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PMC:3333881 / 6562-8079 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
22187158-14532125-77584438 108-110 14532125 denotes 52
22187158-19782003-77584439 325-327 19782003 denotes 53

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4915 0-5 NN denotes Cells
T4916 5-6 -COMMA- denotes ,
T4917 7-19 NN denotes transfection
T4918 19-20 -COMMA- denotes ,
T4919 21-31 NN denotes treatments
T4920 32-35 CC denotes and
T4921 36-46 NN denotes luciferase
T4922 47-52 NN denotes assay
T4923 53-57 NN denotes HeLa
T4924 57-58 -COMMA- denotes ,
T4925 59-71 NN denotes p50−/−p65−/−
T4926 72-77 NN denotes mouse
T4927 78-87 JJ denotes embryonic
T4928 88-99 NN denotes fibroblasts
T4929 100-101 -LRB- denotes (
T4930 101-105 NN denotes MEFs
T4931 105-106 -RRB- denotes )
T4932 107-108 -LRB- denotes (
T4933 108-110 CD denotes 52
T4934 110-111 -RRB- denotes )
T4935 112-115 CC denotes and
T4936 116-120 NN denotes 293T
T4937 121-126 NN denotes cells
T4938 127-131 VB denotes were
T4939 132-140 VB denotes cultured
T4940 141-143 IN denotes in
T4941 144-152 NNP denotes Dulbecco
T4942 152-154 POS denotes 's
T4943 155-163 VB denotes modified
T4944 164-169 NNP denotes Eagle
T4945 169-171 POS denotes 's
T4946 172-178 NN denotes medium
T4947 178-179 -COLON- denotes ;
T4948 180-186 NN denotes Jurkat
T4949 186-187 -COMMA- denotes ,
T4950 188-192 NN denotes U937
T4951 193-198 NN denotes cells
T4952 199-202 CC denotes and
T4953 203-208 JJ denotes human
T4954 209-219 JJ denotes peripheral
T4955 220-225 NN denotes blood
T4956 226-237 JJ denotes mononuclear
T4957 238-243 NN denotes cells
T4958 244-245 -LRB- denotes (
T4959 245-250 NN denotes PBMCs
T4960 250-251 -RRB- denotes )
T4961 252-256 VB denotes were
T4962 257-265 VB denotes cultured
T4963 266-268 IN denotes in
T4964 269-273 NN denotes RPMI
T4965 274-278 CD denotes 1640
T4966 280-285 NN denotes PBMCs
T4967 286-290 VB denotes were
T4968 291-299 VB denotes isolated
T4969 300-302 IN denotes as
T4970 303-313 RB denotes previously
T4971 314-323 VB denotes described
T4972 324-325 -LRB- denotes (
T4973 325-327 CD denotes 53
T4974 327-328 -RRB- denotes )
T4975 330-335 NN denotes Media
T4976 336-340 VB denotes were
T4977 341-353 VB denotes supplemented
T4978 354-358 IN denotes with
T4979 359-361 CD denotes 10
T4980 361-362 NN denotes %
T4981 363-379 JJ denotes heat-inactivated
T4982 380-385 JJ denotes fetal
T4983 386-390 NN denotes calf
T4984 391-396 NN denotes serum
T4985 397-400 CC denotes and
T4986 401-405 NN denotes 2 mM
T4987 406-417 NN denotes l-glutamine
T4988 418-419 -LRB- denotes (
T4989 419-424 NNP denotes Lonza
T4990 425-432 NNP denotes Cologne
T4991 433-435 NNP denotes AG
T4992 435-436 -COMMA- denotes ,
T4993 437-444 NNP denotes Germany
T4994 444-445 -RRB- denotes )
T4995 447-451 NN denotes HeLa
T4996 451-452 -COMMA- denotes ,
T4997 453-465 NN denotes p50−/−p65−/−
T4998 466-470 NN denotes MEFs
T4999 471-474 CC denotes and
T5000 475-479 NN denotes 293T
T5001 480-484 VB denotes were
T5002 485-496 VB denotes transfected
T5003 497-501 IN denotes with
T5004 502-505 NN denotes DNA
T5005 506-508 IN denotes by
T5006 509-514 VB denotes using
T5007 515-521 NN denotes FuGENE
T5008 522-524 NN denotes HD
T5009 525-526 -LRB- denotes (
T5010 526-531 NNP denotes Roche
T5011 532-542 NNP denotes Diagnostic
T5012 543-547 NNP denotes GmbH
T5013 547-548 -COMMA- denotes ,
T5014 549-557 NNP denotes Mannheim
T5015 557-558 -COMMA- denotes ,
T5016 559-566 NNP denotes Germany
T5017 566-567 -RRB- denotes )
T5018 567-568 -COMMA- denotes ,
T5019 569-578 VB denotes according
T5020 579-581 TO denotes to
T5021 582-585 DT denotes the
T5022 586-598 NN denotes manufacturer
T5023 598-600 POS denotes 's
T5024 601-609 NN denotes protocol
T5025 609-610 -COLON- denotes ;
T5026 611-616 JJ denotes total
T5027 617-620 NN denotes DNA
T5028 621-628 NN denotes amounts
T5029 629-633 VB denotes were
T5030 634-643 VB denotes equalized
T5031 644-646 IN denotes by
T5032 647-659 NN denotes transfection
T5033 660-662 IN denotes of
T5034 663-670 NN denotes pRc/CMV
T5035 671-676 NN denotes empty
T5036 677-683 NN denotes vector
T5037 684-685 -LRB- denotes (
T5038 685-695 NN denotes Invitrogen
T5039 695-696 -COMMA- denotes ,
T5040 697-705 NNP denotes Carlsbad
T5041 705-706 -COMMA- denotes ,
T5042 707-709 NNP denotes CA
T5043 709-710 -COMMA- denotes ,
T5044 711-714 NNP denotes USA
T5045 714-715 -RRB- denotes )
T5046 717-720 IN denotes For
T5047 721-738 NN denotes pulse-stimulation
T5048 738-739 -COMMA- denotes ,
T5049 740-744 NN denotes HeLa
T5050 745-750 NN denotes cells
T5051 751-755 VB denotes were
T5052 756-763 VB denotes treated
T5053 764-768 IN denotes with
T5054 769-776 NN denotes phorbol
T5055 777-789 NN denotes 12-myristate
T5056 790-800 NN denotes 13-acetate
T5057 801-802 -LRB- denotes (
T5058 802-805 NN denotes PMA
T5059 805-806 -COLON- denotes ;
T5060 807-820 JJ denotes Sigma-Aldrich
T5061 820-821 -COMMA- denotes ,
T5062 822-824 NNP denotes St
T5063 825-830 NNP denotes Louis
T5064 830-831 -COMMA- denotes ,
T5065 832-834 NN denotes MO
T5066 834-835 -COMMA- denotes ,
T5067 836-839 NNP denotes USA
T5068 839-840 -RRB- denotes )
T5069 841-842 -LRB- denotes (
T5070 842-850 NN denotes 20 ng/ml
T5071 850-851 -RRB- denotes )
T5072 852-855 IN denotes for
T5073 856-861 NN denotes 5 min
T5074 861-862 -COMMA- denotes ,
T5075 863-865 CC denotes or
T5076 866-871 NN denotes tumor
T5077 872-880 NN denotes necrosis
T5078 881-889 NN denotes factor-α
T5079 890-891 -LRB- denotes (
T5080 891-896 NN denotes TNF-α
T5081 896-897 -COLON- denotes ;
T5082 898-911 JJ denotes Sigma-Aldrich
T5083 911-912 -RRB- denotes )
T5084 913-914 -LRB- denotes (
T5085 914-922 NN denotes 20 ng/ml
T5086 922-923 -RRB- denotes )
T5087 924-927 IN denotes for
T5088 928-934 NN denotes 30 min
T5089 934-935 -COMMA- denotes ,
T5090 936-942 VB denotes washed
T5091 943-948 RB denotes twice
T5092 949-951 IN denotes in
T5093 952-960 JJ denotes complete
T5094 961-968 NN denotes culture
T5095 969-975 NN denotes medium
T5096 976-979 CC denotes and
T5097 980-984 RB denotes then
T5098 985-993 VB denotes returned
T5099 994-996 TO denotes to
T5100 997-1004 NN denotes culture
T5101 1006-1009 IN denotes For
T5102 1010-1020 NN denotes luciferase
T5103 1021-1027 NN denotes assays
T5104 1027-1028 -COMMA- denotes ,
T5105 1029-1038 NN denotes pSV-β-Gal
T5106 1039-1042 VB denotes was
T5107 1043-1057 VB denotes co-transfected
T5108 1058-1062 IN denotes with
T5109 1063-1069 NN denotes pκBluc
T5110 1070-1072 TO denotes to
T5111 1073-1080 VB denotes monitor
T5112 1081-1084 DT denotes the
T5113 1085-1097 NN denotes transfection
T5114 1098-1108 NN denotes efficiency
T5115 1110-1126 JJ denotes Forty-eight-hour
T5116 1127-1144 JJ denotes post-transfection
T5117 1144-1145 -COMMA- denotes ,
T5118 1146-1151 NN denotes cells
T5119 1152-1156 VB denotes were
T5120 1157-1162 VB denotes lysed
T5121 1163-1165 IN denotes in
T5122 1166-1171 NN denotes lysis
T5123 1172-1178 NN denotes buffer
T5124 1179-1181 IN denotes of
T5125 1182-1186 NNP denotes Dual
T5126 1187-1192 NNP denotes Light
T5127 1193-1203 NNP denotes Luciferase
T5128 1204-1210 NNP denotes System
T5129 1211-1212 -LRB- denotes (
T5130 1212-1218 NNP denotes Tropix
T5131 1218-1219 -COMMA- denotes ,
T5132 1220-1227 NNP denotes Bedford
T5133 1227-1228 -COMMA- denotes ,
T5134 1229-1231 NNP denotes MA
T5135 1231-1232 -COMMA- denotes ,
T5136 1233-1236 NNP denotes USA
T5137 1236-1237 -RRB- denotes )
T5138 1238-1241 CC denotes and
T5139 1242-1245 DT denotes the
T5140 1246-1256 NN denotes luciferase
T5141 1257-1260 CC denotes and
T5142 1261-1276 NN denotes β-galactosidase
T5143 1277-1287 NN denotes activities
T5144 1288-1292 VB denotes were
T5145 1293-1302 VB denotes evaluated
T5146 1303-1305 IN denotes by
T5147 1306-1311 VB denotes using
T5148 1312-1316 NNP denotes Dual
T5149 1317-1322 NNP denotes Light
T5150 1323-1333 NNP denotes Luciferase
T5151 1334-1340 NNP denotes System
T5152 1341-1342 -LRB- denotes (
T5153 1342-1348 NN denotes Tropix
T5154 1348-1349 -RRB- denotes )
T5155 1350-1352 IN denotes in
T5156 1353-1354 DT denotes a
T5157 1355-1369 NN denotes bioluminometer
T5158 1370-1371 -LRB- denotes (
T5159 1371-1377 NNP denotes Turner
T5160 1378-1387 NNP denotes Biosystem
T5161 1387-1388 -COMMA- denotes ,
T5162 1389-1398 NNP denotes Sunnyvale
T5163 1398-1399 -COMMA- denotes ,
T5164 1400-1402 NNP denotes CA
T5165 1402-1403 -COMMA- denotes ,
T5166 1404-1407 NNP denotes USA
T5167 1407-1408 -RRB- denotes )
T5168 1410-1413 DT denotes The
T5169 1414-1419 NN denotes ratio
T5170 1420-1422 IN denotes of
T5171 1423-1430 NN denotes firefly
T5172 1431-1441 NN denotes luciferase
T5173 1442-1450 NN denotes activity
T5174 1451-1453 TO denotes to
T5175 1454-1469 NN denotes β-galactosidase
T5176 1470-1478 NN denotes activity
T5177 1479-1482 VB denotes was
T5178 1483-1492 VB denotes expressed
T5179 1493-1495 IN denotes as
T5180 1496-1504 JJ denotes relative
T5181 1505-1510 JJ denotes light
T5182 1511-1516 NN denotes units
R4308 T4915 T4916 arg1Of Cells,","
R4309 T4916 T4918 arg1Of ",",","
R4310 T4917 T4916 arg2Of transfection,","
R4311 T4918 T4920 arg1Of ",",and
R4312 T4919 T4918 arg2Of treatments,","
R4313 T4922 T4920 arg2Of assay,and
R4314 T4922 T4921 arg1Of assay,luciferase
R4315 T4923 T4924 arg1Of HeLa,","
R4316 T4925 T4924 arg2Of p50−/−p65−/−,","
R4317 T4928 T4923 arg1Of fibroblasts,HeLa
R4318 T4928 T4925 arg1Of fibroblasts,p50−/−p65−/−
R4319 T4928 T4926 arg1Of fibroblasts,mouse
R4320 T4928 T4927 arg1Of fibroblasts,embryonic
R4321 T4928 T4929 arg1Of fibroblasts,(
R4322 T4928 T4932 arg1Of fibroblasts,(
R4323 T4928 T4935 arg1Of fibroblasts,and
R4324 T4930 T4929 arg2Of MEFs,(
R4325 T4931 T4929 arg3Of ),(
R4326 T4933 T4932 arg2Of 52,(
R4327 T4934 T4932 arg3Of ),(
R4328 T4935 T4938 arg1Of and,were
R4329 T4935 T4939 arg2Of and,cultured
R4330 T4937 T4935 arg2Of cells,and
R4331 T4937 T4936 arg1Of cells,293T
R4332 T4939 T4938 arg2Of cultured,were
R4333 T4939 T4940 arg1Of cultured,in
R4334 T4939 T4947 arg1Of cultured,;
R4335 T4941 T4942 arg2Of Dulbecco,'s
R4336 T4944 T4942 arg1Of Eagle,'s
R4337 T4944 T4943 arg2Of Eagle,modified
R4338 T4944 T4945 arg2Of Eagle,'s
R4339 T4946 T4940 arg2Of medium,in
R4340 T4946 T4945 arg1Of medium,'s
R4341 T4948 T4949 arg1Of Jurkat,","
R4342 T4949 T4952 arg1Of ",",and
R4343 T4951 T4949 arg2Of cells,","
R4344 T4951 T4950 arg1Of cells,U937
R4345 T4952 T4958 arg1Of and,(
R4346 T4952 T4961 arg1Of and,were
R4347 T4952 T4962 arg2Of and,cultured
R4348 T4957 T4952 arg2Of cells,and
R4349 T4957 T4953 arg1Of cells,human
R4350 T4957 T4954 arg1Of cells,peripheral
R4351 T4957 T4955 arg1Of cells,blood
R4352 T4957 T4956 arg1Of cells,mononuclear
R4353 T4959 T4958 arg2Of PBMCs,(
R4354 T4960 T4958 arg3Of ),(
R4355 T4962 T4938 modOf cultured,were
R4356 T4962 T4961 arg2Of cultured,were
R4357 T4962 T4963 arg1Of cultured,in
R4358 T4964 T4963 arg2Of RPMI,in
R4359 T4964 T4965 arg1Of RPMI,1640
R4360 T4966 T4967 arg1Of PBMCs,were
R4361 T4966 T4968 arg2Of PBMCs,isolated
R4362 T4968 T4967 arg2Of isolated,were
R4363 T4968 T4969 arg1Of isolated,as
R4364 T4971 T4969 arg2Of described,as
R4365 T4971 T4970 arg1Of described,previously
R4366 T4971 T4972 arg1Of described,(
R4367 T4973 T4972 arg2Of 53,(
R4368 T4974 T4972 arg3Of ),(
R4369 T4975 T4976 arg1Of Media,were
R4370 T4975 T4977 arg2Of Media,supplemented
R4371 T4977 T4976 arg2Of supplemented,were
R4372 T4977 T4978 arg1Of supplemented,with
R4373 T4979 T4980 arg1Of 10,%
R4374 T4984 T4981 arg1Of serum,heat-inactivated
R4375 T4984 T4982 arg1Of serum,fetal
R4376 T4984 T4983 arg1Of serum,calf
R4377 T4984 T4985 arg1Of serum,and
R4378 T4985 T4978 arg2Of and,with
R4379 T4985 T4979 arg1Of and,10
R4380 T4985 T4988 arg1Of and,(
R4381 T4987 T4985 arg2Of l-glutamine,and
R4382 T4987 T4986 arg1Of l-glutamine,2 mM
R4383 T4991 T4988 arg2Of AG,(
R4384 T4991 T4989 arg1Of AG,Lonza
R4385 T4991 T4990 arg1Of AG,Cologne
R4386 T4991 T4992 arg1Of AG,","
R4387 T4993 T4992 arg2Of Germany,","
R4388 T4994 T4988 arg3Of ),(
R4389 T4995 T4996 arg1Of HeLa,","
R4390 T4996 T4999 arg1Of ",",and
R4391 T4998 T4996 arg2Of MEFs,","
R4392 T4998 T4997 arg1Of MEFs,p50−/−p65−/−
R4393 T4999 T5001 arg1Of and,were
R4394 T4999 T5002 arg2Of and,transfected
R4395 T5000 T4999 arg2Of 293T,and
R4396 T5002 T5001 arg2Of transfected,were
R4397 T5002 T5003 arg1Of transfected,with
R4398 T5002 T5005 arg1Of transfected,by
R4399 T5002 T5018 arg1Of transfected,","
R4400 T5002 T5019 arg1Of transfected,according
R4401 T5002 T5025 arg1Of transfected,;
R4402 T5004 T5003 arg2Of DNA,with
R4403 T5006 T5005 arg2Of using,by
R4404 T5008 T5006 arg2Of HD,using
R4405 T5008 T5007 arg1Of HD,FuGENE
R4406 T5008 T5009 arg1Of HD,(
R4407 T5012 T5009 arg2Of GmbH,(
R4408 T5012 T5010 arg1Of GmbH,Roche
R4409 T5012 T5011 arg1Of GmbH,Diagnostic
R4410 T5012 T5013 arg1Of GmbH,","
R4411 T5014 T5013 arg2Of Mannheim,","
R4412 T5014 T5015 arg1Of Mannheim,","
R4413 T5016 T5015 arg2Of Germany,","
R4414 T5017 T5009 arg3Of ),(
R4415 T5020 T5019 arg2Of to,according
R4416 T5023 T5020 arg2Of 's,to
R4417 T5023 T5021 arg1Of 's,the
R4418 T5023 T5022 arg1Of 's,manufacturer
R4419 T5023 T5024 arg1Of 's,protocol
R4420 T5028 T5026 arg1Of amounts,total
R4421 T5028 T5027 arg1Of amounts,DNA
R4422 T5028 T5029 arg1Of amounts,were
R4423 T5028 T5030 arg2Of amounts,equalized
R4424 T5030 T5025 arg2Of equalized,;
R4425 T5030 T5029 arg2Of equalized,were
R4426 T5032 T5030 arg1Of transfection,equalized
R4427 T5032 T5031 arg2Of transfection,by
R4428 T5032 T5033 arg1Of transfection,of
R4429 T5036 T5033 arg2Of vector,of
R4430 T5036 T5034 arg1Of vector,pRc/CMV
R4431 T5036 T5035 arg1Of vector,empty
R4432 T5036 T5037 arg1Of vector,(
R4433 T5038 T5037 arg2Of Invitrogen,(
R4434 T5038 T5039 arg1Of Invitrogen,","
R4435 T5038 T5043 arg1Of Invitrogen,","
R4436 T5038 T5044 arg1Of Invitrogen,USA
R4437 T5040 T5041 arg1Of Carlsbad,","
R4438 T5041 T5039 arg2Of ",",","
R4439 T5042 T5041 arg2Of CA,","
R4440 T5045 T5037 arg3Of ),(
R4441 T5047 T5046 arg2Of pulse-stimulation,For
R4442 T5050 T5049 arg1Of cells,HeLa
R4443 T5050 T5051 arg1Of cells,were
R4444 T5050 T5052 arg2Of cells,treated
R4445 T5050 T5098 arg1Of cells,returned
R4446 T5052 T5051 arg2Of treated,were
R4447 T5052 T5053 arg1Of treated,with
R4448 T5052 T5096 arg1Of treated,and
R4449 T5056 T5054 arg1Of 13-acetate,phorbol
R4450 T5056 T5055 arg1Of 13-acetate,12-myristate
R4451 T5056 T5057 arg1Of 13-acetate,(
R4452 T5056 T5069 arg1Of 13-acetate,(
R4453 T5056 T5072 arg1Of 13-acetate,for
R4454 T5056 T5075 arg1Of 13-acetate,or
R4455 T5058 T5057 arg2Of PMA,(
R4456 T5058 T5059 arg1Of PMA,;
R4457 T5060 T5059 arg2Of Sigma-Aldrich,;
R4458 T5060 T5061 arg1Of Sigma-Aldrich,","
R4459 T5060 T5066 arg1Of Sigma-Aldrich,","
R4460 T5060 T5067 arg1Of Sigma-Aldrich,USA
R4461 T5063 T5061 arg2Of Louis,","
R4462 T5063 T5062 arg1Of Louis,St
R4463 T5063 T5064 arg1Of Louis,","
R4464 T5065 T5064 arg2Of MO,","
R4465 T5068 T5057 arg3Of ),(
R4466 T5070 T5069 arg2Of 20 ng/ml,(
R4467 T5071 T5069 arg3Of ),(
R4468 T5073 T5072 arg2Of 5 min,for
R4469 T5075 T5053 arg2Of or,with
R4470 T5075 T5074 arg1Of or,","
R4471 T5078 T5075 arg2Of factor-α,or
R4472 T5078 T5076 arg1Of factor-α,tumor
R4473 T5078 T5077 arg1Of factor-α,necrosis
R4474 T5078 T5079 arg1Of factor-α,(
R4475 T5078 T5084 arg1Of factor-α,(
R4476 T5078 T5087 arg1Of factor-α,for
R4477 T5078 T5089 arg1Of factor-α,","
R4478 T5078 T5090 arg2Of factor-α,washed
R4479 T5080 T5079 arg2Of TNF-α,(
R4480 T5080 T5081 arg1Of TNF-α,;
R4481 T5080 T5082 arg1Of TNF-α,Sigma-Aldrich
R4482 T5083 T5079 arg3Of ),(
R4483 T5085 T5084 arg2Of 20 ng/ml,(
R4484 T5086 T5084 arg3Of ),(
R4485 T5088 T5087 arg2Of 30 min,for
R4486 T5090 T5091 arg1Of washed,twice
R4487 T5090 T5092 arg1Of washed,in
R4488 T5095 T5092 arg2Of medium,in
R4489 T5095 T5093 arg1Of medium,complete
R4490 T5095 T5094 arg1Of medium,culture
R4491 T5096 T5046 arg1Of and,For
R4492 T5096 T5048 arg1Of and,","
R4493 T5098 T5096 arg2Of returned,and
R4494 T5098 T5097 arg1Of returned,then
R4495 T5098 T5099 arg1Of returned,to
R4496 T5100 T5099 arg2Of culture,to
R4497 T5103 T5101 arg2Of assays,For
R4498 T5103 T5102 arg1Of assays,luciferase
R4499 T5105 T5106 arg1Of pSV-β-Gal,was
R4500 T5105 T5107 arg2Of pSV-β-Gal,co-transfected
R4501 T5107 T5101 arg1Of co-transfected,For
R4502 T5107 T5104 arg1Of co-transfected,","
R4503 T5107 T5106 arg2Of co-transfected,was
R4504 T5107 T5108 arg1Of co-transfected,with
R4505 T5107 T5110 modOf co-transfected,to
R4506 T5109 T5108 arg2Of pκBluc,with
R4507 T5111 T5110 arg1Of monitor,to
R4508 T5114 T5111 arg2Of efficiency,monitor
R4509 T5114 T5112 arg1Of efficiency,the
R4510 T5114 T5113 arg1Of efficiency,transfection
R4511 T5118 T5115 arg1Of cells,Forty-eight-hour
R4512 T5118 T5116 arg1Of cells,post-transfection
R4513 T5118 T5117 arg1Of cells,","
R4514 T5118 T5119 arg1Of cells,were
R4515 T5118 T5120 arg2Of cells,lysed
R4516 T5120 T5119 arg2Of lysed,were
R4517 T5120 T5121 arg1Of lysed,in
R4518 T5120 T5138 arg1Of lysed,and
R4519 T5123 T5121 arg2Of buffer,in
R4520 T5123 T5122 arg1Of buffer,lysis
R4521 T5123 T5124 arg1Of buffer,of
R4522 T5128 T5124 arg2Of System,of
R4523 T5128 T5125 arg1Of System,Dual
R4524 T5128 T5126 arg1Of System,Light
R4525 T5128 T5127 arg1Of System,Luciferase
R4526 T5128 T5129 arg1Of System,(
R4527 T5130 T5131 arg1Of Tropix,","
R4528 T5131 T5133 arg1Of ",",","
R4529 T5132 T5131 arg2Of Bedford,","
R4530 T5133 T5129 arg2Of ",",(
R4531 T5133 T5135 arg1Of ",",","
R4532 T5134 T5133 arg2Of MA,","
R4533 T5136 T5135 arg2Of USA,","
R4534 T5137 T5129 arg3Of ),(
R4535 T5140 T5141 arg1Of luciferase,and
R4536 T5142 T5141 arg2Of β-galactosidase,and
R4537 T5143 T5139 arg1Of activities,the
R4538 T5143 T5140 arg1Of activities,luciferase
R4539 T5143 T5142 arg1Of activities,β-galactosidase
R4540 T5143 T5144 arg1Of activities,were
R4541 T5143 T5145 arg2Of activities,evaluated
R4542 T5145 T5138 arg2Of evaluated,and
R4543 T5145 T5144 arg2Of evaluated,were
R4544 T5145 T5146 arg1Of evaluated,by
R4545 T5147 T5146 arg2Of using,by
R4546 T5147 T5155 arg1Of using,in
R4547 T5147 T5158 arg1Of using,(
R4548 T5151 T5147 arg2Of System,using
R4549 T5151 T5148 arg1Of System,Dual
R4550 T5151 T5149 arg1Of System,Light
R4551 T5151 T5150 arg1Of System,Luciferase
R4552 T5151 T5152 arg1Of System,(
R4553 T5153 T5152 arg2Of Tropix,(
R4554 T5154 T5152 arg3Of ),(
R4555 T5157 T5155 arg2Of bioluminometer,in
R4556 T5157 T5156 arg1Of bioluminometer,a
R4557 T5160 T5158 arg2Of Biosystem,(
R4558 T5160 T5159 arg1Of Biosystem,Turner
R4559 T5160 T5161 arg1Of Biosystem,","
R4560 T5160 T5165 arg1Of Biosystem,","
R4561 T5160 T5166 arg1Of Biosystem,USA
R4562 T5162 T5161 arg2Of Sunnyvale,","
R4563 T5162 T5163 arg1Of Sunnyvale,","
R4564 T5164 T5163 arg2Of CA,","
R4565 T5167 T5158 arg3Of ),(
R4566 T5169 T5168 arg1Of ratio,The
R4567 T5169 T5170 arg1Of ratio,of
R4568 T5169 T5174 arg1Of ratio,to
R4569 T5169 T5177 arg1Of ratio,was
R4570 T5169 T5178 arg2Of ratio,expressed
R4571 T5173 T5170 arg2Of activity,of
R4572 T5173 T5171 arg1Of activity,firefly
R4573 T5173 T5172 arg1Of activity,luciferase
R4574 T5176 T5174 arg2Of activity,to
R4575 T5176 T5175 arg1Of activity,β-galactosidase
R4576 T5178 T5177 arg2Of expressed,was
R4577 T5178 T5179 arg1Of expressed,as
R4578 T5182 T5179 arg2Of units,as
R4579 T5182 T5180 arg1Of units,relative
R4580 T5182 T5181 arg1Of units,light

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T5199 0-5 NNS denotes Cells
T5200 5-6 , denotes ,
T5201 7-19 NN denotes transfection
T5202 19-20 , denotes ,
T5203 21-31 NNS denotes treatments
T5204 32-35 CC denotes and
T5205 36-46 NN denotes luciferase
T5206 47-52 NN denotes assay
T5207 53-57 NNP denotes HeLa
T5208 57-58 , denotes ,
T5209 59-62 CD denotes p50
T5210 62-63 FW denotes −
T5211 63-64 FW denotes /
T5212 64-65 FW denotes −
T5213 65-68 FW denotes p65
T5214 68-69 FW denotes −
T5215 69-70 FW denotes /
T5216 70-71 FW denotes −
T5217 72-77 NN denotes mouse
T5218 78-87 JJ denotes embryonic
T5219 88-99 NNS denotes fibroblasts
T5220 100-101 -LRB- denotes (
T5221 101-105 NNS denotes MEFs
T5222 105-106 -RRB- denotes )
T5223 107-108 -LRB- denotes (
T5224 108-110 CD denotes 52
T5225 110-111 -RRB- denotes )
T5226 112-115 CC denotes and
T5227 116-120 JJ denotes 293T
T5228 121-126 NNS denotes cells
T5229 127-131 VBD denotes were
T5230 132-140 VBN denotes cultured
T5231 141-143 IN denotes in
T5232 144-152 NNP denotes Dulbecco
T5233 152-154 POS denotes 's
T5234 155-163 VBN denotes modified
T5235 164-169 NNP denotes Eagle
T5236 169-171 POS denotes 's
T5237 172-178 NN denotes medium
T5238 178-179 : denotes ;
T5239 180-186 NNP denotes Jurkat
T5240 186-187 , denotes ,
T5241 188-192 NNP denotes U937
T5242 193-198 NNS denotes cells
T5243 199-202 CC denotes and
T5244 203-208 JJ denotes human
T5245 209-219 JJ denotes peripheral
T5246 220-225 NN denotes blood
T5247 226-237 NN denotes mononuclear
T5248 238-243 NNS denotes cells
T5249 244-245 -LRB- denotes (
T5250 245-250 NNS denotes PBMCs
T5251 250-251 -RRB- denotes )
T5252 252-256 VBD denotes were
T5253 257-265 VBN denotes cultured
T5254 266-268 IN denotes in
T5255 269-273 NNP denotes RPMI
T5256 274-278 CD denotes 1640
T5257 278-279 . denotes .
T5258 280-285 NNS denotes PBMCs
T5259 286-290 VBD denotes were
T5260 291-299 VBN denotes isolated
T5261 300-302 IN denotes as
T5262 303-313 RB denotes previously
T5263 314-323 VBN denotes described
T5264 324-325 -LRB- denotes (
T5265 325-327 CD denotes 53
T5266 327-328 -RRB- denotes )
T5267 328-329 . denotes .
T5268 330-335 NNP denotes Media
T5269 336-340 VBD denotes were
T5270 341-353 VBN denotes supplemented
T5271 354-358 IN denotes with
T5272 359-361 CD denotes 10
T5273 361-362 NN denotes %
T5274 363-379 JJ denotes heat-inactivated
T5275 380-385 JJ denotes fetal
T5276 386-390 NN denotes calf
T5277 391-396 NN denotes serum
T5278 397-400 CC denotes and
T5279 401-402 CD denotes 2
T5280 403-405 NNP denotes mM
T5281 406-417 NN denotes l-glutamine
T5282 418-419 -LRB- denotes (
T5283 419-424 NNP denotes Lonza
T5284 425-432 NNP denotes Cologne
T5285 433-435 NNP denotes AG
T5286 435-436 , denotes ,
T5287 437-444 NNP denotes Germany
T5288 444-445 -RRB- denotes )
T5289 445-446 . denotes .
T5290 447-451 NNP denotes HeLa
T5291 451-452 , denotes ,
T5292 453-456 CD denotes p50
T5293 456-457 FW denotes −
T5294 457-458 FW denotes /
T5295 458-459 FW denotes −
T5296 459-462 FW denotes p65
T5297 462-463 FW denotes −
T5298 463-464 FW denotes /
T5299 464-465 FW denotes −
T5300 466-470 NNPS denotes MEFs
T5301 471-474 CC denotes and
T5302 475-479 NNP denotes 293T
T5303 480-484 VBD denotes were
T5304 485-496 VBN denotes transfected
T5305 497-501 IN denotes with
T5306 502-505 NN denotes DNA
T5307 506-508 IN denotes by
T5308 509-514 VBG denotes using
T5309 515-521 NNP denotes FuGENE
T5310 522-524 NNP denotes HD
T5311 525-526 -LRB- denotes (
T5312 526-531 NNP denotes Roche
T5313 532-542 NNP denotes Diagnostic
T5314 543-547 NNP denotes GmbH
T5315 547-548 , denotes ,
T5316 549-557 NNP denotes Mannheim
T5317 557-558 , denotes ,
T5318 559-566 NNP denotes Germany
T5319 566-567 -RRB- denotes )
T5320 567-568 , denotes ,
T5321 569-578 VBG denotes according
T5322 579-581 TO denotes to
T5323 582-585 DT denotes the
T5324 586-598 NN denotes manufacturer
T5325 598-600 POS denotes 's
T5326 601-609 NN denotes protocol
T5327 609-610 : denotes ;
T5328 611-616 JJ denotes total
T5329 617-620 NNP denotes DNA
T5330 621-628 NNS denotes amounts
T5331 629-633 VBD denotes were
T5332 634-643 VBN denotes equalized
T5333 644-646 IN denotes by
T5334 647-659 NN denotes transfection
T5335 660-662 IN denotes of
T5336 663-670 NNP denotes pRc/CMV
T5337 671-676 JJ denotes empty
T5338 677-683 NN denotes vector
T5339 684-685 -LRB- denotes (
T5340 685-695 NNP denotes Invitrogen
T5341 695-696 , denotes ,
T5342 697-705 NNP denotes Carlsbad
T5343 705-706 , denotes ,
T5344 707-709 NNP denotes CA
T5345 709-710 , denotes ,
T5346 711-714 NNP denotes USA
T5347 714-715 -RRB- denotes )
T5348 715-716 . denotes .
T5349 717-720 IN denotes For
T5350 721-738 NN denotes pulse-stimulation
T5351 738-739 , denotes ,
T5352 740-744 NNP denotes HeLa
T5353 745-750 NNS denotes cells
T5354 751-755 VBD denotes were
T5355 756-763 VBN denotes treated
T5356 764-768 IN denotes with
T5357 769-776 NN denotes phorbol
T5358 777-789 JJ denotes 12-myristate
T5359 790-800 JJ denotes 13-acetate
T5360 801-802 -LRB- denotes (
T5361 802-805 NNP denotes PMA
T5362 805-806 : denotes ;
T5363 807-820 NNP denotes Sigma-Aldrich
T5364 820-821 , denotes ,
T5365 822-824 NNP denotes St
T5366 825-830 NNP denotes Louis
T5367 830-831 , denotes ,
T5368 832-834 NNP denotes MO
T5369 834-835 , denotes ,
T5370 836-839 NNP denotes USA
T5371 839-840 -RRB- denotes )
T5372 841-842 -LRB- denotes (
T5373 842-844 CD denotes 20
T5374 845-850 NN denotes ng/ml
T5375 850-851 -RRB- denotes )
T5376 852-855 IN denotes for
T5377 856-857 CD denotes 5
T5378 858-861 NN denotes min
T5379 861-862 , denotes ,
T5380 863-865 CC denotes or
T5381 866-871 NN denotes tumor
T5382 872-880 NN denotes necrosis
T5383 881-889 NN denotes factor-α
T5384 890-891 -LRB- denotes (
T5385 891-896 JJ denotes TNF-α
T5386 896-897 : denotes ;
T5387 898-911 NNP denotes Sigma-Aldrich
T5388 911-912 -RRB- denotes )
T5389 913-914 -LRB- denotes (
T5390 914-916 CD denotes 20
T5391 917-922 NN denotes ng/ml
T5392 922-923 -RRB- denotes )
T5393 924-927 IN denotes for
T5394 928-930 CD denotes 30
T5395 931-934 NN denotes min
T5396 934-935 , denotes ,
T5397 936-942 VBD denotes washed
T5398 943-948 RB denotes twice
T5399 949-951 IN denotes in
T5400 952-960 JJ denotes complete
T5401 961-968 NN denotes culture
T5402 969-975 NN denotes medium
T5403 976-979 CC denotes and
T5404 980-984 RB denotes then
T5405 985-993 VBD denotes returned
T5406 994-996 TO denotes to
T5407 997-1004 NN denotes culture
T5408 1004-1005 . denotes .
T5409 1006-1009 IN denotes For
T5410 1010-1020 NN denotes luciferase
T5411 1021-1027 NNS denotes assays
T5412 1027-1028 , denotes ,
T5413 1029-1038 JJ denotes pSV-β-Gal
T5414 1039-1042 VBD denotes was
T5415 1043-1057 VBN denotes co-transfected
T5416 1058-1062 IN denotes with
T5417 1063-1069 NNP denotes pκBluc
T5418 1070-1072 TO denotes to
T5419 1073-1080 VB denotes monitor
T5420 1081-1084 DT denotes the
T5421 1085-1097 NN denotes transfection
T5422 1098-1108 NN denotes efficiency
T5423 1108-1109 . denotes .
T5424 1110-1126 JJ denotes Forty-eight-hour
T5425 1127-1144 NN denotes post-transfection
T5426 1144-1145 , denotes ,
T5427 1146-1151 NNS denotes cells
T5428 1152-1156 VBD denotes were
T5429 1157-1162 VBN denotes lysed
T5430 1163-1165 IN denotes in
T5431 1166-1171 NN denotes lysis
T5432 1172-1178 NN denotes buffer
T5433 1179-1181 IN denotes of
T5434 1182-1186 NNP denotes Dual
T5435 1187-1192 NNP denotes Light
T5436 1193-1203 NNP denotes Luciferase
T5437 1204-1210 NNP denotes System
T5438 1211-1212 -LRB- denotes (
T5439 1212-1218 NNP denotes Tropix
T5440 1218-1219 , denotes ,
T5441 1220-1227 NNP denotes Bedford
T5442 1227-1228 , denotes ,
T5443 1229-1231 NNP denotes MA
T5444 1231-1232 , denotes ,
T5445 1233-1236 NNP denotes USA
T5446 1236-1237 -RRB- denotes )
T5447 1238-1241 CC denotes and
T5448 1242-1245 DT denotes the
T5449 1246-1256 NN denotes luciferase
T5450 1257-1260 CC denotes and
T5451 1261-1276 JJ denotes β-galactosidase
T5452 1277-1287 NNS denotes activities
T5453 1288-1292 VBD denotes were
T5454 1293-1302 VBN denotes evaluated
T5455 1303-1305 IN denotes by
T5456 1306-1311 VBG denotes using
T5457 1312-1316 NNP denotes Dual
T5458 1317-1322 NNP denotes Light
T5459 1323-1333 NNP denotes Luciferase
T5460 1334-1340 NNP denotes System
T5461 1341-1342 -LRB- denotes (
T5462 1342-1348 NNP denotes Tropix
T5463 1348-1349 -RRB- denotes )
T5464 1350-1352 IN denotes in
T5465 1353-1354 DT denotes a
T5466 1355-1369 NN denotes bioluminometer
T5467 1370-1371 -LRB- denotes (
T5468 1371-1377 NNP denotes Turner
T5469 1378-1387 NNP denotes Biosystem
T5470 1387-1388 , denotes ,
T5471 1389-1398 NNP denotes Sunnyvale
T5472 1398-1399 , denotes ,
T5473 1400-1402 NNP denotes CA
T5474 1402-1403 , denotes ,
T5475 1404-1407 NNP denotes USA
T5476 1407-1408 -RRB- denotes )
T5477 1408-1409 . denotes .
T5478 1410-1413 DT denotes The
T5479 1414-1419 NN denotes ratio
T5480 1420-1422 IN denotes of
T5481 1423-1430 RB denotes firefly
T5482 1431-1441 JJ denotes luciferase
T5483 1442-1450 NN denotes activity
T5484 1451-1453 TO denotes to
T5485 1454-1469 JJ denotes β-galactosidase
T5486 1470-1478 NN denotes activity
T5487 1479-1482 VBD denotes was
T5488 1483-1492 VBN denotes expressed
T5489 1493-1495 IN denotes as
T5490 1496-1504 JJ denotes relative
T5491 1505-1510 JJ denotes light
T5492 1511-1516 NNS denotes units
T5493 1516-1517 . denotes .
R4581 T5199 T5207 nmod Cells,HeLa
R4582 T5200 T5199 punct ",",Cells
R4583 T5201 T5199 conj transfection,Cells
R4584 T5202 T5201 punct ",",transfection
R4585 T5203 T5201 conj treatments,transfection
R4586 T5204 T5203 cc and,treatments
R4587 T5205 T5206 compound luciferase,assay
R4588 T5206 T5203 conj assay,treatments
R4589 T5207 T5230 nsubjpass HeLa,cultured
R4590 T5208 T5207 punct ",",HeLa
R4591 T5209 T5219 nummod p50,fibroblasts
R4592 T5210 T5219 nmod −,fibroblasts
R4593 T5211 T5219 nmod /,fibroblasts
R4594 T5212 T5219 nmod −,fibroblasts
R4595 T5213 T5219 nmod p65,fibroblasts
R4596 T5214 T5219 nmod −,fibroblasts
R4597 T5215 T5219 nmod /,fibroblasts
R4598 T5216 T5219 nmod −,fibroblasts
R4599 T5217 T5219 nmod mouse,fibroblasts
R4600 T5218 T5219 amod embryonic,fibroblasts
R4601 T5219 T5207 appos fibroblasts,HeLa
R4602 T5220 T5219 punct (,fibroblasts
R4603 T5221 T5219 appos MEFs,fibroblasts
R4604 T5222 T5219 punct ),fibroblasts
R4605 T5223 T5219 punct (,fibroblasts
R4606 T5224 T5219 appos 52,fibroblasts
R4607 T5225 T5219 punct ),fibroblasts
R4608 T5226 T5219 cc and,fibroblasts
R4609 T5227 T5228 amod 293T,cells
R4610 T5228 T5219 conj cells,fibroblasts
R4611 T5229 T5230 auxpass were,cultured
R4612 T5230 T5253 ccomp cultured,cultured
R4613 T5231 T5230 prep in,cultured
R4614 T5232 T5237 poss Dulbecco,medium
R4615 T5233 T5232 case 's,Dulbecco
R4616 T5234 T5237 amod modified,medium
R4617 T5235 T5237 poss Eagle,medium
R4618 T5236 T5235 case 's,Eagle
R4619 T5237 T5231 pobj medium,in
R4620 T5238 T5253 punct ;,cultured
R4621 T5239 T5253 nsubjpass Jurkat,cultured
R4622 T5240 T5239 punct ",",Jurkat
R4623 T5241 T5242 compound U937,cells
R4624 T5242 T5239 appos cells,Jurkat
R4625 T5243 T5242 cc and,cells
R4626 T5244 T5246 amod human,blood
R4627 T5245 T5246 compound peripheral,blood
R4628 T5246 T5248 compound blood,cells
R4629 T5247 T5248 compound mononuclear,cells
R4630 T5248 T5242 conj cells,cells
R4631 T5249 T5250 punct (,PBMCs
R4632 T5250 T5248 appos PBMCs,cells
R4633 T5251 T5248 punct ),cells
R4634 T5252 T5253 auxpass were,cultured
R4635 T5253 T5253 ROOT cultured,cultured
R4636 T5254 T5253 prep in,cultured
R4637 T5255 T5254 pobj RPMI,in
R4638 T5256 T5255 nummod 1640,RPMI
R4639 T5257 T5253 punct .,cultured
R4640 T5258 T5260 nsubjpass PBMCs,isolated
R4641 T5259 T5260 auxpass were,isolated
R4642 T5260 T5260 ROOT isolated,isolated
R4643 T5261 T5263 mark as,described
R4644 T5262 T5263 advmod previously,described
R4645 T5263 T5260 advcl described,isolated
R4646 T5264 T5263 punct (,described
R4647 T5265 T5263 npadvmod 53,described
R4648 T5266 T5263 punct ),described
R4649 T5267 T5260 punct .,isolated
R4650 T5268 T5270 nsubjpass Media,supplemented
R4651 T5269 T5270 auxpass were,supplemented
R4652 T5270 T5270 ROOT supplemented,supplemented
R4653 T5271 T5270 prep with,supplemented
R4654 T5272 T5273 nummod 10,%
R4655 T5273 T5277 nmod %,serum
R4656 T5274 T5277 amod heat-inactivated,serum
R4657 T5275 T5276 amod fetal,calf
R4658 T5276 T5277 compound calf,serum
R4659 T5277 T5271 pobj serum,with
R4660 T5278 T5277 cc and,serum
R4661 T5279 T5280 nummod 2,mM
R4662 T5280 T5281 compound mM,l-glutamine
R4663 T5281 T5277 conj l-glutamine,serum
R4664 T5282 T5285 punct (,AG
R4665 T5283 T5285 compound Lonza,AG
R4666 T5284 T5285 compound Cologne,AG
R4667 T5285 T5277 appos AG,serum
R4668 T5286 T5285 punct ",",AG
R4669 T5287 T5285 npadvmod Germany,AG
R4670 T5288 T5285 punct ),AG
R4671 T5289 T5270 punct .,supplemented
R4672 T5290 T5304 nsubjpass HeLa,transfected
R4673 T5291 T5290 punct ",",HeLa
R4674 T5292 T5300 nummod p50,MEFs
R4675 T5293 T5299 nmod −,−
R4676 T5294 T5299 nmod /,−
R4677 T5295 T5299 nmod −,−
R4678 T5296 T5299 nmod p65,−
R4679 T5297 T5299 nmod −,−
R4680 T5298 T5299 nmod /,−
R4681 T5299 T5300 nmod −,MEFs
R4682 T5300 T5290 conj MEFs,HeLa
R4683 T5301 T5300 cc and,MEFs
R4684 T5302 T5300 conj 293T,MEFs
R4685 T5303 T5304 auxpass were,transfected
R4686 T5304 T5332 ccomp transfected,equalized
R4687 T5305 T5304 prep with,transfected
R4688 T5306 T5305 pobj DNA,with
R4689 T5307 T5304 prep by,transfected
R4690 T5308 T5307 pcomp using,by
R4691 T5309 T5310 compound FuGENE,HD
R4692 T5310 T5308 dobj HD,using
R4693 T5311 T5314 punct (,GmbH
R4694 T5312 T5314 compound Roche,GmbH
R4695 T5313 T5314 compound Diagnostic,GmbH
R4696 T5314 T5308 dobj GmbH,using
R4697 T5315 T5314 punct ",",GmbH
R4698 T5316 T5314 conj Mannheim,GmbH
R4699 T5317 T5316 punct ",",Mannheim
R4700 T5318 T5314 npadvmod Germany,GmbH
R4701 T5319 T5314 punct ),GmbH
R4702 T5320 T5304 punct ",",transfected
R4703 T5321 T5304 prep according,transfected
R4704 T5322 T5321 prep to,according
R4705 T5323 T5324 det the,manufacturer
R4706 T5324 T5326 poss manufacturer,protocol
R4707 T5325 T5324 case 's,manufacturer
R4708 T5326 T5322 pobj protocol,to
R4709 T5327 T5332 punct ;,equalized
R4710 T5328 T5330 amod total,amounts
R4711 T5329 T5330 compound DNA,amounts
R4712 T5330 T5332 nsubjpass amounts,equalized
R4713 T5331 T5332 auxpass were,equalized
R4714 T5332 T5332 ROOT equalized,equalized
R4715 T5333 T5332 agent by,equalized
R4716 T5334 T5333 pobj transfection,by
R4717 T5335 T5334 prep of,transfection
R4718 T5336 T5338 nmod pRc/CMV,vector
R4719 T5337 T5338 amod empty,vector
R4720 T5338 T5335 pobj vector,of
R4721 T5339 T5340 punct (,Invitrogen
R4722 T5340 T5338 appos Invitrogen,vector
R4723 T5341 T5340 punct ",",Invitrogen
R4724 T5342 T5340 npadvmod Carlsbad,Invitrogen
R4725 T5343 T5342 punct ",",Carlsbad
R4726 T5344 T5342 conj CA,Carlsbad
R4727 T5345 T5344 punct ",",CA
R4728 T5346 T5344 conj USA,CA
R4729 T5347 T5340 punct ),Invitrogen
R4730 T5348 T5332 punct .,equalized
R4731 T5349 T5355 prep For,treated
R4732 T5350 T5349 pobj pulse-stimulation,For
R4733 T5351 T5355 punct ",",treated
R4734 T5352 T5353 compound HeLa,cells
R4735 T5353 T5355 nsubjpass cells,treated
R4736 T5354 T5355 auxpass were,treated
R4737 T5355 T5397 ccomp treated,washed
R4738 T5356 T5355 prep with,treated
R4739 T5357 T5359 nmod phorbol,13-acetate
R4740 T5358 T5359 amod 12-myristate,13-acetate
R4741 T5359 T5356 pobj 13-acetate,with
R4742 T5360 T5359 punct (,13-acetate
R4743 T5361 T5359 appos PMA,13-acetate
R4744 T5362 T5397 punct ;,washed
R4745 T5363 T5397 nsubj Sigma-Aldrich,washed
R4746 T5364 T5363 punct ",",Sigma-Aldrich
R4747 T5365 T5366 compound St,Louis
R4748 T5366 T5363 conj Louis,Sigma-Aldrich
R4749 T5367 T5366 punct ",",Louis
R4750 T5368 T5366 conj MO,Louis
R4751 T5369 T5368 punct ",",MO
R4752 T5370 T5368 conj USA,MO
R4753 T5371 T5363 punct ),Sigma-Aldrich
R4754 T5372 T5374 punct (,ng/ml
R4755 T5373 T5374 nummod 20,ng/ml
R4756 T5374 T5363 appos ng/ml,Sigma-Aldrich
R4757 T5375 T5363 punct ),Sigma-Aldrich
R4758 T5376 T5363 prep for,Sigma-Aldrich
R4759 T5377 T5378 nummod 5,min
R4760 T5378 T5376 pobj min,for
R4761 T5379 T5378 punct ",",min
R4762 T5380 T5378 cc or,min
R4763 T5381 T5382 compound tumor,necrosis
R4764 T5382 T5383 compound necrosis,factor-α
R4765 T5383 T5378 conj factor-α,min
R4766 T5384 T5385 punct (,TNF-α
R4767 T5385 T5383 appos TNF-α,factor-α
R4768 T5386 T5363 punct ;,Sigma-Aldrich
R4769 T5387 T5363 conj Sigma-Aldrich,Sigma-Aldrich
R4770 T5388 T5363 punct ),Sigma-Aldrich
R4771 T5389 T5391 punct (,ng/ml
R4772 T5390 T5391 nummod 20,ng/ml
R4773 T5391 T5363 appos ng/ml,Sigma-Aldrich
R4774 T5392 T5363 punct ),Sigma-Aldrich
R4775 T5393 T5363 prep for,Sigma-Aldrich
R4776 T5394 T5395 nummod 30,min
R4777 T5395 T5393 pobj min,for
R4778 T5396 T5397 punct ",",washed
R4779 T5397 T5397 ROOT washed,washed
R4780 T5398 T5397 advmod twice,washed
R4781 T5399 T5397 prep in,washed
R4782 T5400 T5402 amod complete,medium
R4783 T5401 T5402 compound culture,medium
R4784 T5402 T5399 pobj medium,in
R4785 T5403 T5397 cc and,washed
R4786 T5404 T5405 advmod then,returned
R4787 T5405 T5397 conj returned,washed
R4788 T5406 T5405 prep to,returned
R4789 T5407 T5406 pobj culture,to
R4790 T5408 T5397 punct .,washed
R4791 T5409 T5415 prep For,co-transfected
R4792 T5410 T5411 compound luciferase,assays
R4793 T5411 T5409 pobj assays,For
R4794 T5412 T5415 punct ",",co-transfected
R4795 T5413 T5414 nsubj pSV-β-Gal,was
R4796 T5414 T5415 auxpass was,co-transfected
R4797 T5415 T5415 ROOT co-transfected,co-transfected
R4798 T5416 T5415 prep with,co-transfected
R4799 T5417 T5416 pobj pκBluc,with
R4800 T5418 T5419 aux to,monitor
R4801 T5419 T5415 advcl monitor,co-transfected
R4802 T5420 T5422 det the,efficiency
R4803 T5421 T5422 compound transfection,efficiency
R4804 T5422 T5419 dobj efficiency,monitor
R4805 T5423 T5415 punct .,co-transfected
R4806 T5424 T5425 amod Forty-eight-hour,post-transfection
R4807 T5425 T5429 intj post-transfection,lysed
R4808 T5426 T5429 punct ",",lysed
R4809 T5427 T5429 nsubjpass cells,lysed
R4810 T5428 T5429 auxpass were,lysed
R4811 T5429 T5429 ROOT lysed,lysed
R4812 T5430 T5429 prep in,lysed
R4813 T5431 T5432 compound lysis,buffer
R4814 T5432 T5430 pobj buffer,in
R4815 T5433 T5432 prep of,buffer
R4816 T5434 T5435 compound Dual,Light
R4817 T5435 T5437 compound Light,System
R4818 T5436 T5437 compound Luciferase,System
R4819 T5437 T5433 pobj System,of
R4820 T5438 T5437 punct (,System
R4821 T5439 T5437 appos Tropix,System
R4822 T5440 T5439 punct ",",Tropix
R4823 T5441 T5439 conj Bedford,Tropix
R4824 T5442 T5441 punct ",",Bedford
R4825 T5443 T5441 conj MA,Bedford
R4826 T5444 T5443 punct ",",MA
R4827 T5445 T5443 conj USA,MA
R4828 T5446 T5443 punct ),MA
R4829 T5447 T5437 cc and,System
R4830 T5448 T5449 det the,luciferase
R4831 T5449 T5452 nmod luciferase,activities
R4832 T5450 T5449 cc and,luciferase
R4833 T5451 T5449 conj β-galactosidase,luciferase
R4834 T5452 T5454 nsubjpass activities,evaluated
R4835 T5453 T5454 auxpass were,evaluated
R4836 T5454 T5429 conj evaluated,lysed
R4837 T5455 T5454 prep by,evaluated
R4838 T5456 T5455 pcomp using,by
R4839 T5457 T5458 compound Dual,Light
R4840 T5458 T5460 compound Light,System
R4841 T5459 T5460 compound Luciferase,System
R4842 T5460 T5456 dobj System,using
R4843 T5461 T5460 punct (,System
R4844 T5462 T5460 appos Tropix,System
R4845 T5463 T5460 punct ),System
R4846 T5464 T5456 prep in,using
R4847 T5465 T5466 det a,bioluminometer
R4848 T5466 T5464 pobj bioluminometer,in
R4849 T5467 T5469 punct (,Biosystem
R4850 T5468 T5469 compound Turner,Biosystem
R4851 T5469 T5454 npadvmod Biosystem,evaluated
R4852 T5470 T5469 punct ",",Biosystem
R4853 T5471 T5469 npadvmod Sunnyvale,Biosystem
R4854 T5472 T5471 punct ",",Sunnyvale
R4855 T5473 T5471 conj CA,Sunnyvale
R4856 T5474 T5473 punct ",",CA
R4857 T5475 T5473 conj USA,CA
R4858 T5476 T5473 punct ),CA
R4859 T5477 T5454 punct .,evaluated
R4860 T5478 T5479 det The,ratio
R4861 T5479 T5488 nsubjpass ratio,expressed
R4862 T5480 T5479 prep of,ratio
R4863 T5481 T5483 amod firefly,activity
R4864 T5482 T5483 amod luciferase,activity
R4865 T5483 T5480 pobj activity,of
R4866 T5484 T5483 prep to,activity
R4867 T5485 T5486 amod β-galactosidase,activity
R4868 T5486 T5484 pobj activity,to
R4869 T5487 T5488 auxpass was,expressed
R4870 T5488 T5488 ROOT expressed,expressed
R4871 T5489 T5488 prep as,expressed
R4872 T5490 T5492 amod relative,units
R4873 T5491 T5492 amod light,units
R4874 T5492 T5489 pobj units,as
R4875 T5493 T5488 punct .,expressed

UBERON-AE

Id Subject Object Predicate Lexical cue
T4890 220-225 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T4891 391-396 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T5503 707-709 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T5504 1400-1402 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T5505 872-880 http://purl.obolibrary.org/obo/GO_0001906 denotes necrosis
T5506 872-880 http://purl.obolibrary.org/obo/GO_0008219 denotes necrosis
T5507 872-880 http://purl.obolibrary.org/obo/GO_0019835 denotes necrosis
T5508 1166-1171 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T5509 872-880 http://purl.obolibrary.org/obo/GO_0070265 denotes necrosis
T5510 1261-1287 http://purl.obolibrary.org/obo/GO_0004565 denotes β-galactosidase activities
T5511 1442-1469 http://purl.obolibrary.org/obo/GO_0004565 denotes activity to β-galactosidase
T5512 1261-1287 http://purl.obolibrary.org/obo/GO_0004336 denotes β-galactosidase activities
T5513 1442-1469 http://purl.obolibrary.org/obo/GO_0004336 denotes activity to β-galactosidase
T5514 1261-1287 http://purl.obolibrary.org/obo/GO_1903771 denotes β-galactosidase activities
T5515 1442-1469 http://purl.obolibrary.org/obo/GO_1903771 denotes activity to β-galactosidase
T5516 1261-1287 http://purl.obolibrary.org/obo/GO_1903770 denotes β-galactosidase activities
T5517 1442-1469 http://purl.obolibrary.org/obo/GO_1903770 denotes activity to β-galactosidase
T5518 1261-1287 http://purl.obolibrary.org/obo/GO_0033920 denotes β-galactosidase activities
T5519 1442-1469 http://purl.obolibrary.org/obo/GO_0033920 denotes activity to β-galactosidase
T5520 1261-1287 http://purl.obolibrary.org/obo/GO_0033929 denotes β-galactosidase activities
T5521 1442-1469 http://purl.obolibrary.org/obo/GO_0033929 denotes activity to β-galactosidase
T5522 1261-1287 http://purl.obolibrary.org/obo/GO_0033930 denotes β-galactosidase activities
T5523 1442-1469 http://purl.obolibrary.org/obo/GO_0033930 denotes activity to β-galactosidase
T5524 1431-1450 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T5525 1431-1450 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T5526 1431-1450 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T5527 1431-1450 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T5528 1431-1450 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity

GO-MF

Id Subject Object Predicate Lexical cue
T5529 707-709 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T5530 1400-1402 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T5531 1261-1287 http://purl.obolibrary.org/obo/GO_0004565 denotes β-galactosidase activities
T5532 1442-1469 http://purl.obolibrary.org/obo/GO_0004565 denotes activity to β-galactosidase
T5533 1261-1287 http://purl.obolibrary.org/obo/GO_0004336 denotes β-galactosidase activities
T5534 1442-1469 http://purl.obolibrary.org/obo/GO_0004336 denotes activity to β-galactosidase
T5535 1261-1287 http://purl.obolibrary.org/obo/GO_0033920 denotes β-galactosidase activities
T5536 1442-1469 http://purl.obolibrary.org/obo/GO_0033920 denotes activity to β-galactosidase
T5537 1261-1287 http://purl.obolibrary.org/obo/GO_0033929 denotes β-galactosidase activities
T5538 1442-1469 http://purl.obolibrary.org/obo/GO_0033929 denotes activity to β-galactosidase
T5539 1261-1287 http://purl.obolibrary.org/obo/GO_0033930 denotes β-galactosidase activities
T5540 1442-1469 http://purl.obolibrary.org/obo/GO_0033930 denotes activity to β-galactosidase
T5541 1431-1450 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T5542 1431-1450 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T5543 1431-1450 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T5544 1431-1450 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T5545 1431-1450 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity

GO-CC

Id Subject Object Predicate Lexical cue
T5546 121-126 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5547 193-198 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5548 238-243 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5549 745-750 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5550 1146-1151 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T4906 0-52 Sentence denotes Cells, transfection, treatments and luciferase assay
T4907 53-279 Sentence denotes HeLa, p50−/−p65−/− mouse embryonic fibroblasts (MEFs) (52) and 293T cells were cultured in Dulbecco's modified Eagle's medium; Jurkat, U937 cells and human peripheral blood mononuclear cells (PBMCs) were cultured in RPMI 1640.
T4908 280-329 Sentence denotes PBMCs were isolated as previously described (53).
T4909 330-446 Sentence denotes Media were supplemented with 10% heat-inactivated fetal calf serum and 2 mM l-glutamine (Lonza Cologne AG, Germany).
T4910 447-716 Sentence denotes HeLa, p50−/−p65−/− MEFs and 293T were transfected with DNA by using FuGENE HD (Roche Diagnostic GmbH, Mannheim, Germany), according to the manufacturer's protocol; total DNA amounts were equalized by transfection of pRc/CMV empty vector (Invitrogen, Carlsbad, CA, USA).
T4911 717-1005 Sentence denotes For pulse-stimulation, HeLa cells were treated with phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, St Louis, MO, USA) (20 ng/ml) for 5 min, or tumor necrosis factor-α (TNF-α; Sigma-Aldrich) (20 ng/ml) for 30 min, washed twice in complete culture medium and then returned to culture.
T4912 1006-1109 Sentence denotes For luciferase assays, pSV-β-Gal was co-transfected with pκBluc to monitor the transfection efficiency.
T4913 1110-1409 Sentence denotes Forty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA).
T4914 1410-1517 Sentence denotes The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.
T41 0-52 Sentence denotes Cells, transfection, treatments and luciferase assay
T42 53-279 Sentence denotes HeLa, p50−/−p65−/− mouse embryonic fibroblasts (MEFs) (52) and 293T cells were cultured in Dulbecco's modified Eagle's medium; Jurkat, U937 cells and human peripheral blood mononuclear cells (PBMCs) were cultured in RPMI 1640.
T43 280-329 Sentence denotes PBMCs were isolated as previously described (53).
T44 330-446 Sentence denotes Media were supplemented with 10% heat-inactivated fetal calf serum and 2 mM l-glutamine (Lonza Cologne AG, Germany).
T45 447-716 Sentence denotes HeLa, p50−/−p65−/− MEFs and 293T were transfected with DNA by using FuGENE HD (Roche Diagnostic GmbH, Mannheim, Germany), according to the manufacturer's protocol; total DNA amounts were equalized by transfection of pRc/CMV empty vector (Invitrogen, Carlsbad, CA, USA).
T46 717-1005 Sentence denotes For pulse-stimulation, HeLa cells were treated with phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, St Louis, MO, USA) (20 ng/ml) for 5 min, or tumor necrosis factor-α (TNF-α; Sigma-Aldrich) (20 ng/ml) for 30 min, washed twice in complete culture medium and then returned to culture.
T47 1006-1109 Sentence denotes For luciferase assays, pSV-β-Gal was co-transfected with pκBluc to monitor the transfection efficiency.
T48 1110-1409 Sentence denotes Forty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA).
T49 1410-1517 Sentence denotes The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.

events-check-again

Id Subject Object Predicate Lexical cue
T5622 36-46 Protein denotes luciferase
T5623 59-62 Protein denotes p50
T5624 65-68 Protein denotes p65
T5625 453-456 Protein denotes p50
T5626 459-462 Protein denotes p65
T5627 866-889 Protein denotes tumor necrosis factor-α
T5628 891-896 Protein denotes TNF-α
T5629 1010-1020 Protein denotes luciferase
T5630 1033-1038 Protein denotes β-Gal
T5631 1193-1203 Protein denotes Luciferase
T5632 1246-1256 Protein denotes luciferase
T5633 1261-1276 Protein denotes β-galactosidase
T5634 1431-1441 Protein denotes luciferase
T5635 1454-1469 Protein denotes β-galactosidase
R4882 T5628 T5627 equivalentTo TNF-α,tumor necrosis factor-α

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T5636 36-46 Protein denotes luciferase
T5637 515-524 Protein denotes FuGENE HD
T5638 866-889 Protein denotes tumor necrosis factor-α
T5639 891-896 Protein denotes TNF-α
T5640 898-911 Protein denotes Sigma-Aldrich
T5641 1010-1020 Protein denotes luciferase
T5642 1063-1069 Protein denotes pκBluc
T5643 1043-1057 Gene_expression denotes co-transfected
T5644 1043-1057 Gene_expression denotes co-transfected
T5645 1043-1057 Positive_regulation denotes co-transfected
T5646 1043-1057 Positive_regulation denotes co-transfected
T5647 1187-1203 Protein denotes Light Luciferase
T5648 1246-1256 Protein denotes luciferase
T5649 1261-1276 Protein denotes β-galactosidase
T5650 1317-1333 Protein denotes Light Luciferase
T5651 1431-1441 Protein denotes luciferase
T5652 1454-1469 Protein denotes β-galactosidase
R4883 T5641 T5643 themeOf luciferase,co-transfected
R4884 T5642 T5644 themeOf pκBluc,co-transfected
R4885 T5643 T5645 themeOf co-transfected,co-transfected
R4886 T5644 T5646 themeOf co-transfected,co-transfected

test2

Id Subject Object Predicate Lexical cue
T4875 36-46 Protein denotes luciferase
T4876 59-62 Protein denotes p50
T4877 65-68 Protein denotes p65
T4878 453-456 Protein denotes p50
T4879 459-462 Protein denotes p65
T4880 866-889 Protein denotes tumor necrosis factor-α
T4881 891-896 Protein denotes TNF-α
T4882 1010-1020 Protein denotes luciferase
T4883 1033-1038 Protein denotes β-Gal
T4884 1193-1203 Protein denotes Luciferase
T4885 1246-1256 Protein denotes luciferase
T4886 1261-1276 Protein denotes β-galactosidase
T4887 1431-1441 Protein denotes luciferase
T4888 1454-1469 Protein denotes β-galactosidase
T4889 1483-1492 Gene_expression denotes expressed
R4306 T4881 T4880 equivalentTo TNF-α,tumor necrosis factor-α

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T4892 36-46 Protein denotes luciferase
T4893 59-62 Protein denotes p50
T4894 65-68 Protein denotes p65
T4895 453-456 Protein denotes p50
T4896 459-462 Protein denotes p65
T4897 866-889 Protein denotes tumor necrosis factor-α
T4898 891-896 Protein denotes TNF-α
T4899 1010-1020 Protein denotes luciferase
T4900 1033-1038 Protein denotes β-Gal
T4901 1193-1203 Protein denotes Luciferase
T4902 1246-1256 Protein denotes luciferase
T4903 1261-1276 Protein denotes β-galactosidase
T4904 1431-1441 Protein denotes luciferase
T4905 1454-1469 Protein denotes β-galactosidase
R4307 T4898 T4897 equivalentTo TNF-α,tumor necrosis factor-α

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T5183 36-46 P08659 denotes luciferase
T5184 59-62 P19838 denotes p50
T5185 65-68 Q04206 denotes p65
T5186 65-68 P21579 denotes p65
T5187 453-456 P19838 denotes p50
T5188 459-462 P21579 denotes p65
T5189 459-462 Q04206 denotes p65
T5190 866-887 P01375 denotes tumor necrosis factor
T5191 891-894 P01375 denotes TNF
T5192 891-896 P01375 denotes TNF-α
T5193 1010-1020 P08659 denotes luciferase
T5194 1193-1203 P08659 denotes Luciferase
T5195 1246-1256 P08659 denotes luciferase
T5196 1323-1333 P08659 denotes Luciferase
T5197 1431-1441 P08659 denotes luciferase
T5198 1496-1504 Q04864 denotes relative