PMC:3333881 / 27369-29092
Annnotations
bionlp-st-ge-2016-coref
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26503 | 45-48 | Anaphor | denotes | its |
| T26504 | 30-33 | Antecedent | denotes | p65 |
| R20924 | T26503 | T26504 | boundBy | its,p65 |
bionlp-st-ge-2016-spacy-parsed
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26585 | 10-13 | NNP | denotes | Tat |
| T26586 | 14-24 | NNS | denotes | associates |
| T26587 | 25-29 | IN | denotes | with |
| T26588 | 30-33 | CD | denotes | p65 |
| T26589 | 34-44 | VBG | denotes | increasing |
| T26590 | 45-48 | PRP$ | denotes | its |
| T26591 | 49-61 | JJ | denotes | DNA-affinity |
| T26592 | 62-69 | JJ | denotes | binding |
| T26593 | 69-70 | . | denotes | . |
| T26594 | 71-72 | -LRB- | denotes | ( |
| T26595 | 72-73 | NNP | denotes | A |
| T26596 | 73-74 | -RRB- | denotes | ) |
| T26597 | 75-79 | NNP | denotes | HeLa |
| T26598 | 80-85 | NNS | denotes | cells |
| T26599 | 86-90 | VBD | denotes | were |
| T26600 | 91-102 | VBN | denotes | transfected |
| T26601 | 103-107 | IN | denotes | with |
| T26602 | 108-115 | NNP | denotes | pRc/CMV |
| T26603 | 115-117 | CD | denotes | -3 |
| T26604 | 117-124 | NNP | denotes | xHA-p65 |
| T26605 | 125-126 | -LRB- | denotes | ( |
| T26606 | 126-127 | CD | denotes | 5 |
| T26607 | 128-130 | NN | denotes | µg |
| T26608 | 130-131 | -RRB- | denotes | ) |
| T26609 | 132-134 | IN | denotes | in |
| T26610 | 135-143 | NN | denotes | presence |
| T26611 | 144-146 | CC | denotes | or |
| T26612 | 147-154 | NN | denotes | absence |
| T26613 | 155-157 | IN | denotes | of |
| T26614 | 158-169 | NN | denotes | p3xFLAG-Tat |
| T26615 | 169-170 | , | denotes | , |
| T26616 | 171-182 | NN | denotes | p3xFLAG-Tat |
| T26617 | 183-184 | NNP | denotes | T |
| T26618 | 184-185 | , | denotes | , |
| T26619 | 185-186 | NNP | denotes | N |
| T26620 | 186-187 | -LRB- | denotes | ( |
| T26621 | 187-192 | CD | denotes | 23,24 |
| T26622 | 192-193 | -RRB- | denotes | ) |
| T26623 | 193-194 | DT | denotes | A |
| T26624 | 194-195 | , | denotes | , |
| T26625 | 196-207 | JJ | denotes | p3xFLAG-Tat |
| T26626 | 208-209 | NNP | denotes | K |
| T26627 | 209-210 | -LRB- | denotes | ( |
| T26628 | 210-215 | CD | denotes | 50,51 |
| T26629 | 215-216 | -RRB- | denotes | ) |
| T26630 | 216-217 | DT | denotes | A |
| T26631 | 217-218 | , | denotes | , |
| T26632 | 219-230 | JJ | denotes | p3xFLAG-Tat |
| T26633 | 231-232 | NN | denotes | R |
| T26634 | 232-233 | -LRB- | denotes | ( |
| T26635 | 233-235 | CD | denotes | 49 |
| T26636 | 236-238 | CD | denotes | 57 |
| T26637 | 238-239 | -RRB- | denotes | ) |
| T26638 | 239-240 | DT | denotes | A |
| T26639 | 240-241 | , | denotes | , |
| T26640 | 242-244 | CC | denotes | or |
| T26641 | 245-256 | NN | denotes | p3xFLAG-Tat |
| T26642 | 257-258 | NNP | denotes | C |
| T26643 | 258-259 | -LRB- | denotes | ( |
| T26644 | 259-267 | CD | denotes | 22,25,27 |
| T26645 | 267-268 | -RRB- | denotes | ) |
| T26646 | 268-269 | DT | denotes | A |
| T26647 | 270-271 | -LRB- | denotes | ( |
| T26648 | 271-272 | CD | denotes | 5 |
| T26649 | 273-275 | NN | denotes | µg |
| T26650 | 275-276 | -RRB- | denotes | ) |
| T26651 | 276-277 | . | denotes | . |
| T26652 | 278-286 | JJ | denotes | FLAG-Tat |
| T26653 | 287-295 | NNS | denotes | proteins |
| T26654 | 296-300 | VBD | denotes | were |
| T26655 | 301-319 | VBN | denotes | immunoprecipitated |
| T26656 | 320-324 | IN | denotes | with |
| T26657 | 325-334 | JJ | denotes | anti-FLAG |
| T26658 | 335-343 | NN | denotes | antibody |
| T26659 | 343-344 | : | denotes | ; |
| T26660 | 345-360 | NNS | denotes | immunocomplexes |
| T26661 | 361-365 | VBD | denotes | were |
| T26662 | 366-375 | VBN | denotes | separated |
| T26663 | 376-378 | IN | denotes | by |
| T26664 | 379-381 | CD | denotes | 12 |
| T26665 | 381-382 | NN | denotes | % |
| T26666 | 383-386 | NNP | denotes | SDS |
| T26667 | 387-391 | NNP | denotes | PAGE |
| T26668 | 392-395 | CC | denotes | and |
| T26669 | 396-404 | VBN | denotes | analysed |
| T26670 | 405-407 | IN | denotes | by |
| T26671 | 408-415 | JJ | denotes | western |
| T26672 | 416-424 | VBG | denotes | blotting |
| T26673 | 425-429 | IN | denotes | with |
| T26674 | 430-439 | JJ | denotes | anti-FLAG |
| T26675 | 440-442 | CC | denotes | or |
| T26676 | 443-450 | JJ | denotes | anti-HA |
| T26677 | 451-461 | NNS | denotes | antibodies |
| T26678 | 461-462 | . | denotes | . |
| T26679 | 463-464 | -LRB- | denotes | ( |
| T26680 | 464-465 | NNP | denotes | B |
| T26681 | 465-466 | -RRB- | denotes | ) |
| T26682 | 467-469 | IN | denotes | In |
| T26683 | 470-475 | NN | denotes | vitro |
| T26684 | 476-486 | VBN | denotes | translated |
| T26685 | 487-490 | NNS | denotes | p65 |
| T26686 | 491-492 | -LRB- | denotes | ( |
| T26687 | 492-493 | CD | denotes | 5 |
| T26688 | 494-496 | NN | denotes | µl |
| T26689 | 496-497 | -RRB- | denotes | ) |
| T26690 | 498-501 | VBD | denotes | was |
| T26691 | 502-511 | VBN | denotes | incubated |
| T26692 | 512-516 | IN | denotes | with |
| T26693 | 517-524 | NNP | denotes | GST-Tat |
| T26694 | 524-525 | , | denotes | , |
| T26695 | 526-533 | NNP | denotes | GST-Tat |
| T26696 | 534-535 | NNP | denotes | T |
| T26697 | 535-536 | , | denotes | , |
| T26698 | 536-537 | NNP | denotes | N |
| T26699 | 537-538 | -LRB- | denotes | ( |
| T26700 | 538-543 | CD | denotes | 23,24 |
| T26701 | 543-544 | -RRB- | denotes | ) |
| T26702 | 544-545 | DT | denotes | A |
| T26703 | 545-546 | , | denotes | , |
| T26704 | 547-554 | NNP | denotes | GST-Tat |
| T26705 | 555-556 | NNP | denotes | K |
| T26706 | 556-557 | -LRB- | denotes | ( |
| T26707 | 557-562 | CD | denotes | 50,51 |
| T26708 | 562-563 | -RRB- | denotes | ) |
| T26709 | 563-564 | DT | denotes | A |
| T26710 | 564-565 | , | denotes | , |
| T26711 | 566-573 | NNP | denotes | GST-Tat |
| T26712 | 574-575 | NNP | denotes | R |
| T26713 | 575-576 | -LRB- | denotes | ( |
| T26714 | 576-578 | CD | denotes | 49 |
| T26715 | 579-581 | CD | denotes | 57 |
| T26716 | 581-582 | -RRB- | denotes | ) |
| T26717 | 582-583 | DT | denotes | A |
| T26718 | 583-584 | , | denotes | , |
| T26719 | 585-592 | NNP | denotes | GST-Tat |
| T26720 | 593-594 | NNP | denotes | C |
| T26721 | 594-595 | -LRB- | denotes | ( |
| T26722 | 595-603 | CD | denotes | 22,25,27 |
| T26723 | 603-604 | -RRB- | denotes | ) |
| T26724 | 604-605 | DT | denotes | A |
| T26725 | 605-606 | , | denotes | , |
| T26726 | 607-609 | CC | denotes | or |
| T26727 | 610-613 | NNP | denotes | GST |
| T26728 | 614-615 | -LRB- | denotes | ( |
| T26729 | 615-616 | CD | denotes | 5 |
| T26730 | 617-619 | NN | denotes | µg |
| T26731 | 619-620 | -RRB- | denotes | ) |
| T26732 | 621-631 | VBN | denotes | conjugated |
| T26733 | 632-636 | IN | denotes | with |
| T26734 | 637-658 | NNP | denotes | Glutathione-Sepharose |
| T26735 | 658-659 | . | denotes | . |
| T26736 | 660-667 | NN | denotes | Protein |
| T26737 | 668-677 | NNS | denotes | complexes |
| T26738 | 678-682 | VBD | denotes | were |
| T26739 | 683-692 | VBN | denotes | recovered |
| T26740 | 693-695 | IN | denotes | by |
| T26741 | 696-704 | NNP | denotes | GST-pull |
| T26742 | 705-709 | RB | denotes | down |
| T26743 | 709-710 | , | denotes | , |
| T26744 | 711-720 | VBN | denotes | separated |
| T26745 | 721-723 | IN | denotes | by |
| T26746 | 724-726 | CD | denotes | 12 |
| T26747 | 726-727 | NN | denotes | % |
| T26748 | 728-731 | NNP | denotes | SDS |
| T26749 | 732-736 | NNP | denotes | PAGE |
| T26750 | 736-737 | , | denotes | , |
| T26751 | 738-741 | CC | denotes | and |
| T26752 | 742-750 | VBN | denotes | analysed |
| T26753 | 751-753 | IN | denotes | by |
| T26754 | 754-761 | JJ | denotes | western |
| T26755 | 762-770 | VBG | denotes | blotting |
| T26756 | 771-775 | IN | denotes | with |
| T26757 | 776-784 | JJ | denotes | anti-p65 |
| T26758 | 785-787 | CC | denotes | or |
| T26759 | 788-796 | JJ | denotes | anti-GST |
| T26760 | 797-807 | NNS | denotes | antibodies |
| T26761 | 807-808 | . | denotes | . |
| T26762 | 809-810 | -LRB- | denotes | ( |
| T26763 | 810-811 | $ | denotes | C |
| T26764 | 811-812 | -RRB- | denotes | ) |
| T26765 | 813-822 | JJ | denotes | Schematic |
| T26766 | 823-837 | NN | denotes | representation |
| T26767 | 838-840 | IN | denotes | of |
| T26768 | 841-850 | JJ | denotes | wild-type |
| T26769 | 851-854 | CC | denotes | and |
| T26770 | 855-861 | JJ | denotes | mutant |
| T26771 | 862-865 | NNS | denotes | p65 |
| T26772 | 866-874 | NNS | denotes | proteins |
| T26773 | 874-875 | . | denotes | . |
| T26774 | 876-877 | -LRB- | denotes | ( |
| T26775 | 877-878 | NNP | denotes | D |
| T26776 | 878-879 | -RRB- | denotes | ) |
| T26777 | 880-884 | NNP | denotes | HeLa |
| T26778 | 885-890 | NNS | denotes | cells |
| T26779 | 891-895 | VBD | denotes | were |
| T26780 | 896-907 | VBN | denotes | transfected |
| T26781 | 908-912 | IN | denotes | with |
| T26782 | 913-924 | NN | denotes | p3xFLAG-Tat |
| T26783 | 925-926 | -LRB- | denotes | ( |
| T26784 | 926-927 | CD | denotes | 5 |
| T26785 | 928-930 | NN | denotes | µg |
| T26786 | 930-931 | -RRB- | denotes | ) |
| T26787 | 932-934 | IN | denotes | in |
| T26788 | 935-938 | DT | denotes | the |
| T26789 | 939-947 | NN | denotes | presence |
| T26790 | 948-950 | CC | denotes | or |
| T26791 | 951-958 | NN | denotes | absence |
| T26792 | 959-961 | IN | denotes | of |
| T26793 | 962-969 | NNP | denotes | pRc/CMV |
| T26794 | 969-971 | CD | denotes | -3 |
| T26795 | 971-978 | NNP | denotes | xHA-p65 |
| T26796 | 978-979 | , | denotes | , |
| T26797 | 980-987 | NNP | denotes | pRc/CMV |
| T26798 | 987-989 | CD | denotes | -3 |
| T26799 | 989-998 | NN | denotes | xHA-p65ΔC |
| T26800 | 999-1000 | -LRB- | denotes | ( |
| T26801 | 1000-1001 | CD | denotes | 1 |
| T26802 | 1002-1005 | CD | denotes | 318 |
| T26803 | 1005-1006 | -RRB- | denotes | ) |
| T26804 | 1006-1007 | , | denotes | , |
| T26805 | 1008-1010 | CC | denotes | or |
| T26806 | 1011-1018 | NNP | denotes | pRc/CMV |
| T26807 | 1018-1020 | CD | denotes | -3 |
| T26808 | 1020-1029 | NNP | denotes | xHA-p65ΔN |
| T26809 | 1030-1031 | -LRB- | denotes | ( |
| T26810 | 1031-1034 | CD | denotes | 122 |
| T26811 | 1035-1038 | CD | denotes | 551 |
| T26812 | 1038-1039 | -RRB- | denotes | ) |
| T26813 | 1040-1041 | -LRB- | denotes | ( |
| T26814 | 1041-1042 | CD | denotes | 5 |
| T26815 | 1043-1045 | NN | denotes | µg |
| T26816 | 1045-1046 | -RRB- | denotes | ) |
| T26817 | 1046-1047 | . | denotes | . |
| T26818 | 1048-1057 | JJ | denotes | Wild-type |
| T26819 | 1058-1061 | CC | denotes | and |
| T26820 | 1062-1068 | JJ | denotes | mutant |
| T26821 | 1069-1075 | NN | denotes | HA-p65 |
| T26822 | 1076-1084 | NNS | denotes | proteins |
| T26823 | 1085-1089 | VBD | denotes | were |
| T26824 | 1090-1108 | VBN | denotes | immunoprecipitated |
| T26825 | 1109-1113 | IN | denotes | with |
| T26826 | 1114-1121 | JJ | denotes | anti-HA |
| T26827 | 1122-1130 | NN | denotes | antibody |
| T26828 | 1130-1131 | : | denotes | ; |
| T26829 | 1132-1147 | NNS | denotes | immunocomplexes |
| T26830 | 1148-1152 | VBD | denotes | were |
| T26831 | 1153-1162 | VBN | denotes | separated |
| T26832 | 1163-1165 | IN | denotes | by |
| T26833 | 1166-1168 | CD | denotes | 12 |
| T26834 | 1168-1169 | NN | denotes | % |
| T26835 | 1170-1173 | NNP | denotes | SDS |
| T26836 | 1174-1178 | NNP | denotes | PAGE |
| T26837 | 1179-1182 | CC | denotes | and |
| T26838 | 1183-1191 | VBN | denotes | analysed |
| T26839 | 1192-1194 | IN | denotes | by |
| T26840 | 1195-1202 | JJ | denotes | western |
| T26841 | 1203-1211 | VBG | denotes | blotting |
| T26842 | 1212-1216 | IN | denotes | with |
| T26843 | 1217-1226 | JJ | denotes | anti-FLAG |
| T26844 | 1227-1229 | CC | denotes | or |
| T26845 | 1230-1237 | JJ | denotes | anti-HA |
| T26846 | 1238-1248 | NNS | denotes | antibodies |
| T26847 | 1248-1249 | . | denotes | . |
| T26848 | 1250-1251 | -LRB- | denotes | ( |
| T26849 | 1251-1252 | NNP | denotes | E |
| T26850 | 1252-1253 | -RRB- | denotes | ) |
| T26851 | 1254-1265 | JJ | denotes | Recombinant |
| T26852 | 1266-1269 | NNS | denotes | p65 |
| T26853 | 1270-1277 | NN | denotes | protein |
| T26854 | 1278-1279 | -LRB- | denotes | ( |
| T26855 | 1279-1282 | CD | denotes | 100 |
| T26856 | 1283-1285 | NN | denotes | ng |
| T26857 | 1285-1286 | : | denotes | ; |
| T26858 | 1287-1293 | NNP | denotes | Active |
| T26859 | 1294-1299 | NNP | denotes | Motif |
| T26860 | 1300-1308 | NNP | denotes | Carlsbad |
| T26861 | 1308-1309 | , | denotes | , |
| T26862 | 1310-1312 | NNP | denotes | CA |
| T26863 | 1312-1313 | , | denotes | , |
| T26864 | 1314-1317 | NNP | denotes | USA |
| T26865 | 1317-1318 | -RRB- | denotes | ) |
| T26866 | 1319-1322 | VBD | denotes | was |
| T26867 | 1323-1325 | CD | denotes | 20 |
| T26868 | 1326-1329 | NN | denotes | min |
| T26869 | 1330-1339 | VBN | denotes | incubated |
| T26870 | 1340-1344 | IN | denotes | with |
| T26871 | 1345-1356 | JJ | denotes | 32P-labeled |
| T26872 | 1357-1362 | JJ | denotes | NF-κB |
| T26873 | 1363-1378 | JJ | denotes | double-stranded |
| T26874 | 1379-1394 | NN | denotes | oligonucleotide |
| T26875 | 1395-1397 | IN | denotes | in |
| T26876 | 1398-1406 | NN | denotes | presence |
| T26877 | 1407-1409 | CC | denotes | or |
| T26878 | 1410-1417 | NN | denotes | absence |
| T26879 | 1418-1420 | IN | denotes | of |
| T26880 | 1421-1423 | IN | denotes | in |
| T26881 | 1424-1429 | NN | denotes | vitro |
| T26882 | 1430-1440 | VBN | denotes | translated |
| T26883 | 1441-1449 | NNP | denotes | FLAG-Tat |
| T26884 | 1450-1452 | CC | denotes | or |
| T26885 | 1453-1461 | NNP | denotes | FLAG-Tat |
| T26886 | 1462-1463 | NNP | denotes | C |
| T26887 | 1463-1464 | -LRB- | denotes | ( |
| T26888 | 1464-1472 | CD | denotes | 22,25,27 |
| T26889 | 1472-1473 | -RRB- | denotes | ) |
| T26890 | 1473-1474 | DT | denotes | A |
| T26891 | 1475-1476 | -LRB- | denotes | ( |
| T26892 | 1476-1477 | CD | denotes | 5 |
| T26893 | 1478-1480 | NN | denotes | µl |
| T26894 | 1480-1481 | -RRB- | denotes | ) |
| T26895 | 1481-1482 | : | denotes | ; |
| T26896 | 1483-1495 | NNS | denotes | competitions |
| T26897 | 1496-1500 | VBD | denotes | were |
| T26898 | 1501-1510 | VBN | denotes | performed |
| T26899 | 1511-1515 | IN | denotes | with |
| T26900 | 1516-1520 | CD | denotes | 1.25 |
| T26901 | 1520-1521 | : | denotes | - |
| T26902 | 1522-1524 | RP | denotes | up |
| T26903 | 1525-1527 | TO | denotes | to |
| T26904 | 1528-1535 | JJ | denotes | 40-fold |
| T26905 | 1536-1541 | NN | denotes | molar |
| T26906 | 1542-1548 | NN | denotes | excess |
| T26907 | 1549-1551 | IN | denotes | of |
| T26908 | 1552-1561 | JJ | denotes | unlabeled |
| T26909 | 1562-1577 | NN | denotes | oligonucleotide |
| T26910 | 1577-1578 | . | denotes | . |
| T26911 | 1579-1590 | NNP | denotes | DNA/protein |
| T26912 | 1591-1600 | NNS | denotes | complexes |
| T26913 | 1601-1605 | VBD | denotes | were |
| T26914 | 1606-1615 | VBN | denotes | separated |
| T26915 | 1616-1618 | IN | denotes | by |
| T26916 | 1619-1620 | CD | denotes | 6 |
| T26917 | 1620-1621 | NN | denotes | % |
| T26918 | 1622-1626 | NN | denotes | PAGE |
| T26919 | 1627-1629 | IN | denotes | in |
| T26920 | 1630-1633 | CD | denotes | 0.5 |
| T26921 | 1634-1635 | CD | denotes | × |
| T26922 | 1636-1639 | NNP | denotes | TBE |
| T26923 | 1640-1646 | NN | denotes | buffer |
| T26924 | 1647-1650 | CC | denotes | and |
| T26925 | 1651-1659 | VBN | denotes | analysed |
| T26926 | 1660-1662 | IN | denotes | by |
| T26927 | 1663-1678 | NN | denotes | autoradiography |
| T26928 | 1678-1679 | . | denotes | . |
| T26929 | 1680-1681 | -LRB- | denotes | ( |
| T26930 | 1681-1682 | NN | denotes | F |
| T26931 | 1682-1683 | -RRB- | denotes | ) |
| T26932 | 1684-1696 | NN | denotes | Densitometry |
| T26933 | 1697-1699 | IN | denotes | of |
| T26934 | 1700-1711 | NNS | denotes | band-shifts |
| T26935 | 1712-1717 | VBN | denotes | shown |
| T26936 | 1718-1720 | IN | denotes | in |
| T26937 | 1721-1723 | NNP | denotes | E. |
| R20925 | T26585 | T26586 | compound | Tat,associates |
| R20926 | T26586 | T26586 | ROOT | associates,associates |
| R20927 | T26587 | T26586 | prep | with,associates |
| R20928 | T26588 | T26589 | nsubj | p65,increasing |
| R20929 | T26589 | T26587 | pcomp | increasing,with |
| R20930 | T26590 | T26592 | poss | its,binding |
| R20931 | T26591 | T26592 | advmod | DNA-affinity,binding |
| R20932 | T26592 | T26589 | dobj | binding,increasing |
| R20933 | T26593 | T26586 | punct | .,associates |
| R20934 | T26594 | T26600 | punct | (,transfected |
| R20935 | T26595 | T26598 | nmod | A,cells |
| R20936 | T26596 | T26595 | punct | ),A |
| R20937 | T26597 | T26598 | compound | HeLa,cells |
| R20938 | T26598 | T26600 | nsubjpass | cells,transfected |
| R20939 | T26599 | T26600 | auxpass | were,transfected |
| R20940 | T26600 | T26600 | ROOT | transfected,transfected |
| R20941 | T26601 | T26600 | prep | with,transfected |
| R20942 | T26602 | T26601 | pobj | pRc/CMV,with |
| R20943 | T26603 | T26604 | nummod | -3,xHA-p65 |
| R20944 | T26604 | T26600 | npadvmod | xHA-p65,transfected |
| R20945 | T26605 | T26607 | punct | (,µg |
| R20946 | T26606 | T26607 | nummod | 5,µg |
| R20947 | T26607 | T26604 | appos | µg,xHA-p65 |
| R20948 | T26608 | T26607 | punct | ),µg |
| R20949 | T26609 | T26604 | prep | in,xHA-p65 |
| R20950 | T26610 | T26609 | pobj | presence,in |
| R20951 | T26611 | T26610 | cc | or,presence |
| R20952 | T26612 | T26610 | conj | absence,presence |
| R20953 | T26613 | T26612 | prep | of,absence |
| R20954 | T26614 | T26613 | pobj | p3xFLAG-Tat,of |
| R20955 | T26615 | T26612 | punct | ",",absence |
| R20956 | T26616 | T26617 | compound | p3xFLAG-Tat,T |
| R20957 | T26617 | T26604 | conj | T,xHA-p65 |
| R20958 | T26618 | T26617 | punct | ",",T |
| R20959 | T26619 | T26617 | conj | N,T |
| R20960 | T26620 | T26621 | punct | (,"23,24" |
| R20961 | T26621 | T26619 | parataxis | "23,24",N |
| R20962 | T26622 | T26621 | punct | ),"23,24" |
| R20963 | T26623 | T26626 | det | A,K |
| R20964 | T26624 | T26626 | punct | ",",K |
| R20965 | T26625 | T26626 | compound | p3xFLAG-Tat,K |
| R20966 | T26626 | T26604 | npadvmod | K,xHA-p65 |
| R20967 | T26627 | T26626 | punct | (,K |
| R20968 | T26628 | T26626 | appos | "50,51",K |
| R20969 | T26629 | T26626 | punct | ),K |
| R20970 | T26630 | T26633 | det | A,R |
| R20971 | T26631 | T26633 | punct | ",",R |
| R20972 | T26632 | T26633 | amod | p3xFLAG-Tat,R |
| R20973 | T26633 | T26633 | ROOT | R,R |
| R20974 | T26634 | T26633 | punct | (,R |
| R20975 | T26635 | T26633 | appos | 49,R |
| R20976 | T26636 | T26633 | appos | 57,R |
| R20977 | T26637 | T26633 | punct | ),R |
| R20978 | T26638 | T26638 | ROOT | A,A |
| R20979 | T26639 | T26638 | punct | ",",A |
| R20980 | T26640 | T26638 | cc | or,A |
| R20981 | T26641 | T26642 | compound | p3xFLAG-Tat,C |
| R20982 | T26642 | T26638 | conj | C,A |
| R20983 | T26643 | T26642 | punct | (,C |
| R20984 | T26644 | T26642 | appos | "22,25,27",C |
| R20985 | T26645 | T26642 | punct | ),C |
| R20986 | T26646 | T26649 | det | A,µg |
| R20987 | T26647 | T26649 | punct | (,µg |
| R20988 | T26648 | T26649 | nummod | 5,µg |
| R20989 | T26649 | T26649 | ROOT | µg,µg |
| R20990 | T26650 | T26649 | punct | ),µg |
| R20991 | T26651 | T26649 | punct | .,µg |
| R20992 | T26652 | T26653 | amod | FLAG-Tat,proteins |
| R20993 | T26653 | T26655 | nsubjpass | proteins,immunoprecipitated |
| R20994 | T26654 | T26655 | auxpass | were,immunoprecipitated |
| R20995 | T26655 | T26662 | ccomp | immunoprecipitated,separated |
| R20996 | T26656 | T26655 | prep | with,immunoprecipitated |
| R20997 | T26657 | T26658 | amod | anti-FLAG,antibody |
| R20998 | T26658 | T26656 | pobj | antibody,with |
| R20999 | T26659 | T26662 | punct | ;,separated |
| R21000 | T26660 | T26662 | nsubjpass | immunocomplexes,separated |
| R21001 | T26661 | T26662 | auxpass | were,separated |
| R21002 | T26662 | T26662 | ROOT | separated,separated |
| R21003 | T26663 | T26662 | prep | by,separated |
| R21004 | T26664 | T26665 | nummod | 12,% |
| R21005 | T26665 | T26666 | compound | %,SDS |
| R21006 | T26666 | T26663 | pobj | SDS,by |
| R21007 | T26667 | T26666 | conj | PAGE,SDS |
| R21008 | T26668 | T26662 | cc | and,separated |
| R21009 | T26669 | T26662 | conj | analysed,separated |
| R21010 | T26670 | T26669 | agent | by,analysed |
| R21011 | T26671 | T26672 | amod | western,blotting |
| R21012 | T26672 | T26670 | pobj | blotting,by |
| R21013 | T26673 | T26672 | prep | with,blotting |
| R21014 | T26674 | T26677 | amod | anti-FLAG,antibodies |
| R21015 | T26675 | T26674 | cc | or,anti-FLAG |
| R21016 | T26676 | T26674 | conj | anti-HA,anti-FLAG |
| R21017 | T26677 | T26673 | pobj | antibodies,with |
| R21018 | T26678 | T26662 | punct | .,separated |
| R21019 | T26679 | T26680 | punct | (,B |
| R21020 | T26680 | T26680 | ROOT | B,B |
| R21021 | T26681 | T26680 | punct | ),B |
| R21022 | T26682 | T26691 | prep | In,incubated |
| R21023 | T26683 | T26685 | amod | vitro,p65 |
| R21024 | T26684 | T26685 | amod | translated,p65 |
| R21025 | T26685 | T26682 | pobj | p65,In |
| R21026 | T26686 | T26685 | punct | (,p65 |
| R21027 | T26687 | T26688 | nummod | 5,µl |
| R21028 | T26688 | T26685 | appos | µl,p65 |
| R21029 | T26689 | T26688 | punct | ),µl |
| R21030 | T26690 | T26691 | auxpass | was,incubated |
| R21031 | T26691 | T26691 | ROOT | incubated,incubated |
| R21032 | T26692 | T26691 | prep | with,incubated |
| R21033 | T26693 | T26692 | pobj | GST-Tat,with |
| R21034 | T26694 | T26693 | punct | ",",GST-Tat |
| R21035 | T26695 | T26696 | compound | GST-Tat,T |
| R21036 | T26696 | T26693 | conj | T,GST-Tat |
| R21037 | T26697 | T26696 | punct | ",",T |
| R21038 | T26698 | T26696 | conj | N,T |
| R21039 | T26699 | T26700 | punct | (,"23,24" |
| R21040 | T26700 | T26698 | parataxis | "23,24",N |
| R21041 | T26701 | T26700 | punct | ),"23,24" |
| R21042 | T26702 | T26705 | det | A,K |
| R21043 | T26703 | T26705 | punct | ",",K |
| R21044 | T26704 | T26705 | compound | GST-Tat,K |
| R21045 | T26705 | T26705 | ROOT | K,K |
| R21046 | T26706 | T26705 | punct | (,K |
| R21047 | T26707 | T26705 | appos | "50,51",K |
| R21048 | T26708 | T26705 | punct | ),K |
| R21049 | T26709 | T26712 | det | A,R |
| R21050 | T26710 | T26712 | punct | ",",R |
| R21051 | T26711 | T26712 | compound | GST-Tat,R |
| R21052 | T26712 | T26712 | ROOT | R,R |
| R21053 | T26713 | T26712 | punct | (,R |
| R21054 | T26714 | T26712 | appos | 49,R |
| R21055 | T26715 | T26712 | appos | 57,R |
| R21056 | T26716 | T26712 | punct | ),R |
| R21057 | T26717 | T26720 | det | A,C |
| R21058 | T26718 | T26720 | punct | ",",C |
| R21059 | T26719 | T26720 | compound | GST-Tat,C |
| R21060 | T26720 | T26720 | ROOT | C,C |
| R21061 | T26721 | T26720 | punct | (,C |
| R21062 | T26722 | T26720 | appos | "22,25,27",C |
| R21063 | T26723 | T26720 | punct | ),C |
| R21064 | T26724 | T26724 | ROOT | A,A |
| R21065 | T26725 | T26724 | punct | ",",A |
| R21066 | T26726 | T26724 | cc | or,A |
| R21067 | T26727 | T26724 | conj | GST,A |
| R21068 | T26728 | T26730 | punct | (,µg |
| R21069 | T26729 | T26730 | nummod | 5,µg |
| R21070 | T26730 | T26727 | appos | µg,GST |
| R21071 | T26731 | T26730 | punct | ),µg |
| R21072 | T26732 | T26727 | amod | conjugated,GST |
| R21073 | T26733 | T26732 | prep | with,conjugated |
| R21074 | T26734 | T26733 | pobj | Glutathione-Sepharose,with |
| R21075 | T26735 | T26727 | punct | .,GST |
| R21076 | T26736 | T26737 | compound | Protein,complexes |
| R21077 | T26737 | T26739 | nsubjpass | complexes,recovered |
| R21078 | T26738 | T26739 | auxpass | were,recovered |
| R21079 | T26739 | T26739 | ROOT | recovered,recovered |
| R21080 | T26740 | T26739 | agent | by,recovered |
| R21081 | T26741 | T26740 | pobj | GST-pull,by |
| R21082 | T26742 | T26739 | advmod | down,recovered |
| R21083 | T26743 | T26739 | punct | ",",recovered |
| R21084 | T26744 | T26739 | conj | separated,recovered |
| R21085 | T26745 | T26744 | prep | by,separated |
| R21086 | T26746 | T26747 | nummod | 12,% |
| R21087 | T26747 | T26748 | compound | %,SDS |
| R21088 | T26748 | T26745 | pobj | SDS,by |
| R21089 | T26749 | T26748 | conj | PAGE,SDS |
| R21090 | T26750 | T26748 | punct | ",",SDS |
| R21091 | T26751 | T26744 | cc | and,separated |
| R21092 | T26752 | T26744 | conj | analysed,separated |
| R21093 | T26753 | T26752 | agent | by,analysed |
| R21094 | T26754 | T26755 | amod | western,blotting |
| R21095 | T26755 | T26753 | pobj | blotting,by |
| R21096 | T26756 | T26755 | prep | with,blotting |
| R21097 | T26757 | T26760 | amod | anti-p65,antibodies |
| R21098 | T26758 | T26757 | cc | or,anti-p65 |
| R21099 | T26759 | T26757 | conj | anti-GST,anti-p65 |
| R21100 | T26760 | T26756 | pobj | antibodies,with |
| R21101 | T26761 | T26739 | punct | .,recovered |
| R21102 | T26762 | T26763 | punct | (,C |
| R21103 | T26763 | T26766 | nmod | C,representation |
| R21104 | T26764 | T26763 | punct | ),C |
| R21105 | T26765 | T26766 | amod | Schematic,representation |
| R21106 | T26766 | T26766 | ROOT | representation,representation |
| R21107 | T26767 | T26766 | prep | of,representation |
| R21108 | T26768 | T26772 | amod | wild-type,proteins |
| R21109 | T26769 | T26768 | cc | and,wild-type |
| R21110 | T26770 | T26768 | conj | mutant,wild-type |
| R21111 | T26771 | T26772 | compound | p65,proteins |
| R21112 | T26772 | T26767 | pobj | proteins,of |
| R21113 | T26773 | T26766 | punct | .,representation |
| R21114 | T26774 | T26780 | punct | (,transfected |
| R21115 | T26775 | T26778 | compound | D,cells |
| R21116 | T26776 | T26777 | punct | ),HeLa |
| R21117 | T26777 | T26778 | compound | HeLa,cells |
| R21118 | T26778 | T26780 | nsubjpass | cells,transfected |
| R21119 | T26779 | T26780 | auxpass | were,transfected |
| R21120 | T26780 | T26780 | ROOT | transfected,transfected |
| R21121 | T26781 | T26780 | prep | with,transfected |
| R21122 | T26782 | T26781 | pobj | p3xFLAG-Tat,with |
| R21123 | T26783 | T26785 | punct | (,µg |
| R21124 | T26784 | T26785 | nummod | 5,µg |
| R21125 | T26785 | T26782 | appos | µg,p3xFLAG-Tat |
| R21126 | T26786 | T26785 | punct | ),µg |
| R21127 | T26787 | T26780 | prep | in,transfected |
| R21128 | T26788 | T26789 | det | the,presence |
| R21129 | T26789 | T26787 | pobj | presence,in |
| R21130 | T26790 | T26789 | cc | or,presence |
| R21131 | T26791 | T26789 | conj | absence,presence |
| R21132 | T26792 | T26791 | prep | of,absence |
| R21133 | T26793 | T26795 | nmod | pRc/CMV,xHA-p65 |
| R21134 | T26794 | T26795 | nummod | -3,xHA-p65 |
| R21135 | T26795 | T26792 | pobj | xHA-p65,of |
| R21136 | T26796 | T26795 | punct | ",",xHA-p65 |
| R21137 | T26797 | T26799 | nmod | pRc/CMV,xHA-p65ΔC |
| R21138 | T26798 | T26799 | nummod | -3,xHA-p65ΔC |
| R21139 | T26799 | T26795 | appos | xHA-p65ΔC,xHA-p65 |
| R21140 | T26800 | T26801 | punct | (,1 |
| R21141 | T26801 | T26808 | nummod | 1,xHA-p65ΔN |
| R21142 | T26802 | T26801 | appos | 318,1 |
| R21143 | T26803 | T26801 | punct | ),1 |
| R21144 | T26804 | T26801 | punct | ",",1 |
| R21145 | T26805 | T26801 | cc | or,1 |
| R21146 | T26806 | T26808 | nmod | pRc/CMV,xHA-p65ΔN |
| R21147 | T26807 | T26808 | nummod | -3,xHA-p65ΔN |
| R21148 | T26808 | T26799 | appos | xHA-p65ΔN,xHA-p65ΔC |
| R21149 | T26809 | T26808 | punct | (,xHA-p65ΔN |
| R21150 | T26810 | T26811 | dep | 122,551 |
| R21151 | T26811 | T26808 | appos | 551,xHA-p65ΔN |
| R21152 | T26812 | T26808 | punct | ),xHA-p65ΔN |
| R21153 | T26813 | T26815 | punct | (,µg |
| R21154 | T26814 | T26815 | nummod | 5,µg |
| R21155 | T26815 | T26808 | appos | µg,xHA-p65ΔN |
| R21156 | T26816 | T26815 | punct | ),µg |
| R21157 | T26817 | T26780 | punct | .,transfected |
| R21158 | T26818 | T26822 | amod | Wild-type,proteins |
| R21159 | T26819 | T26818 | cc | and,Wild-type |
| R21160 | T26820 | T26818 | conj | mutant,Wild-type |
| R21161 | T26821 | T26822 | compound | HA-p65,proteins |
| R21162 | T26822 | T26824 | nsubjpass | proteins,immunoprecipitated |
| R21163 | T26823 | T26824 | auxpass | were,immunoprecipitated |
| R21164 | T26824 | T26831 | ccomp | immunoprecipitated,separated |
| R21165 | T26825 | T26824 | prep | with,immunoprecipitated |
| R21166 | T26826 | T26827 | amod | anti-HA,antibody |
| R21167 | T26827 | T26825 | pobj | antibody,with |
| R21168 | T26828 | T26831 | punct | ;,separated |
| R21169 | T26829 | T26831 | nsubjpass | immunocomplexes,separated |
| R21170 | T26830 | T26831 | auxpass | were,separated |
| R21171 | T26831 | T26831 | ROOT | separated,separated |
| R21172 | T26832 | T26831 | prep | by,separated |
| R21173 | T26833 | T26834 | nummod | 12,% |
| R21174 | T26834 | T26832 | pobj | %,by |
| R21175 | T26835 | T26836 | compound | SDS,PAGE |
| R21176 | T26836 | T26831 | npadvmod | PAGE,separated |
| R21177 | T26837 | T26836 | cc | and,PAGE |
| R21178 | T26838 | T26836 | conj | analysed,PAGE |
| R21179 | T26839 | T26838 | agent | by,analysed |
| R21180 | T26840 | T26841 | amod | western,blotting |
| R21181 | T26841 | T26839 | pobj | blotting,by |
| R21182 | T26842 | T26841 | prep | with,blotting |
| R21183 | T26843 | T26846 | amod | anti-FLAG,antibodies |
| R21184 | T26844 | T26843 | cc | or,anti-FLAG |
| R21185 | T26845 | T26843 | conj | anti-HA,anti-FLAG |
| R21186 | T26846 | T26842 | pobj | antibodies,with |
| R21187 | T26847 | T26831 | punct | .,separated |
| R21188 | T26848 | T26866 | punct | (,was |
| R21189 | T26849 | T26853 | nmod | E,protein |
| R21190 | T26850 | T26849 | punct | ),E |
| R21191 | T26851 | T26852 | amod | Recombinant,p65 |
| R21192 | T26852 | T26853 | compound | p65,protein |
| R21193 | T26853 | T26866 | ccomp | protein,was |
| R21194 | T26854 | T26856 | punct | (,ng |
| R21195 | T26855 | T26856 | nummod | 100,ng |
| R21196 | T26856 | T26853 | appos | ng,protein |
| R21197 | T26857 | T26866 | punct | ;,was |
| R21198 | T26858 | T26860 | compound | Active,Carlsbad |
| R21199 | T26859 | T26860 | compound | Motif,Carlsbad |
| R21200 | T26860 | T26866 | nsubj | Carlsbad,was |
| R21201 | T26861 | T26860 | punct | ",",Carlsbad |
| R21202 | T26862 | T26860 | conj | CA,Carlsbad |
| R21203 | T26863 | T26862 | punct | ",",CA |
| R21204 | T26864 | T26862 | conj | USA,CA |
| R21205 | T26865 | T26862 | punct | ),CA |
| R21206 | T26866 | T26866 | ROOT | was,was |
| R21207 | T26867 | T26868 | nummod | 20,min |
| R21208 | T26868 | T26866 | attr | min,was |
| R21209 | T26869 | T26868 | acl | incubated,min |
| R21210 | T26870 | T26869 | prep | with,incubated |
| R21211 | T26871 | T26874 | amod | 32P-labeled,oligonucleotide |
| R21212 | T26872 | T26874 | amod | NF-κB,oligonucleotide |
| R21213 | T26873 | T26874 | amod | double-stranded,oligonucleotide |
| R21214 | T26874 | T26870 | pobj | oligonucleotide,with |
| R21215 | T26875 | T26874 | prep | in,oligonucleotide |
| R21216 | T26876 | T26875 | pobj | presence,in |
| R21217 | T26877 | T26876 | cc | or,presence |
| R21218 | T26878 | T26876 | conj | absence,presence |
| R21219 | T26879 | T26876 | prep | of,presence |
| R21220 | T26880 | T26882 | mark | in,translated |
| R21221 | T26881 | T26882 | nsubj | vitro,translated |
| R21222 | T26882 | T26869 | advcl | translated,incubated |
| R21223 | T26883 | T26882 | dobj | FLAG-Tat,translated |
| R21224 | T26884 | T26883 | cc | or,FLAG-Tat |
| R21225 | T26885 | T26886 | compound | FLAG-Tat,C |
| R21226 | T26886 | T26883 | conj | C,FLAG-Tat |
| R21227 | T26887 | T26886 | punct | (,C |
| R21228 | T26888 | T26886 | appos | "22,25,27",C |
| R21229 | T26889 | T26886 | punct | ),C |
| R21230 | T26890 | T26893 | det | A,µl |
| R21231 | T26891 | T26893 | punct | (,µl |
| R21232 | T26892 | T26893 | nummod | 5,µl |
| R21233 | T26893 | T26893 | ROOT | µl,µl |
| R21234 | T26894 | T26893 | punct | ),µl |
| R21235 | T26895 | T26898 | punct | ;,performed |
| R21236 | T26896 | T26898 | nsubjpass | competitions,performed |
| R21237 | T26897 | T26898 | auxpass | were,performed |
| R21238 | T26898 | T26898 | ROOT | performed,performed |
| R21239 | T26899 | T26898 | prep | with,performed |
| R21240 | T26900 | T26906 | nummod | 1.25,excess |
| R21241 | T26901 | T26902 | punct | -,up |
| R21242 | T26902 | T26906 | advmod | up,excess |
| R21243 | T26903 | T26906 | quantmod | to,excess |
| R21244 | T26904 | T26903 | pobj | 40-fold,to |
| R21245 | T26905 | T26906 | compound | molar,excess |
| R21246 | T26906 | T26899 | pobj | excess,with |
| R21247 | T26907 | T26906 | prep | of,excess |
| R21248 | T26908 | T26909 | amod | unlabeled,oligonucleotide |
| R21249 | T26909 | T26907 | pobj | oligonucleotide,of |
| R21250 | T26910 | T26898 | punct | .,performed |
| R21251 | T26911 | T26912 | compound | DNA/protein,complexes |
| R21252 | T26912 | T26914 | nsubjpass | complexes,separated |
| R21253 | T26913 | T26914 | auxpass | were,separated |
| R21254 | T26914 | T26914 | ROOT | separated,separated |
| R21255 | T26915 | T26914 | prep | by,separated |
| R21256 | T26916 | T26917 | nummod | 6,% |
| R21257 | T26917 | T26918 | compound | %,PAGE |
| R21258 | T26918 | T26915 | pobj | PAGE,by |
| R21259 | T26919 | T26914 | prep | in,separated |
| R21260 | T26920 | T26923 | nummod | 0.5,buffer |
| R21261 | T26921 | T26923 | nummod | ×,buffer |
| R21262 | T26922 | T26923 | compound | TBE,buffer |
| R21263 | T26923 | T26919 | pobj | buffer,in |
| R21264 | T26924 | T26923 | cc | and,buffer |
| R21265 | T26925 | T26914 | advcl | analysed,separated |
| R21266 | T26926 | T26925 | agent | by,analysed |
| R21267 | T26927 | T26926 | pobj | autoradiography,by |
| R21268 | T26928 | T26914 | punct | .,separated |
| R21269 | T26929 | T26932 | punct | (,Densitometry |
| R21270 | T26930 | T26932 | nmod | F,Densitometry |
| R21271 | T26931 | T26932 | punct | ),Densitometry |
| R21272 | T26932 | T26932 | ROOT | Densitometry,Densitometry |
| R21273 | T26933 | T26932 | prep | of,Densitometry |
| R21274 | T26934 | T26933 | pobj | band-shifts,of |
| R21275 | T26935 | T26932 | acl | shown,Densitometry |
| R21276 | T26936 | T26935 | prep | in,shown |
| R21277 | T26937 | T26936 | pobj | E.,in |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26938 | 476-486 | http://purl.obolibrary.org/obo/GO_0006412 | denotes | translated |
| T26939 | 1430-1440 | http://purl.obolibrary.org/obo/GO_0006412 | denotes | translated |
| T26940 | 1310-1312 | http://purl.obolibrary.org/obo/GO_0033968 | denotes | CA |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26941 | 62-69 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
| T26942 | 335-343 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
| T26943 | 1122-1130 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
| T26944 | 1238-1248 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibodies |
| T26945 | 1310-1312 | http://purl.obolibrary.org/obo/GO_0033968 | denotes | CA |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26946 | 80-85 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T26947 | 885-890 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T26948 | 335-343 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
| T26949 | 1122-1130 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
| T26950 | 1238-1248 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibodies |
| T26951 | 335-343 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
| T26952 | 1122-1130 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
| T26953 | 1238-1248 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibodies |
| T26954 | 660-677 | http://purl.obolibrary.org/obo/GO_0043234 | denotes | Protein complexes |
| T26955 | 1583-1600 | http://purl.obolibrary.org/obo/GO_0043234 | denotes | protein complexes |
| T26956 | 1579-1600 | http://purl.obolibrary.org/obo/GO_0001114 | denotes | DNA/protein complexes |
| T26957 | 1579-1600 | http://purl.obolibrary.org/obo/GO_0032993 | denotes | DNA/protein complexes |
| T26958 | 1579-1600 | http://purl.obolibrary.org/obo/GO_0097522 | denotes | DNA/protein complexes |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26499 | 10-277 | Sentence | denotes | Tat associates with p65 increasing its DNA-affinity binding. (A) HeLa cells were transfected with pRc/CMV-3xHA-p65 (5 µg) in presence or absence of p3xFLAG-Tat, p3xFLAG-Tat T,N(23,24)A, p3xFLAG-Tat K(50,51)A, p3xFLAG-Tat R(49–57)A, or p3xFLAG-Tat C(22,25,27)A (5 µg). |
| T26500 | 278-1047 | Sentence | denotes | FLAG-Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (B) In vitro translated p65 (5 µl) was incubated with GST-Tat, GST-Tat T,N(23,24)A, GST-Tat K(50,51)A, GST-Tat R(49–57)A, GST-Tat C(22,25,27)A, or GST (5 µg) conjugated with Glutathione-Sepharose. Protein complexes were recovered by GST-pull down, separated by 12% SDS–PAGE, and analysed by western blotting with anti-p65 or anti-GST antibodies. (C) Schematic representation of wild-type and mutant p65 proteins. (D) HeLa cells were transfected with p3xFLAG-Tat (5 µg) in the presence or absence of pRc/CMV-3xHA-p65, pRc/CMV-3xHA-p65ΔC (1–318), or pRc/CMV-3xHA-p65ΔN (122–551) (5 µg). |
| T26501 | 1048-1578 | Sentence | denotes | Wild-type and mutant HA-p65 proteins were immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (E) Recombinant p65 protein (100 ng; Active Motif Carlsbad, CA, USA) was 20 min incubated with 32P-labeled NF-κB double-stranded oligonucleotide in presence or absence of in vitro translated FLAG-Tat or FLAG-Tat C(22,25,27)A (5 µl); competitions were performed with 1.25- up to 40-fold molar excess of unlabeled oligonucleotide. |
| T26502 | 1579-1723 | Sentence | denotes | DNA/protein complexes were separated by 6% PAGE in 0.5 × TBE buffer and analysed by autoradiography. (F) Densitometry of band-shifts shown in E. |
| T171 | 0-9 | Sentence | denotes | Figure 4. |
| T172 | 10-277 | Sentence | denotes | Tat associates with p65 increasing its DNA-affinity binding. (A) HeLa cells were transfected with pRc/CMV-3xHA-p65 (5 µg) in presence or absence of p3xFLAG-Tat, p3xFLAG-Tat T,N(23,24)A, p3xFLAG-Tat K(50,51)A, p3xFLAG-Tat R(49–57)A, or p3xFLAG-Tat C(22,25,27)A (5 µg). |
| T173 | 278-659 | Sentence | denotes | FLAG-Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (B) In vitro translated p65 (5 µl) was incubated with GST-Tat, GST-Tat T,N(23,24)A, GST-Tat K(50,51)A, GST-Tat R(49–57)A, GST-Tat C(22,25,27)A, or GST (5 µg) conjugated with Glutathione-Sepharose. |
| T174 | 660-1047 | Sentence | denotes | Protein complexes were recovered by GST-pull down, separated by 12% SDS–PAGE, and analysed by western blotting with anti-p65 or anti-GST antibodies. (C) Schematic representation of wild-type and mutant p65 proteins. (D) HeLa cells were transfected with p3xFLAG-Tat (5 µg) in the presence or absence of pRc/CMV-3xHA-p65, pRc/CMV-3xHA-p65ΔC (1–318), or pRc/CMV-3xHA-p65ΔN (122–551) (5 µg). |
| T175 | 1048-1578 | Sentence | denotes | Wild-type and mutant HA-p65 proteins were immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (E) Recombinant p65 protein (100 ng; Active Motif Carlsbad, CA, USA) was 20 min incubated with 32P-labeled NF-κB double-stranded oligonucleotide in presence or absence of in vitro translated FLAG-Tat or FLAG-Tat C(22,25,27)A (5 µl); competitions were performed with 1.25- up to 40-fold molar excess of unlabeled oligonucleotide. |
| T176 | 1579-1723 | Sentence | denotes | DNA/protein complexes were separated by 6% PAGE in 0.5 × TBE buffer and analysed by autoradiography. (F) Densitometry of band-shifts shown in E. |
events-check-again
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T27104 | 10-13 | Protein | denotes | Tat |
| T27105 | 14-24 | Binding | denotes | associates |
| T27106 | 30-33 | Protein | denotes | p65 |
| T27107 | 34-44 | Positive_regulation | denotes | increasing |
| T27108 | 62-69 | Binding | denotes | binding |
| T27109 | 116-124 | Protein | denotes | 3xHA-p65 |
| T27110 | 166-169 | Protein | denotes | Tat |
| T27111 | 179-194 | Protein | denotes | Tat T,N(23,24)A |
| T27112 | 204-217 | Protein | denotes | Tat K(50,51)A |
| T27113 | 227-240 | Protein | denotes | Tat R(49–57)A |
| T27114 | 253-269 | Protein | denotes | Tat C(22,25,27)A |
| T27115 | 278-286 | Protein | denotes | FLAG-Tat |
| T27116 | 487-490 | Protein | denotes | p65 |
| T27117 | 517-524 | Protein | denotes | GST-Tat |
| T27118 | 526-545 | Protein | denotes | GST-Tat T,N(23,24)A |
| T27119 | 547-564 | Protein | denotes | GST-Tat K(50,51)A |
| T27120 | 566-583 | Protein | denotes | GST-Tat R(49–57)A |
| T27121 | 585-605 | Protein | denotes | GST-Tat C(22,25,27)A |
| T27122 | 610-613 | Protein | denotes | GST |
| T27123 | 696-699 | Protein | denotes | GST |
| T27124 | 862-865 | Protein | denotes | p65 |
| T27125 | 921-924 | Protein | denotes | Tat |
| T27126 | 970-978 | Protein | denotes | 3xHA-p65 |
| T27127 | 988-998 | Protein | denotes | 3xHA-p65ΔC |
| T27128 | 1019-1029 | Protein | denotes | 3xHA-p65ΔN |
| T27129 | 1069-1075 | Protein | denotes | HA-p65 |
| T27130 | 1266-1269 | Protein | denotes | p65 |
| T27131 | 1441-1449 | Protein | denotes | FLAG-Tat |
| T27132 | 1453-1474 | Protein | denotes | FLAG-Tat C(22,25,27)A |
| R21302 | T27104 | T27105 | themeOf | Tat,associates |
| R21303 | T27105 | T27107 | causeOf | associates,increasing |
| R21304 | T27106 | T27105 | themeOf | p65,associates |
| R21305 | T27106 | T27108 | themeOf | p65,binding |
| R21306 | T27108 | T27107 | themeOf | binding,increasing |
bionlp-st-ge-2016-reference-tees
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T27133 | 10-13 | Protein | denotes | Tat |
| T27134 | 30-33 | Protein | denotes | p65 |
| T27135 | 14-24 | Binding | denotes | associates |
| T27136 | 158-169 | Protein | denotes | p3xFLAG-Tat |
| T27137 | 171-178 | Protein | denotes | p3xFLAG |
| T27138 | 179-182 | Protein | denotes | Tat |
| T27139 | 278-295 | Protein | denotes | FLAG-Tat proteins |
| T27140 | 517-520 | Protein | denotes | GST |
| T27141 | 521-524 | Protein | denotes | Tat |
| T27142 | 526-529 | Protein | denotes | GST |
| T27143 | 530-533 | Protein | denotes | Tat |
| T27144 | 566-569 | Protein | denotes | GST |
| T27145 | 570-573 | Protein | denotes | Tat |
| T27146 | 696-699 | Protein | denotes | GST |
| T27147 | 781-784 | Protein | denotes | p65 |
| T27148 | 788-807 | Protein | denotes | anti-GST antibodies |
| T27149 | 855-874 | Protein | denotes | mutant p65 proteins |
| T27150 | 1062-1084 | Protein | denotes | mutant HA-p65 proteins |
| T27151 | 1114-1130 | Protein | denotes | anti-HA antibody |
| T27152 | 1090-1108 | Binding | denotes | immunoprecipitated |
| T27153 | 1090-1108 | Binding | denotes | immunoprecipitated |
| T27154 | 1254-1277 | Protein | denotes | Recombinant p65 protein |
| T27155 | 1357-1362 | Protein | denotes | NF-κB |
| T27156 | 1441-1445 | Protein | denotes | FLAG |
| T27157 | 1446-1449 | Protein | denotes | Tat |
| T27158 | 1453-1457 | Protein | denotes | FLAG |
| T27159 | 1458-1461 | Protein | denotes | Tat |
| R21307 | T27133 | T27135 | themeOf | Tat,associates |
| R21308 | T27150 | T27153 | themeOf | mutant HA-p65 proteins,immunoprecipitated |
| R21309 | T27151 | T27152 | themeOf | anti-HA antibody,immunoprecipitated |
| R21310 | T27151 | T27153 | themeOf | anti-HA antibody,immunoprecipitated |
test2
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26440 | 10-13 | Protein | denotes | Tat |
| T26441 | 14-24 | Binding | denotes | associates |
| T26442 | 30-33 | Protein | denotes | p65 |
| T26443 | 34-44 | Positive_regulation | denotes | increasing |
| T26444 | 62-69 | Binding | denotes | binding |
| T26445 | 116-124 | Protein | denotes | 3xHA-p65 |
| T26446 | 147-154 | Negative_regulation | denotes | absence |
| T26447 | 166-169 | Protein | denotes | Tat |
| T26448 | 179-194 | Protein | denotes | Tat T,N(23,24)A |
| T26449 | 204-217 | Protein | denotes | Tat K(50,51)A |
| T26450 | 227-240 | Protein | denotes | Tat R(49–57)A |
| T26451 | 253-269 | Protein | denotes | Tat C(22,25,27)A |
| T26452 | 278-286 | Protein | denotes | FLAG-Tat |
| T26453 | 487-490 | Protein | denotes | p65 |
| T26454 | 517-524 | Protein | denotes | GST-Tat |
| T26455 | 526-545 | Protein | denotes | GST-Tat T,N(23,24)A |
| T26456 | 547-564 | Protein | denotes | GST-Tat K(50,51)A |
| T26457 | 566-583 | Protein | denotes | GST-Tat R(49–57)A |
| T26458 | 585-605 | Protein | denotes | GST-Tat C(22,25,27)A |
| T26459 | 610-613 | Protein | denotes | GST |
| T26460 | 696-699 | Protein | denotes | GST |
| T26461 | 862-865 | Protein | denotes | p65 |
| T26462 | 921-924 | Protein | denotes | Tat |
| T26463 | 970-978 | Protein | denotes | 3xHA-p65 |
| T26464 | 988-998 | Protein | denotes | 3xHA-p65ΔC |
| T26465 | 1019-1029 | Protein | denotes | 3xHA-p65ΔN |
| T26466 | 1069-1075 | Protein | denotes | HA-p65 |
| T26467 | 1266-1269 | Protein | denotes | p65 |
| T26468 | 1441-1449 | Protein | denotes | FLAG-Tat |
| T26469 | 1453-1474 | Protein | denotes | FLAG-Tat C(22,25,27)A |
| R20914 | T26440 | T26441 | themeOf | Tat,associates |
| R20915 | T26440 | T26444 | themeOf | Tat,binding |
| R20916 | T26441 | T26443 | causeOf | associates,increasing |
| R20917 | T26442 | T26441 | themeOf | p65,associates |
| R20918 | T26444 | T26443 | themeOf | binding,increasing |
bionlp-st-ge-2016-reference
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26497 | 1441-1449 | Protein | denotes | FLAG-Tat |
| T26498 | 1453-1474 | Protein | denotes | FLAG-Tat C(22,25,27)A |
| T26470 | 10-13 | Protein | denotes | Tat |
| T26471 | 14-24 | Binding | denotes | associates |
| T26472 | 30-33 | Protein | denotes | p65 |
| T26473 | 34-44 | Positive_regulation | denotes | increasing |
| T26474 | 62-69 | Binding | denotes | binding |
| T26475 | 116-124 | Protein | denotes | 3xHA-p65 |
| T26476 | 166-169 | Protein | denotes | Tat |
| T26477 | 179-194 | Protein | denotes | Tat T,N(23,24)A |
| T26478 | 204-217 | Protein | denotes | Tat K(50,51)A |
| T26479 | 227-240 | Protein | denotes | Tat R(49–57)A |
| T26480 | 253-269 | Protein | denotes | Tat C(22,25,27)A |
| T26481 | 278-286 | Protein | denotes | FLAG-Tat |
| T26482 | 487-490 | Protein | denotes | p65 |
| T26483 | 517-524 | Protein | denotes | GST-Tat |
| T26484 | 526-545 | Protein | denotes | GST-Tat T,N(23,24)A |
| T26485 | 547-564 | Protein | denotes | GST-Tat K(50,51)A |
| T26486 | 566-583 | Protein | denotes | GST-Tat R(49–57)A |
| T26487 | 585-605 | Protein | denotes | GST-Tat C(22,25,27)A |
| T26488 | 610-613 | Protein | denotes | GST |
| T26489 | 696-699 | Protein | denotes | GST |
| T26490 | 862-865 | Protein | denotes | p65 |
| T26491 | 921-924 | Protein | denotes | Tat |
| T26492 | 970-978 | Protein | denotes | 3xHA-p65 |
| T26493 | 988-998 | Protein | denotes | 3xHA-p65ΔC |
| T26494 | 1019-1029 | Protein | denotes | 3xHA-p65ΔN |
| T26495 | 1069-1075 | Protein | denotes | HA-p65 |
| T26496 | 1266-1269 | Protein | denotes | p65 |
| R20919 | T26470 | T26471 | themeOf | Tat,associates |
| R20920 | T26471 | T26473 | causeOf | associates,increasing |
| R20921 | T26472 | T26471 | themeOf | p65,associates |
| R20922 | T26472 | T26474 | themeOf | p65,binding |
| R20923 | T26474 | T26473 | themeOf | binding,increasing |
bionlp-st-ge-2016-uniprot
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26505 | 10-13 | P04610 | denotes | Tat |
| T26506 | 10-13 | Q8UMQ1 | denotes | Tat |
| T26507 | 10-13 | P04605 | denotes | Tat |
| T26508 | 10-13 | P04608 | denotes | Tat |
| T26509 | 30-33 | P21579 | denotes | p65 |
| T26510 | 30-33 | Q04206 | denotes | p65 |
| T26511 | 121-124 | P21579 | denotes | p65 |
| T26512 | 121-124 | Q04206 | denotes | p65 |
| T26513 | 166-169 | P04605 | denotes | Tat |
| T26514 | 166-169 | P04608 | denotes | Tat |
| T26515 | 166-169 | P04610 | denotes | Tat |
| T26516 | 166-169 | Q8UMQ1 | denotes | Tat |
| T26517 | 179-182 | P04610 | denotes | Tat |
| T26518 | 179-182 | P04608 | denotes | Tat |
| T26519 | 179-182 | P04605 | denotes | Tat |
| T26520 | 179-182 | Q8UMQ1 | denotes | Tat |
| T26521 | 204-207 | P04608 | denotes | Tat |
| T26522 | 204-207 | P04605 | denotes | Tat |
| T26523 | 204-207 | P04610 | denotes | Tat |
| T26524 | 204-207 | Q8UMQ1 | denotes | Tat |
| T26525 | 227-230 | Q8UMQ1 | denotes | Tat |
| T26526 | 227-230 | P04610 | denotes | Tat |
| T26527 | 227-230 | P04608 | denotes | Tat |
| T26528 | 227-230 | P04605 | denotes | Tat |
| T26529 | 253-256 | Q8UMQ1 | denotes | Tat |
| T26530 | 253-256 | P04610 | denotes | Tat |
| T26531 | 253-256 | P04608 | denotes | Tat |
| T26532 | 253-256 | P04605 | denotes | Tat |
| T26533 | 283-286 | Q8UMQ1 | denotes | Tat |
| T26534 | 283-286 | P04610 | denotes | Tat |
| T26535 | 283-286 | P04608 | denotes | Tat |
| T26536 | 283-286 | P04605 | denotes | Tat |
| T26537 | 487-490 | P21579 | denotes | p65 |
| T26538 | 487-490 | Q04206 | denotes | p65 |
| T26539 | 521-524 | P04608 | denotes | Tat |
| T26540 | 521-524 | P04605 | denotes | Tat |
| T26541 | 521-524 | P04610 | denotes | Tat |
| T26542 | 521-524 | Q8UMQ1 | denotes | Tat |
| T26543 | 530-533 | P04605 | denotes | Tat |
| T26544 | 530-533 | P04610 | denotes | Tat |
| T26545 | 530-533 | P04608 | denotes | Tat |
| T26546 | 530-533 | Q8UMQ1 | denotes | Tat |
| T26547 | 551-554 | P04610 | denotes | Tat |
| T26548 | 551-554 | P04608 | denotes | Tat |
| T26549 | 551-554 | P04605 | denotes | Tat |
| T26550 | 551-554 | Q8UMQ1 | denotes | Tat |
| T26551 | 570-573 | Q8UMQ1 | denotes | Tat |
| T26552 | 570-573 | P04608 | denotes | Tat |
| T26553 | 570-573 | P04610 | denotes | Tat |
| T26554 | 570-573 | P04605 | denotes | Tat |
| T26555 | 589-592 | P04610 | denotes | Tat |
| T26556 | 589-592 | Q8UMQ1 | denotes | Tat |
| T26557 | 589-592 | P04608 | denotes | Tat |
| T26558 | 589-592 | P04605 | denotes | Tat |
| T26559 | 781-784 | P21579 | denotes | p65 |
| T26560 | 781-784 | Q04206 | denotes | p65 |
| T26561 | 862-865 | Q04206 | denotes | p65 |
| T26562 | 862-865 | P21579 | denotes | p65 |
| T26563 | 921-924 | P04608 | denotes | Tat |
| T26564 | 921-924 | P04610 | denotes | Tat |
| T26565 | 921-924 | Q8UMQ1 | denotes | Tat |
| T26566 | 921-924 | P04605 | denotes | Tat |
| T26567 | 975-978 | P21579 | denotes | p65 |
| T26568 | 975-978 | Q04206 | denotes | p65 |
| T26569 | 1072-1075 | Q04206 | denotes | p65 |
| T26570 | 1072-1075 | P21579 | denotes | p65 |
| T26571 | 1266-1269 | P21579 | denotes | p65 |
| T26572 | 1266-1269 | Q04206 | denotes | p65 |
| T26573 | 1446-1449 | Q8UMQ1 | denotes | Tat |
| T26574 | 1446-1449 | P04610 | denotes | Tat |
| T26575 | 1446-1449 | P04608 | denotes | Tat |
| T26576 | 1446-1449 | P04605 | denotes | Tat |
| T26577 | 1458-1461 | Q8UMQ1 | denotes | Tat |
| T26578 | 1458-1461 | P04610 | denotes | Tat |
| T26579 | 1458-1461 | P04608 | denotes | Tat |
| T26580 | 1458-1461 | P04605 | denotes | Tat |