion,IL-1β
R3946 T4542 T4529 arg2Of but,confirmed
R3947 T4542 T4530 arg1Of but,that
R3948 T4542 T4541 arg1Of but,","
R3949 T4544 T4543 arg2Of priming,increased
R3950 T4544 T4545 arg1Of priming,of
R3951 T4544 T4547 arg1Of priming,is
R3952 T4544 T4548 arg1Of priming,capable
R3953 T4544 T4550 arg1Of priming,augmenting
R3954 T4546 T4545 arg2Of NF-κB,of
R3955 T4547 T4542 arg2Of is,but
R3956 T4548 T4547 arg2Of capable,is
R3957 T4548 T4549 arg1Of capable,of
R3958 T4550 T4549 arg2Of augmenting,of
R3959 T4550 T4555 arg1Of augmenting,upon
R3960 T4552 T4551 arg1Of production,IL-1β
R3961 T4552 T4553 arg1Of production,and
R3962 T4553 T4550 arg2Of and,augmenting
R3963 T4554 T4553 arg2Of secretion,and
R3964 T4557 T4555 arg2Of stimulation,upon
R3965 T4557 T4556 arg1Of stimulation,inflammasome
R3966 T4558 T4559 arg1Of Dependency,of
R3967 T4558 T4562 arg1Of Dependency,on
R3968 T4558 T4564 arg1Of Dependency,was
R3969 T4558 T4565 arg2Of Dependency,confirmed
R3970 T4561 T4559 arg2Of responses,of
R3971 T4561 T4560 arg1Of responses,these
R3972 T4563 T4562 arg2Of NF-κB,on
R3973 T4565 T4564 arg2Of confirmed,was
R3974 T4568 T4565 arg1Of inhibition,confirmed
R3975 T4568 T4566 arg2Of inhibition,by
R3976 T4568 T4567 arg1Of inhibition,pharmacologic
R3977 T4568 T4569 arg1Of inhibition,of
R3978 T4568 T4571 arg1Of inhibition,using
R3979 T4570 T4569 arg2Of NF-κB,of
R3980 T4574 T4571 arg2Of inhibitor,using
R3981 T4574 T4572 arg1Of inhibitor,the
R3982 T4574 T4573 arg1Of inhibitor,Bay11-7082
R3983 T4574 T4575 arg1Of inhibitor,of
R3984 T4574 T4578 arg1Of inhibitor,","
R3985 T4574 T4579 arg1Of inhibitor,which
R3986 T4574 T4581 arg1Of inhibitor,reduced
R3987 T4577 T4575 arg2Of phosphorylation,of
R3988 T4577 T4576 arg1Of phosphorylation,IκBα
R3989 T4581 T4580 arg1Of reduced,significantly
R3990 T4581 T4615 arg1Of reduced,(
R3991 T4581 T4619 arg1Of reduced,in
R3992 T4583 T4582 arg1Of activation,NF-κB/AP-1
R3993 T4583 T4584 arg1Of activation,(
R3994 T4583 T4589 arg1Of activation,(
R3995 T4583 T4593 arg1Of activation,and
R3996 T4585 T4584 arg2Of P,(
R3997 T4585 T4586 arg1Of P,<
R3998 T4585 T4587 arg1Of P,0.001
R3999 T4588 T4584 arg3Of ),(
R4000 T4591 T4589 arg2Of 6d,(
R4001 T4591 T4590 arg1Of 6d,Fig.
R4002 T4592 T4589 arg3Of ),(
R4003 T4593 T4608 arg1Of and,and
R4004 T4596 T4593 arg2Of production,and
R4005 T4596 T4594 arg1Of production,the
R4006 T4596 T4595 arg1Of production,subsequent
R4007 T4596 T4597 arg1Of production,of
R4008 T4598 T4597 arg2Of IL-8,of
R4009 T4598 T4599 arg1Of IL-8,(
R4010 T4598 T4604 arg1Of IL-8,(
R4011 T4600 T4599 arg2Of P,(
R4012 T4600 T4601 arg1Of P,<
R4013 T4600 T4602 arg1Of P,0.01
R4014 T4603 T4599 arg3Of ),(
R4015 T4606 T4604 arg2Of 6e,(
R4016 T4606 T4605 arg1Of 6e,Fig.
R4017 T4607 T4604 arg3Of ),(
R4018 T4608 T4581 arg2Of and,reduced
R4019 T4609 T4608 arg2Of IL-1β,and
R4020 T4609 T4610 arg1Of IL-1β,(
R4021 T4611 T4610 arg2Of P,(
R4022 T4611 T4612 arg1Of P,<
R4023 T4611 T4613 arg1Of P,0.001
R4024 T4614 T4610 arg3Of ),(
R4025 T4616 T4615 arg2Of Fig.,(
R4026 T4616 T4617 arg1Of Fig.,6f
R4027 T4618 T4615 arg3Of ),(
R4028 T4620 T4619 arg2Of response,in
R4029 T4620 T4621 arg1Of response,to
R4030 T4623 T4624 arg1Of heat-killed,and
R4031 T4624 T4622 arg1Of and,both
R4032 T4625 T4624 arg2Of live,and
R4033 T4626 T4621 arg2Of PAO1,to
R4034 T4626 T4623 arg1Of PAO1,heat-killed
R4035 T4626 T4625 arg1Of PAO1,live
R4036 T4628 T4627 arg1Of results,These
R4037 T4628 T4629 arg1Of results,were
R4038 T4628 T4631 arg2Of results,verified
R4039 T4631 T4629 arg2Of verified,were
R4040 T4631 T4630 arg1Of verified,also
R4041 T4631 T4632 arg1Of verified,in
R4042 T4633 T4634 arg1Of CF,and
R4043 T4635 T4634 arg2Of control,and
R4044 T4636 T4632 arg2Of PBMCs,in
R4045 T4636 T4633 arg1Of PBMCs,CF
R4046 T4636 T4635 arg1Of PBMCs,control
R4047 T4636 T4637 arg1Of PBMCs,for
R4048 T4639 T4637 arg2Of inflammasome,for
R4049 T4639 T4638 arg1Of inflammasome,each
R4050 T4639 T4640 arg2Of inflammasome,examined
R4051 T4640 T4641 arg1Of examined,(
R4052 T4640 T4646 arg1Of examined,(
R4053 T4642 T4641 arg2Of P,(
R4054 T4642 T4643 arg1Of P,<
R4055 T4642 T4644 arg1Of P,0.001
R4056 T4645 T4641 arg3Of ),(
R4057 T4648 T4646 arg2Of 6g–h,(
R4058 T4648 T4647 arg1Of 6g–h,Fig.
R4059 T4649 T4646 arg3Of ),(
R4060 T4652 T4651 arg1Of results,these
R4061 T4652 T4653 arg1Of results,confirm
R4062 T4653 T4650 arg1Of confirm,Overall
R4063 T4655 T4656 arg1Of NF-κB,is
R4064 T4656 T4654 arg1Of is,that
R4065 T4656 T4663 arg1Of is,and
R4066 T4659 T4656 arg2Of modulator,is
R4067 T4659 T4657 arg1Of modulator,an
R4068 T4659 T4658 arg1Of modulator,important
R4069 T4659 T4660 arg1Of modulator,of
R4070 T4662 T4660 arg2Of production,of
R4071 T4662 T4661 arg1Of production,IL-1β
R4072 T4663 T4653 arg2Of and,confirm
R4073 T4664 T4663 arg2Of that,and
R4074 T4666 T4665 arg2Of activation,increased
R4075 T4666 T4667 arg1Of activation,of
R4076 T4666 T4669 arg1Of activation,augments
R4077 T4668 T4667 arg2Of NF-κB,of
R4078 T4669 T4664 arg2Of augments,that
R4079 T4671 T4669 arg2Of production,augments
R4080 T4671 T4670 arg1Of production,inflammasome-mediated
R4081 T4671 T4672 arg1Of production,of
R4082 T4673 T4672 arg2Of IL-1β,of
R4499 T5212 T5213 arg1Of Bay11-7082,inhibits
R4500 T5213 T5216 arg1Of inhibits,in
R4501 T5215 T5213 arg2Of production,inhibits
R4502 T5215 T5214 arg1Of production,pro-IL-1β
R4503 T5217 T5216 arg2Of response,in
R4504 T5217 T5218 arg1Of response,to
R4505 T5220 T5218 arg2Of aeruginosa,to
R4506 T5220 T5219 arg1Of aeruginosa,P.
R4507 T5222 T5221 arg2Of addition,In
R4508 T5222 T5223 arg1Of addition,to
R4509 T5224 T5223 arg2Of inhibition,to
R4510 T5224 T5225 arg1Of inhibition,of
R4511 T5227 T5225 arg2Of activity,of
R4512 T5227 T5226 arg1Of activity,NF-κB
R4513 T5229 T5230 arg1Of Bay11-7082,can
R4514 T5229 T5233 arg1Of Bay11-7082,inhibit
R4515 T5233 T5221 arg1Of inhibit,In
R4516 T5233 T5228 arg1Of inhibit,","
R4517 T5233 T5230 arg2Of inhibit,can
R4518 T5233 T5231 arg1Of inhibit,also
R4519 T5233 T5232 arg1Of inhibit,directly
R4520 T5233 T5237 arg1Of inhibit,[
R4521 T5236 T5233 arg2Of inflammasome,inhibit
R4522 T5236 T5234 arg1Of inflammasome,the
R4523 T5236 T5235 arg1Of inflammasome,NLRP3
R4524 T5238 T5237 arg2Of 38,[
R4525 T5239 T5237 arg3Of ],[
R4526 T5241 T5240 arg1Of validate,To
R4527 T5243 T5241 arg2Of use,validate
R4528 T5243 T5242 arg1Of use,its
R4529 T5243 T5244 arg1Of use,in
R4530 T5246 T5244 arg2Of study,in
R4531 T5246 T5245 arg1Of study,this
R4532 T5246 T5247 arg1Of study,as
R4533 T5250 T5247 arg2Of inhibitor,as
R4534 T5250 T5248 arg1Of inhibitor,an
R4535 T5250 T5249 arg1Of inhibitor,NF-κB
R4536 T5253 T5252 arg1Of blots,western
R4537 T5253 T5254 arg1Of blots,for
R4538 T5253 T5256 arg1Of blots,were
R4539 T5253 T5257 arg2Of blots,performed
R4540 T5255 T5254 arg2Of pro-IL-1β,for
R4541 T5257 T5240 modOf performed,To
R4542 T5257 T5251 arg1Of performed,","
R4543 T5257 T5256 arg2Of performed,were
R4544 T5257 T5258 arg1Of performed,alongside
R4545 T5257 T5265 arg1Of performed,in
R4546 T5257 T5269 arg1Of performed,at
R4547 T5257 T5272 arg1Of performed,after
R4548 T5259 T5258 arg2Of inhibition,alongside
R4549 T5259 T5260 arg1Of inhibition,of
R4550 T5259 T5263 arg1Of inhibition,by
R4551 T5262 T5260 arg2Of activity,of
R4552 T5262 T5261 arg1Of activity,CFTR
R4553 T5264 T5263 arg2Of CFTRinh172,by
R4554 T5265 T5267 arg1Of in,to
R4555 T5266 T5265 arg2Of response,in
R4556 T5268 T5265 arg3Of PAO1,in
R4557 T5271 T5269 arg2Of hours,at
R4558 T5271 T5270 arg1Of hours,4
R4559 T5273 T5272 arg2Of stimulation,after
R4560 T5273 T5274 arg1Of stimulation,(
R4561 T5276 T5274 arg2Of 7a,(
R4562 T5276 T5275 arg1Of 7a,Fig.
R4563 T5277 T5274 arg3Of ),(
R4564 T5279 T5278 arg1Of results,Our
R4565 T5279 T5280 arg1Of results,indicate
R4566 T5282 T5283 arg1Of Bay11-7082,prevents
R4567 T5283 T5280 arg2Of prevents,indicate
R4568 T5283 T5281 arg1Of prevents,that
R4569 T5283 T5287 arg1Of prevents,whereas
R4570 T5284 T5283 arg2Of production,prevents
R4571 T5284 T5285 arg1Of production,of
R4572 T5286 T5285 arg2Of pro-IL-1β,of
R4573 T5288 T5289 arg1Of CFTRinh172,does
R4574 T5288 T5291 arg1Of CFTRinh172,seem
R4575 T5288 T5293 arg1Of CFTRinh172,affect
R4576 T5291 T5287 arg2Of seem,whereas
R4577 T5291 T5289 arg2Of seem,does
R4578 T5291 T5290 arg1Of seem,not
R4579 T5293 T5291 arg2Of affect,seem
R4580 T5293 T5292 arg1Of affect,to
R4581 T5294 T5293 arg2Of it,affect
R4582 T5295 T5296 arg1Of This,was
R4583 T5295 T5298 arg2Of This,corroborated
R4584 T5298 T5296 arg2Of corroborated,was
R4585 T5298 T5297 arg1Of corroborated,further
R4586 T5298 T5312 arg1Of corroborated,and
R4587 T5302 T5303 modOf Bay11-7082,to
R4588 T5302 T5309 arg1Of Bay11-7082,","
R4589 T5304 T5303 arg1Of inhibit,to
R4590 T5304 T5307 arg1Of inhibit,at
R4591 T5306 T5304 arg2Of degradation,inhibit
R4592 T5306 T5305 arg1Of degradation,IκBα
R4593 T5308 T5307 arg2Of 0.5,at
R4594 T5309 T5298 arg1Of ",",corroborated
R4595 T5309 T5299 arg2Of ",",by
R4596 T5309 T5300 arg1Of ",",the
R4597 T5309 T5301 arg1Of ",",ability
R4598 T5310 T5309 arg2Of 1,","
R4599 T5312 T5311 arg1Of and,","
R4600 T5314 T5313 arg1Of hours,1.5
R4601 T5314 T5315 arg1Of hours,post
R4602 T5315 T5312 arg2Of post,and
R4603 T5317 T5315 arg2Of stimulation,post
R4604 T5317 T5316 arg1Of stimulation,PAO1
R4605 T5317 T5318 arg1Of stimulation,(
R4606 T5320 T5318 arg2Of 7b,(
R4607 T5320 T5319 arg1Of 7b,Fig.
R4608 T5321 T5318 arg3Of ),(
R4753 T5503 T5504 arg1Of Disruption,of
R4754 T5503 T5507 arg1Of Disruption,does
R4755 T5503 T5509 arg1Of Disruption,increase
R4756 T5506 T5504 arg2Of activity,of
R4757 T5506 T5505 arg1Of activity,CFTR
R4758 T5509 T5507 arg2Of increase,does
R4759 T5509 T5508 arg1Of increase,not
R4760 T5511 T5509 arg2Of production,increase
R4761 T5511 T5510 arg1Of production,IL-1β
R4762 T5511 T5512 arg1Of production,in
R4763 T5513 T5514 arg1Of PBMCs,and
R4764 T5514 T5512 arg2Of and,in
R4765 T5516 T5514 arg2Of cells,and
R4766 T5516 T5515 arg1Of cells,THP-1
R4767 T5519 T5517 arg1Of study,A
R4768 T5519 T5518 arg1Of study,previous
R4769 T5519 T5520 arg1Of study,has
R4770 T5519 T5521 arg1Of study,indicated
R4771 T5521 T5520 arg2Of indicated,has
R4772 T5523 T5521 arg2Of role,indicated
R4773 T5523 T5522 arg1Of role,a
R4774 T5523 T5524 arg1Of role,for
R4775 T5523 T5528 arg1Of role,in
R4776 T5527 T5524 arg2Of concentration,for
R4777 T5527 T5525 arg1Of concentration,chloride
R4778 T5527 T5526 arg1Of concentration,ion
R4779 T5529 T5528 arg2Of suppression,in
R4780 T5529 T5530 arg1Of suppression,of
R4781 T5533 T5530 arg2Of activation,of
R4782 T5533 T5531 arg1Of activation,NLRP3
R4783 T5533 T5532 arg1Of activation,inflammasome
R4784 T5533 T5534 arg1Of activation,[
R4785 T5535 T5534 arg2Of 34,[
R4786 T5536 T5534 arg3Of ],[
R4787 T5538 T5537 arg1Of determine,To
R4788 T5541 T5540 arg1Of dysfunction,CFTR
R4789 T5541 T5542 arg1Of dysfunction,alters
R4790 T5542 T5538 arg2Of alters,determine
R4791 T5542 T5539 arg1Of alters,whether
R4792 T5544 T5542 arg2Of production,alters
R4793 T5544 T5543 arg1Of production,IL-1β
R4794 T5547 T5546 arg1Of cells,THP-1
R4795 T5547 T5548 arg1Of cells,and
R4796 T5548 T5556 arg1Of and,were
R4797 T5548 T5557 arg2Of and,treated
R4798 T5549 T5548 arg2Of PBMCs,and
R4799 T5549 T5550 arg1Of PBMCs,from
R4800 T5552 T5551 arg1Of patients,CF
R4801 T5552 T5553 arg1Of patients,and
R4802 T5553 T5550 arg2Of and,from
R4803 T5555 T5553 arg2Of controls,and
R4804 T5555 T5554 arg1Of controls,healthy
R4805 T5557 T5537 modOf treated,To
R4806 T5557 T5545 arg1Of treated,","
R4807 T5557 T5556 arg2Of treated,were
R4808 T5557 T5558 arg1Of treated,with
R4809 T5561 T5558 arg2Of inhibitor,with
R4810 T5561 T5559 arg1Of inhibitor,the
R4811 T5561 T5560 arg1Of inhibitor,CFTR
R4812 T5561 T5562 arg1Of inhibitor,","
R4813 T5563 T5562 arg2Of CFTRinh172,","
R4814 T5563 T5564 arg1Of CFTRinh172,","
R4815 T5563 T5565 arg1Of CFTRinh172,prior
R4816 T5565 T5566 arg1Of prior,to
R4817 T5567 T5566 arg2Of simulation,to
R4818 T5567 T5568 arg1Of simulation,with
R4819 T5571 T5568 arg2Of aeruginosa,with
R4820 T5571 T5569 arg1Of aeruginosa,live
R4821 T5571 T5570 arg1Of aeruginosa,P.
R4822 T5572 T5573 arg1Of Treatment,with
R4823 T5572 T5575 arg1Of Treatment,did
R4824 T5572 T5577 arg1Of Treatment,alter
R4825 T5574 T5573 arg2Of CFTRinh172,with
R4826 T5577 T5575 arg2Of alter,did
R4827 T5577 T5576 arg1Of alter,not
R4828 T5578 T5579 arg1Of IL-1β,or
R4829 T5580 T5579 arg2Of IL-8,or
R4830 T5581 T5578 arg1Of production,IL-1β
R4831 T5581 T5580 arg1Of production,IL-8
R4832 T5581 T5582 arg1Of production,in
R4833 T5581 T5585 arg1Of production,or
R4834 T5584 T5582 arg2Of subjects,in
R4835 T5584 T5583 arg1Of subjects,control
R4836 T5585 T5577 arg2Of or,alter
R4837 T5587 T5585 arg2Of patients,or
R4838 T5587 T5586 arg1Of patients,CF
R4839 T5587 T5588 arg1Of patients,(
R4840 T5590 T5588 arg2Of 8a–b,(
R4841 T5590 T5589 arg1Of 8a–b,Fig.
R4842 T5591 T5588 arg3Of ),(
R4843 T5595 T5594 arg1Of production,IL-1β
R4844 T5595 T5596 arg1Of production,was
R4845 T5595 T5598 arg1Of production,different
R4846 T5596 T5592 arg1Of was,Similarly
R4847 T5596 T5593 arg1Of was,","
R4848 T5596 T5597 arg1Of was,not
R4849 T5598 T5596 arg2Of different,was
R4850 T5598 T5599 arg1Of different,in
R4851 T5601 T5600 arg1Of macrophages,monocyte-derived
R4852 T5601 T5602 arg1Of macrophages,or
R4853 T5602 T5599 arg2Of or,in
R4854 T5602 T5606 arg2Of or,treated
R4855 T5605 T5602 arg2Of cells,or
R4856 T5605 T5603 arg1Of cells,THP-1
R4857 T5605 T5604 arg1Of cells,reporter
R4858 T5606 T5607 arg1Of treated,with
R4859 T5608 T5607 arg2Of CFTRinh172,with
R4860 T5608 T5609 arg1Of CFTRinh172,(
R4861 T5611 T5609 arg2Of 8c–d,(
R4862 T5611 T5610 arg1Of 8c–d,Fig.
R4863 T5612 T5609 arg3Of ),(
R4864 T5613 T5614 arg1Of IL-8,(
R4865 T5613 T5618 arg1Of IL-8,and
R4866 T5616 T5614 arg2Of 8e,(
R4867 T5616 T5615 arg1Of 8e,Fig.
R4868 T5617 T5614 arg3Of ),(
R4869 T5618 T5625 arg1Of and,were
R4870 T5618 T5627 arg1Of and,unchanged
R4871 T5620 T5618 arg2Of activity,and
R4872 T5620 T5619 arg1Of activity,NF-κB
R4873 T5620 T5621 arg1Of activity,(
R4874 T5623 T5621 arg2Of 8f,(
R4875 T5623 T5622 arg1Of 8f,Fig.
R4876 T5624 T5621 arg3Of ),(
R4877 T5625 T5626 arg1Of were,also
R4878 T5627 T5625 arg2Of unchanged,were
R4879 T5627 T5628 arg1Of unchanged,in
R4880 T5632 T5628 arg2Of cells,in
R4881 T5632 T5629 arg1Of cells,CFTRinh172-treated
R4882 T5632 T5630 arg1Of cells,THP-1
R4883 T5632 T5631 arg1Of cells,reporter
R5169 T6078 T6079 arg1Of Levels,of
R5170 T6078 T6081 arg1Of Levels,are
R5171 T6078 T6082 arg2Of Levels,increased
R5172 T6080 T6079 arg2Of IL-1β,of
R5173 T6082 T6081 arg2Of increased,are
R5174 T6082 T6083 arg1Of increased,in
R5175 T6082 T6089 arg1Of increased,but
R5176 T6085 T6083 arg2Of BALF,in
R5177 T6085 T6084 arg1Of BALF,the
R5178 T6085 T6086 arg1Of BALF,of
R5179 T6088 T6086 arg2Of patients,of
R5180 T6088 T6087 arg1Of patients,CF
R5181 T6092 T6090 arg1Of source,the
R5182 T6092 T6091 arg1Of source,cellular
R5183 T6092 T6093 arg1Of source,of
R5184 T6092 T6099 arg1Of source,in
R5185 T6092 T6106 arg1Of source,are
R5186 T6092 T6108 arg1Of source,unclear
R5187 T6095 T6094 arg1Of cytokine,this
R5188 T6095 T6096 arg1Of cytokine,and
R5189 T6096 T6093 arg2Of and,of
R5190 T6098 T6096 arg2Of production,and
R5191 T6098 T6097 arg1Of production,its
R5192 T6101 T6099 arg2Of context,in
R5193 T6101 T6100 arg1Of context,the
R5194 T6101 T6102 arg1Of context,of
R5195 T6105 T6102 arg2Of activation,of
R5196 T6105 T6103 arg2Of activation,targeted
R5197 T6105 T6104 arg1Of activation,inflammasome
R5198 T6106 T6089 arg2Of are,but
R5199 T6106 T6107 arg1Of are,still
R5200 T6108 T6106 arg2Of unclear,are
R5201 T6109 T6111 arg1Of We,studied
R5202 T6111 T6110 arg1Of studied,first
R5203 T6111 T6116 arg1Of studied,to
R5204 T6111 T6119 arg1Of studied,in
R5205 T6114 T6111 arg2Of cells,studied
R5206 T6114 T6112 arg1Of cells,airway
R5207 T6114 T6113 arg1Of cells,epithelial
R5208 T6116 T6115 arg1Of to,due
R5209 T6118 T6116 arg2Of role,to
R5210 T6118 T6117 arg1Of role,their
R5211 T6121 T6120 arg1Of function,barrier
R5212 T6121 T6122 arg1Of function,","
R5213 T6122 T6127 arg1Of ",",and
R5214 T6123 T6122 arg2Of proximity,","
R5215 T6123 T6124 arg1Of proximity,to
R5216 T6125 T6124 arg2Of infection,to
R5217 T6127 T6119 arg2Of and,in
R5218 T6127 T6126 arg1Of and,","
R5219 T6128 T6127 arg2Of ability,and
R5220 T6128 T6129 modOf ability,to
R5221 T6130 T6129 arg1Of produce,to
R5222 T6132 T6130 arg2Of levels,produce
R5223 T6132 T6131 arg1Of levels,high
R5224 T6132 T6133 arg1Of levels,of
R5225 T6135 T6133 arg2Of cytokines,of
R5226 T6135 T6134 arg1Of cytokines,pro-inflammatory
R5227 T6138 T6139 arg1Of we,found
R5228 T6139 T6136 arg1Of found,However
R5229 T6139 T6137 arg1Of found,","
R5230 T6143 T6141 arg1Of cells,bronchial
R5231 T6143 T6142 arg1Of cells,epithelial
R5232 T6143 T6144 arg1Of cells,do
R5233 T6143 T6146 arg1Of cells,produce
R5234 T6143 T6152 arg1Of cells,do
R5235 T6143 T6154 arg1Of cells,show
R5236 T6146 T6144 arg2Of produce,do
R5237 T6146 T6145 arg1Of produce,not
R5238 T6146 T6151 arg1Of produce,and
R5239 T6148 T6146 arg2Of amounts,produce
R5240 T6148 T6147 arg1Of amounts,significant
R5241 T6148 T6149 arg1Of amounts,of
R5242 T6150 T6149 arg2Of IL-1β,of
R5243 T6151 T6139 arg2Of and,found
R5244 T6151 T6140 arg1Of and,that
R5245 T6151 T6167 arg1Of and,","
R5246 T6151 T6168 arg1Of and,in
R5247 T6154 T6151 arg2Of show,and
R5248 T6154 T6152 arg2Of show,do
R5249 T6154 T6153 arg1Of show,not
R5250 T6157 T6154 arg2Of increase,show
R5251 T6157 T6155 arg1Of increase,a
R5252 T6157 T6156 arg1Of increase,significant
R5253 T6157 T6158 arg1Of increase,in
R5254 T6157 T6161 arg1Of increase,in
R5255 T6160 T6158 arg2Of activation,in
R5256 T6160 T6159 arg1Of activation,caspase-1
R5257 T6162 T6161 arg2Of response,in
R5258 T6162 T6163 arg1Of response,to
R5259 T6164 T6165 arg1Of PAO1,and
R5260 T6165 T6163 arg2Of and,to
R5261 T6166 T6165 arg2Of LPS+ATP,and
R5262 T6169 T6168 arg2Of comparison,in
R5263 T6169 T6170 arg1Of comparison,to
R5264 T6173 T6170 arg2Of cells,to
R5265 T6173 T6171 arg1Of cells,hematopoeitic
R5266 T6173 T6172 arg1Of cells,mononuclear
R5267 T6175 T6174 arg1Of cells,Hematopoeitically-derived
R5268 T6175 T6176 arg1Of cells,","
R5269 T6175 T6178 arg1Of cells,as
R5270 T6175 T6183 arg1Of cells,appear
R5271 T6175 T6185 arg1Of cells,be
R5272 T6178 T6177 arg1Of as,such
R5273 T6179 T6180 arg1Of monocytes,and
R5274 T6180 T6178 arg2Of and,as
R5275 T6181 T6180 arg2Of macrophages,and
R5276 T6183 T6182 arg1Of appear,","
R5277 T6185 T6183 arg2Of be,appear
R5278 T6185 T6184 arg1Of be,to
R5279 T6188 T6185 arg2Of source,be
R5280 T6188 T6186 arg1Of source,a
R5281 T6188 T6187 arg1Of source,principal
R5282 T6188 T6189 arg1Of source,of
R5283 T6190 T6189 arg2Of IL-1β,of
R5284 T6191 T6192 arg1Of CFTR,is
R5285 T6191 T6193 arg2Of CFTR,expressed
R5286 T6193 T6192 arg2Of expressed,is
R5287 T6193 T6194 arg1Of expressed,in
R5288 T6193 T6207 arg1Of expressed,in
R5289 T6193 T6219 arg1Of expressed,","
R5290 T6193 T6220 arg1Of expressed,[
R5291 T6193 T6224 arg1Of expressed,and
R5292 T6194 T6206 arg1Of in,as
R5293 T6196 T6194 arg2Of macrophages,in
R5294 T6196 T6195 arg1Of macrophages,alveolar
R5295 T6196 T6197 arg1Of macrophages,[
R5296 T6196 T6200 arg1Of macrophages,","
R5297 T6196 T6201 arg1Of macrophages,[
R5298 T6198 T6197 arg2Of 32,[
R5299 T6199 T6197 arg3Of ],[
R5300 T6202 T6201 arg2Of 39,[
R5301 T6203 T6201 arg3Of ],[
R5302 T6206 T6204 arg1Of as,as
R5303 T6206 T6205 arg1Of as,well
R5304 T6207 T6206 arg2Of in,as
R5305 T6208 T6207 arg2Of PBMCs,in
R5306 T6208 T6209 arg1Of PBMCs,at
R5307 T6212 T6213 arg1Of mRNA,and
R5308 T6214 T6213 arg2Of protein,and
R5309 T6215 T6209 arg2Of level,at
R5310 T6215 T6210 arg1Of level,both
R5311 T6215 T6211 arg1Of level,the
R5312 T6215 T6212 arg1Of level,mRNA
R5313 T6215 T6214 arg1Of level,protein
R5314 T6215 T6216 arg1Of level,[
R5315 T6217 T6216 arg2Of 40,[
R5316 T6218 T6216 arg3Of ],[
R5317 T6221 T6220 arg2Of 41,[
R5318 T6222 T6220 arg3Of ],[
R5319 T6224 T6223 arg1Of and,","
R5320 T6226 T6225 arg1Of loss,its
R5321 T6226 T6227 arg1Of loss,is
R5322 T6226 T6229 arg2Of loss,associated
R5323 T6229 T6224 arg2Of associated,and
R5324 T6229 T6227 arg2Of associated,is
R5325 T6229 T6228 arg1Of associated,frequently
R5326 T6229 T6230 arg1Of associated,with
R5327 T6234 T6230 arg2Of phenotype,with
R5328 T6234 T6231 arg1Of phenotype,an
R5329 T6234 T6232 arg1Of phenotype,augmented
R5330 T6234 T6233 arg1Of phenotype,inflammatory
R5331 T6237 T6235 arg2Of findings,Despite
R5332 T6237 T6236 arg1Of findings,our
R5333 T6237 T6238 arg1Of findings,indicating
R5334 T6242 T6240 arg1Of cells,bronchial
R5335 T6242 T6241 arg1Of cells,epithelial
R5336 T6242 T6244 arg1Of cells,grown
R5337 T6242 T6247 arg1Of cells,are
R5338 T6242 T6248 arg1Of cells,unlikely
R5339 T6242 T6250 arg1Of cells,be
R5340 T6242 T6252 arg2Of cells,involved
R5341 T6244 T6243 arg2Of grown,when
R5342 T6244 T6246 arg1Of grown,vitro
R5343 T6246 T6245 arg1Of vitro,in
R5344 T6247 T6238 arg2Of are,indicating
R5345 T6247 T6239 arg1Of are,that
R5346 T6247 T6243 arg1Of are,when
R5347 T6248 T6247 arg2Of unlikely,are
R5348 T6252 T6248 arg2Of involved,unlikely
R5349 T6252 T6249 arg1Of involved,to
R5350 T6252 T6250 arg2Of involved,be
R5351 T6252 T6251 arg1Of involved,significantly
R5352 T6252 T6253 arg1Of involved,in
R5353 T6256 T6253 arg2Of production,in
R5354 T6256 T6254 arg1Of production,the
R5355 T6256 T6255 arg1Of production,direct
R5356 T6256 T6257 arg1Of production,of
R5357 T6258 T6257 arg2Of IL-1β,of
R5358 T6258 T6259 arg1Of IL-1β,(
R5359 T6261 T6259 arg2Of 2a–d,(
R5360 T6261 T6260 arg1Of 2a–d,Fig.
R5361 T6262 T6259 arg3Of ),(
R5362 T6264 T6265 arg1Of others,have
R5363 T6264 T6266 arg1Of others,shown
R5364 T6266 T6235 arg1Of shown,Despite
R5365 T6266 T6263 arg1Of shown,","
R5366 T6266 T6265 arg2Of shown,have
R5367 T6268 T6266 arg2Of capacity,shown
R5368 T6268 T6267 arg1Of capacity,their
R5369 T6268 T6269 modOf capacity,to
R5370 T6268 T6276 modOf capacity,to
R5371 T6270 T6269 arg1Of respond,to
R5372 T6270 T6271 arg1Of respond,to
R5373 T6270 T6275 arg1Of respond,and
R5374 T6274 T6271 arg2Of IL-1β,to
R5375 T6274 T6272 arg1Of IL-1β,alveolar
R5376 T6274 T6273 arg1Of IL-1β,macrophage-derived
R5377 T6277 T6275 arg2Of amplify,and
R5378 T6277 T6276 arg1Of amplify,to
R5379 T6277 T6281 arg1Of amplify,through
R5380 T6277 T6292 arg1Of amplify,[
R5381 T6280 T6277 arg2Of response,amplify
R5382 T6280 T6278 arg1Of response,the
R5383 T6280 T6279 arg1Of response,inflammatory
R5384 T6283 T6284 arg1Of induction,of
R5385 T6283 T6286 arg1Of induction,and
R5386 T6285 T6284 arg2Of chemokines,of
R5387 T6286 T6281 arg2Of and,through
R5388 T6286 T6282 arg1Of and,the
R5389 T6287 T6286 arg2Of recruitment,and
R5390 T6287 T6288 arg1Of recruitment,of
R5391 T6291 T6288 arg2Of cells,of
R5392 T6291 T6289 arg1Of cells,inflammatory
R5393 T6291 T6290 arg1Of cells,effector
R5394 T6293 T6292 arg2Of 42,[
R5395 T6294 T6292 arg3Of ],[
R5396 T6296 T6295 arg1Of interaction,This
R5397 T6296 T6297 arg1Of interaction,may
R5398 T6296 T6298 arg1Of interaction,constitute
R5399 T6298 T6297 arg2Of constitute,may
R5400 T6298 T6306 arg1Of constitute,and
R5401 T6301 T6298 arg2Of component,constitute
R5402 T6301 T6299 arg1Of component,a
R5403 T6301 T6300 arg1Of component,critical
R5404 T6301 T6302 arg1Of component,to
R5405 T6305 T6302 arg2Of defense,to
R5406 T6305 T6303 arg1Of defense,effective
R5407 T6305 T6304 arg1Of defense,host
R5408 T6307 T6317 arg1Of diminishing,may
R5409 T6307 T6318 arg1Of diminishing,leave
R5410 T6309 T6307 arg2Of capacity,diminishing
R5411 T6309 T6308 arg1Of capacity,the
R5412 T6309 T6310 arg1Of capacity,of
R5413 T6309 T6313 modOf capacity,to
R5414 T6312 T6310 arg2Of cells,of
R5415 T6312 T6311 arg1Of cells,host
R5416 T6314 T6313 arg1Of respond,to
R5417 T6314 T6315 arg1Of respond,to
R5418 T6316 T6315 arg2Of IL-1β,to
R5419 T6318 T6306 arg2Of leave,and
R5420 T6318 T6317 arg2Of leave,may
R5421 T6320 T6318 arg2Of host,leave
R5422 T6320 T6319 arg1Of host,the
R5423 T6320 T6321 arg1Of host,susceptible
R5424 T6321 T6318 arg3Of susceptible,leave
R5425 T6321 T6322 arg1Of susceptible,to
R5426 T6323 T6322 arg2Of infections,to
R5427 T6323 T6324 arg1Of infections,by
R5428 T6325 T6324 arg2Of pathogens,by
R5429 T6325 T6327 arg1Of pathogens,as
R5430 T6327 T6326 arg1Of as,such
R5431 T6329 T6327 arg2Of aeruginosa,as
R5432 T6329 T6328 arg1Of aeruginosa,P.
R5433 T6329 T6330 arg1Of aeruginosa,[
R5434 T6331 T6330 arg2Of 43,[
R5435 T6332 T6330 arg3Of ],[
R5436 T6335 T6336 arg1Of overproduction,of
R5437 T6335 T6338 arg1Of overproduction,can
R5438 T6335 T6340 arg1Of overproduction,play
R5439 T6335 T6349 arg1Of overproduction,cause
R5440 T6337 T6336 arg2Of IL-1β,of
R5441 T6340 T6338 arg2Of play,can
R5442 T6340 T6339 arg1Of play,also
R5443 T6340 T6344 arg1Of play,in
R5444 T6340 T6348 arg1Of play,and
R5445 T6343 T6340 arg2Of role,play
R5446 T6343 T6341 arg1Of role,a
R5447 T6343 T6342 arg1Of role,key
R5448 T6347 T6344 arg2Of responses,in
R5449 T6347 T6345 arg1Of responses,chronic
R5450 T6347 T6346 arg1Of responses,inflammatory
R5451 T6348 T6333 arg1Of and,Conversely
R5452 T6348 T6334 arg1Of and,","
R5453 T6349 T6348 arg2Of cause,and
R5454 T6349 T6358 arg1Of cause,","
R5455 T6349 T6359 arg1Of cause,[
R5456 T6350 T6349 arg2Of damage,cause
R5457 T6350 T6351 arg1Of damage,to
R5458 T6354 T6351 arg2Of parenchyma,to
R5459 T6354 T6352 arg1Of parenchyma,the
R5460 T6354 T6353 arg1Of parenchyma,lung
R5461 T6354 T6355 arg1Of parenchyma,[
R5462 T6356 T6355 arg2Of 44,[
R5463 T6357 T6355 arg3Of ],[
R5464 T6360 T6359 arg2Of 45,[
R5465 T6361 T6359 arg3Of ],[
R5466 T6363 T6364 arg1Of we,hypothesized
R5467 T6364 T6362 arg2Of hypothesized,Although
R5468 T6367 T6366 arg1Of cells,CF
R5469 T6367 T6368 arg1Of cells,would
R5470 T6367 T6369 arg1Of cells,secrete
R5471 T6369 T6364 arg2Of secrete,hypothesized
R5472 T6369 T6365 arg1Of secrete,that
R5473 T6369 T6368 arg2Of secrete,would
R5474 T6371 T6369 arg2Of amounts,secrete
R5475 T6371 T6370 arg2Of amounts,increased
R5476 T6371 T6372 arg1Of amounts,of
R5477 T6373 T6372 arg2Of IL-1β,of
R5478 T6375 T6376 arg1Of we,found
R5479 T6376 T6362 arg1Of found,Although
R5480 T6376 T6374 arg1Of found,","
R5481 T6379 T6378 arg1Of production,IL-1β
R5482 T6379 T6380 arg1Of production,in
R5483 T6379 T6383 arg1Of production,was
R5484 T6379 T6385 arg2Of production,increased
R5485 T6382 T6380 arg2Of PBMCs,in
R5486 T6382 T6381 arg1Of PBMCs,CF
R5487 T6385 T6376 arg2Of increased,found
R5488 T6385 T6377 arg1Of increased,that
R5489 T6385 T6383 arg2Of increased,was
R5490 T6385 T6384 arg1Of increased,not
R5491 T6385 T6386 arg1Of increased,upon
R5492 T6385 T6389 arg1Of increased,as
R5493 T6388 T6386 arg2Of stimulation,upon
R5494 T6388 T6387 arg1Of stimulation,inflammasome
R5495 T6390 T6389 arg2Of compared,as
R5496 T6390 T6391 arg1Of compared,to
R5497 T6392 T6391 arg2Of controls,to
R5498 T6392 T6393 arg1Of controls,(
R5499 T6395 T6393 arg2Of 5a–b,(
R5500 T6395 T6394 arg1Of 5a–b,Fig.
R5501 T6396 T6393 arg3Of ),(
R5502 T6397 T6398 arg1Of This,was
R5503 T6397 T6399 arg1Of This,in
R5504 T6399 T6398 arg2Of in,was
R5505 T6400 T6399 arg2Of contrast,in
R5506 T6400 T6401 arg1Of contrast,to
R5507 T6404 T6401 arg2Of study,to
R5508 T6404 T6402 arg1Of study,a
R5509 T6404 T6403 arg1Of study,previous
R5510 T6404 T6405 arg1Of study,from
R5511 T6404 T6408 arg1Of study,","
R5512 T6404 T6409 arg1Of study,which
R5513 T6404 T6410 arg1Of study,showed
R5514 T6407 T6405 arg2Of group,from
R5515 T6407 T6406 arg1Of group,our
R5516 T6410 T6417 arg1Of showed,in
R5517 T6410 T6424 arg1Of showed,","
R5518 T6410 T6425 arg1Of showed,although
R5519 T6413 T6410 arg2Of production,showed
R5520 T6413 T6411 arg2Of production,increased
R5521 T6413 T6412 arg1Of production,IL-1β
R5522 T6413 T6414 arg1Of production,by
R5523 T6416 T6414 arg2Of PBMCs,by
R5524 T6416 T6415 arg1Of PBMCs,CF
R5525 T6418 T6417 arg2Of response,in
R5526 T6418 T6419 arg1Of response,to
R5527 T6420 T6419 arg2Of LPS,to
R5528 T6420 T6421 arg1Of LPS,[
R5529 T6422 T6421 arg2Of 46,[
R5530 T6423 T6421 arg3Of ],[
R5531 T6427 T6426 arg1Of difference,this
R5532 T6427 T6428 arg1Of difference,may
R5533 T6427 T6429 arg1Of difference,be
R5534 T6427 T6430 arg2Of difference,accounted
R5535 T6430 T6425 arg2Of accounted,although
R5536 T6430 T6428 arg2Of accounted,may
R5537 T6430 T6429 arg2Of accounted,be
R5538 T6430 T6431 arg1Of accounted,for
R5539 T6430 T6432 arg1Of accounted,by
R5540 T6430 T6435 arg1Of accounted,in
R5541 T6434 T6432 arg2Of issues,by
R5542 T6434 T6433 arg1Of issues,technical
R5543 T6437 T6438 arg1Of time,and
R5544 T6438 T6435 arg2Of and,in
R5545 T6438 T6436 arg1Of and,stimulation
R5546 T6439 T6438 arg2Of dose,and
R5547 T6442 T6455 arg1Of IL-1β,would
R5548 T6442 T6456 arg1Of IL-1β,be
R5549 T6442 T6457 arg2Of IL-1β,anticipated
R5550 T6443 T6444 arg1Of production,was
R5551 T6443 T6446 arg2Of production,increased
R5552 T6446 T6444 arg2Of increased,was
R5553 T6446 T6445 arg1Of increased,not
R5554 T6446 T6447 arg1Of increased,in
R5555 T6449 T6447 arg2Of PBMCs,in
R5556 T6449 T6448 arg1Of PBMCs,CF
R5557 T6449 T6450 arg1Of PBMCs,with
R5558 T6452 T6450 arg2Of stimulation,with
R5559 T6452 T6451 arg1Of stimulation,inflammasome
R5560 T6452 T6453 arg1Of stimulation,alone
R5561 T6457 T6440 arg1Of anticipated,Moreover
R5562 T6457 T6441 arg1Of anticipated,","
R5563 T6457 T6444 modOf anticipated,was
R5564 T6457 T6454 arg1Of anticipated,as
R5565 T6457 T6455 arg2Of anticipated,would
R5566 T6457 T6456 arg2Of anticipated,be
R5567 T6457 T6458 arg1Of anticipated,if
R5568 T6459 T6460 arg1Of there,were
R5569 T6460 T6458 arg2Of were,if
R5570 T6462 T6460 arg2Of levels,were
R5571 T6462 T6461 arg1Of levels,basal
R5572 T6462 T6463 arg1Of levels,of
R5573 T6465 T6463 arg2Of activation,of
R5574 T6465 T6464 arg1Of activation,NF-κB
R5575 T6466 T6467 arg1Of Cells,deficient
R5576 T6466 T6470 arg1Of Cells,are
R5577 T6466 T6471 arg2Of Cells,thought
R5578 T6466 T6473 arg1Of Cells,exhibit
R5579 T6467 T6468 arg1Of deficient,in
R5580 T6469 T6468 arg2Of CFTR,in
R5581 T6471 T6470 arg2Of thought,are
R5582 T6473 T6471 arg3Of exhibit,thought
R5583 T6473 T6472 arg1Of exhibit,to
R5584 T6477 T6473 arg2Of level,exhibit
R5585 T6477 T6474 arg1Of level,an
R5586 T6477 T6475 arg2Of level,increased
R5587 T6477 T6476 arg1Of level,basal
R5588 T6477 T6478 arg1Of level,of
R5589 T6477 T6481 arg1Of level,","
R5590 T6477 T6482 arg1Of level,which
R5591 T6477 T6483 arg1Of level,leads
R5592 T6480 T6478 arg2Of activity,of
R5593 T6480 T6479 arg1Of activity,NF-κB
R5594 T6483 T6484 arg1Of leads,to
R5595 T6488 T6484 arg2Of production,to
R5596 T6488 T6485 arg2Of production,increased
R5597 T6488 T6486 arg1Of production,pro-inflammatory
R5598 T6488 T6487 arg1Of production,cytokine
R5599 T6488 T6489 arg1Of production,including
R5600 T6492 T6489 arg2Of availability,including
R5601 T6492 T6490 arg1Of availability,an
R5602 T6492 T6491 arg2Of availability,increased
R5603 T6492 T6493 arg1Of availability,of
R5604 T6492 T6495 arg1Of availability,for
R5605 T6494 T6493 arg2Of pro-IL-1β,of
R5606 T6496 T6497 arg1Of cleavage,and
R5607 T6497 T6495 arg2Of and,for
R5608 T6498 T6497 arg2Of secretion,and
R5609 T6500 T6499 arg1Of amplification,This
R5610 T6500 T6501 arg1Of amplification,of
R5611 T6500 T6504 arg1Of amplification,was
R5612 T6500 T6505 arg2Of amplification,shown
R5613 T6503 T6501 arg2Of secretion,of
R5614 T6503 T6502 arg1Of secretion,IL-1β
R5615 T6505 T6504 arg2Of shown,was
R5616 T6505 T6506 arg1Of shown,by
R5617 T6507 T6506 arg2Of priming,by
R5618 T6509 T6508 arg1Of monocytes,THP-1
R5619 T6509 T6510 arg1Of monocytes,and
R5620 T6510 T6507 arg2Of and,priming
R5621 T6510 T6512 arg1Of and,with
R5622 T6511 T6510 arg2Of PBMCs,and
R5623 T6515 T6513 arg1Of aeruginosa,heat-killed
R5624 T6515 T6514 arg1Of aeruginosa,P.
R5625 T6515 T6516 arg1Of aeruginosa,or
R5626 T6516 T6512 arg2Of or,with
R5627 T6517 T6516 arg2Of LPS,or
R5628 T6517 T6518 arg1Of LPS,prior
R5629 T6518 T6519 arg1Of prior,to
R5630 T6520 T6519 arg2Of stimulation,to
R5631 T6520 T6521 arg1Of stimulation,with
R5632 T6524 T6521 arg2Of aeruginosa,with
R5633 T6524 T6522 arg1Of aeruginosa,live
R5634 T6524 T6523 arg1Of aeruginosa,P.
R5635 T6527 T6526 arg1Of increased,dramatically
R5636 T6529 T6525 arg1Of secretion,This
R5637 T6529 T6527 arg1Of secretion,increased
R5638 T6529 T6528 arg1Of secretion,IL-1β
R5639 T6529 T6530 arg1Of secretion,over
R5640 T6531 T6530 arg2Of stimulation,over
R5641 T6531 T6532 arg1Of stimulation,with
R5642 T6535 T6532 arg2Of aeruginosa,with
R5643 T6535 T6533 arg1Of aeruginosa,live
R5644 T6535 T6534 arg1Of aeruginosa,P.
R5645 T6535 T6536 arg1Of aeruginosa,without
R5646 T6537 T6536 arg2Of priming,without
R5647 T6537 T6538 arg1Of priming,(
R5648 T6540 T6538 arg2Of 6c,(
R5649 T6540 T6539 arg1Of 6c,Fig.
R5650 T6541 T6538 arg3Of ),(
R5651 T6546 T6545 arg1Of PBMCs,CF
R5652 T6546 T6547 arg1Of PBMCs,expressed
R5653 T6547 T6544 arg2Of expressed,if
R5654 T6551 T6547 arg2Of activity,expressed
R5655 T6551 T6548 arg2Of activity,increased
R5656 T6551 T6549 arg1Of activity,basal
R5657 T6551 T6550 arg1Of activity,NF-κB
R5658 T6553 T6554 arg1Of there,would
R5659 T6553 T6555 arg1Of there,be
R5660 T6555 T6542 arg1Of be,Similarly
R5661 T6555 T6543 arg1Of be,","
R5662 T6555 T6544 arg1Of be,if
R5663 T6555 T6552 arg1Of be,","
R5664 T6555 T6554 arg2Of be,would
R5665 T6557 T6555 arg2Of increase,be
R5666 T6557 T6556 arg1Of increase,an
R5667 T6557 T6558 arg1Of increase,in
R5668 T6557 T6561 arg1Of increase,in
R5669 T6560 T6558 arg2Of secretion,in
R5670 T6560 T6559 arg1Of secretion,IL-1β
R5671 T6563 T6561 arg2Of absence,in
R5672 T6563 T6562 arg1Of absence,the
R5673 T6563 T6564 arg1Of absence,of
R5674 T6566 T6564 arg2Of priming,of
R5675 T6566 T6565 arg1Of priming,LPS
R5676 T6570 T6569 arg1Of increase,no
R5677 T6570 T6571 arg1Of increase,in
R5678 T6570 T6573 arg1Of increase,was
R5679 T6570 T6574 arg2Of increase,observed
R5680 T6572 T6571 arg2Of IL-1β,in
R5681 T6574 T6567 arg1Of observed,However
R5682 T6574 T6568 arg1Of observed,","
R5683 T6574 T6573 arg2Of observed,was
R5684 T6574 T6575 arg1Of observed,under
R5685 T6579 T6575 arg2Of conditions,under
R5686 T6579 T6576 arg1Of conditions,basal
R5687 T6579 T6577 arg1Of conditions,or
R5688 T6579 T6578 arg2Of conditions,primed
R5689 T6580 T6581 arg1Of Studies,investigating
R5690 T6580 T6586 arg1Of Studies,have
R5691 T6580 T6587 arg1Of Studies,been
R5692 T6580 T6589 arg1Of Studies,inconsistent
R5693 T6583 T6581 arg2Of production,investigating
R5694 T6583 T6582 arg1Of production,the
R5695 T6583 T6584 arg1Of production,of
R5696 T6585 T6584 arg2Of IL-1β,of
R5697 T6587 T6586 arg2Of been,have
R5698 T6589 T6587 arg2Of inconsistent,been
R5699 T6589 T6588 arg1Of inconsistent,somewhat
R5700 T6591 T6590 arg1Of study,A
R5701 T6591 T6592 arg1Of study,by
R5702 T6591 T6595 arg1Of study,al.
R5703 T6591 T6596 arg1Of study,[
R5704 T6591 T6599 arg1Of study,provided
R5705 T6593 T6592 arg2Of Reininger,by
R5706 T6595 T6594 arg1Of al.,et
R5707 T6597 T6596 arg2Of 43,[
R5708 T6598 T6596 arg3Of ],[
R5709 T6600 T6599 arg2Of evidence,provided
R5710 T6605 T6602 arg1Of cells,human
R5711 T6605 T6603 arg1Of cells,bronchial
R5712 T6605 T6604 arg1Of cells,epithelial
R5713 T6605 T6606 arg1Of cells,possessing
R5714 T6605 T6611 arg1Of cells,had
R5715 T6605 T6620 arg1Of cells,lacked
R5716 T6610 T6606 arg2Of mutation,possessing
R5717 T6610 T6607 arg1Of mutation,the
R5718 T6610 T6608 arg1Of mutation,ΔF508
R5719 T6610 T6609 arg1Of mutation,CFTR
R5720 T6611 T6619 arg1Of had,and
R5721 T6614 T6613 arg1Of reduced,slightly
R5722 T6615 T6611 arg2Of capacity,had
R5723 T6615 T6612 arg1Of capacity,a
R5724 T6615 T6614 arg1Of capacity,reduced
R5725 T6615 T6616 modOf capacity,to
R5726 T6617 T6616 arg1Of produce,to
R5727 T6618 T6617 arg2Of IL-1β,produce
R5728 T6619 T6600 arg2Of and,evidence
R5729 T6619 T6601 arg1Of and,that
R5730 T6620 T6619 arg2Of lacked,and
R5731 T6622 T6620 arg2Of ability,lacked
R5732 T6622 T6621 arg1Of ability,the
R5733 T6622 T6623 modOf ability,to
R5734 T6624 T6623 arg1Of induce,to
R5735 T6624 T6629 arg1Of induce,in
R5736 T6628 T6624 arg2Of activation,induce
R5737 T6628 T6625 arg1Of activation,an
R5738 T6628 T6626 arg1Of activation,early
R5739 T6628 T6627 arg1Of activation,NF-κB
R5740 T6630 T6629 arg2Of response,in
R5741 T6630 T6631 arg1Of response,to
R5742 T6633 T6631 arg2Of aeruginosa,to
R5743 T6633 T6632 arg1Of aeruginosa,P.
R5744 T6637 T6636 arg1Of study,a
R5745 T6637 T6638 arg1Of study,by
R5746 T6637 T6641 arg1Of study,al.
R5747 T6637 T6642 arg1Of study,[
R5748 T6637 T6645 arg1Of study,found
R5749 T6639 T6638 arg2Of Kotrange,by
R5750 T6641 T6640 arg1Of al.,et
R5751 T6643 T6642 arg2Of 31,[
R5752 T6644 T6642 arg3Of ],[
R5753 T6645 T6634 arg1Of found,Conversely
R5754 T6645 T6635 arg1Of found,","
R5755 T6650 T6647 arg1Of macrophages,murine
R5756 T6650 T6648 arg1Of macrophages,bone
R5757 T6650 T6649 arg1Of macrophages,marrow-derived
R5758 T6650 T6651 arg1Of macrophages,expressing
R5759 T6650 T6653 arg1Of macrophages,produced
R5760 T6650 T6659 arg1Of macrophages,compared
R5761 T6652 T6651 arg2Of ΔF508-CFTR,expressing
R5762 T6653 T6645 arg2Of produced,found
R5763 T6653 T6646 arg1Of produced,that
R5764 T6653 T6658 arg1Of produced,when
R5765 T6655 T6653 arg2Of amounts,produced
R5766 T6655 T6654 arg2Of amounts,increased
R5767 T6655 T6656 arg1Of amounts,of
R5768 T6657 T6656 arg2Of IL-1β,of
R5769 T6659 T6658 arg2Of compared,when
R5770 T6659 T6660 arg1Of compared,to
R5771 T6661 T6660 arg2Of macrophages,to
R5772 T6661 T6662 arg1Of macrophages,expressing
R5773 T6662 T6665 arg1Of expressing,in
R5774 T6664 T6662 arg2Of CFTR,expressing
R5775 T6664 T6663 arg1Of CFTR,normal
R5776 T6666 T6665 arg2Of response,in
R5777 T6666 T6667 arg1Of response,to
R5778 T6670 T6667 arg2Of K56-2,to
R5779 T6670 T6668 arg1Of K56-2,Burkholderia
R5780 T6670 T6669 arg1Of K56-2,cenocepacia
R5781 T6672 T6671 arg1Of differentiation,The
R5782 T6672 T6673 arg1Of differentiation,of
R5783 T6672 T6675 arg1Of differentiation,into
R5784 T6672 T6677 arg1Of differentiation,may
R5785 T6672 T6679 arg1Of differentiation,account
R5786 T6674 T6673 arg2Of monocytes,of
R5787 T6676 T6675 arg2Of macrophages,into
R5788 T6679 T6677 arg2Of account,may
R5789 T6679 T6678 arg1Of account,partly
R5790 T6679 T6680 arg1Of account,for
R5791 T6679 T6694 arg1Of account,and
R5792 T6682 T6680 arg2Of differences,for
R5793 T6682 T6681 arg1Of differences,the
R5794 T6682 T6683 arg2Of differences,observed
R5795 T6683 T6684 arg1Of observed,with
R5796 T6686 T6684 arg2Of study,with
R5797 T6686 T6685 arg1Of study,the
R5798 T6686 T6687 arg1Of study,by
R5799 T6686 T6692 arg1Of study,by
R5800 T6691 T6687 arg2Of production,by
R5801 T6691 T6688 arg1Of production,Kotrange.
R5802 T6691 T6689 arg1Of production,Inflammasome-mediated
R5803 T6691 T6690 arg1Of production,IL-1β
R5804 T6693 T6692 arg2Of monocytes,by
R5805 T6694 T6708 arg1Of and,[
R5806 T6694 T6711 arg1Of and,and
R5807 T6695 T6696 arg1Of PBMCs,does
R5808 T6695 T6697 arg1Of PBMCs,differ
R5809 T6697 T6694 arg2Of differ,and
R5810 T6697 T6696 arg2Of differ,does
R5811 T6697 T6698 arg1Of differ,from
R5812 T6697 T6703 arg1Of differ,","
R5813 T6697 T6704 arg1Of differ,[
R5814 T6697 T6707 arg1Of differ,","
R5815 T6699 T6698 arg2Of macrophages,from
R5816 T6699 T6700 arg1Of macrophages,[
R5817 T6701 T6700 arg2Of 47,[
R5818 T6702 T6700 arg3Of ],[
R5819 T6705 T6704 arg2Of 48,[
R5820 T6706 T6704 arg3Of ],[
R5821 T6709 T6708 arg2Of 49,[
R5822 T6710 T6708 arg3Of ],[
R5823 T6712 T6713 arg1Of macrophages,are
R5824 T6712 T6714 arg2Of macrophages,found
R5825 T6712 T6716 arg1Of macrophages,have
R5826 T6714 T6711 arg2Of found,and
R5827 T6714 T6713 arg2Of found,are
R5828 T6716 T6714 arg3Of have,found
R5829 T6716 T6715 arg1Of have,to
R5830 T6718 T6716 arg2Of expression,have
R5831 T6718 T6717 arg1Of expression,higher
R5832 T6718 T6719 arg1Of expression,of
R5833 T6718 T6721 arg1Of expression,over
R5834 T6720 T6719 arg2Of CFTR,of
R5835 T6722 T6721 arg2Of monocytes,over
R5836 T6722 T6723 arg1Of monocytes,[
R5837 T6724 T6723 arg2Of 50,[
R5838 T6725 T6723 arg3Of ],[
R5839 T6729 T6730 arg1Of monocytes,and
R5840 T6730 T6733 arg1Of and,express
R5841 T6730 T6743 arg1Of and,produce
R5842 T6732 T6730 arg2Of PBMCs,and
R5843 T6732 T6731 arg1Of PBMCs,other
R5844 T6733 T6738 arg1Of express,","
R5845 T6733 T6739 arg1Of express,[
R5846 T6733 T6742 arg1Of express,and
R5847 T6734 T6733 arg2Of CFTR,express
R5848 T6734 T6735 arg1Of CFTR,[
R5849 T6736 T6735 arg2Of 50,[
R5850 T6737 T6735 arg3Of ],[
R5851 T6740 T6739 arg2Of 51,[
R5852 T6741 T6739 arg3Of ],[
R5853 T6742 T6728 arg2Of and,as
R5854 T6743 T6742 arg2Of produce,and
R5855 T6745 T6743 arg2Of amounts,produce
R5856 T6745 T6744 arg1Of amounts,large
R5857 T6745 T6746 arg1Of amounts,of
R5858 T6747 T6746 arg2Of IL-1β,of
R5859 T6749 T6750 arg1Of they,are
R5860 T6749 T6754 arg1Of they,examine
R5861 T6750 T6726 arg1Of are,However
R5862 T6750 T6727 arg1Of are,","
R5863 T6750 T6728 arg1Of are,as
R5864 T6750 T6748 arg1Of are,","
R5865 T6750 T6753 modOf are,to
R5866 T6752 T6750 arg2Of models,are
R5867 T6752 T6751 arg1Of models,adequate
R5868 T6754 T6753 arg1Of examine,to
R5869 T6756 T6754 arg2Of effects,examine
R5870 T6756 T6755 arg1Of effects,the
R5871 T6756 T6757 arg1Of effects,of
R5872 T6756 T6760 arg1Of effects,on
R5873 T6759 T6757 arg2Of function,of
R5874 T6759 T6758 arg1Of function,CFTR
R5875 T6762 T6760 arg2Of production,on
R5876 T6762 T6761 arg1Of production,IL-1β
R5877 T6765 T6763 arg1Of cells,Other
R5878 T6765 T6764 arg1Of cells,hematopoeitic
R5879 T6765 T6766 arg1Of cells,may
R5880 T6765 T6768 arg1Of cells,contribute
R5881 T6768 T6766 arg2Of contribute,may
R5882 T6768 T6767 arg1Of contribute,also
R5883 T6768 T6769 arg1Of contribute,to
R5884 T6771 T6769 arg2Of production,to
R5885 T6771 T6770 arg1Of production,IL-1β
R5886 T6773 T6772 arg2Of example,For
R5887 T6776 T6775 arg1Of counts,neutrophil
R5888 T6776 T6777 arg1Of counts,can
R5889 T6776 T6778 arg1Of counts,be
R5890 T6776 T6780 arg2Of counts,increased
R5891 T6776 T6799 arg1Of counts,may
R5892 T6776 T6800 arg1Of counts,produce
R5893 T6780 T6777 arg2Of increased,can
R5894 T6780 T6778 arg2Of increased,be
R5895 T6780 T6779 arg1Of increased,significantly
R5896 T6780 T6781 arg1Of increased,in
R5897 T6780 T6790 arg1Of increased,","
R5898 T6780 T6791 arg1Of increased,[
R5899 T6780 T6794 arg1Of increased,","
R5900 T6780 T6795 arg1Of increased,[
R5901 T6780 T6798 arg1Of increased,and
R5902 T6783 T6781 arg2Of lungs,in
R5903 T6783 T6782 arg1Of lungs,the
R5904 T6783 T6784 arg1Of lungs,of
R5905 T6786 T6784 arg2Of patients,of
R5906 T6786 T6785 arg1Of patients,CF
R5907 T6786 T6787 arg1Of patients,[
R5908 T6788 T6787 arg2Of 52,[
R5909 T6789 T6787 arg3Of ],[
R5910 T6792 T6791 arg2Of 53,[
R5911 T6793 T6791 arg3Of ],[
R5912 T6796 T6795 arg2Of 54,[
R5913 T6797 T6795 arg3Of ],[
R5914 T6798 T6772 arg1Of and,For
R5915 T6798 T6774 arg1Of and,","
R5916 T6800 T6798 arg2Of produce,and
R5917 T6800 T6799 arg2Of produce,may
R5918 T6800 T6803 arg1Of produce,through
R5919 T6802 T6800 arg2Of IL-1β,produce
R5920 T6802 T6801 arg1Of IL-1β,mature
R5921 T6806 T6803 arg2Of mechanisms,through
R5922 T6806 T6804 arg1Of mechanisms,caspase-1
R5923 T6806 T6805 arg1Of mechanisms,independent
R5924 T6806 T6807 arg1Of mechanisms,[
R5925 T6808 T6807 arg2Of 55,[
R5926 T6809 T6807 arg3Of ],[
R5927 T6811 T6810 arg1Of role,The
R5928 T6811 T6812 arg1Of role,of
R5929 T6811 T6815 arg1Of role,in
R5930 T6811 T6821 arg1Of role,is
R5931 T6811 T6823 arg1Of role,straightforward
R5932 T6814 T6812 arg2Of activation,of
R5933 T6814 T6813 arg1Of activation,NF-κB
R5934 T6817 T6816 arg1Of activation,inflammasome
R5935 T6817 T6818 arg1Of activation,and
R5936 T6818 T6815 arg2Of and,in
R5937 T6820 T6818 arg2Of secretion,and
R5938 T6820 T6819 arg1Of secretion,IL-1β
R5939 T6821 T6822 arg1Of is,not
R5940 T6823 T6821 arg2Of straightforward,is
R5941 T6824 T6825 arg1Of Studies,have
R5942 T6824 T6826 arg1Of Studies,revealed
R5943 T6826 T6825 arg2Of revealed,have
R5944 T6829 T6826 arg2Of role,revealed
R5945 T6829 T6827 arg1Of role,an
R5946 T6829 T6828 arg1Of role,essential
R5947 T6829 T6830 arg1Of role,for
R5948 T6832 T6830 arg2Of activation,for
R5949 T6832 T6831 arg1Of activation,NF-κB
R5950 T6832 T6833 arg1Of activation,in
R5951 T6835 T6833 arg2Of production,in
R5952 T6835 T6834 arg1Of production,the
R5953 T6835 T6836 arg1Of production,of
R5954 T6837 T6838 arg1Of pro-IL-1β,and
R5955 T6839 T6838 arg2Of inflammasome,and
R5956 T6840 T6836 arg2Of components,of
R5957 T6840 T6837 arg1Of components,pro-IL-1β
R5958 T6840 T6839 arg1Of components,inflammasome
R5959 T6840 T6842 arg1Of components,as
R5960 T6842 T6841 arg1Of as,such
R5961 T6843 T6842 arg2Of NLRP3,as
R5962 T6843 T6844 arg1Of NLRP3,[
R5963 T6843 T6847 arg1Of NLRP3,","
R5964 T6843 T6848 arg1Of NLRP3,[
R5965 T6845 T6844 arg2Of 22,[
R5966 T6846 T6844 arg3Of ],[
R5967 T6849 T6848 arg2Of 23,[
R5968 T6850 T6848 arg3Of ],[
R5969 T6852 T6851 arg2Of contrast,In
R5970 T6854 T6855 arg1Of deletion,of
R5971 T6854 T6865 arg1Of deletion,increases
R5972 T6854 T6879 arg1Of deletion,demonstrates
R5973 T6856 T6855 arg2Of IKKβ,of
R5974 T6856 T6857 arg1Of IKKβ,","
R5975 T6859 T6857 arg2Of kinase,","
R5976 T6859 T6858 arg1Of kinase,a
R5977 T6859 T6860 arg1Of kinase,essential
R5978 T6860 T6861 arg1Of essential,in
R5979 T6863 T6861 arg2Of activation,in
R5980 T6863 T6862 arg1Of activation,NF-κB
R5981 T6865 T6868 arg1Of increases,in
R5982 T6865 T6874 arg1Of increases,","
R5983 T6865 T6875 arg1Of increases,[
R5984 T6865 T6878 arg1Of increases,and
R5985 T6867 T6865 arg2Of secretion,increases
R5986 T6867 T6866 arg1Of secretion,IL-1β
R5987 T6870 T6868 arg2Of macrophages,in
R5988 T6870 T6869 arg1Of macrophages,murine
R5989 T6870 T6871 arg1Of macrophages,[
R5990 T6872 T6871 arg2Of 56,[
R5991 T6873 T6871 arg3Of ],[
R5992 T6876 T6875 arg2Of 57,[
R5993 T6877 T6875 arg3Of ],[
R5994 T6878 T6851 arg1Of and,In
R5995 T6878 T6853 arg1Of and,","
R5996 T6878 T6864 arg1Of and,","
R5997 T6879 T6878 arg2Of demonstrates,and
R5998 T6882 T6879 arg2Of role,demonstrates
R5999 T6882 T6880 arg1Of role,a
R6000 T6882 T6881 arg1Of role,dual
R6001 T6882 T6883 arg1Of role,for
R6002 T6882 T6885 arg1Of role,in
R6003 T6884 T6883 arg2Of NF-κB,for
R6004 T6886 T6885 arg2Of regulation,in
R6005 T6886 T6887 arg1Of regulation,of
R6006 T6888 T6887 arg2Of IL-1β,of
R6007 T6890 T6889 arg1Of address,To
R6008 T6892 T6890 arg2Of uncertainty,address
R6009 T6892 T6891 arg1Of uncertainty,this
R6010 T6892 T6893 arg1Of uncertainty,in
R6011 T6895 T6893 arg2Of experiments,in
R6012 T6895 T6894 arg1Of experiments,our
R6013 T6896 T6890 arg1Of we,address
R6014 T6896 T6898 arg1Of we,quantified
R6015 T6896 T6915 arg1Of we,found
R6016 T6898 T6914 arg1Of quantified,and
R6017 T6899 T6900 arg1Of IL-8,","
R6018 T6900 T6905 arg1Of ",",and
R6019 T6904 T6900 arg2Of cytokine,","
R6020 T6904 T6901 arg1Of cytokine,an
R6021 T6904 T6902 arg1Of cytokine,important
R6022 T6904 T6903 arg1Of cytokine,CF
R6023 T6905 T6898 arg2Of and,quantified
R6024 T6906 T6905 arg2Of marker,and
R6025 T6906 T6907 arg1Of marker,of
R6026 T6909 T6907 arg2Of activation,of
R6027 T6909 T6908 arg1Of activation,NF-κB
R6028 T6909 T6910 arg1Of activation,[
R6029 T6911 T6910 arg2Of 58,[
R6030 T6912 T6910 arg3Of ],[
R6031 T6914 T6889 modOf and,To
R6032 T6914 T6897 arg1Of and,also
R6033 T6914 T6913 arg1Of and,","
R6034 T6915 T6914 arg2Of found,and
R6035 T6917 T6915 arg2Of differences,found
R6036 T6917 T6916 arg1Of differences,no
R6037 T6917 T6918 arg1Of differences,between
R6038 T6919 T6920 arg1Of CF,and
R6039 T6920 T6918 arg2Of and,between
R6040 T6922 T6920 arg2Of subjects,and
R6041 T6922 T6921 arg1Of subjects,control
R6042 T6925 T6926 arg1Of levels,of
R6043 T6925 T6929 arg1Of levels,in
R6044 T6925 T6932 arg1Of levels,were
R6045 T6925 T6933 arg1Of levels,dependent
R6046 T6925 T6938 arg1Of levels,did
R6047 T6925 T6940 arg1Of levels,increase
R6048 T6928 T6926 arg2Of pro-IL-1β,of
R6049 T6928 T6927 arg1Of pro-IL-1β,intracellular
R6050 T6931 T6929 arg2Of cells,in
R6051 T6931 T6930 arg1Of cells,THP-1
R6052 T6932 T6937 arg1Of were,and
R6053 T6933 T6932 arg2Of dependent,were
R6054 T6933 T6934 arg1Of dependent,on
R6055 T6936 T6934 arg2Of activity,on
R6056 T6936 T6935 arg1Of activity,NF-κB
R6057 T6937 T6923 arg1Of and,Similarly
R6058 T6937 T6924 arg1Of and,","
R6059 T6940 T6937 arg2Of increase,and
R6060 T6940 T6938 arg2Of increase,did
R6061 T6940 T6939 arg1Of increase,not
R6062 T6940 T6941 arg1Of increase,with
R6063 T6943 T6941 arg2Of treatment,with
R6064 T6943 T6942 arg1Of treatment,CFTRinh172
R6065 T6945 T6944 arg1Of treatment,Subsequent
R6066 T6945 T6946 arg1Of treatment,of
R6067 T6945 T6951 arg1Of treatment,with
R6068 T6945 T6957 arg1Of treatment,inhibited
R6069 T6948 T6947 arg1Of cells,THP-1
R6070 T6948 T6949 arg1Of cells,and
R6071 T6949 T6946 arg2Of and,of
R6072 T6950 T6949 arg2Of PBMCs,and
R6073 T6955 T6951 arg2Of Bay11-7082,with
R6074 T6955 T6952 arg1Of Bay11-7082,the
R6075 T6955 T6953 arg1Of Bay11-7082,NF-κB
R6076 T6955 T6954 arg1Of Bay11-7082,inhibitor
R6077 T6957 T6956 arg1Of inhibited,significantly
R6078 T6957 T6963 arg1Of inhibited,(
R6079 T6959 T6960 arg1Of IL-1β,and
R6080 T6961 T6960 arg2Of IL-8,and
R6081 T6962 T6957 arg2Of secretion,inhibited
R6082 T6962 T6958 arg1Of secretion,both
R6083 T6962 T6959 arg1Of secretion,IL-1β
R6084 T6962 T6961 arg1Of secretion,IL-8
R6085 T6964 T6963 arg2Of Fig.,(
R6086 T6964 T6965 arg1Of Fig.,6d–h
R6087 T6966 T6963 arg3Of ),(
R6088 T6970 T6971 arg1Of IL-1β,and
R6089 T6972 T6971 arg2Of IL-8,and
R6090 T6973 T6969 arg1Of responses,the
R6091 T6973 T6970 arg1Of responses,IL-1β
R6092 T6973 T6972 arg1Of responses,IL-8
R6093 T6973 T6974 arg2Of responses,observed
R6094 T6973 T6975 arg1Of responses,were
R6095 T6973 T6977 arg1Of responses,dependent
R6096 T6975 T6967 arg1Of were,Therefore
R6097 T6975 T6968 arg1Of were,","
R6098 T6977 T6975 arg2Of dependent,were
R6099 T6977 T6976 arg1Of dependent,both
R6100 T6977 T6978 arg1Of dependent,upon
R6101 T6980 T6978 arg2Of activation,upon
R6102 T6980 T6979 arg1Of activation,NF-κB
R6103 T6981 T6982 arg1Of Priming,with
R6104 T6981 T6990 arg1Of Priming,is
R6105 T6981 T6991 arg1Of Priming,unable
R6106 T6981 T6993 arg1Of Priming,induce
R6107 T6981 T7010 arg1Of Priming,generated
R6108 T6985 T6982 arg2Of aeruginosa,with
R6109 T6985 T6983 arg1Of aeruginosa,heat-killed
R6110 T6985 T6984 arg1Of aeruginosa,P.
R6111 T6988 T6987 arg2Of LPS,like
R6112 T6990 T7009 arg1Of is,but
R6113 T6991 T6990 arg2Of unable,is
R6114 T6993 T6991 arg2Of induce,unable
R6115 T6993 T6992 arg1Of induce,to
R6116 T6993 T6998 arg1Of induce,as
R6117 T6997 T6993 arg2Of response,induce
R6118 T6997 T6994 arg1Of response,a
R6119 T6997 T6995 arg1Of response,strong
R6120 T6997 T6996 arg1Of response,IL-1β
R6121 T6999 T6998 arg2Of compared,as
R6122 T6999 T7000 modOf compared,to
R6123 T7001 T7000 arg1Of live,to
R6124 T7003 T7001 arg2Of aeruginosa,live
R6125 T7003 T7002 arg1Of aeruginosa,P.
R6126 T7003 T7004 arg1Of aeruginosa,(
R6127 T7006 T7004 arg2Of 6c,(
R6128 T7006 T7005 arg1Of 6c,Fig.
R6129 T7007 T7004 arg3Of ),(
R6130 T7009 T6986 arg1Of but,","
R6131 T7009 T6987 arg1Of but,like
R6132 T7009 T6989 arg1Of but,","
R6133 T7009 T7008 arg1Of but,","
R6134 T7010 T7009 arg2Of generated,but
R6135 T7010 T7028 arg1Of generated,despite
R6136 T7013 T7011 arg1Of activation,greater
R6137 T7013 T7012 arg1Of activation,NF-κB/AP-1
R6138 T7013 T7014 arg1Of activation,(
R6139 T7013 T7018 arg1Of activation,and
R6140 T7016 T7014 arg2Of 6a,(
R6141 T7016 T7015 arg1Of 6a,Fig.
R6142 T7017 T7014 arg3Of ),(
R6143 T7018 T7010 arg2Of and,generated
R6144 T7020 T7018 arg2Of secretion,and
R6145 T7020 T7019 arg1Of secretion,IL-8
R6146 T7020 T7021 arg1Of secretion,(
R6147 T7020 T7025 arg1Of secretion,than
R6148 T7023 T7021 arg2Of 6b,(
R6149 T7023 T7022 arg1Of 6b,Fig.
R6150 T7024 T7021 arg3Of ),(
R6151 T7027 T7025 arg2Of bacteria,than
R6152 T7027 T7026 arg1Of bacteria,live
R6153 T7029 T7028 arg2Of stimulation,despite
R6154 T7029 T7030 arg1Of stimulation,at
R6155 T7032 T7030 arg2Of MOIs,at
R6156 T7032 T7031 arg1Of MOIs,equivalent
R6157 T7033 T7034 arg1Of This,may
R6158 T7033 T7035 arg1Of This,be
R6159 T7033 T7036 arg1Of This,indicative
R6160 T7035 T7034 arg2Of be,may
R6161 T7035 T7050 arg1Of be,as
R6162 T7036 T7035 arg2Of indicative,be
R6163 T7036 T7037 arg1Of indicative,of
R6164 T7040 T7041 arg1Of degree,and
R6165 T7041 T7037 arg2Of and,of
R6166 T7041 T7038 arg1Of and,the
R6167 T7041 T7039 arg1Of and,different
R6168 T7041 T7043 arg1Of and,of
R6169 T7042 T7041 arg2Of quality,and
R6170 T7046 T7043 arg2Of response,of
R6171 T7046 T7044 arg1Of response,the
R6172 T7046 T7045 arg1Of response,inflammatory
R6173 T7046 T7047 arg2Of response,generated
R6174 T7047 T7048 arg1Of generated,by
R6175 T7049 T7048 arg2Of live,by
R6176 T7051 T7050 arg2Of opposed,as
R6177 T7051 T7052 arg1Of opposed,to
R6178 T7054 T7052 arg2Of bacteria,to
R6179 T7054 T7053 arg1Of bacteria,dead
R6180 T7054 T7055 arg1Of bacteria,[
R6181 T7056 T7055 arg2Of 59,[
R6182 T7057 T7055 arg3Of ],[
R6183 T7059 T7058 arg1Of shortcomings,Potential
R6184 T7059 T7060 arg1Of shortcomings,of
R6185 T7059 T7063 arg1Of shortcomings,include
R6186 T7062 T7060 arg2Of experiments,of
R6187 T7062 T7061 arg1Of experiments,these
R6188 T7065 T7063 arg2Of translatability,include
R6189 T7065 T7064 arg1Of translatability,its
R6190 T7065 T7066 arg1Of translatability,to
R6191 T7068 T7067 arg1Of disease,lung
R6192 T7068 T7069 arg1Of disease,and
R6193 T7069 T7066 arg2Of and,to
R6194 T7070 T7069 arg2Of issues,and
R6195 T7070 T7071 arg2Of issues,related
R6196 T7071 T7072 arg1Of related,to
R6197 T7071 T7078 arg1Of related,during
R6198 T7074 T7072 arg2Of hypermutability,to
R6199 T7074 T7073 arg1Of hypermutability,the
R6200 T7074 T7075 arg1Of hypermutability,of
R6201 T7077 T7075 arg2Of aeruginosa,of
R6202 T7077 T7076 arg1Of aeruginosa,P.
R6203 T7080 T7078 arg2Of evolution,during
R6204 T7080 T7079 arg1Of evolution,the
R6205 T7080 T7081 arg1Of evolution,of
R6206 T7083 T7081 arg2Of infection,of
R6207 T7083 T7082 arg1Of infection,chronic
R6208 T7086 T7085 arg1Of responses,the
R6209 T7086 T7087 arg2Of responses,measured
R6210 T7086 T7092 arg1Of responses,may
R6211 T7086 T7095 arg1Of responses,reflect
R6212 T7087 T7088 arg1Of measured,in
R6213 T7091 T7088 arg2Of cells,in
R6214 T7091 T7089 arg1Of cells,peripheral
R6215 T7091 T7090 arg1Of cells,blood
R6216 T7095 T7084 arg2Of reflect,Although
R6217 T7095 T7092 arg2Of reflect,may
R6218 T7095 T7093 arg1Of reflect,not
R6219 T7095 T7094 arg1Of reflect,completely
R6220 T7097 T7095 arg2Of responses,reflect
R6221 T7097 T7096 arg1Of responses,the
R6222 T7097 T7098 arg1Of responses,occurring
R6223 T7098 T7099 arg1Of occurring,in
R6224 T7102 T7099 arg2Of lung,in
R6225 T7102 T7100 arg1Of lung,the
R6226 T7102 T7101 arg1Of lung,CF
R6227 T7104 T7105 arg1Of PBMCs,have
R6228 T7105 T7111 arg1Of have,:
R6229 T7107 T7105 arg2Of number,have
R6230 T7107 T7106 arg1Of number,a
R6231 T7107 T7108 arg1Of number,of
R6232 T7110 T7108 arg2Of advantages,of
R6233 T7110 T7109 arg1Of advantages,useful
R6234 T7111 T7133 arg1Of :,and
R6235 T7113 T7112 arg1Of i,(
R6236 T7113 T7114 arg1Of i,)
R6237 T7115 T7116 arg1Of PBMCs,are
R6238 T7115 T7118 arg1Of PBMCs,subject
R6239 T7116 T7111 arg2Of are,:
R6240 T7116 T7113 arg1Of are,i
R6241 T7116 T7117 arg1Of are,not
R6242 T7118 T7116 arg2Of subject,are
R6243 T7118 T7119 arg1Of subject,to
R6244 T7120 T7119 arg2Of alterations,to
R6245 T7120 T7121 arg1Of alterations,that
R6246 T7120 T7122 arg1Of alterations,may
R6247 T7120 T7123 arg1Of alterations,emerge
R6248 T7123 T7122 arg2Of emerge,may
R6249 T7123 T7124 arg1Of emerge,from
R6250 T7127 T7125 arg1Of culture,long-term
R6251 T7127 T7126 arg1Of culture,cell
R6252 T7127 T7128 arg1Of culture,","
R6253 T7128 T7130 arg1Of ",",and
R6254 T7129 T7128 arg2Of cloning,","
R6255 T7130 T7124 arg2Of and,from
R6256 T7131 T7130 arg2Of immortalization,and
R6257 T7133 T7084 arg1Of and,Although
R6258 T7133 T7103 arg1Of and,","
R6259 T7133 T7132 arg1Of and,","
R6260 T7135 T7134 arg1Of ii,(
R6261 T7135 T7136 arg1Of ii,)
R6262 T7137 T7138 arg1Of PBMCs,express
R6263 T7137 T7147 arg1Of PBMCs,secrete
R6264 T7138 T7146 arg1Of express,and
R6265 T7141 T7138 arg2Of repertoire,express
R6266 T7141 T7139 arg1Of repertoire,a
R6267 T7141 T7140 arg1Of repertoire,large
R6268 T7141 T7142 arg1Of repertoire,of
R6269 T7145 T7142 arg2Of receptors,of
R6270 T7145 T7143 arg1Of receptors,innate
R6271 T7145 T7144 arg1Of receptors,immune
R6272 T7146 T7133 arg2Of and,and
R6273 T7146 T7135 arg1Of and,ii
R6274 T7147 T7146 arg2Of secrete,and
R6275 T7150 T7147 arg2Of array,secrete
R6276 T7150 T7148 arg1Of array,a
R6277 T7150 T7149 arg1Of array,broad
R6278 T7150 T7151 arg1Of array,of
R6279 T7152 T7153 arg1Of cytokines,and
R6280 T7153 T7151 arg2Of and,of
R6281 T7153 T7155 arg1Of and,allowing
R6282 T7154 T7153 arg2Of chemokines,and
R6283 T7157 T7155 arg2Of analysis,allowing
R6284 T7157 T7156 arg1Of analysis,comprehensive
R6285 T7157 T7158 arg1Of analysis,of
R6286 T7160 T7158 arg2Of modulation,of
R6287 T7160 T7159 arg1Of modulation,the
R6288 T7160 T7161 arg1Of modulation,of
R6289 T7160 T7164 arg1Of modulation,by
R6290 T7163 T7161 arg2Of responses,of
R6291 T7163 T7162 arg1Of responses,inflammatory
R6292 T7165 T7164 arg2Of CFTR,by
R6293 T7166 T7167 arg1Of Consideration,must
R6294 T7166 T7169 arg1Of Consideration,be
R6295 T7166 T7170 arg3Of Consideration,given
R6296 T7170 T7167 arg2Of given,must
R6297 T7170 T7168 arg1Of given,also
R6298 T7170 T7169 arg2Of given,be
R6299 T7173 T7170 arg2Of nature,given
R6300 T7173 T7171 arg2Of nature,to
R6301 T7173 T7172 arg1Of nature,the
R6302 T7173 T7174 arg1Of nature,of
R6303 T7177 T7175 arg1Of infection,P.
R6304 T7177 T7176 arg1Of infection,aeruginosa
R6305 T7177 T7178 arg1Of infection,and
R6306 T7178 T7174 arg2Of and,of
R6307 T7180 T7178 arg2Of changes,and
R6308 T7180 T7179 arg1Of changes,genotypic
R6309 T7180 T7181 arg1Of changes,in
R6310 T7183 T7181 arg2Of aeruginosa,in
R6311 T7183 T7182 arg1Of aeruginosa,P.
R6312 T7183 T7184 arg1Of aeruginosa,as
R6313 T7186 T7184 arg2Of progresses,as
R6314 T7186 T7185 arg1Of progresses,infection
R6315 T7188 T7187 arg1Of aeruginosa,P.
R6316 T7188 T7189 arg1Of aeruginosa,mediates
R6317 T7189 T7192 arg1Of mediates,through
R6318 T7189 T7208 arg1Of mediates,","
R6319 T7189 T7209 arg1Of mediates,[
R6320 T7191 T7189 arg2Of activation,mediates
R6321 T7191 T7190 arg1Of activation,inflammasome
R6322 T7197 T7193 arg1Of system,its
R6323 T7197 T7194 arg1Of system,type
R6324 T7197 T7195 arg1Of system,III
R6325 T7197 T7196 arg1Of system,secretion
R6326 T7197 T7198 arg1Of system,(
R6327 T7197 T7201 arg1Of system,and
R6328 T7199 T7198 arg2Of T3SS,(
R6329 T7200 T7198 arg3Of ),(
R6330 T7201 T7192 arg2Of and,through
R6331 T7204 T7201 arg2Of inflammasome,and
R6332 T7204 T7202 arg1Of inflammasome,the
R6333 T7204 T7203 arg1Of inflammasome,NLRC4
R6334 T7204 T7205 arg1Of inflammasome,[
R6335 T7206 T7205 arg2Of 19,[
R6336 T7207 T7205 arg3Of ],[
R6337 T7210 T7209 arg2Of 20,[
R6338 T7211 T7209 arg3Of ],[
R6339 T7214 T7215 arg1Of clones,of
R6340 T7214 T7222 arg1Of clones,may
R6341 T7214 T7223 arg1Of clones,accumulate
R6342 T7217 T7215 arg2Of aeruginosa,of
R6343 T7217 T7216 arg1Of aeruginosa,P.
R6344 T7217 T7218 arg2Of aeruginosa,established
R6345 T7218 T7219 arg1Of established,during
R6346 T7221 T7219 arg2Of infection,during
R6347 T7221 T7220 arg1Of infection,chronic
R6348 T7223 T7212 arg1Of accumulate,However
R6349 T7223 T7213 arg1Of accumulate,","
R6350 T7223 T7222 arg2Of accumulate,may
R6351 T7223 T7225 arg1Of accumulate,in
R6352 T7224 T7223 arg2Of mutations,accumulate
R6353 T7227 T7225 arg2Of factors,in
R6354 T7227 T7226 arg1Of factors,virulence
R6355 T7227 T7229 arg1Of factors,as
R6356 T7229 T7228 arg1Of as,such
R6357 T7230 T7229 arg2Of exsA,as
R6358 T7230 T7231 arg1Of exsA,[
R6359 T7232 T7231 arg2Of 60,[
R6360 T7233 T7231 arg3Of ],[
R6361 T7235 T7234 arg2Of employing,By
R6362 T7238 T7235 arg2Of mutant,employing
R6363 T7238 T7236 arg1Of mutant,a
R6364 T7238 T7237 arg1Of mutant,deletion
R6365 T7238 T7239 arg1Of mutant,in
R6366 T7240 T7239 arg2Of exsA,in
R6367 T7240 T7241 arg1Of exsA,","
R6368 T7244 T7241 arg2Of regulator,","
R6369 T7244 T7242 arg1Of regulator,the
R6370 T7244 T7243 arg1Of regulator,key
R6371 T7244 T7245 arg1Of regulator,in
R6372 T7247 T7245 arg2Of transcription,in
R6373 T7247 T7246 arg1Of transcription,T3SS
R6374 T7249 T7235 arg1Of we,employing
R6375 T7249 T7250 arg1Of we,confirmed
R6376 T7250 T7234 arg1Of confirmed,By
R6377 T7250 T7248 arg1Of confirmed,","
R6378 T7253 T7252 arg1Of T3SS,the
R6379 T7253 T7254 arg1Of T3SS,is
R6380 T7253 T7255 arg1Of T3SS,important
R6381 T7254 T7251 arg1Of is,that
R6382 T7254 T7264 arg1Of is,and
R6383 T7255 T7254 arg2Of important,is
R6384 T7255 T7256 arg1Of important,for
R6385 T7258 T7256 arg2Of secretion,for
R6386 T7258 T7257 arg1Of secretion,IL-1β
R6387 T7258 T7259 arg1Of secretion,(
R6388 T7261 T7259 arg2Of 5a–b,(
R6389 T7261 T7260 arg1Of 5a–b,Fig.
R6390 T7262 T7259 arg3Of ),(
R6391 T7264 T7250 arg2Of and,confirmed
R6392 T7264 T7263 arg1Of and,","
R6393 T7265 T7264 arg2Of that,and
R6394 T7267 T7266 arg2Of on,depending
R6395 T7269 T7267 arg2Of adaptation,on
R6396 T7269 T7268 arg1Of adaptation,the
R6397 T7269 T7270 arg1Of adaptation,in
R6398 T7273 T7270 arg2Of secretion,in
R6399 T7273 T7271 arg1Of secretion,type
R6400 T7273 T7272 arg1Of secretion,III
R6401 T7278 T7275 arg1Of response,the
R6402 T7278 T7276 arg1Of response,host
R6403 T7278 T7277 arg1Of response,IL-1β
R6404 T7278 T7279 arg1Of response,may
R6405 T7278 T7280 arg1Of response,be
R6406 T7278 T7281 arg1Of response,up
R6407 T7278 T7283 arg2Of response,downregulated
R6408 T7280 T7282 arg1Of be,or
R6409 T7281 T7280 arg2Of up,be
R6410 T7282 T7265 arg2Of or,that
R6411 T7282 T7266 arg1Of or,depending
R6412 T7282 T7274 arg1Of or,","
R6413 T7282 T7279 arg2Of or,may
R6414 T7282 T7287 arg1Of or,","
R6415 T7282 T7288 arg1Of or,[
R6416 T7282 T7291 arg1Of or,","
R6417 T7282 T7292 arg1Of or,[
R6418 T7283 T7282 arg2Of downregulated,or
R6419 T7283 T7284 arg1Of downregulated,[
R6420 T7285 T7284 arg2Of 19,[
R6421 T7286 T7284 arg3Of ],[
R6422 T7289 T7288 arg2Of 60,[
R6423 T7290 T7288 arg3Of ],[
R6424 T7293 T7292 arg2Of 61,[
R6425 T7294 T7292 arg3Of ],[
R6426 T7296 T7295 arg2Of conclusion,In
R6427 T7299 T7298 arg1Of data,our
R6428 T7299 T7300 arg1Of data,are
R6429 T7299 T7301 arg1Of data,consistent
R6430 T7299 T7310 arg1Of data,airway
R6431 T7300 T7308 arg1Of are,","
R6432 T7301 T7300 arg2Of consistent,are
R6433 T7301 T7302 arg1Of consistent,with
R6434 T7304 T7302 arg2Of role,with
R6435 T7304 T7303 arg1Of role,a
R6436 T7304 T7305 arg1Of role,for
R6437 T7307 T7305 arg2Of cells,for
R6438 T7307 T7306 arg1Of cells,hematopoietic
R6439 T7308 T7295 arg1Of ",",In
R6440 T7308 T7297 arg1Of ",",","
R6441 T7310 T7308 arg2Of airway,","
R6442 T7310 T7309 arg1Of airway,not
R6443 T7310 T7313 arg1Of airway,","
R6444 T7310 T7314 arg1Of airway,as
R6445 T7310 T7325 arg1Of airway,in
R6446 T7312 T7310 arg2Of cells,airway
R6447 T7312 T7311 arg1Of cells,epithelial
R6448 T7317 T7314 arg2Of source,as
R6449 T7317 T7315 arg1Of source,the
R6450 T7317 T7316 arg1Of source,major
R6451 T7317 T7318 arg1Of source,of
R6452 T7321 T7318 arg2Of production,of
R6453 T7321 T7319 arg1Of production,inflammasome-mediated
R6454 T7321 T7320 arg1Of production,IL-1β
R6455 T7321 T7322 arg1Of production,in
R6456 T7324 T7322 arg2Of lungs,in
R6457 T7324 T7323 arg1Of lungs,the
R6458 T7326 T7325 arg2Of response,in
R6459 T7326 T7327 arg1Of response,to
R6460 T7328 T7329 arg1Of ATP,and
R6461 T7329 T7327 arg2Of and,to
R6462 T7331 T7329 arg2Of aeruginosa,and
R6463 T7331 T7330 arg1Of aeruginosa,P.
R6464 T7334 T7335 arg1Of we,find
R6465 T7335 T7332 arg1Of find,Furthermore
R6466 T7335 T7333 arg1Of find,","
R6467 T7337 T7335 arg2Of evidence,find
R6468 T7337 T7336 arg1Of evidence,little
R6469 T7337 T7339 arg1Of evidence,support
R6470 T7339 T7335 arg3Of support,find
R6471 T7339 T7338 arg1Of support,to
R6472 T7344 T7339 arg2Of response,support
R6473 T7344 T7340 arg1Of response,an
R6474 T7344 T7341 arg2Of response,increased
R6475 T7344 T7342 arg1Of response,IL-1β
R6476 T7344 T7343 arg1Of response,inflammatory
R6477 T7344 T7345 arg1Of response,to
R6478 T7347 T7345 arg2Of stimulation,to
R6479 T7347 T7346 arg1Of stimulation,NF-κB/Inflammasome
R6480 T7347 T7348 arg1Of stimulation,in
R6481 T7350 T7348 arg2Of patients,in
R6482 T7350 T7349 arg1Of patients,CF
R6483 T7352 T7351 arg1Of studies,Further
R6484 T7352 T7353 arg1Of studies,are
R6485 T7352 T7354 arg2Of studies,warranted
R6486 T7352 T7356 arg1Of studies,determine
R6487 T7354 T7353 arg2Of warranted,are
R6488 T7356 T7354 arg3Of determine,warranted
R6489 T7356 T7355 arg1Of determine,to
R6490 T7358 T7359 arg1Of adaptations,of
R6491 T7358 T7369 arg1Of adaptations,alters
R6492 T7361 T7359 arg2Of aeruginosa,of
R6493 T7361 T7360 arg1Of aeruginosa,P.
R6494 T7361 T7362 arg1Of aeruginosa,during
R6495 T7364 T7362 arg2Of course,during
R6496 T7364 T7363 arg1Of course,the
R6497 T7364 T7365 arg1Of course,of
R6498 T7368 T7365 arg2Of infection,of
R6499 T7368 T7366 arg1Of infection,chronic
R6500 T7368 T7367 arg1Of infection,lung
R6501 T7369 T7357 arg1Of alters,if
R6502 T7369 T7373 arg1Of alters,and
R6503 T7371 T7369 arg2Of activation,alters
R6504 T7371 T7370 arg1Of activation,inflammasome
R6505 T7373 T7356 arg2Of and,determine
R6506 T7373 T7372 arg1Of and,","
R6507 T7374 T7373 arg2Of whether,and
R6508 T7375 T7376 arg1Of this,can
R6509 T7375 T7377 arg1Of this,be
R6510 T7375 T7378 arg2Of this,correlated
R6511 T7378 T7374 arg2Of correlated,whether
R6512 T7378 T7376 arg2Of correlated,can
R6513 T7378 T7377 arg2Of correlated,be
R6514 T7378 T7379 arg1Of correlated,with
R6515 T7381 T7379 arg2Of severity,with
R6516 T7381 T7380 arg1Of severity,disease
R6517 T7381 T7382 arg1Of severity,in
R6518 T7383 T7382 arg2Of CF,in
R8128 T9314 T9313 arg1Of Statement,Ethics
R8129 T9316 T9315 arg1Of samples,Blood
R8130 T9316 T9317 arg1Of samples,were
R8131 T9316 T9318 arg2Of samples,obtained
R8132 T9318 T9317 arg2Of obtained,were
R8133 T9318 T9319 arg1Of obtained,with
R8134 T9318 T9326 arg1Of obtained,and
R8135 T9322 T9319 arg2Of consent,with
R8136 T9322 T9320 arg2Of consent,informed
R8137 T9322 T9321 arg2Of consent,written
R8138 T9322 T9323 arg1Of consent,from
R8139 T9325 T9323 arg2Of subjects,from
R8140 T9325 T9324 arg1Of subjects,control
R8141 T9328 T9327 arg1Of patients,CF
R8142 T9328 T9329 arg1Of patients,at
R8143 T9328 T9336 arg1Of patients,was
R8144 T9328 T9337 arg2Of patients,obtained
R8145 T9332 T9330 arg1Of Children,the
R8146 T9332 T9331 arg1Of Children,BC
R8147 T9332 T9333 arg2Of Children,'s
R8148 T9335 T9329 arg2Of Consent,at
R8149 T9335 T9333 arg1Of Consent,'s
R8150 T9335 T9334 arg1Of Consent,Hospital.
R8151 T9337 T9326 arg2Of obtained,and
R8152 T9337 T9336 arg2Of obtained,was
R8153 T9337 T9338 arg1Of obtained,for
R8154 T9339 T9338 arg2Of children,for
R8155 T9339 T9340 arg1Of children,by
R8156 T9342 T9343 arg1Of parent,or
R8157 T9343 T9340 arg2Of or,by
R8158 T9343 T9341 arg1Of or,their
R8159 T9345 T9343 arg2Of guardian,or
R8160 T9345 T9344 arg1Of guardian,legal
R8161 T9346 T9348 arg1Of Subjects,years
R8162 T9346 T9352 arg1Of Subjects,older
R8163 T9346 T9353 arg1Of Subjects,were
R8164 T9346 T9354 arg2Of Subjects,required
R8165 T9348 T9347 arg1Of years,7
R8166 T9348 T9349 arg1Of years,of
R8167 T9348 T9351 arg1Of years,and
R8168 T9350 T9349 arg2Of age,of
R8169 T9352 T9351 arg2Of older,and
R8170 T9354 T9353 arg2Of required,were
R8171 T9354 T9355 modOf required,to
R8172 T9356 T9355 arg1Of provide,to
R8173 T9356 T9360 arg1Of provide,well
R8174 T9358 T9356 arg2Of assent,provide
R8175 T9358 T9357 arg1Of assent,informed
R8176 T9360 T9359 arg1Of well,as
R8177 T9361 T9362 arg1Of Protocols,were
R8178 T9361 T9363 arg2Of Protocols,approved
R8179 T9363 T9362 arg2Of approved,were
R8180 T9369 T9363 arg1Of Board,approved
R8181 T9369 T9364 arg2Of Board,by
R8182 T9369 T9365 arg1Of Board,the
R8183 T9369 T9366 arg1Of Board,Clinical
R8184 T9369 T9367 arg1Of Board,Research
R8185 T9369 T9368 arg1Of Board,Ethics
R8186 T9369 T9370 arg1Of Board,(
R8187 T9371 T9370 arg2Of H09-01192,(
R8188 T9372 T9370 arg3Of ),(
R8253 T9470 T9469 arg1Of Culture,Cell
R8254 T9471 T9472 arg1Of CF,(
R8255 T9471 T9477 arg1Of CF,and
R8256 T9473 T9474 arg1Of IB3-1,and
R8257 T9474 T9472 arg2Of and,(
R8258 T9475 T9474 arg2Of CuFi-1,and
R8259 T9476 T9472 arg3Of ),(
R8260 T9477 T9495 arg1Of and,were
R8261 T9477 T9496 arg2Of and,derived
R8262 T9478 T9477 arg2Of control,and
R8263 T9478 T9479 arg1Of control,(
R8264 T9480 T9481 arg1Of S9,and
R8265 T9481 T9479 arg2Of and,(
R8266 T9482 T9481 arg2Of NuLi-1,and
R8267 T9483 T9479 arg3Of ),(
R8268 T9484 T9485 arg1Of cells,were
R8269 T9484 T9486 arg2Of cells,obtained
R8270 T9486 T9485 arg2Of obtained,were
R8271 T9486 T9487 arg1Of obtained,from
R8272 T9492 T9489 arg1Of Collection.,American
R8273 T9492 T9490 arg1Of Collection.,Type
R8274 T9492 T9491 arg1Of Collection.,Culture
R8275 T9494 T9487 arg2Of cells,from
R8276 T9494 T9488 arg1Of cells,the
R8277 T9494 T9492 arg1Of cells,Collection.
R8278 T9494 T9493 arg1Of cells,IB3-1
R8279 T9496 T9485 modOf derived,were
R8280 T9496 T9495 arg2Of derived,were
R8281 T9496 T9497 arg1Of derived,from
R8282 T9496 T9506 arg1Of derived,and
R8283 T9499 T9497 arg2Of patient,from
R8284 T9499 T9498 arg1Of patient,a
R8285 T9499 T9500 arg1Of patient,expressing
R8286 T9502 T9503 arg1Of ΔF508,and
R8287 T9504 T9503 arg2Of W1282X,and
R8288 T9505 T9500 arg2Of mutations,expressing
R8289 T9505 T9501 arg1Of mutations,the
R8290 T9505 T9502 arg1Of mutations,ΔF508
R8291 T9505 T9504 arg1Of mutations,W1282X
R8292 T9507 T9508 arg1Of CuFi-1,were
R8293 T9507 T9509 arg2Of CuFi-1,derived
R8294 T9509 T9506 arg2Of derived,and
R8295 T9509 T9508 arg2Of derived,were
R8296 T9509 T9510 arg1Of derived,from
R8297 T9514 T9510 arg2Of patient,from
R8298 T9514 T9511 arg1Of patient,a
R8299 T9514 T9512 arg1Of patient,ΔF508
R8300 T9514 T9513 arg1Of patient,homozygous
R8301 T9516 T9515 arg1Of cells,S9
R8302 T9516 T9517 arg1Of cells,are
R8303 T9517 T9531 arg1Of are,and
R8304 T9519 T9517 arg2Of cells,are
R8305 T9519 T9518 arg1Of cells,IB3-1
R8306 T9519 T9520 arg1Of cells,that
R8307 T9519 T9521 arg1Of cells,have
R8308 T9519 T9522 arg1Of cells,been
R8309 T9519 T9523 arg2Of cells,transfected
R8310 T9523 T9521 arg2Of transfected,have
R8311 T9523 T9522 arg2Of transfected,been
R8312 T9523 T9524 arg1Of transfected,with
R8313 T9525 T9524 arg2Of CFTR,with
R8314 T9525 T9526 arg1Of CFTR,using
R8315 T9530 T9526 arg2Of vector,using
R8316 T9530 T9527 arg1Of vector,an
R8317 T9530 T9528 arg1Of vector,adeno-associated
R8318 T9530 T9529 arg1Of vector,viral
R8319 T9533 T9532 arg1Of cells,NuLi-1
R8320 T9533 T9534 arg1Of cells,were
R8321 T9533 T9535 arg2Of cells,derived
R8322 T9535 T9531 arg2Of derived,and
R8323 T9535 T9534 arg2Of derived,were
R8324 T9535 T9536 arg1Of derived,from
R8325 T9538 T9536 arg2Of patient,from
R8326 T9538 T9537 arg1Of patient,a
R8327 T9538 T9539 arg1Of patient,possessing
R8328 T9543 T9539 arg2Of genotype,possessing
R8329 T9543 T9540 arg1Of genotype,a
R8330 T9543 T9541 arg1Of genotype,wild-type
R8331 T9543 T9542 arg1Of genotype,CFTR
R8332 T9545 T9544 arg1Of cells,THP1-XBlue
R8333 T9545 T9547 arg1Of cells,express
R8334 T9547 T9546 arg1Of express,stably
R8335 T9554 T9553 arg2Of SEAP,(
R8336 T9555 T9553 arg3Of ),(
R8337 T9556 T9547 arg2Of reporter,express
R8338 T9556 T9548 arg1Of reporter,a
R8339 T9556 T9549 arg2Of reporter,secreted
R8340 T9556 T9550 arg1Of reporter,embryonic
R8341 T9556 T9551 arg1Of reporter,alkaline
R8342 T9556 T9552 arg1Of reporter,phosphatase
R8343 T9556 T9553 arg1Of reporter,(
R8344 T9556 T9557 arg1Of reporter,inducible
R8345 T9557 T9558 arg1Of inducible,by
R8346 T9559 T9560 arg1Of NF-κB,and
R8347 T9560 T9558 arg2Of and,by
R8348 T9560 T9562 arg1Of and,(
R8349 T9561 T9560 arg2Of AP-1,and
R8350 T9563 T9562 arg2Of Invivogen,(
R8351 T9564 T9562 arg3Of ),(
R8352 T9565 T9566 arg1Of Cells,were
R8353 T9565 T9567 arg2Of Cells,cultured
R8354 T9567 T9566 arg2Of cultured,were
R8355 T9567 T9568 arg1Of cultured,as
R8356 T9567 T9574 modOf cultured,using
R8357 T9569 T9568 arg2Of recommended,as
R8358 T9573 T9569 arg1Of suppliers,recommended
R8359 T9573 T9570 arg2Of suppliers,by
R8360 T9573 T9571 arg1Of suppliers,their
R8361 T9573 T9572 arg1Of suppliers,respective
R8362 T9576 T9574 arg2Of protocols,using
R8363 T9576 T9575 arg1Of protocols,standard
R8364 T9577 T9578 arg1Of S9,and
R8365 T9579 T9578 arg2Of IB3-1,and
R8366 T9580 T9577 arg1Of cells,S9
R8367 T9580 T9579 arg1Of cells,IB3-1
R8368 T9580 T9581 arg1Of cells,were
R8369 T9580 T9582 arg2Of cells,cultured
R8370 T9582 T9581 arg2Of cultured,were
R8371 T9582 T9583 arg1Of cultured,in
R8372 T9585 T9584 arg1Of LHC-8,basal
R8373 T9585 T9586 arg1Of LHC-8,(
R8374 T9585 T9589 arg2Of LHC-8,supplemented
R8375 T9587 T9586 arg2Of Invitrogen,(
R8376 T9588 T9586 arg3Of ),(
R8377 T9589 T9590 arg1Of supplemented,with
R8378 T9592 T9590 arg2Of %,with
R8379 T9592 T9591 arg1Of %,10
R8380 T9594 T9593 arg2Of v/v,(
R8381 T9595 T9593 arg3Of ),(
R8382 T9598 T9585 arg1Of serum,LHC-8
R8383 T9598 T9593 arg1Of serum,(
R8384 T9598 T9596 arg1Of serum,fetal
R8385 T9598 T9597 arg1Of serum,bovine
R8386 T9598 T9599 arg1Of serum,(
R8387 T9598 T9602 arg1Of serum,","
R8388 T9600 T9599 arg2Of FBS,(
R8389 T9601 T9599 arg3Of ),(
R8390 T9602 T9607 arg1Of ",",and
R8391 T9603 T9604 arg1Of 2,mM
R8392 T9605 T9602 arg2Of L-glutamine,","
R8393 T9605 T9603 arg1Of L-glutamine,2
R8394 T9607 T9583 arg2Of and,in
R8395 T9607 T9606 arg1Of and,","
R8396 T9608 T9609 arg1Of 1,mM
R8397 T9611 T9607 arg2Of pyruvate,and
R8398 T9611 T9608 arg1Of pyruvate,1
R8399 T9611 T9610 arg1Of pyruvate,sodium
R8400 T9613 T9612 arg1Of cells,NuLi-1/CuFi-1
R8401 T9613 T9614 arg1Of cells,were
R8402 T9613 T9615 arg2Of cells,cultured
R8403 T9615 T9614 arg2Of cultured,were
R8404 T9615 T9616 arg1Of cultured,in
R8405 T9619 T9617 arg1Of medium,BEBM
R8406 T9619 T9618 arg1Of medium,serum-free
R8407 T9619 T9620 arg1Of medium,(
R8408 T9619 T9623 arg1Of medium,with
R8409 T9619 T9653 arg1Of medium,and
R8410 T9621 T9620 arg2Of Lonza,(
R8411 T9622 T9620 arg3Of ),(
R8412 T9626 T9624 arg1Of kit,supplement
R8413 T9626 T9625 arg1Of kit,bullet
R8414 T9626 T9627 arg1Of kit,(
R8415 T9626 T9648 arg1Of kit,","
R8416 T9628 T9629 arg1Of EGF,","
R8417 T9628 T9631 arg1Of EGF,","
R8418 T9628 T9635 arg1Of EGF,","
R8419 T9628 T9637 arg1Of EGF,","
R8420 T9630 T9629 arg2Of hydrocortisone,","
R8421 T9634 T9631 arg2Of extract,","
R8422 T9634 T9632 arg1Of extract,bovine
R8423 T9634 T9633 arg1Of extract,pituitary
R8424 T9636 T9635 arg2Of transferrin,","
R8425 T9637 T9627 arg2Of ",",(
R8426 T9637 T9640 arg1Of ",",","
R8427 T9639 T9637 arg2Of insulin,","
R8428 T9639 T9638 arg1Of insulin,bovine
R8429 T9641 T9640 arg2Of triiodothyronine,","
R8430 T9641 T9642 arg1Of triiodothyronine,","
R8431 T9641 T9644 arg1Of triiodothyronine,","
R8432 T9643 T9642 arg2Of epinephrine,","
R8433 T9646 T9644 arg2Of acid,","
R8434 T9646 T9645 arg1Of acid,retinoic
R8435 T9647 T9627 arg3Of ),(
R8436 T9648 T9623 arg2Of ",",with
R8437 T9649 T9650 arg1Of 2,mM
R8438 T9651 T9648 arg2Of L-glutamine,","
R8439 T9651 T9649 arg1Of L-glutamine,2
R8440 T9653 T9616 arg2Of and,in
R8441 T9653 T9652 arg1Of and,","
R8442 T9654 T9655 arg1Of 1,mM
R8443 T9657 T9653 arg2Of pyruvate,and
R8444 T9657 T9654 arg1Of pyruvate,1
R8445 T9657 T9656 arg1Of pyruvate,sodium
R8446 T9658 T9659 arg1Of PBMCs,from
R8447 T9658 T9664 arg1Of PBMCs,were
R8448 T9658 T9665 arg2Of PBMCs,cultured
R8449 T9661 T9660 arg1Of patients,CF
R8450 T9661 T9662 arg1Of patients,and
R8451 T9662 T9659 arg2Of and,from
R8452 T9663 T9662 arg2Of controls,and
R8453 T9665 T9664 arg2Of cultured,were
R8454 T9665 T9666 arg1Of cultured,in
R8455 T9667 T9668 arg1Of RPMI-1640,(
R8456 T9667 T9671 arg2Of RPMI-1640,supplemented
R8457 T9667 T9681 arg1Of RPMI-1640,and
R8458 T9669 T9668 arg2Of Hyclone,(
R8459 T9670 T9668 arg3Of ),(
R8460 T9671 T9672 arg1Of supplemented,with
R8461 T9674 T9673 arg1Of %,10
R8462 T9675 T9672 arg2Of FBS,with
R8463 T9675 T9674 arg1Of FBS,%
R8464 T9675 T9676 arg1Of FBS,","
R8465 T9677 T9678 arg1Of 2,mM
R8466 T9679 T9676 arg2Of L-glutamine,","
R8467 T9679 T9677 arg1Of L-glutamine,2
R8468 T9681 T9666 arg2Of and,in
R8469 T9681 T9680 arg1Of and,","
R8470 T9682 T9683 arg1Of 1,mM
R8471 T9685 T9681 arg2Of pyruvate,and
R8472 T9685 T9682 arg1Of pyruvate,1
R8473 T9685 T9684 arg1Of pyruvate,sodium
R8474 T9685 T9686 arg1Of pyruvate,(
R8475 T9688 T9686 arg2Of RPMI,(
R8476 T9688 T9687 arg1Of RPMI,complete
R8477 T9689 T9686 arg3Of ),(
R8478 T9691 T9690 arg1Of cells,THP1-XBlue
R8479 T9691 T9692 arg1Of cells,were
R8480 T9691 T9693 arg2Of cells,cultured
R8481 T9693 T9692 arg2Of cultured,were
R8482 T9693 T9694 arg1Of cultured,in
R8483 T9693 T9697 arg1Of cultured,with
R8484 T9696 T9694 arg2Of RPMI,in
R8485 T9696 T9695 arg1Of RPMI,complete
R8486 T9699 T9697 arg2Of addition,with
R8487 T9699 T9698 arg1Of addition,the
R8488 T9699 T9700 arg1Of addition,of
R8489 T9699 T9706 modOf addition,to
R8490 T9701 T9700 arg2Of zeocin,of
R8491 T9701 T9702 arg1Of zeocin,(
R8492 T9704 T9702 arg2Of µg/ml,(
R8493 T9704 T9703 arg1Of µg/ml,100
R8494 T9705 T9702 arg3Of ),(
R8495 T9707 T9706 arg1Of select,to
R8496 T9707 T9708 arg1Of select,for
R8497 T9709 T9708 arg2Of cells,for
R8498 T9709 T9710 arg1Of cells,expressing
R8499 T9714 T9710 arg2Of reporter,expressing
R8500 T9714 T9711 arg1Of reporter,the
R8501 T9714 T9712 arg1Of reporter,SEAP
R8502 T9714 T9713 arg1Of reporter,NF-κB/AP-1
R8503 T9715 T9716 arg1Of Prior,to
R8504 T9717 T9716 arg2Of stimulation,to
R8505 T9722 T9719 arg1Of lines,bronchial
R8506 T9722 T9720 arg1Of lines,epithelial
R8507 T9722 T9721 arg1Of lines,cell
R8508 T9722 T9723 arg1Of lines,were
R8509 T9722 T9724 arg2Of lines,plated
R8510 T9724 T9715 arg1Of plated,Prior
R8511 T9724 T9718 arg1Of plated,","
R8512 T9724 T9723 arg2Of plated,were
R8513 T9724 T9725 arg1Of plated,in
R8514 T9724 T9736 arg1Of plated,at
R8515 T9724 T9739 arg1Of plated,unless
R8516 T9728 T9727 arg2Of 62,[
R8517 T9729 T9727 arg3Of ],[
R8518 T9731 T9725 arg2Of plates,in
R8519 T9731 T9726 arg1Of plates,coated
R8520 T9731 T9727 arg1Of plates,[
R8521 T9731 T9730 arg1Of plates,96-well
R8522 T9731 T9732 arg1Of plates,(
R8523 T9733 T9732 arg2Of BD,(
R8524 T9733 T9734 arg1Of BD,Biosciences
R8525 T9735 T9732 arg3Of ),(
R8526 T9738 T9736 arg2Of cells/well,at
R8527 T9738 T9737 arg1Of cells/well,3×104
R8528 T9740 T9742 arg1Of indicated,and
R8529 T9742 T9739 arg2Of and,unless
R8530 T9742 T9741 arg1Of and,","
R8531 T9743 T9742 arg2Of allowed,and
R8532 T9745 T9743 arg3Of adhere,allowed
R8533 T9745 T9744 arg1Of adhere,to
R8534 T9745 T9746 arg1Of adhere,overnight
R8535 T9747 T9748 arg1Of Plates,for
R8536 T9747 T9753 arg1Of Plates,were
R8537 T9747 T9754 arg2Of Plates,coated
R8538 T9749 T9750 arg1Of S9,and
R8539 T9751 T9750 arg2Of IB3-1,and
R8540 T9752 T9748 arg2Of stimulations,for
R8541 T9752 T9749 arg1Of stimulations,S9
R8542 T9752 T9751 arg1Of stimulations,IB3-1
R8543 T9754 T9753 arg2Of coated,were
R8544 T9754 T9755 arg1Of coated,in
R8545 T9757 T9755 arg2Of mixture,in
R8546 T9757 T9756 arg1Of mixture,a
R8547 T9757 T9758 arg1Of mixture,of
R8548 T9761 T9759 arg1Of albumin,bovine
R8549 T9761 T9760 arg1Of albumin,serum
R8550 T9761 T9762 arg1Of albumin,(
R8551 T9761 T9766 arg1Of albumin,","
R8552 T9764 T9762 arg2Of µg/ml,(
R8553 T9764 T9763 arg1Of µg/ml,100
R8554 T9765 T9762 arg3Of ),(
R8555 T9766 T9773 arg1Of ",",and
R8556 T9767 T9766 arg2Of fibronectin,","
R8557 T9767 T9768 arg1Of fibronectin,(
R8558 T9770 T9768 arg2Of µg/ml,(
R8559 T9770 T9769 arg1Of µg/ml,10
R8560 T9771 T9768 arg3Of ),(
R8561 T9773 T9758 arg2Of and,of
R8562 T9773 T9772 arg1Of and,","
R8563 T9773 T9782 arg1Of and,(
R8564 T9776 T9773 arg2Of type,and
R8565 T9776 T9774 arg1Of type,bovine
R8566 T9776 T9775 arg1Of type,collage
R8567 T9776 T9777 arg1Of type,I
R8568 T9776 T9778 arg1Of type,(
R8569 T9780 T9778 arg2Of µg/ml,(
R8570 T9780 T9779 arg1Of µg/ml,30
R8571 T9781 T9778 arg3Of ),(
R8572 T9784 T9782 arg2Of Biosciences,(
R8573 T9784 T9783 arg1Of Biosciences,BD
R8574 T9785 T9782 arg3Of ),(
R8575 T9786 T9787 arg1Of Plates,for
R8576 T9786 T9791 arg1Of Plates,were
R8577 T9786 T9792 arg2Of Plates,coated
R8578 T9788 T9789 arg1Of NuLi-1,and
R8579 T9789 T9787 arg2Of and,for
R8580 T9790 T9789 arg2Of CuFi-1,and
R8581 T9792 T9791 arg2Of coated,were
R8582 T9792 T9793 arg1Of coated,with
R8583 T9795 T9793 arg2Of type,with
R8584 T9795 T9794 arg1Of type,collagen
R8585 T9795 T9796 arg1Of type,IV
R8586 T9795 T9797 arg1Of type,(
R8587 T9795 T9801 arg1Of type,(
R8588 T9799 T9797 arg2Of µg/ml,(
R8589 T9799 T9798 arg1Of µg/ml,60
R8590 T9800 T9797 arg3Of ),(
R8591 T9803 T9801 arg2Of Aldrich,(
R8592 T9803 T9802 arg1Of Aldrich,Sigma
R8593 T9804 T9801 arg3Of ),(
R8594 T9805 T9806 arg1Of PBMCs,were
R8595 T9805 T9807 arg2Of PBMCs,plated
R8596 T9807 T9806 arg2Of plated,were
R8597 T9807 T9808 arg1Of plated,in
R8598 T9807 T9811 arg1Of plated,at
R8599 T9810 T9808 arg2Of plates,in
R8600 T9810 T9809 arg1Of plates,96-well
R8601 T9813 T9811 arg2Of density,at
R8602 T9813 T9812 arg1Of density,a
R8603 T9813 T9814 arg1Of density,of
R8604 T9813 T9817 arg1Of density,in
R8605 T9816 T9814 arg2Of cells/well,of
R8606 T9816 T9815 arg1Of cells/well,1.5×105
R8607 T9819 T9817 arg2Of µl,in
R8608 T9819 T9818 arg1Of µl,200
R8609 T9819 T9820 arg1Of µl,(
R8610 T9822 T9820 arg2Of cells/mL,(
R8611 T9822 T9821 arg1Of cells/mL,7.5×105
R8612 T9823 T9820 arg3Of ),(
R8613 T9826 T9824 arg1Of cells,THP-1
R8614 T9826 T9825 arg1Of cells,reporter
R8615 T9826 T9827 arg1Of cells,were
R8616 T9826 T9828 arg2Of cells,differentiated
R8617 T9828 T9827 arg2Of differentiated,were
R8618 T9828 T9829 arg1Of differentiated,into
R8619 T9832 T9829 arg2Of phenotype,into
R8620 T9832 T9830 arg1Of phenotype,a
R8621 T9832 T9831 arg1Of phenotype,macrophage-like
R8622 T9832 T9833 arg1Of phenotype,using
R8623 T9833 T9847 arg1Of using,for
R8624 T9835 T9833 arg2Of ng/ml,using
R8625 T9835 T9834 arg1Of ng/ml,50
R8626 T9835 T9836 arg1Of ng/ml,of
R8627 T9839 T9836 arg2Of 13-acetate,of
R8628 T9839 T9837 arg1Of 13-acetate,phorbol
R8629 T9839 T9838 arg1Of 13-acetate,12-myristate
R8630 T9839 T9840 arg1Of 13-acetate,(
R8631 T9839 T9843 arg1Of 13-acetate,(
R8632 T9841 T9840 arg2Of PMA,(
R8633 T9842 T9840 arg3Of ),(
R8634 T9845 T9843 arg2Of Aldrich,(
R8635 T9845 T9844 arg1Of Aldrich,Sigma
R8636 T9846 T9843 arg3Of ),(
R8637 T9849 T9847 arg2Of hours,for
R8638 T9849 T9848 arg1Of hours,24
R8639 T9849 T9850 arg1Of hours,at
R8640 T9852 T9850 arg2Of density,at
R8641 T9852 T9851 arg1Of density,a
R8642 T9852 T9853 arg1Of density,of
R8643 T9852 T9856 arg1Of density,in
R8644 T9855 T9853 arg2Of cells/well,of
R8645 T9855 T9854 arg1Of cells/well,1×105
R8646 T9858 T9856 arg2Of µl,in
R8647 T9858 T9857 arg1Of µl,200
R8648 T9858 T9859 arg1Of µl,(
R8649 T9861 T9859 arg2Of cells/ml,(
R8650 T9861 T9860 arg1Of cells/ml,5×105
R8651 T9862 T9859 arg3Of ),(
R8652 T9863 T9864 arg1Of Cells,were
R8653 T9863 T9865 arg2Of Cells,washed
R8654 T9863 T9869 arg2Of Cells,allowed
R8655 T9863 T9871 arg1Of Cells,rest
R8656 T9865 T9866 arg1Of washed,with
R8657 T9865 T9868 arg1Of washed,and
R8658 T9867 T9866 arg2Of PBS,with
R8659 T9868 T9864 arg2Of and,were
R8660 T9869 T9868 arg2Of allowed,and
R8661 T9871 T9869 arg3Of rest,allowed
R8662 T9871 T9870 arg1Of rest,to
R8663 T9871 T9876 arg1Of rest,prior
R8664 T9875 T9871 arg2Of hours,rest
R8665 T9875 T9872 arg1Of hours,a
R8666 T9875 T9873 arg1Of hours,further
R8667 T9875 T9874 arg1Of hours,42
R8668 T9876 T9877 arg1Of prior,to
R8669 T9878 T9877 arg2Of stimulation,to
R9132 T10424 T10425 arg1Of CF,and
R9133 T10426 T10425 arg2Of control,and
R9134 T10428 T10424 arg1Of PBMCs,CF
R9135 T10428 T10426 arg1Of PBMCs,control
R9136 T10428 T10427 arg1Of PBMCs,subject
R9137 T10430 T10429 arg1Of diagnosis,The
R9138 T10430 T10431 arg1Of diagnosis,of
R9139 T10430 T10433 arg1Of diagnosis,was
R9140 T10430 T10434 arg2Of diagnosis,established
R9141 T10432 T10431 arg2Of CF,of
R9142 T10434 T10433 arg2Of established,was
R9143 T10438 T10436 arg1Of features,typical
R9144 T10438 T10437 arg1Of features,clinical
R9145 T10438 T10439 arg1Of features,","
R9146 T10439 T10450 arg1Of ",",and
R9147 T10443 T10439 arg2Of concentrations,","
R9148 T10443 T10440 arg2Of concentrations,increased
R9149 T10443 T10441 arg1Of concentrations,sweat
R9150 T10443 T10442 arg1Of concentrations,chloride
R9151 T10443 T10444 arg1Of concentrations,(
R9152 T10446 T10445 arg1Of 60,>
R9153 T10447 T10444 arg2Of mmol/l,(
R9154 T10447 T10446 arg1Of mmol/l,60
R9155 T10448 T10444 arg3Of ),(
R9156 T10450 T10434 arg1Of and,established
R9157 T10450 T10435 arg2Of and,by
R9158 T10450 T10449 arg1Of and,","
R9159 T10451 T10450 arg2Of detection,and
R9160 T10451 T10452 arg1Of detection,of
R9161 T10454 T10452 arg2Of mutations,of
R9162 T10454 T10453 arg1Of mutations,CF-inducing
R9163 T10456 T10455 arg1Of patients,All
R9164 T10456 T10457 arg1Of patients,with
R9165 T10456 T10459 arg1Of patients,were
R9166 T10456 T10461 arg1Of patients,stable
R9167 T10458 T10457 arg2Of CF,with
R9168 T10459 T10469 arg1Of were,and
R9169 T10461 T10459 arg2Of stable,were
R9170 T10461 T10460 arg1Of stable,clinically
R9171 T10461 T10462 arg1Of stable,at
R9172 T10464 T10462 arg2Of time,at
R9173 T10464 T10463 arg1Of time,the
R9174 T10464 T10465 arg1Of time,of
R9175 T10467 T10465 arg2Of donation,of
R9176 T10467 T10466 arg1Of donation,blood
R9177 T10469 T10468 arg1Of and,","
R9178 T10470 T10471 arg1Of we,excluded
R9179 T10471 T10469 arg2Of excluded,and
R9180 T10473 T10471 arg2Of subjects,excluded
R9181 T10473 T10472 arg1Of subjects,any
R9182 T10473 T10474 arg1Of subjects,who
R9183 T10473 T10475 arg1Of subjects,were
R9184 T10473 T10476 arg1Of subjects,receiving
R9185 T10476 T10475 arg2Of receiving,were
R9186 T10478 T10476 arg2Of corticosteroids,receiving
R9187 T10478 T10477 arg1Of corticosteroids,systemic
R9188 T10478 T10479 arg1Of corticosteroids,due
R9189 T10479 T10480 arg1Of due,to
R9190 T10483 T10480 arg2Of activity,to
R9191 T10483 T10481 arg1Of activity,potential
R9192 T10483 T10482 arg1Of activity,immunomodulatory
R9193 T10485 T10484 arg1Of samples,Control
R9194 T10485 T10486 arg1Of samples,were
R9195 T10485 T10487 arg2Of samples,provided
R9196 T10487 T10486 arg2Of provided,were
R9197 T10490 T10489 arg1Of adult,healthy
R9198 T10491 T10487 arg1Of volunteers,provided
R9199 T10491 T10488 arg2Of volunteers,by
R9200 T10491 T10490 arg1Of volunteers,adult
R9201 T10494 T10493 arg1Of published,previously
R9202 T10495 T10492 arg2Of work,In
R9203 T10495 T10494 arg1Of work,published
R9204 T10496 T10497 arg1Of we,have
R9205 T10496 T10498 arg1Of we,demonstrated
R9206 T10498 T10492 arg1Of demonstrated,In
R9207 T10498 T10497 arg2Of demonstrated,have
R9208 T10502 T10500 arg1Of responses,TLR-mediated
R9209 T10502 T10501 arg1Of responses,inflammatory
R9210 T10502 T10503 arg1Of responses,are
R9211 T10502 T10504 arg1Of responses,stable
R9212 T10502 T10518 arg1Of responses,did
R9213 T10502 T10520 arg1Of responses,age-match
R9214 T10503 T10516 arg1Of are,","
R9215 T10504 T10503 arg2Of stable,are
R9216 T10504 T10505 arg1Of stable,in
R9217 T10506 T10505 arg2Of humans,in
R9218 T10506 T10507 arg1Of humans,from
R9219 T10508 T10507 arg2Of birth,from
R9220 T10508 T10512 arg1Of birth,old
R9221 T10508 T10513 arg1Of birth,[
R9222 T10511 T10510 arg1Of years,60
R9223 T10512 T10509 arg1Of old,to
R9224 T10512 T10511 arg1Of old,years
R9225 T10514 T10513 arg2Of 63,[
R9226 T10515 T10513 arg3Of ],[
R9227 T10516 T10498 arg2Of ",",demonstrated
R9228 T10516 T10499 arg1Of ",",that
R9229 T10520 T10516 arg2Of age-match,","
R9230 T10520 T10517 arg1Of age-match,therefore
R9231 T10520 T10518 arg2Of age-match,did
R9232 T10520 T10519 arg1Of age-match,not
R9233 T10523 T10522 arg1Of patients,CF
R9234 T10523 T10524 arg1Of patients,and
R9235 T10524 T10520 arg2Of and,age-match
R9236 T10524 T10521 arg1Of and,the
R9237 T10526 T10524 arg2Of subjects,and
R9238 T10526 T10525 arg1Of subjects,control
R9239 T10528 T10527 arg1Of blood,Peripheral
R9240 T10528 T10529 arg1Of blood,was
R9241 T10528 T10530 arg2Of blood,collected
R9242 T10530 T10529 arg2Of collected,was
R9243 T10530 T10531 arg1Of collected,in
R9244 T10530 T10539 arg1Of collected,and
R9245 T10534 T10531 arg2Of tubes,in
R9246 T10534 T10532 arg1Of tubes,sodium
R9247 T10534 T10533 arg1Of tubes,heparin
R9248 T10534 T10535 arg1Of tubes,(
R9249 T10537 T10535 arg2Of Biosciences,(
R9250 T10537 T10536 arg1Of Biosciences,BD
R9251 T10538 T10535 arg3Of ),(
R9252 T10540 T10541 arg1Of PBMCs,were
R9253 T10540 T10542 arg2Of PBMCs,isolated
R9254 T10542 T10539 arg2Of isolated,and
R9255 T10542 T10541 arg2Of isolated,were
R9256 T10542 T10543 modOf isolated,using
R9257 T10546 T10543 arg2Of centrifugation,using
R9258 T10546 T10544 arg1Of centrifugation,density
R9259 T10546 T10545 arg1Of centrifugation,gradient
R9260 T10546 T10547 arg1Of centrifugation,on
R9261 T10549 T10547 arg2Of Plus,on
R9262 T10549 T10548 arg1Of Plus,Ficoll-Paque™
R9263 T10549 T10550 arg1Of Plus,(
R9264 T10552 T10550 arg2Of Healthcare,(
R9265 T10552 T10551 arg1Of Healthcare,GE
R9266 T10553 T10550 arg3Of ),(
R9267 T10557 T10554 arg1Of PBMCs,The
R9268 T10557 T10555 arg1Of PBMCs,layer
R9269 T10557 T10556 arg1Of PBMCs,containing
R9270 T10557 T10558 arg1Of PBMCs,was
R9271 T10557 T10559 arg2Of PBMCs,isolated
R9272 T10557 T10561 arg2Of PBMCs,washed
R9273 T10557 T10566 arg1Of PBMCs,resuspended
R9274 T10559 T10560 arg1Of isolated,","
R9275 T10560 T10565 arg1Of ",",and
R9276 T10561 T10560 arg2Of washed,","
R9277 T10561 T10562 arg1Of washed,twice
R9278 T10561 T10563 arg1Of washed,in
R9279 T10564 T10563 arg2Of PBS,in
R9280 T10565 T10558 arg2Of and,was
R9281 T10566 T10565 arg2Of resuspended,and
R9282 T10566 T10567 arg1Of resuspended,in
R9283 T10569 T10567 arg2Of RPMI,in
R9284 T10569 T10568 arg1Of RPMI,complete
R9285 T10570 T10571 arg1Of Cells,were
R9286 T10570 T10572 arg2Of Cells,enumerated
R9287 T10572 T10571 arg2Of enumerated,were
R9288 T10576 T10572 arg1Of exclusion,enumerated
R9289 T10576 T10573 arg2Of exclusion,by
R9290 T10576 T10574 arg1Of exclusion,trypan
R9291 T10576 T10575 arg1Of exclusion,blue
R9292 T10576 T10577 arg1Of exclusion,using
R9293 T10582 T10577 arg2Of counter,using
R9294 T10582 T10578 arg1Of counter,the
R9295 T10582 T10579 arg1Of counter,Countess
R9296 T10582 T10580 arg2Of counter,automated
R9297 T10582 T10581 arg1Of counter,cell
R9298 T10582 T10583 arg1Of counter,(
R9299 T10584 T10583 arg2Of Invitrogen,(
R9300 T10585 T10583 arg3Of ),(
R9301 T10587 T10586 arg2Of derivation,For
R9302 T10587 T10588 arg1Of derivation,of
R9303 T10587 T10590 arg1Of derivation,from
R9304 T10589 T10588 arg2Of macrophages,of
R9305 T10591 T10590 arg2Of monocytes,from
R9306 T10593 T10594 arg1Of monocytes,were
R9307 T10593 T10595 arg2Of monocytes,allowed
R9308 T10593 T10597 arg1Of monocytes,adhere
R9309 T10595 T10586 arg1Of allowed,For
R9310 T10595 T10592 arg1Of allowed,","
R9311 T10595 T10594 arg2Of allowed,were
R9312 T10597 T10595 arg3Of adhere,allowed
R9313 T10597 T10596 arg1Of adhere,to
R9314 T10597 T10598 arg1Of adhere,to
R9315 T10597 T10600 arg1Of adhere,for
R9316 T10599 T10598 arg2Of plastic,to
R9317 T10602 T10600 arg2Of hours,for
R9318 T10602 T10601 arg1Of hours,2
R9319 T10602 T10603 arg1Of hours,in
R9320 T10602 T10606 arg2Of hours,after
R9321 T10602 T10607 arg1Of hours,which
R9322 T10604 T10603 arg2Of RPMI,in
R9323 T10604 T10605 arg1Of RPMI,1640
R9324 T10609 T10608 arg1Of cells,non-adherent
R9325 T10609 T10610 arg1Of cells,were
R9326 T10609 T10611 arg2Of cells,removed
R9327 T10611 T10606 arg1Of removed,after
R9328 T10611 T10610 arg2Of removed,were
R9329 T10612 T10613 arg1Of Monocytes,were
R9330 T10612 T10614 arg2Of Monocytes,allowed
R9331 T10612 T10616 arg1Of Monocytes,differentiate
R9332 T10614 T10613 arg2Of allowed,were
R9333 T10616 T10614 arg3Of differentiate,allowed
R9334 T10616 T10615 arg1Of differentiate,to
R9335 T10616 T10617 arg1Of differentiate,in
R9336 T10616 T10627 arg1Of differentiate,for
R9337 T10618 T10617 arg2Of RPMI,in
R9338 T10618 T10619 arg1Of RPMI,1640
R9339 T10618 T10620 arg2Of RPMI,supplemented
R9340 T10620 T10621 arg1Of supplemented,with
R9341 T10622 T10623 arg1Of 10,%
R9342 T10626 T10621 arg2Of serum,with
R9343 T10626 T10622 arg1Of serum,10
R9344 T10626 T10624 arg1Of serum,human
R9345 T10626 T10625 arg1Of serum,AB
R9346 T10629 T10627 arg2Of days,for
R9347 T10629 T10628 arg1Of days,10
R9564 T10873 T10872 arg1Of stimulation,Cell
R9565 T10873 T10874 arg1Of stimulation,and
R9566 T10876 T10874 arg2Of quantification,and
R9567 T10876 T10875 arg1Of quantification,cytokine
R9568 T10879 T10877 arg1Of cells,Bronchial
R9569 T10879 T10878 arg1Of cells,epithelial
R9570 T10879 T10880 arg1Of cells,were
R9571 T10879 T10881 arg2Of cells,plated
R9572 T10879 T10883 arg2Of cells,allowed
R9573 T10879 T10885 arg1Of cells,adhere
R9574 T10881 T10882 arg1Of plated,and
R9575 T10882 T10880 arg2Of and,were
R9576 T10883 T10882 arg2Of allowed,and
R9577 T10885 T10883 arg3Of adhere,allowed
R9578 T10885 T10884 arg1Of adhere,to
R9579 T10886 T10888 arg1Of overnight,to
R9580 T10888 T10887 arg1Of to,prior
R9581 T10889 T10885 arg2Of stimulation,adhere
R9582 T10889 T10886 arg1Of stimulation,overnight
R9583 T10892 T10890 arg1Of cells,Bronchial
R9584 T10892 T10891 arg1Of cells,epithelial
R9585 T10892 T10893 arg1Of cells,were
R9586 T10892 T10894 arg2Of cells,rested
R9587 T10892 T10896 arg2Of cells,primed
R9588 T10892 T10903 arg2Of cells,stimulated
R9589 T10894 T10895 arg1Of rested,or
R9590 T10895 T10893 arg2Of or,were
R9591 T10895 T10914 arg2Of or,indicated
R9592 T10896 T10897 arg1Of primed,with
R9593 T10896 T10899 arg1Of primed,for
R9594 T10896 T10902 arg1Of primed,and
R9595 T10898 T10897 arg2Of LPS,with
R9596 T10901 T10899 arg2Of hours,for
R9597 T10901 T10900 arg1Of hours,5
R9598 T10902 T10895 arg2Of and,or
R9599 T10903 T10902 arg2Of stimulated,and
R9600 T10903 T10904 arg1Of stimulated,with
R9601 T10906 T10904 arg2Of P.,with
R9602 T10906 T10905 arg1Of P.,live
R9603 T10908 T10907 arg1Of PAO1,aeruginosa
R9604 T10908 T10909 arg1Of PAO1,or
R9605 T10909 T10911 arg1Of or,for
R9606 T10909 T10914 arg1Of or,indicated
R9607 T10910 T10909 arg2Of ATP,or
R9608 T10913 T10911 arg2Of times,for
R9609 T10913 T10912 arg1Of times,the
R9610 T10915 T10916 arg1Of PBMCs,and
R9611 T10916 T10920 arg1Of and,were
R9612 T10916 T10922 arg2Of and,rested
R9613 T10916 T10924 arg2Of and,primed
R9614 T10919 T10916 arg2Of cells,and
R9615 T10919 T10917 arg1Of cells,THP-1
R9616 T10919 T10918 arg1Of cells,reporter
R9617 T10922 T10923 arg1Of rested,or
R9618 T10923 T10920 arg2Of or,were
R9619 T10923 T10921 arg1Of or,either
R9620 T10924 T10923 arg2Of primed,or
R9621 T10924 T10925 arg1Of primed,with
R9622 T10926 T10927 arg1Of LPS,(
R9623 T10926 T10930 arg1Of LPS,or
R9624 T10928 T10927 arg2Of Invivogen,(
R9625 T10929 T10927 arg3Of ),(
R9626 T10930 T10925 arg2Of or,with
R9627 T10933 T10930 arg2Of overnight,or
R9628 T10933 T10931 arg1Of overnight,heat-killed
R9629 T10933 T10932 arg1Of overnight,PAO1
R9630 T10933 T10934 arg1Of overnight,(
R9631 T10936 T10934 arg2Of hours,(
R9632 T10936 T10935 arg1Of hours,16
R9633 T10937 T10934 arg3Of ),(
R9634 T10940 T10939 arg1Of day,next
R9635 T10940 T10956 arg1Of day,or
R9636 T10942 T10941 arg1Of cells,the
R9637 T10942 T10943 arg1Of cells,were
R9638 T10942 T10944 arg2Of cells,challenged
R9639 T10944 T10940 arg2Of challenged,day
R9640 T10944 T10943 arg2Of challenged,were
R9641 T10944 T10945 arg1Of challenged,with
R9642 T10947 T10945 arg2Of PAO1,with
R9643 T10947 T10946 arg1Of PAO1,live
R9644 T10947 T10948 arg1Of PAO1,","
R9645 T10947 T10950 arg1Of PAO1,","
R9646 T10949 T10948 arg2Of PAO1ΔexsA,","
R9647 T10951 T10950 arg2Of ATP,","
R9648 T10951 T10952 arg1Of ATP,(
R9649 T10953 T10952 arg2Of Invivogen,(
R9650 T10954 T10952 arg3Of ),(
R9651 T10956 T10938 arg1Of or,The
R9652 T10956 T10955 arg1Of or,","
R9653 T10956 T10970 arg1Of or,indicated
R9654 T10957 T10956 arg2Of Poly,or
R9655 T10957 T10958 arg1Of Poly,(
R9656 T10957 T10963 arg1Of Poly,(
R9657 T10957 T10967 arg1Of Poly,for
R9658 T10959 T10958 arg2Of dA,(
R9659 T10959 T10960 arg1Of dA,:
R9660 T10959 T10961 arg1Of dA,dT
R9661 T10962 T10958 arg3Of ),(
R9662 T10965 T10963 arg2Of Aldrich,(
R9663 T10965 T10964 arg1Of Aldrich,Sigma
R9664 T10966 T10963 arg3Of ),(
R9665 T10969 T10967 arg2Of times,for
R9666 T10969 T10968 arg1Of times,the
R9667 T10970 T10971 arg1Of indicated,(
R9668 T10972 T10971 arg2Of see,(
R9669 T10973 T10972 arg2Of Fig.,see
R9670 T10973 T10974 arg1Of Fig.,1
R9671 T10975 T10971 arg3Of ),(
R9672 T10977 T10976 arg2Of stimulations,For
R9673 T10977 T10978 arg1Of stimulations,with
R9674 T10979 T10978 arg2Of Poly,with
R9675 T10979 T10980 arg1Of Poly,(
R9676 T10981 T10980 arg2Of dA,(
R9677 T10981 T10982 arg1Of dA,:
R9678 T10981 T10983 arg1Of dA,dT
R9679 T10984 T10980 arg3Of ),(
R9680 T10987 T10986 arg1Of LTX,lipofectamine
R9681 T10987 T10988 arg1Of LTX,was
R9682 T10987 T10989 arg2Of LTX,used
R9683 T10987 T11006 arg1Of LTX,was
R9684 T10987 T11007 arg2Of LTX,mixed
R9685 T10989 T10988 arg2Of used,was
R9686 T10989 T10990 arg1Of used,at
R9687 T10989 T11005 arg1Of used,and
R9688 T10994 T10993 arg2Of w∶v,(
R9689 T10995 T10993 arg3Of ),(
R9690 T10996 T10990 arg2Of ratio,at
R9691 T10996 T10991 arg1Of ratio,a
R9692 T10996 T10992 arg1Of ratio,1∶1
R9693 T10996 T10993 arg1Of ratio,(
R9694 T10996 T10997 arg1Of ratio,of
R9695 T10996 T11001 modOf ratio,to
R9696 T10998 T10997 arg2Of µg,of
R9697 T10998 T10999 arg1Of µg,of
R9698 T11000 T10999 arg2Of DNA,of
R9699 T11002 T11001 arg1Of µl,to
R9700 T11002 T11003 arg1Of µl,of
R9701 T11004 T11003 arg2Of lipofectamine,of
R9702 T11005 T10976 arg1Of and,For
R9703 T11005 T10985 arg1Of and,","
R9704 T11007 T11005 arg2Of mixed,and
R9705 T11007 T11006 arg2Of mixed,was
R9706 T11007 T11010 arg1Of mixed,prior
R9707 T11009 T11008 arg1Of minutes,30
R9708 T11010 T11009 arg1Of prior,minutes
R9709 T11010 T11011 arg1Of prior,to
R9710 T11012 T11011 arg2Of stimulation,to
R9711 T11016 T11013 arg1Of Bay11-7082,The
R9712 T11016 T11014 arg1Of Bay11-7082,NF-κB
R9713 T11016 T11015 arg1Of Bay11-7082,inhibitor
R9714 T11016 T11017 arg1Of Bay11-7082,(
R9715 T11016 T11020 arg1Of Bay11-7082,was
R9716 T11016 T11021 arg2Of Bay11-7082,added
R9717 T11018 T11017 arg2Of Invivogen,(
R9718 T11019 T11017 arg3Of ),(
R9719 T11021 T11020 arg2Of added,was
R9720 T11021 T11022 arg1Of added,to
R9721 T11025 T11022 arg2Of hour,to
R9722 T11025 T11023 arg1Of hour,cultures
R9723 T11025 T11024 arg1Of hour,1
R9724 T11025 T11026 arg1Of hour,prior
R9725 T11026 T11027 arg1Of prior,to
R9726 T11028 T11027 arg2Of priming,to
R9727 T11031 T11030 arg1Of priming,no
R9728 T11031 T11032 arg1Of priming,was
R9729 T11031 T11033 arg2Of priming,involved
R9730 T11033 T11029 arg2Of involved,If
R9731 T11033 T11032 arg2Of involved,was
R9732 T11035 T11036 arg1Of inhibitor,was
R9733 T11035 T11037 arg2Of inhibitor,added
R9734 T11037 T11029 arg1Of added,If
R9735 T11037 T11034 arg1Of added,","
R9736 T11037 T11036 arg2Of added,was
R9737 T11039 T11037 arg3Of hour,added
R9738 T11039 T11038 arg1Of hour,1
R9739 T11039 T11040 arg1Of hour,prior
R9740 T11040 T11041 arg1Of prior,to
R9741 T11043 T11041 arg2Of stimulation,to
R9742 T11043 T11042 arg1Of stimulation,inflammasome
R9743 T11047 T11044 arg1Of CFTRinh172,The
R9744 T11047 T11045 arg1Of CFTRinh172,CFTR
R9745 T11047 T11046 arg1Of CFTRinh172,inhibitor
R9746 T11047 T11048 arg1Of CFTRinh172,(
R9747 T11047 T11052 arg1Of CFTRinh172,was
R9748 T11047 T11053 arg2Of CFTRinh172,added
R9749 T11050 T11048 arg2Of Aldrich,(
R9750 T11050 T11049 arg1Of Aldrich,Sigma
R9751 T11051 T11048 arg3Of ),(
R9752 T11053 T11052 arg2Of added,was
R9753 T11053 T11054 arg1Of added,to
R9754 T11053 T11058 arg1Of added,prior
R9755 T11055 T11054 arg2Of cultures,to
R9756 T11057 T11056 arg1Of hours,18
R9757 T11058 T11057 arg1Of prior,hours
R9758 T11058 T11059 arg1Of prior,to
R9759 T11061 T11059 arg2Of stimulation,to
R9760 T11061 T11060 arg1Of stimulation,inflammasome
R9761 T11065 T11062 arg1Of z-YVAD-fmk,The
R9762 T11065 T11063 arg1Of z-YVAD-fmk,caspase-1
R9763 T11065 T11064 arg1Of z-YVAD-fmk,inhibitor
R9764 T11065 T11066 arg1Of z-YVAD-fmk,(
R9765 T11065 T11069 arg1Of z-YVAD-fmk,was
R9766 T11065 T11070 arg2Of z-YVAD-fmk,added
R9767 T11067 T11066 arg2Of Biovision,(
R9768 T11068 T11066 arg3Of ),(
R9769 T11070 T11069 arg2Of added,was
R9770 T11070 T11071 arg1Of added,to
R9771 T11074 T11071 arg2Of hour,to
R9772 T11074 T11072 arg1Of hour,cultures
R9773 T11074 T11073 arg1Of hour,1
R9774 T11074 T11075 arg1Of hour,prior
R9775 T11075 T11076 arg1Of prior,to
R9776 T11078 T11076 arg2Of stimulation,to
R9777 T11078 T11077 arg1Of stimulation,inflammasome
R9778 T11079 T11080 arg1Of Supernatants,were
R9779 T11079 T11081 arg2Of Supernatants,collected
R9780 T11079 T11083 arg2Of Supernatants,stored
R9781 T11081 T11082 arg1Of collected,and
R9782 T11082 T11080 arg2Of and,were
R9783 T11083 T11082 arg2Of stored,and
R9784 T11083 T11084 arg1Of stored,at
R9785 T11086 T11085 arg1Of Cytokines,−20°C.
R9786 T11086 T11087 arg2Of Cytokines,released
R9787 T11086 T11096 arg1Of Cytokines,were
R9788 T11086 T11097 arg2Of Cytokines,quantified
R9789 T11087 T11088 arg1Of released,into
R9790 T11089 T11088 arg2Of supernatants,into
R9791 T11089 T11090 arg1Of supernatants,from
R9792 T11091 T11090 arg2Of PBMCs,from
R9793 T11091 T11092 arg2Of PBMCs,stimulated
R9794 T11092 T11093 arg1Of stimulated,with
R9795 T11095 T11093 arg2Of activators,with
R9796 T11095 T11094 arg1Of activators,inflammasome
R9797 T11097 T11080 modOf quantified,were
R9798 T11097 T11096 arg2Of quantified,were
R9799 T11098 T11097 arg3Of using,quantified
R9800 T11100 T11098 arg2Of ELISA,using
R9801 T11100 T11099 arg1Of ELISA,sandwich
R9802 T11100 T11101 arg1Of ELISA,(
R9803 T11102 T11101 arg2Of eBioscience,(
R9804 T11103 T11101 arg3Of ),(
R10054 T11390 T11389 arg1Of cells,1×106
R10055 T11390 T11391 arg1Of cells,were
R10056 T11390 T11392 arg2Of cells,seeded
R10057 T11390 T11402 arg2Of cells,lysed
R10058 T11392 T11393 arg1Of seeded,in
R10059 T11392 T11401 arg1Of seeded,and
R10060 T11395 T11393 arg2Of plates,in
R10061 T11395 T11394 arg1Of plates,12-well
R10062 T11395 T11396 arg1Of plates,","
R10063 T11395 T11397 arg2Of plates,stimulated
R10064 T11397 T11398 arg1Of stimulated,as
R10065 T11399 T11398 arg2Of indicated,as
R10066 T11401 T11391 arg2Of and,were
R10067 T11401 T11400 arg1Of and,","
R10068 T11402 T11401 arg2Of lysed,and
R10069 T11402 T11403 arg1Of lysed,in
R10070 T11405 T11403 arg2Of buffer,in
R10071 T11405 T11404 arg1Of buffer,RIPA
R10072 T11405 T11406 arg2Of buffer,supplemented
R10073 T11406 T11407 arg1Of supplemented,with
R10074 T11409 T11408 arg1Of protease,Halt
R10075 T11409 T11410 arg1Of protease,and
R10076 T11410 T11407 arg2Of and,with
R10077 T11413 T11410 arg2Of cocktail,and
R10078 T11413 T11411 arg1Of cocktail,phosphatase
R10079 T11413 T11412 arg1Of cocktail,inhibitor
R10080 T11413 T11414 arg1Of cocktail,(
R10081 T11416 T11414 arg2Of Scientific,(
R10082 T11416 T11415 arg1Of Scientific,Thermo
R10083 T11417 T11414 arg3Of ),(
R10084 T11419 T11418 arg1Of concentrations,Protein
R10085 T11419 T11420 arg1Of concentrations,were
R10086 T11419 T11421 arg2Of concentrations,determined
R10087 T11421 T11420 arg2Of determined,were
R10088 T11424 T11421 arg1Of assay,determined
R10089 T11424 T11422 arg2Of assay,by
R10090 T11424 T11423 arg1Of assay,Bradford
R10091 T11424 T11425 arg1Of assay,(
R10092 T11427 T11425 arg2Of Scientific,(
R10093 T11427 T11426 arg1Of Scientific,Thermo
R10094 T11428 T11425 arg3Of ),(
R10095 T11429 T11430 arg1Of Lysates,were
R10096 T11429 T11431 arg2Of Lysates,resolved
R10097 T11429 T11440 arg2Of Lysates,transferred
R10098 T11431 T11432 arg1Of resolved,by
R10099 T11431 T11439 arg1Of resolved,and
R10100 T11433 T11432 arg2Of electrophoreses,by
R10101 T11433 T11434 arg1Of electrophoreses,on
R10102 T11435 T11436 arg1Of 10,%
R10103 T11438 T11434 arg2Of gels,on
R10104 T11438 T11435 arg1Of gels,10
R10105 T11438 T11437 arg1Of gels,SDS-polyacrylamide
R10106 T11439 T11430 arg2Of and,were
R10107 T11440 T11439 arg2Of transferred,and
R10108 T11440 T11441 arg1Of transferred,onto
R10109 T11443 T11441 arg2Of membranes,onto
R10110 T11443 T11442 arg1Of membranes,PVDF
R10111 T11443 T11444 arg1Of membranes,(
R10112 T11445 T11444 arg2Of Millipore,(
R10113 T11446 T11444 arg3Of ),(
R10114 T11447 T11448 arg1Of Blots,were
R10115 T11447 T11449 arg2Of Blots,blocked
R10116 T11447 T11457 arg1Of Blots,probed
R10117 T11449 T11448 arg2Of blocked,were
R10118 T11449 T11450 arg1Of blocked,for
R10119 T11449 T11456 arg1Of blocked,and
R10120 T11452 T11450 arg2Of hour,for
R10121 T11452 T11451 arg1Of hour,1
R10122 T11452 T11453 arg1Of hour,at
R10123 T11455 T11453 arg2Of temperature,at
R10124 T11455 T11454 arg1Of temperature,room
R10125 T11457 T11456 arg2Of probed,and
R10126 T11457 T11458 arg1Of probed,overnight
R10127 T11457 T11459 arg1Of probed,at
R10128 T11460 T11459 arg2Of 4°C,at
R10129 T11460 T11461 arg1Of 4°C,for
R10130 T11462 T11463 arg1Of pro-IL-1β,(
R10131 T11462 T11467 arg1Of pro-IL-1β,","
R10132 T11465 T11463 arg2Of Cruz,(
R10133 T11465 T11464 arg1Of Cruz,Santa
R10134 T11466 T11463 arg3Of ),(
R10135 T11467 T11474 arg1Of ",",or
R10136 T11468 T11467 arg2Of IκBα,","
R10137 T11468 T11469 arg1Of IκBα,(
R10138 T11471 T11469 arg2Of Signaling,(
R10139 T11471 T11470 arg1Of Signaling,Cell
R10140 T11472 T11469 arg3Of ),(
R10141 T11474 T11461 arg2Of or,for
R10142 T11474 T11473 arg1Of or,","
R10143 T11475 T11474 arg2Of β-actin,or
R10144 T11475 T11476 arg1Of β-actin,(
R10145 T11478 T11476 arg2Of Signaling,(
R10146 T11478 T11477 arg1Of Signaling,Cell
R10147 T11479 T11476 arg3Of ),(
R10148 T11480 T11481 arg1Of Blots,were
R10149 T11480 T11483 arg2Of Blots,probed
R10150 T11483 T11481 arg2Of probed,were
R10151 T11483 T11482 arg1Of probed,subsequently
R10152 T11483 T11484 arg1Of probed,with
R10153 T11483 T11497 arg1Of probed,for
R10154 T11487 T11484 arg2Of antibodies,with
R10155 T11487 T11485 arg1Of antibodies,fluorescently-labeled
R10156 T11487 T11486 arg1Of antibodies,secondary
R10157 T11487 T11488 arg1Of antibodies,","
R10158 T11489 T11490 arg1Of IRDye®,680
R10159 T11489 T11491 arg1Of IRDye®,or
R10160 T11491 T11488 arg2Of or,","
R10161 T11492 T11491 arg2Of 800CW,or
R10162 T11492 T11493 arg1Of 800CW,(
R10163 T11495 T11493 arg2Of Biosciences,(
R10164 T11495 T11494 arg1Of Biosciences,LI-COR
R10165 T11496 T11493 arg3Of ),(
R10166 T11499 T11497 arg2Of hour,for
R10167 T11499 T11498 arg1Of hour,1
R10168 T11504 T11501 arg1Of steps,blocking
R10169 T11504 T11502 arg1Of steps,and
R10170 T11504 T11503 arg1Of steps,probing
R10171 T11504 T11505 arg1Of steps,were
R10172 T11504 T11506 arg2Of steps,carried
R10173 T11506 T11500 arg1Of carried,Both
R10174 T11506 T11505 arg2Of carried,were
R10175 T11506 T11507 arg1Of carried,out
R10176 T11506 T11508 arg1Of carried,in
R10177 T11510 T11508 arg2Of saline,in
R10178 T11510 T11509 arg1Of saline,tris-buffered
R10179 T11510 T11511 arg1Of saline,(
R10180 T11510 T11515 arg1Of saline,containing
R10181 T11513 T11511 arg2Of Biosciences,(
R10182 T11513 T11512 arg1Of Biosciences,G
R10183 T11514 T11511 arg3Of ),(
R10184 T11516 T11517 arg1Of 5,%
R10185 T11520 T11516 arg1Of albumin,5
R10186 T11520 T11518 arg1Of albumin,bovine
R10187 T11520 T11519 arg1Of albumin,serum
R10188 T11520 T11521 arg1Of albumin,and
R10189 T11521 T11515 arg2Of and,containing
R10190 T11522 T11523 arg1Of 0.1,%
R10191 T11524 T11521 arg2Of TWEEN,and
R10192 T11524 T11522 arg1Of TWEEN,0.1
R10193 T11524 T11525 arg1Of TWEEN,20
R10194 T11524 T11526 arg1Of TWEEN,(
R10195 T11527 T11526 arg2Of Calbiochem,(
R10196 T11528 T11526 arg3Of ),(
R10197 T11529 T11530 arg1Of Blots,were
R10198 T11529 T11531 arg2Of Blots,imaged
R10199 T11529 T11544 arg2Of Blots,quantified
R10200 T11529 T11545 arg1Of Blots,using
R10201 T11531 T11532 arg1Of imaged,on
R10202 T11531 T11543 arg1Of imaged,and
R10203 T11535 T11532 arg2Of Odyssey,on
R10204 T11535 T11533 arg1Of Odyssey,a
R10205 T11535 T11534 arg1Of Odyssey,LI-COR
R10206 T11538 T11531 arg3Of system,imaged
R10207 T11538 T11536 arg2Of system,infrared
R10208 T11538 T11537 arg1Of system,imaging
R10209 T11538 T11539 arg1Of system,(
R10210 T11541 T11539 arg2Of Biosciences,(
R10211 T11541 T11540 arg1Of Biosciences,LI-COR
R10212 T11542 T11539 arg3Of ),(
R10213 T11543 T11530 arg2Of and,were
R10214 T11544 T11543 arg2Of quantified,and
R10215 T11545 T11544 arg3Of using,quantified
R10216 T11549 T11545 arg2Of software,using
R10217 T11549 T11546 arg1Of software,the
R10218 T11549 T11547 arg2Of software,included
R10219 T11549 T11548 arg1Of software,analysis
R10394 T11763 T11764 arg1Of Quantification,of
R10395 T11766 T11764 arg2Of activity,of
R10396 T11766 T11765 arg1Of activity,caspase-1
R10397 T11769 T11767 arg1Of cells,Bronchial
R10398 T11769 T11768 arg1Of cells,epithelial
R10399 T11769 T11770 arg1Of cells,were
R10400 T11769 T11771 arg2Of cells,plated
R10401 T11771 T11770 arg2Of plated,were
R10402 T11771 T11772 arg1Of plated,in
R10403 T11774 T11772 arg2Of plates,in
R10404 T11774 T11773 arg1Of plates,6-well
R10405 T11775 T11771 arg3Of at,plated
R10406 T11777 T11775 arg2Of cells/well,at
R10407 T11777 T11776 arg1Of cells/well,5×105
R10408 T11777 T11778 arg1Of cells/well,overnight
R10409 T11779 T11780 arg1Of Cells,were
R10410 T11779 T11781 arg2Of Cells,primed
R10411 T11779 T11788 arg2Of Cells,stimulated
R10412 T11779 T11795 arg2Of Cells,stimulated
R10413 T11781 T11782 arg1Of primed,with
R10414 T11781 T11784 arg1Of primed,for
R10415 T11781 T11787 arg1Of primed,and
R10416 T11783 T11782 arg2Of LPS,with
R10417 T11786 T11784 arg2Of hours,for
R10418 T11786 T11785 arg1Of hours,5
R10419 T11787 T11780 arg2Of and,were
R10420 T11788 T11789 arg1Of stimulated,with
R10421 T11788 T11791 arg1Of stimulated,for
R10422 T11788 T11794 arg1Of stimulated,or
R10423 T11790 T11789 arg2Of ATP,with
R10424 T11793 T11791 arg2Of hour,for
R10425 T11793 T11792 arg1Of hour,1
R10426 T11794 T11787 arg2Of or,and
R10427 T11795 T11794 arg2Of stimulated,or
R10428 T11795 T11796 arg1Of stimulated,with
R10429 T11795 T11799 arg1Of stimulated,for
R10430 T11798 T11796 arg2Of PAO1,with
R10431 T11798 T11797 arg1Of PAO1,live
R10432 T11801 T11799 arg2Of hours,for
R10433 T11801 T11800 arg1Of hours,3
R10434 T11802 T11803 arg1Of PBMCs,were
R10435 T11802 T11804 arg2Of PBMCs,stimulated
R10436 T11804 T11803 arg2Of stimulated,were
R10437 T11804 T11807 arg1Of stimulated,day
R10438 T11804 T11808 arg1Of stimulated,as
R10439 T11807 T11805 arg1Of day,the
R10440 T11807 T11806 arg1Of day,same
R10441 T11810 T11808 arg2Of donation,as
R10442 T11810 T11809 arg1Of donation,blood
R10443 T11811 T11812 arg1Of PBMCs,were
R10444 T11811 T11813 arg2Of PBMCs,seeded
R10445 T11811 T11837 arg2Of PBMCs,stimulated
R10446 T11811 T11854 arg2Of PBMCs,stimulated
R10447 T11813 T11814 arg1Of seeded,in
R10448 T11813 T11818 arg1Of seeded,at
R10449 T11813 T11835 arg1Of seeded,and
R10450 T11817 T11814 arg2Of plate,in
R10451 T11817 T11815 arg1Of plate,a
R10452 T11817 T11816 arg1Of plate,96-well
R10453 T11820 T11818 arg2Of density,at
R10454 T11820 T11819 arg1Of density,a
R10455 T11820 T11821 arg1Of density,of
R10456 T11820 T11828 arg1Of density,","
R10457 T11820 T11829 arg2Of density,primed
R10458 T11823 T11821 arg2Of cells/well,of
R10459 T11823 T11822 arg1Of cells/well,4.5×105
R10460 T11823 T11824 arg1Of cells/well,(
R10461 T11826 T11824 arg2Of cells/ml,(
R10462 T11826 T11825 arg1Of cells/ml,2.5×106
R10463 T11827 T11824 arg3Of ),(
R10464 T11829 T11830 arg1Of primed,with
R10465 T11829 T11832 arg1Of primed,for
R10466 T11831 T11830 arg2Of LPS,with
R10467 T11834 T11832 arg2Of hours,for
R10468 T11834 T11833 arg1Of hours,5
R10469 T11835 T11812 arg2Of and,were
R10470 T11837 T11838 arg1Of stimulated,with
R10471 T11837 T11853 arg1Of stimulated,or
R10472 T11839 T11838 arg2Of ATP,with
R10473 T11839 T11840 arg1Of ATP,for
R10474 T11839 T11850 arg1Of ATP,for
R10475 T11842 T11841 arg1Of hour,1
R10476 T11842 T11843 arg1Of hour,or
R10477 T11843 T11840 arg2Of or,for
R10478 T11844 T11843 arg2Of Poly,or
R10479 T11844 T11845 arg1Of Poly,(
R10480 T11846 T11845 arg2Of dA,(
R10481 T11846 T11847 arg1Of dA,:
R10482 T11846 T11848 arg1Of dA,dT
R10483 T11849 T11845 arg3Of ),(
R10484 T11852 T11850 arg2Of hours,for
R10485 T11852 T11851 arg1Of hours,3
R10486 T11853 T11835 arg2Of or,and
R10487 T11853 T11836 arg1Of or,then
R10488 T11854 T11853 arg2Of stimulated,or
R10489 T11854 T11855 arg1Of stimulated,with
R10490 T11854 T11858 arg1Of stimulated,for
R10491 T11857 T11855 arg2Of PAO1,with
R10492 T11857 T11856 arg1Of PAO1,live
R10493 T11860 T11858 arg2Of hours,for
R10494 T11860 T11859 arg1Of hours,3
R10495 T11862 T11861 arg1Of activity,Caspase-1
R10496 T11862 T11863 arg1Of activity,was
R10497 T11862 T11864 arg2Of activity,measured
R10498 T11864 T11863 arg2Of measured,was
R10499 T11865 T11864 arg3Of using,measured
R10500 T11866 T11865 arg2Of FLICA,using
R10501 T11866 T11867 arg1Of FLICA,(
R10502 T11866 T11871 arg1Of FLICA,","
R10503 T11869 T11867 arg2Of Technologies,(
R10504 T11869 T11868 arg1Of Technologies,Immunochemistry
R10505 T11870 T11867 arg3Of ),(
R10506 T11875 T11871 arg2Of probe,","
R10507 T11875 T11872 arg1Of probe,a
R10508 T11875 T11873 arg1Of probe,cell-permeable
R10509 T11875 T11874 arg1Of probe,fluorescent
R10510 T11875 T11876 arg1Of probe,(
R10511 T11875 T11879 arg1Of probe,that
R10512 T11875 T11880 arg1Of probe,binds
R10513 T11877 T11876 arg2Of FAM-YVAD-fmk,(
R10514 T11878 T11876 arg3Of ),(
R10515 T11882 T11880 arg2Of caspase-1,binds
R10516 T11882 T11881 arg1Of caspase-1,active
R10517 T11883 T11884 arg1Of Cells,were
R10518 T11883 T11885 arg2Of Cells,incubated
R10519 T11883 T11893 arg2Of Cells,stained
R10520 T11883 T11902 arg1Of Cells,identify
R10521 T11885 T11890 arg1Of incubated,at
R10522 T11885 T11892 arg1Of incubated,and
R10523 T11887 T11886 arg1Of hour,1
R10524 T11887 T11888 arg1Of hour,with
R10525 T11889 T11888 arg2Of FLICA,with
R10526 T11890 T11887 arg1Of at,hour
R10527 T11891 T11890 arg2Of 37°C,at
R10528 T11892 T11884 arg2Of and,were
R10529 T11893 T11892 arg2Of stained,and
R10530 T11893 T11894 arg1Of stained,with
R10531 T11893 T11901 modOf stained,to
R10532 T11897 T11894 arg2Of antibodies,with
R10533 T11897 T11895 arg1Of antibodies,PE-Cy7-conjugated
R10534 T11897 T11896 arg1Of antibodies,anti-CD14
R10535 T11897 T11898 arg1Of antibodies,(
R10536 T11899 T11898 arg2Of eBioscience,(
R10537 T11900 T11898 arg3Of ),(
R10538 T11902 T11901 arg1Of identify,to
R10539 T11903 T11902 arg2Of monocytes,identify
R10540 T11906 T11904 arg1Of strategy,The
R10541 T11906 T11905 arg1Of strategy,gating
R10542 T11906 T11907 arg1Of strategy,consisted
R10543 T11907 T11908 arg1Of consisted,of
R10544 T11911 T11908 arg2Of cells,of
R10545 T11911 T11909 arg1Of cells,including
R10546 T11911 T11910 arg1Of cells,live
R10547 T11911 T11912 arg1Of cells,that
R10548 T11911 T11913 arg1Of cells,were
R10549 T11911 T11916 arg1Of cells,which
R10550 T11911 T11917 arg1Of cells,were
R10551 T11911 T11919 arg2Of cells,analyzed
R10552 T11914 T11913 arg2Of CD14,were
R10553 T11914 T11915 arg1Of CD14,positive
R10554 T11919 T11917 arg2Of analyzed,were
R10555 T11919 T11918 arg1Of analyzed,subsequently
R10556 T11919 T11920 arg1Of analyzed,for
R10557 T11922 T11920 arg2Of frequency,for
R10558 T11922 T11921 arg1Of frequency,the
R10559 T11922 T11923 arg1Of frequency,of
R10560 T11926 T11923 arg2Of cells,of
R10561 T11926 T11924 arg1Of cells,FLICA
R10562 T11926 T11925 arg1Of cells,positive
R10744 T12145 T12143 arg1Of Assay,NF-κB/AP-1
R10745 T12145 T12144 arg1Of Assay,Activity
R10746 T12146 T12147 arg1Of Supernatants,from
R10747 T12146 T12151 arg1Of Supernatants,were
R10748 T12146 T12152 arg2Of Supernatants,incubated
R10749 T12146 T12162 arg2Of Supernatants,allowed
R10750 T12146 T12164 arg1Of Supernatants,develop
R10751 T12150 T12147 arg2Of cells,from
R10752 T12150 T12148 arg1Of cells,THP-1
R10753 T12150 T12149 arg1Of cells,reporter
R10754 T12152 T12153 arg1Of incubated,with
R10755 T12152 T12161 arg1Of incubated,and
R10756 T12155 T12153 arg2Of substrate,with
R10757 T12155 T12154 arg1Of substrate,Quanti-Blue
R10758 T12155 T12156 arg1Of substrate,(
R10759 T12155 T12159 arg1Of substrate,at
R10760 T12157 T12156 arg2Of Invivogen,(
R10761 T12158 T12156 arg3Of ),(
R10762 T12160 T12159 arg2Of 37°C,at
R10763 T12161 T12151 arg2Of and,were
R10764 T12162 T12161 arg2Of allowed,and
R10765 T12164 T12162 arg3Of develop,allowed
R10766 T12164 T12163 arg1Of develop,to
R10767 T12164 T12165 arg1Of develop,for
R10768 T12167 T12165 arg2Of hours,for
R10769 T12167 T12166 arg1Of hours,16–18
R10770 T12168 T12169 arg1Of Quanti-Blue,contains
R10771 T12169 T12175 arg1Of contains,and
R10772 T12171 T12169 arg2Of substrate,contains
R10773 T12171 T12170 arg1Of substrate,a
R10774 T12171 T12172 arg1Of substrate,for
R10775 T12174 T12172 arg2Of phosphatase,for
R10776 T12174 T12173 arg1Of phosphatase,alkaline
R10777 T12176 T12177 arg1Of changes,in
R10778 T12176 T12183 arg1Of changes,were
R10779 T12176 T12184 arg2Of changes,quantified
R10780 T12179 T12177 arg2Of amount,in
R10781 T12179 T12178 arg1Of amount,the
R10782 T12179 T12180 arg1Of amount,of
R10783 T12182 T12180 arg2Of activity,of
R10784 T12182 T12181 arg1Of activity,NF-κB/AP-1
R10785 T12184 T12175 arg2Of quantified,and
R10786 T12184 T12183 arg2Of quantified,were
R10787 T12184 T12192 modOf quantified,using
R10788 T12187 T12184 arg1Of density,quantified
R10789 T12187 T12185 arg2Of density,by
R10790 T12187 T12186 arg1Of density,optical
R10791 T12187 T12188 arg1Of density,(
R10792 T12187 T12191 arg2Of density,measured
R10793 T12189 T12188 arg2Of λ = 655,(
R10794 T12190 T12188 arg3Of ),(
R10795 T12198 T12193 arg1Of reader,a
R10796 T12198 T12194 arg1Of reader,SpectraMax
R10797 T12198 T12195 arg1Of reader,384
R10798 T12198 T12196 arg1Of reader,Plus
R10799 T12198 T12197 arg1Of reader,plate
R10800 T12198 T12199 arg1Of reader,and
R10801 T12199 T12192 arg2Of and,using
R10802 T12202 T12199 arg2Of software,and
R10803 T12202 T12200 arg1Of software,SoftMax
R10804 T12202 T12201 arg1Of software,Pro
R10805 T12202 T12203 arg1Of software,(
R10806 T12205 T12203 arg2Of Devices,(
R10807 T12205 T12204 arg1Of Devices,Molecular
R10808 T12206 T12203 arg3Of ),(
R10884 T12305 T12304 arg1Of strains,Bacterial
R10885 T12310 T12306 arg1Of PAO1,P.
R10886 T12310 T12307 arg1Of PAO1,aeruginosa
R10887 T12310 T12308 arg1Of PAO1,laboratory
R10888 T12310 T12309 arg1Of PAO1,strains
R10889 T12310 T12311 arg1Of PAO1,and
R10890 T12311 T12315 arg1Of and,were
R10891 T12311 T12316 arg2Of and,obtained
R10892 T12314 T12311 arg2Of mutant,and
R10893 T12314 T12312 arg1Of mutant,the
R10894 T12314 T12313 arg1Of mutant,PAO1ΔexsA
R10895 T12316 T12315 arg2Of obtained,were
R10896 T12316 T12317 arg1Of obtained,from
R10897 T12316 T12345 arg1Of obtained,until
R10898 T12321 T12318 arg1Of P.,Dr.
R10899 T12321 T12319 arg1Of P.,Robert
R10900 T12321 T12320 arg1Of P.,Hancock.
R10901 T12323 T12322 arg1Of strains,aeruginosa
R10902 T12323 T12327 modOf strains,were
R10903 T12324 T12325 arg1Of PAO1,and
R10904 T12325 T12327 arg1Of and,were
R10905 T12325 T12328 arg2Of and,grown
R10906 T12326 T12325 arg2Of PAO1ΔexsA,and
R10907 T12328 T12327 arg2Of grown,were
R10908 T12328 T12329 arg1Of grown,from
R10909 T12331 T12329 arg2Of cultures,from
R10910 T12331 T12330 arg1Of cultures,overnight
R10911 T12331 T12332 arg1Of cultures,in
R10912 T12334 T12332 arg2Of Bertani,in
R10913 T12334 T12333 arg1Of Bertani,Luria
R10914 T12336 T12335 arg2Of LB,(
R10915 T12337 T12335 arg3Of ),(
R10916 T12338 T12335 arg1Of broth,(
R10917 T12338 T12339 arg1Of broth,and
R10918 T12339 T12317 arg2Of and,from
R10919 T12339 T12321 arg1Of and,P.
R10920 T12339 T12323 arg1Of and,strains
R10921 T12340 T12339 arg2Of LB+streptomycin,and
R10922 T12340 T12341 arg1Of LB+streptomycin,(
R10923 T12343 T12341 arg2Of µg/ml,(
R10924 T12343 T12342 arg1Of µg/ml,150
R10925 T12344 T12341 arg3Of ),(
R10926 T12347 T12345 arg2Of phase,until
R10927 T12347 T12346 arg1Of phase,mid-logarithmic
R10928 T12348 T12349 arg1Of Cells,were
R10929 T12348 T12350 arg2Of Cells,washed
R10930 T12348 T12355 arg1Of Cells,resuspended
R10931 T12350 T12349 arg2Of washed,were
R10932 T12350 T12351 arg1Of washed,once
R10933 T12350 T12352 arg1Of washed,in
R10934 T12350 T12354 arg1Of washed,and
R10935 T12353 T12352 arg2Of PBS,in
R10936 T12355 T12354 arg2Of resuspended,and
R10937 T12355 T12356 arg1Of resuspended,in
R10938 T12355 T12358 arg1Of resuspended,to
R10939 T12357 T12356 arg2Of PBS,in
R10940 T12361 T12358 arg2Of density,to
R10941 T12361 T12359 arg1Of density,an
R10942 T12361 T12360 arg1Of density,optical
R10943 T12361 T12362 arg1Of density,of
R10944 T12363 T12362 arg2Of 0.5,of
R10945 T12363 T12364 arg1Of 0.5,(
R10946 T12366 T12364 arg2Of nm,(
R10947 T12366 T12365 arg1Of nm,λ = 600
R10948 T12367 T12364 arg3Of ),(
R10949 T12369 T12368 arg1Of prepare,To
R10950 T12371 T12369 arg2Of bacteria,prepare
R10951 T12371 T12370 arg1Of bacteria,heat-killed
R10952 T12374 T12369 arg1Of PAO1,prepare
R10953 T12374 T12373 arg1Of PAO1,live
R10954 T12374 T12375 arg1Of PAO1,was
R10955 T12374 T12376 arg2Of PAO1,resuspended
R10956 T12374 T12386 arg2Of PAO1,heated
R10957 T12376 T12377 arg1Of resuspended,in
R10958 T12376 T12379 arg1Of resuspended,to
R10959 T12376 T12385 arg1Of resuspended,and
R10960 T12378 T12377 arg2Of PBS,in
R10961 T12382 T12379 arg2Of density,to
R10962 T12382 T12380 arg1Of density,an
R10963 T12382 T12381 arg1Of density,optical
R10964 T12382 T12383 arg1Of density,of
R10965 T12384 T12383 arg2Of 0.5,of
R10966 T12385 T12368 modOf and,To
R10967 T12385 T12372 arg1Of and,","
R10968 T12385 T12375 arg2Of and,was
R10969 T12386 T12385 arg2Of heated,and
R10970 T12386 T12387 arg1Of heated,at
R10971 T12386 T12389 arg1Of heated,for
R10972 T12388 T12387 arg2Of 60°C,at
R10973 T12391 T12389 arg2Of hour,for
R10974 T12391 T12390 arg1Of hour,1
R10975 T12393 T12392 arg2Of stimulations,For
R10976 T12396 T12395 arg1Of PAO1,live
R10977 T12396 T12397 arg1Of PAO1,was
R10978 T12396 T12398 arg2Of PAO1,resuspended
R10979 T12396 T12409 arg2Of PAO1,diluted
R10980 T12398 T12397 arg2Of resuspended,was
R10981 T12398 T12399 arg1Of resuspended,to
R10982 T12398 T12407 arg1Of resuspended,and
R10983 T12399 T12405 arg1Of to,in
R10984 T12402 T12399 arg2Of density,to
R10985 T12402 T12400 arg1Of density,an
R10986 T12402 T12401 arg1Of density,optical
R10987 T12402 T12403 arg1Of density,of
R10988 T12404 T12403 arg2Of 0.5,of
R10989 T12406 T12405 arg2Of PBS,in
R10990 T12407 T12392 arg1Of and,For
R10991 T12407 T12394 arg1Of and,","
R10992 T12409 T12407 arg2Of diluted,and
R10993 T12409 T12408 arg1Of diluted,further
R10994 T12409 T12410 arg1Of diluted,in
R10995 T12412 T12410 arg2Of medium,in
R10996 T12412 T12411 arg1Of medium,culture
R10997 T12412 T12413 arg1Of medium,prior
R10998 T12413 T12414 arg1Of prior,to
R10999 T12415 T12414 arg2Of stimulation,to
R11000 T12415 T12416 modOf stimulation,to
R11001 T12417 T12416 arg1Of achieve,to
R11002 T12420 T12417 arg2Of multiplicity,achieve
R11003 T12420 T12418 arg1Of multiplicity,the
R11004 T12420 T12419 arg2Of multiplicity,desired
R11005 T12420 T12421 arg1Of multiplicity,of
R11006 T12422 T12421 arg2Of infection,of
R11007 T12424 T12423 arg1Of PAO1,Heat-killed
R11008 T12424 T12425 arg1Of PAO1,was
R11009 T12424 T12426 arg2Of PAO1,added
R11010 T12426 T12425 arg2Of added,was
R11011 T12426 T12427 arg1Of added,in
R11012 T12429 T12427 arg2Of volume,in
R11013 T12429 T12428 arg1Of volume,a
R11014 T12429 T12430 arg1Of volume,equivalent
R11015 T12430 T12431 arg1Of equivalent,to
R11016 T12432 T12431 arg2Of that,to
R11017 T12432 T12433 arg2Of that,used
R11018 T12433 T12434 modOf used,to
R11019 T12435 T12434 arg1Of achieve,to
R11020 T12437 T12435 arg2Of MOI,achieve
R11021 T12437 T12436 arg1Of MOI,an
R11022 T12437 T12438 arg1Of MOI,of
R11023 T12437 T12440 arg1Of MOI,for
R11024 T12439 T12438 arg2Of 1,of
R11025 T12442 T12440 arg2Of PAO1,for
R11026 T12442 T12441 arg1Of PAO1,live
R11180 T12611 T12610 arg1Of graphs,All
R11181 T12611 T12612 arg1Of graphs,display
R11182 T12611 T12618 arg1Of graphs,were
R11183 T12611 T12619 arg2Of graphs,generated
R11184 T12612 T12617 arg1Of display,and
R11185 T12616 T12612 arg2Of SEM,display
R11186 T12616 T12613 arg1Of SEM,the
R11187 T12616 T12614 arg1Of SEM,mean
R11188 T12616 T12615 arg1Of SEM,±
R11189 T12619 T12617 arg2Of generated,and
R11190 T12619 T12618 arg2Of generated,were
R11191 T12619 T12620 arg1Of generated,with
R11192 T12621 T12620 arg2Of Prism,with
R11193 T12621 T12622 arg1Of Prism,5
R11194 T12621 T12623 arg1Of Prism,(
R11195 T12624 T12623 arg2Of Graphpad,(
R11196 T12625 T12623 arg3Of ),(
R11197 T12627 T12626 arg1Of significance,Statistical
R11198 T12627 T12628 arg1Of significance,was
R11199 T12627 T12629 arg2Of significance,determined
R11200 T12629 T12628 arg2Of determined,was
R11201 T12629 T12630 arg1Of determined,by
R11202 T12629 T12640 arg1Of determined,where
R11203 T12631 T12630 arg2Of performing,by
R11204 T12635 T12634 arg1Of ANOVA,two-way
R11205 T12635 T12636 arg1Of ANOVA,and
R11206 T12636 T12631 arg2Of and,performing
R11207 T12636 T12632 arg1Of and,one
R11208 T12636 T12633 arg1Of and,or
R11209 T12638 T12637 arg1Of Bonferroni,the
R11210 T12639 T12636 arg2Of post-test,and
R11211 T12639 T12638 arg1Of post-test,Bonferroni
R11212 T12641 T12640 arg2Of applicable,where
R11248 T12684 T12683 arg1Of stimulation,Cell
R11249 T12684 T12685 arg1Of stimulation,and
R11250 T12687 T12685 arg2Of schedule,and
R11251 T12687 T12686 arg1Of schedule,inhibitor
R11252 T12688 T12689 arg1Of Schedule,outlines
R11253 T12689 T12697 arg1Of outlines,in
R11254 T12691 T12689 arg2Of timing,outlines
R11255 T12691 T12690 arg1Of timing,the
R11256 T12691 T12692 arg1Of timing,of
R11257 T12694 T12693 arg1Of addition,inhibitor
R11258 T12694 T12695 arg1Of addition,and
R11259 T12695 T12692 arg2Of and,of
R11260 T12696 T12695 arg2Of priming,and
R11261 T12698 T12697 arg2Of relation,in
R11262 T12698 T12699 arg1Of relation,to
R11263 T12701 T12699 arg2Of stimulation,to
R11264 T12701 T12700 arg1Of stimulation,inflammasome
R11265 T12701 T12702 arg1Of stimulation,(
R11266 T12701 T12705 arg1Of stimulation,for
R11267 T12703 T12702 arg2Of t = 0,(
R11268 T12704 T12702 arg3Of ),(
R11269 T12707 T12706 arg1Of reporter,THP-1
R11270 T12707 T12708 arg1Of reporter,and
R11271 T12708 T12705 arg2Of and,for
R11272 T12712 T12708 arg2Of experiments,and
R11273 T12712 T12709 arg1Of experiments,PBMC
R11274 T12712 T12710 arg1Of experiments,cytokine
R11275 T12712 T12711 arg1Of experiments,quantification
R11276 T12714 T12713 arg1Of treatments,Inhibitor
R11277 T12714 T12715 arg1Of treatments,and
R11278 T12715 T12717 arg1Of and,were
R11279 T12715 T12718 arg2Of and,carried
R11280 T12716 T12715 arg2Of stimulations,and
R11281 T12718 T12717 arg2Of carried,were
R11282 T12718 T12719 arg1Of carried,out
R11283 T12718 T12720 arg1Of carried,as
R11284 T12721 T12720 arg2Of described,as
R11285 T12721 T12722 arg1Of described,in
R11286 T12724 T12725 arg1Of Materials,and
R11287 T12726 T12725 arg2Of Methods,and
R11288 T12727 T12722 arg2Of section,in
R11289 T12727 T12723 arg1Of section,the
R11290 T12727 T12724 arg1Of section,Materials
R11291 T12727 T12726 arg1Of section,Methods
R11356 T12815 T12814 arg1Of epithelial,Airway
R11357 T12816 T12815 arg1Of cells,epithelial
R11358 T12816 T12817 arg1Of cells,do
R11359 T12816 T12820 arg1Of cells,contribute
R11360 T12820 T12817 arg2Of contribute,do
R11361 T12820 T12818 arg1Of contribute,not
R11362 T12820 T12819 arg1Of contribute,significantly
R11363 T12820 T12821 arg1Of contribute,to
R11364 T12820 T12824 arg1Of contribute,in
R11365 T12823 T12821 arg2Of production,to
R11366 T12823 T12822 arg1Of production,IL-1β
R11367 T12825 T12824 arg2Of response,in
R11368 T12825 T12826 arg1Of response,to
R11369 T12828 T12826 arg2Of stimuli,to
R11370 T12828 T12827 arg1Of stimuli,inflammasome
R11371 T12831 T12829 arg1Of lines,Control
R11372 T12831 T12830 arg1Of lines,cell
R11373 T12831 T12832 arg1Of lines,(
R11374 T12831 T12843 arg1Of lines,and
R11375 T12834 T12833 arg2Of A,(
R11376 T12835 T12833 arg3Of ),(
R11377 T12836 T12832 arg2Of S9,(
R11378 T12836 T12833 arg1Of S9,(
R11379 T12836 T12837 arg1Of S9,","
R11380 T12839 T12838 arg2Of C,(
R11381 T12840 T12838 arg3Of ),(
R11382 T12841 T12837 arg2Of NuLi-1,","
R11383 T12841 T12838 arg1Of NuLi-1,(
R11384 T12842 T12832 arg3Of ),(
R11385 T12848 T12843 arg2Of lines,and
R11386 T12848 T12844 arg1Of lines,their
R11387 T12848 T12845 arg1Of lines,corresponding
R11388 T12848 T12846 arg1Of lines,CF
R11389 T12848 T12847 arg1Of lines,cell
R11390 T12848 T12849 arg1Of lines,(
R11391 T12848 T12862 modOf lines,were
R11392 T12851 T12850 arg2Of B,(
R11393 T12852 T12850 arg3Of ),(
R11394 T12853 T12855 arg1Of IB3-1,and
R11395 T12855 T12849 arg2Of and,(
R11396 T12855 T12850 arg1Of and,(
R11397 T12855 T12854 arg1Of and,","
R11398 T12857 T12856 arg2Of D,(
R11399 T12858 T12856 arg3Of ),(
R11400 T12859 T12855 arg2Of CuFi-1,and
R11401 T12859 T12856 arg1Of CuFi-1,(
R11402 T12860 T12849 arg3Of ),(
R11403 T12861 T12862 arg1Of cells,were
R11404 T12861 T12863 arg2Of cells,stimulated
R11405 T12863 T12862 arg2Of stimulated,were
R11406 T12863 T12864 arg1Of stimulated,with
R11407 T12866 T12865 arg1Of aeruginosa,P.
R11408 T12866 T12867 arg1Of aeruginosa,(
R11409 T12866 T12870 arg1Of aeruginosa,","
R11410 T12868 T12867 arg2Of MOI = 10,(
R11411 T12869 T12867 arg3Of ),(
R11412 T12870 T12877 arg1Of ",",or
R11413 T12871 T12870 arg2Of ATP,","
R11414 T12871 T12872 arg1Of ATP,(
R11415 T12874 T12872 arg2Of mM,(
R11416 T12874 T12873 arg1Of mM,5
R11417 T12875 T12872 arg3Of ),(
R11418 T12877 T12864 arg2Of or,with
R11419 T12877 T12876 arg1Of or,","
R11420 T12877 T12883 arg1Of or,","
R11421 T12877 T12884 arg1Of or,for
R11422 T12878 T12877 arg2Of IL-1β,or
R11423 T12878 T12879 arg1Of IL-1β,(
R11424 T12881 T12879 arg2Of ng/ml,(
R11425 T12881 T12880 arg1Of ng/ml,10
R11426 T12882 T12879 arg3Of ),(
R11427 T12887 T12884 arg2Of times,for
R11428 T12887 T12885 arg1Of times,the
R11429 T12887 T12886 arg2Of times,indicated
R11430 T12887 T12888 arg1Of times,(
R11431 T12891 T12888 arg2Of experiments,(
R11432 T12891 T12889 arg1Of experiments,n = 3
R11433 T12891 T12890 arg1Of experiments,individual
R11434 T12892 T12888 arg3Of ),(
R11435 T12893 T12894 arg1Of Cells,were
R11436 T12893 T12895 arg2Of Cells,primed
R11437 T12895 T12894 arg2Of primed,were
R11438 T12895 T12896 arg1Of primed,with
R11439 T12895 T12902 arg1Of primed,for
R11440 T12895 T12905 arg1Of primed,where
R11441 T12897 T12896 arg2Of LPS,with
R11442 T12897 T12898 arg1Of LPS,(
R11443 T12900 T12898 arg2Of ng/ml,(
R11444 T12900 T12899 arg1Of ng/ml,100
R11445 T12901 T12898 arg3Of ),(
R11446 T12904 T12902 arg2Of hours,for
R11447 T12904 T12903 arg1Of hours,4
R11448 T12906 T12905 arg2Of appropriate,where
R11449 T12909 T12907 arg1Of supernatants,Cell
R11450 T12909 T12908 arg1Of supernatants,culture
R11451 T12909 T12910 arg1Of supernatants,were
R11452 T12909 T12911 arg2Of supernatants,assayed
R11453 T12911 T12910 arg2Of assayed,were
R11454 T12911 T12912 arg1Of assayed,for
R11455 T12913 T12914 arg1Of IL-1β,and
R11456 T12915 T12914 arg2Of IL-8,and
R11457 T12916 T12912 arg2Of production,for
R11458 T12916 T12913 arg1Of production,IL-1β
R11459 T12916 T12915 arg1Of production,IL-8
R11460 T12916 T12917 arg1Of production,by
R11461 T12918 T12917 arg2Of ELISA,by
R11462 T12919 T12920 arg1Of Insert,shows
R11463 T12920 T12923 arg1Of shows,in
R11464 T12922 T12920 arg2Of secretion,shows
R11465 T12922 T12921 arg1Of secretion,IL-8
R11466 T12924 T12923 arg2Of response,in
R11467 T12924 T12925 arg1Of response,to
R11468 T12926 T12925 arg2Of stimulation,to
R11469 T12926 T12927 arg1Of stimulation,with
R11470 T12928 T12927 arg2Of IL-1β,with
R11471 T12928 T12929 arg1Of IL-1β,(
R11472 T12931 T12929 arg2Of ng/ml,(
R11473 T12931 T12930 arg1Of ng/ml,10
R11474 T12932 T12929 arg3Of ),(
R11618 T13122 T13121 arg1Of cells,Airway
R11619 T13122 T13123 arg1Of cells,do
R11620 T13122 T13126 arg1Of cells,upregulate
R11621 T13126 T13123 arg2Of upregulate,do
R11622 T13126 T13124 arg1Of upregulate,not
R11623 T13126 T13125 arg1Of upregulate,strongly
R11624 T13126 T13129 arg1Of upregulate,in
R11625 T13128 T13126 arg2Of activity,upregulate
R11626 T13128 T13127 arg1Of activity,caspase-1
R11627 T13130 T13129 arg2Of response,in
R11628 T13130 T13131 arg1Of response,to
R11629 T13133 T13131 arg2Of stimuli,to
R11630 T13133 T13132 arg1Of stimuli,inflammasome
R11631 T13134 T13135 arg1Of S9,and
R11632 T13136 T13135 arg2Of IB3-1,and
R11633 T13137 T13134 arg1Of cells,S9
R11634 T13137 T13136 arg1Of cells,IB3-1
R11635 T13137 T13138 arg1Of cells,were
R11636 T13137 T13139 arg2Of cells,examined
R11637 T13139 T13138 arg2Of examined,were
R11638 T13139 T13140 arg1Of examined,for
R11639 T13139 T13143 arg1Of examined,following
R11640 T13142 T13140 arg2Of activation,for
R11641 T13142 T13141 arg1Of activation,caspase-1
R11642 T13145 T13143 arg2Of stimulation,following
R11643 T13145 T13144 arg1Of stimulation,inflammasome
R11644 T13145 T13146 arg1Of stimulation,with
R11645 T13148 T13147 arg1Of aeruginosa,P.
R11646 T13148 T13149 arg1Of aeruginosa,(
R11647 T13148 T13152 arg1Of aeruginosa,and
R11648 T13150 T13149 arg2Of MOI = 10,(
R11649 T13151 T13149 arg3Of ),(
R11650 T13152 T13146 arg2Of and,with
R11651 T13153 T13152 arg2Of ATP,and
R11652 T13153 T13154 arg1Of ATP,(
R11653 T13156 T13154 arg2Of mM,(
R11654 T13156 T13155 arg1Of mM,5
R11655 T13157 T13154 arg3Of ),(
R11656 T13158 T13159 arg1Of Cells,were
R11657 T13158 T13160 arg2Of Cells,primed
R11658 T13160 T13159 arg2Of primed,were
R11659 T13160 T13161 arg1Of primed,with
R11660 T13160 T13163 arg1Of primed,for
R11737 T13236 T13231 arg2Of and,were
R11661 T13160 T13166 arg1Of primed,where
R11662 T13162 T13161 arg2Of LPS,with
R11663 T13165 T13163 arg2Of hours,for
R11664 T13165 T13164 arg1Of hours,5
R11665 T13167 T13166 arg2Of appropriate,where
R11666 T13170 T13168 arg1Of histogram,A
R11667 T13170 T13169 arg1Of histogram,representative
R11668 T13170 T13171 arg1Of histogram,of
R11669 T13170 T13175 arg1Of histogram,is
R11670 T13170 T13176 arg2Of histogram,shown
R11671 T13174 T13171 arg2Of cells,of
R11672 T13174 T13172 arg1Of cells,%
R11673 T13174 T13173 arg1Of cells,caspase-1-active
R11674 T13176 T13175 arg2Of shown,is
R11675 T13176 T13177 arg1Of shown,in
R11676 T13176 T13181 arg1Of shown,and
R11677 T13179 T13177 arg2Of A,in
R11678 T13179 T13178 arg2Of A,(
R11679 T13180 T13178 arg3Of ),(
R11680 T13184 T13182 arg1Of values,the
R11681 T13184 T13183 arg1Of values,averaged
R11682 T13184 T13185 arg1Of values,are
R11683 T13184 T13186 arg2Of values,shown
R11684 T13186 T13181 arg2Of shown,and
R11685 T13186 T13185 arg2Of shown,are
R11686 T13186 T13187 arg1Of shown,in
R11687 T13189 T13187 arg2Of B,in
R11688 T13189 T13188 arg2Of B,(
R11689 T13189 T13191 arg1Of B,(
R11690 T13189 T13205 modOf B,well
R11691 T13189 T13219 arg1Of B,D-fmk
R11692 T13190 T13188 arg3Of ),(
R11693 T13194 T13191 arg2Of experiments,(
R11694 T13194 T13192 arg1Of experiments,n = 3
R11695 T13194 T13193 arg1Of experiments,separate
R11696 T13195 T13191 arg3Of ),(
R11697 T13197 T13196 arg2Of (,.
R11698 T13198 T13196 arg3Of C,.
R11699 T13200 T13196 arg1Of -1 ce,.
R11700 T13200 T13199 arg1Of -1 ce,) IB3
R11701 T13200 T13201 arg1Of -1 ce,l
R11702 T13200 T13205 arg1Of -1 ce,well
R11703 T13200 T13206 arg2Of -1 ce,) were pre-
R11704 T13203 T13201 arg2Of ×104 cells,l
R11705 T13203 T13202 arg1Of ×104 cells,ls (5
R11706 T13204 T13201 arg3Of /,l
R11707 T13206 T13205 arg2Of ) were pre-,well
R11708 T13206 T13207 arg1Of ) were pre-,tre
R11709 T13209 T13207 arg2Of ted ,tre
R11710 T13209 T13208 arg1Of ted ,a
R11711 T13209 T13210 arg1Of ted ,for
R11712 T13212 T13210 arg2Of h increasing c,for
R11713 T13212 T13211 arg1Of h increasing c,1 hour wit
R11714 T13212 T13213 arg1Of h increasing c,on
R11715 T13214 T13213 arg2Of centration,on
R11716 T13214 T13215 arg1Of centration,s
R11717 T13217 T13215 arg2Of YV,s
R11718 T13217 T13216 arg1Of YV,of z-
R11719 T13218 T13215 arg3Of A,s
R11720 T13219 T13220 arg1Of D-fmk,(1
R11721 T13221 T13220 arg2Of 0–30 µM) pr,(1
R11722 T13221 T13222 arg1Of 0–30 µM) pr,ior
R11723 T13224 T13222 arg2Of stimulatio,ior
R11724 T13224 T13223 arg1Of stimulatio,to
R11725 T13224 T13225 arg1Of stimulatio,n
R11726 T13226 T13225 arg2Of with P. ,n
R11727 T13227 T13225 arg3Of a,n
R11728 T13230 T13228 arg1Of supernatants,Cell
R11729 T13230 T13229 arg1Of supernatants,culture
R11730 T13230 T13231 arg1Of supernatants,were
R11731 T13230 T13232 arg2Of supernatants,collected
R11732 T13230 T13237 arg2Of supernatants,assayed
R11733 T13232 T13233 arg1Of collected,after
R11734 T13232 T13236 arg1Of collected,and
R11735 T13235 T13233 arg2Of hours,after
R11736 T13235 T13234 arg1Of hours,6
R11738 T13237 T13236 arg2Of assayed,and
R11739 T13237 T13238 arg1Of assayed,for
R11740 T13239 T13238 arg2Of IL-6,for
R11741 T13241 T13237 arg1Of ELISA,assayed
R11742 T13241 T13240 arg2Of ELISA,by
R11743 T13241 T13242 arg1Of ELISA,(
R11744 T13243 T13242 arg2Of n = 3,(
R11745 T13244 T13242 arg3Of ),(
R11888 T13410 T13411 arg1Of PBMCs,from
R11889 T13410 T13416 arg1Of PBMCs,show
R11890 T13413 T13412 arg1Of patients,CF
R11891 T13413 T13414 arg1Of patients,and
R11892 T13414 T13411 arg2Of and,from
R11893 T13415 T13414 arg2Of controls,and
R11894 T13418 T13416 arg2Of increases,show
R11895 T13418 T13417 arg1Of increases,similar
R11896 T13418 T13419 arg1Of increases,in
R11897 T13421 T13419 arg2Of activity,in
R11898 T13421 T13420 arg1Of activity,caspase-1
R11899 T13421 T13422 arg1Of activity,upon
R11900 T13424 T13422 arg2Of activation,upon
R11901 T13424 T13423 arg1Of activation,inflammasome
R11902 T13425 T13426 arg1Of PBMCs,from
R11903 T13425 T13438 arg1Of PBMCs,were
R11904 T13425 T13439 arg2Of PBMCs,primed
R11905 T13428 T13427 arg1Of patients,CF
R11906 T13428 T13429 arg1Of patients,(
R11907 T13428 T13432 arg1Of patients,and
R11908 T13430 T13429 arg2Of n = 6,(
R11909 T13431 T13429 arg3Of ),(
R11910 T13432 T13426 arg2Of and,from
R11911 T13434 T13432 arg2Of controls,and
R11912 T13434 T13433 arg1Of controls,healthy
R11913 T13434 T13435 arg1Of controls,(
R11914 T13436 T13435 arg2Of n = 6,(
R11915 T13437 T13435 arg3Of ),(
R11916 T13439 T13438 arg2Of primed,were
R11917 T13439 T13440 arg1Of primed,with
R11918 T13439 T13446 arg1Of primed,for
R11919 T13439 T13472 arg1Of primed,for
R11920 T13441 T13440 arg2Of LPS,with
R11921 T13441 T13442 arg1Of LPS,(
R11922 T13444 T13442 arg2Of ng/ml,(
R11923 T13444 T13443 arg1Of ng/ml,10
R11924 T13445 T13442 arg3Of ),(
R11925 T13448 T13446 arg2Of hours,for
R11926 T13448 T13447 arg1Of hours,5
R11927 T13449 T13450 arg1Of prior,to
R11928 T13451 T13452 arg1Of stimulation,with
R11929 T13451 T13461 arg1Of stimulation,or
R11930 T13453 T13452 arg2Of ATP,with
R11931 T13453 T13454 arg1Of ATP,(
R11932 T13453 T13458 arg1Of ATP,for
R11933 T13456 T13454 arg2Of mM,(
R11934 T13456 T13455 arg1Of mM,5
R11935 T13457 T13454 arg3Of ),(
R11936 T13460 T13458 arg2Of hour,for
R11937 T13460 T13459 arg1Of hour,1
R11938 T13461 T13450 arg2Of or,to
R11939 T13462 T13461 arg2Of Poly,or
R11940 T13462 T13463 arg1Of Poly,(
R11941 T13462 T13468 arg1Of Poly,(
R11942 T13464 T13463 arg2Of dA,(
R11943 T13464 T13465 arg1Of dA,:
R11944 T13464 T13466 arg1Of dA,dT
R11945 T13467 T13463 arg3Of ),(
R11946 T13470 T13468 arg2Of µg/ml,(
R11947 T13470 T13469 arg1Of µg/ml,1
R11948 T13471 T13468 arg3Of ),(
R11949 T13472 T13449 arg1Of for,prior
R11950 T13474 T13472 arg2Of hours,for
R11951 T13474 T13473 arg1Of hours,3
R11952 T13475 T13476 arg1Of PBMCs,were
R11953 T13475 T13477 arg2Of PBMCs,stimulated
R11954 T13477 T13476 arg2Of stimulated,were
R11955 T13477 T13478 arg1Of stimulated,with
R11956 T13482 T13478 arg2Of PAO1,with
R11957 T13482 T13479 arg1Of PAO1,P.
R11958 T13482 T13480 arg1Of PAO1,aeruginosa
R11959 T13482 T13481 arg1Of PAO1,strain
R11960 T13482 T13483 arg1Of PAO1,for
R11961 T13485 T13483 arg2Of hours,for
R11962 T13485 T13484 arg1Of hours,3
R11963 T13488 T13486 arg1Of histogram,A
R11964 T13488 T13487 arg1Of histogram,representative
R11965 T13488 T13489 arg1Of histogram,of
R11966 T13488 T13494 arg1Of histogram,is
R11967 T13488 T13495 arg2Of histogram,shown
R11968 T13493 T13489 arg2Of cells,of
R11969 T13493 T13490 arg1Of cells,the
R11970 T13493 T13491 arg1Of cells,%
R11971 T13493 T13492 arg1Of cells,caspase-1-active
R11972 T13495 T13494 arg2Of shown,is
R11973 T13495 T13496 arg1Of shown,in
R11974 T13498 T13496 arg2Of A,in
R11975 T13498 T13497 arg2Of A,(
R11976 T13498 T13500 arg1Of A,with
R11977 T13499 T13497 arg3Of ),(
R11978 T13503 T13500 arg2Of values,with
R11979 T13503 T13501 arg1Of values,the
R11980 T13503 T13502 arg1Of values,averaged
R11981 T13503 T13504 arg2Of values,shown
R11982 T13504 T13505 arg1Of shown,in
R11983 T13507 T13505 arg2Of B,in
R11984 T13507 T13506 arg2Of B,(
R11985 T13508 T13506 arg3Of ),(
R12104 T13645 T13651 arg1Of PBMCs,produce
R12105 T13645 T13646 arg1Of PBMCs,from
R12106 T13645 T13649 arg1Of PBMCs,do
R12107 T13648 T13646 arg2Of patients,from
R12108 T13648 T13647 arg1Of patients,CF
R12109 T13651 T13650 arg1Of produce,not
R12110 T13651 T13649 arg2Of produce,do
R12111 T13653 T13651 arg2Of amounts,produce
R12112 T13653 T13652 arg2Of amounts,increased
R12113 T13653 T13654 arg1Of amounts,of
R12114 T13655 T13654 arg2Of IL-1β,of
R12115 T13656 T13657 arg1Of PBMCs,from
R12116 T13656 T13703 arg1Of PBMCs,or
R12117 T13658 T13669 modOf CF,were
R12118 T13659 T13660 arg1Of patients,(
R12119 T13659 T13663 arg1Of patients,and
R12120 T13661 T13660 arg2Of n = 17–20,(
R12121 T13662 T13660 arg3Of ),(
R12122 T13663 T13669 arg1Of and,were
R12123 T13663 T13670 arg2Of and,primed
R12124 T13665 T13663 arg2Of controls,and
R12125 T13665 T13664 arg1Of controls,healthy
R12126 T13665 T13666 arg1Of controls,(
R12127 T13667 T13666 arg2Of n = 15–19,(
R12128 T13668 T13666 arg3Of ),(
R12129 T13670 T13669 arg2Of primed,were
R12130 T13670 T13671 arg1Of primed,with
R12131 T13672 T13671 arg2Of LPS,with
R12132 T13672 T13673 arg1Of LPS,(
R12133 T13675 T13673 arg2Of ng/ml,(
R12134 T13675 T13674 arg1Of ng/ml,10
R12135 T13676 T13673 arg3Of ),(
R12136 T13677 T13678 arg1Of overnight,and
R12137 T13678 T13687 arg1Of and,","
R12138 T13679 T13678 arg2Of stimulated,and
R12139 T13679 T13680 arg1Of stimulated,with
R12140 T13683 T13680 arg2Of PAO1,with
R12141 T13683 T13681 arg1Of PAO1,P.
R12142 T13683 T13682 arg1Of PAO1,aeruginosa
R12143 T13683 T13684 arg1Of PAO1,(
R12144 T13685 T13684 arg2Of MOI = 1,(
R12145 T13686 T13684 arg3Of ),(
R12146 T13690 T13658 arg1Of PAO1,CF
R12147 T13690 T13677 arg1Of PAO1,overnight
R12148 T13690 T13679 arg2Of PAO1,stimulated
R12149 T13690 T13688 arg1Of PAO1,P.
R12150 T13690 T13689 arg1Of PAO1,aeruginosa
R12151 T13690 T13691 arg1Of PAO1,lacking
R12152 T13690 T13696 arg1Of PAO1,","
R12153 T13692 T13691 arg2Of exsA,lacking
R12154 T13692 T13693 arg1Of exsA,(
R12155 T13694 T13693 arg2Of MOI = 1,(
R12156 T13695 T13693 arg3Of ),(
R12157 T13696 T13657 arg2Of ",",from
R12158 T13697 T13696 arg2Of ATP,","
R12159 T13697 T13698 arg1Of ATP,(
R12160 T13700 T13698 arg2Of mM,(
R12161 T13700 T13699 arg1Of mM,5
R12162 T13701 T13698 arg3Of ),(
R12163 T13703 T13702 arg1Of or,","
R12164 T13703 T13714 arg1Of or,for
R12165 T13704 T13703 arg2Of Poly,or
R12166 T13704 T13705 arg1Of Poly,(
R12167 T13704 T13710 arg1Of Poly,(
R12168 T13706 T13705 arg2Of dA,(
R12169 T13706 T13707 arg1Of dA,:
R12170 T13706 T13708 arg1Of dA,dT
R12171 T13709 T13705 arg3Of ),(
R12172 T13712 T13710 arg2Of µg/ml,(
R12173 T13712 T13711 arg1Of µg/ml,1
R12174 T13713 T13710 arg3Of ),(
R12175 T13716 T13714 arg2Of hours,for
R12176 T13716 T13715 arg1Of hours,24
R12177 T13718 T13717 arg1Of aeruginosa,P.
R12178 T13718 T13719 arg1Of aeruginosa,lacking
R12179 T13718 T13721 arg1Of aeruginosa,was
R12180 T13718 T13722 arg2Of aeruginosa,used
R12181 T13720 T13719 arg2Of exsA,lacking
R12182 T13722 T13721 arg2Of used,was
R12183 T13722 T13723 arg1Of used,as
R12184 T13722 T13729 arg1Of used,in
R12185 T13728 T13723 arg2Of control,as
R12186 T13728 T13724 arg1Of control,a
R12187 T13728 T13725 arg1Of control,type
R12188 T13728 T13726 arg1Of control,III
R12189 T13728 T13727 arg1Of control,secretion
R12190 T13730 T13729 arg2Of comparison,in
R12191 T13730 T13731 arg1Of comparison,with
R12192 T13734 T13731 arg2Of aeruginosa,with
R12193 T13734 T13732 arg1Of aeruginosa,wild-type
R12194 T13734 T13733 arg1Of aeruginosa,P.
R12195 T13735 T13736 arg1Of Supernatants,were
R12196 T13735 T13737 arg2Of Supernatants,assayed
R12197 T13737 T13736 arg2Of assayed,were
R12198 T13737 T13738 arg1Of assayed,for
R12199 T13740 T13739 arg2Of A,(
R12200 T13741 T13739 arg3Of ),(
R12201 T13742 T13739 arg1Of IL-1β,(
R12202 T13742 T13743 arg1Of IL-1β,and
R12203 T13743 T13738 arg2Of and,for
R12204 T13745 T13744 arg2Of B,(
R12205 T13746 T13744 arg3Of ),(
R12206 T13747 T13743 arg2Of IL-8,and
R12207 T13747 T13744 arg1Of IL-8,(
R12360 T13943 T13942 arg1Of activation,NF-κB
R12361 T13943 T13944 arg1Of activation,potentiates
R12362 T13946 T13944 arg2Of degree,potentiates
R12363 T13946 T13945 arg1Of degree,the
R12364 T13946 T13947 arg1Of degree,of
R12365 T13946 T13952 arg1Of degree,upon
R12366 T13949 T13950 arg1Of production,and
R12367 T13950 T13947 arg2Of and,of
R12368 T13950 T13948 arg1Of and,IL-1β
R12369 T13951 T13950 arg2Of secretion,and
R12370 T13954 T13952 arg2Of activation,upon
R12371 T13954 T13953 arg1Of activation,inflammasome
R12372 T13957 T13955 arg1Of cells,THP-1
R12373 T13957 T13956 arg1Of cells,reporter
R12374 T13957 T13958 arg1Of cells,were
R12375 T13957 T13959 arg2Of cells,primed
R12376 T13957 T13966 arg1Of cells,stimulated
R12377 T13959 T13958 arg2Of primed,were
R12378 T13959 T13960 arg1Of primed,overnight
R12379 T13959 T13961 arg1Of primed,with
R12380 T13959 T13965 arg1Of primed,and
R12381 T13964 T13961 arg2Of aeruginosa,with
R12382 T13964 T13962 arg1Of aeruginosa,heat-killed
R12383 T13964 T13963 arg1Of aeruginosa,P.
R12384 T13965 T13982 arg2Of and,indicated
R12385 T13966 T13965 arg2Of stimulated,and
R12386 T13966 T13970 arg1Of stimulated,with
R12387 T13969 T13966 arg2Of day,stimulated
R12388 T13969 T13967 arg1Of day,the
R12389 T13969 T13968 arg1Of day,next
R12390 T13973 T13970 arg2Of aeruginosa,with
R12391 T13973 T13971 arg1Of aeruginosa,live
R12392 T13973 T13972 arg1Of aeruginosa,P.
R12393 T13978 T13974 arg1Of aeruginosa,or
R12394 T13978 T13975 arg1Of aeruginosa,additional
R12395 T13978 T13976 arg1Of aeruginosa,heat-killed
R12396 T13978 T13977 arg1Of aeruginosa,P.
R12397 T13978 T13979 arg1Of aeruginosa,for
R12398 T13978 T13982 arg1Of aeruginosa,indicated
R12399 T13981 T13979 arg2Of times,for
R12400 T13981 T13980 arg1Of times,the
R12401 T13985 T13983 arg1Of supernatants,Cell
R12402 T13985 T13984 arg1Of supernatants,culture
R12403 T13985 T13986 arg1Of supernatants,were
R12404 T13985 T13987 arg2Of supernatants,assayed
R12405 T13987 T13986 arg2Of assayed,were
R12406 T13987 T13988 arg1Of assayed,for
R12407 T13990 T13989 arg2Of A,(
R12408 T13991 T13989 arg3Of ),(
R12409 T13993 T13989 arg1Of activity,(
R12410 T13993 T13992 arg1Of activity,NF-κB/AP-1
R12411 T13993 T13994 arg1Of activity,","
R12412 T13994 T14000 arg1Of ",",and
R12413 T13996 T13995 arg2Of B,(
R12414 T13997 T13995 arg3Of ),(
R12415 T13998 T13994 arg2Of IL-8,","
R12416 T13998 T13995 arg1Of IL-8,(
R12417 T14000 T13988 arg2Of and,for
R12418 T14000 T13999 arg1Of and,","
R12419 T14002 T14001 arg2Of C,(
R12420 T14003 T14001 arg3Of ),(
R12421 T14005 T14000 arg2Of secretion,and
R12422 T14005 T14001 arg1Of secretion,(
R12423 T14005 T14004 arg1Of secretion,IL-1β
R12424 T14005 T14006 arg1Of secretion,(
R12425 T14008 T14006 arg2Of experiments,(
R12426 T14008 T14007 arg1Of experiments,n = 3–6
R12427 T14009 T14006 arg3Of ),(
R12428 T14014 T14010 arg2Of method,Using
R12429 T14014 T14011 arg1Of method,the
R12430 T14014 T14012 arg1Of method,same
R12431 T14014 T14013 arg1Of method,stimulation
R12432 T14018 T14016 arg1Of cells,THP-1
R12433 T14018 T14017 arg1Of cells,reporter
R12434 T14018 T14019 arg1Of cells,were
R12435 T14018 T14020 arg2Of cells,treated
R12436 T14020 T14010 modOf treated,Using
R12437 T14020 T14015 arg1Of treated,","
R12438 T14020 T14019 arg2Of treated,were
R12439 T14020 T14021 arg1Of treated,with
R12440 T14020 T14027 arg1Of treated,for
R12441 T14022 T14021 arg2Of Bay11-7082,with
R12442 T14022 T14023 arg1Of Bay11-7082,(
R12443 T14025 T14023 arg2Of µM,(
R12444 T14025 T14024 arg1Of µM,20
R12445 T14026 T14023 arg3Of ),(
R12446 T14029 T14027 arg2Of hour,for
R12447 T14029 T14028 arg1Of hour,1
R12448 T14029 T14030 arg1Of hour,prior
R12449 T14030 T14031 arg1Of prior,to
R12450 T14032 T14031 arg2Of priming,to
R12451 T14032 T14033 arg1Of priming,with
R12452 T14035 T14034 arg1Of PAO1,heat-killed
R12453 T14035 T14036 arg1Of PAO1,or
R12454 T14036 T14033 arg2Of or,with
R12455 T14038 T14036 arg2Of PAO1,or
R12456 T14038 T14037 arg1Of PAO1,live
R12457 T14039 T14040 arg1Of Supernatants,were
R12458 T14039 T14041 arg2Of Supernatants,assayed
R12459 T14041 T14040 arg2Of assayed,were
R12460 T14041 T14042 arg1Of assayed,at
R12461 T14041 T14045 arg1Of assayed,for
R12462 T14044 T14042 arg2Of hours,at
R12463 T14044 T14043 arg1Of hours,24
R12464 T14047 T14046 arg2Of D,(
R12465 T14048 T14046 arg3Of ),(
R12466 T14050 T14046 arg1Of activity,(
R12467 T14050 T14049 arg1Of activity,NF-κB/AP-1
R12468 T14050 T14051 arg1Of activity,","
R12469 T14051 T14057 arg1Of ",",and
R12470 T14053 T14052 arg2Of E,(
R12471 T14054 T14052 arg3Of ),(
R12472 T14055 T14051 arg2Of IL-8,","
R12473 T14055 T14052 arg1Of IL-8,(
R12474 T14057 T14045 arg2Of and,for
R12475 T14057 T14056 arg1Of and,","
R12476 T14059 T14058 arg2Of F,(
R12477 T14060 T14058 arg3Of ),(
R12478 T14062 T14057 arg2Of secretion,and
R12479 T14062 T14058 arg1Of secretion,(
R12480 T14062 T14061 arg1Of secretion,IL-1β
R12481 T14062 T14063 arg1Of secretion,(
R12482 T14064 T14063 arg2Of n = 3–5,(
R12483 T14065 T14063 arg3Of ),(
R12484 T14066 T14067 arg1Of PBMCs,from
R12485 T14066 T14078 arg1Of PBMCs,were
R12486 T14066 T14079 arg2Of PBMCs,treated
R12487 T14066 T14093 arg2Of PBMCs,stimulated
R12488 T14069 T14068 arg1Of patients,CF
R12489 T14069 T14070 arg1Of patients,(
R12490 T14069 T14073 arg1Of patients,and
R12491 T14071 T14070 arg2Of n = 11–15,(
R12492 T14072 T14070 arg3Of ),(
R12493 T14073 T14067 arg2Of and,from
R12494 T14074 T14073 arg2Of controls,and
R12495 T14074 T14075 arg1Of controls,(
R12496 T14076 T14075 arg2Of n = 10–13,(
R12497 T14077 T14075 arg3Of ),(
R12498 T14079 T14080 arg1Of treated,with
R12499 T14079 T14092 arg1Of treated,and
R12500 T14081 T14082 arg1Of z-YVAD-fmk,(
R12501 T14081 T14086 arg1Of z-YVAD-fmk,or
R12502 T14084 T14082 arg2Of µM,(
R12503 T14084 T14083 arg1Of µM,20
R12504 T14085 T14082 arg3Of ),(
R12505 T14086 T14080 arg2Of or,with
R12506 T14087 T14086 arg2Of Bay11-7082,or
R12507 T14087 T14088 arg1Of Bay11-7082,(
R12508 T14090 T14088 arg2Of µM,(
R12509 T14090 T14089 arg1Of µM,10
R12510 T14091 T14088 arg3Of ),(
R12511 T14092 T14078 arg2Of and,were
R12512 T14092 T14118 arg1Of and,according
R12513 T14092 T14129 arg1Of and,and
R12514 T14093 T14092 arg2Of stimulated,and
R12515 T14093 T14094 arg1Of stimulated,with
R12516 T14096 T14095 arg1Of PAO1,live
R12517 T14096 T14097 arg1Of PAO1,(
R12518 T14096 T14100 arg1Of PAO1,","
R12519 T14098 T14097 arg2Of MOI = 1,(
R12520 T14099 T14097 arg3Of ),(
R12521 T14100 T14107 arg1Of ",",or
R12522 T14101 T14100 arg2Of ATP,","
R12523 T14101 T14102 arg1Of ATP,(
R12524 T14104 T14102 arg2Of mM,(
R12525 T14104 T14103 arg1Of mM,5
R12526 T14105 T14102 arg3Of ),(
R12527 T14107 T14094 arg2Of or,with
R12528 T14107 T14106 arg1Of or,","
R12529 T14108 T14107 arg2Of Poly,or
R12530 T14108 T14109 arg1Of Poly,(
R12531 T14108 T14114 arg1Of Poly,(
R12532 T14110 T14109 arg2Of dA,(
R12533 T14110 T14111 arg1Of dA,:
R12534 T14110 T14112 arg1Of dA,dT
R12535 T14113 T14109 arg3Of ),(
R12536 T14116 T14114 arg2Of µg/ml,(
R12537 T14116 T14115 arg1Of µg/ml,1
R12538 T14117 T14114 arg3Of ),(
R12539 T14119 T14118 arg2Of to,according
R12540 T14121 T14119 arg2Of schedule,to
R12541 T14121 T14120 arg1Of schedule,the
R12542 T14121 T14122 arg1Of schedule,in
R12543 T14123 T14124 arg1Of Figure,1.
R12544 T14123 T14125 arg1Of Figure,(
R12545 T14126 T14125 arg2Of G,(
R12546 T14127 T14125 arg3Of ),(
R12547 T14128 T14122 arg2Of IL-1β,in
R12548 T14128 T14123 arg1Of IL-1β,Figure
R12549 T14131 T14130 arg2Of H,(
R12550 T14132 T14130 arg3Of ),(
R12551 T14134 T14130 arg1Of levels,(
R12552 T14134 T14133 arg1Of levels,IL-8
R12553 T14134 T14135 arg1Of levels,were
R12554 T14134 T14136 arg2Of levels,measured
R12555 T14136 T14129 arg2Of measured,and
R12556 T14136 T14135 arg2Of measured,were
R12557 T14136 T14137 arg1Of measured,at
R12558 T14139 T14137 arg2Of hours,at
R12559 T14139 T14138 arg1Of hours,24
R12560 T14141 T14140 arg1Of analysis,Statistical
R12561 T14141 T14142 arg1Of analysis,was
R12562 T14141 T14143 arg2Of analysis,performed
R12563 T14143 T14142 arg2Of performed,was
R12564 T14143 T14158 arg1Of performed,and
R12565 T14144 T14143 arg3Of using,performed
R12566 T14147 T14144 arg2Of ANOVA,using
R12567 T14147 T14145 arg1Of ANOVA,two
R12568 T14147 T14146 arg1Of ANOVA,way
R12569 T14147 T14148 arg1Of ANOVA,with
R12570 T14147 T14151 arg1Of ANOVA,for
R12571 T14147 T14154 arg1Of ANOVA,*
R12572 T14147 T14156 arg1Of ANOVA,**
R12573 T14150 T14148 arg2Of correction,with
R12574 T14150 T14149 arg1Of correction,Bonferroni
R12575 T14153 T14151 arg2Of comparisons.,for
R12576 T14153 T14152 arg1Of comparisons.,multiple
R12577 T14154 T14155 arg1Of *,","
R12578 T14156 T14155 arg2Of **,","
R12579 T14158 T14157 arg1Of and,","
R12580 T14158 T14163 arg1Of and,0.05
R12581 T14158 T14165 arg1Of and,0.01
R12582 T14158 T14168 arg1Of and,0.001
R12583 T14159 T14160 arg1Of ***,signify
R12584 T14160 T14158 arg2Of signify,and
R12585 T14161 T14160 arg2Of P,signify
R12586 T14161 T14162 arg1Of P,<
R12587 T14163 T14164 arg1Of 0.05,","
R12588 T14164 T14167 arg1Of ",",and
R12589 T14165 T14164 arg2Of 0.01,","
R12590 T14167 T14166 arg1Of and,","
R12591 T14168 T14167 arg2Of 0.001,and
R12879 T14505 T14506 arg1Of Bay11-7082,inhibits
R12880 T14506 T14509 arg1Of inhibits,in
R12881 T14508 T14506 arg2Of production,inhibits
R12882 T14508 T14507 arg1Of production,pro-IL-1β
R12883 T14510 T14509 arg2Of response,in
R12884 T14510 T14511 arg1Of response,to
R12885 T14513 T14511 arg2Of aeruginosa,to
R12886 T14513 T14512 arg1Of aeruginosa,P.
R12887 T14516 T14514 arg1Of cells,PMA-differentiated
R12888 T14516 T14515 arg1Of cells,THP-1
R12889 T14516 T14517 arg1Of cells,were
R12890 T14516 T14518 arg2Of cells,treated
R12891 T14516 T14528 arg2Of cells,harvested
R12892 T14518 T14519 arg1Of treated,with
R12893 T14518 T14527 arg1Of treated,and
R12894 T14520 T14521 arg1Of 10,µM
R12895 T14522 T14520 arg1Of CFTRinh172,10
R12896 T14522 T14523 arg1Of CFTRinh172,or
R12897 T14523 T14519 arg2Of or,with
R12898 T14524 T14525 arg1Of 20,µM
R12899 T14526 T14523 arg2Of Bay11-7082,or
R12900 T14526 T14524 arg1Of Bay11-7082,20
R12901 T14527 T14517 arg2Of and,were
R12902 T14528 T14527 arg2Of harvested,and
R12903 T14528 T14529 arg1Of harvested,after
R12904 T14531 T14530 arg2Of A,(
R12905 T14532 T14530 arg3Of ),(
R12906 T14534 T14530 arg1Of hours,(
R12907 T14534 T14533 arg1Of hours,4
R12908 T14534 T14535 arg1Of hours,(
R12909 T14534 T14538 arg1Of hours,or
R12910 T14536 T14535 arg2Of n = 3,(
R12911 T14537 T14535 arg3Of ),(
R12912 T14538 T14529 arg2Of or,after
R12913 T14540 T14539 arg2Of B,(
R12914 T14541 T14539 arg3Of ),(
R12915 T14542 T14543 arg1Of 0.5,","
R12916 T14543 T14539 arg1Of ",",(
R12917 T14543 T14546 arg1Of ",",and
R12918 T14544 T14543 arg2Of 1,","
R12919 T14546 T14538 arg2Of and,or
R12920 T14546 T14545 arg1Of and,","
R12921 T14548 T14547 arg1Of hours,1.5
R12922 T14548 T14549 arg1Of hours,(
R12923 T14550 T14549 arg2Of n = 3,(
R12924 T14551 T14549 arg3Of ),(
R12925 T14552 T14546 arg2Of stimulation,and
R12926 T14552 T14548 arg1Of stimulation,hours
R12927 T14552 T14553 arg1Of stimulation,with
R12928 T14554 T14553 arg2Of PAO1,with
R12929 T14557 T14555 arg1Of blot,One
R12930 T14557 T14556 arg1Of blot,representative
R12931 T14557 T14558 arg1Of blot,is
R12932 T14557 T14559 arg2Of blot,shown
R12933 T14559 T14558 arg2Of shown,is
R12934 T14559 T14560 arg1Of shown,with
R12935 T14562 T14560 arg2Of graph,with
R12936 T14562 T14561 arg1Of graph,a
R12937 T14562 T14563 arg1Of graph,of
R12938 T14562 T14569 arg1Of graph,over
R12939 T14568 T14563 arg2Of values,of
R12940 T14568 T14564 arg1Of values,the
R12941 T14568 T14565 arg1Of values,averaged
R12942 T14568 T14566 arg1Of values,fluorescence
R12943 T14568 T14567 arg1Of values,intensity
R12944 T14571 T14569 arg2Of experiments,over
R12945 T14571 T14570 arg1Of experiments,3
R13030 T14674 T14675 arg1Of Disruption,of
R13031 T14674 T14678 arg1Of Disruption,does
R13032 T14674 T14680 arg1Of Disruption,increase
R13033 T14677 T14675 arg2Of activity,of
R13034 T14677 T14676 arg1Of activity,CFTR
R13035 T14680 T14678 arg2Of increase,does
R13036 T14680 T14679 arg1Of increase,not
R13037 T14682 T14680 arg2Of production,increase
R13038 T14682 T14681 arg1Of production,IL-1β
R13039 T14682 T14683 arg1Of production,in
R13040 T14684 T14685 arg1Of PBMCs,or
R13041 T14685 T14683 arg2Of or,in
R13042 T14686 T14685 arg2Of macrophages,or
R13043 T14687 T14688 arg1Of PBMCs,from
R13044 T14687 T14699 arg1Of PBMCs,were
R13045 T14687 T14700 arg2Of PBMCs,treated
R13046 T14687 T14743 arg1Of PBMCs,rols
R13047 T14687 T14744 arg2Of PBMCs,(n = 3) were d
R13048 T14690 T14689 arg1Of patients,CF
R13049 T14690 T14691 arg1Of patients,(
R13050 T14690 T14694 arg1Of patients,and
R13051 T14692 T14691 arg2Of n = 15,(
R13052 T14693 T14691 arg3Of ),(
R13053 T14694 T14688 arg2Of and,from
R13054 T14695 T14694 arg2Of controls,and
R13055 T14695 T14696 arg1Of controls,(
R13056 T14697 T14696 arg2Of n = 13,(
R13057 T14698 T14696 arg3Of ),(
R13058 T14700 T14699 arg2Of treated,were
R13059 T14700 T14701 arg1Of treated,with
R13060 T14700 T14723 arg1Of treated,1β
R13061 T14702 T14701 arg2Of CFTRinh172,with
R13062 T14702 T14703 arg1Of CFTRinh172,(
R13063 T14702 T14707 arg1Of CFTRinh172,for
R13064 T14702 T14711 arg1Of CFTRinh172,to
R13065 T14705 T14703 arg2Of µM,(
R13066 T14705 T14704 arg1Of µM,10
R13067 T14706 T14703 arg3Of ),(
R13068 T14709 T14707 arg2Of hours,for
R13069 T14709 T14708 arg1Of hours,18
R13070 T14711 T14710 arg1Of to,prior
R13071 T14712 T14711 arg2Of stimulation,to
R13072 T14712 T14713 arg1Of stimulation,with
R13073 T14715 T14714 arg1Of PAO1,live
R13074 T14715 T14716 arg1Of PAO1,(
R13075 T14715 T14719 arg1Of PAO1,.
R13076 T14717 T14716 arg2Of MOI = 1,(
R13077 T14718 T14716 arg3Of ),(
R13078 T14720 T14719 arg2Of (,.
R13079 T14721 T14719 arg3Of A,.
R13080 T14722 T14713 arg2Of ) IL-,with
R13081 T14722 T14715 arg1Of ) IL-,PAO1
R13082 T14725 T14724 arg2Of n,a
R13083 T14726 T14724 arg3Of d,a
R13084 T14728 T14724 arg1Of IL-8 produ,a
R13085 T14728 T14727 arg1Of IL-8 produ,(B)
R13086 T14728 T14729 arg1Of IL-8 produ,cti
R13087 T14728 T14730 arg2Of IL-8 produ,on was m
R13088 T14730 T14729 arg2Of on was m,cti
R13089 T14730 T14731 arg1Of on was m,ea
R13090 T14735 T14734 arg2Of 4,2
R13091 T14736 T14734 arg3Of h,2
R13092 T14737 T14731 arg2Of ours. (C),ea
R13093 T14737 T14732 arg1Of ours. (C),su
R13094 T14737 T14733 arg1Of ours. (C),red at
R13095 T14737 T14734 arg1Of ours. (C),2
R13096 T14737 T14738 arg1Of ours. (C),Mono
R13097 T14739 T14738 arg2Of cytes fr,Mono
R13098 T14739 T14740 arg1Of cytes fr,o
R13099 T14741 T14740 arg2Of m con,o
R13100 T14742 T14740 arg3Of t,o
R13101 T14744 T14723 arg2Of (n = 3) were d,1β
R13102 T14744 T14729 modOf (n = 3) were d,cti
R13103 T14744 T14743 arg2Of (n = 3) were d,rols
R13104 T14744 T14745 arg1Of (n = 3) were d,iffe
R13105 T14746 T14745 arg2Of rentiated i,iffe
R13106 T14747 T14748 arg1Of Macrophages,were
R13107 T14747 T14749 arg2Of Macrophages,treated
R13108 T14749 T14748 arg2Of treated,were
R13109 T14749 T14750 arg1Of treated,with
R13110 T14751 T14750 arg2Of CFTRinh172,with
R13111 T14751 T14752 arg1Of CFTRinh172,","
R13112 T14751 T14753 arg2Of CFTRinh172,stimulated
R13113 T14751 T14759 arg2Of CFTRinh172,measured
R13114 T14753 T14755 arg1Of stimulated,per
R13115 T14753 T14758 arg1Of stimulated,and
R13116 T14755 T14754 arg1Of per,as
R13117 T14756 T14755 arg2Of monocytes,per
R13118 T14758 T14757 arg1Of and,","
R13119 T14759 T14758 arg2Of measured,and
R13120 T14759 T14760 arg1Of measured,for
R13121 T14759 T14763 arg1Of measured,at
R13122 T14762 T14760 arg2Of production,for
R13123 T14762 T14761 arg1Of production,IL-1β
R13124 T14765 T14763 arg2Of hours,at
R13125 T14765 T14764 arg1Of hours,24
R13126 T14768 T14766 arg1Of cells,THP-1
R13127 T14768 T14767 arg1Of cells,reporter
R13128 T14768 T14769 arg1Of cells,were
R13129 T14768 T14770 arg2Of cells,treated
R13130 T14768 T14781 arg2Of cells,measured
R13131 T14770 T14771 arg1Of treated,with
R13132 T14770 T14775 arg1Of treated,prior
R13133 T14770 T14780 arg1Of treated,and
R13134 T14772 T14771 arg2Of CFTRinh172,with
R13135 T14774 T14773 arg1Of hours,24
R13136 T14775 T14774 arg1Of prior,hours
R13137 T14775 T14776 arg1Of prior,to
R13138 T14777 T14776 arg2Of stimulation,to
R13139 T14777 T14778 arg1Of stimulation,with
R13140 T14779 T14778 arg2Of PAO1,with
R13141 T14780 T14769 arg2Of and,were
R13142 T14781 T14780 arg2Of measured,and
R13143 T14781 T14782 arg1Of measured,for
R13144 T14784 T14783 arg2Of D,(
R13145 T14785 T14783 arg3Of ),(
R13146 T14787 T14783 arg1Of secretion,(
R13147 T14787 T14786 arg1Of secretion,IL-1β
R13148 T14787 T14788 arg1Of secretion,","
R13149 T14788 T14794 arg1Of ",",and
R13150 T14790 T14789 arg2Of E,(
R13151 T14791 T14789 arg3Of ),(
R13152 T14792 T14788 arg2Of IL-8,","
R13153 T14792 T14789 arg1Of IL-8,(
R13154 T14794 T14782 arg2Of and,for
R13155 T14794 T14793 arg1Of and,","
R13156 T14796 T14795 arg2Of F,(
R13157 T14797 T14795 arg3Of ),(
R13158 T14799 T14794 arg2Of activity,and
R13159 T14799 T14795 arg1Of activity,(
R13160 T14799 T14798 arg1Of activity,NF-κB/AP-1
R13161 T14799 T14800 arg1Of activity,at
R13162 T14802 T14800 arg2Of hours,at
R13163 T14802 T14801 arg1Of hours,24
R13164 T14802 T14803 arg1Of hours,(
R13165 T14804 T14803 arg2Of n = 4,(
R13166 T14805 T14803 arg3Of ),(
R13337 T15015 T15014 arg1Of Activators,Inflammasome

sentences

Id Subject Object Predicate Lexical cue
T72 0-69 Sentence denotes Inflammasome-Mediated IL-1β Production in Humans with Cystic Fibrosis
T73 117-127 Sentence denotes Background
T74 128-287 Sentence denotes Inflammation and infection are major determinants of disease severity and consequently, the quality of life and outcome for patients with cystic fibrosis (CF).
T75 288-346 Sentence denotes Interleukin-1 beta (IL-1β) is a key inflammatory mediator.
T76 347-428 Sentence denotes Secretion of biologically active IL-1β involves inflammasome-mediated processing.
T77 429-526 Sentence denotes Little is known about the contribution of IL-1β and the inflammasomes in CF inflammatory disease.
T78 527-651 Sentence denotes This study examines inflammasome-mediated IL-1β production in CF bronchial epithelial cell lines and human patients with CF.
T79 653-660 Sentence denotes Results
T80 661-886 Sentence denotes Bronchial epithelial cell lines were found to produce negligible amounts of basal or stimulated IL-1β compared to hematopoeitic cells and they did not significantly upregulate caspase-1 activity upon inflammasome stimulation.
T81 887-1091 Sentence denotes In contrast, peripheral blood mononuclear cells (PBMCs) from both CF and healthy control subjects produced large amounts of IL-1β and strongly upregulated caspase-1 activity upon inflammasome stimulation.
T82 1092-1243 Sentence denotes PBMCs from CF patients and controls displayed similar levels of caspase-1 activation and IL-1β production when stimulated with inflammasome activators.
T83 1244-1340 Sentence denotes This IL-1β production was dependent on NF-κB activity and could be enhanced by priming with LPS.
T84 1341-1502 Sentence denotes Finally, chemical inhibition of CFTR activity in control PBMCs and THP-1 cells did not significantly alter IL-1β or IL-8 production in response to P. aeruginosa.
T85 1504-1514 Sentence denotes Conclusion
T86 1515-1624 Sentence denotes Hematopoeitic cells appear to be the predominant source of inflammasome-induced pro-inflammatory IL-1β in CF.
T87 1625-1778 Sentence denotes PBMCs derived from CF subjects display preserved inflammasome activation and IL-1β secretion in response to the major CF pathogen Pseudomonas aeruginosa.
T88 1779-1954 Sentence denotes However, our data do not support the hypothesis that increased IL-1β production in CF subjects is due to an intrinsic increase in NF-κB activity through loss of CFTR function.
T930 1970-2149 Sentence denotes Cystic fibrosis is an autosomal recessive disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as a chloride ion channel.
T931 2150-2331 Sentence denotes CF remains one of the most common lethal genetic diseases in populations of European descent with the current average lifespan of CF patients approximately 40 years of age [1], [2].
T932 2332-2455 Sentence denotes Recurrent inflammatory pulmonary exacerbation is the primary cause of lung disease progression and ultimately, death in CF.
T933 2456-2615 Sentence denotes Controlled inflammation is important for fighting infection, but in excess, it becomes destructive to host cells and to the architecture of the lungs [2], [3].
T934 2616-2748 Sentence denotes In CF, airway epithelial cells have been shown to produce an exaggerated pro-inflammatory cytokine response to stimulation [4], [5].
T935 2749-2914 Sentence denotes It is unclear whether this heightened inflammatory response is intrinsic to cells lacking CFTR or whether it is a result of chronic polymicrobial infection [6], [7].
T936 2915-3137 Sentence denotes Regardless of this controversy, identifying and targeting relevant inflammatory mediators is a critical step in developing more specific therapeutic approaches to control inflammation and improve health outcomes in CF [8].
T937 3138-3198 Sentence denotes Interleukin-1 beta (IL-1β) is a major inflammatory mediator.
T938 3199-3514 Sentence denotes Its physiological effects are diverse and potentially important to the pathogenesis of lung exacerbations in CF, including the generation of fever, the recruitment of inflammatory effector cells, the induction of other pro-inflammatory cytokines such as IL-6 and IL-8, and the shaping of T cell responses [9], [10].
T939 3515-3650 Sentence denotes Following initiation of the NF-κB signaling cascade, IL-1β is produced in the cytosol as a biologically inactive full-length pro-IL-1β.
T940 3651-3978 Sentence denotes Pro-IL-1β is subsequently converted into its active form by cytosolic protein complexes termed “inflammasomes.” Inflammasomes assemble in response to certain cellular danger signals and mediate the auto-activation of caspase-1 [9], [11], which cleaves pro-IL-1β and pro-IL-18 into their biologically active forms for secretion.
T941 3979-4028 Sentence denotes Four distinct inflammasomes have been recognized.
T942 4029-4211 Sentence denotes These are the NLRP1 [12], NLRP3 [13], [14], NLRC4 [15], [16], and AIM2 inflammasomes [17], [18], which respond to a variety of different microbial signatures and danger signals [11].
T943 4212-4347 Sentence denotes P. aeruginosa, one of the most common and clinically relevant pathogens among CF patients, activates the NLRC4 inflammasome [19], [20].
T944 4348-4499 Sentence denotes Infection with P. aeruginosa triggers an increase in levels of IL-1β, IL-6, and IL-8 in bronchoalveolar lavage fluid (BALF) from patients with CF [21].
T945 4500-4687 Sentence denotes Inflammasome responses depend on NF-κB signaling, where NF-κB is important in both the upregulation of specific inflammasome components [22], [23], as well as IL-1β expression [24], [25].
T946 4688-4782 Sentence denotes Previous studies support a role for IL-1β in the pathogenesis of CF inflammatory lung disease.
T947 4783-4968 Sentence denotes Levels of IL-1β are increased in BALF from CF patients with infection [21], [26], [27], [28] and this increase has been temporally associated with a clinical response to treatment [21].
T948 4969-5087 Sentence denotes Polymorphisms in the IL1B gene have also been associated with varying degrees of disease severity in CF patients [29].
T949 5088-5355 Sentence denotes Murine models of CFTR dysfunction have exhibited significant increases in IL-1β expression or secretion in macrophages [30], [31], and support the hypothesis that the loss of CFTR increases NF-κB activation under basal and stimulatory conditions [4], [5], [32], [33].
T950 5356-5619 Sentence denotes Finally, replacement of chloride ions with glutamate or gluconate in cell culture media increases secretion of IL-1β in response to NLRP3 stimulation by adenosine triphosphate (ATP) [34], implying an inhibitory role for extracellular chloride in NLRP3 activation.
T951 5620-5746 Sentence denotes Taken together, these data implicate the involvement of IL-1β and consequently, the inflammasomes, in CF inflammatory disease.
T2604 5757-5864 Sentence denotes Airway epithelial cells do not produce significant amounts of IL-1β in response to inflammasome stimulation
T2605 5865-5960 Sentence denotes The inflammasomes and their respective activators examined in this study are listed in Table 1.
T2606 5961-6027 Sentence denotes Cells were stimulated in accordance with the schedule in Figure 1.
T2607 6028-6188 Sentence denotes In CF, airway epithelial cells have been shown to possess a hyper-inflammatory phenotype and produce an exaggerated pro-inflammatory cytokine response [4], [5].
T2608 6189-6446 Sentence denotes To determine if airway epithelial cells contribute to the increased IL-1β production in patients with CF, CF and control bronchial epithelial cell lines were stimulated with the inflammasome inducers P. aeruginosa strain PAO1 (PAO1) and LPS followed by ATP.
T2609 6447-6698 Sentence denotes IL-1β levels in cell culture supernatants were not greatly increased in either the CF or control cell lines (Fig. 2a–d), although a small increase in IL-1β production was detected in NuLi-1 and CuFi-1 cells, but not in S9 and IB3-1 cells, by 24 hours.
T2610 6699-6867 Sentence denotes In contrast, these airway cells were highly responsive to other inflammatory stimuli, such as recombinant IL-1β, producing large quantities of IL-8 (Fig. 2a–d inserts).
T3090 7936-8050 Sentence denotes Airway epithelial cells do not significantly upregulate caspase-1 activity in response to inflammasome stimulation
T3091 8051-8171 Sentence denotes To examine if inflammasome activation occurs in these airway cells, caspase-1 activity was quantified by flow cytometry.
T3092 8172-8283 Sentence denotes There was no significant increase upon stimulation with live PAO1 or LPS+ATP at the times examined (Fig. 3a–b).
T3093 8284-8543 Sentence denotes Because previous studies have indicated a role for caspase-1 in the activation of NF-κB through Toll-like receptor (TLR) signaling [35], we examined whether chemical inhibition of caspase-1 altered NF-κB-dependent IL-6 production in response to P. aeruginosa.
T3094 8544-8679 Sentence denotes However, treatment with the caspase-1 inhibitor z-YVAD-fmk (YVAD) did not decrease IL-6 secretion by airway epithelial cells (Fig. 3c).
T3401 9425-9553 Sentence denotes CD14 positive monocytes from CF patients and controls show similar increases in caspase-1 activity upon inflammasome stimulation
T3402 9554-9635 Sentence denotes Monocytes were identified in PBMC populations using CD14 as a phenotyping marker.
T3403 9636-9899 Sentence denotes CD14 positive monocytes from CF patients and healthy controls showed a significant increase in caspase-1 activation upon stimulation with LPS+ATP, PAO1, and LPS+Poly(dA:dT) (Fig. 4a) but this activation was not different between CF and control subjects (Fig. 4b).
T3629 10426-10520 Sentence denotes PBMCs from CF patients do not produce increased amounts of IL-1β upon inflammasome stimulation
T3630 10521-10674 Sentence denotes Previous studies have shown that the loss of CFTR results in increased NF-κB activity and pro-inflammatory cytokine secretion [4], [5], [32], [36], [37].
T3631 10675-10887 Sentence denotes To further examine this relationship, PBMCs from CF patients and healthy adult controls were stimulated with PAO1, LPS+ATP, and LPS+Poly(dA:dT), to activate the NLRC4, NLRP3, and AIM2 inflammasomes, respectively.
T3632 10888-11030 Sentence denotes By 24 hours of stimulation, CF PBMCs did not produce increased amounts of IL-1β (Fig. 5a) or IL-8 (Fig. 5b) when compared to healthy controls.
T3633 11031-11180 Sentence denotes However, we did notice a transient decrease (P<0.001) in the amount of IL-1β produced by CF cells in response to LPS+ATP at 6 hours (data not shown).
T3634 11181-11379 Sentence denotes Stimulation of PBMCs with P. aeruginosa that lacks exsA (PAO1ΔexsA), a key regulator of type III secretion, produced three-fold less IL-1β compared to the parental PAO1 strain by 24 hours (Fig. 5a).
T3635 11380-11490 Sentence denotes Inflammasome stimulation without priming did not result in any IL-1β production in either CF or control PBMCs.
T3636 11491-11718 Sentence denotes Contrary to our hypothesis, these results indicate that PBMCs from CF patients do not display increased production of IL-1β or IL-8 with inflammasome activation nor do they suggest any increased basal or induced NF-κB activity.
T3637 11719-11848 Sentence denotes These results are consistent with our observation that caspase-1 activity is not different between CF and control PBMCs (Fig. 4).
T4383 12380-12454 Sentence denotes NF-κB activation is required for IL-1β and IL-8 responses to P. aeruginosa
T4384 12456-12629 Sentence denotes We confirmed the dependence of PAO1-induced IL-1β and IL-8 production on NF-κB activation using THP-1 cells expressing a reporter driven by NF-κB and AP-1 response elements.
T4385 12630-12878 Sentence denotes We found that stimulation of primed THP-1 reporter cells with heat-killed PAO1 produced the highest levels of NF-κB/AP-1 activation (Fig. 6a) and this correlated with IL-8 secretion (Fig. 6b) but negligible amounts of IL-1β were secreted (Fig. 6c).
T4386 12879-13045 Sentence denotes Stimulation of primed THP-1 reporter cells with live PAO1 did not significantly increase NF-κB/AP-1 activity (Fig. 6a) or IL-8 secretion (Fig. 6b) over priming alone.
T4387 13046-13199 Sentence denotes However, IL-1β production was greatly augmented over primed cells stimulated with heat-killed PAO1 or unprimed cells stimulated with live PAO1 (Fig. 6c).
T4388 13200-13407 Sentence denotes This confirmed that NF-κB activation alone is not sufficient for maximal IL-1β secretion, but increased priming of NF-κB is capable of augmenting IL-1β production and secretion upon inflammasome stimulation.
T4389 13408-13757 Sentence denotes Dependency of these responses on NF-κB was confirmed by pharmacologic inhibition of NF-κB using the Bay11-7082 inhibitor of IκBα phosphorylation, which significantly reduced NF-κB/AP-1 activation (P<0.001) (Fig. 6d) and the subsequent production of IL-8 (P<0.01) (Fig. 6e) and IL-1β (P<0.001) (Fig. 6f) in response to both heat-killed and live PAO1.
T4390 13758-13868 Sentence denotes These results were also verified in CF and control PBMCs for each inflammasome examined (P<0.001) (Fig. 6g–h).
T4391 13869-14046 Sentence denotes Overall these results confirm that NF-κB is an important modulator of IL-1β production and that increased activation of NF-κB augments inflammasome-mediated production of IL-1β.
T5207 15230-15299 Sentence denotes Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa
T5208 15301-15411 Sentence denotes In addition to inhibition of NF-κB activity, Bay11-7082 can also directly inhibit the NLRP3 inflammasome [38].
T5209 15412-15623 Sentence denotes To validate its use in this study as an NF-κB inhibitor, western blots for pro-IL-1β were performed alongside inhibition of CFTR activity by CFTRinh172 in response to PAO1 at 4 hours after stimulation (Fig. 7a).
T5210 15624-15740 Sentence denotes Our results indicate that Bay11-7082 prevents production of pro-IL-1β whereas CFTRinh172 does not seem to affect it.
T5211 15741-15882 Sentence denotes This was further corroborated by the ability Bay11-7082 to inhibit IκBα degradation at 0.5, 1, and 1.5 hours post PAO1 stimulation (Fig. 7b).
T5497 16297-16384 Sentence denotes Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells
T5498 16385-16507 Sentence denotes A previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34].
T5499 16508-16725 Sentence denotes To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa.
T5500 16726-16838 Sentence denotes Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b).
T5501 16839-16977 Sentence denotes Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d).
T5502 16978-17085 Sentence denotes IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.
T6037 17820-18006 Sentence denotes Levels of IL-1β are increased in the BALF of CF patients but the cellular source of this cytokine and its production in the context of targeted inflammasome activation are still unclear.
T6038 18007-18176 Sentence denotes We first studied airway epithelial cells due to their role in barrier function, proximity to infection, and ability to produce high levels of pro-inflammatory cytokines.
T6039 18177-18416 Sentence denotes However, we found that bronchial epithelial cells do not produce significant amounts of IL-1β and do not show a significant increase in caspase-1 activation in response to PAO1 and LPS+ATP, in comparison to hematopoeitic mononuclear cells.
T6040 18417-18526 Sentence denotes Hematopoeitically-derived cells, such as monocytes and macrophages, appear to be a principal source of IL-1β.
T6041 18527-18726 Sentence denotes CFTR is expressed in alveolar macrophages [32], [39] as well as in PBMCs at both the mRNA and protein level [40], [41], and its loss is frequently associated with an augmented inflammatory phenotype.
T6042 18727-19110 Sentence denotes Despite our findings indicating that bronchial epithelial cells when grown in vitro are unlikely to be significantly involved in the direct production of IL-1β (Fig. 2a–d), others have shown their capacity to respond to alveolar macrophage-derived IL-1β and to amplify the inflammatory response through the induction of chemokines and recruitment of inflammatory effector cells [42].
T6043 19111-19338 Sentence denotes This interaction may constitute a critical component to effective host defense and diminishing the capacity of host cells to respond to IL-1β may leave the host susceptible to infections by pathogens such as P. aeruginosa [43].
T6044 19339-19485 Sentence denotes Conversely, overproduction of IL-1β can also play a key role in chronic inflammatory responses and cause damage to the lung parenchyma [44], [45].
T6045 19486-19694 Sentence denotes Although we hypothesized that CF cells would secrete increased amounts of IL-1β, we found that IL-1β production in CF PBMCs was not increased upon inflammasome stimulation as compared to controls (Fig. 5a–b).
T6046 19695-19924 Sentence denotes This was in contrast to a previous study from our group, which showed increased IL-1β production by CF PBMCs in response to LPS [46], although this difference may be accounted for by technical issues in stimulation time and dose.
T6047 19925-20089 Sentence denotes Moreover, IL-1β production was not increased in CF PBMCs with inflammasome stimulation alone as would be anticipated if there were basal levels of NF-κB activation.
T6048 20090-20319 Sentence denotes Cells deficient in CFTR are thought to exhibit an increased basal level of NF-κB activity, which leads to increased pro-inflammatory cytokine production including an increased availability of pro-IL-1β for cleavage and secretion.
T6049 20320-20488 Sentence denotes This amplification of IL-1β secretion was shown by priming THP-1 monocytes and PBMCs with heat-killed P. aeruginosa or LPS prior to stimulation with live P. aeruginosa.
T6050 20489-20600 Sentence denotes This dramatically increased IL-1β secretion over stimulation with live P. aeruginosa without priming (Fig. 6c).
T6051 20601-20742 Sentence denotes Similarly, if CF PBMCs expressed increased basal NF-κB activity, there would be an increase in IL-1β secretion in the absence of LPS priming.
T6052 20743-20819 Sentence denotes However, no increase in IL-1β was observed under basal or primed conditions.
T6053 20820-20898 Sentence denotes Studies investigating the production of IL-1β have been somewhat inconsistent.
T6054 20899-21160 Sentence denotes A study by Reininger et al. [43] provided evidence that human bronchial epithelial cells possessing the ΔF508 CFTR mutation had a slightly reduced capacity to produce IL-1β and lacked the ability to induce an early NF-κB activation in response to P. aeruginosa.
T6055 21161-21411 Sentence denotes Conversely, a study by Kotrange et al. [31] found that murine bone marrow-derived macrophages expressing ΔF508-CFTR produced increased amounts of IL-1β when compared to macrophages expressing normal CFTR in response to Burkholderia cenocepacia K56-2.
T6056 21412-21726 Sentence denotes The differentiation of monocytes into macrophages may partly account for the differences observed with the study by Kotrange. Inflammasome-mediated IL-1β production by monocytes and PBMCs does differ from macrophages [47], [48], [49] and macrophages are found to have higher expression of CFTR over monocytes [50].
T6057 21727-21910 Sentence denotes However, as monocytes and other PBMCs express CFTR [50], [51] and produce large amounts of IL-1β, they are adequate models to examine the effects of CFTR function on IL-1β production.
T6058 21911-21977 Sentence denotes Other hematopoeitic cells may also contribute to IL-1β production.
T6059 21978-22160 Sentence denotes For example, neutrophil counts can be significantly increased in the lungs of CF patients [52], [53], [54] and may produce mature IL-1β through caspase-1 independent mechanisms [55].
T6060 22161-22260 Sentence denotes The role of NF-κB activation in inflammasome activation and IL-1β secretion is not straightforward.
T6061 22261-22406 Sentence denotes Studies have revealed an essential role for NF-κB activation in the production of pro-IL-1β and inflammasome components such as NLRP3 [22], [23].
T6062 22407-22599 Sentence denotes In contrast, deletion of IKKβ, a kinase essential in NF-κB activation, increases IL-1β secretion in murine macrophages [56], [57] and demonstrates a dual role for NF-κB in regulation of IL-1β.
T6063 22600-22791 Sentence denotes To address this uncertainty in our experiments we also quantified IL-8, an important CF cytokine and marker of NF-κB activation [58], and found no differences between CF and control subjects.
T6064 22792-22932 Sentence denotes Similarly, levels of intracellular pro-IL-1β in THP-1 cells were dependent on NF-κB activity and did not increase with CFTRinh172 treatment.
T6065 22933-23081 Sentence denotes Subsequent treatment of THP-1 cells and PBMCs with the NF-κB inhibitor Bay11-7082 significantly inhibited both IL-1β and IL-8 secretion (Fig. 6d–h).
T6066 23082-23173 Sentence denotes Therefore, the IL-1β and IL-8 responses observed were both dependent upon NF-κB activation.
T6067 23174-23454 Sentence denotes Priming with heat-killed P. aeruginosa, like LPS, is unable to induce a strong IL-1β response as compared to live P. aeruginosa (Fig. 6c), but generated greater NF-κB/AP-1 activation (Fig. 6a) and IL-8 secretion (Fig. 6b) than live bacteria despite stimulation at equivalent MOIs.
T6068 23455-23594 Sentence denotes This may be indicative of the different degree and quality of the inflammatory response generated by live as opposed to dead bacteria [59].
T6069 23595-23784 Sentence denotes Potential shortcomings of these experiments include its translatability to lung disease and issues related to the hypermutability of P. aeruginosa during the evolution of chronic infection.
T6070 23785-24274 Sentence denotes Although the responses measured in peripheral blood cells may not completely reflect the responses occurring in the CF lung, PBMCs have a number of useful advantages: (i) PBMCs are not subject to alterations that may emerge from long-term cell culture, cloning and immortalization, and (ii) PBMCs express a large repertoire of innate immune receptors and secrete a broad array of cytokines and chemokines allowing comprehensive analysis of the modulation of inflammatory responses by CFTR.
T6071 24275-24412 Sentence denotes Consideration must also be given to the nature of P. aeruginosa infection and genotypic changes in P. aeruginosa as infection progresses.
T6072 24413-24543 Sentence denotes P. aeruginosa mediates inflammasome activation through its type III secretion system (T3SS) and the NLRC4 inflammasome [19], [20].
T6073 24544-24678 Sentence denotes However, clones of P. aeruginosa established during chronic infection may accumulate mutations in virulence factors such as exsA [60].
T6074 24679-24961 Sentence denotes By employing a deletion mutant in exsA, the key regulator in T3SS transcription, we confirmed that the T3SS is important for IL-1β secretion (Fig. 5a–b), and that depending on the adaptation in type III secretion, the host IL-1β response may be up or downregulated [19], [60], [61].
T6075 24962-25179 Sentence denotes In conclusion, our data are consistent with a role for hematopoietic cells, not airway epithelial cells, as the major source of inflammasome-mediated IL-1β production in the lungs in response to ATP and P. aeruginosa.
T6076 25180-25318 Sentence denotes Furthermore, we find little evidence to support an increased IL-1β inflammatory response to NF-κB/Inflammasome stimulation in CF patients.
T6077 25319-25533 Sentence denotes Further studies are warranted to determine if adaptations of P. aeruginosa during the course of chronic lung infection alters inflammasome activation, and whether this can be correlated with disease severity in CF.
T9309 25558-25574 Sentence denotes Ethics Statement
T9310 25575-25770 Sentence denotes Blood samples were obtained with informed written consent from control subjects and CF patients at the BC Children's Hospital. Consent was obtained for children by their parent or legal guardian.
T9311 25771-25854 Sentence denotes Subjects 7 years of age and older were required to provide informed assent as well.
T9312 25855-25929 Sentence denotes Protocols were approved by the Clinical Research Ethics Board (H09-01192).
T9454 25931-25943 Sentence denotes Cell Culture
T9455 25944-26195 Sentence denotes CF (IB3-1 and CuFi-1) and control (S9 and NuLi-1) cells were obtained from the American Type Culture Collection. IB3-1 cells were derived from a patient expressing the ΔF508 and W1282X mutations and CuFi-1 were derived from a ΔF508 homozygous patient.
T9456 26196-26379 Sentence denotes S9 cells are IB3-1 cells that have been transfected with CFTR using an adeno-associated viral vector and NuLi-1 cells were derived from a patient possessing a wild-type CFTR genotype.
T9457 26380-26510 Sentence denotes THP1-XBlue cells stably express a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB and AP-1 (Invivogen).
T9458 26511-26601 Sentence denotes Cells were cultured as recommended by their respective suppliers using standard protocols.
T9459 26602-26760 Sentence denotes S9 and IB3-1 cells were cultured in basal LHC-8 (Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T9460 26761-27023 Sentence denotes NuLi-1/CuFi-1 cells were cultured in BEBM serum-free medium (Lonza) with supplement bullet kit (EGF, hydrocortisone, bovine pituitary extract, transferrin, bovine insulin, triiodothyronine, epinephrine, retinoic acid), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T9461 27024-27183 Sentence denotes PBMCs from CF patients and controls were cultured in RPMI-1640 (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, and 1 mM sodium pyruvate (complete RPMI).
T9462 27184-27332 Sentence denotes THP1-XBlue cells were cultured in complete RPMI with the addition of zeocin (100 µg/ml) to select for cells expressing the SEAP NF-κB/AP-1 reporter.
T9463 27333-27516 Sentence denotes Prior to stimulation, bronchial epithelial cell lines were plated in coated [62] 96-well plates (BD Biosciences) at 3×104 cells/well unless indicated, and allowed to adhere overnight.
T9464 27517-27694 Sentence denotes Plates for S9 and IB3-1 stimulations were coated in a mixture of bovine serum albumin (100 µg/ml), fibronectin (10 µg/ml), and bovine collage type I (30 µg/ml) (BD Biosciences).
T9465 27695-27785 Sentence denotes Plates for NuLi-1 and CuFi-1 were coated with collagen type IV (60 µg/ml) (Sigma Aldrich).
T9466 27786-27886 Sentence denotes PBMCs were plated in 96-well plates at a density of 1.5×105 cells/well in 200 µl (7.5×105 cells/mL).
T9467 27887-28106 Sentence denotes THP-1 reporter cells were differentiated into a macrophage-like phenotype using 50 ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich) for 24 hours at a density of 1×105 cells/well in 200 µl (5×105 cells/ml).
T9468 28107-28194 Sentence denotes Cells were washed with PBS and allowed to rest a further 42 hours prior to stimulation.
T10414 28196-28224 Sentence denotes CF and control subject PBMCs
T10415 28225-28384 Sentence denotes The diagnosis of CF was established by typical clinical features, increased sweat chloride concentrations (>60 mmol/l), and detection of CF-inducing mutations.
T10416 28385-28576 Sentence denotes All patients with CF were clinically stable at the time of blood donation, and we excluded any subjects who were receiving systemic corticosteroids due to potential immunomodulatory activity.
T10417 28577-28635 Sentence denotes Control samples were provided by healthy adult volunteers.
T10418 28636-28846 Sentence denotes In previously published work we have demonstrated that TLR-mediated inflammatory responses are stable in humans from birth to 60 years old [63], therefore did not age-match the CF patients and control subjects.
T10419 28847-29019 Sentence denotes Peripheral blood was collected in sodium heparin tubes (BD Biosciences) and PBMCs were isolated using density gradient centrifugation on Ficoll-Paque™ Plus (GE Healthcare).
T10420 29020-29114 Sentence denotes The layer containing PBMCs was isolated, washed twice in PBS and resuspended in complete RPMI.
T10421 29115-29217 Sentence denotes Cells were enumerated by trypan blue exclusion using the Countess automated cell counter (Invitrogen).
T10422 29218-29377 Sentence denotes For derivation of macrophages from monocytes, monocytes were allowed to adhere to plastic for 2 hours in RPMI 1640 after which non-adherent cells were removed.
T10423 29378-29480 Sentence denotes Monocytes were allowed to differentiate in RPMI 1640 supplemented with 10% human AB serum for 10 days.
T10861 29482-29526 Sentence denotes Cell stimulation and cytokine quantification
T10862 29527-29619 Sentence denotes Bronchial epithelial cells were plated and allowed to adhere overnight prior to stimulation.
T10863 29620-29765 Sentence denotes Bronchial epithelial cells were rested or primed with LPS for 5 hours and stimulated with live P. aeruginosa PAO1 or ATP for the times indicated.
T10864 29766-29888 Sentence denotes PBMCs and THP-1 reporter cells were either rested or primed with LPS (Invivogen) or heat-killed PAO1 overnight (16 hours).
T10865 29889-30040 Sentence denotes The next day the cells were challenged with live PAO1, PAO1ΔexsA, ATP (Invivogen), or Poly(dA:dT) (Sigma Aldrich) for the times indicated (see Fig. 1).
T10866 30041-30206 Sentence denotes For stimulations with Poly(dA:dT), lipofectamine LTX was used at a 1∶1 (w∶v) ratio of µg of DNA to µl of lipofectamine and was mixed 30 minutes prior to stimulation.
T10867 30207-30296 Sentence denotes The NF-κB inhibitor Bay11-7082 (Invivogen) was added to cultures 1 hour prior to priming.
T10868 30297-30386 Sentence denotes If no priming was involved, inhibitor was added 1 hour prior to inflammasome stimulation.
T10869 30387-30498 Sentence denotes The CFTR inhibitor CFTRinh172 (Sigma Aldrich) was added to cultures 18 hours prior to inflammasome stimulation.
T10870 30499-30609 Sentence denotes The caspase-1 inhibitor z-YVAD-fmk (Biovision) was added to cultures 1 hour prior to inflammasome stimulation.
T10871 30610-30798 Sentence denotes Supernatants were collected and stored at −20°C. Cytokines released into supernatants from PBMCs stimulated with inflammasome activators were quantified using sandwich ELISA (eBioscience).
T11380 30800-30814 Sentence denotes Immunoblotting
T11381 30815-30995 Sentence denotes 1×106 cells were seeded in 12-well plates, stimulated as indicated, and lysed in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific).
T11382 30996-31073 Sentence denotes Protein concentrations were determined by Bradford assay (Thermo Scientific).
T11383 31074-31194 Sentence denotes Lysates were resolved by electrophoreses on 10% SDS-polyacrylamide gels and transferred onto PVDF membranes (Millipore).
T11384 31195-31352 Sentence denotes Blots were blocked for 1 hour at room temperature and probed overnight at 4°C for pro-IL-1β (Santa Cruz), IκBα (Cell Signaling), or β-actin (Cell Signaling).
T11385 31353-31485 Sentence denotes Blots were subsequently probed with fluorescently-labeled secondary antibodies, IRDye® 680 or 800CW (LI-COR Biosciences) for 1 hour.
T11386 31486-31641 Sentence denotes Both blocking and probing steps were carried out in tris-buffered saline (G Biosciences) containing 5% bovine serum albumin and 0.1% TWEEN 20 (Calbiochem).
T11387 31642-31777 Sentence denotes Blots were imaged on a LI-COR Odyssey infrared imaging system (LI-COR Biosciences) and quantified using the included analysis software.
T11755 31779-31815 Sentence denotes Quantification of caspase-1 activity
T11756 31816-31902 Sentence denotes Bronchial epithelial cells were plated in 6-well plates at 5×105 cells/well overnight.
T11757 31903-32018 Sentence denotes Cells were primed with LPS for 5 hours and stimulated with ATP for 1 hour or stimulated with live PAO1 for 3 hours.
T11758 32019-32072 Sentence denotes PBMCs were stimulated the same day as blood donation.
T11759 32073-32301 Sentence denotes PBMCs were seeded in a 96-well plate at a density of 4.5×105 cells/well (2.5×106 cells/ml), primed with LPS for 5 hours and then stimulated with ATP for 1 hour or Poly(dA:dT) for 3 hours or stimulated with live PAO1 for 3 hours.
T11760 32302-32456 Sentence denotes Caspase-1 activity was measured using FLICA (Immunochemistry Technologies), a cell-permeable fluorescent probe (FAM-YVAD-fmk) that binds active caspase-1.
T11761 32457-32596 Sentence denotes Cells were incubated 1 hour with FLICA at 37°C and stained with PE-Cy7-conjugated anti-CD14 antibodies (eBioscience) to identify monocytes.
T11762 32597-32750 Sentence denotes The gating strategy consisted of including live cells that were CD14 positive which were subsequently analyzed for the frequency of FLICA positive cells.
T12140 32752-32777 Sentence denotes NF-κB/AP-1 Activity Assay
T12141 32778-32918 Sentence denotes Supernatants from THP-1 reporter cells were incubated with Quanti-Blue substrate (Invivogen) at 37°C and allowed to develop for 16–18 hours.
T12142 32919-33166 Sentence denotes Quanti-Blue contains a substrate for alkaline phosphatase and changes in the amount of NF-κB/AP-1 activity were quantified by optical density (λ = 655) measured using a SpectraMax 384 Plus plate reader and SoftMax Pro software (Molecular Devices).
T12298 33168-33185 Sentence denotes Bacterial strains
T12299 33186-33452 Sentence denotes P. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock. P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase.
T12300 33453-33548 Sentence denotes Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm).
T12301 33549-33674 Sentence denotes To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour.
T12302 33675-33863 Sentence denotes For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection.
T12303 33864-33964 Sentence denotes Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.
T12606 33966-33976 Sentence denotes Statistics
T12607 33977-34054 Sentence denotes All graphs display the mean ± SEM and were generated with Prism 5 (Graphpad).
T12608 34055-34176 Sentence denotes Statistical significance was determined by performing one or two-way ANOVA and the Bonferroni post-test where applicable.
T12680 6912-6952 Sentence denotes Cell stimulation and inhibitor schedule.
T12681 6953-7128 Sentence denotes Schedule outlines the timing of inhibitor addition and priming in relation to inflammasome stimulation (t = 0) for THP-1 reporter and PBMC cytokine quantification experiments.
T12682 7129-7234 Sentence denotes Inhibitor treatments and stimulations were carried out as described in the Materials and Methods section.
T12809 7279-7391 Sentence denotes Airway epithelial cells do not significantly contribute to IL-1β production in response to inflammasome stimuli.
T12810 7392-7639 Sentence denotes Control cell lines ((A) S9, (C) NuLi-1) and their corresponding CF cell lines ((B) IB3-1, and (D) CuFi-1) cells were stimulated with P. aeruginosa (MOI = 10), ATP (5 mM), or IL-1β (10 ng/ml), for the indicated times (n = 3 individual experiments).
T12811 7640-7709 Sentence denotes Cells were primed with LPS (100 ng/ml) for 4 hours where appropriate.
T12812 7710-7788 Sentence denotes Cell culture supernatants were assayed for IL-1β and IL-8 production by ELISA.
T12813 7789-7866 Sentence denotes Insert shows IL-8 secretion in response to stimulation with IL-1β (10 ng/ml).
T13116 8724-8819 Sentence denotes Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.
T13117 8820-8958 Sentence denotes S9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM).
T13118 8959-9016 Sentence denotes Cells were primed with LPS for 5 hours where appropriate.
T13119 9017-9329 Sentence denotes A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50).
T13120 9330-9423 Sentence denotes Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).
T13406 9944-10054 Sentence denotes PBMCs from CF patients and controls show similar increases in caspase-1 activity upon inflammasome activation.
T13407 10055-10244 Sentence denotes PBMCs from CF patients (n = 6) and healthy controls (n = 6) were primed with LPS (10 ng/ml) for 5 hours prior to stimulation with ATP (5 mM) for 1 hour or Poly(dA:dT) (1 µg/ml) for 3 hours.
T13408 10245-10310 Sentence denotes PBMCs were stimulated with P. aeruginosa strain PAO1 for 3 hours.
T13409 10311-10424 Sentence denotes A representative histogram of the % caspase-1-active cells is shown in (A) with the averaged values shown in (B).
T13641 11893-11958 Sentence denotes PBMCs from CF patients do not produce increased amounts of IL-1β.
T13642 11959-12212 Sentence denotes PBMCs from CF patients (n = 17–20) and healthy controls (n = 15–19) were primed with LPS (10 ng/ml) overnight and stimulated with P. aeruginosa PAO1 (MOI = 1), P. aeruginosa PAO1 lacking exsA (MOI = 1), ATP (5 mM), or Poly(dA:dT) (1 µg/ml) for 24 hours.
T13643 12213-12324 Sentence denotes P. aeruginosa lacking exsA was used as a type III secretion control in comparison with wild-type P. aeruginosa.
T13644 12325-12378 Sentence denotes Supernatants were assayed for (A) IL-1β and (B) IL-8.
T13935 14091-14194 Sentence denotes NF-κB activation potentiates the degree of IL-1β production and secretion upon inflammasome activation.
T13936 14195-14385 Sentence denotes THP-1 reporter cells were primed overnight with heat-killed P. aeruginosa and stimulated the next day with live P. aeruginosa or additional heat-killed P. aeruginosa for the times indicated.
T13937 14386-14510 Sentence denotes Cell culture supernatants were assayed for (A) NF-κB/AP-1 activity, (B) IL-8, and (C) IL-1β secretion (n = 3–6 experiments).
T13938 14511-14667 Sentence denotes Using the same stimulation method, THP-1 reporter cells were treated with Bay11-7082 (20 µM) for 1 hour prior to priming with heat-killed PAO1 or live PAO1.
T13939 14668-14779 Sentence denotes Supernatants were assayed at 24 hours for (D) NF-κB/AP-1 activity, (E) IL-8, and (F) IL-1β secretion (n = 3–5).
T13940 14780-15072 Sentence denotes PBMCs from CF patients (n = 11–15) and controls (n = 10–13) were treated with z-YVAD-fmk (20 µM) or Bay11-7082 (10 µM) and stimulated with live PAO1 (MOI = 1), ATP (5 mM), or Poly(dA:dT) (1 µg/ml) according to the schedule in Figure 1. (G) IL-1β and (H) IL-8 levels were measured at 24 hours.
T13941 15073-15228 Sentence denotes Statistical analysis was performed using two way ANOVA with Bonferroni correction for multiple comparisons. *, **, and *** signify P<0.05, 0.01, and 0.001.
T14502 15927-15997 Sentence denotes Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa.
T14503 15998-16183 Sentence denotes PMA-differentiated THP-1 cells were treated with 10 µM CFTRinh172 or 20 µM Bay11-7082 and harvested after (A) 4 hours (n = 3) or (B) 0.5, 1, and 1.5 hours (n = 3) stimulation with PAO1.
T14504 16184-16295 Sentence denotes One representative blot is shown with a graph of the averaged fluorescence intensity values over 3 experiments.
T14670 17130-17217 Sentence denotes Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.
T14671 17218-17502 Sentence denotes PBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages.
T14672 17503-17620 Sentence denotes Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours.
T14673 17621-17807 Sentence denotes THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).
T15013 7910-7934 Sentence denotes Inflammasome Activators.
T1 0-105 Sentence denotes Inflammasome-Mediated IL-1β Production in Humans with Cystic Fibrosis IL-1β Production in Cystic Fibrosis
T2 108-116 Sentence denotes Abstract
T3 117-127 Sentence denotes Background
T4 128-287 Sentence denotes Inflammation and infection are major determinants of disease severity and consequently, the quality of life and outcome for patients with cystic fibrosis (CF).
T5 288-346 Sentence denotes Interleukin-1 beta (IL-1β) is a key inflammatory mediator.
T6 347-428 Sentence denotes Secretion of biologically active IL-1β involves inflammasome-mediated processing.
T7 429-526 Sentence denotes Little is known about the contribution of IL-1β and the inflammasomes in CF inflammatory disease.
T8 527-651 Sentence denotes This study examines inflammasome-mediated IL-1β production in CF bronchial epithelial cell lines and human patients with CF.
T9 653-660 Sentence denotes Results
T10 661-886 Sentence denotes Bronchial epithelial cell lines were found to produce negligible amounts of basal or stimulated IL-1β compared to hematopoeitic cells and they did not significantly upregulate caspase-1 activity upon inflammasome stimulation.
T11 887-1091 Sentence denotes In contrast, peripheral blood mononuclear cells (PBMCs) from both CF and healthy control subjects produced large amounts of IL-1β and strongly upregulated caspase-1 activity upon inflammasome stimulation.
T12 1092-1243 Sentence denotes PBMCs from CF patients and controls displayed similar levels of caspase-1 activation and IL-1β production when stimulated with inflammasome activators.
T13 1244-1340 Sentence denotes This IL-1β production was dependent on NF-κB activity and could be enhanced by priming with LPS.
T14 1341-1502 Sentence denotes Finally, chemical inhibition of CFTR activity in control PBMCs and THP-1 cells did not significantly alter IL-1β or IL-8 production in response to P. aeruginosa.
T15 1504-1514 Sentence denotes Conclusion
T16 1515-1624 Sentence denotes Hematopoeitic cells appear to be the predominant source of inflammasome-induced pro-inflammatory IL-1β in CF.
T17 1625-1778 Sentence denotes PBMCs derived from CF subjects display preserved inflammasome activation and IL-1β secretion in response to the major CF pathogen Pseudomonas aeruginosa.
T18 1779-1954 Sentence denotes However, our data do not support the hypothesis that increased IL-1β production in CF subjects is due to an intrinsic increase in NF-κB activity through loss of CFTR function.
T19 1957-1969 Sentence denotes Introduction
T20 1970-2149 Sentence denotes Cystic fibrosis is an autosomal recessive disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as a chloride ion channel.
T21 2150-2331 Sentence denotes CF remains one of the most common lethal genetic diseases in populations of European descent with the current average lifespan of CF patients approximately 40 years of age [1], [2].
T22 2332-2455 Sentence denotes Recurrent inflammatory pulmonary exacerbation is the primary cause of lung disease progression and ultimately, death in CF.
T23 2456-2615 Sentence denotes Controlled inflammation is important for fighting infection, but in excess, it becomes destructive to host cells and to the architecture of the lungs [2], [3].
T24 2616-2748 Sentence denotes In CF, airway epithelial cells have been shown to produce an exaggerated pro-inflammatory cytokine response to stimulation [4], [5].
T25 2749-2914 Sentence denotes It is unclear whether this heightened inflammatory response is intrinsic to cells lacking CFTR or whether it is a result of chronic polymicrobial infection [6], [7].
T26 2915-3137 Sentence denotes Regardless of this controversy, identifying and targeting relevant inflammatory mediators is a critical step in developing more specific therapeutic approaches to control inflammation and improve health outcomes in CF [8].
T27 3138-3198 Sentence denotes Interleukin-1 beta (IL-1β) is a major inflammatory mediator.
T28 3199-3514 Sentence denotes Its physiological effects are diverse and potentially important to the pathogenesis of lung exacerbations in CF, including the generation of fever, the recruitment of inflammatory effector cells, the induction of other pro-inflammatory cytokines such as IL-6 and IL-8, and the shaping of T cell responses [9], [10].
T29 3515-3650 Sentence denotes Following initiation of the NF-κB signaling cascade, IL-1β is produced in the cytosol as a biologically inactive full-length pro-IL-1β.
T30 3651-3978 Sentence denotes Pro-IL-1β is subsequently converted into its active form by cytosolic protein complexes termed “inflammasomes.” Inflammasomes assemble in response to certain cellular danger signals and mediate the auto-activation of caspase-1 [9], [11], which cleaves pro-IL-1β and pro-IL-18 into their biologically active forms for secretion.
T31 3979-4028 Sentence denotes Four distinct inflammasomes have been recognized.
T32 4029-4211 Sentence denotes These are the NLRP1 [12], NLRP3 [13], [14], NLRC4 [15], [16], and AIM2 inflammasomes [17], [18], which respond to a variety of different microbial signatures and danger signals [11].
T33 4212-4347 Sentence denotes P. aeruginosa, one of the most common and clinically relevant pathogens among CF patients, activates the NLRC4 inflammasome [19], [20].
T34 4348-4499 Sentence denotes Infection with P. aeruginosa triggers an increase in levels of IL-1β, IL-6, and IL-8 in bronchoalveolar lavage fluid (BALF) from patients with CF [21].
T35 4500-4687 Sentence denotes Inflammasome responses depend on NF-κB signaling, where NF-κB is important in both the upregulation of specific inflammasome components [22], [23], as well as IL-1β expression [24], [25].
T36 4688-4782 Sentence denotes Previous studies support a role for IL-1β in the pathogenesis of CF inflammatory lung disease.
T37 4783-4968 Sentence denotes Levels of IL-1β are increased in BALF from CF patients with infection [21], [26], [27], [28] and this increase has been temporally associated with a clinical response to treatment [21].
T38 4969-5087 Sentence denotes Polymorphisms in the IL1B gene have also been associated with varying degrees of disease severity in CF patients [29].
T39 5088-5355 Sentence denotes Murine models of CFTR dysfunction have exhibited significant increases in IL-1β expression or secretion in macrophages [30], [31], and support the hypothesis that the loss of CFTR increases NF-κB activation under basal and stimulatory conditions [4], [5], [32], [33].
T40 5356-5619 Sentence denotes Finally, replacement of chloride ions with glutamate or gluconate in cell culture media increases secretion of IL-1β in response to NLRP3 stimulation by adenosine triphosphate (ATP) [34], implying an inhibitory role for extracellular chloride in NLRP3 activation.
T41 5620-5746 Sentence denotes Taken together, these data implicate the involvement of IL-1β and consequently, the inflammasomes, in CF inflammatory disease.
T42 5748-5755 Sentence denotes Results
T43 5757-5864 Sentence denotes Airway epithelial cells do not produce significant amounts of IL-1β in response to inflammasome stimulation
T44 5865-5960 Sentence denotes The inflammasomes and their respective activators examined in this study are listed in Table 1.
T45 5961-6027 Sentence denotes Cells were stimulated in accordance with the schedule in Figure 1.
T46 6028-6188 Sentence denotes In CF, airway epithelial cells have been shown to possess a hyper-inflammatory phenotype and produce an exaggerated pro-inflammatory cytokine response [4], [5].
T47 6189-6446 Sentence denotes To determine if airway epithelial cells contribute to the increased IL-1β production in patients with CF, CF and control bronchial epithelial cell lines were stimulated with the inflammasome inducers P. aeruginosa strain PAO1 (PAO1) and LPS followed by ATP.
T48 6447-6698 Sentence denotes IL-1β levels in cell culture supernatants were not greatly increased in either the CF or control cell lines (Fig. 2a–d), although a small increase in IL-1β production was detected in NuLi-1 and CuFi-1 cells, but not in S9 and IB3-1 cells, by 24 hours.
T49 6699-6867 Sentence denotes In contrast, these airway cells were highly responsive to other inflammatory stimuli, such as recombinant IL-1β, producing large quantities of IL-8 (Fig. 2a–d inserts).
T50 6868-6952 Sentence denotes 10.1371/journal.pone.0037689.g001 Figure 1 Cell stimulation and inhibitor schedule.
T51 6953-7128 Sentence denotes Schedule outlines the timing of inhibitor addition and priming in relation to inflammasome stimulation (t = 0) for THP-1 reporter and PBMC cytokine quantification experiments.
T52 7129-7234 Sentence denotes Inhibitor treatments and stimulations were carried out as described in the Materials and Methods section.
T53 7235-7391 Sentence denotes 10.1371/journal.pone.0037689.g002 Figure 2 Airway epithelial cells do not significantly contribute to IL-1β production in response to inflammasome stimuli.
T54 7392-7639 Sentence denotes Control cell lines ((A) S9, (C) NuLi-1) and their corresponding CF cell lines ((B) IB3-1, and (D) CuFi-1) cells were stimulated with P. aeruginosa (MOI = 10), ATP (5 mM), or IL-1β (10 ng/ml), for the indicated times (n = 3 individual experiments).
T55 7640-7709 Sentence denotes Cells were primed with LPS (100 ng/ml) for 4 hours where appropriate.
T56 7710-7788 Sentence denotes Cell culture supernatants were assayed for IL-1β and IL-8 production by ELISA.
T57 7789-7866 Sentence denotes Insert shows IL-8 secretion in response to stimulation with IL-1β (10 ng/ml).
T58 7867-7934 Sentence denotes 10.1371/journal.pone.0037689.t001 Table 1 Inflammasome Activators.
T59 7936-8050 Sentence denotes Airway epithelial cells do not significantly upregulate caspase-1 activity in response to inflammasome stimulation
T60 8051-8171 Sentence denotes To examine if inflammasome activation occurs in these airway cells, caspase-1 activity was quantified by flow cytometry.
T61 8172-8283 Sentence denotes There was no significant increase upon stimulation with live PAO1 or LPS+ATP at the times examined (Fig. 3a–b).
T62 8284-8543 Sentence denotes Because previous studies have indicated a role for caspase-1 in the activation of NF-κB through Toll-like receptor (TLR) signaling [35], we examined whether chemical inhibition of caspase-1 altered NF-κB-dependent IL-6 production in response to P. aeruginosa.
T63 8544-8679 Sentence denotes However, treatment with the caspase-1 inhibitor z-YVAD-fmk (YVAD) did not decrease IL-6 secretion by airway epithelial cells (Fig. 3c).
T64 8680-8819 Sentence denotes 10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.
T65 8820-8958 Sentence denotes S9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM).
T66 8959-9016 Sentence denotes Cells were primed with LPS for 5 hours where appropriate.
T67 9017-9329 Sentence denotes A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50).
T68 9330-9423 Sentence denotes Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).
T69 9425-9553 Sentence denotes CD14 positive monocytes from CF patients and controls show similar increases in caspase-1 activity upon inflammasome stimulation
T70 9554-9635 Sentence denotes Monocytes were identified in PBMC populations using CD14 as a phenotyping marker.
T71 9636-9899 Sentence denotes CD14 positive monocytes from CF patients and healthy controls showed a significant increase in caspase-1 activation upon stimulation with LPS+ATP, PAO1, and LPS+Poly(dA:dT) (Fig. 4a) but this activation was not different between CF and control subjects (Fig. 4b).
T72 9900-10054 Sentence denotes 10.1371/journal.pone.0037689.g004 Figure 4 PBMCs from CF patients and controls show similar increases in caspase-1 activity upon inflammasome activation.
T73 10055-10244 Sentence denotes PBMCs from CF patients (n = 6) and healthy controls (n = 6) were primed with LPS (10 ng/ml) for 5 hours prior to stimulation with ATP (5 mM) for 1 hour or Poly(dA:dT) (1 µg/ml) for 3 hours.
T74 10245-10310 Sentence denotes PBMCs were stimulated with P. aeruginosa strain PAO1 for 3 hours.
T75 10311-10424 Sentence denotes A representative histogram of the % caspase-1-active cells is shown in (A) with the averaged values shown in (B).
T76 10426-10520 Sentence denotes PBMCs from CF patients do not produce increased amounts of IL-1β upon inflammasome stimulation
T77 10521-10674 Sentence denotes Previous studies have shown that the loss of CFTR results in increased NF-κB activity and pro-inflammatory cytokine secretion [4], [5], [32], [36], [37].
T78 10675-10887 Sentence denotes To further examine this relationship, PBMCs from CF patients and healthy adult controls were stimulated with PAO1, LPS+ATP, and LPS+Poly(dA:dT), to activate the NLRC4, NLRP3, and AIM2 inflammasomes, respectively.
T79 10888-11030 Sentence denotes By 24 hours of stimulation, CF PBMCs did not produce increased amounts of IL-1β (Fig. 5a) or IL-8 (Fig. 5b) when compared to healthy controls.
T80 11031-11180 Sentence denotes However, we did notice a transient decrease (P<0.001) in the amount of IL-1β produced by CF cells in response to LPS+ATP at 6 hours (data not shown).
T81 11181-11379 Sentence denotes Stimulation of PBMCs with P. aeruginosa that lacks exsA (PAO1ΔexsA), a key regulator of type III secretion, produced three-fold less IL-1β compared to the parental PAO1 strain by 24 hours (Fig. 5a).
T82 11380-11490 Sentence denotes Inflammasome stimulation without priming did not result in any IL-1β production in either CF or control PBMCs.
T83 11491-11718 Sentence denotes Contrary to our hypothesis, these results indicate that PBMCs from CF patients do not display increased production of IL-1β or IL-8 with inflammasome activation nor do they suggest any increased basal or induced NF-κB activity.
T84 11719-11848 Sentence denotes These results are consistent with our observation that caspase-1 activity is not different between CF and control PBMCs (Fig. 4).
T85 11849-11958 Sentence denotes 10.1371/journal.pone.0037689.g005 Figure 5 PBMCs from CF patients do not produce increased amounts of IL-1β.
T86 11959-12212 Sentence denotes PBMCs from CF patients (n = 17–20) and healthy controls (n = 15–19) were primed with LPS (10 ng/ml) overnight and stimulated with P. aeruginosa PAO1 (MOI = 1), P. aeruginosa PAO1 lacking exsA (MOI = 1), ATP (5 mM), or Poly(dA:dT) (1 µg/ml) for 24 hours.
T87 12213-12324 Sentence denotes P. aeruginosa lacking exsA was used as a type III secretion control in comparison with wild-type P. aeruginosa.
T88 12325-12378 Sentence denotes Supernatants were assayed for (A) IL-1β and (B) IL-8.
T89 12380-12454 Sentence denotes NF-κB activation is required for IL-1β and IL-8 responses to P. aeruginosa
T90 12456-12629 Sentence denotes We confirmed the dependence of PAO1-induced IL-1β and IL-8 production on NF-κB activation using THP-1 cells expressing a reporter driven by NF-κB and AP-1 response elements.
T91 12630-12878 Sentence denotes We found that stimulation of primed THP-1 reporter cells with heat-killed PAO1 produced the highest levels of NF-κB/AP-1 activation (Fig. 6a) and this correlated with IL-8 secretion (Fig. 6b) but negligible amounts of IL-1β were secreted (Fig. 6c).
T92 12879-13045 Sentence denotes Stimulation of primed THP-1 reporter cells with live PAO1 did not significantly increase NF-κB/AP-1 activity (Fig. 6a) or IL-8 secretion (Fig. 6b) over priming alone.
T93 13046-13199 Sentence denotes However, IL-1β production was greatly augmented over primed cells stimulated with heat-killed PAO1 or unprimed cells stimulated with live PAO1 (Fig. 6c).
T94 13200-13407 Sentence denotes This confirmed that NF-κB activation alone is not sufficient for maximal IL-1β secretion, but increased priming of NF-κB is capable of augmenting IL-1β production and secretion upon inflammasome stimulation.
T95 13408-13757 Sentence denotes Dependency of these responses on NF-κB was confirmed by pharmacologic inhibition of NF-κB using the Bay11-7082 inhibitor of IκBα phosphorylation, which significantly reduced NF-κB/AP-1 activation (P<0.001) (Fig. 6d) and the subsequent production of IL-8 (P<0.01) (Fig. 6e) and IL-1β (P<0.001) (Fig. 6f) in response to both heat-killed and live PAO1.
T96 13758-13868 Sentence denotes These results were also verified in CF and control PBMCs for each inflammasome examined (P<0.001) (Fig. 6g–h).
T97 13869-14046 Sentence denotes Overall these results confirm that NF-κB is an important modulator of IL-1β production and that increased activation of NF-κB augments inflammasome-mediated production of IL-1β.
T98 14047-14194 Sentence denotes 10.1371/journal.pone.0037689.g006 Figure 6 NF-κB activation potentiates the degree of IL-1β production and secretion upon inflammasome activation.
T99 14195-14385 Sentence denotes THP-1 reporter cells were primed overnight with heat-killed P. aeruginosa and stimulated the next day with live P. aeruginosa or additional heat-killed P. aeruginosa for the times indicated.
T100 14386-14510 Sentence denotes Cell culture supernatants were assayed for (A) NF-κB/AP-1 activity, (B) IL-8, and (C) IL-1β secretion (n = 3–6 experiments).
T101 14511-14667 Sentence denotes Using the same stimulation method, THP-1 reporter cells were treated with Bay11-7082 (20 µM) for 1 hour prior to priming with heat-killed PAO1 or live PAO1.
T102 14668-14779 Sentence denotes Supernatants were assayed at 24 hours for (D) NF-κB/AP-1 activity, (E) IL-8, and (F) IL-1β secretion (n = 3–5).
T103 14780-15072 Sentence denotes PBMCs from CF patients (n = 11–15) and controls (n = 10–13) were treated with z-YVAD-fmk (20 µM) or Bay11-7082 (10 µM) and stimulated with live PAO1 (MOI = 1), ATP (5 mM), or Poly(dA:dT) (1 µg/ml) according to the schedule in Figure 1. (G) IL-1β and (H) IL-8 levels were measured at 24 hours.
T104 15073-15228 Sentence denotes Statistical analysis was performed using two way ANOVA with Bonferroni correction for multiple comparisons. *, **, and *** signify P<0.05, 0.01, and 0.001.
T105 15230-15299 Sentence denotes Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa
T106 15301-15411 Sentence denotes In addition to inhibition of NF-κB activity, Bay11-7082 can also directly inhibit the NLRP3 inflammasome [38].
T107 15412-15623 Sentence denotes To validate its use in this study as an NF-κB inhibitor, western blots for pro-IL-1β were performed alongside inhibition of CFTR activity by CFTRinh172 in response to PAO1 at 4 hours after stimulation (Fig. 7a).
T108 15624-15740 Sentence denotes Our results indicate that Bay11-7082 prevents production of pro-IL-1β whereas CFTRinh172 does not seem to affect it.
T109 15741-15882 Sentence denotes This was further corroborated by the ability Bay11-7082 to inhibit IκBα degradation at 0.5, 1, and 1.5 hours post PAO1 stimulation (Fig. 7b).
T110 15883-15997 Sentence denotes 10.1371/journal.pone.0037689.g007 Figure 7 Bay11-7082 inhibits pro-IL-1β production in response to P. aeruginosa.
T111 15998-16183 Sentence denotes PMA-differentiated THP-1 cells were treated with 10 µM CFTRinh172 or 20 µM Bay11-7082 and harvested after (A) 4 hours (n = 3) or (B) 0.5, 1, and 1.5 hours (n = 3) stimulation with PAO1.
T112 16184-16295 Sentence denotes One representative blot is shown with a graph of the averaged fluorescence intensity values over 3 experiments.
T113 16297-16384 Sentence denotes Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells
T114 16385-16507 Sentence denotes A previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34].
T115 16508-16725 Sentence denotes To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa.
T116 16726-16838 Sentence denotes Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b).
T117 16839-16977 Sentence denotes Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d).
T118 16978-17085 Sentence denotes IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.
T119 17086-17217 Sentence denotes 10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.
T120 17218-17502 Sentence denotes PBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages.
T121 17503-17620 Sentence denotes Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours.
T122 17621-17807 Sentence denotes THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).
T123 17809-17819 Sentence denotes Discussion
T124 17820-18006 Sentence denotes Levels of IL-1β are increased in the BALF of CF patients but the cellular source of this cytokine and its production in the context of targeted inflammasome activation are still unclear.
T125 18007-18176 Sentence denotes We first studied airway epithelial cells due to their role in barrier function, proximity to infection, and ability to produce high levels of pro-inflammatory cytokines.
T126 18177-18416 Sentence denotes However, we found that bronchial epithelial cells do not produce significant amounts of IL-1β and do not show a significant increase in caspase-1 activation in response to PAO1 and LPS+ATP, in comparison to hematopoeitic mononuclear cells.
T127 18417-18526 Sentence denotes Hematopoeitically-derived cells, such as monocytes and macrophages, appear to be a principal source of IL-1β.
T128 18527-18726 Sentence denotes CFTR is expressed in alveolar macrophages [32], [39] as well as in PBMCs at both the mRNA and protein level [40], [41], and its loss is frequently associated with an augmented inflammatory phenotype.
T129 18727-19110 Sentence denotes Despite our findings indicating that bronchial epithelial cells when grown in vitro are unlikely to be significantly involved in the direct production of IL-1β (Fig. 2a–d), others have shown their capacity to respond to alveolar macrophage-derived IL-1β and to amplify the inflammatory response through the induction of chemokines and recruitment of inflammatory effector cells [42].
T130 19111-19338 Sentence denotes This interaction may constitute a critical component to effective host defense and diminishing the capacity of host cells to respond to IL-1β may leave the host susceptible to infections by pathogens such as P. aeruginosa [43].
T131 19339-19485 Sentence denotes Conversely, overproduction of IL-1β can also play a key role in chronic inflammatory responses and cause damage to the lung parenchyma [44], [45].
T132 19486-19694 Sentence denotes Although we hypothesized that CF cells would secrete increased amounts of IL-1β, we found that IL-1β production in CF PBMCs was not increased upon inflammasome stimulation as compared to controls (Fig. 5a–b).
T133 19695-19924 Sentence denotes This was in contrast to a previous study from our group, which showed increased IL-1β production by CF PBMCs in response to LPS [46], although this difference may be accounted for by technical issues in stimulation time and dose.
T134 19925-20089 Sentence denotes Moreover, IL-1β production was not increased in CF PBMCs with inflammasome stimulation alone as would be anticipated if there were basal levels of NF-κB activation.
T135 20090-20319 Sentence denotes Cells deficient in CFTR are thought to exhibit an increased basal level of NF-κB activity, which leads to increased pro-inflammatory cytokine production including an increased availability of pro-IL-1β for cleavage and secretion.
T136 20320-20488 Sentence denotes This amplification of IL-1β secretion was shown by priming THP-1 monocytes and PBMCs with heat-killed P. aeruginosa or LPS prior to stimulation with live P. aeruginosa.
T137 20489-20600 Sentence denotes This dramatically increased IL-1β secretion over stimulation with live P. aeruginosa without priming (Fig. 6c).
T138 20601-20742 Sentence denotes Similarly, if CF PBMCs expressed increased basal NF-κB activity, there would be an increase in IL-1β secretion in the absence of LPS priming.
T139 20743-20819 Sentence denotes However, no increase in IL-1β was observed under basal or primed conditions.
T140 20820-20898 Sentence denotes Studies investigating the production of IL-1β have been somewhat inconsistent.
T141 20899-21160 Sentence denotes A study by Reininger et al. [43] provided evidence that human bronchial epithelial cells possessing the ΔF508 CFTR mutation had a slightly reduced capacity to produce IL-1β and lacked the ability to induce an early NF-κB activation in response to P. aeruginosa.
T142 21161-21411 Sentence denotes Conversely, a study by Kotrange et al. [31] found that murine bone marrow-derived macrophages expressing ΔF508-CFTR produced increased amounts of IL-1β when compared to macrophages expressing normal CFTR in response to Burkholderia cenocepacia K56-2.
T143 21412-21537 Sentence denotes The differentiation of monocytes into macrophages may partly account for the differences observed with the study by Kotrange.
T144 21538-21726 Sentence denotes Inflammasome-mediated IL-1β production by monocytes and PBMCs does differ from macrophages [47], [48], [49] and macrophages are found to have higher expression of CFTR over monocytes [50].
T145 21727-21910 Sentence denotes However, as monocytes and other PBMCs express CFTR [50], [51] and produce large amounts of IL-1β, they are adequate models to examine the effects of CFTR function on IL-1β production.
T146 21911-21977 Sentence denotes Other hematopoeitic cells may also contribute to IL-1β production.
T147 21978-22160 Sentence denotes For example, neutrophil counts can be significantly increased in the lungs of CF patients [52], [53], [54] and may produce mature IL-1β through caspase-1 independent mechanisms [55].
T148 22161-22260 Sentence denotes The role of NF-κB activation in inflammasome activation and IL-1β secretion is not straightforward.
T149 22261-22406 Sentence denotes Studies have revealed an essential role for NF-κB activation in the production of pro-IL-1β and inflammasome components such as NLRP3 [22], [23].
T150 22407-22599 Sentence denotes In contrast, deletion of IKKβ, a kinase essential in NF-κB activation, increases IL-1β secretion in murine macrophages [56], [57] and demonstrates a dual role for NF-κB in regulation of IL-1β.
T151 22600-22791 Sentence denotes To address this uncertainty in our experiments we also quantified IL-8, an important CF cytokine and marker of NF-κB activation [58], and found no differences between CF and control subjects.
T152 22792-22932 Sentence denotes Similarly, levels of intracellular pro-IL-1β in THP-1 cells were dependent on NF-κB activity and did not increase with CFTRinh172 treatment.
T153 22933-23081 Sentence denotes Subsequent treatment of THP-1 cells and PBMCs with the NF-κB inhibitor Bay11-7082 significantly inhibited both IL-1β and IL-8 secretion (Fig. 6d–h).
T154 23082-23173 Sentence denotes Therefore, the IL-1β and IL-8 responses observed were both dependent upon NF-κB activation.
T155 23174-23454 Sentence denotes Priming with heat-killed P. aeruginosa, like LPS, is unable to induce a strong IL-1β response as compared to live P. aeruginosa (Fig. 6c), but generated greater NF-κB/AP-1 activation (Fig. 6a) and IL-8 secretion (Fig. 6b) than live bacteria despite stimulation at equivalent MOIs.
T156 23455-23594 Sentence denotes This may be indicative of the different degree and quality of the inflammatory response generated by live as opposed to dead bacteria [59].
T157 23595-23784 Sentence denotes Potential shortcomings of these experiments include its translatability to lung disease and issues related to the hypermutability of P. aeruginosa during the evolution of chronic infection.
T158 23785-24274 Sentence denotes Although the responses measured in peripheral blood cells may not completely reflect the responses occurring in the CF lung, PBMCs have a number of useful advantages: (i) PBMCs are not subject to alterations that may emerge from long-term cell culture, cloning and immortalization, and (ii) PBMCs express a large repertoire of innate immune receptors and secrete a broad array of cytokines and chemokines allowing comprehensive analysis of the modulation of inflammatory responses by CFTR.
T159 24275-24412 Sentence denotes Consideration must also be given to the nature of P. aeruginosa infection and genotypic changes in P. aeruginosa as infection progresses.
T160 24413-24543 Sentence denotes P. aeruginosa mediates inflammasome activation through its type III secretion system (T3SS) and the NLRC4 inflammasome [19], [20].
T161 24544-24678 Sentence denotes However, clones of P. aeruginosa established during chronic infection may accumulate mutations in virulence factors such as exsA [60].
T162 24679-24961 Sentence denotes By employing a deletion mutant in exsA, the key regulator in T3SS transcription, we confirmed that the T3SS is important for IL-1β secretion (Fig. 5a–b), and that depending on the adaptation in type III secretion, the host IL-1β response may be up or downregulated [19], [60], [61].
T163 24962-25179 Sentence denotes In conclusion, our data are consistent with a role for hematopoietic cells, not airway epithelial cells, as the major source of inflammasome-mediated IL-1β production in the lungs in response to ATP and P. aeruginosa.
T164 25180-25318 Sentence denotes Furthermore, we find little evidence to support an increased IL-1β inflammatory response to NF-κB/Inflammasome stimulation in CF patients.
T165 25319-25533 Sentence denotes Further studies are warranted to determine if adaptations of P. aeruginosa during the course of chronic lung infection alters inflammasome activation, and whether this can be correlated with disease severity in CF.
T166 25535-25556 Sentence denotes Materials and Methods
T167 25558-25574 Sentence denotes Ethics Statement
T168 25575-25701 Sentence denotes Blood samples were obtained with informed written consent from control subjects and CF patients at the BC Children's Hospital.
T169 25702-25770 Sentence denotes Consent was obtained for children by their parent or legal guardian.
T170 25771-25854 Sentence denotes Subjects 7 years of age and older were required to provide informed assent as well.
T171 25855-25929 Sentence denotes Protocols were approved by the Clinical Research Ethics Board (H09-01192).
T172 25931-25943 Sentence denotes Cell Culture
T173 25944-26056 Sentence denotes CF (IB3-1 and CuFi-1) and control (S9 and NuLi-1) cells were obtained from the American Type Culture Collection.
T174 26057-26195 Sentence denotes IB3-1 cells were derived from a patient expressing the ΔF508 and W1282X mutations and CuFi-1 were derived from a ΔF508 homozygous patient.
T175 26196-26379 Sentence denotes S9 cells are IB3-1 cells that have been transfected with CFTR using an adeno-associated viral vector and NuLi-1 cells were derived from a patient possessing a wild-type CFTR genotype.
T176 26380-26510 Sentence denotes THP1-XBlue cells stably express a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB and AP-1 (Invivogen).
T177 26511-26601 Sentence denotes Cells were cultured as recommended by their respective suppliers using standard protocols.
T178 26602-26760 Sentence denotes S9 and IB3-1 cells were cultured in basal LHC-8 (Invitrogen) supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T179 26761-27023 Sentence denotes NuLi-1/CuFi-1 cells were cultured in BEBM serum-free medium (Lonza) with supplement bullet kit (EGF, hydrocortisone, bovine pituitary extract, transferrin, bovine insulin, triiodothyronine, epinephrine, retinoic acid), 2 mM L-glutamine, and 1 mM sodium pyruvate.
T180 27024-27183 Sentence denotes PBMCs from CF patients and controls were cultured in RPMI-1640 (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine, and 1 mM sodium pyruvate (complete RPMI).
T181 27184-27332 Sentence denotes THP1-XBlue cells were cultured in complete RPMI with the addition of zeocin (100 µg/ml) to select for cells expressing the SEAP NF-κB/AP-1 reporter.
T182 27333-27516 Sentence denotes Prior to stimulation, bronchial epithelial cell lines were plated in coated [62] 96-well plates (BD Biosciences) at 3×104 cells/well unless indicated, and allowed to adhere overnight.
T183 27517-27694 Sentence denotes Plates for S9 and IB3-1 stimulations were coated in a mixture of bovine serum albumin (100 µg/ml), fibronectin (10 µg/ml), and bovine collage type I (30 µg/ml) (BD Biosciences).
T184 27695-27785 Sentence denotes Plates for NuLi-1 and CuFi-1 were coated with collagen type IV (60 µg/ml) (Sigma Aldrich).
T185 27786-27886 Sentence denotes PBMCs were plated in 96-well plates at a density of 1.5×105 cells/well in 200 µl (7.5×105 cells/mL).
T186 27887-28106 Sentence denotes THP-1 reporter cells were differentiated into a macrophage-like phenotype using 50 ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich) for 24 hours at a density of 1×105 cells/well in 200 µl (5×105 cells/ml).
T187 28107-28194 Sentence denotes Cells were washed with PBS and allowed to rest a further 42 hours prior to stimulation.
T188 28196-28224 Sentence denotes CF and control subject PBMCs
T189 28225-28384 Sentence denotes The diagnosis of CF was established by typical clinical features, increased sweat chloride concentrations (>60 mmol/l), and detection of CF-inducing mutations.
T190 28385-28576 Sentence denotes All patients with CF were clinically stable at the time of blood donation, and we excluded any subjects who were receiving systemic corticosteroids due to potential immunomodulatory activity.
T191 28577-28635 Sentence denotes Control samples were provided by healthy adult volunteers.
T192 28636-28846 Sentence denotes In previously published work we have demonstrated that TLR-mediated inflammatory responses are stable in humans from birth to 60 years old [63], therefore did not age-match the CF patients and control subjects.
T193 28847-29019 Sentence denotes Peripheral blood was collected in sodium heparin tubes (BD Biosciences) and PBMCs were isolated using density gradient centrifugation on Ficoll-Paque™ Plus (GE Healthcare).
T194 29020-29114 Sentence denotes The layer containing PBMCs was isolated, washed twice in PBS and resuspended in complete RPMI.
T195 29115-29217 Sentence denotes Cells were enumerated by trypan blue exclusion using the Countess automated cell counter (Invitrogen).
T196 29218-29377 Sentence denotes For derivation of macrophages from monocytes, monocytes were allowed to adhere to plastic for 2 hours in RPMI 1640 after which non-adherent cells were removed.
T197 29378-29480 Sentence denotes Monocytes were allowed to differentiate in RPMI 1640 supplemented with 10% human AB serum for 10 days.
T198 29482-29526 Sentence denotes Cell stimulation and cytokine quantification
T199 29527-29619 Sentence denotes Bronchial epithelial cells were plated and allowed to adhere overnight prior to stimulation.
T200 29620-29765 Sentence denotes Bronchial epithelial cells were rested or primed with LPS for 5 hours and stimulated with live P. aeruginosa PAO1 or ATP for the times indicated.
T201 29766-29888 Sentence denotes PBMCs and THP-1 reporter cells were either rested or primed with LPS (Invivogen) or heat-killed PAO1 overnight (16 hours).
T202 29889-30040 Sentence denotes The next day the cells were challenged with live PAO1, PAO1ΔexsA, ATP (Invivogen), or Poly(dA:dT) (Sigma Aldrich) for the times indicated (see Fig. 1).
T203 30041-30206 Sentence denotes For stimulations with Poly(dA:dT), lipofectamine LTX was used at a 1∶1 (w∶v) ratio of µg of DNA to µl of lipofectamine and was mixed 30 minutes prior to stimulation.
T204 30207-30296 Sentence denotes The NF-κB inhibitor Bay11-7082 (Invivogen) was added to cultures 1 hour prior to priming.
T205 30297-30386 Sentence denotes If no priming was involved, inhibitor was added 1 hour prior to inflammasome stimulation.
T206 30387-30498 Sentence denotes The CFTR inhibitor CFTRinh172 (Sigma Aldrich) was added to cultures 18 hours prior to inflammasome stimulation.
T207 30499-30609 Sentence denotes The caspase-1 inhibitor z-YVAD-fmk (Biovision) was added to cultures 1 hour prior to inflammasome stimulation.
T208 30610-30658 Sentence denotes Supernatants were collected and stored at −20°C.
T209 30659-30798 Sentence denotes Cytokines released into supernatants from PBMCs stimulated with inflammasome activators were quantified using sandwich ELISA (eBioscience).
T210 30800-30814 Sentence denotes Immunoblotting
T211 30815-30995 Sentence denotes 1×106 cells were seeded in 12-well plates, stimulated as indicated, and lysed in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific).
T212 30996-31073 Sentence denotes Protein concentrations were determined by Bradford assay (Thermo Scientific).
T213 31074-31194 Sentence denotes Lysates were resolved by electrophoreses on 10% SDS-polyacrylamide gels and transferred onto PVDF membranes (Millipore).
T214 31195-31352 Sentence denotes Blots were blocked for 1 hour at room temperature and probed overnight at 4°C for pro-IL-1β (Santa Cruz), IκBα (Cell Signaling), or β-actin (Cell Signaling).
T215 31353-31485 Sentence denotes Blots were subsequently probed with fluorescently-labeled secondary antibodies, IRDye® 680 or 800CW (LI-COR Biosciences) for 1 hour.
T216 31486-31641 Sentence denotes Both blocking and probing steps were carried out in tris-buffered saline (G Biosciences) containing 5% bovine serum albumin and 0.1% TWEEN 20 (Calbiochem).
T217 31642-31777 Sentence denotes Blots were imaged on a LI-COR Odyssey infrared imaging system (LI-COR Biosciences) and quantified using the included analysis software.
T218 31779-31815 Sentence denotes Quantification of caspase-1 activity
T219 31816-31902 Sentence denotes Bronchial epithelial cells were plated in 6-well plates at 5×105 cells/well overnight.
T220 31903-32018 Sentence denotes Cells were primed with LPS for 5 hours and stimulated with ATP for 1 hour or stimulated with live PAO1 for 3 hours.
T221 32019-32072 Sentence denotes PBMCs were stimulated the same day as blood donation.
T222 32073-32301 Sentence denotes PBMCs were seeded in a 96-well plate at a density of 4.5×105 cells/well (2.5×106 cells/ml), primed with LPS for 5 hours and then stimulated with ATP for 1 hour or Poly(dA:dT) for 3 hours or stimulated with live PAO1 for 3 hours.
T223 32302-32456 Sentence denotes Caspase-1 activity was measured using FLICA (Immunochemistry Technologies), a cell-permeable fluorescent probe (FAM-YVAD-fmk) that binds active caspase-1.
T224 32457-32596 Sentence denotes Cells were incubated 1 hour with FLICA at 37°C and stained with PE-Cy7-conjugated anti-CD14 antibodies (eBioscience) to identify monocytes.
T225 32597-32750 Sentence denotes The gating strategy consisted of including live cells that were CD14 positive which were subsequently analyzed for the frequency of FLICA positive cells.
T226 32752-32777 Sentence denotes NF-κB/AP-1 Activity Assay
T227 32778-32918 Sentence denotes Supernatants from THP-1 reporter cells were incubated with Quanti-Blue substrate (Invivogen) at 37°C and allowed to develop for 16–18 hours.
T228 32919-33166 Sentence denotes Quanti-Blue contains a substrate for alkaline phosphatase and changes in the amount of NF-κB/AP-1 activity were quantified by optical density (λ = 655) measured using a SpectraMax 384 Plus plate reader and SoftMax Pro software (Molecular Devices).
T229 33168-33185 Sentence denotes Bacterial strains
T230 33186-33287 Sentence denotes P. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock.
T231 33288-33452 Sentence denotes P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase.
T232 33453-33548 Sentence denotes Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm).
T233 33549-33674 Sentence denotes To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour.
T234 33675-33863 Sentence denotes For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection.
T235 33864-33964 Sentence denotes Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.
T236 33966-33976 Sentence denotes Statistics
T237 33977-34054 Sentence denotes All graphs display the mean ± SEM and were generated with Prism 5 (Graphpad).
T238 34055-34176 Sentence denotes Statistical significance was determined by performing one or two-way ANOVA and the Bonferroni post-test where applicable.

2_test

Id Subject Object Predicate Lexical cue
22649552-18252915-88306670 2328-2329 18252915 denotes 2
22649552-18252915-88306671 2607-2608 18252915 denotes 2
22649552-11956063-88306672 2612-2613 11956063 denotes 3
22649552-10970832-88306673 2740-2741 10970832 denotes 4
22649552-11404248-88306674 2745-2746 11404248 denotes 5
22649552-16825601-88306675 2906-2907 16825601 denotes 6
22649552-7697269-88306676 2911-2912 7697269 denotes 7
22649552-15381503-88306677 3134-3135 15381503 denotes 8
22649552-20086235-88306678 3510-3512 20086235 denotes 10
22649552-21350580-88306679 3884-3886 21350580 denotes 11
22649552-12191486-88306680 4050-4052 12191486 denotes 12
22649552-15030775-88306681 4062-4064 15030775 denotes 13
22649552-16407890-88306682 4068-4070 16407890 denotes 14
22649552-15190255-88306683 4080-4082 15190255 denotes 15
22649552-16648853-88306684 4086-4088 16648853 denotes 16
22649552-19158676-88306685 4115-4117 19158676 denotes 17
22649552-19158675-88306686 4121-4123 19158675 denotes 18
22649552-21350580-88306687 4207-4209 21350580 denotes 11
22649552-18070936-88306688 4337-4339 18070936 denotes 19
22649552-19010992-88306689 4495-4497 19010992 denotes 21
22649552-19570822-88306690 4637-4639 19570822 denotes 22
22649552-16301671-88306691 4643-4645 16301671 denotes 23
22649552-8021507-88306692 4677-4679 8021507 denotes 24
22649552-8413223-88306693 4683-4685 8413223 denotes 25
22649552-19010992-88306694 4854-4856 19010992 denotes 21
22649552-8520783-88306695 4860-4862 8520783 denotes 26
22649552-10515411-88306696 4866-4868 10515411 denotes 27
22649552-16208679-88306697 4872-4874 16208679 denotes 28
22649552-19010992-88306698 4964-4966 19010992 denotes 21
22649552-19431193-88306699 5083-5085 19431193 denotes 29
22649552-21178113-88306700 5214-5216 21178113 denotes 31
22649552-10970832-88306701 5335-5336 10970832 denotes 4
22649552-11404248-88306702 5340-5341 11404248 denotes 5
22649552-20543983-88306703 5345-5347 20543983 denotes 32
22649552-18776130-88306704 5351-5353 18776130 denotes 33
22649552-16301672-88306705 5539-5541 16301672 denotes 34
22649552-10970832-88306706 6180-6181 10970832 denotes 4
22649552-11404248-88306707 6185-6186 11404248 denotes 5
22649552-17360653-88306708 8416-8418 17360653 denotes 35
22649552-10970832-88306709 10648-10649 10970832 denotes 4
22649552-11404248-88306710 10653-10654 11404248 denotes 5
22649552-20543983-88306711 10658-10660 20543983 denotes 32
22649552-19247502-88306712 10664-10666 19247502 denotes 36
22649552-20644644-88306713 10670-10672 20644644 denotes 37
22649552-20093358-88306714 15407-15409 20093358 denotes 38
22649552-16301672-88306715 16503-16505 16301672 denotes 34
22649552-20543983-88306716 18570-18572 20543983 denotes 32
22649552-16921366-88306717 18576-18578 16921366 denotes 39
22649552-20102408-88306718 18642-18644 20102408 denotes 41
22649552-21270399-88306719 19106-19108 21270399 denotes 42
22649552-17283089-88306720 19334-19336 17283089 denotes 43
22649552-17992263-88306721 19475-19477 17992263 denotes 44
22649552-18931327-88306722 19481-19483 18931327 denotes 45
22649552-18490781-88306723 19824-19826 18490781 denotes 46
22649552-17283089-88306724 20928-20930 17283089 denotes 43
22649552-21178113-88306725 21201-21203 21178113 denotes 31
22649552-20495003-88306726 21630-21632 20495003 denotes 47
22649552-19104081-88306727 21636-21638 19104081 denotes 48
22649552-21625641-88306728 21722-21724 21625641 denotes 50
22649552-21625641-88306729 21779-21781 21625641 denotes 50
22649552-21811577-88306730 21785-21787 21811577 denotes 51
22649552-8049828-88306731 22069-22071 8049828 denotes 52
22649552-17526676-88306732 22075-22077 17526676 denotes 53
22649552-7697234-88306733 22081-22083 7697234 denotes 54
22649552-21333600-88306734 22156-22158 21333600 denotes 55
22649552-19570822-88306735 22396-22398 19570822 denotes 22
22649552-16301671-88306736 22402-22404 16301671 denotes 23
22649552-17803913-88306737 22527-22529 17803913 denotes 56
22649552-21533069-88306738 22533-22535 21533069 denotes 57
22649552-8413215-88306739 22729-22731 8413215 denotes 58
22649552-21602824-88306740 23590-23592 21602824 denotes 59
22649552-18070936-88306741 24533-24535 18070936 denotes 19
22649552-16687478-88306742 24674-24676 16687478 denotes 60
22649552-18070936-88306743 24945-24947 18070936 denotes 19
22649552-16687478-88306744 24951-24953 16687478 denotes 60
22649552-11372029-88306745 24957-24959 11372029 denotes 61
22649552-21068401-88306746 27410-27412 21068401 denotes 62
22649552-17157070-88306747 28776-28778 17157070 denotes 63
T85898 2328-2329 18252915 denotes 2
T41701 2607-2608 18252915 denotes 2
T48945 2612-2613 11956063 denotes 3
T51403 2740-2741 10970832 denotes 4
T2050 2745-2746 11404248 denotes 5
T88634 2906-2907 16825601 denotes 6
T27899 2911-2912 7697269 denotes 7
T3751 3134-3135 15381503 denotes 8
T51662 3510-3512 20086235 denotes 10
T31735 3884-3886 21350580 denotes 11
T62254 4050-4052 12191486 denotes 12
T56249 4062-4064 15030775 denotes 13
T8344 4068-4070 16407890 denotes 14
T82915 4080-4082 15190255 denotes 15
T37753 4086-4088 16648853 denotes 16
T23506 4115-4117 19158676 denotes 17
T22148 4121-4123 19158675 denotes 18
T7622 4207-4209 21350580 denotes 11
T14488 4337-4339 18070936 denotes 19
T39745 4495-4497 19010992 denotes 21
T12002 4637-4639 19570822 denotes 22
T14767 4643-4645 16301671 denotes 23
T8246 4677-4679 8021507 denotes 24
T78036 4683-4685 8413223 denotes 25
T76824 4854-4856 19010992 denotes 21
T94729 4860-4862 8520783 denotes 26
T68481 4866-4868 10515411 denotes 27
T73546 4872-4874 16208679 denotes 28
T83481 4964-4966 19010992 denotes 21
T45701 5083-5085 19431193 denotes 29
T92061 5214-5216 21178113 denotes 31
T91667 5335-5336 10970832 denotes 4
T27983 5340-5341 11404248 denotes 5
T42884 5345-5347 20543983 denotes 32
T19477 5351-5353 18776130 denotes 33
T60798 5539-5541 16301672 denotes 34
T36864 6180-6181 10970832 denotes 4
T29257 6185-6186 11404248 denotes 5
T60341 8416-8418 17360653 denotes 35
T35806 10648-10649 10970832 denotes 4
T78747 10653-10654 11404248 denotes 5
T26859 10658-10660 20543983 denotes 32
T18939 10664-10666 19247502 denotes 36
T3744 10670-10672 20644644 denotes 37
T78032 15407-15409 20093358 denotes 38
T11221 16503-16505 16301672 denotes 34
T58856 18570-18572 20543983 denotes 32
T18679 18576-18578 16921366 denotes 39
T89454 18642-18644 20102408 denotes 41
T84739 19106-19108 21270399 denotes 42
T9340 19334-19336 17283089 denotes 43
T72228 19475-19477 17992263 denotes 44
T75025 19481-19483 18931327 denotes 45
T77502 19824-19826 18490781 denotes 46
T33980 20928-20930 17283089 denotes 43
T29693 21201-21203 21178113 denotes 31
T79992 21630-21632 20495003 denotes 47
T13570 21636-21638 19104081 denotes 48
T83784 21722-21724 21625641 denotes 50
T57463 21779-21781 21625641 denotes 50
T43269 21785-21787 21811577 denotes 51
T37294 22069-22071 8049828 denotes 52
T99145 22075-22077 17526676 denotes 53
T81293 22081-22083 7697234 denotes 54
T66801 22156-22158 21333600 denotes 55
T71344 22396-22398 19570822 denotes 22
T25618 22402-22404 16301671 denotes 23
T21084 22527-22529 17803913 denotes 56
T94273 22533-22535 21533069 denotes 57
T70446 22729-22731 8413215 denotes 58
T45393 23590-23592 21602824 denotes 59
T64607 24533-24535 18070936 denotes 19
T17093 24674-24676 16687478 denotes 60
T95583 24945-24947 18070936 denotes 19
T46177 24951-24953 16687478 denotes 60
T21545 24957-24959 11372029 denotes 61
T53749 27410-27412 21068401 denotes 62
T28787 28776-28778 17157070 denotes 63