PMC:3342329 / 29830-31727 JSON TXT

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pmc-enju-pas

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T14659","span":{"begin":1434,"end":1441},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T14658","span":{"begin":701,"end":706},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T14657","span":{"begin":534,"end":539},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"Immunohistochemistry\nAll specimens were fixed in formalin and embedded in paraffin for examination. Sections (4 µm thick) were stained with H\u0026E and analyzed by immunohistochemistry. The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval. Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min. The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature. Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h. The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA). The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan). A negative control was included in all experiments by omitting the primary antibody. For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions. Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope)."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T14678","span":{"begin":1415,"end":1420},"obj":"http://purl.obolibrary.org/obo/GO_0016265"},{"id":"T14677","span":{"begin":1410,"end":1420},"obj":"http://purl.obolibrary.org/obo/GO_0008219"},{"id":"T14676","span":{"begin":1400,"end":1420},"obj":"http://purl.obolibrary.org/obo/GO_0006915"},{"id":"T14675","span":{"begin":1387,"end":1414},"obj":"http://purl.obolibrary.org/obo/GO_0043654"},{"id":"T14674","span":{"begin":1023,"end":1025},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T14673","span":{"begin":1064,"end":1083},"obj":"http://purl.obolibrary.org/obo/GO_0004601"},{"id":"T14672","span":{"begin":342,"end":361},"obj":"http://purl.obolibrary.org/obo/GO_0004601"}],"text":"Immunohistochemistry\nAll specimens were fixed in formalin and embedded in paraffin for examination. Sections (4 µm thick) were stained with H\u0026E and analyzed by immunohistochemistry. The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval. Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min. The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature. Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h. The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA). The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan). A negative control was included in all experiments by omitting the primary antibody. For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions. Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope)."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T14692","span":{"begin":1023,"end":1025},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T14691","span":{"begin":1369,"end":1377},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T14690","span":{"begin":864,"end":874},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T14689","span":{"begin":678,"end":688},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T14688","span":{"begin":1064,"end":1083},"obj":"http://purl.obolibrary.org/obo/GO_0004601"},{"id":"T14687","span":{"begin":342,"end":361},"obj":"http://purl.obolibrary.org/obo/GO_0004601"}],"text":"Immunohistochemistry\nAll specimens were fixed in formalin and embedded in paraffin for examination. Sections (4 µm thick) were stained with H\u0026E and analyzed by immunohistochemistry. The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval. Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min. The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature. Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h. The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA). The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan). A negative control was included in all experiments by omitting the primary antibody. For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions. Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope)."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T14686","span":{"begin":1369,"end":1377},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T14685","span":{"begin":864,"end":874},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T14684","span":{"begin":678,"end":688},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T14683","span":{"begin":1369,"end":1377},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T14682","span":{"begin":864,"end":874},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T14681","span":{"begin":678,"end":688},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T14680","span":{"begin":1410,"end":1414},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14679","span":{"begin":623,"end":627},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Immunohistochemistry\nAll specimens were fixed in formalin and embedded in paraffin for examination. Sections (4 µm thick) were stained with H\u0026E and analyzed by immunohistochemistry. The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval. Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min. The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature. Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h. The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA). The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan). A negative control was included in all experiments by omitting the primary antibody. For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions. Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope)."}

sentences

{"project":"sentences","denotations":[{"id":"T14671","span":{"begin":1684,"end":1897},"obj":"Sentence"},{"id":"T14670","span":{"begin":1379,"end":1683},"obj":"Sentence"},{"id":"T14669","span":{"begin":1294,"end":1378},"obj":"Sentence"},{"id":"T14668","span":{"begin":1033,"end":1293},"obj":"Sentence"},{"id":"T14667","span":{"begin":884,"end":1032},"obj":"Sentence"},{"id":"T14666","span":{"begin":736,"end":883},"obj":"Sentence"},{"id":"T14665","span":{"begin":476,"end":735},"obj":"Sentence"},{"id":"T14664","span":{"begin":331,"end":475},"obj":"Sentence"},{"id":"T14663","span":{"begin":182,"end":330},"obj":"Sentence"},{"id":"T14662","span":{"begin":100,"end":181},"obj":"Sentence"},{"id":"T14661","span":{"begin":21,"end":99},"obj":"Sentence"},{"id":"T14660","span":{"begin":0,"end":20},"obj":"Sentence"},{"id":"T226","span":{"begin":0,"end":20},"obj":"Sentence"},{"id":"T227","span":{"begin":21,"end":99},"obj":"Sentence"},{"id":"T228","span":{"begin":100,"end":181},"obj":"Sentence"},{"id":"T229","span":{"begin":182,"end":330},"obj":"Sentence"},{"id":"T230","span":{"begin":331,"end":475},"obj":"Sentence"},{"id":"T231","span":{"begin":476,"end":735},"obj":"Sentence"},{"id":"T232","span":{"begin":736,"end":883},"obj":"Sentence"},{"id":"T233","span":{"begin":884,"end":1032},"obj":"Sentence"},{"id":"T234","span":{"begin":1033,"end":1293},"obj":"Sentence"},{"id":"T235","span":{"begin":1294,"end":1378},"obj":"Sentence"},{"id":"T236","span":{"begin":1379,"end":1683},"obj":"Sentence"},{"id":"T237","span":{"begin":1684,"end":1779},"obj":"Sentence"},{"id":"T238","span":{"begin":1780,"end":1897},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Immunohistochemistry\nAll specimens were fixed in formalin and embedded in paraffin for examination. Sections (4 µm thick) were stained with H\u0026E and analyzed by immunohistochemistry. The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval. Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min. The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature. Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h. The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA). The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan). A negative control was included in all experiments by omitting the primary antibody. For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions. Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope)."}

bionlp-st-ge-2016-test-ihmc

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T15025","span":{"begin":1064,"end":1074},"obj":"Protein"},{"id":"T15024","span":{"begin":947,"end":971},"obj":"Protein"},{"id":"T15023","span":{"begin":331,"end":352},"obj":"Protein"}],"text":"Immunohistochemistry\nAll specimens were fixed in formalin and embedded in paraffin for examination. Sections (4 µm thick) were stained with H\u0026E and analyzed by immunohistochemistry. The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval. Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min. The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature. Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h. The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA). The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan). A negative control was included in all experiments by omitting the primary antibody. For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions. Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope)."}

PMC:3342329 / 29830-31727 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:3342329 / 29830-31727 JSON TXT