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PMC:3342329 / 29851-31727 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T14927 1366-1375 NN denotes detection
T14926 1362-1365 DT denotes the
T14925 1358-1361 IN denotes For
T14924 1348-1356 NN denotes antibody
T14923 1340-1347 JJ denotes primary
T14922 1336-1339 DT denotes the
T14921 1327-1335 VB denotes omitting
T14920 1324-1326 IN denotes by
T14919 1312-1323 NN denotes experiments
T14918 1308-1311 DT denotes all
T14917 1305-1307 IN denotes in
T14916 1296-1304 VB denotes included
T14915 1292-1295 VB denotes was
T14914 1284-1291 NN denotes control
T14913 1275-1283 JJ denotes negative
T14912 1273-1274 DT denotes A
T14911 1270-1271 -RRB- denotes )
T14910 1265-1270 NNP denotes Japan
T14909 1263-1264 -COMMA- denotes ,
T14908 1258-1263 NNP denotes Tokyo
T14907 1256-1257 -COMMA- denotes ,
T14906 1249-1256 NNP denotes Olympus
T14905 1247-1248 -COLON- denotes ;
T14904 1230-1247 NN denotes magnification×200
T14903 1229-1230 -LRB- denotes (
T14902 1218-1228 NN denotes microscope
T14901 1212-1217 JJ denotes light
T14900 1210-1211 DT denotes a
T14899 1204-1209 IN denotes under
T14898 1193-1203 NN denotes evaluation
T14897 1189-1192 CC denotes and
T14896 1187-1188 -COMMA- denotes ,
T14895 1177-1187 JJ denotes aqua-mount
T14894 1174-1176 IN denotes in
T14893 1165-1173 VB denotes mounting
T14892 1163-1164 -COMMA- denotes ,
T14891 1152-1163 NN denotes hematoxylin
T14890 1147-1151 IN denotes with
T14889 1131-1146 VB denotes counterstaining
T14888 1128-1130 IN denotes by
T14887 1119-1127 VB denotes followed
T14886 1117-1118 -COMMA- denotes ,
T14885 1109-1117 NN denotes peroxide
T14884 1105-1108 CC denotes and
T14883 1103-1104 -RRB- denotes )
T14882 1100-1103 NN denotes DAB
T14881 1099-1100 -LRB- denotes (
T14880 1082-1098 NN denotes diaminobenzidine
T14879 1077-1081 IN denotes with
T14878 1067-1076 VB denotes developed
T14877 1063-1066 VB denotes was
T14876 1054-1062 NN denotes reaction
T14875 1043-1053 NN denotes peroxidase
T14874 1039-1042 DT denotes the
T14873 1035-1038 CC denotes and
T14872 1028-1034 VB denotes washed
T14871 1023-1027 VB denotes were
T14870 1016-1022 NN denotes slides
T14869 1012-1015 DT denotes The
T14868 1009-1010 -RRB- denotes )
T14867 1006-1009 NNP denotes USA
T14866 1004-1005 -COMMA- denotes ,
T14865 1002-1004 NNP denotes CA
T14864 1000-1001 -COMMA- denotes ,
T14863 990-1000 NNP denotes Burlingame
T14862 988-989 -COMMA- denotes ,
T14861 976-988 NNP denotes Laboratories
T14860 969-975 NNP denotes Vector
T14859 967-968 -COLON- denotes ;
T14858 964-967 NN denotes kit
T14857 960-963 NN denotes ABC
T14856 959-960 -LRB- denotes (
T14855 951-958 NN denotes complex
T14854 940-950 NN denotes peroxidase
T14853 933-939 NN denotes biotin
T14852 926-932 NN denotes avidin
T14851 922-925 DT denotes the
T14850 919-921 IN denotes of
T14849 910-918 NN denotes addition
T14848 906-909 DT denotes the
T14847 903-905 IN denotes by
T14846 894-902 VB denotes followed
T14845 892-893 -COMMA- denotes ,
T14844 889-892 NNP denotes PBS
T14843 886-888 IN denotes in
T14842 879-885 VB denotes washed
T14841 874-878 VB denotes were
T14840 867-873 NN denotes slides
T14839 863-866 DT denotes The
T14838 860-861 NN denotes h
T14837 858-859 CD denotes 2
T14836 854-857 IN denotes for
T14835 843-853 NN denotes antibodies
T14834 831-842 JJ denotes anti-rabbit
T14833 827-830 CC denotes and
T14832 816-826 JJ denotes anti-mouse
T14831 803-815 VB denotes biotinylated
T14830 798-802 IN denotes with
T14829 788-797 VB denotes incubated
T14828 784-787 CC denotes and
T14827 780-783 NNP denotes PBS
T14826 777-779 IN denotes in
T14825 772-776 DT denotes each
T14824 768-771 NN denotes min
T14823 766-767 CD denotes 5
T14822 762-765 IN denotes for
T14821 756-761 NN denotes times
T14820 750-755 CD denotes three
T14819 743-749 VB denotes washed
T14818 738-742 VB denotes were
T14817 731-737 NN denotes slides
T14816 727-730 DT denotes the
T14815 725-726 -COMMA- denotes ,
T14814 715-725 RB denotes Thereafter
T14813 702-713 NN denotes temperature
T14812 697-701 NN denotes room
T14811 694-696 IN denotes at
T14810 692-693 NN denotes h
T14809 690-691 CD denotes 4
T14808 686-689 IN denotes for
T14807 680-685 NN denotes serum
T14806 671-679 VB denotes blocking
T14805 668-670 IN denotes in
T14804 657-667 NN denotes antibodies
T14803 646-656 JJ denotes monoclonal
T14802 644-645 -RRB- denotes )
T14801 636-644 NN denotes dilution
T14800 630-635 CD denotes 1∶200
T14799 628-629 -COMMA- denotes ,
T14798 624-628 NN denotes PCNA
T14797 623-624 -LRB- denotes (
T14796 615-622 NN denotes antigen
T14795 607-614 JJ denotes nuclear
T14794 602-606 NN denotes cell
T14793 588-601 VB denotes proliferating
T14792 577-587 JJ denotes anti-mouse
T14791 571-576 NN denotes mouse
T14790 563-570 JJ denotes primary
T14789 558-562 IN denotes with
T14788 548-557 VB denotes incubated
T14787 544-547 CC denotes and
T14786 542-543 -COMMA- denotes ,
T14785 535-542 VB denotes blotted
T14784 533-534 -COMMA- denotes ,
T14783 530-533 NNP denotes PBS
T14782 527-529 IN denotes in
T14781 519-526 VB denotes diluted
T14780 513-518 NN denotes serum
T14779 507-512 NN denotes horse
T14778 500-506 JJ denotes normal
T14777 498-499 NN denotes %
T14776 497-498 CD denotes 3
T14775 492-496 IN denotes with
T14774 488-491 NN denotes min
T14773 485-487 CD denotes 30
T14772 481-484 IN denotes for
T14771 473-480 VB denotes blocked
T14770 468-472 VB denotes were
T14769 459-467 NN denotes sections
T14768 455-458 DT denotes The
T14767 450-453 NN denotes min
T14766 448-449 CD denotes 5
T14765 444-447 IN denotes for
T14764 440-443 NNP denotes PBS
T14763 437-439 IN denotes in
T14762 428-436 VB denotes clearing
T14761 425-427 IN denotes by
T14760 416-424 VB denotes followed
T14759 414-415 -COMMA- denotes ,
T14758 411-414 NN denotes min
T14757 408-410 CD denotes 15
T14756 404-407 IN denotes for
T14755 395-403 NN denotes methanol
T14754 392-394 IN denotes in
T14753 383-391 NN denotes peroxide
T14752 374-382 NN denotes hydrogen
T14751 372-373 NN denotes %
T14750 371-372 CD denotes 3
T14749 368-370 IN denotes in
T14748 357-367 NN denotes incubation
T14747 354-356 IN denotes by
T14746 345-353 VB denotes quenched
T14745 341-344 VB denotes was
T14744 332-340 NN denotes activity
T14743 321-331 NN denotes peroxidase
T14742 310-320 JJ denotes Endogenous
T14741 299-308 NN denotes retrieval
T14740 291-298 NN denotes antigen
T14739 277-290 JJ denotes heat-mediated
T14738 274-276 TO denotes to
T14737 264-273 VB denotes subjected
T14736 259-263 RB denotes then
T14735 255-258 CC denotes and
T14734 253-254 -COMMA- denotes ,
T14733 248-253 NN denotes water
T14732 238-247 JJ denotes distilled
T14731 235-237 IN denotes in
T14730 228-234 VB denotes washed
T14729 226-227 -COMMA- denotes ,
T14728 216-226 VB denotes rehydrated
T14727 212-215 CC denotes and
T14726 197-211 VB denotes deparaffinized
T14725 192-196 VB denotes were
T14724 183-191 NN denotes sections
T14723 165-182 JJ denotes paraffin-embedded
T14722 161-164 DT denotes The
T14721 139-159 NN denotes immunohistochemistry
T14720 136-138 IN denotes by
T14719 127-135 VB denotes analyzed
T14718 123-126 CC denotes and
T14717 121-122 NN denotes E
T14716 120-121 CC denotes &
T14715 119-120 NN denotes H
T14714 114-118 IN denotes with
T14713 106-113 VB denotes stained
T14712 101-105 VB denotes were
T15022 1874-1875 -RRB- denotes )
T15021 1864-1874 NN denotes microscope
T15020 1860-1863 NN denotes DAS
T15019 1859-1860 -LRB- denotes (
T15018 1848-1858 NN denotes microscopy
T15017 1835-1847 NN denotes fluorescence
T15016 1831-1834 IN denotes for
T15015 1817-1830 JJ denotes cover-slipped
T15014 1813-1816 CC denotes and
T15013 1811-1812 -COMMA- denotes ,
T15012 1805-1811 NN denotes slides
T15011 1802-1804 IN denotes on
T15010 1794-1801 VB denotes mounted
T15009 1792-1793 -COMMA- denotes ,
T15008 1786-1792 VB denotes rinsed
T15007 1781-1785 VB denotes were
T15006 1772-1780 NN denotes sections
T15005 1768-1771 DT denotes the
T15004 1766-1767 -COMMA- denotes ,
T15003 1759-1766 NNP denotes Finally
T15002 1753-1758 NNP denotes 37°C.
T15001 1750-1752 IN denotes at
T15000 1746-1749 NN denotes min
T14999 1743-1745 CD denotes 15
T14998 1739-1742 IN denotes for
T14997 1737-1738 -RRB- denotes )
T14996 1733-1737 NN denotes DAPI
T14995 1732-1733 -LRB- denotes (
T14994 1705-1731 NN denotes 6-diamidino-2-phenylindole
T14993 1704-1705 -COMMA- denotes ,
T14992 1702-1704 NN denotes 4′
T14991 1697-1701 IN denotes with
T14990 1687-1696 VB denotes incubated
T14989 1682-1686 VB denotes were
T14988 1673-1681 NN denotes sections
T14987 1669-1672 DT denotes the
T14986 1667-1668 -COMMA- denotes ,
T14985 1663-1667 RB denotes Next
T14984 1649-1661 NN denotes instructions
T14983 1634-1648 NN denotes manufacturer’s
T14982 1630-1633 DT denotes the
T14981 1627-1629 TO denotes to
T14980 1617-1626 VB denotes according
T14979 1615-1616 -COMMA- denotes ,
T14978 1611-1615 DT denotes each
T14977 1607-1610 NN denotes min
T14976 1605-1606 CD denotes 5
T14975 1601-1604 IN denotes for
T14974 1597-1600 NNP denotes PBS
T14973 1595-1596 NN denotes M
T14972 1591-1594 CD denotes 0.1
T14971 1588-1590 IN denotes in
T14970 1582-1587 NN denotes times
T14969 1576-1581 CD denotes three
T14968 1569-1575 VB denotes washed
T14967 1564-1568 RB denotes then
T14966 1560-1563 CC denotes and
T14965 1558-1559 -COMMA- denotes ,
T14964 1554-1558 NN denotes 37°C
T14963 1551-1553 IN denotes at
T14962 1549-1550 NN denotes h
T14961 1547-1548 CD denotes 1
T14960 1543-1546 IN denotes for
T14959 1541-1542 -RRB- denotes )
T14958 1539-1541 NN denotes µl
T14957 1536-1538 CD denotes 60
T14956 1535-1536 -LRB- denotes (
T14955 1526-1534 NN denotes solution
T14954 1519-1525 NN denotes enzyme
T14953 1515-1518 CC denotes and
T14952 1513-1514 -RRB- denotes )
T14951 1511-1513 NN denotes µl
T14950 1507-1510 CD denotes 540
T14949 1506-1507 -LRB- denotes (
T14948 1497-1505 NN denotes solution
T14947 1488-1496 NN denotes labeling
T14946 1484-1487 DT denotes the
T14945 1481-1483 IN denotes of
T14944 1473-1480 NN denotes mixture
T14943 1471-1472 DT denotes a
T14942 1468-1470 IN denotes in
T14941 1458-1467 VB denotes incubated
T14940 1453-1457 VB denotes were
T14939 1444-1452 NN denotes sections
T14938 1426-1443 JJ denotes paraffin-embedded
T14937 1422-1425 DT denotes the
T14936 1420-1421 -COMMA- denotes ,
T14935 1413-1420 NN denotes tissues
T14934 1407-1412 NN denotes tumor
T14933 1403-1406 DT denotes the
T14932 1400-1402 IN denotes in
T14931 1394-1399 NN denotes death
T14930 1389-1393 NN denotes cell
T14929 1379-1388 JJ denotes apoptotic
T14928 1376-1378 IN denotes of
T14711 99-100 -RRB- denotes )
T14710 94-99 JJ denotes thick
T14709 91-93 NN denotes µm
T14708 89-90 CD denotes 4
T14707 88-89 -LRB- denotes (
T14706 79-87 NN denotes Sections
T14705 66-77 NN denotes examination
T14704 62-65 IN denotes for
T14703 53-61 NN denotes paraffin
T14702 50-52 IN denotes in
T14701 41-49 VB denotes embedded
T14700 37-40 CC denotes and
T14699 28-36 NN denotes formalin
T14698 25-27 IN denotes in
T14697 19-24 VB denotes fixed
T14696 14-18 VB denotes were
T14695 4-13 NN denotes specimens
T14694 0-3 DT denotes All
R10220 T14695 T14694 arg1Of specimens,All
R10221 T14695 T14696 arg1Of specimens,were
R10222 T14695 T14697 arg2Of specimens,fixed
R10223 T14695 T14701 arg2Of specimens,embedded
R10224 T14697 T14698 arg1Of fixed,in
R10225 T14697 T14700 arg1Of fixed,and
R10226 T14699 T14698 arg2Of formalin,in
R10227 T14700 T14696 arg2Of and,were
R10228 T14701 T14700 arg2Of embedded,and
R10229 T14701 T14702 arg1Of embedded,in
R10230 T14703 T14702 arg2Of paraffin,in
R10231 T14703 T14704 arg1Of paraffin,for
R10232 T14705 T14704 arg2Of examination,for
R10233 T14706 T14707 arg1Of Sections,(
R10234 T14706 T14712 arg1Of Sections,were
R10235 T14706 T14713 arg2Of Sections,stained
R10236 T14706 T14719 arg2Of Sections,analyzed
R10237 T14709 T14707 arg2Of µm,(
R10238 T14709 T14708 arg1Of µm,4
R10239 T14709 T14710 arg1Of µm,thick
R10240 T14711 T14707 arg3Of ),(
R10241 T14713 T14714 arg1Of stained,with
R10242 T14713 T14718 arg1Of stained,and
R10243 T14717 T14714 arg2Of E,with
R10244 T14717 T14715 arg1Of E,H
R10245 T14717 T14716 arg1Of E,&
R10246 T14718 T14712 arg2Of and,were
R10247 T14719 T14718 arg2Of analyzed,and
R10248 T14721 T14719 arg1Of immunohistochemistry,analyzed
R10249 T14721 T14720 arg2Of immunohistochemistry,by
R10250 T14724 T14722 arg1Of sections,The
R10251 T14724 T14723 arg1Of sections,paraffin-embedded
R10252 T14724 T14725 arg1Of sections,were
R10253 T14724 T14726 arg2Of sections,deparaffinized
R10254 T14724 T14728 arg2Of sections,rehydrated
R10255 T14724 T14730 arg2Of sections,washed
R10256 T14724 T14737 arg2Of sections,subjected
R10257 T14726 T14727 arg1Of deparaffinized,and
R10258 T14727 T14729 arg1Of and,","
R10259 T14728 T14727 arg2Of rehydrated,and
R10260 T14729 T14735 arg1Of ",",and
R10261 T14730 T14729 arg2Of washed,","
R10262 T14730 T14731 arg1Of washed,in
R10263 T14733 T14731 arg2Of water,in
R10264 T14733 T14732 arg1Of water,distilled
R10265 T14735 T14725 arg2Of and,were
R10266 T14735 T14734 arg1Of and,","
R10267 T14737 T14735 arg2Of subjected,and
R10268 T14737 T14736 arg1Of subjected,then
R10269 T14737 T14738 arg1Of subjected,to
R10270 T14741 T14738 arg2Of retrieval,to
R10271 T14741 T14739 arg1Of retrieval,heat-mediated
R10272 T14741 T14740 arg1Of retrieval,antigen
R10273 T14744 T14742 arg1Of activity,Endogenous
R10274 T14744 T14743 arg1Of activity,peroxidase
R10275 T14744 T14745 arg1Of activity,was
R10276 T14744 T14746 arg2Of activity,quenched
R10277 T14746 T14745 arg2Of quenched,was
R10278 T14746 T14759 arg1Of quenched,","
R10279 T14746 T14760 modOf quenched,followed
R10280 T14748 T14746 arg1Of incubation,quenched
R10281 T14748 T14747 arg2Of incubation,by
R10282 T14748 T14749 arg1Of incubation,in
R10283 T14753 T14749 arg2Of peroxide,in
R10284 T14753 T14750 arg1Of peroxide,3
R10285 T14753 T14751 arg1Of peroxide,%
R10286 T14753 T14752 arg1Of peroxide,hydrogen
R10287 T14753 T14754 arg1Of peroxide,in
R10288 T14753 T14756 arg1Of peroxide,for
R10289 T14755 T14754 arg2Of methanol,in
R10290 T14758 T14756 arg2Of min,for
R10291 T14758 T14757 arg1Of min,15
R10292 T14760 T14761 arg1Of followed,by
R10293 T14762 T14761 arg2Of clearing,by
R10294 T14762 T14763 arg1Of clearing,in
R10295 T14762 T14765 arg1Of clearing,for
R10296 T14764 T14763 arg2Of PBS,in
R10297 T14767 T14765 arg2Of min,for
R10298 T14767 T14766 arg1Of min,5
R10299 T14769 T14768 arg1Of sections,The
R10300 T14769 T14770 arg1Of sections,were
R10301 T14769 T14771 arg2Of sections,blocked
R10302 T14769 T14788 arg2Of sections,incubated
R10303 T14771 T14772 arg1Of blocked,for
R10304 T14771 T14787 arg1Of blocked,and
R10305 T14774 T14772 arg2Of min,for
R10306 T14774 T14773 arg1Of min,30
R10307 T14774 T14775 arg1Of min,with
R10308 T14776 T14777 arg1Of 3,%
R10309 T14780 T14775 arg2Of serum,with
R10310 T14780 T14776 arg1Of serum,3
R10311 T14780 T14778 arg1Of serum,normal
R10312 T14780 T14779 arg1Of serum,horse
R10313 T14780 T14781 arg2Of serum,diluted
R10314 T14781 T14782 arg1Of diluted,in
R10315 T14783 T14782 arg2Of PBS,in
R10316 T14783 T14784 arg1Of PBS,","
R10317 T14783 T14785 arg2Of PBS,blotted
R10318 T14787 T14770 arg2Of and,were
R10319 T14787 T14786 arg1Of and,","
R10320 T14788 T14787 arg2Of incubated,and
R10321 T14788 T14789 arg1Of incubated,with
R10322 T14791 T14792 arg2Of mouse,anti-mouse
R10323 T14796 T14792 arg3Of antigen,anti-mouse
R10324 T14796 T14793 arg1Of antigen,proliferating
R10325 T14796 T14794 arg1Of antigen,cell
R10326 T14796 T14795 arg1Of antigen,nuclear
R10327 T14796 T14797 arg1Of antigen,(
R10328 T14798 T14797 arg2Of PCNA,(
R10329 T14798 T14799 arg1Of PCNA,","
R10330 T14801 T14799 arg2Of dilution,","
R10331 T14801 T14800 arg1Of dilution,1∶200
R10332 T14802 T14797 arg3Of ),(
R10333 T14804 T14789 arg2Of antibodies,with
R10334 T14804 T14790 arg1Of antibodies,primary
R10335 T14804 T14791 arg1Of antibodies,mouse
R10336 T14804 T14803 arg1Of antibodies,monoclonal
R10337 T14804 T14805 arg1Of antibodies,in
R10338 T14806 T14805 arg2Of blocking,in
R10339 T14806 T14808 arg1Of blocking,for
R10340 T14806 T14811 arg1Of blocking,at
R10341 T14807 T14806 arg2Of serum,blocking
R10342 T14810 T14808 arg2Of h,for
R10343 T14810 T14809 arg1Of h,4
R10344 T14813 T14811 arg2Of temperature,at
R10345 T14813 T14812 arg1Of temperature,room
R10346 T14817 T14816 arg1Of slides,the
R10347 T14817 T14818 arg1Of slides,were
R10348 T14817 T14819 arg2Of slides,washed
R10349 T14817 T14829 arg2Of slides,incubated
R10350 T14819 T14821 arg1Of washed,times
R10351 T14819 T14822 arg1Of washed,for
R10352 T14819 T14828 arg1Of washed,and
R10353 T14821 T14820 arg1Of times,three
R10354 T14824 T14822 arg2Of min,for
R10355 T14824 T14823 arg1Of min,5
R10356 T14824 T14825 arg1Of min,each
R10357 T14824 T14826 arg1Of min,in
R10358 T14827 T14826 arg2Of PBS,in
R10359 T14828 T14814 arg1Of and,Thereafter
R10360 T14828 T14815 arg1Of and,","
R10361 T14828 T14818 arg2Of and,were
R10362 T14829 T14828 arg2Of incubated,and
R10363 T14829 T14830 arg1Of incubated,with
R10364 T14832 T14833 arg1Of anti-mouse,and
R10365 T14834 T14833 arg2Of anti-rabbit,and
R10366 T14835 T14830 arg2Of antibodies,with
R10367 T14835 T14831 arg2Of antibodies,biotinylated
R10368 T14835 T14832 arg1Of antibodies,anti-mouse
R10369 T14835 T14834 arg1Of antibodies,anti-rabbit
R10370 T14835 T14836 arg1Of antibodies,for
R10371 T14838 T14836 arg2Of h,for
R10372 T14838 T14837 arg1Of h,2
R10373 T14840 T14839 arg1Of slides,The
R10374 T14840 T14841 arg1Of slides,were
R10375 T14840 T14842 arg2Of slides,washed
R10376 T14842 T14841 arg2Of washed,were
R10377 T14842 T14843 arg1Of washed,in
R10378 T14844 T14843 arg2Of PBS,in
R10379 T14844 T14845 arg1Of PBS,","
R10380 T14844 T14846 arg2Of PBS,followed
R10381 T14844 T14856 arg1Of PBS,(
R10382 T14849 T14846 arg1Of addition,followed
R10383 T14849 T14847 arg2Of addition,by
R10384 T14849 T14848 arg1Of addition,the
R10385 T14849 T14850 arg1Of addition,of
R10386 T14855 T14850 arg2Of complex,of
R10387 T14855 T14851 arg1Of complex,the
R10388 T14855 T14852 arg1Of complex,avidin
R10389 T14855 T14853 arg1Of complex,biotin
R10390 T14855 T14854 arg1Of complex,peroxidase
R10391 T14858 T14857 arg1Of kit,ABC
R10392 T14858 T14859 arg1Of kit,;
R10393 T14859 T14856 arg2Of ;,(
R10394 T14859 T14866 arg1Of ;,","
R10395 T14859 T14867 arg1Of ;,USA
R10396 T14861 T14860 arg1Of Laboratories,Vector
R10397 T14861 T14862 arg1Of Laboratories,","
R10398 T14862 T14864 arg1Of ",",","
R10399 T14863 T14862 arg2Of Burlingame,","
R10400 T14864 T14859 arg2Of ",",;
R10401 T14865 T14864 arg2Of CA,","
R10402 T14868 T14856 arg3Of ),(
R10403 T14870 T14869 arg1Of slides,The
R10404 T14870 T14871 arg1Of slides,were
R10405 T14870 T14872 arg2Of slides,washed
R10406 T14872 T14871 arg2Of washed,were
R10407 T14872 T14873 arg1Of washed,and
R10408 T14876 T14874 arg1Of reaction,the
R10409 T14876 T14875 arg1Of reaction,peroxidase
R10410 T14876 T14877 arg1Of reaction,was
R10411 T14876 T14878 arg2Of reaction,developed
R10412 T14878 T14873 arg2Of developed,and
R10413 T14878 T14877 arg2Of developed,was
R10414 T14878 T14879 arg1Of developed,with
R10415 T14878 T14886 arg1Of developed,","
R10416 T14878 T14887 modOf developed,followed
R10417 T14880 T14881 arg1Of diaminobenzidine,(
R10418 T14880 T14884 arg1Of diaminobenzidine,and
R10419 T14882 T14881 arg2Of DAB,(
R10420 T14883 T14881 arg3Of ),(
R10421 T14884 T14879 arg2Of and,with
R10422 T14885 T14884 arg2Of peroxide,and
R10423 T14889 T14890 arg1Of counterstaining,with
R10424 T14889 T14892 arg1Of counterstaining,","
R10425 T14889 T14893 modOf counterstaining,mounting
R10426 T14889 T14897 arg1Of counterstaining,and
R10427 T14891 T14890 arg2Of hematoxylin,with
R10428 T14893 T14894 arg1Of mounting,in
R10429 T14895 T14894 arg2Of aqua-mount,in
R10430 T14897 T14887 arg1Of and,followed
R10431 T14897 T14888 arg2Of and,by
R10432 T14897 T14896 arg1Of and,","
R10433 T14897 T14899 arg1Of and,under
R10434 T14898 T14897 arg2Of evaluation,and
R10435 T14902 T14899 arg2Of microscope,under
R10436 T14902 T14900 arg1Of microscope,a
R10437 T14902 T14901 arg1Of microscope,light
R10438 T14902 T14903 arg1Of microscope,(
R10439 T14904 T14903 arg2Of magnification×200,(
R10440 T14904 T14905 arg1Of magnification×200,;
R10441 T14906 T14905 arg2Of Olympus,;
R10442 T14906 T14907 arg1Of Olympus,","
R10443 T14908 T14907 arg2Of Tokyo,","
R10444 T14908 T14909 arg1Of Tokyo,","
R10445 T14910 T14909 arg2Of Japan,","
R10446 T14911 T14903 arg3Of ),(
R10447 T14914 T14912 arg1Of control,A
R10448 T14914 T14913 arg1Of control,negative
R10449 T14914 T14915 arg1Of control,was
R10450 T14914 T14916 arg2Of control,included
R10451 T14914 T14921 arg1Of control,omitting
R10452 T14916 T14915 arg2Of included,was
R10453 T14916 T14917 arg1Of included,in
R10454 T14916 T14920 arg1Of included,by
R10455 T14919 T14917 arg2Of experiments,in
R10456 T14919 T14918 arg1Of experiments,all
R10457 T14921 T14920 arg2Of omitting,by
R10458 T14924 T14921 arg2Of antibody,omitting
R10459 T14924 T14922 arg1Of antibody,the
R10460 T14924 T14923 arg1Of antibody,primary
R10461 T14927 T14925 arg2Of detection,For
R10462 T14927 T14926 arg1Of detection,the
R10463 T14927 T14928 arg1Of detection,of
R10464 T14927 T14932 arg1Of detection,in
R10465 T14931 T14928 arg2Of death,of
R10466 T14931 T14929 arg1Of death,apoptotic
R10467 T14931 T14930 arg1Of death,cell
R10468 T14935 T14932 arg2Of tissues,in
R10469 T14935 T14933 arg1Of tissues,the
R10470 T14935 T14934 arg1Of tissues,tumor
R10471 T14939 T14937 arg1Of sections,the
R10472 T14939 T14938 arg1Of sections,paraffin-embedded
R10473 T14939 T14940 arg1Of sections,were
R10474 T14939 T14941 arg2Of sections,incubated
R10475 T14939 T14968 arg1Of sections,washed
R10476 T14941 T14940 arg2Of incubated,were
R10477 T14941 T14942 arg1Of incubated,in
R10478 T14941 T14966 arg1Of incubated,and
R10479 T14944 T14945 arg1Of mixture,of
R10480 T14944 T14953 arg1Of mixture,and
R10481 T14948 T14945 arg2Of solution,of
R10482 T14948 T14946 arg1Of solution,the
R10483 T14948 T14947 arg1Of solution,labeling
R10484 T14948 T14949 arg1Of solution,(
R10485 T14951 T14949 arg2Of µl,(
R10486 T14951 T14950 arg1Of µl,540
R10487 T14952 T14949 arg3Of ),(
R10488 T14953 T14942 arg2Of and,in
R10489 T14953 T14943 arg1Of and,a
R10490 T14955 T14953 arg2Of solution,and
R10491 T14955 T14954 arg1Of solution,enzyme
R10492 T14955 T14956 arg1Of solution,(
R10493 T14955 T14960 arg1Of solution,for
R10494 T14958 T14956 arg2Of µl,(
R10495 T14958 T14957 arg1Of µl,60
R10496 T14959 T14956 arg3Of ),(
R10497 T14962 T14960 arg2Of h,for
R10498 T14962 T14961 arg1Of h,1
R10499 T14962 T14963 arg1Of h,at
R10500 T14964 T14963 arg2Of 37°C,at
R10501 T14966 T14925 arg1Of and,For
R10502 T14966 T14936 arg1Of and,","
R10503 T14966 T14965 arg1Of and,","
R10504 T14968 T14966 arg2Of washed,and
R10505 T14968 T14967 arg1Of washed,then
R10506 T14968 T14970 arg1Of washed,times
R10507 T14968 T14971 arg1Of washed,in
R10508 T14968 T14975 arg1Of washed,for
R10509 T14968 T14979 arg1Of washed,","
R10510 T14968 T14980 arg1Of washed,according
R10511 T14970 T14969 arg1Of times,three
R10512 T14972 T14973 arg1Of 0.1,M
R10513 T14974 T14971 arg2Of PBS,in
R10514 T14974 T14972 arg1Of PBS,0.1
R10515 T14977 T14975 arg2Of min,for
R10516 T14977 T14976 arg1Of min,5
R10517 T14978 T14968 arg2Of each,washed
R10518 T14981 T14980 arg2Of to,according
R10519 T14984 T14981 arg2Of instructions,to
R10520 T14984 T14982 arg1Of instructions,the
R10521 T14984 T14983 arg1Of instructions,manufacturer’s
R10522 T14988 T14987 arg1Of sections,the
R10523 T14988 T14989 arg1Of sections,were
R10524 T14988 T14990 arg2Of sections,incubated
R10525 T14988 T15010 arg2Of sections,mounted
R10526 T14988 T15015 arg1Of sections,cover-slipped
R10527 T14990 T14989 arg2Of incubated,were
R10528 T14990 T14991 arg1Of incubated,with
R10529 T14990 T14993 arg1Of incubated,","
R10530 T14992 T14991 arg2Of 4′,with
R10531 T14993 T14985 arg1Of ",",Next
R10532 T14993 T14986 arg1Of ",",","
R10533 T14993 T15010 modOf ",",mounted
R10534 T14993 T15015 modOf ",",cover-slipped
R10535 T14994 T14995 arg1Of 6-diamidino-2-phenylindole,(
R10536 T14994 T14998 arg1Of 6-diamidino-2-phenylindole,for
R10537 T14994 T15001 arg1Of 6-diamidino-2-phenylindole,at
R10538 T14994 T15007 arg1Of 6-diamidino-2-phenylindole,were
R10539 T14994 T15008 arg2Of 6-diamidino-2-phenylindole,rinsed
R10540 T14996 T14995 arg2Of DAPI,(
R10541 T14997 T14995 arg3Of ),(
R10542 T15000 T14998 arg2Of min,for
R10543 T15000 T14999 arg1Of min,15
R10544 T15003 T15001 arg2Of Finally,at
R10545 T15003 T15002 arg1Of Finally,37°C.
R10546 T15003 T15004 arg1Of Finally,","
R10547 T15006 T15004 arg2Of sections,","
R10548 T15006 T15005 arg1Of sections,the
R10549 T15008 T14993 arg2Of rinsed,","
R10550 T15008 T15007 arg2Of rinsed,were
R10551 T15008 T15009 arg1Of rinsed,","
R10552 T15010 T15011 arg1Of mounted,on
R10553 T15010 T15014 arg1Of mounted,and
R10554 T15012 T15011 arg2Of slides,on
R10555 T15014 T15013 arg1Of and,","
R10556 T15015 T15014 arg2Of cover-slipped,and
R10557 T15015 T15016 arg1Of cover-slipped,for
R10558 T15018 T15016 arg2Of microscopy,for
R10559 T15018 T15017 arg1Of microscopy,fluorescence
R10560 T15018 T15019 arg1Of microscopy,(
R10561 T15021 T15019 arg2Of microscope,(
R10562 T15021 T15020 arg1Of microscope,DAS
R10563 T15022 T15019 arg3Of ),(

UBERON-AE

Id Subject Object Predicate Lexical cue
T14659 1413-1420 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T14658 680-685 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T14657 513-518 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T14678 1394-1399 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T14677 1389-1399 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T14676 1379-1399 http://purl.obolibrary.org/obo/GO_0006915 denotes apoptotic cell death
T14675 1366-1393 http://purl.obolibrary.org/obo/GO_0043654 denotes detection of apoptotic cell
T14674 1002-1004 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14673 1043-1062 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase reaction
T14672 321-340 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase activity

GO-MF

Id Subject Object Predicate Lexical cue
T14692 1002-1004 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14691 1348-1356 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T14690 843-853 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14689 657-667 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14688 1043-1062 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase reaction
T14687 321-340 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase activity

GO-CC

Id Subject Object Predicate Lexical cue
T14686 1348-1356 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T14685 843-853 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14684 657-667 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14683 1348-1356 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T14682 843-853 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14681 657-667 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14680 1389-1393 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T14679 602-606 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T14671 1663-1876 Sentence denotes Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope).
T14670 1358-1662 Sentence denotes For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions.
T14669 1273-1357 Sentence denotes A negative control was included in all experiments by omitting the primary antibody.
T14668 1012-1272 Sentence denotes The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan).
T14667 863-1011 Sentence denotes The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA).
T14666 715-862 Sentence denotes Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h.
T14665 455-714 Sentence denotes The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature.
T14664 310-454 Sentence denotes Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min.
T14663 161-309 Sentence denotes The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval.
T14662 79-160 Sentence denotes Sections (4 µm thick) were stained with H&E and analyzed by immunohistochemistry.
T14661 0-78 Sentence denotes All specimens were fixed in formalin and embedded in paraffin for examination.
T227 0-78 Sentence denotes All specimens were fixed in formalin and embedded in paraffin for examination.
T228 79-160 Sentence denotes Sections (4 µm thick) were stained with H&E and analyzed by immunohistochemistry.
T229 161-309 Sentence denotes The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval.
T230 310-454 Sentence denotes Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min.
T231 455-714 Sentence denotes The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature.
T232 715-862 Sentence denotes Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h.
T233 863-1011 Sentence denotes The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA).
T234 1012-1272 Sentence denotes The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan).
T235 1273-1357 Sentence denotes A negative control was included in all experiments by omitting the primary antibody.
T236 1358-1662 Sentence denotes For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions.
T237 1663-1758 Sentence denotes Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C.
T238 1759-1876 Sentence denotes Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope).

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T15412 1273-1356 Positive_regulation denotes A negative control was included in all experiments by omitting the primary antibody
T15411 259-308 Binding denotes then subjected to heat-mediated antigen retrieval
T15410 1519-1542 Entity denotes enzyme solution (60 µl)
T15409 28-36 Entity denotes formalin
T15408 636-644 Entity denotes dilution
T15407 919-958 Protein denotes of the avidin biotin peroxidase complex
T15406 607-629 Protein denotes nuclear antigen (PCNA,
T15405 646-667 Protein denotes monoclonal antibodies
T15404 919-958 Entity denotes of the avidin biotin peroxidase complex
T15403 1109-1118 Entity denotes peroxide,
T15402 1717-1738 Entity denotes 2-phenylindole (DAPI)
T15401 165-182 Entity denotes paraffin-embedded
T15400 690-693 Entity denotes 4 h
T15399 1519-1525 Protein denotes enzyme
T15398 291-298 Entity denotes antigen
T15397 1336-1356 Entity denotes the primary antibody
T15396 919-958 Entity denotes of the avidin biotin peroxidase complex
T15395 1152-1163 Entity denotes hematoxylin
T15394 1043-1053 Protein denotes peroxidase
T15393 1547-1559 Entity denotes 1 h at 37°C,
T15392 1733-1737 Entity denotes DAPI
T15391 53-61 Entity denotes paraffin
T15390 1471-1542 Entity denotes a mixture of the labeling solution (540 µl) and enzyme solution (60 µl)
T15389 1426-1443 Entity denotes paraffin-embedded
T15388 1860-1863 Entity denotes DAS
T15387 843-853 Entity denotes antibodies
T15386 321-331 Protein denotes peroxidase
T15385 602-635 Entity denotes cell nuclear antigen (PCNA, 1∶200
T15384 1484-1514 Entity denotes the labeling solution (540 µl)
T15383 940-950 Protein denotes peroxidase
T15382 624-628 Protein denotes PCNA
T15381 395-403 Entity denotes methanol
T15380 1177-1181 Entity denotes aqua
T15379 860-861 Entity denotes h
T15378 371-403 Entity denotes 3% hydrogen peroxide in methanol

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T15351 1743-1745 CD denotes 15
T15377 1875-1876 . denotes .
T15376 1874-1875 -RRB- denotes )
T15375 1864-1874 NN denotes microscope
T15374 1860-1863 NNP denotes DAS
T15373 1859-1860 -LRB- denotes (
T15372 1848-1858 NN denotes microscopy
T15371 1835-1847 NN denotes fluorescence
T15370 1831-1834 IN denotes for
T15369 1817-1830 JJ denotes cover-slipped
T15368 1813-1816 CC denotes and
T15367 1811-1812 , denotes ,
T15366 1805-1811 NNS denotes slides
T15365 1802-1804 IN denotes on
T15364 1794-1801 VBN denotes mounted
T15363 1792-1793 , denotes ,
T15362 1786-1792 VBN denotes rinsed
T15361 1781-1785 VBD denotes were
T15360 1772-1780 NNS denotes sections
T15359 1768-1771 DT denotes the
T15358 1766-1767 , denotes ,
T15357 1759-1766 RB denotes Finally
T15356 1756-1758 NNP denotes C.
T15355 1755-1756 CD denotes °
T15354 1753-1755 CD denotes 37
T15353 1750-1752 IN denotes at
T15352 1746-1749 NN denotes min
T15350 1739-1742 IN denotes for
T15349 1737-1738 -RRB- denotes )
T15348 1733-1737 NNP denotes DAPI
T15347 1732-1733 -LRB- denotes (
T15346 1707-1731 NN denotes diamidino-2-phenylindole
T15345 1706-1707 : denotes -
T15344 1704-1706 SYM denotes ,6
T15343 1703-1704 CD denotes ′
T15342 1702-1703 CD denotes 4
T15341 1697-1701 IN denotes with
T15340 1687-1696 VBN denotes incubated
T15339 1682-1686 VBD denotes were
T15338 1673-1681 NNS denotes sections
T15337 1669-1672 DT denotes the
T15336 1667-1668 , denotes ,
T15335 1663-1667 JJ denotes Next
T15334 1661-1662 . denotes .
T15333 1649-1661 NNS denotes instructions
T15332 1634-1646 NN denotes manufacturer
T15331 1630-1633 DT denotes the
T15330 1627-1629 TO denotes to
T15329 1617-1626 VBG denotes according
T15328 1615-1616 , denotes ,
T15327 1611-1615 DT denotes each
T15326 1607-1610 NN denotes min
T15325 1605-1606 CD denotes 5
T15324 1601-1604 IN denotes for
T15323 1597-1600 NNP denotes PBS
T15322 1595-1596 NNP denotes M
T15321 1591-1594 CD denotes 0.1
T15320 1588-1590 IN denotes in
T15319 1582-1587 NNS denotes times
T15318 1576-1581 CD denotes three
T15317 1569-1575 VBD denotes washed
T15316 1564-1568 RB denotes then
T15315 1560-1563 CC denotes and
T15314 1558-1559 , denotes ,
T15313 1557-1558 NNP denotes C
T15312 1556-1557 CD denotes °
T15311 1554-1556 CD denotes 37
T15310 1551-1553 IN denotes at
T15309 1549-1550 NN denotes h
T15308 1547-1548 CD denotes 1
T15307 1543-1546 IN denotes for
T15306 1541-1542 -RRB- denotes )
T15305 1539-1541 NN denotes µl
T15304 1536-1538 CD denotes 60
T15303 1535-1536 -LRB- denotes (
T15302 1526-1534 NN denotes solution
T15301 1519-1525 VB denotes enzyme
T15300 1515-1518 CC denotes and
T15299 1513-1514 -RRB- denotes )
T15298 1511-1513 NN denotes µl
T15297 1507-1510 CD denotes 540
T15296 1506-1507 -LRB- denotes (
T15295 1497-1505 NN denotes solution
T15294 1488-1496 VBG denotes labeling
T15293 1484-1487 DT denotes the
T15292 1481-1483 IN denotes of
T15291 1473-1480 NN denotes mixture
T15290 1471-1472 DT denotes a
T15289 1468-1470 IN denotes in
T15288 1458-1467 VBN denotes incubated
T15287 1453-1457 VBD denotes were
T15286 1444-1452 NNS denotes sections
T15285 1426-1443 JJ denotes paraffin-embedded
T15284 1422-1425 DT denotes the
T15283 1420-1421 , denotes ,
T15282 1413-1420 NNS denotes tissues
T15281 1407-1412 NN denotes tumor
T15280 1403-1406 DT denotes the
T15279 1400-1402 IN denotes in
T15278 1394-1399 NN denotes death
T15277 1389-1393 NN denotes cell
T15276 1379-1388 JJ denotes apoptotic
T15275 1376-1378 IN denotes of
T15274 1366-1375 NN denotes detection
T15273 1362-1365 DT denotes the
T15272 1358-1361 IN denotes For
T15271 1356-1357 . denotes .
T15270 1348-1356 NN denotes antibody
T15269 1340-1347 JJ denotes primary
T15268 1336-1339 DT denotes the
T15267 1327-1335 VBG denotes omitting
T15266 1324-1326 IN denotes by
T15265 1312-1323 NNS denotes experiments
T15264 1308-1311 DT denotes all
T15263 1305-1307 IN denotes in
T15262 1296-1304 VBN denotes included
T15261 1292-1295 VBD denotes was
T15260 1284-1291 NN denotes control
T15259 1275-1283 JJ denotes negative
T15258 1273-1274 DT denotes A
T15257 1271-1272 . denotes .
T15256 1270-1271 -RRB- denotes )
T15255 1265-1270 NNP denotes Japan
T15254 1263-1264 , denotes ,
T15253 1258-1263 NNP denotes Tokyo
T15252 1256-1257 , denotes ,
T15251 1249-1256 NNP denotes Olympus
T15250 1247-1248 : denotes ;
T15249 1244-1247 CD denotes 200
T15248 1243-1244 RB denotes ×
T15247 1230-1243 NN denotes magnification
T15117 507-512 NN denotes horse
T15116 500-506 JJ denotes normal
T15115 498-499 NN denotes %
T15114 497-498 CD denotes 3
T15113 492-496 IN denotes with
T15112 488-491 NN denotes min
T15111 485-487 CD denotes 30
T15110 481-484 IN denotes for
T15109 473-480 VBN denotes blocked
T15108 468-472 VBD denotes were
T15107 459-467 NNS denotes sections
T15106 455-458 DT denotes The
T15105 453-454 . denotes .
T15104 450-453 NN denotes min
T15103 448-449 CD denotes 5
T15102 444-447 IN denotes for
T15101 440-443 NNP denotes PBS
T15100 437-439 IN denotes in
T15099 428-436 VBG denotes clearing
T15098 425-427 IN denotes by
T15097 416-424 VBN denotes followed
T15096 414-415 , denotes ,
T15095 411-414 NN denotes min
T15094 408-410 CD denotes 15
T15093 404-407 IN denotes for
T15092 395-403 NN denotes methanol
T15091 392-394 IN denotes in
T15090 383-391 NN denotes peroxide
T15089 374-382 NN denotes hydrogen
T15088 372-373 NN denotes %
T15087 371-372 CD denotes 3
T15086 368-370 IN denotes in
T15085 357-367 NN denotes incubation
T15084 354-356 IN denotes by
T15083 345-353 VBN denotes quenched
T15082 341-344 VBD denotes was
T15081 332-340 NN denotes activity
T15080 321-331 NN denotes peroxidase
T15079 310-320 JJ denotes Endogenous
T15078 308-309 . denotes .
T15077 299-308 NN denotes retrieval
T15076 291-298 NN denotes antigen
T15075 277-290 JJ denotes heat-mediated
T15074 274-276 TO denotes to
T15073 264-273 VBN denotes subjected
T15072 259-263 RB denotes then
T15071 255-258 CC denotes and
T15070 253-254 , denotes ,
T15069 248-253 NN denotes water
T15068 238-247 JJ denotes distilled
T15067 235-237 IN denotes in
T15066 228-234 VBN denotes washed
T15065 226-227 , denotes ,
T15064 216-226 VBN denotes rehydrated
T15063 212-215 CC denotes and
T15062 197-211 VBN denotes deparaffinized
T15061 192-196 VBD denotes were
T15060 183-191 NNS denotes sections
T15059 165-182 JJ denotes paraffin-embedded
T15058 161-164 DT denotes The
T15057 159-160 . denotes .
T15056 139-159 NN denotes immunohistochemistry
T15055 136-138 IN denotes by
T15054 127-135 VBN denotes analyzed
T15053 123-126 CC denotes and
T15052 119-122 NNP denotes H&E
T15051 114-118 IN denotes with
T15050 106-113 VBN denotes stained
T15049 101-105 VBD denotes were
T15048 99-100 -RRB- denotes )
T15047 94-99 JJ denotes thick
T15046 91-93 NN denotes µm
T15045 89-90 CD denotes 4
T15044 88-89 -LRB- denotes (
T15043 79-87 NNS denotes Sections
T15042 77-78 . denotes .
T15041 66-77 NN denotes examination
T15040 62-65 IN denotes for
T15039 53-61 NN denotes paraffin
T15038 50-52 IN denotes in
T15037 41-49 VBN denotes embedded
T15036 37-40 CC denotes and
T15035 28-36 NN denotes formalin
T15034 25-27 IN denotes in
T15033 19-24 VBN denotes fixed
T15032 14-18 VBD denotes were
T15031 4-13 NNS denotes specimens
T15030 0-3 NNP denotes All
T15246 1229-1230 -LRB- denotes (
T15245 1218-1228 NN denotes microscope
T15244 1212-1217 JJ denotes light
T15243 1210-1211 DT denotes a
T15242 1204-1209 IN denotes under
T15241 1193-1203 NN denotes evaluation
T15240 1189-1192 CC denotes and
T15239 1187-1188 , denotes ,
T15238 1177-1187 JJ denotes aqua-mount
T15237 1174-1176 IN denotes in
T15236 1165-1173 VBG denotes mounting
T15235 1163-1164 , denotes ,
T15234 1152-1163 NN denotes hematoxylin
T15233 1147-1151 IN denotes with
T15232 1131-1146 VBG denotes counterstaining
T15231 1128-1130 IN denotes by
T15230 1119-1127 VBN denotes followed
T15229 1117-1118 , denotes ,
T15228 1109-1117 NN denotes peroxide
T15227 1105-1108 CC denotes and
T15226 1103-1104 -RRB- denotes )
T15225 1100-1103 NNP denotes DAB
T15224 1099-1100 -LRB- denotes (
T15199 960-963 NNP denotes ABC
T15198 959-960 -LRB- denotes (
T15197 951-958 NN denotes complex
T15196 940-950 NN denotes peroxidase
T15195 933-939 NN denotes biotin
T15194 926-932 NN denotes avidin
T15193 922-925 DT denotes the
T15192 919-921 IN denotes of
T15191 910-918 NN denotes addition
T15190 906-909 DT denotes the
T15189 903-905 IN denotes by
T15188 894-902 VBN denotes followed
T15187 892-893 , denotes ,
T15186 889-892 NNP denotes PBS
T15185 886-888 IN denotes in
T15184 879-885 VBN denotes washed
T15183 874-878 VBD denotes were
T15182 867-873 NNS denotes slides
T15181 863-866 DT denotes The
T15180 861-862 . denotes .
T15179 860-861 NN denotes h
T15178 858-859 CD denotes 2
T15177 854-857 IN denotes for
T15176 843-853 NNS denotes antibodies
T15175 831-842 JJ denotes anti-rabbit
T15174 827-830 CC denotes and
T15173 816-826 NN denotes anti-mouse
T15172 803-815 JJ denotes biotinylated
T15171 798-802 IN denotes with
T15170 788-797 VBN denotes incubated
T15169 784-787 CC denotes and
T15168 780-783 NNP denotes PBS
T15167 777-779 IN denotes in
T15166 772-776 DT denotes each
T15165 768-771 NN denotes min
T15164 766-767 CD denotes 5
T15163 762-765 IN denotes for
T15162 756-761 NNS denotes times
T15161 750-755 CD denotes three
T15160 743-749 VBN denotes washed
T15159 738-742 VBD denotes were
T15158 731-737 NNS denotes slides
T15157 727-730 DT denotes the
T15156 725-726 , denotes ,
T15155 715-725 RB denotes Thereafter
T15154 713-714 . denotes .
T15153 702-713 NN denotes temperature
T15152 697-701 NN denotes room
T15151 694-696 IN denotes at
T15150 692-693 NN denotes h
T15149 690-691 CD denotes 4
T15148 686-689 IN denotes for
T15147 680-685 NN denotes serum
T15146 671-679 VBG denotes blocking
T15145 668-670 IN denotes in
T15144 657-667 NNS denotes antibodies
T15143 646-656 JJ denotes monoclonal
T15142 644-645 -RRB- denotes )
T15141 636-644 NN denotes dilution
T15140 632-635 CD denotes 200
T15139 631-632 CD denotes ∶
T15138 630-631 CD denotes 1
T15137 628-629 , denotes ,
T15136 624-628 NNP denotes PCNA
T15135 623-624 -LRB- denotes (
T15134 615-622 NN denotes antigen
T15133 607-614 JJ denotes nuclear
T15132 602-606 NN denotes cell
T15131 588-601 VBG denotes proliferating
T15130 577-587 JJ denotes anti-mouse
T15129 571-576 NN denotes mouse
T15128 563-570 JJ denotes primary
T15127 558-562 IN denotes with
T15125 544-547 CC denotes and
T15124 542-543 , denotes ,
T15123 535-542 VBD denotes blotted
T15122 533-534 , denotes ,
T15121 530-533 NNP denotes PBS
T15120 527-529 IN denotes in
T15119 519-526 VBN denotes diluted
T15118 513-518 NN denotes serum
T15223 1082-1098 NN denotes diaminobenzidine
T15222 1077-1081 IN denotes with
T15221 1067-1076 VBN denotes developed
T15220 1063-1066 VBD denotes was
T15219 1054-1062 NN denotes reaction
T15218 1043-1053 JJ denotes peroxidase
T15217 1039-1042 DT denotes the
T15216 1035-1038 CC denotes and
T15215 1028-1034 VBN denotes washed
T15214 1023-1027 VBD denotes were
T15213 1016-1022 NNS denotes slides
T15212 1012-1015 DT denotes The
T15211 1010-1011 . denotes .
T15210 1009-1010 -RRB- denotes )
T15209 1006-1009 NNP denotes USA
T15208 1004-1005 , denotes ,
T15207 1002-1004 NNP denotes CA
T15206 1000-1001 , denotes ,
T15205 990-1000 NNP denotes Burlingame
T15204 988-989 , denotes ,
T15203 976-988 NNPS denotes Laboratories
T15202 969-975 NNP denotes Vector
T15201 967-968 : denotes ;
T15200 964-967 NN denotes kit
T15126 548-557 VBN denotes incubated
R10850 T15315 T15288 cc and,incubated
R10851 T15316 T15317 advmod then,washed
R10565 T15030 T15031 det All,specimens
R10566 T15031 T15033 nsubjpass specimens,fixed
R10567 T15032 T15033 auxpass were,fixed
R10568 T15033 T15033 ROOT fixed,fixed
R10569 T15034 T15033 prep in,fixed
R10570 T15035 T15034 pobj formalin,in
R10571 T15036 T15033 cc and,fixed
R10572 T15037 T15033 conj embedded,fixed
R10573 T15038 T15037 prep in,embedded
R10574 T15039 T15038 pobj paraffin,in
R10575 T15040 T15037 prep for,embedded
R10576 T15041 T15040 pobj examination,for
R10577 T15042 T15033 punct .,fixed
R10578 T15043 T15050 nsubjpass Sections,stained
R10579 T15044 T15043 punct (,Sections
R10580 T15045 T15046 nummod 4,µm
R10581 T15046 T15047 npadvmod µm,thick
R10582 T15047 T15043 appos thick,Sections
R10583 T15048 T15043 punct ),Sections
R10584 T15049 T15050 auxpass were,stained
R10585 T15050 T15050 ROOT stained,stained
R10586 T15051 T15050 prep with,stained
R10587 T15052 T15051 pobj H&E,with
R10588 T15053 T15050 cc and,stained
R10589 T15054 T15050 conj analyzed,stained
R10590 T15055 T15054 agent by,analyzed
R10591 T15056 T15055 pobj immunohistochemistry,by
R10592 T15057 T15050 punct .,stained
R10593 T15058 T15060 det The,sections
R10594 T15059 T15060 amod paraffin-embedded,sections
R10595 T15060 T15062 nsubjpass sections,deparaffinized
R10596 T15061 T15062 auxpass were,deparaffinized
R10597 T15062 T15062 ROOT deparaffinized,deparaffinized
R10598 T15063 T15062 cc and,deparaffinized
R10599 T15064 T15062 conj rehydrated,deparaffinized
R10600 T15065 T15064 punct ",",rehydrated
R10601 T15066 T15064 conj washed,rehydrated
R10602 T15067 T15066 prep in,washed
R10603 T15068 T15069 amod distilled,water
R10604 T15069 T15067 pobj water,in
R10605 T15070 T15066 punct ",",washed
R10606 T15071 T15066 cc and,washed
R10607 T15072 T15073 advmod then,subjected
R10608 T15073 T15066 conj subjected,washed
R10609 T15074 T15073 prep to,subjected
R10610 T15075 T15077 amod heat-mediated,retrieval
R10611 T15076 T15077 compound antigen,retrieval
R10612 T15077 T15074 pobj retrieval,to
R10613 T15078 T15062 punct .,deparaffinized
R10614 T15079 T15081 amod Endogenous,activity
R10615 T15080 T15081 compound peroxidase,activity
R10616 T15081 T15083 nsubjpass activity,quenched
R10617 T15082 T15083 auxpass was,quenched
R10618 T15083 T15083 ROOT quenched,quenched
R10619 T15084 T15083 agent by,quenched
R10620 T15085 T15084 pobj incubation,by
R10621 T15086 T15083 prep in,quenched
R10622 T15087 T15088 nummod 3,%
R10623 T15088 T15090 compound %,peroxide
R10624 T15089 T15090 compound hydrogen,peroxide
R10625 T15090 T15086 pobj peroxide,in
R10626 T15091 T15083 prep in,quenched
R10627 T15092 T15091 pobj methanol,in
R10628 T15093 T15083 prep for,quenched
R10629 T15094 T15095 nummod 15,min
R10630 T15095 T15093 pobj min,for
R10631 T15096 T15095 punct ",",min
R10632 T15097 T15083 advcl followed,quenched
R10633 T15098 T15097 prep by,followed
R10634 T15099 T15098 pcomp clearing,by
R10635 T15100 T15099 prep in,clearing
R10636 T15101 T15100 pobj PBS,in
R10637 T15102 T15099 prep for,clearing
R10638 T15103 T15104 nummod 5,min
R10639 T15104 T15102 pobj min,for
R10640 T15105 T15083 punct .,quenched
R10641 T15106 T15107 det The,sections
R10642 T15107 T15109 nsubjpass sections,blocked
R10643 T15108 T15109 auxpass were,blocked
R10644 T15109 T15109 ROOT blocked,blocked
R10645 T15110 T15109 prep for,blocked
R10646 T15111 T15112 nummod 30,min
R10647 T15112 T15110 pobj min,for
R10648 T15113 T15112 prep with,min
R10649 T15114 T15115 nummod 3,%
R10650 T15115 T15123 compound %,blotted
R10651 T15116 T15118 amod normal,serum
R10652 T15117 T15118 compound horse,serum
R10653 T15118 T15123 nsubj serum,blotted
R10654 T15119 T15118 acl diluted,serum
R10655 T15120 T15119 prep in,diluted
R10656 T15121 T15120 pobj PBS,in
R10657 T15122 T15118 punct ",",serum
R10658 T15123 T15109 advcl blotted,blocked
R10659 T15124 T15123 punct ",",blotted
R10660 T15125 T15123 cc and,blotted
R10661 T15126 T15123 conj incubated,blotted
R10662 T15127 T15126 prep with,incubated
R10663 T15128 T15129 amod primary,mouse
R10664 T15129 T15134 nmod mouse,antigen
R10665 T15130 T15134 amod anti-mouse,antigen
R10666 T15131 T15134 amod proliferating,antigen
R10667 T15132 T15134 compound cell,antigen
R10668 T15133 T15134 amod nuclear,antigen
R10669 T15134 T15127 pobj antigen,with
R10670 T15135 T15134 punct (,antigen
R10671 T15136 T15134 appos PCNA,antigen
R10672 T15137 T15136 punct ",",PCNA
R10673 T15138 T15140 nummod 1,200
R10674 T15139 T15140 nummod ∶,200
R10675 T15140 T15141 npadvmod 200,dilution
R10676 T15141 T15136 appos dilution,PCNA
R10677 T15142 T15136 punct ),PCNA
R10678 T15143 T15144 amod monoclonal,antibodies
R10679 T15144 T15134 appos antibodies,antigen
R10680 T15145 T15126 prep in,incubated
R10681 T15146 T15145 pcomp blocking,in
R10682 T15147 T15146 dobj serum,blocking
R10683 T15148 T15146 prep for,blocking
R10684 T15149 T15150 nummod 4,h
R10685 T15150 T15148 pobj h,for
R10686 T15151 T15150 prep at,h
R10687 T15152 T15153 compound room,temperature
R10688 T15153 T15151 pobj temperature,at
R10689 T15154 T15109 punct .,blocked
R10690 T15155 T15160 advmod Thereafter,washed
R10691 T15156 T15160 punct ",",washed
R10692 T15157 T15158 det the,slides
R10693 T15158 T15160 nsubjpass slides,washed
R10694 T15159 T15160 auxpass were,washed
R10695 T15160 T15160 ROOT washed,washed
R10696 T15161 T15162 nummod three,times
R10697 T15162 T15160 npadvmod times,washed
R10698 T15163 T15160 prep for,washed
R10699 T15164 T15165 nummod 5,min
R10700 T15165 T15163 pobj min,for
R10701 T15166 T15170 nsubj each,incubated
R10702 T15167 T15166 prep in,each
R10703 T15168 T15167 pobj PBS,in
R10704 T15169 T15166 cc and,each
R10705 T15170 T15160 advcl incubated,washed
R10706 T15171 T15170 prep with,incubated
R10707 T15172 T15173 amod biotinylated,anti-mouse
R10708 T15173 T15171 pobj anti-mouse,with
R10709 T15174 T15173 cc and,anti-mouse
R10710 T15175 T15176 amod anti-rabbit,antibodies
R10711 T15176 T15173 conj antibodies,anti-mouse
R10712 T15177 T15170 prep for,incubated
R10713 T15178 T15179 nummod 2,h
R10714 T15179 T15177 pobj h,for
R10715 T15180 T15160 punct .,washed
R10716 T15181 T15182 det The,slides
R10717 T15182 T15184 nsubjpass slides,washed
R10718 T15183 T15184 auxpass were,washed
R10719 T15184 T15203 ccomp washed,Laboratories
R10720 T15185 T15184 prep in,washed
R10721 T15186 T15185 pobj PBS,in
R10722 T15187 T15184 punct ",",washed
R10723 T15188 T15184 advcl followed,washed
R10724 T15189 T15188 agent by,followed
R10725 T15190 T15191 det the,addition
R10726 T15191 T15189 pobj addition,by
R10727 T15192 T15191 prep of,addition
R10728 T15193 T15197 det the,complex
R10729 T15194 T15197 nmod avidin,complex
R10730 T15195 T15197 nmod biotin,complex
R10731 T15196 T15197 compound peroxidase,complex
R10732 T15197 T15200 amod complex,kit
R10733 T15198 T15200 punct (,kit
R10734 T15199 T15200 compound ABC,kit
R10735 T15200 T15192 pobj kit,of
R10736 T15201 T15203 punct ;,Laboratories
R10737 T15202 T15203 compound Vector,Laboratories
R10738 T15203 T15203 ROOT Laboratories,Laboratories
R10739 T15204 T15203 punct ",",Laboratories
R10740 T15205 T15203 conj Burlingame,Laboratories
R10741 T15206 T15205 punct ",",Burlingame
R10742 T15207 T15205 conj CA,Burlingame
R10743 T15208 T15207 punct ",",CA
R10744 T15209 T15207 conj USA,CA
R10745 T15210 T15205 punct ),Burlingame
R10746 T15211 T15203 punct .,Laboratories
R10747 T15212 T15213 det The,slides
R10748 T15213 T15215 nsubjpass slides,washed
R10749 T15214 T15215 auxpass were,washed
R10750 T15215 T15215 ROOT washed,washed
R10751 T15216 T15215 cc and,washed
R10752 T15217 T15219 det the,reaction
R10753 T15218 T15219 amod peroxidase,reaction
R10754 T15219 T15221 nsubjpass reaction,developed
R10755 T15220 T15221 auxpass was,developed
R10756 T15221 T15215 conj developed,washed
R10757 T15222 T15221 prep with,developed
R10758 T15223 T15222 pobj diaminobenzidine,with
R10759 T15224 T15225 punct (,DAB
R10760 T15225 T15223 appos DAB,diaminobenzidine
R10761 T15226 T15225 punct ),DAB
R10762 T15227 T15223 cc and,diaminobenzidine
R10763 T15228 T15223 conj peroxide,diaminobenzidine
R10764 T15229 T15221 punct ",",developed
R10765 T15230 T15221 advcl followed,developed
R10766 T15231 T15221 agent by,developed
R10767 T15232 T15231 pcomp counterstaining,by
R10768 T15233 T15232 prep with,counterstaining
R10769 T15234 T15233 pobj hematoxylin,with
R10770 T15235 T15232 punct ",",counterstaining
R10771 T15236 T15232 conj mounting,counterstaining
R10772 T15237 T15236 prep in,mounting
R10773 T15238 T15237 pobj aqua-mount,in
R10774 T15239 T15236 punct ",",mounting
R10775 T15240 T15236 cc and,mounting
R10776 T15241 T15236 conj evaluation,mounting
R10777 T15242 T15241 prep under,evaluation
R10778 T15243 T15245 det a,microscope
R10779 T15244 T15245 amod light,microscope
R10780 T15245 T15242 pobj microscope,under
R10781 T15246 T15248 punct (,×
R10782 T15247 T15248 nsubj magnification,×
R10783 T15248 T15241 parataxis ×,evaluation
R10784 T15249 T15248 npadvmod 200,×
R10785 T15250 T15248 punct ;,×
R10786 T15251 T15248 conj Olympus,×
R10787 T15252 T15251 punct ",",Olympus
R10788 T15253 T15251 conj Tokyo,Olympus
R10789 T15254 T15253 punct ",",Tokyo
R10790 T15255 T15253 conj Japan,Tokyo
R10791 T15256 T15253 punct ),Tokyo
R10792 T15257 T15221 punct .,developed
R10793 T15258 T15260 det A,control
R10794 T15259 T15260 amod negative,control
R10795 T15260 T15262 nsubjpass control,included
R10796 T15261 T15262 auxpass was,included
R10797 T15262 T15262 ROOT included,included
R10798 T15263 T15262 prep in,included
R10799 T15264 T15265 det all,experiments
R10800 T15265 T15263 pobj experiments,in
R10801 T15266 T15262 prep by,included
R10802 T15267 T15266 pcomp omitting,by
R10803 T15268 T15270 det the,antibody
R10804 T15269 T15270 amod primary,antibody
R10805 T15270 T15267 dobj antibody,omitting
R10806 T15271 T15262 punct .,included
R10807 T15272 T15288 prep For,incubated
R10808 T15273 T15274 det the,detection
R10809 T15274 T15272 pobj detection,For
R10810 T15275 T15274 prep of,detection
R10811 T15276 T15278 amod apoptotic,death
R10812 T15277 T15278 compound cell,death
R10813 T15278 T15275 pobj death,of
R10814 T15279 T15274 prep in,detection
R10815 T15280 T15282 det the,tissues
R10816 T15281 T15282 compound tumor,tissues
R10817 T15282 T15279 pobj tissues,in
R10818 T15283 T15288 punct ",",incubated
R10819 T15284 T15286 det the,sections
R10820 T15285 T15286 amod paraffin-embedded,sections
R10821 T15286 T15288 nsubjpass sections,incubated
R10822 T15287 T15288 auxpass were,incubated
R10823 T15288 T15288 ROOT incubated,incubated
R10824 T15289 T15288 prep in,incubated
R10825 T15290 T15291 det a,mixture
R10826 T15291 T15289 pobj mixture,in
R10827 T15292 T15291 prep of,mixture
R10828 T15293 T15295 det the,solution
R10829 T15294 T15295 amod labeling,solution
R10830 T15295 T15292 pobj solution,of
R10831 T15296 T15295 punct (,solution
R10832 T15297 T15298 nummod 540,µl
R10833 T15298 T15295 appos µl,solution
R10834 T15299 T15295 punct ),solution
R10835 T15300 T15288 cc and,incubated
R10836 T15301 T15302 compound enzyme,solution
R10837 T15302 T15288 conj solution,incubated
R10838 T15303 T15305 punct (,µl
R10839 T15304 T15305 nummod 60,µl
R10840 T15305 T15302 appos µl,solution
R10841 T15306 T15302 punct ),solution
R10842 T15307 T15302 prep for,solution
R10843 T15308 T15309 nummod 1,h
R10844 T15309 T15307 pobj h,for
R10845 T15310 T15302 prep at,solution
R10846 T15311 T15312 nummod 37,°
R10847 T15312 T15310 pobj °,at
R10848 T15313 T15302 npadvmod C,solution
R10849 T15314 T15302 punct ",",solution
R10852 T15317 T15288 conj washed,incubated
R10853 T15318 T15319 nummod three,times
R10854 T15319 T15317 npadvmod times,washed
R10855 T15320 T15317 prep in,washed
R10856 T15321 T15323 nummod 0.1,PBS
R10857 T15322 T15323 compound M,PBS
R10858 T15323 T15320 pobj PBS,in
R10859 T15324 T15317 prep for,washed
R10860 T15325 T15326 nummod 5,min
R10861 T15326 T15324 pobj min,for
R10862 T15327 T15326 appos each,min
R10863 T15328 T15317 punct ",",washed
R10864 T15329 T15317 prep according,washed
R10865 T15330 T15329 prep to,according
R10866 T15331 T15332 det the,manufacturer
R10867 T15332 T15333 poss manufacturer,instructions
R10868 T15333 T15330 pobj instructions,to
R10869 T15334 T15288 punct .,incubated
R10870 T15335 T15340 advmod Next,incubated
R10871 T15336 T15340 punct ",",incubated
R10872 T15337 T15338 det the,sections
R10873 T15338 T15340 nsubjpass sections,incubated
R10874 T15339 T15340 auxpass were,incubated
R10875 T15340 T15340 ROOT incubated,incubated
R10876 T15341 T15340 prep with,incubated
R10877 T15342 T15343 nummod 4,′
R10878 T15343 T15341 pobj ′,with
R10879 T15344 T15346 punct ",6",diamidino-2-phenylindole
R10880 T15345 T15346 punct -,diamidino-2-phenylindole
R10881 T15346 T15346 ROOT diamidino-2-phenylindole,diamidino-2-phenylindole
R10882 T15347 T15348 punct (,DAPI
R10883 T15348 T15346 appos DAPI,diamidino-2-phenylindole
R10884 T15349 T15346 punct ),diamidino-2-phenylindole
R10885 T15350 T15346 prep for,diamidino-2-phenylindole
R10886 T15351 T15352 nummod 15,min
R10887 T15352 T15350 pobj min,for
R10888 T15353 T15346 prep at,diamidino-2-phenylindole
R10889 T15354 T15355 nummod 37,°
R10890 T15355 T15353 pobj °,at
R10891 T15356 T15353 pobj C.,at
R10892 T15357 T15362 advmod Finally,rinsed
R10893 T15358 T15362 punct ",",rinsed
R10894 T15359 T15360 det the,sections
R10895 T15360 T15362 nsubjpass sections,rinsed
R10896 T15361 T15362 auxpass were,rinsed
R10897 T15362 T15362 ROOT rinsed,rinsed
R10898 T15363 T15362 punct ",",rinsed
R10899 T15364 T15362 conj mounted,rinsed
R10900 T15365 T15364 prep on,mounted
R10901 T15366 T15365 pobj slides,on
R10902 T15367 T15365 punct ",",on
R10903 T15368 T15364 cc and,mounted
R10904 T15369 T15364 conj cover-slipped,mounted
R10905 T15370 T15369 prep for,cover-slipped
R10906 T15371 T15372 compound fluorescence,microscopy
R10907 T15372 T15370 pobj microscopy,for
R10908 T15373 T15375 punct (,microscope
R10909 T15374 T15375 compound DAS,microscope
R10910 T15375 T15372 appos microscope,microscopy
R10911 T15376 T15372 punct ),microscopy
R10912 T15377 T15362 punct .,rinsed

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T15025 1043-1053 Protein denotes peroxidase
T15024 926-950 Protein denotes avidin biotin peroxidase
T15023 310-331 Protein denotes Endogenous peroxidase