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PMC:3333881 / 26459-30143 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T13335 0-3 NN denotes Tat
T13336 4-13 VB denotes increases
T13337 14-17 DT denotes the
T13338 18-21 NN denotes p65
T13339 22-30 NN denotes affinity
T13340 31-38 NN denotes binding
T13341 39-41 TO denotes to
T13342 42-45 NN denotes DNA
T13343 46-53 IN denotes through
T13344 54-65 NN denotes association
T13345 66-70 IN denotes with
T13346 71-74 NN denotes p65
T13347 75-77 PRP denotes We
T13348 78-84 VB denotes tested
T13349 85-92 IN denotes whether
T13350 93-96 NN denotes Tat
T13351 97-107 RB denotes physically
T13352 108-118 VB denotes interacted
T13353 119-123 IN denotes with
T13354 124-127 NN denotes p65
T13355 129-133 NN denotes HeLa
T13356 134-139 NN denotes cells
T13357 140-144 VB denotes were
T13358 145-156 VB denotes transfected
T13359 157-161 IN denotes with
T13360 162-165 DT denotes the
T13361 166-176 NN denotes expression
T13362 177-184 NN denotes vectors
T13363 185-187 IN denotes of
T13364 188-194 NN denotes HA-p65
T13365 195-198 CC denotes and
T13366 199-207 NN denotes FLAG-Tat
T13367 207-208 -COMMA- denotes ,
T13368 209-212 CC denotes and
T13369 213-217 NN denotes 24 h
T13370 218-223 JJ denotes later
T13371 224-227 NN denotes Tat
T13372 228-231 VB denotes was
T13373 232-250 VB denotes immunoprecipitated
T13374 251-255 IN denotes from
T13375 256-260 NN denotes cell
T13376 261-269 NN denotes extracts
T13377 271-274 DT denotes The
T13378 275-278 NN denotes p65
T13379 279-286 NN denotes protein
T13380 287-290 VB denotes was
T13381 291-311 VB denotes coimmunoprecipitated
T13382 312-316 IN denotes with
T13383 317-326 JJ denotes wild-type
T13384 327-330 NN denotes Tat
T13385 331-333 CC denotes or
T13386 334-337 DT denotes the
T13387 338-345 NN denotes mutants
T13388 346-349 NN denotes Tat
T13389 350-351 NN denotes T
T13390 351-352 -COMMA- denotes ,
T13391 352-353 NN denotes N
T13392 353-354 -LRB- denotes (
T13393 354-359 CD denotes 23,24
T13394 359-360 -RRB- denotes )
T13395 360-361 NN denotes A
T13396 361-362 -COMMA- denotes ,
T13397 363-366 NN denotes Tat
T13398 367-368 NN denotes K
T13399 368-369 -LRB- denotes (
T13400 369-374 CD denotes 50,51
T13401 374-375 -RRB- denotes )
T13402 375-376 NN denotes A
T13403 377-380 CC denotes and
T13404 381-384 NN denotes Tat
T13405 385-386 NN denotes R
T13406 386-387 -LRB- denotes (
T13407 387-392 CD denotes 49–57
T13408 392-393 -RRB- denotes )
T13409 393-394 NN denotes A
T13410 395-397 IN denotes at
T13411 398-405 JJ denotes similar
T13412 406-412 NN denotes levels
T13413 412-413 -COMMA- denotes ,
T13414 414-419 IN denotes while
T13415 420-421 DT denotes a
T13416 422-433 JJ denotes significant
T13417 434-442 NN denotes decrease
T13418 443-445 IN denotes in
T13419 446-467 NN denotes coimmunoprecipitation
T13420 468-471 VB denotes was
T13421 472-480 VB denotes observed
T13422 481-484 IN denotes for
T13423 485-488 NN denotes Tat
T13424 489-490 NN denotes C
T13425 490-491 -LRB- denotes (
T13426 491-499 CD denotes 22,25,27
T13427 499-500 -RRB- denotes )
T13428 500-501 NN denotes A
T13429 502-503 -LRB- denotes (
T13430 503-509 NN denotes Figure
T13431 510-512 NN denotes 4A
T13432 512-513 -RRB- denotes )
T13433 515-527 RB denotes Consistently
T13434 527-528 -COMMA- denotes ,
T13435 529-537 NNP denotes GST-pull
T13436 538-542 IN denotes down
T13437 543-545 IN denotes of
T13438 546-548 FW denotes in
T13439 549-554 FW denotes vitro
T13440 555-565 VB denotes translated
T13441 566-574 NN denotes proteins
T13442 575-581 VB denotes showed
T13443 582-585 DT denotes the
T13444 586-592 JJ denotes direct
T13445 593-600 NN denotes binding
T13446 601-603 IN denotes of
T13447 604-607 NN denotes p65
T13448 608-610 TO denotes to
T13449 611-620 JJ denotes wild-type
T13450 621-624 NN denotes Tat
T13451 624-625 -COMMA- denotes ,
T13452 626-628 CC denotes or
T13453 629-632 DT denotes the
T13454 633-640 NN denotes mutants
T13455 641-644 NN denotes Tat
T13456 645-646 NN denotes T
T13457 646-647 -COMMA- denotes ,
T13458 647-648 NN denotes N
T13459 648-649 -LRB- denotes (
T13460 649-654 CD denotes 23,24
T13461 654-655 -RRB- denotes )
T13462 655-656 NN denotes A
T13463 656-657 -COMMA- denotes ,
T13464 658-661 NN denotes Tat
T13465 662-663 NN denotes K
T13466 663-664 -LRB- denotes (
T13467 664-669 CD denotes 50,51
T13468 669-670 -RRB- denotes )
T13469 670-671 NN denotes A
T13470 672-675 CC denotes and
T13471 676-679 NN denotes Tat
T13472 680-681 NN denotes R
T13473 681-682 -LRB- denotes (
T13474 682-687 CD denotes 49–57
T13475 687-688 -RRB- denotes )
T13476 688-689 NN denotes A
T13477 689-690 -COMMA- denotes ,
T13478 691-694 CC denotes and
T13479 695-699 NN denotes lack
T13480 700-702 IN denotes of
T13481 703-710 VB denotes binding
T13482 711-713 TO denotes to
T13483 714-717 NN denotes Tat
T13484 718-719 NN denotes C
T13485 719-720 -LRB- denotes (
T13486 720-728 CD denotes 22,25,27
T13487 728-729 -RRB- denotes )
T13488 729-730 NN denotes A
T13489 731-732 -LRB- denotes (
T13490 732-738 NN denotes Figure
T13491 739-741 NN denotes 4B
T13492 741-742 -RRB- denotes )
T13493 744-749 DT denotes These
T13494 750-757 NN denotes results
T13495 758-767 VB denotes indicated
T13496 768-772 IN denotes that
T13497 773-776 NN denotes Tat
T13498 777-787 VB denotes associated
T13499 788-792 IN denotes with
T13500 793-796 NN denotes p65
T13501 797-804 IN denotes through
T13502 805-808 DT denotes the
T13503 809-822 JJ denotes cysteine-rich
T13504 823-829 NN denotes domain
T13505 830-833 CC denotes and
T13506 834-843 NN denotes ruled-out
T13507 844-847 DT denotes the
T13508 848-859 NN denotes requirement
T13509 860-862 IN denotes of
T13510 863-871 VB denotes bridging
T13511 872-880 NN denotes proteins
T13512 881-883 TO denotes to
T13513 884-891 VB denotes mediate
T13514 892-896 JJ denotes such
T13515 897-908 NN denotes interaction
T13516 2634-2636 TO denotes To
T13517 2637-2645 VB denotes identify
T13518 2646-2649 DT denotes the
T13519 2650-2653 NN denotes p65
T13520 2654-2660 NN denotes domain
T13521 2661-2669 VB denotes required
T13522 2670-2673 IN denotes for
T13523 2674-2681 VB denotes binding
T13524 2682-2684 TO denotes to
T13525 2685-2688 NN denotes Tat
T13526 2688-2689 -COMMA- denotes ,
T13527 2690-2694 NN denotes HeLa
T13528 2695-2700 NN denotes cells
T13529 2701-2705 VB denotes were
T13530 2706-2717 VB denotes transfected
T13531 2718-2722 IN denotes with
T13532 2723-2731 NN denotes FLAG-Tat
T13533 2732-2740 RB denotes together
T13534 2741-2745 IN denotes with
T13535 2746-2752 NN denotes HA-p65
T13536 2753-2760 NN denotes mutants
T13537 2760-2761 -COMMA- denotes ,
T13538 2762-2767 WDT denotes which
T13539 2768-2772 VB denotes were
T13540 2773-2780 VB denotes deleted
T13541 2781-2783 IN denotes of
T13542 2784-2787 DT denotes the
T13543 2788-2803 NN denotes transactivation
T13544 2804-2810 NN denotes domain
T13545 2811-2812 -LRB- denotes [
T13546 2812-2817 NN denotes p65ΔC
T13547 2818-2819 -LRB- denotes (
T13548 2819-2824 CD denotes 1–318
T13549 2824-2825 -RRB- denotes )
T13550 2825-2826 -RRB- denotes ]
T13551 2826-2827 -COMMA- denotes ,
T13552 2828-2830 CC denotes or
T13553 2831-2834 DT denotes the
T13554 2835-2838 NN denotes RHD
T13555 2839-2840 -LRB- denotes [
T13556 2840-2845 NN denotes p65ΔN
T13557 2846-2847 -LRB- denotes (
T13558 2847-2854 CD denotes 122–551
T13559 2854-2855 -RRB- denotes )
T13560 2855-2856 -RRB- denotes ]
T13561 2857-2858 -LRB- denotes (
T13562 2858-2864 NN denotes Figure
T13563 2865-2867 NN denotes 4C
T13564 2867-2868 -RRB- denotes )
T13565 2870-2873 NN denotes Tat
T13566 2874-2894 VB denotes coimmunoprecipitated
T13567 2895-2899 IN denotes with
T13568 2900-2905 NN denotes p65ΔC
T13569 2906-2907 -LRB- denotes (
T13570 2907-2912 CD denotes 1–318
T13571 2912-2913 -RRB- denotes )
T13572 2913-2914 -COMMA- denotes ,
T13573 2915-2918 CC denotes and
T13574 2919-2922 RB denotes not
T13575 2923-2927 IN denotes with
T13576 2928-2933 NN denotes p65ΔN
T13577 2934-2935 -LRB- denotes (
T13578 2935-2942 CD denotes 122–551
T13579 2942-2943 -RRB- denotes )
T13580 2944-2945 -LRB- denotes (
T13581 2945-2951 NN denotes Figure
T13582 2952-2954 NN denotes 4D
T13583 2954-2955 -RRB- denotes )
T13584 2955-2956 -COMMA- denotes ,
T13585 2957-2967 VB denotes indicating
T13586 2968-2972 IN denotes that
T13587 2973-2976 DT denotes the
T13588 2977-2980 NN denotes RHD
T13589 2981-2983 IN denotes of
T13590 2984-2987 NN denotes p65
T13591 2988-2991 VB denotes was
T13592 2992-3000 VB denotes involved
T13593 3001-3003 IN denotes in
T13594 3004-3007 DT denotes the
T13595 3008-3016 JJ denotes physical
T13596 3017-3028 NN denotes interaction
T13597 3029-3033 IN denotes with
T13598 3034-3037 NN denotes Tat
T13599 3039-3041 TO denotes To
T13600 3042-3049 VB denotes address
T13601 3050-3053 DT denotes the
T13602 3054-3060 NN denotes effect
T13603 3061-3063 IN denotes of
T13604 3064-3067 NN denotes Tat
T13605 3068-3070 IN denotes on
T13606 3071-3074 DT denotes the
T13607 3075-3078 NN denotes p65
T13608 3079-3090 JJ denotes DNA-binding
T13609 3091-3099 NN denotes affinity
T13610 3099-3100 -COMMA- denotes ,
T13611 3101-3109 VB denotes purified
T13612 3110-3121 JJ denotes recombinant
T13613 3122-3125 NN denotes p65
T13614 3126-3133 NN denotes protein
T13615 3134-3137 VB denotes was
T13616 3138-3147 VB denotes incubated
T13617 3148-3152 IN denotes with
T13618 3153-3170 NN denotes 32P-labeled-NF-κB
T13619 3171-3186 JJ denotes double-stranded
T13620 3187-3202 NN denotes oligonucleotide
T13621 3203-3205 IN denotes in
T13622 3206-3214 NN denotes presence
T13623 3215-3217 CC denotes or
T13624 3218-3225 NN denotes absence
T13625 3226-3228 IN denotes of
T13626 3229-3238 JJ denotes wild-type
T13627 3239-3242 NN denotes Tat
T13628 3243-3245 CC denotes or
T13629 3246-3249 NN denotes Tat
T13630 3250-3251 NN denotes C
T13631 3251-3252 -LRB- denotes (
T13632 3252-3260 CD denotes 22,25,27
T13633 3260-3261 -RRB- denotes )
T13634 3261-3262 NN denotes A
T13635 3262-3263 -COMMA- denotes ,
T13636 3264-3267 CC denotes and
T13637 3268-3271 NN denotes p65
T13638 3272-3283 NN denotes DNA-binding
T13639 3284-3287 VB denotes was
T13640 3288-3296 VB denotes analysed
T13641 3297-3299 IN denotes by
T13642 3300-3304 NN denotes EMSA
T13643 3306-3313 IN denotes Whereas
T13644 3314-3323 JJ denotes wild-type
T13645 3324-3327 NN denotes Tat
T13646 3328-3341 RB denotes significantly
T13647 3342-3351 VB denotes increased
T13648 3352-3355 DT denotes the
T13649 3356-3363 NN denotes binding
T13650 3364-3366 IN denotes of
T13651 3367-3370 NN denotes p65
T13652 3371-3373 TO denotes to
T13653 3374-3377 NN denotes DNA
T13654 3377-3378 -COMMA- denotes ,
T13655 3379-3382 DT denotes the
T13656 3383-3389 JJ denotes mutant
T13657 3390-3393 NN denotes Tat
T13658 3394-3395 NN denotes C
T13659 3395-3396 -LRB- denotes (
T13660 3396-3404 CD denotes 22,25,27
T13661 3404-3405 -RRB- denotes )
T13662 3405-3406 NN denotes A
T13663 3406-3407 -COMMA- denotes ,
T13664 3408-3415 VB denotes lacking
T13665 3416-3419 DT denotes the
T13666 3420-3427 VB denotes binding
T13667 3428-3432 NN denotes site
T13668 3433-3436 IN denotes for
T13669 3437-3440 NN denotes p65
T13670 3440-3441 -COMMA- denotes ,
T13671 3442-3445 VB denotes was
T13672 3446-3457 JJ denotes ineffective
T13673 3458-3459 -LRB- denotes (
T13674 3459-3465 NN denotes Figure
T13675 3466-3468 NN denotes 4E
T13676 3468-3469 -RRB- denotes )
T13677 3471-3474 DT denotes The
T13678 3475-3478 NN denotes p65
T13679 3479-3486 NN denotes binding
T13680 3487-3489 TO denotes to
T13681 3490-3493 NN denotes DNA
T13682 3494-3497 VB denotes was
T13683 3498-3507 VB denotes evaluated
T13684 3508-3510 IN denotes by
T13685 3511-3522 NN denotes competition
T13686 3523-3527 IN denotes with
T13687 3528-3538 VB denotes increasing
T13688 3539-3546 NN denotes amounts
T13689 3547-3549 IN denotes of
T13690 3550-3554 JJ denotes cold
T13691 3555-3565 NN denotes competitor
T13692 3566-3571 NN denotes NF-κB
T13693 3572-3587 NN denotes oligonucleotide
T13694 3587-3588 -COMMA- denotes ,
T13695 3589-3594 WDT denotes which
T13696 3595-3601 VB denotes showed
T13697 3602-3606 IN denotes that
T13698 3607-3610 NN denotes Tat
T13699 3611-3624 RB denotes significantly
T13700 3625-3633 VB denotes enhanced
T13701 3634-3637 DT denotes the
T13702 3638-3641 NN denotes p65
T13703 3642-3650 NN denotes affinity
T13704 3651-3658 NN denotes binding
T13705 3659-3661 TO denotes to
T13706 3662-3665 NN denotes DNA
T13707 3666-3667 -LRB- denotes (
T13708 3667-3673 NN denotes Figure
T13709 3674-3676 NN denotes 4E
T13710 3677-3680 CC denotes and
T13711 3681-3682 NN denotes F
T13712 3682-3683 -RRB- denotes )
R10763 T13335 T13336 arg1Of Tat,increases
R10764 T13336 T13343 arg1Of increases,through
R10765 T13340 T13336 arg2Of binding,increases
R10766 T13340 T13337 arg1Of binding,the
R10767 T13340 T13338 arg1Of binding,p65
R10768 T13340 T13339 arg1Of binding,affinity
R10769 T13340 T13341 arg1Of binding,to
R10770 T13342 T13341 arg2Of DNA,to
R10772 T13344 T13343 arg2Of association,through
R10773 T13346 T13345 arg2Of p65,with
R10774 T13347 T13348 arg1Of We,tested
R10775 T13350 T13352 arg1Of Tat,interacted
R10776 T13352 T13348 arg2Of interacted,tested
R10777 T13352 T13349 arg1Of interacted,whether
R10778 T13352 T13351 arg1Of interacted,physically
R10779 T13352 T13353 arg1Of interacted,with
R10780 T13354 T13353 arg2Of p65,with
R10781 T13356 T13355 arg1Of cells,HeLa
R10782 T13356 T13357 arg1Of cells,were
R10783 T13356 T13358 arg2Of cells,transfected
R10784 T13358 T13357 arg2Of transfected,were
R10785 T13358 T13359 arg1Of transfected,with
R10786 T13358 T13368 arg1Of transfected,and
R10787 T13362 T13359 arg2Of vectors,with
R10788 T13362 T13360 arg1Of vectors,the
R10789 T13362 T13361 arg1Of vectors,expression
R10790 T13362 T13363 arg1Of vectors,of
R10791 T13364 T13365 arg1Of HA-p65,and
R10792 T13365 T13363 arg2Of and,of
R10793 T13366 T13365 arg2Of FLAG-Tat,and
R10794 T13368 T13367 arg1Of and,","
R10795 T13371 T13369 arg1Of Tat,24 h
R10796 T13371 T13370 arg1Of Tat,later
R10797 T13371 T13372 arg1Of Tat,was
R10798 T13371 T13373 arg2Of Tat,immunoprecipitated
R10799 T13373 T13368 arg2Of immunoprecipitated,and
R10800 T13373 T13372 arg2Of immunoprecipitated,was
R10801 T13373 T13374 arg1Of immunoprecipitated,from
R10802 T13376 T13374 arg2Of extracts,from
R10803 T13376 T13375 arg1Of extracts,cell
R10804 T13379 T13377 arg1Of protein,The
R10805 T13379 T13378 arg1Of protein,p65
R10806 T13379 T13380 arg1Of protein,was
R10807 T13379 T13381 arg2Of protein,coimmunoprecipitated
R10808 T13381 T13380 arg2Of coimmunoprecipitated,was
R10809 T13381 T13382 arg1Of coimmunoprecipitated,with
R10810 T13381 T13410 arg1Of coimmunoprecipitated,at
R10811 T13381 T13413 arg1Of coimmunoprecipitated,","
R10812 T13381 T13414 arg1Of coimmunoprecipitated,while
R10813 T13384 T13383 arg1Of Tat,wild-type
R10814 T13384 T13385 arg1Of Tat,or
R10815 T13385 T13390 arg1Of or,","
R10816 T13387 T13386 arg1Of mutants,the
R10817 T13389 T13385 arg2Of T,or
R10818 T13389 T13387 arg1Of T,mutants
R10819 T13389 T13388 arg1Of T,Tat
R10820 T13390 T13396 arg1Of ",",","
R10821 T13393 T13392 arg2Of "23,24",(
R10822 T13394 T13392 arg3Of ),(
R10823 T13395 T13390 arg2Of A,","
R10824 T13395 T13391 arg1Of A,N
R10825 T13395 T13392 arg1Of A,(
R10826 T13396 T13403 arg1Of ",",and
R10827 T13400 T13399 arg2Of "50,51",(
R10828 T13401 T13399 arg3Of ),(
R10829 T13402 T13396 arg2Of A,","
R10830 T13402 T13397 arg1Of A,Tat
R10831 T13402 T13398 arg1Of A,K
R10832 T13402 T13399 arg1Of A,(
R10833 T13403 T13382 arg2Of and,with
R10834 T13405 T13404 arg1Of R,Tat
R10835 T13405 T13406 arg1Of R,(
R10836 T13407 T13406 arg2Of 49–57,(
R10837 T13408 T13406 arg3Of ),(
R10838 T13409 T13403 arg2Of A,and
R10839 T13409 T13405 arg1Of A,R
R10840 T13412 T13410 arg2Of levels,at
R10841 T13412 T13411 arg1Of levels,similar
R10842 T13417 T13415 arg1Of decrease,a
R10843 T13417 T13416 arg1Of decrease,significant
R10844 T13417 T13418 arg1Of decrease,in
R10845 T13417 T13420 arg1Of decrease,was
R10846 T13417 T13421 arg2Of decrease,observed
R10847 T13419 T13418 arg2Of coimmunoprecipitation,in
R10848 T13421 T13414 arg2Of observed,while
R10849 T13421 T13420 arg2Of observed,was
R10850 T13421 T13422 arg1Of observed,for
R10851 T13426 T13425 arg2Of "22,25,27",(
R10852 T13427 T13425 arg3Of ),(
R10853 T13428 T13422 arg2Of A,for
R10854 T13428 T13423 arg1Of A,Tat
R10855 T13428 T13424 arg1Of A,C
R10856 T13428 T13425 arg1Of A,(
R10857 T13428 T13429 arg1Of A,(
R10858 T13431 T13429 arg2Of 4A,(
R10859 T13431 T13430 arg1Of 4A,Figure
R10860 T13432 T13429 arg3Of ),(
R10861 T13435 T13442 arg1Of GST-pull,showed
R10862 T13436 T13437 arg1Of down,of
R10863 T13441 T13436 arg2Of proteins,down
R10864 T13441 T13438 arg1Of proteins,in
R10865 T13441 T13439 arg1Of proteins,vitro
R10866 T13441 T13440 arg2Of proteins,translated
R10867 T13442 T13433 arg1Of showed,Consistently
R10868 T13442 T13434 arg1Of showed,","
R10869 T13442 T13436 arg1Of showed,down
R10870 T13445 T13444 arg1Of binding,direct
R10871 T13445 T13446 arg1Of binding,of
R10872 T13445 T13448 arg1Of binding,to
R10873 T13445 T13452 arg1Of binding,or
R10874 T13447 T13446 arg2Of p65,of
R10875 T13450 T13448 arg2Of Tat,to
R10876 T13450 T13449 arg1Of Tat,wild-type
R10877 T13452 T13451 arg1Of or,","
R10878 T13452 T13478 arg1Of or,and
R10879 T13454 T13453 arg1Of mutants,the
R10880 T13456 T13455 arg1Of T,Tat
R10881 T13456 T13457 arg1Of T,","
R10882 T13457 T13463 arg1Of ",",","
R10883 T13460 T13459 arg2Of "23,24",(
R10884 T13461 T13459 arg3Of ),(
R10885 T13462 T13457 arg2Of A,","
R10886 T13462 T13458 arg1Of A,N
R10887 T13462 T13459 arg1Of A,(
R10888 T13463 T13470 arg1Of ",",and
R10889 T13467 T13466 arg2Of "50,51",(
R10890 T13468 T13466 arg3Of ),(
R10891 T13469 T13463 arg2Of A,","
R10892 T13469 T13464 arg1Of A,Tat
R10893 T13469 T13465 arg1Of A,K
R10894 T13469 T13466 arg1Of A,(
R10895 T13470 T13452 arg2Of and,or
R10896 T13470 T13454 arg1Of and,mutants
R10897 T13472 T13471 arg1Of R,Tat
R10898 T13472 T13473 arg1Of R,(
R10899 T13474 T13473 arg2Of 49–57,(
R10900 T13475 T13473 arg3Of ),(
R10901 T13476 T13470 arg2Of A,and
R10902 T13476 T13472 arg1Of A,R
R10903 T13478 T13442 arg2Of and,showed
R10904 T13478 T13443 arg1Of and,the
R10905 T13478 T13477 arg1Of and,","
R10906 T13479 T13478 arg2Of lack,and
R10907 T13479 T13480 arg1Of lack,of
R10908 T13481 T13480 arg2Of binding,of
R10909 T13481 T13482 arg1Of binding,to
R10910 T13486 T13485 arg2Of "22,25,27",(
R10911 T13487 T13485 arg3Of ),(
R10912 T13488 T13482 arg2Of A,to
R10913 T13488 T13483 arg1Of A,Tat
R10914 T13488 T13484 arg1Of A,C
R10915 T13488 T13485 arg1Of A,(
R10916 T13488 T13489 arg1Of A,(
R10917 T13491 T13489 arg2Of 4B,(
R10918 T13491 T13490 arg1Of 4B,Figure
R10919 T13492 T13489 arg3Of ),(
R10920 T13494 T13493 arg1Of results,These
R10921 T13494 T13495 arg1Of results,indicated
R10922 T13497 T13498 arg1Of Tat,associated
R10923 T13498 T13495 arg2Of associated,indicated
R10924 T13498 T13496 arg1Of associated,that
R10925 T13498 T13499 arg1Of associated,with
R10926 T13500 T13501 arg1Of p65,through
R10927 T13500 T13505 arg1Of p65,and
R10928 T13504 T13501 arg2Of domain,through
R10929 T13504 T13502 arg1Of domain,the
R10930 T13504 T13503 arg1Of domain,cysteine-rich
R10931 T13505 T13499 arg2Of and,with
R10932 T13506 T13505 arg2Of ruled-out,and
R10933 T13508 T13506 arg2Of requirement,ruled-out
R10934 T13508 T13507 arg1Of requirement,the
R10935 T13508 T13509 arg1Of requirement,of
R10936 T13510 T13509 arg2Of bridging,of
R10937 T13510 T13512 modOf bridging,to
R10938 T13511 T13510 arg2Of proteins,bridging
R10939 T13513 T13512 arg1Of mediate,to
R10940 T13515 T13513 arg2Of interaction,mediate
R10941 T13515 T13514 arg1Of interaction,such
R10942 T13517 T13516 arg1Of identify,To
R10943 T13520 T13517 arg2Of domain,identify
R10944 T13520 T13518 arg1Of domain,the
R10945 T13520 T13519 arg1Of domain,p65
R10946 T13520 T13521 arg2Of domain,required
R10947 T13521 T13522 arg1Of required,for
R10948 T13523 T13522 arg2Of binding,for
R10949 T13523 T13524 arg1Of binding,to
R10950 T13525 T13524 arg2Of Tat,to
R10951 T13528 T13527 arg1Of cells,HeLa
R10952 T13528 T13529 arg1Of cells,were
R10953 T13528 T13530 arg2Of cells,transfected
R10954 T13530 T13516 modOf transfected,To
R10955 T13530 T13526 arg1Of transfected,","
R10956 T13530 T13529 arg2Of transfected,were
R10957 T13530 T13531 arg1Of transfected,with
R10958 T13532 T13531 arg2Of FLAG-Tat,with
R10959 T13532 T13534 arg1Of FLAG-Tat,with
R10960 T13532 T13561 arg1Of FLAG-Tat,(
R10961 T13534 T13533 arg1Of with,together
R10962 T13536 T13535 arg1Of mutants,HA-p65
R10963 T13536 T13537 arg1Of mutants,","
R10964 T13536 T13538 arg1Of mutants,which
R10965 T13536 T13539 arg1Of mutants,were
R10966 T13536 T13540 arg2Of mutants,deleted
R10967 T13536 T13552 arg1Of mutants,or
R10968 T13540 T13539 arg2Of deleted,were
R10969 T13540 T13541 arg1Of deleted,of
R10970 T13544 T13541 arg2Of domain,of
R10971 T13544 T13542 arg1Of domain,the
R10972 T13544 T13543 arg1Of domain,transactivation
R10973 T13544 T13545 arg1Of domain,[
R10974 T13546 T13545 arg2Of p65ΔC,[
R10975 T13546 T13547 arg1Of p65ΔC,(
R10976 T13548 T13547 arg2Of 1–318,(
R10977 T13549 T13547 arg3Of ),(
R10978 T13550 T13545 arg3Of ],[
R10979 T13552 T13534 arg2Of or,with
R10980 T13552 T13551 arg1Of or,","
R10981 T13554 T13552 arg2Of RHD,or
R10982 T13554 T13553 arg1Of RHD,the
R10983 T13554 T13555 arg1Of RHD,[
R10984 T13556 T13555 arg2Of p65ΔN,[
R10985 T13556 T13557 arg1Of p65ΔN,(
R10986 T13558 T13557 arg2Of 122–551,(
R10987 T13559 T13557 arg3Of ),(
R10988 T13560 T13555 arg3Of ],[
R10989 T13563 T13561 arg2Of 4C,(
R10990 T13563 T13562 arg1Of 4C,Figure
R10991 T13564 T13561 arg3Of ),(
R10992 T13565 T13566 arg1Of Tat,coimmunoprecipitated
R10993 T13566 T13567 arg1Of coimmunoprecipitated,with
R10994 T13566 T13575 arg1Of coimmunoprecipitated,with
R10995 T13566 T13584 arg1Of coimmunoprecipitated,","
R10996 T13566 T13585 modOf coimmunoprecipitated,indicating
R10997 T13567 T13573 arg1Of with,and
R10998 T13568 T13567 arg2Of p65ΔC,with
R10999 T13568 T13569 arg1Of p65ΔC,(
R11000 T13570 T13569 arg2Of 1–318,(
R11001 T13571 T13569 arg3Of ),(
R11002 T13573 T13572 arg1Of and,","
R11003 T13575 T13573 arg2Of with,and
R11004 T13575 T13574 arg1Of with,not
R11005 T13576 T13575 arg2Of p65ΔN,with
R11006 T13576 T13577 arg1Of p65ΔN,(
R11007 T13576 T13580 arg1Of p65ΔN,(
R11008 T13578 T13577 arg2Of 122–551,(
R11009 T13579 T13577 arg3Of ),(
R11010 T13582 T13580 arg2Of 4D,(
R11011 T13582 T13581 arg1Of 4D,Figure
R11012 T13583 T13580 arg3Of ),(
R11013 T13588 T13587 arg1Of RHD,the
R11014 T13588 T13589 arg1Of RHD,of
R11015 T13588 T13591 arg1Of RHD,was
R11016 T13588 T13592 arg2Of RHD,involved
R11017 T13590 T13589 arg2Of p65,of
R11018 T13592 T13585 arg2Of involved,indicating
R11019 T13592 T13586 arg1Of involved,that
R11020 T13592 T13591 arg2Of involved,was
R11021 T13592 T13593 arg1Of involved,in
R11022 T13596 T13593 arg2Of interaction,in
R11023 T13596 T13594 arg1Of interaction,the
R11024 T13596 T13595 arg1Of interaction,physical
R11025 T13596 T13597 arg1Of interaction,with
R11026 T13598 T13597 arg2Of Tat,with
R11027 T13600 T13599 arg1Of address,To
R11028 T13602 T13600 arg2Of effect,address
R11029 T13602 T13601 arg1Of effect,the
R11030 T13602 T13603 arg1Of effect,of
R11031 T13602 T13605 arg1Of effect,on
R11032 T13604 T13603 arg2Of Tat,of
R11033 T13609 T13605 arg2Of affinity,on
R11034 T13609 T13606 arg1Of affinity,the
R11035 T13609 T13607 arg1Of affinity,p65
R11036 T13609 T13608 arg1Of affinity,DNA-binding
R11037 T13614 T13600 arg1Of protein,address
R11038 T13614 T13611 arg2Of protein,purified
R11039 T13614 T13612 arg1Of protein,recombinant
R11040 T13614 T13613 arg1Of protein,p65
R11041 T13614 T13615 arg1Of protein,was
R11042 T13614 T13616 arg2Of protein,incubated
R11043 T13616 T13615 arg2Of incubated,was
R11044 T13616 T13617 arg1Of incubated,with
R11045 T13616 T13636 arg1Of incubated,and
R11046 T13620 T13617 arg2Of oligonucleotide,with
R11047 T13620 T13618 arg1Of oligonucleotide,32P-labeled-NF-κB
R11048 T13620 T13619 arg1Of oligonucleotide,double-stranded
R11049 T13620 T13621 arg1Of oligonucleotide,in
R11050 T13622 T13623 arg1Of presence,or
R11051 T13623 T13621 arg2Of or,in
R11052 T13623 T13625 arg1Of or,of
R11053 T13624 T13623 arg2Of absence,or
R11054 T13627 T13626 arg1Of Tat,wild-type
R11055 T13627 T13628 arg1Of Tat,or
R11056 T13628 T13625 arg2Of or,of
R11057 T13632 T13631 arg2Of "22,25,27",(
R11058 T13633 T13631 arg3Of ),(
R11059 T13634 T13628 arg2Of A,or
R11060 T13634 T13629 arg1Of A,Tat
R11061 T13634 T13630 arg1Of A,C
R11062 T13634 T13631 arg1Of A,(
R11063 T13636 T13599 modOf and,To
R11064 T13636 T13610 arg1Of and,","
R11065 T13636 T13635 arg1Of and,","
R11066 T13638 T13637 arg1Of DNA-binding,p65
R11067 T13638 T13639 arg1Of DNA-binding,was
R11068 T13638 T13640 arg2Of DNA-binding,analysed
R11069 T13640 T13636 arg2Of analysed,and
R11070 T13640 T13639 arg2Of analysed,was
R11071 T13642 T13640 arg1Of EMSA,analysed
R11072 T13642 T13641 arg2Of EMSA,by
R11073 T13645 T13644 arg1Of Tat,wild-type
R11074 T13645 T13647 arg1Of Tat,increased
R11075 T13647 T13643 arg2Of increased,Whereas
R11076 T13647 T13646 arg1Of increased,significantly
R11077 T13649 T13647 arg2Of binding,increased
R11078 T13649 T13648 arg1Of binding,the
R11079 T13649 T13650 arg1Of binding,of
R11080 T13649 T13652 arg1Of binding,to
R11081 T13651 T13650 arg2Of p65,of
R11082 T13653 T13652 arg2Of DNA,to
R11083 T13660 T13659 arg2Of "22,25,27",(
R11084 T13661 T13659 arg3Of ),(
R11085 T13662 T13655 arg1Of A,the
R11086 T13662 T13656 arg1Of A,mutant
R11087 T13662 T13657 arg1Of A,Tat
R11088 T13662 T13658 arg1Of A,C
R11089 T13662 T13659 arg1Of A,(
R11090 T13662 T13663 arg1Of A,","
R11091 T13662 T13671 arg1Of A,was
R11092 T13662 T13672 arg1Of A,ineffective
R11093 T13664 T13663 arg2Of lacking,","
R11094 T13667 T13664 arg2Of site,lacking
R11095 T13667 T13665 arg1Of site,the
R11096 T13667 T13666 arg1Of site,binding
R11097 T13667 T13668 arg1Of site,for
R11098 T13669 T13668 arg2Of p65,for
R11099 T13671 T13643 arg1Of was,Whereas
R11100 T13671 T13654 arg1Of was,","
R11101 T13671 T13670 arg1Of was,","
R11102 T13672 T13671 arg2Of ineffective,was
R11103 T13672 T13673 arg1Of ineffective,(
R11104 T13675 T13673 arg2Of 4E,(
R11105 T13675 T13674 arg1Of 4E,Figure
R11106 T13676 T13673 arg3Of ),(
R11107 T13679 T13677 arg1Of binding,The
R11108 T13679 T13678 arg1Of binding,p65
R11109 T13679 T13680 arg1Of binding,to
R11110 T13679 T13682 arg1Of binding,was
R11111 T13679 T13683 arg2Of binding,evaluated
R11112 T13681 T13680 arg2Of DNA,to
R11113 T13683 T13682 arg2Of evaluated,was
R11114 T13685 T13683 arg1Of competition,evaluated
R11115 T13685 T13684 arg2Of competition,by
R11116 T13685 T13686 arg1Of competition,with
R11117 T13688 T13686 arg2Of amounts,with
R11118 T13688 T13687 arg1Of amounts,increasing
R11119 T13688 T13689 arg1Of amounts,of
R11120 T13693 T13689 arg2Of oligonucleotide,of
R11121 T13693 T13690 arg1Of oligonucleotide,cold
R11122 T13693 T13691 arg1Of oligonucleotide,competitor
R11123 T13693 T13692 arg1Of oligonucleotide,NF-κB
R11124 T13693 T13694 arg1Of oligonucleotide,","
R11125 T13693 T13695 arg1Of oligonucleotide,which
R11126 T13693 T13696 arg1Of oligonucleotide,showed
R11127 T13698 T13700 arg1Of Tat,enhanced
R11128 T13700 T13696 arg2Of enhanced,showed
R11129 T13700 T13697 arg1Of enhanced,that
R11130 T13700 T13699 arg1Of enhanced,significantly
R11131 T13704 T13700 arg2Of binding,enhanced
R11132 T13704 T13701 arg1Of binding,the
R11133 T13704 T13702 arg1Of binding,p65
R11134 T13704 T13703 arg1Of binding,affinity
R11135 T13704 T13705 arg1Of binding,to
R11136 T13704 T13707 arg1Of binding,(
R11137 T13706 T13705 arg2Of DNA,to
R11138 T13709 T13708 arg1Of 4E,Figure
R11139 T13709 T13710 arg1Of 4E,and
R11140 T13710 T13707 arg2Of and,(
R11141 T13711 T13710 arg2Of F,and
R11142 T13712 T13707 arg3Of ),(
R10771 T13344 T13345 arg1Of association,with

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T13716 381-394 Antecedent denotes Tat R(49–57)A
T13717 629-640 Anaphor denotes the mutants
T13718 641-656 Antecedent denotes Tat T,N(23,24)A
T13719 658-671 Antecedent denotes Tat K(50,51)A
T13720 676-689 Antecedent denotes Tat R(49–57)A
T13721 892-908 Anaphor denotes such interaction
T13722 777-787 Antecedent denotes associated
T13713 334-345 Anaphor denotes the mutants
T13714 346-361 Antecedent denotes Tat T,N(23,24)A
T13715 363-376 Antecedent denotes Tat K(50,51)A
T26503 955-958 Anaphor denotes its
T26504 940-943 Antecedent denotes p65
R11143 T13713 T13714 boundBy the mutants,"Tat T,N(23,24)A"
R11144 T13713 T13715 boundBy the mutants,"Tat K(50,51)A"
R11145 T13713 T13716 boundBy the mutants,Tat R(49–57)A
R11146 T13717 T13718 boundBy the mutants,"Tat T,N(23,24)A"
R11147 T13717 T13719 boundBy the mutants,"Tat K(50,51)A"
R11148 T13717 T13720 boundBy the mutants,Tat R(49–57)A
R11149 T13721 T13722 boundBy such interaction,associated
R20924 T26503 T26504 boundBy its,p65

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T13857 0-3 NNP denotes Tat
T13858 4-13 VBZ denotes increases
T13859 14-17 DT denotes the
T13860 18-21 CD denotes p65
T13861 22-30 NN denotes affinity
T13862 31-38 JJ denotes binding
T13863 39-41 TO denotes to
T13864 42-45 NNP denotes DNA
T13865 46-53 IN denotes through
T13866 54-65 NN denotes association
T13867 66-70 IN denotes with
T13868 71-74 CD denotes p65
T13869 75-77 PRP denotes We
T13870 78-84 VBD denotes tested
T13871 85-92 IN denotes whether
T13872 93-96 NNP denotes Tat
T13873 97-107 RB denotes physically
T13874 108-118 VBD denotes interacted
T13875 119-123 IN denotes with
T13876 124-127 NNS denotes p65
T13877 127-128 . denotes .
T13878 129-133 NNP denotes HeLa
T13879 134-139 NNS denotes cells
T13880 140-144 VBD denotes were
T13881 145-156 VBN denotes transfected
T13882 157-161 IN denotes with
T13883 162-165 DT denotes the
T13884 166-176 NN denotes expression
T13885 177-184 NNS denotes vectors
T13886 185-187 IN denotes of
T13887 188-194 NNP denotes HA-p65
T13888 195-198 CC denotes and
T13889 199-207 NNP denotes FLAG-Tat
T13890 207-208 , denotes ,
T13891 209-212 CC denotes and
T13892 213-215 CD denotes 24
T13893 216-217 NN denotes h
T13894 218-223 RB denotes later
T13895 224-227 NNP denotes Tat
T13896 228-231 VBD denotes was
T13897 232-250 VBN denotes immunoprecipitated
T13898 251-255 IN denotes from
T13899 256-260 NN denotes cell
T13900 261-269 NNS denotes extracts
T13901 269-270 . denotes .
T13902 271-274 DT denotes The
T13903 275-278 CD denotes p65
T13904 279-286 NN denotes protein
T13905 287-290 VBD denotes was
T13906 291-311 VBN denotes coimmunoprecipitated
T13907 312-316 IN denotes with
T13908 317-326 JJ denotes wild-type
T13909 327-330 NNP denotes Tat
T13910 331-333 CC denotes or
T13911 334-337 DT denotes the
T13912 338-345 NNS denotes mutants
T13913 346-349 NNP denotes Tat
T13914 350-351 NNP denotes T
T13915 351-352 , denotes ,
T13916 352-353 NNP denotes N
T13917 353-354 -LRB- denotes (
T13918 354-359 CD denotes 23,24
T13919 359-360 -RRB- denotes )
T13920 360-361 DT denotes A
T13921 361-362 , denotes ,
T13922 363-366 NNP denotes Tat
T13923 367-368 NNP denotes K
T13924 368-369 -LRB- denotes (
T13925 369-374 CD denotes 50,51
T13926 374-375 -RRB- denotes )
T13927 375-376 DT denotes A
T13928 377-380 CC denotes and
T13929 381-384 NNP denotes Tat
T13930 385-386 NNP denotes R
T13942 386-387 -LRB- denotes (
T13943 387-389 CD denotes 49
T13944 390-392 CD denotes 57
T13945 392-393 -RRB- denotes )
T13946 393-394 DT denotes A
T13947 395-397 IN denotes at
T13948 398-405 JJ denotes similar
T13949 406-412 NNS denotes levels
T13950 412-413 , denotes ,
T13951 414-419 IN denotes while
T13952 420-421 DT denotes a
T13953 422-433 JJ denotes significant
T13954 434-442 NN denotes decrease
T13955 443-445 IN denotes in
T13956 446-467 NN denotes coimmunoprecipitation
T13957 468-471 VBD denotes was
T13958 472-480 VBN denotes observed
T13959 481-484 IN denotes for
T13960 485-488 NNP denotes Tat
T13961 489-490 NNP denotes C
T13962 490-491 -LRB- denotes (
T13963 491-499 CD denotes 22,25,27
T13964 499-500 -RRB- denotes )
T13965 500-501 DT denotes A
T13966 502-503 -LRB- denotes (
T13967 503-509 NN denotes Figure
T13968 510-512 NN denotes 4A
T13969 512-513 -RRB- denotes )
T13970 513-514 . denotes .
T13971 515-527 RB denotes Consistently
T13972 527-528 , denotes ,
T13973 529-537 NNP denotes GST-pull
T13974 538-542 IN denotes down
T13975 543-545 IN denotes of
T13976 546-548 IN denotes in
T13977 549-554 NN denotes vitro
T13978 555-565 VBN denotes translated
T13979 566-574 NNS denotes proteins
T13980 575-581 VBD denotes showed
T13981 582-585 DT denotes the
T13982 586-592 JJ denotes direct
T13983 593-600 JJ denotes binding
T13984 601-603 IN denotes of
T13985 604-607 CD denotes p65
T13986 608-610 TO denotes to
T13987 611-620 JJ denotes wild-type
T13988 621-624 NNP denotes Tat
T13989 624-625 , denotes ,
T13990 626-628 CC denotes or
T13991 629-632 DT denotes the
T13992 633-640 NNS denotes mutants
T13993 641-644 NNP denotes Tat
T13994 645-646 NNP denotes T
T13995 646-647 , denotes ,
T13996 647-648 NNP denotes N
T13997 648-649 -LRB- denotes (
T13998 649-654 CD denotes 23,24
T13999 654-655 -RRB- denotes )
T14000 655-656 DT denotes A
T14001 656-657 , denotes ,
T14002 658-661 NNP denotes Tat
T14003 662-663 NNP denotes K
T14004 663-664 -LRB- denotes (
T14005 664-669 CD denotes 50,51
T14006 669-670 -RRB- denotes )
T14007 670-671 DT denotes A
T14008 672-675 CC denotes and
T14009 676-679 NNP denotes Tat
T14010 680-681 NNP denotes R
T14011 681-682 -LRB- denotes (
T14012 682-684 CD denotes 49
T14013 685-687 CD denotes 57
T14014 687-688 -RRB- denotes )
T14015 688-689 DT denotes A
T14016 689-690 , denotes ,
T14017 691-694 CC denotes and
T14018 695-699 NN denotes lack
T14019 700-702 IN denotes of
T14020 703-710 JJ denotes binding
T14021 711-713 TO denotes to
T14022 714-717 NNP denotes Tat
T14023 718-719 NNP denotes C
T14024 719-720 -LRB- denotes (
T14025 720-728 CD denotes 22,25,27
T14026 728-729 -RRB- denotes )
T14027 729-730 DT denotes A
T14028 731-732 -LRB- denotes (
T14029 732-738 NN denotes Figure
T14030 739-741 NN denotes 4B
T14031 741-742 -RRB- denotes )
T14032 742-743 . denotes .
T14033 744-749 DT denotes These
T14034 750-757 NNS denotes results
T14035 758-767 VBD denotes indicated
T14036 768-772 IN denotes that
T14037 773-776 NNP denotes Tat
T14038 777-787 VBN denotes associated
T14039 788-792 IN denotes with
T14040 793-796 CD denotes p65
T14041 797-804 IN denotes through
T14042 805-808 DT denotes the
T14043 809-822 JJ denotes cysteine-rich
T14044 823-829 NN denotes domain
T14045 830-833 CC denotes and
T14046 834-843 NN denotes ruled-out
T14047 844-847 DT denotes the
T14048 848-859 NN denotes requirement
T14049 860-862 IN denotes of
T14050 863-871 VBG denotes bridging
T14051 872-880 NNS denotes proteins
T14052 881-883 TO denotes to
T14053 884-891 VB denotes mediate
T14054 892-896 JJ denotes such
T14055 897-908 NN denotes interaction
T14056 908-909 . denotes .
T14057 2634-2636 TO denotes To
T14058 2637-2645 VB denotes identify
T14059 2646-2649 DT denotes the
T14060 2650-2653 NNS denotes p65
T14061 2654-2660 NN denotes domain
T14062 2661-2669 VBN denotes required
T14063 2670-2673 IN denotes for
T14064 2674-2681 JJ denotes binding
T14065 2682-2684 TO denotes to
T14066 2685-2688 NNP denotes Tat
T14067 2688-2689 , denotes ,
T14068 2690-2694 NNP denotes HeLa
T14069 2695-2700 NNS denotes cells
T14070 2701-2705 VBD denotes were
T14071 2706-2717 VBN denotes transfected
T14072 2718-2722 IN denotes with
T14073 2723-2731 JJ denotes FLAG-Tat
T14074 2732-2740 RB denotes together
T14075 2741-2745 IN denotes with
T14076 2746-2752 JJ denotes HA-p65
T14077 2753-2760 NNS denotes mutants
T14078 2760-2761 , denotes ,
T14079 2762-2767 WDT denotes which
T14080 2768-2772 VBD denotes were
T14081 2773-2780 VBN denotes deleted
T14082 2781-2783 IN denotes of
T14083 2784-2787 DT denotes the
T14084 2788-2803 NN denotes transactivation
T14085 2804-2810 NN denotes domain
T14086 2811-2812 NNP denotes [
T14087 2812-2817 NNP denotes p65ΔC
T14088 2818-2819 -LRB- denotes (
T14089 2819-2820 CD denotes 1
T14090 2821-2824 CD denotes 318
T14091 2824-2825 -RRB- denotes )
T14092 2825-2826 NNP denotes ]
T14093 2826-2827 , denotes ,
T14094 2828-2830 CC denotes or
T14095 2831-2834 DT denotes the
T14096 2835-2838 NNP denotes RHD
T14097 2839-2840 NNP denotes [
T14098 2840-2845 NNP denotes p65ΔN
T14099 2846-2847 -LRB- denotes (
T14100 2847-2850 CD denotes 122
T14101 2851-2854 CD denotes 551
T14102 2854-2855 -RRB- denotes )
T14103 2855-2856 NNP denotes ]
T14104 2857-2858 -LRB- denotes (
T14105 2858-2864 NNP denotes Figure
T14106 2865-2867 NNP denotes 4C
T14107 2867-2868 -RRB- denotes )
T14108 2868-2869 . denotes .
T14109 2870-2873 NNP denotes Tat
T14110 2874-2894 VBD denotes coimmunoprecipitated
T14111 2895-2899 IN denotes with
T14112 2900-2905 NNP denotes p65ΔC
T14113 2906-2907 -LRB- denotes (
T14114 2907-2908 CD denotes 1
T14115 2909-2912 CD denotes 318
T14116 2912-2913 -RRB- denotes )
T14117 2913-2914 , denotes ,
T14118 2915-2918 CC denotes and
T14119 2919-2922 RB denotes not
T14120 2923-2927 IN denotes with
T14121 2928-2933 NNP denotes p65ΔN
T14122 2934-2935 -LRB- denotes (
T14123 2935-2938 CD denotes 122
T14124 2939-2942 CD denotes 551
T14125 2942-2943 -RRB- denotes )
T14126 2944-2945 -LRB- denotes (
T14127 2945-2951 NN denotes Figure
T14128 2952-2954 CD denotes 4D
T14129 2954-2955 -RRB- denotes )
T14130 2955-2956 , denotes ,
T14131 2957-2967 VBG denotes indicating
T14132 2968-2972 IN denotes that
T14133 2973-2976 DT denotes the
T14134 2977-2980 NNP denotes RHD
T14135 2981-2983 IN denotes of
T14136 2984-2987 CD denotes p65
T14137 2988-2991 VBD denotes was
T14138 2992-3000 VBN denotes involved
T14139 3001-3003 IN denotes in
T14140 3004-3007 DT denotes the
T14141 3008-3016 JJ denotes physical
T14142 3017-3028 NN denotes interaction
T14143 3029-3033 IN denotes with
T14144 3034-3037 NNP denotes Tat
T14145 3037-3038 . denotes .
T14146 3039-3041 TO denotes To
T14147 3042-3049 VB denotes address
T14148 3050-3053 DT denotes the
T14149 3054-3060 NN denotes effect
T14150 3061-3063 IN denotes of
T14151 3064-3067 NNP denotes Tat
T14152 3068-3070 IN denotes on
T14153 3071-3074 DT denotes the
T14154 3075-3078 CD denotes p65
T14155 3079-3090 JJ denotes DNA-binding
T14156 3091-3099 NN denotes affinity
T14157 3099-3100 , denotes ,
T14158 3101-3109 VBN denotes purified
T14159 3110-3121 JJ denotes recombinant
T14160 3122-3125 NNS denotes p65
T14161 3126-3133 NN denotes protein
T14162 3134-3137 VBD denotes was
T14163 3138-3147 VBN denotes incubated
T14164 3148-3152 IN denotes with
T14165 3153-3170 JJ denotes 32P-labeled-NF-κB
T14166 3171-3186 JJ denotes double-stranded
T14167 3187-3202 NN denotes oligonucleotide
T14168 3203-3205 IN denotes in
T14169 3206-3214 NN denotes presence
T14170 3215-3217 CC denotes or
T14171 3218-3225 NN denotes absence
T14172 3226-3228 IN denotes of
T14173 3229-3238 JJ denotes wild-type
T14174 3239-3242 NNP denotes Tat
T14175 3243-3245 CC denotes or
T14176 3246-3249 NNP denotes Tat
T14177 3250-3251 NNP denotes C
T14178 3251-3252 -LRB- denotes (
T14179 3252-3260 CD denotes 22,25,27
T14180 3260-3261 -RRB- denotes )
T14181 3261-3262 DT denotes A
T14182 3262-3263 , denotes ,
T14183 3264-3267 CC denotes and
T14184 3268-3271 CD denotes p65
T14185 3272-3283 JJ denotes DNA-binding
T14186 3284-3287 VBD denotes was
T14187 3288-3296 VBN denotes analysed
T14188 3297-3299 IN denotes by
T14189 3300-3304 NNP denotes EMSA
T14190 3304-3305 . denotes .
T14191 3306-3313 IN denotes Whereas
T14192 3314-3323 JJ denotes wild-type
T14193 3324-3327 NNP denotes Tat
T14194 3328-3341 RB denotes significantly
T14195 3342-3351 VBD denotes increased
T14196 3352-3355 DT denotes the
T14197 3356-3363 JJ denotes binding
T14198 3364-3366 IN denotes of
T14199 3367-3370 CD denotes p65
T14200 3371-3373 TO denotes to
T14201 3374-3377 NNP denotes DNA
T14202 3377-3378 , denotes ,
T14203 3379-3382 DT denotes the
T14204 3383-3389 JJ denotes mutant
T14205 3390-3393 NNP denotes Tat
T14206 3394-3395 NNP denotes C
T14207 3395-3396 -LRB- denotes (
T14208 3396-3404 CD denotes 22,25,27
T14209 3404-3405 -RRB- denotes )
T14210 3405-3406 DT denotes A
T14211 3406-3407 , denotes ,
T14212 3408-3415 VBG denotes lacking
T14213 3416-3419 DT denotes the
T14214 3420-3427 JJ denotes binding
T14215 3428-3432 NN denotes site
T14216 3433-3436 IN denotes for
T14217 3437-3440 NNS denotes p65
T14218 3440-3441 , denotes ,
T14219 3442-3445 VBD denotes was
T14220 3446-3457 JJ denotes ineffective
T14221 3458-3459 -LRB- denotes (
T14222 3459-3465 NN denotes Figure
T14223 3466-3468 CD denotes 4E
T14224 3468-3469 -RRB- denotes )
T14225 3469-3470 . denotes .
T14226 3471-3474 DT denotes The
T14227 3475-3478 CD denotes p65
T14228 3479-3486 JJ denotes binding
T14229 3487-3489 TO denotes to
T14230 3490-3493 NNP denotes DNA
T14231 3494-3497 VBD denotes was
T14232 3498-3507 VBN denotes evaluated
T14233 3508-3510 IN denotes by
T14234 3511-3522 NN denotes competition
T14235 3523-3527 IN denotes with
T14236 3528-3538 VBG denotes increasing
T14237 3539-3546 NNS denotes amounts
T14238 3547-3549 IN denotes of
T14239 3550-3554 JJ denotes cold
T14240 3555-3565 NN denotes competitor
T14241 3566-3571 NN denotes NF-κB
T14242 3572-3587 NN denotes oligonucleotide
T14243 3587-3588 , denotes ,
T14244 3589-3594 WDT denotes which
T14245 3595-3601 VBD denotes showed
T14246 3602-3606 IN denotes that
T14247 3607-3610 NNP denotes Tat
T14248 3611-3624 RB denotes significantly
T14249 3625-3633 VBD denotes enhanced
T14250 3634-3637 DT denotes the
T14251 3638-3641 CD denotes p65
T14252 3642-3650 NN denotes affinity
T14253 3651-3658 JJ denotes binding
T14254 3659-3661 TO denotes to
T14255 3662-3665 NNP denotes DNA
T14256 3666-3667 -LRB- denotes (
T14257 3667-3673 NNP denotes Figure
T14258 3674-3676 NNP denotes 4E
T14259 3677-3680 CC denotes and
T14260 3681-3682 NN denotes F
T14261 3682-3683 -RRB- denotes )
T14262 3683-3684 . denotes .
T26585 920-923 NNP denotes Tat
T26586 924-934 NNS denotes associates
T26587 935-939 IN denotes with
T26588 940-943 CD denotes p65
T26589 944-954 VBG denotes increasing
T26590 955-958 PRP$ denotes its
T26591 959-971 JJ denotes DNA-affinity
T26592 972-979 JJ denotes binding
T26593 979-980 . denotes .
T26594 981-982 -LRB- denotes (
T26595 982-983 NNP denotes A
T26596 983-984 -RRB- denotes )
T26597 985-989 NNP denotes HeLa
T26598 990-995 NNS denotes cells
T26599 996-1000 VBD denotes were
T26600 1001-1012 VBN denotes transfected
T26601 1013-1017 IN denotes with
T26602 1018-1025 NNP denotes pRc/CMV
T26603 1025-1027 CD denotes -3
T26604 1027-1034 NNP denotes xHA-p65
T26605 1035-1036 -LRB- denotes (
T26606 1036-1037 CD denotes 5
T26607 1038-1040 NN denotes µg
T26608 1040-1041 -RRB- denotes )
T26609 1042-1044 IN denotes in
T26610 1045-1053 NN denotes presence
T26611 1054-1056 CC denotes or
T26612 1057-1064 NN denotes absence
T26613 1065-1067 IN denotes of
T26614 1068-1079 NN denotes p3xFLAG-Tat
T26615 1079-1080 , denotes ,
T26616 1081-1092 NN denotes p3xFLAG-Tat
T26617 1093-1094 NNP denotes T
T26618 1094-1095 , denotes ,
T26619 1095-1096 NNP denotes N
T26620 1096-1097 -LRB- denotes (
T26621 1097-1102 CD denotes 23,24
T26622 1102-1103 -RRB- denotes )
T26623 1103-1104 DT denotes A
T26624 1104-1105 , denotes ,
T26625 1106-1117 JJ denotes p3xFLAG-Tat
T26626 1118-1119 NNP denotes K
T26627 1119-1120 -LRB- denotes (
T26628 1120-1125 CD denotes 50,51
T26629 1125-1126 -RRB- denotes )
T26630 1126-1127 DT denotes A
T26631 1127-1128 , denotes ,
T26632 1129-1140 JJ denotes p3xFLAG-Tat
T26633 1141-1142 NN denotes R
T26634 1142-1143 -LRB- denotes (
T26635 1143-1145 CD denotes 49
T26636 1146-1148 CD denotes 57
T26637 1148-1149 -RRB- denotes )
T26638 1149-1150 DT denotes A
T26639 1150-1151 , denotes ,
T26640 1152-1154 CC denotes or
T26641 1155-1166 NN denotes p3xFLAG-Tat
T26642 1167-1168 NNP denotes C
T26643 1168-1169 -LRB- denotes (
T26644 1169-1177 CD denotes 22,25,27
T26645 1177-1178 -RRB- denotes )
T26646 1178-1179 DT denotes A
T26647 1180-1181 -LRB- denotes (
T26648 1181-1182 CD denotes 5
T26649 1183-1185 NN denotes µg
T26650 1185-1186 -RRB- denotes )
T26651 1186-1187 . denotes .
T26652 1188-1196 JJ denotes FLAG-Tat
T26653 1197-1205 NNS denotes proteins
T26654 1206-1210 VBD denotes were
T26655 1211-1229 VBN denotes immunoprecipitated
T26656 1230-1234 IN denotes with
T26657 1235-1244 JJ denotes anti-FLAG
T26658 1245-1253 NN denotes antibody
T26659 1253-1254 : denotes ;
T26660 1255-1270 NNS denotes immunocomplexes
T26661 1271-1275 VBD denotes were
T26662 1276-1285 VBN denotes separated
T26663 1286-1288 IN denotes by
T26664 1289-1291 CD denotes 12
T26665 1291-1292 NN denotes %
T26666 1293-1296 NNP denotes SDS
T26667 1297-1301 NNP denotes PAGE
T26668 1302-1305 CC denotes and
T26669 1306-1314 VBN denotes analysed
T26670 1315-1317 IN denotes by
T26671 1318-1325 JJ denotes western
T26672 1326-1334 VBG denotes blotting
T26673 1335-1339 IN denotes with
T26674 1340-1349 JJ denotes anti-FLAG
T26675 1350-1352 CC denotes or
T26676 1353-1360 JJ denotes anti-HA
T26677 1361-1371 NNS denotes antibodies
T26678 1371-1372 . denotes .
T26679 1373-1374 -LRB- denotes (
T26680 1374-1375 NNP denotes B
T26681 1375-1376 -RRB- denotes )
T26682 1377-1379 IN denotes In
T26683 1380-1385 NN denotes vitro
T26684 1386-1396 VBN denotes translated
T26685 1397-1400 NNS denotes p65
T26686 1401-1402 -LRB- denotes (
T26687 1402-1403 CD denotes 5
T26688 1404-1406 NN denotes µl
T26689 1406-1407 -RRB- denotes )
T26690 1408-1411 VBD denotes was
T26691 1412-1421 VBN denotes incubated
T26692 1422-1426 IN denotes with
T26693 1427-1434 NNP denotes GST-Tat
T26694 1434-1435 , denotes ,
T26695 1436-1443 NNP denotes GST-Tat
T26696 1444-1445 NNP denotes T
T26697 1445-1446 , denotes ,
T26698 1446-1447 NNP denotes N
T26699 1447-1448 -LRB- denotes (
T26700 1448-1453 CD denotes 23,24
T26701 1453-1454 -RRB- denotes )
T26702 1454-1455 DT denotes A
T26703 1455-1456 , denotes ,
T26704 1457-1464 NNP denotes GST-Tat
T26705 1465-1466 NNP denotes K
T26706 1466-1467 -LRB- denotes (
T26707 1467-1472 CD denotes 50,51
T26708 1472-1473 -RRB- denotes )
T26709 1473-1474 DT denotes A
T26710 1474-1475 , denotes ,
T26711 1476-1483 NNP denotes GST-Tat
T26712 1484-1485 NNP denotes R
T26713 1485-1486 -LRB- denotes (
T26714 1486-1488 CD denotes 49
T26715 1489-1491 CD denotes 57
T26716 1491-1492 -RRB- denotes )
T26717 1492-1493 DT denotes A
T26718 1493-1494 , denotes ,
T26719 1495-1502 NNP denotes GST-Tat
T26720 1503-1504 NNP denotes C
T26721 1504-1505 -LRB- denotes (
T26722 1505-1513 CD denotes 22,25,27
T26723 1513-1514 -RRB- denotes )
T26724 1514-1515 DT denotes A
T26725 1515-1516 , denotes ,
T26726 1517-1519 CC denotes or
T26727 1520-1523 NNP denotes GST
T26728 1524-1525 -LRB- denotes (
T26729 1525-1526 CD denotes 5
T26730 1527-1529 NN denotes µg
T26731 1529-1530 -RRB- denotes )
T26732 1531-1541 VBN denotes conjugated
T26733 1542-1546 IN denotes with
T26734 1547-1568 NNP denotes Glutathione-Sepharose
T26735 1568-1569 . denotes .
T26736 1570-1577 NN denotes Protein
T26737 1578-1587 NNS denotes complexes
T26738 1588-1592 VBD denotes were
T26739 1593-1602 VBN denotes recovered
T26740 1603-1605 IN denotes by
T26741 1606-1614 NNP denotes GST-pull
T26742 1615-1619 RB denotes down
T26743 1619-1620 , denotes ,
T26744 1621-1630 VBN denotes separated
T26745 1631-1633 IN denotes by
T26746 1634-1636 CD denotes 12
T26747 1636-1637 NN denotes %
T26748 1638-1641 NNP denotes SDS
T26749 1642-1646 NNP denotes PAGE
T26750 1646-1647 , denotes ,
T26751 1648-1651 CC denotes and
T26752 1652-1660 VBN denotes analysed
T26753 1661-1663 IN denotes by
T26754 1664-1671 JJ denotes western
T26755 1672-1680 VBG denotes blotting
T26756 1681-1685 IN denotes with
T26757 1686-1694 JJ denotes anti-p65
T26758 1695-1697 CC denotes or
T26759 1698-1706 JJ denotes anti-GST
T26760 1707-1717 NNS denotes antibodies
T26761 1717-1718 . denotes .
T26762 1719-1720 -LRB- denotes (
T26763 1720-1721 $ denotes C
T26764 1721-1722 -RRB- denotes )
T26765 1723-1732 JJ denotes Schematic
T26766 1733-1747 NN denotes representation
T26767 1748-1750 IN denotes of
T26768 1751-1760 JJ denotes wild-type
T26769 1761-1764 CC denotes and
T26770 1765-1771 JJ denotes mutant
T26771 1772-1775 NNS denotes p65
T26772 1776-1784 NNS denotes proteins
T26773 1784-1785 . denotes .
T26774 1786-1787 -LRB- denotes (
T26775 1787-1788 NNP denotes D
T26776 1788-1789 -RRB- denotes )
T26777 1790-1794 NNP denotes HeLa
T26778 1795-1800 NNS denotes cells
T26779 1801-1805 VBD denotes were
T26780 1806-1817 VBN denotes transfected
T26781 1818-1822 IN denotes with
T26782 1823-1834 NN denotes p3xFLAG-Tat
T26783 1835-1836 -LRB- denotes (
T26784 1836-1837 CD denotes 5
T26785 1838-1840 NN denotes µg
T26786 1840-1841 -RRB- denotes )
T26787 1842-1844 IN denotes in
T26788 1845-1848 DT denotes the
T26789 1849-1857 NN denotes presence
T26790 1858-1860 CC denotes or
T26791 1861-1868 NN denotes absence
T26792 1869-1871 IN denotes of
T26793 1872-1879 NNP denotes pRc/CMV
T26794 1879-1881 CD denotes -3
T26795 1881-1888 NNP denotes xHA-p65
T26796 1888-1889 , denotes ,
T26797 1890-1897 NNP denotes pRc/CMV
T26798 1897-1899 CD denotes -3
T26799 1899-1908 NN denotes xHA-p65ΔC
T26800 1909-1910 -LRB- denotes (
T26801 1910-1911 CD denotes 1
T26802 1912-1915 CD denotes 318
T26803 1915-1916 -RRB- denotes )
T26804 1916-1917 , denotes ,
T26805 1918-1920 CC denotes or
T26806 1921-1928 NNP denotes pRc/CMV
T26807 1928-1930 CD denotes -3
T26808 1930-1939 NNP denotes xHA-p65ΔN
T26809 1940-1941 -LRB- denotes (
T26810 1941-1944 CD denotes 122
T26811 1945-1948 CD denotes 551
T26812 1948-1949 -RRB- denotes )
T26813 1950-1951 -LRB- denotes (
T26814 1951-1952 CD denotes 5
T26815 1953-1955 NN denotes µg
T26816 1955-1956 -RRB- denotes )
T26817 1956-1957 . denotes .
T26818 1958-1967 JJ denotes Wild-type
T26819 1968-1971 CC denotes and
T26820 1972-1978 JJ denotes mutant
T26821 1979-1985 NN denotes HA-p65
T26822 1986-1994 NNS denotes proteins
T26823 1995-1999 VBD denotes were
T26824 2000-2018 VBN denotes immunoprecipitated
T26825 2019-2023 IN denotes with
T26826 2024-2031 JJ denotes anti-HA
T26827 2032-2040 NN denotes antibody
T26828 2040-2041 : denotes ;
T26829 2042-2057 NNS denotes immunocomplexes
T26830 2058-2062 VBD denotes were
T26831 2063-2072 VBN denotes separated
T26832 2073-2075 IN denotes by
T26833 2076-2078 CD denotes 12
T26834 2078-2079 NN denotes %
T26835 2080-2083 NNP denotes SDS
T26836 2084-2088 NNP denotes PAGE
T26837 2089-2092 CC denotes and
T26838 2093-2101 VBN denotes analysed
T26839 2102-2104 IN denotes by
T26840 2105-2112 JJ denotes western
T26841 2113-2121 VBG denotes blotting
T26842 2122-2126 IN denotes with
T26843 2127-2136 JJ denotes anti-FLAG
T26844 2137-2139 CC denotes or
T26845 2140-2147 JJ denotes anti-HA
T26846 2148-2158 NNS denotes antibodies
T26847 2158-2159 . denotes .
T26848 2160-2161 -LRB- denotes (
T26849 2161-2162 NNP denotes E
T26850 2162-2163 -RRB- denotes )
T26851 2164-2175 JJ denotes Recombinant
T26852 2176-2179 NNS denotes p65
T26853 2180-2187 NN denotes protein
T26854 2188-2189 -LRB- denotes (
T26855 2189-2192 CD denotes 100
T26856 2193-2195 NN denotes ng
T26857 2195-2196 : denotes ;
T26858 2197-2203 NNP denotes Active
T26859 2204-2209 NNP denotes Motif
T26860 2210-2218 NNP denotes Carlsbad
T26861 2218-2219 , denotes ,
T26862 2220-2222 NNP denotes CA
T26863 2222-2223 , denotes ,
T26864 2224-2227 NNP denotes USA
T26865 2227-2228 -RRB- denotes )
T26866 2229-2232 VBD denotes was
T26867 2233-2235 CD denotes 20
T26868 2236-2239 NN denotes min
T26869 2240-2249 VBN denotes incubated
T26870 2250-2254 IN denotes with
T26871 2255-2266 JJ denotes 32P-labeled
T26872 2267-2272 JJ denotes NF-κB
T26873 2273-2288 JJ denotes double-stranded
T26874 2289-2304 NN denotes oligonucleotide
T26875 2305-2307 IN denotes in
T26876 2308-2316 NN denotes presence
T26877 2317-2319 CC denotes or
T26878 2320-2327 NN denotes absence
T26879 2328-2330 IN denotes of
T26880 2331-2333 IN denotes in
T26881 2334-2339 NN denotes vitro
T26882 2340-2350 VBN denotes translated
T26883 2351-2359 NNP denotes FLAG-Tat
T26884 2360-2362 CC denotes or
T26885 2363-2371 NNP denotes FLAG-Tat
T26886 2372-2373 NNP denotes C
T26887 2373-2374 -LRB- denotes (
T26888 2374-2382 CD denotes 22,25,27
T26889 2382-2383 -RRB- denotes )
T26890 2383-2384 DT denotes A
T26891 2385-2386 -LRB- denotes (
T26892 2386-2387 CD denotes 5
T26893 2388-2390 NN denotes µl
T26894 2390-2391 -RRB- denotes )
T26895 2391-2392 : denotes ;
T26896 2393-2405 NNS denotes competitions
T26897 2406-2410 VBD denotes were
T26898 2411-2420 VBN denotes performed
T26899 2421-2425 IN denotes with
T26900 2426-2430 CD denotes 1.25
T26901 2430-2431 : denotes -
T26902 2432-2434 RP denotes up
T26903 2435-2437 TO denotes to
T26904 2438-2445 JJ denotes 40-fold
T26905 2446-2451 NN denotes molar
T26906 2452-2458 NN denotes excess
T26907 2459-2461 IN denotes of
T26908 2462-2471 JJ denotes unlabeled
T26909 2472-2487 NN denotes oligonucleotide
T26910 2487-2488 . denotes .
T26911 2489-2500 NNP denotes DNA/protein
T26912 2501-2510 NNS denotes complexes
T26913 2511-2515 VBD denotes were
T26914 2516-2525 VBN denotes separated
T26915 2526-2528 IN denotes by
T26916 2529-2530 CD denotes 6
T26917 2530-2531 NN denotes %
T26918 2532-2536 NN denotes PAGE
T26919 2537-2539 IN denotes in
T26920 2540-2543 CD denotes 0.5
T26921 2544-2545 CD denotes ×
T26922 2546-2549 NNP denotes TBE
T26923 2550-2556 NN denotes buffer
T26924 2557-2560 CC denotes and
T26925 2561-2569 VBN denotes analysed
T26926 2570-2572 IN denotes by
T26927 2573-2588 NN denotes autoradiography
T26928 2588-2589 . denotes .
T26929 2590-2591 -LRB- denotes (
T26930 2591-2592 NN denotes F
T26931 2592-2593 -RRB- denotes )
T26932 2594-2606 NN denotes Densitometry
T26933 2607-2609 IN denotes of
T26934 2610-2621 NNS denotes band-shifts
T26935 2622-2627 VBN denotes shown
T26936 2628-2630 IN denotes in
T26937 2631-2633 NNP denotes E.
R11150 T13857 T13858 nsubj Tat,increases
R11151 T13858 T13858 ROOT increases,increases
R11152 T13859 T13861 det the,affinity
R11153 T13860 T13861 nummod p65,affinity
R11154 T13861 T13858 dobj affinity,increases
R11155 T13862 T13861 amod binding,affinity
R11156 T13863 T13862 prep to,binding
R11157 T13864 T13863 pobj DNA,to
R11158 T13865 T13858 prep through,increases
R11159 T13866 T13865 pobj association,through
R11160 T13867 T13858 prep with,increases
R11161 T13868 T13867 pobj p65,with
R11162 T13869 T13870 nsubj We,tested
R11163 T13870 T13858 conj tested,increases
R11164 T13871 T13874 mark whether,interacted
R11165 T13872 T13874 nsubj Tat,interacted
R11166 T13873 T13874 advmod physically,interacted
R11167 T13874 T13870 ccomp interacted,tested
R11168 T13875 T13874 prep with,interacted
R11169 T13876 T13875 pobj p65,with
R11170 T13877 T13870 punct .,tested
R11171 T13878 T13879 compound HeLa,cells
R11172 T13879 T13881 nsubjpass cells,transfected
R11173 T13880 T13881 auxpass were,transfected
R11174 T13881 T13881 ROOT transfected,transfected
R11175 T13882 T13881 prep with,transfected
R11176 T13883 T13885 det the,vectors
R11177 T13884 T13885 compound expression,vectors
R11178 T13885 T13882 pobj vectors,with
R11179 T13886 T13885 prep of,vectors
R11180 T13887 T13886 pobj HA-p65,of
R11181 T13888 T13887 cc and,HA-p65
R11182 T13889 T13887 conj FLAG-Tat,HA-p65
R11183 T13890 T13889 punct ",",FLAG-Tat
R11184 T13891 T13885 cc and,vectors
R11185 T13892 T13893 nummod 24,h
R11186 T13893 T13895 compound h,Tat
R11187 T13894 T13895 amod later,Tat
R11188 T13895 T13885 conj Tat,vectors
R11189 T13896 T13897 auxpass was,immunoprecipitated
R11190 T13897 T13881 conj immunoprecipitated,transfected
R11191 T13898 T13897 prep from,immunoprecipitated
R11192 T13899 T13900 compound cell,extracts
R11193 T13900 T13898 pobj extracts,from
R11194 T13901 T13881 punct .,transfected
R11195 T13902 T13904 det The,protein
R11196 T13903 T13904 nummod p65,protein
R11197 T13904 T13906 nsubjpass protein,coimmunoprecipitated
R11198 T13905 T13906 auxpass was,coimmunoprecipitated
R11199 T13906 T13906 ROOT coimmunoprecipitated,coimmunoprecipitated
R11200 T13907 T13906 prep with,coimmunoprecipitated
R11201 T13908 T13909 amod wild-type,Tat
R11202 T13909 T13907 pobj Tat,with
R11203 T13910 T13909 cc or,Tat
R11204 T13911 T13912 det the,mutants
R11205 T13912 T13909 conj mutants,Tat
R11206 T13913 T13914 compound Tat,T
R11207 T13914 T13914 ROOT T,T
R11208 T13915 T13914 punct ",",T
R11209 T13916 T13914 conj N,T
R11210 T13917 T13916 punct (,N
R11211 T13918 T13916 parataxis "23,24",N
R11212 T13919 T13916 punct ),N
R11213 T13920 T13927 det A,A
R11214 T13921 T13927 punct ",",A
R11215 T13922 T13923 compound Tat,K
R11216 T13923 T13927 nmod K,A
R11217 T13924 T13923 punct (,K
R11218 T13925 T13923 appos "50,51",K
R11219 T13926 T13927 punct ),A
R11278 T13996 T13994 conj N,T
R11279 T13997 T13996 punct (,N
R11280 T13998 T13996 appos "23,24",N
R11281 T13999 T13996 punct ),N
R11282 T14000 T14007 det A,A
R11283 T14001 T14007 punct ",",A
R11284 T14002 T14003 compound Tat,K
R11285 T14003 T14007 nmod K,A
R11286 T14004 T14003 punct (,K
R11287 T14005 T14003 appos "50,51",K
R11288 T14006 T14007 punct ),A
R11289 T14007 T14007 ROOT A,A
R11290 T14008 T14007 cc and,A
R11291 T14009 T14010 compound Tat,R
R11292 T14010 T14007 conj R,A
R11293 T14011 T14010 punct (,R
R11294 T14012 T14010 appos 49,R
R11295 T14013 T14010 appos 57,R
R11296 T14014 T14010 punct ),R
R11297 T14015 T14015 ROOT A,A
R11298 T14016 T14015 punct ",",A
R11299 T14017 T14015 cc and,A
R11300 T14018 T14020 nsubj lack,binding
R11301 T14019 T14020 advmod of,binding
R11302 T14020 T14015 conj binding,A
R11303 T14021 T14020 prep to,binding
R11304 T14022 T14023 compound Tat,C
R11305 T14023 T14021 pobj C,to
R11306 T14024 T14023 punct (,C
R11307 T14025 T14023 appos "22,25,27",C
R11308 T14026 T14023 punct ),C
R11309 T14027 T14030 det A,4B
R11310 T14028 T14030 punct (,4B
R11311 T14029 T14030 compound Figure,4B
R11312 T14030 T14030 ROOT 4B,4B
R11313 T14031 T14030 punct ),4B
R11314 T14032 T14030 punct .,4B
R11315 T14033 T14034 det These,results
R11316 T14034 T14035 nsubj results,indicated
R11317 T14035 T14035 ROOT indicated,indicated
R11318 T14036 T14038 mark that,associated
R11339 T14057 T14058 aux To,identify
R11340 T14058 T14071 advcl identify,transfected
R11341 T14059 T14061 det the,domain
R11342 T14060 T14061 compound p65,domain
R11343 T14061 T14058 dobj domain,identify
R11344 T14062 T14061 acl required,domain
R11345 T14063 T14062 prep for,required
R11346 T14064 T14071 advcl binding,transfected
R11347 T14065 T14064 prep to,binding
R11348 T14066 T14065 pobj Tat,to
R11349 T14067 T14071 punct ",",transfected
R11350 T14068 T14069 compound HeLa,cells
R11351 T14069 T14071 nsubjpass cells,transfected
R11352 T14070 T14071 auxpass were,transfected
R11353 T14071 T14071 ROOT transfected,transfected
R11354 T14072 T14071 prep with,transfected
R11355 T14073 T14072 pobj FLAG-Tat,with
R11356 T14074 T14075 advmod together,with
R11357 T14075 T14073 prep with,FLAG-Tat
R11358 T14076 T14077 amod HA-p65,mutants
R11359 T14077 T14075 pobj mutants,with
R11360 T14078 T14077 punct ",",mutants
R11361 T14079 T14081 nsubjpass which,deleted
R11362 T14080 T14081 auxpass were,deleted
R11363 T14081 T14077 relcl deleted,mutants
R11364 T14082 T14081 prep of,deleted
R11365 T14083 T14085 det the,domain
R11366 T14084 T14085 compound transactivation,domain
R11367 T14085 T14082 pobj domain,of
R11368 T14086 T14087 compound [,p65ΔC
R11369 T14087 T14085 appos p65ΔC,domain
R11370 T14088 T14089 punct (,1
R11371 T14089 T14087 parataxis 1,p65ΔC
R11372 T14090 T14085 appos 318,domain
R11373 T14091 T14071 punct ),transfected
R11374 T14092 T14092 ROOT ],]
R11375 T14093 T14092 punct ",",]
R11376 T14094 T14092 cc or,]
R11377 T14095 T14098 det the,p65ΔN
R11378 T14096 T14098 compound RHD,p65ΔN
R11379 T14097 T14098 compound [,p65ΔN
R11380 T14098 T14092 conj p65ΔN,]
R11381 T14099 T14098 punct (,p65ΔN
R11382 T14100 T14101 dep 122,551
R11383 T14101 T14098 appos 551,p65ΔN
R11384 T14102 T14098 punct ),p65ΔN
R11385 T14103 T14103 ROOT ],]
R11386 T14104 T14106 punct (,4C
R11387 T14105 T14106 compound Figure,4C
R11388 T14106 T14103 appos 4C,]
R11389 T14107 T14106 punct ),4C
R11390 T14108 T14103 punct .,]
R11391 T14109 T14110 nsubj Tat,coimmunoprecipitated
R11392 T14110 T14110 ROOT coimmunoprecipitated,coimmunoprecipitated
R11393 T14111 T14110 prep with,coimmunoprecipitated
R11394 T14112 T14111 pobj p65ΔC,with
R11395 T14113 T14114 punct (,1
R11396 T14114 T14112 nummod 1,p65ΔC
R11405 T14123 T14124 dep 122,551
R11406 T14124 T14121 appos 551,p65ΔN
R11407 T14125 T14121 punct ),p65ΔN
R11408 T14126 T14127 punct (,Figure
R11409 T14127 T14120 appos Figure,with
R11410 T14128 T14127 nummod 4D,Figure
R11411 T14129 T14127 punct ),Figure
R11412 T14130 T14131 punct ",",indicating
R11413 T14131 T14110 conj indicating,coimmunoprecipitated
R11414 T14132 T14138 mark that,involved
R11415 T14133 T14134 det the,RHD
R11416 T14134 T14138 nsubjpass RHD,involved
R11417 T14135 T14134 prep of,RHD
R11418 T14136 T14135 pobj p65,of
R11419 T14137 T14138 auxpass was,involved
R11420 T14138 T14131 ccomp involved,indicating
R11421 T14139 T14138 prep in,involved
R11422 T14140 T14142 det the,interaction
R11423 T14141 T14142 amod physical,interaction
R11424 T14142 T14139 pobj interaction,in
R11425 T14143 T14142 prep with,interaction
R11426 T14144 T14143 pobj Tat,with
R11427 T14145 T14110 punct .,coimmunoprecipitated
R11428 T14146 T14147 aux To,address
R11429 T14147 T14158 advcl address,purified
R11430 T14148 T14149 det the,effect
R11431 T14149 T14147 dobj effect,address
R11432 T14150 T14149 prep of,effect
R11433 T14151 T14150 pobj Tat,of
R11441 T14159 T14161 amod recombinant,protein
R11442 T14160 T14161 compound p65,protein
R11443 T14161 T14163 nsubjpass protein,incubated
R11444 T14162 T14163 auxpass was,incubated
R11445 T14163 T14163 ROOT incubated,incubated
R11446 T14164 T14163 prep with,incubated
R11447 T14165 T14167 amod 32P-labeled-NF-κB,oligonucleotide
R11448 T14166 T14167 amod double-stranded,oligonucleotide
R11449 T14167 T14164 pobj oligonucleotide,with
R11450 T14168 T14167 prep in,oligonucleotide
R11451 T14169 T14168 pobj presence,in
R11452 T14170 T14169 cc or,presence
R11453 T14171 T14169 conj absence,presence
R11454 T14172 T14171 prep of,absence
R11455 T14173 T14174 amod wild-type,Tat
R11456 T14174 T14172 pobj Tat,of
R11457 T14175 T14174 cc or,Tat
R11458 T14176 T14177 compound Tat,C
R11459 T14177 T14174 conj C,Tat
R11460 T14178 T14177 punct (,C
R11461 T14179 T14177 appos "22,25,27",C
R11462 T14180 T14177 punct ),C
R11463 T14181 T14187 nsubjpass A,analysed
R11464 T14182 T14181 punct ",",A
R11465 T14183 T14181 cc and,A
R11466 T14184 T14185 nummod p65,DNA-binding
R11467 T14185 T14186 nsubj DNA-binding,was
R11491 T14209 T14206 punct ),C
R11492 T14210 T14219 intj A,was
R11493 T14211 T14219 punct ",",was
R11494 T14212 T14219 advcl lacking,was
R11495 T14213 T14215 det the,site
R11496 T14214 T14215 amod binding,site
R11497 T14215 T14212 dobj site,lacking
R11498 T14216 T14215 prep for,site
R11499 T14217 T14216 pobj p65,for
R11500 T14218 T14219 punct ",",was
R11501 T14219 T14219 ROOT was,was
R11502 T14220 T14219 acomp ineffective,was
R11503 T14221 T14222 punct (,Figure
R11504 T14222 T14219 npadvmod Figure,was
R11505 T14223 T14222 nummod 4E,Figure
R11506 T14224 T14222 punct ),Figure
R11507 T14225 T14219 punct .,was
R11508 T14226 T14227 det The,p65
R11509 T14227 T14228 nsubj p65,binding
R11510 T14228 T14232 nsubjpass binding,evaluated
R11511 T14229 T14228 prep to,binding
R11512 T14230 T14229 pobj DNA,to
R11513 T14231 T14232 auxpass was,evaluated
R11514 T14232 T14232 ROOT evaluated,evaluated
R11515 T14233 T14232 agent by,evaluated
R11516 T14234 T14233 pobj competition,by
R11517 T14235 T14234 prep with,competition
R11518 T14236 T14237 amod increasing,amounts
R11519 T14237 T14235 pobj amounts,with
R11520 T14238 T14237 prep of,amounts
R11521 T14239 T14240 amod cold,competitor
R11522 T14240 T14242 compound competitor,oligonucleotide
R11523 T14241 T14242 compound NF-κB,oligonucleotide
R11524 T14242 T14238 pobj oligonucleotide,of
R11525 T14243 T14242 punct ",",oligonucleotide
R11526 T14244 T14245 nsubj which,showed
R11527 T14245 T14242 relcl showed,oligonucleotide
R11528 T14246 T14249 mark that,enhanced
R11529 T14247 T14249 nsubj Tat,enhanced
R11530 T14248 T14249 advmod significantly,enhanced
R11531 T14249 T14245 ccomp enhanced,showed
R11532 T14250 T14253 det the,binding
R11533 T14251 T14252 nummod p65,affinity
R11544 T14262 T14232 punct .,evaluated
R20925 T26585 T26586 compound Tat,associates
R20926 T26586 T26586 ROOT associates,associates
R20927 T26587 T26586 prep with,associates
R20928 T26588 T26589 nsubj p65,increasing
R20929 T26589 T26587 pcomp increasing,with
R20930 T26590 T26592 poss its,binding
R20931 T26591 T26592 advmod DNA-affinity,binding
R20932 T26592 T26589 dobj binding,increasing
R20933 T26593 T26586 punct .,associates
R20934 T26594 T26600 punct (,transfected
R20935 T26595 T26598 nmod A,cells
R20936 T26596 T26595 punct ),A
R20937 T26597 T26598 compound HeLa,cells
R20938 T26598 T26600 nsubjpass cells,transfected
R20939 T26599 T26600 auxpass were,transfected
R20940 T26600 T26600 ROOT transfected,transfected
R20941 T26601 T26600 prep with,transfected
R20942 T26602 T26601 pobj pRc/CMV,with
R20943 T26603 T26604 nummod -3,xHA-p65
R20944 T26604 T26600 npadvmod xHA-p65,transfected
R20945 T26605 T26607 punct (,µg
R20946 T26606 T26607 nummod 5,µg
R20947 T26607 T26604 appos µg,xHA-p65
R20948 T26608 T26607 punct ),µg
R20949 T26609 T26604 prep in,xHA-p65
R20950 T26610 T26609 pobj presence,in
R20951 T26611 T26610 cc or,presence
R20952 T26612 T26610 conj absence,presence
R20953 T26613 T26612 prep of,absence
R20954 T26614 T26613 pobj p3xFLAG-Tat,of
R20955 T26615 T26612 punct ",",absence
R20956 T26616 T26617 compound p3xFLAG-Tat,T
R20957 T26617 T26604 conj T,xHA-p65
R20958 T26618 T26617 punct ",",T
R20959 T26619 T26617 conj N,T
R20960 T26620 T26621 punct (,"23,24"
R20961 T26621 T26619 parataxis "23,24",N
R20962 T26622 T26621 punct ),"23,24"
R20963 T26623 T26626 det A,K
R20964 T26624 T26626 punct ",",K
R20965 T26625 T26626 compound p3xFLAG-Tat,K
R20966 T26626 T26604 npadvmod K,xHA-p65
R20967 T26627 T26626 punct (,K
R20968 T26628 T26626 appos "50,51",K
R20969 T26629 T26626 punct ),K
R20970 T26630 T26633 det A,R
R20971 T26631 T26633 punct ",",R
R20972 T26632 T26633 amod p3xFLAG-Tat,R
R20973 T26633 T26633 ROOT R,R
R20974 T26634 T26633 punct (,R
R20975 T26635 T26633 appos 49,R
R20976 T26636 T26633 appos 57,R
R20977 T26637 T26633 punct ),R
R20978 T26638 T26638 ROOT A,A
R20979 T26639 T26638 punct ",",A
R20980 T26640 T26638 cc or,A
R20981 T26641 T26642 compound p3xFLAG-Tat,C
R20982 T26642 T26638 conj C,A
R20983 T26643 T26642 punct (,C
R20984 T26644 T26642 appos "22,25,27",C
R20985 T26645 T26642 punct ),C
R20986 T26646 T26649 det A,µg
R20987 T26647 T26649 punct (,µg
R20988 T26648 T26649 nummod 5,µg
R20989 T26649 T26649 ROOT µg,µg
R20990 T26650 T26649 punct ),µg
R20991 T26651 T26649 punct .,µg
R20992 T26652 T26653 amod FLAG-Tat,proteins
R20993 T26653 T26655 nsubjpass proteins,immunoprecipitated
R20994 T26654 T26655 auxpass were,immunoprecipitated
R20995 T26655 T26662 ccomp immunoprecipitated,separated
R20996 T26656 T26655 prep with,immunoprecipitated
R20997 T26657 T26658 amod anti-FLAG,antibody
R20998 T26658 T26656 pobj antibody,with
R20999 T26659 T26662 punct ;,separated
R21000 T26660 T26662 nsubjpass immunocomplexes,separated
R21001 T26661 T26662 auxpass were,separated
R21002 T26662 T26662 ROOT separated,separated
R21003 T26663 T26662 prep by,separated
R21004 T26664 T26665 nummod 12,%
R21005 T26665 T26666 compound %,SDS
R21006 T26666 T26663 pobj SDS,by
R21007 T26667 T26666 conj PAGE,SDS
R21008 T26668 T26662 cc and,separated
R21009 T26669 T26662 conj analysed,separated
R21010 T26670 T26669 agent by,analysed
R21011 T26671 T26672 amod western,blotting
R21012 T26672 T26670 pobj blotting,by
R21013 T26673 T26672 prep with,blotting
R21014 T26674 T26677 amod anti-FLAG,antibodies
R21015 T26675 T26674 cc or,anti-FLAG
R21016 T26676 T26674 conj anti-HA,anti-FLAG
R21017 T26677 T26673 pobj antibodies,with
R21018 T26678 T26662 punct .,separated
R21019 T26679 T26680 punct (,B
R21020 T26680 T26680 ROOT B,B
R21021 T26681 T26680 punct ),B
R21022 T26682 T26691 prep In,incubated
R21023 T26683 T26685 amod vitro,p65
R21024 T26684 T26685 amod translated,p65
R21025 T26685 T26682 pobj p65,In
R21026 T26686 T26685 punct (,p65
R21027 T26687 T26688 nummod 5,µl
R21028 T26688 T26685 appos µl,p65
R21029 T26689 T26688 punct ),µl
R21030 T26690 T26691 auxpass was,incubated
R21031 T26691 T26691 ROOT incubated,incubated
R21032 T26692 T26691 prep with,incubated
R21033 T26693 T26692 pobj GST-Tat,with
R21034 T26694 T26693 punct ",",GST-Tat
R21035 T26695 T26696 compound GST-Tat,T
R21036 T26696 T26693 conj T,GST-Tat
R21037 T26697 T26696 punct ",",T
R21038 T26698 T26696 conj N,T
R21039 T26699 T26700 punct (,"23,24"
R21040 T26700 T26698 parataxis "23,24",N
R21041 T26701 T26700 punct ),"23,24"
R21042 T26702 T26705 det A,K
R21043 T26703 T26705 punct ",",K
R21044 T26704 T26705 compound GST-Tat,K
R21045 T26705 T26705 ROOT K,K
R21046 T26706 T26705 punct (,K
R21047 T26707 T26705 appos "50,51",K
R21048 T26708 T26705 punct ),K
R21049 T26709 T26712 det A,R
R21050 T26710 T26712 punct ",",R
R21051 T26711 T26712 compound GST-Tat,R
R21052 T26712 T26712 ROOT R,R
R21053 T26713 T26712 punct (,R
R21054 T26714 T26712 appos 49,R
R21055 T26715 T26712 appos 57,R
R21056 T26716 T26712 punct ),R
R21057 T26717 T26720 det A,C
R21058 T26718 T26720 punct ",",C
R21059 T26719 T26720 compound GST-Tat,C
R21060 T26720 T26720 ROOT C,C
R21061 T26721 T26720 punct (,C
R21062 T26722 T26720 appos "22,25,27",C
R21063 T26723 T26720 punct ),C
R21064 T26724 T26724 ROOT A,A
R21065 T26725 T26724 punct ",",A
R21066 T26726 T26724 cc or,A
R21067 T26727 T26724 conj GST,A
R21068 T26728 T26730 punct (,µg
R21069 T26729 T26730 nummod 5,µg
R21070 T26730 T26727 appos µg,GST
R21071 T26731 T26730 punct ),µg
R21072 T26732 T26727 amod conjugated,GST
R21073 T26733 T26732 prep with,conjugated
R21074 T26734 T26733 pobj Glutathione-Sepharose,with
R21075 T26735 T26727 punct .,GST
R21076 T26736 T26737 compound Protein,complexes
R21077 T26737 T26739 nsubjpass complexes,recovered
R21078 T26738 T26739 auxpass were,recovered
R21079 T26739 T26739 ROOT recovered,recovered
R21080 T26740 T26739 agent by,recovered
R21081 T26741 T26740 pobj GST-pull,by
R21082 T26742 T26739 advmod down,recovered
R21083 T26743 T26739 punct ",",recovered
R21084 T26744 T26739 conj separated,recovered
R21085 T26745 T26744 prep by,separated
R21086 T26746 T26747 nummod 12,%
R21087 T26747 T26748 compound %,SDS
R21088 T26748 T26745 pobj SDS,by
R21089 T26749 T26748 conj PAGE,SDS
R21090 T26750 T26748 punct ",",SDS
R21091 T26751 T26744 cc and,separated
R21092 T26752 T26744 conj analysed,separated
R21093 T26753 T26752 agent by,analysed
R21094 T26754 T26755 amod western,blotting
R21095 T26755 T26753 pobj blotting,by
R21096 T26756 T26755 prep with,blotting
R21097 T26757 T26760 amod anti-p65,antibodies
R21098 T26758 T26757 cc or,anti-p65
R21099 T26759 T26757 conj anti-GST,anti-p65
R21100 T26760 T26756 pobj antibodies,with
R21101 T26761 T26739 punct .,recovered
R21102 T26762 T26763 punct (,C
R21103 T26763 T26766 nmod C,representation
R21104 T26764 T26763 punct ),C
R21105 T26765 T26766 amod Schematic,representation
R21106 T26766 T26766 ROOT representation,representation
R21107 T26767 T26766 prep of,representation
R21108 T26768 T26772 amod wild-type,proteins
R21109 T26769 T26768 cc and,wild-type
R21110 T26770 T26768 conj mutant,wild-type
R21111 T26771 T26772 compound p65,proteins
R21112 T26772 T26767 pobj proteins,of
R21113 T26773 T26766 punct .,representation
R21114 T26774 T26780 punct (,transfected
R21115 T26775 T26778 compound D,cells
R21116 T26776 T26777 punct ),HeLa
R21117 T26777 T26778 compound HeLa,cells
R21118 T26778 T26780 nsubjpass cells,transfected
R21119 T26779 T26780 auxpass were,transfected
R21120 T26780 T26780 ROOT transfected,transfected
R21121 T26781 T26780 prep with,transfected
R21122 T26782 T26781 pobj p3xFLAG-Tat,with
R21123 T26783 T26785 punct (,µg
R21124 T26784 T26785 nummod 5,µg
R21125 T26785 T26782 appos µg,p3xFLAG-Tat
R21126 T26786 T26785 punct ),µg
R21127 T26787 T26780 prep in,transfected
R21128 T26788 T26789 det the,presence
R21129 T26789 T26787 pobj presence,in
R21130 T26790 T26789 cc or,presence
R21131 T26791 T26789 conj absence,presence
R21132 T26792 T26791 prep of,absence
R21133 T26793 T26795 nmod pRc/CMV,xHA-p65
R21134 T26794 T26795 nummod -3,xHA-p65
R21135 T26795 T26792 pobj xHA-p65,of
R21136 T26796 T26795 punct ",",xHA-p65
R21137 T26797 T26799 nmod pRc/CMV,xHA-p65ΔC
R21138 T26798 T26799 nummod -3,xHA-p65ΔC
R21139 T26799 T26795 appos xHA-p65ΔC,xHA-p65
R21140 T26800 T26801 punct (,1
R21141 T26801 T26808 nummod 1,xHA-p65ΔN
R21142 T26802 T26801 appos 318,1
R21143 T26803 T26801 punct ),1
R21144 T26804 T26801 punct ",",1
R21145 T26805 T26801 cc or,1
R21146 T26806 T26808 nmod pRc/CMV,xHA-p65ΔN
R21147 T26807 T26808 nummod -3,xHA-p65ΔN
R21148 T26808 T26799 appos xHA-p65ΔN,xHA-p65ΔC
R21149 T26809 T26808 punct (,xHA-p65ΔN
R21150 T26810 T26811 dep 122,551
R21151 T26811 T26808 appos 551,xHA-p65ΔN
R21152 T26812 T26808 punct ),xHA-p65ΔN
R21153 T26813 T26815 punct (,µg
R21154 T26814 T26815 nummod 5,µg
R21155 T26815 T26808 appos µg,xHA-p65ΔN
R21156 T26816 T26815 punct ),µg
R21157 T26817 T26780 punct .,transfected
R21158 T26818 T26822 amod Wild-type,proteins
R21159 T26819 T26818 cc and,Wild-type
R21160 T26820 T26818 conj mutant,Wild-type
R21161 T26821 T26822 compound HA-p65,proteins
R21162 T26822 T26824 nsubjpass proteins,immunoprecipitated
R21163 T26823 T26824 auxpass were,immunoprecipitated
R21164 T26824 T26831 ccomp immunoprecipitated,separated
R21165 T26825 T26824 prep with,immunoprecipitated
R21166 T26826 T26827 amod anti-HA,antibody
R21167 T26827 T26825 pobj antibody,with
R21168 T26828 T26831 punct ;,separated
R21169 T26829 T26831 nsubjpass immunocomplexes,separated
R21170 T26830 T26831 auxpass were,separated
R21171 T26831 T26831 ROOT separated,separated
R21172 T26832 T26831 prep by,separated
R21173 T26833 T26834 nummod 12,%
R21174 T26834 T26832 pobj %,by
R21175 T26835 T26836 compound SDS,PAGE
R21176 T26836 T26831 npadvmod PAGE,separated
R21177 T26837 T26836 cc and,PAGE
R21178 T26838 T26836 conj analysed,PAGE
R21179 T26839 T26838 agent by,analysed
R21180 T26840 T26841 amod western,blotting
R21181 T26841 T26839 pobj blotting,by
R21182 T26842 T26841 prep with,blotting
R21183 T26843 T26846 amod anti-FLAG,antibodies
R21184 T26844 T26843 cc or,anti-FLAG
R21185 T26845 T26843 conj anti-HA,anti-FLAG
R21186 T26846 T26842 pobj antibodies,with
R21187 T26847 T26831 punct .,separated
R21188 T26848 T26866 punct (,was
R21189 T26849 T26853 nmod E,protein
R21190 T26850 T26849 punct ),E
R21191 T26851 T26852 amod Recombinant,p65
R21192 T26852 T26853 compound p65,protein
R21193 T26853 T26866 ccomp protein,was
R21194 T26854 T26856 punct (,ng
R21195 T26855 T26856 nummod 100,ng
R21196 T26856 T26853 appos ng,protein
R21197 T26857 T26866 punct ;,was
R21198 T26858 T26860 compound Active,Carlsbad
R21199 T26859 T26860 compound Motif,Carlsbad
R21200 T26860 T26866 nsubj Carlsbad,was
R21201 T26861 T26860 punct ",",Carlsbad
R21202 T26862 T26860 conj CA,Carlsbad
R21203 T26863 T26862 punct ",",CA
R21204 T26864 T26862 conj USA,CA
R21205 T26865 T26862 punct ),CA
R21206 T26866 T26866 ROOT was,was
R21207 T26867 T26868 nummod 20,min
R21208 T26868 T26866 attr min,was
R21209 T26869 T26868 acl incubated,min
R21210 T26870 T26869 prep with,incubated
R21211 T26871 T26874 amod 32P-labeled,oligonucleotide
R21212 T26872 T26874 amod NF-κB,oligonucleotide
R21213 T26873 T26874 amod double-stranded,oligonucleotide
R21214 T26874 T26870 pobj oligonucleotide,with
R21215 T26875 T26874 prep in,oligonucleotide
R21216 T26876 T26875 pobj presence,in
R21217 T26877 T26876 cc or,presence
R21218 T26878 T26876 conj absence,presence
R21219 T26879 T26876 prep of,presence
R21220 T26880 T26882 mark in,translated
R21221 T26881 T26882 nsubj vitro,translated
R21222 T26882 T26869 advcl translated,incubated
R21223 T26883 T26882 dobj FLAG-Tat,translated
R21224 T26884 T26883 cc or,FLAG-Tat
R21225 T26885 T26886 compound FLAG-Tat,C
R21226 T26886 T26883 conj C,FLAG-Tat
R21227 T26887 T26886 punct (,C
R21228 T26888 T26886 appos "22,25,27",C
R21229 T26889 T26886 punct ),C
R21230 T26890 T26893 det A,µl
R21231 T26891 T26893 punct (,µl
R21232 T26892 T26893 nummod 5,µl
R21233 T26893 T26893 ROOT µl,µl
R21234 T26894 T26893 punct ),µl
R21235 T26895 T26898 punct ;,performed
R21236 T26896 T26898 nsubjpass competitions,performed
R21237 T26897 T26898 auxpass were,performed
R21238 T26898 T26898 ROOT performed,performed
R21239 T26899 T26898 prep with,performed
R21240 T26900 T26906 nummod 1.25,excess
R21241 T26901 T26902 punct -,up
R21242 T26902 T26906 advmod up,excess
R21243 T26903 T26906 quantmod to,excess
R21244 T26904 T26903 pobj 40-fold,to
R21245 T26905 T26906 compound molar,excess
R21246 T26906 T26899 pobj excess,with
R21247 T26907 T26906 prep of,excess
R21248 T26908 T26909 amod unlabeled,oligonucleotide
R21249 T26909 T26907 pobj oligonucleotide,of
R21250 T26910 T26898 punct .,performed
R21251 T26911 T26912 compound DNA/protein,complexes
R21252 T26912 T26914 nsubjpass complexes,separated
R21253 T26913 T26914 auxpass were,separated
R21254 T26914 T26914 ROOT separated,separated
R21255 T26915 T26914 prep by,separated
R21256 T26916 T26917 nummod 6,%
R21257 T26917 T26918 compound %,PAGE
R21258 T26918 T26915 pobj PAGE,by
R21259 T26919 T26914 prep in,separated
R21260 T26920 T26923 nummod 0.5,buffer
R21261 T26921 T26923 nummod ×,buffer
R21262 T26922 T26923 compound TBE,buffer
R21263 T26923 T26919 pobj buffer,in
R21264 T26924 T26923 cc and,buffer
R21265 T26925 T26914 advcl analysed,separated
R21266 T26926 T26925 agent by,analysed
R21267 T26927 T26926 pobj autoradiography,by
R21268 T26928 T26914 punct .,separated
R21269 T26929 T26932 punct (,Densitometry
R21270 T26930 T26932 nmod F,Densitometry
R21271 T26931 T26932 punct ),Densitometry
R21272 T26932 T26932 ROOT Densitometry,Densitometry
R21273 T26933 T26932 prep of,Densitometry
R21274 T26934 T26933 pobj band-shifts,of
R21275 T26935 T26932 acl shown,Densitometry
R21276 T26936 T26935 prep in,shown
R21277 T26937 T26936 pobj E.,in
R11220 T13927 T13927 ROOT A,A
R11221 T13928 T13927 cc and,A
R11222 T13929 T13930 compound Tat,R
R11223 T13930 T13927 conj R,A
R11224 T13942 T13930 punct (,R
R11225 T13943 T13930 appos 49,R
R11226 T13944 T13930 appos 57,R
R11227 T13945 T13930 punct ),R
R11228 T13946 T13946 ROOT A,A
R11229 T13947 T13946 prep at,A
R11230 T13948 T13949 amod similar,levels
R11231 T13949 T13947 pobj levels,at
R11232 T13950 T13946 punct ",",A
R11233 T13951 T13958 mark while,observed
R11234 T13952 T13954 det a,decrease
R11235 T13953 T13954 amod significant,decrease
R11236 T13954 T13958 nsubjpass decrease,observed
R11237 T13955 T13954 prep in,decrease
R11238 T13956 T13955 pobj coimmunoprecipitation,in
R11239 T13957 T13958 auxpass was,observed
R11240 T13958 T13946 advcl observed,A
R11241 T13959 T13958 prep for,observed
R11242 T13960 T13961 compound Tat,C
R11243 T13961 T13959 pobj C,for
R11244 T13962 T13961 punct (,C
R11245 T13963 T13961 appos "22,25,27",C
R11246 T13964 T13961 punct ),C
R11247 T13965 T13968 det A,4A
R11248 T13966 T13968 punct (,4A
R11249 T13967 T13968 nmod Figure,4A
R11250 T13968 T13968 ROOT 4A,4A
R11251 T13969 T13968 punct ),4A
R11252 T13970 T13968 punct .,4A
R11253 T13971 T13980 advmod Consistently,showed
R11254 T13972 T13980 punct ",",showed
R11255 T13973 T13980 nsubj GST-pull,showed
R11256 T13974 T13973 prt down,GST-pull
R11257 T13975 T13973 prep of,GST-pull
R11258 T13976 T13980 prep in,showed
R11259 T13977 T13979 amod vitro,proteins
R11260 T13978 T13979 amod translated,proteins
R11261 T13979 T13976 pobj proteins,in
R11262 T13980 T13980 ROOT showed,showed
R11263 T13981 T13983 det the,binding
R11264 T13982 T13983 amod direct,binding
R11265 T13983 T13980 dobj binding,showed
R11266 T13984 T13983 prep of,binding
R11267 T13985 T13984 pobj p65,of
R11268 T13986 T13987 aux to,wild-type
R11269 T13987 T13983 relcl wild-type,binding
R11270 T13988 T13987 dobj Tat,wild-type
R11271 T13989 T13987 punct ",",wild-type
R11272 T13990 T13983 cc or,binding
R11273 T13991 T13992 det the,mutants
R11274 T13992 T13994 nsubj mutants,T
R11275 T13993 T13994 compound Tat,T
R11276 T13994 T13983 conj T,binding
R11277 T13995 T13994 punct ",",T
R11319 T14037 T14038 nsubj Tat,associated
R11320 T14038 T14035 ccomp associated,indicated
R11321 T14039 T14038 prep with,associated
R11322 T14040 T14039 pobj p65,with
R11323 T14041 T14040 prep through,p65
R11324 T14042 T14044 det the,domain
R11325 T14043 T14044 compound cysteine-rich,domain
R11326 T14044 T14041 pobj domain,through
R11327 T14045 T14044 cc and,domain
R11328 T14046 T14044 conj ruled-out,domain
R11329 T14047 T14048 det the,requirement
R11330 T14048 T14044 conj requirement,domain
R11331 T14049 T14048 prep of,requirement
R11332 T14050 T14051 amod bridging,proteins
R11333 T14051 T14049 pobj proteins,of
R11334 T14052 T14053 aux to,mediate
R11335 T14053 T14048 acl mediate,requirement
R11336 T14054 T14055 amod such,interaction
R11337 T14055 T14053 dobj interaction,mediate
R11338 T14056 T14035 punct .,indicated
R11397 T14115 T14112 appos 318,p65ΔC
R11398 T14116 T14112 punct ),p65ΔC
R11399 T14117 T14110 punct ",",coimmunoprecipitated
R11400 T14118 T14110 cc and,coimmunoprecipitated
R11401 T14119 T14120 neg not,with
R11402 T14120 T14131 prep with,indicating
R11403 T14121 T14120 pobj p65ΔN,with
R11404 T14122 T14121 punct (,p65ΔN
R11434 T14152 T14149 prep on,effect
R11435 T14153 T14156 det the,affinity
R11436 T14154 T14156 nummod p65,affinity
R11437 T14155 T14156 amod DNA-binding,affinity
R11438 T14156 T14152 pobj affinity,on
R11439 T14157 T14158 punct ",",purified
R11440 T14158 T14161 amod purified,protein
R11468 T14186 T14187 auxpass was,analysed
R11469 T14187 T14187 ROOT analysed,analysed
R11470 T14188 T14187 agent by,analysed
R11471 T14189 T14188 pobj EMSA,by
R11472 T14190 T14187 punct .,analysed
R11473 T14191 T14195 mark Whereas,increased
R11474 T14192 T14193 compound wild-type,Tat
R11475 T14193 T14195 nsubj Tat,increased
R11476 T14194 T14195 advmod significantly,increased
R11477 T14195 T14195 ROOT increased,increased
R11478 T14196 T14197 det the,binding
R11479 T14197 T14195 dobj binding,increased
R11480 T14198 T14197 prep of,binding
R11481 T14199 T14198 pobj p65,of
R11482 T14200 T14195 prep to,increased
R11483 T14201 T14200 pobj DNA,to
R11484 T14202 T14201 punct ",",DNA
R11485 T14203 T14206 det the,C
R11486 T14204 T14206 amod mutant,C
R11487 T14205 T14206 compound Tat,C
R11488 T14206 T14201 appos C,DNA
R11489 T14207 T14206 punct (,C
R11490 T14208 T14206 appos "22,25,27",C
R11534 T14252 T14253 nsubj affinity,binding
R11535 T14253 T14249 ccomp binding,enhanced
R11536 T14254 T14253 prep to,binding
R11537 T14255 T14254 pobj DNA,to
R11538 T14256 T14255 punct (,DNA
R11539 T14257 T14258 compound Figure,4E
R11540 T14258 T14255 appos 4E,DNA
R11541 T14259 T14258 cc and,4E
R11542 T14260 T14258 conj F,4E
R11543 T14261 T14255 punct ),DNA

GO-BP

Id Subject Object Predicate Lexical cue
T14263 555-574 http://purl.obolibrary.org/obo/GO_0006412 denotes translated proteins
T14264 2788-2803 http://purl.obolibrary.org/obo/GO_2000144 denotes transactivation
T26938 1386-1396 http://purl.obolibrary.org/obo/GO_0006412 denotes translated
T26939 2340-2350 http://purl.obolibrary.org/obo/GO_0006412 denotes translated
T26940 2220-2222 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-MF

Id Subject Object Predicate Lexical cue
T14265 31-38 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14266 593-600 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14267 703-710 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14268 2674-2681 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14269 3083-3090 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14270 3276-3283 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14271 3356-3363 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14272 3420-3427 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14273 3479-3486 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14274 3651-3658 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T14275 31-45 http://purl.obolibrary.org/obo/GO_0003677 denotes binding to DNA
T14276 3479-3493 http://purl.obolibrary.org/obo/GO_0003677 denotes binding to DNA
T14277 3651-3665 http://purl.obolibrary.org/obo/GO_0003677 denotes binding to DNA
T26941 972-979 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T26942 1245-1253 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T26943 2032-2040 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T26944 2148-2158 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T26945 2220-2222 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-CC

Id Subject Object Predicate Lexical cue
T14278 134-139 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14279 2695-2700 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26946 990-995 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26947 1795-1800 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26948 1245-1253 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T26949 2032-2040 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T26950 2148-2158 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T26951 1245-1253 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T26952 2032-2040 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T26953 2148-2158 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T26954 1570-1587 http://purl.obolibrary.org/obo/GO_0043234 denotes Protein complexes
T26955 2493-2510 http://purl.obolibrary.org/obo/GO_0043234 denotes protein complexes
T26956 2489-2510 http://purl.obolibrary.org/obo/GO_0001114 denotes DNA/protein complexes
T26957 2489-2510 http://purl.obolibrary.org/obo/GO_0032993 denotes DNA/protein complexes
T26958 2489-2510 http://purl.obolibrary.org/obo/GO_0097522 denotes DNA/protein complexes

sentences

Id Subject Object Predicate Lexical cue
T13324 0-74 Sentence denotes Tat increases the p65 affinity binding to DNA through association with p65
T13325 75-128 Sentence denotes We tested whether Tat physically interacted with p65.
T13326 129-270 Sentence denotes HeLa cells were transfected with the expression vectors of HA-p65 and FLAG-Tat, and 24 h later Tat was immunoprecipitated from cell extracts.
T13327 271-514 Sentence denotes The p65 protein was coimmunoprecipitated with wild-type Tat or the mutants Tat T,N(23,24)A, Tat K(50,51)A and Tat R(49–57)A at similar levels, while a significant decrease in coimmunoprecipitation was observed for Tat C(22,25,27)A (Figure 4A).
T13328 515-743 Sentence denotes Consistently, GST-pull down of in vitro translated proteins showed the direct binding of p65 to wild-type Tat, or the mutants Tat T,N(23,24)A, Tat K(50,51)A and Tat R(49–57)A, and lack of binding to Tat C(22,25,27)A (Figure 4B).
T13329 744-909 Sentence denotes These results indicated that Tat associated with p65 through the cysteine-rich domain and ruled-out the requirement of bridging proteins to mediate such interaction.
T13330 2634-2869 Sentence denotes To identify the p65 domain required for binding to Tat, HeLa cells were transfected with FLAG-Tat together with HA-p65 mutants, which were deleted of the transactivation domain [p65ΔC (1–318)], or the RHD [p65ΔN (122–551)] (Figure 4C).
T13331 2870-3038 Sentence denotes Tat coimmunoprecipitated with p65ΔC (1–318), and not with p65ΔN (122–551) (Figure 4D), indicating that the RHD of p65 was involved in the physical interaction with Tat.
T13332 3039-3305 Sentence denotes To address the effect of Tat on the p65 DNA-binding affinity, purified recombinant p65 protein was incubated with 32P-labeled-NF-κB double-stranded oligonucleotide in presence or absence of wild-type Tat or Tat C(22,25,27)A, and p65 DNA-binding was analysed by EMSA.
T13333 3306-3470 Sentence denotes Whereas wild-type Tat significantly increased the binding of p65 to DNA, the mutant Tat C(22,25,27)A, lacking the binding site for p65, was ineffective (Figure 4E).
T13334 3471-3684 Sentence denotes The p65 binding to DNA was evaluated by competition with increasing amounts of cold competitor NF-κB oligonucleotide, which showed that Tat significantly enhanced the p65 affinity binding to DNA (Figure 4E and F).
T26499 920-1187 Sentence denotes Tat associates with p65 increasing its DNA-affinity binding. (A) HeLa cells were transfected with pRc/CMV-3xHA-p65 (5 µg) in presence or absence of p3xFLAG-Tat, p3xFLAG-Tat T,N(23,24)A, p3xFLAG-Tat K(50,51)A, p3xFLAG-Tat R(49–57)A, or p3xFLAG-Tat C(22,25,27)A (5 µg).
T26500 1188-1957 Sentence denotes FLAG-Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (B) In vitro translated p65 (5 µl) was incubated with GST-Tat, GST-Tat T,N(23,24)A, GST-Tat K(50,51)A, GST-Tat R(49–57)A, GST-Tat C(22,25,27)A, or GST (5 µg) conjugated with Glutathione-Sepharose. Protein complexes were recovered by GST-pull down, separated by 12% SDS–PAGE, and analysed by western blotting with anti-p65 or anti-GST antibodies. (C) Schematic representation of wild-type and mutant p65 proteins. (D) HeLa cells were transfected with p3xFLAG-Tat (5 µg) in the presence or absence of pRc/CMV-3xHA-p65, pRc/CMV-3xHA-p65ΔC (1–318), or pRc/CMV-3xHA-p65ΔN (122–551) (5 µg).
T26501 1958-2488 Sentence denotes Wild-type and mutant HA-p65 proteins were immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (E) Recombinant p65 protein (100 ng; Active Motif Carlsbad, CA, USA) was 20 min incubated with 32P-labeled NF-κB double-stranded oligonucleotide in presence or absence of in vitro translated FLAG-Tat or FLAG-Tat C(22,25,27)A (5 µl); competitions were performed with 1.25- up to 40-fold molar excess of unlabeled oligonucleotide.
T26502 2489-2633 Sentence denotes DNA/protein complexes were separated by 6% PAGE in 0.5 × TBE buffer and analysed by autoradiography. (F) Densitometry of band-shifts shown in E.
T165 0-74 Sentence denotes Tat increases the p65 affinity binding to DNA through association with p65
T166 75-128 Sentence denotes We tested whether Tat physically interacted with p65.
T167 129-270 Sentence denotes HeLa cells were transfected with the expression vectors of HA-p65 and FLAG-Tat, and 24 h later Tat was immunoprecipitated from cell extracts.
T168 271-514 Sentence denotes The p65 protein was coimmunoprecipitated with wild-type Tat or the mutants Tat T,N(23,24)A, Tat K(50,51)A and Tat R(49–57)A at similar levels, while a significant decrease in coimmunoprecipitation was observed for Tat C(22,25,27)A (Figure 4A).
T169 515-743 Sentence denotes Consistently, GST-pull down of in vitro translated proteins showed the direct binding of p65 to wild-type Tat, or the mutants Tat T,N(23,24)A, Tat K(50,51)A and Tat R(49–57)A, and lack of binding to Tat C(22,25,27)A (Figure 4B).
T170 744-909 Sentence denotes These results indicated that Tat associated with p65 through the cysteine-rich domain and ruled-out the requirement of bridging proteins to mediate such interaction.
T171 910-919 Sentence denotes Figure 4.
T172 920-1187 Sentence denotes Tat associates with p65 increasing its DNA-affinity binding. (A) HeLa cells were transfected with pRc/CMV-3xHA-p65 (5 µg) in presence or absence of p3xFLAG-Tat, p3xFLAG-Tat T,N(23,24)A, p3xFLAG-Tat K(50,51)A, p3xFLAG-Tat R(49–57)A, or p3xFLAG-Tat C(22,25,27)A (5 µg).
T173 1188-1569 Sentence denotes FLAG-Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (B) In vitro translated p65 (5 µl) was incubated with GST-Tat, GST-Tat T,N(23,24)A, GST-Tat K(50,51)A, GST-Tat R(49–57)A, GST-Tat C(22,25,27)A, or GST (5 µg) conjugated with Glutathione-Sepharose.
T174 1570-1957 Sentence denotes Protein complexes were recovered by GST-pull down, separated by 12% SDS–PAGE, and analysed by western blotting with anti-p65 or anti-GST antibodies. (C) Schematic representation of wild-type and mutant p65 proteins. (D) HeLa cells were transfected with p3xFLAG-Tat (5 µg) in the presence or absence of pRc/CMV-3xHA-p65, pRc/CMV-3xHA-p65ΔC (1–318), or pRc/CMV-3xHA-p65ΔN (122–551) (5 µg).
T175 1958-2488 Sentence denotes Wild-type and mutant HA-p65 proteins were immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-FLAG or anti-HA antibodies. (E) Recombinant p65 protein (100 ng; Active Motif Carlsbad, CA, USA) was 20 min incubated with 32P-labeled NF-κB double-stranded oligonucleotide in presence or absence of in vitro translated FLAG-Tat or FLAG-Tat C(22,25,27)A (5 µl); competitions were performed with 1.25- up to 40-fold molar excess of unlabeled oligonucleotide.
T176 2489-2633 Sentence denotes DNA/protein complexes were separated by 6% PAGE in 0.5 × TBE buffer and analysed by autoradiography. (F) Densitometry of band-shifts shown in E.
T177 2634-2869 Sentence denotes To identify the p65 domain required for binding to Tat, HeLa cells were transfected with FLAG-Tat together with HA-p65 mutants, which were deleted of the transactivation domain [p65ΔC (1–318)], or the RHD [p65ΔN (122–551)] (Figure 4C).
T178 2870-3038 Sentence denotes Tat coimmunoprecipitated with p65ΔC (1–318), and not with p65ΔN (122–551) (Figure 4D), indicating that the RHD of p65 was involved in the physical interaction with Tat.
T179 3039-3305 Sentence denotes To address the effect of Tat on the p65 DNA-binding affinity, purified recombinant p65 protein was incubated with 32P-labeled-NF-κB double-stranded oligonucleotide in presence or absence of wild-type Tat or Tat C(22,25,27)A, and p65 DNA-binding was analysed by EMSA.
T180 3306-3470 Sentence denotes Whereas wild-type Tat significantly increased the binding of p65 to DNA, the mutant Tat C(22,25,27)A, lacking the binding site for p65, was ineffective (Figure 4E).
T181 3471-3684 Sentence denotes The p65 binding to DNA was evaluated by competition with increasing amounts of cold competitor NF-κB oligonucleotide, which showed that Tat significantly enhanced the p65 affinity binding to DNA (Figure 4E and F).

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T14678 0-3 Protein denotes Tat
T14679 4-13 Positive_regulation denotes increases
T14680 18-21 Protein denotes p65
T14681 31-38 Binding denotes binding
T14682 54-65 Binding denotes association
T14683 71-74 Protein denotes p65
T14684 93-96 Protein denotes Tat
T14685 108-118 Binding denotes interacted true
T14686 124-127 Protein denotes p65
T14687 188-194 Protein denotes HA-p65
T14688 199-207 Protein denotes FLAG-Tat
T14689 224-227 Protein denotes Tat
T14690 232-250 Gene_expression denotes immunoprecipitated
T14691 275-278 Protein denotes p65
T14692 291-311 Binding denotes coimmunoprecipitated
T14693 327-330 Protein denotes Tat
T14694 346-361 Protein denotes Tat T,N(23,24)A
T14695 363-376 Protein denotes Tat K(50,51)A
T14696 381-394 Protein denotes Tat R(49–57)A
T14697 434-442 Negative_regulation denotes decrease
T14698 446-467 Binding denotes coimmunoprecipitation
T14699 485-501 Protein denotes Tat C(22,25,27)A
T14700 529-532 Protein denotes GST
T14701 593-600 Binding denotes binding
T14702 604-607 Protein denotes p65
T14703 621-624 Protein denotes Tat
T14704 641-656 Protein denotes Tat T,N(23,24)A
T14705 658-671 Protein denotes Tat K(50,51)A
T14706 676-689 Protein denotes Tat R(49–57)A
T14707 703-710 Binding denotes binding true
T14708 714-730 Protein denotes Tat C(22,25,27)A
T14709 773-776 Protein denotes Tat
T14710 777-787 Binding denotes associated
T14711 793-796 Protein denotes p65
T14712 809-829 Entity denotes cysteine-rich domain
T14713 884-891 Positive_regulation denotes mediate true
T14714 2650-2653 Protein denotes p65
T14715 2654-2660 Entity denotes domain
T14716 2661-2669 Positive_regulation denotes required true
T14717 2674-2681 Binding denotes binding
T14718 2685-2688 Protein denotes Tat
T14719 2723-2731 Protein denotes FLAG-Tat
T14720 2746-2752 Protein denotes HA-p65
T14721 2773-2780 Negative_regulation denotes deleted
T14722 2773-2780 Negative_regulation denotes deleted
T14723 2788-2810 Entity denotes transactivation domain
T14724 2812-2825 Protein denotes p65ΔC (1–318)
T14725 2835-2838 Entity denotes RHD
T14726 2840-2855 Protein denotes p65ΔN (122–551)
T14727 2870-2873 Protein denotes Tat
T14728 2874-2894 Binding denotes coimmunoprecipitated true
T14729 2900-2913 Protein denotes p65ΔC (1–318)
T14730 2928-2943 Protein denotes p65ΔN (122–551)
T14731 2977-2980 Entity denotes RHD
T14732 2984-2987 Protein denotes p65
T14733 3017-3028 Binding denotes interaction
T14734 3034-3037 Protein denotes Tat
T14735 3054-3060 Regulation denotes effect true
T14736 3064-3067 Protein denotes Tat
T14737 3075-3078 Protein denotes p65
T14738 3083-3090 Binding denotes binding
T14739 3122-3125 Protein denotes p65
T14740 3239-3242 Protein denotes Tat
T14741 3246-3262 Protein denotes Tat C(22,25,27)A
T14742 3268-3271 Protein denotes p65
T14743 3276-3283 Binding denotes binding true
T14744 3324-3327 Protein denotes Tat
T14745 3342-3351 Positive_regulation denotes increased
T14746 3356-3363 Binding denotes binding
T14747 3367-3370 Protein denotes p65
T14748 3390-3406 Protein denotes Tat C(22,25,27)A
T14749 3408-3415 Negative_regulation denotes lacking
T14750 3420-3427 Binding denotes binding
T14751 3428-3432 Entity denotes site
T14752 3437-3440 Protein denotes p65
T14753 3446-3457 Positive_regulation denotes ineffective true
T14754 3475-3478 Protein denotes p65
T14755 3479-3486 Binding denotes binding true
T14756 3607-3610 Protein denotes Tat
T14757 3625-3633 Positive_regulation denotes enhanced
T14758 3638-3641 Protein denotes p65
T14759 3651-3658 Binding denotes binding
T27104 920-923 Protein denotes Tat
T27105 924-934 Binding denotes associates
T27106 940-943 Protein denotes p65
T27107 944-954 Positive_regulation denotes increasing
T27108 972-979 Binding denotes binding
T27109 1026-1034 Protein denotes 3xHA-p65
T27110 1076-1079 Protein denotes Tat
T27111 1089-1104 Protein denotes Tat T,N(23,24)A
T27112 1114-1127 Protein denotes Tat K(50,51)A
T27113 1137-1150 Protein denotes Tat R(49–57)A
T27114 1163-1179 Protein denotes Tat C(22,25,27)A
T27115 1188-1196 Protein denotes FLAG-Tat
T27116 1397-1400 Protein denotes p65
T27117 1427-1434 Protein denotes GST-Tat
T27118 1436-1455 Protein denotes GST-Tat T,N(23,24)A
T27119 1457-1474 Protein denotes GST-Tat K(50,51)A
T27120 1476-1493 Protein denotes GST-Tat R(49–57)A
T27121 1495-1515 Protein denotes GST-Tat C(22,25,27)A
T27122 1520-1523 Protein denotes GST
T27123 1606-1609 Protein denotes GST
T27124 1772-1775 Protein denotes p65
T27125 1831-1834 Protein denotes Tat
T27126 1880-1888 Protein denotes 3xHA-p65
T27127 1898-1908 Protein denotes 3xHA-p65ΔC
T27128 1929-1939 Protein denotes 3xHA-p65ΔN
T27129 1979-1985 Protein denotes HA-p65
T27130 2176-2179 Protein denotes p65
T27131 2351-2359 Protein denotes FLAG-Tat
T27132 2363-2384 Protein denotes FLAG-Tat C(22,25,27)A
R11791 T14678 T14682 themeOf Tat,association
R11792 T14680 T14681 themeOf p65,binding
R11793 T14681 T14679 themeOf binding,increases
R11794 T14682 T14679 causeOf association,increases
R11795 T14683 T14682 themeOf p65,association
R11796 T14684 T14685 themeOf Tat,interacted
R11797 T14686 T14685 themeOf p65,interacted
R11798 T14689 T14690 themeOf Tat,immunoprecipitated
R11799 T14691 T14692 themeOf p65,coimmunoprecipitated
R11800 T14691 T14698 themeOf p65,coimmunoprecipitation
R11801 T14693 T14692 themeOf Tat,coimmunoprecipitated
R11802 T14698 T14697 themeOf coimmunoprecipitation,decrease
R11803 T14699 T14697 causeOf "Tat C(22,25,27)A",decrease
R11804 T14699 T14698 themeOf "Tat C(22,25,27)A",coimmunoprecipitation
R11805 T14702 T14701 themeOf p65,binding
R11806 T14702 T14707 themeOf p65,binding
R11807 T14703 T14701 themeOf Tat,binding
R11808 T14708 T14707 themeOf "Tat C(22,25,27)A",binding
R11809 T14710 T14713 themeOf associated,mediate
R11810 T14711 T14710 themeOf p65,associated
R11811 T14712 T14710 themeOf cysteine-rich domain,associated
R11812 T14712 T14709 partOf cysteine-rich domain,Tat
R11813 T14714 T14717 themeOf p65,binding
R11814 T14715 T14716 causeOf domain,required
R11815 T14715 T14714 partOf domain,p65
R11816 T14717 T14716 themeOf binding,required
R11817 T14718 T14717 themeOf Tat,binding
R11818 T14723 T14722 themeOf transactivation domain,deleted
R11819 T14723 T14714 partOf transactivation domain,p65
R11820 T14725 T14721 themeOf RHD,deleted
R11821 T14725 T14714 partOf RHD,p65
R11822 T14727 T14728 themeOf Tat,coimmunoprecipitated
R11823 T14729 T14728 themeOf p65ΔC (1–318),coimmunoprecipitated
R11824 T14731 T14733 themeOf RHD,interaction
R11825 T14731 T14732 partOf RHD,p65
R11826 T14734 T14733 themeOf Tat,interaction
R11827 T14736 T14735 causeOf Tat,effect
R11828 T14737 T14738 themeOf p65,binding
R11829 T14738 T14735 themeOf binding,effect
R11830 T14742 T14743 themeOf p65,binding
R11831 T14744 T14745 causeOf Tat,increased
R11832 T14746 T14745 themeOf binding,increased
R11833 T14746 T14753 themeOf binding,ineffective
R11834 T14747 T14746 themeOf p65,binding
R11835 T14748 T14753 causeOf "Tat C(22,25,27)A",ineffective
R11836 T14751 T14749 themeOf site,lacking
R11837 T14751 T14744 partOf site,Tat
R11838 T14751 T14750 themeOf site,binding
R11839 T14752 T14750 themeOf p65,binding
R11840 T14754 T14755 themeOf p65,binding
R11841 T14756 T14757 causeOf Tat,enhanced
R11842 T14758 T14759 themeOf p65,binding
R11843 T14759 T14757 themeOf binding,enhanced
R21302 T27104 T27105 themeOf Tat,associates
R21303 T27105 T27107 causeOf associates,increasing
R21304 T27106 T27105 themeOf p65,associates
R21305 T27106 T27108 themeOf p65,binding
R21306 T27108 T27107 themeOf binding,increasing

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T14760 0-3 Protein denotes Tat
T14761 18-21 Protein denotes p65
T14762 31-38 Binding denotes binding
T14763 4-13 Positive_regulation denotes increases
T14764 93-96 Protein denotes Tat
T14765 124-127 Protein denotes p65
T14766 108-118 Binding denotes interacted
T14767 188-190 Protein denotes HA
T14768 191-194 Protein denotes p65
T14769 199-203 Protein denotes FLAG
T14770 204-207 Protein denotes Tat
T14771 224-227 Protein denotes Tat
T14772 166-176 Gene_expression denotes expression
T14773 166-176 Gene_expression denotes expression
T14774 166-176 Gene_expression denotes expression
T14775 166-176 Gene_expression denotes expression
T14776 275-286 Protein denotes p65 protein
T14777 317-330 Protein denotes wild-type Tat
T14778 338-351 Protein denotes mutants Tat T
T14779 485-490 Protein denotes Tat C
T14780 291-311 Binding denotes coimmunoprecipitated
T14781 291-311 Binding denotes coimmunoprecipitated
T14782 434-442 Negative_regulation denotes decrease
T14783 434-442 Negative_regulation denotes decrease
T14784 529-532 Protein denotes GST
T14785 604-607 Protein denotes p65
T14786 633-646 Protein denotes mutants Tat T
T14787 714-719 Protein denotes Tat C
T14788 593-600 Binding denotes binding
T14789 593-600 Binding denotes binding
T14790 703-710 Binding denotes binding
T14791 703-710 Binding denotes binding
T14792 703-710 Binding denotes binding
T14793 773-776 Protein denotes Tat
T14794 793-796 Protein denotes p65
T14795 777-787 Binding denotes associated
T14796 897-908 Binding denotes interaction
T14797 2650-2660 Protein denotes p65 domain
T14798 2685-2688 Protein denotes Tat
T14799 2746-2760 Protein denotes HA-p65 mutants
T14800 2674-2681 Binding denotes binding
T14801 2661-2669 Positive_regulation denotes required
T14802 2984-2987 Protein denotes p65
T14803 3034-3037 Protein denotes Tat
T14804 3017-3028 Binding denotes interaction
T14805 3064-3067 Protein denotes Tat
T14806 3075-3078 Protein denotes p65
T14807 3110-3133 Protein denotes recombinant p65 protein
T14808 3168-3170 Protein denotes κB
T14809 3246-3251 Protein denotes Tat C
T14810 3268-3271 Protein denotes p65
T14811 3367-3370 Protein denotes p65
T14812 3437-3440 Protein denotes p65
T14813 3356-3363 Binding denotes binding
T14814 3420-3427 Binding denotes binding
T14815 3342-3351 Positive_regulation denotes increased
T14816 3475-3478 Protein denotes p65
T14817 3550-3571 Protein denotes cold competitor NF-κB
T14818 3607-3610 Protein denotes Tat
T14819 3638-3641 Protein denotes p65
T14820 3479-3486 Binding denotes binding
T14821 3651-3658 Binding denotes binding
T14822 3625-3633 Positive_regulation denotes enhanced
T27133 920-923 Protein denotes Tat
T27134 940-943 Protein denotes p65
T27135 924-934 Binding denotes associates
T27136 1068-1079 Protein denotes p3xFLAG-Tat
T27137 1081-1088 Protein denotes p3xFLAG
T27138 1089-1092 Protein denotes Tat
T27139 1188-1205 Protein denotes FLAG-Tat proteins
T27140 1427-1430 Protein denotes GST
T27141 1431-1434 Protein denotes Tat
T27142 1436-1439 Protein denotes GST
T27143 1440-1443 Protein denotes Tat
T27144 1476-1479 Protein denotes GST
T27145 1480-1483 Protein denotes Tat
T27146 1606-1609 Protein denotes GST
T27147 1691-1694 Protein denotes p65
T27148 1698-1717 Protein denotes anti-GST antibodies
T27149 1765-1784 Protein denotes mutant p65 proteins
T27150 1972-1994 Protein denotes mutant HA-p65 proteins
T27151 2024-2040 Protein denotes anti-HA antibody
T27152 2000-2018 Binding denotes immunoprecipitated
T27153 2000-2018 Binding denotes immunoprecipitated
T27154 2164-2187 Protein denotes Recombinant p65 protein
T27155 2267-2272 Protein denotes NF-κB
T27156 2351-2355 Protein denotes FLAG
T27157 2356-2359 Protein denotes Tat
T27158 2363-2367 Protein denotes FLAG
T27159 2368-2371 Protein denotes Tat
R11844 T14760 T14763 causeOf Tat,increases
R11845 T14761 T14762 themeOf p65,binding
R11846 T14762 T14763 themeOf binding,increases
R11847 T14764 T14766 themeOf Tat,interacted
R11848 T14765 T14766 themeOf p65,interacted
R11849 T14767 T14772 themeOf HA,expression
R11850 T14768 T14773 themeOf p65,expression
R11851 T14769 T14774 themeOf FLAG,expression
R11852 T14770 T14775 themeOf Tat,expression
R11853 T14776 T14781 themeOf p65 protein,coimmunoprecipitated
R11854 T14777 T14780 themeOf wild-type Tat,coimmunoprecipitated
R11855 T14777 T14781 themeOf wild-type Tat,coimmunoprecipitated
R11856 T14780 T14782 themeOf coimmunoprecipitated,decrease
R11857 T14781 T14783 themeOf coimmunoprecipitated,decrease
R11858 T14785 T14788 themeOf p65,binding
R11859 T14785 T14789 themeOf p65,binding
R11860 T14786 T14789 themeOf mutants Tat T,binding
R11861 T14786 T14790 themeOf mutants Tat T,binding
R11862 T14786 T14792 themeOf mutants Tat T,binding
R11863 T14787 T14791 themeOf Tat C,binding
R11864 T14787 T14792 themeOf Tat C,binding
R11865 T14793 T14795 themeOf Tat,associated
R11866 T14793 T14796 themeOf Tat,interaction
R11867 T14794 T14795 themeOf p65,associated
R11868 T14797 T14800 themeOf p65 domain,binding
R11869 T14798 T14800 themeOf Tat,binding
R11870 T14800 T14801 themeOf binding,required
R11871 T14802 T14804 themeOf p65,interaction
R11872 T14803 T14804 themeOf Tat,interaction
R11873 T14811 T14813 themeOf p65,binding
R11874 T14812 T14814 themeOf p65,binding
R11875 T14813 T14815 themeOf binding,increased
R11876 T14816 T14820 themeOf p65,binding
R11877 T14818 T14822 causeOf Tat,enhanced
R11878 T14819 T14821 themeOf p65,binding
R11879 T14821 T14822 themeOf binding,enhanced
R21307 T27133 T27135 themeOf Tat,associates
R21308 T27150 T27153 themeOf mutant HA-p65 proteins,immunoprecipitated
R21309 T27151 T27152 themeOf anti-HA antibody,immunoprecipitated
R21310 T27151 T27153 themeOf anti-HA antibody,immunoprecipitated

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T13162 0-3 Protein denotes Tat
T13163 4-13 Positive_regulation denotes increases
T13164 18-21 Protein denotes p65
T13165 31-38 Binding denotes binding true
T13166 54-65 Binding denotes association
T13167 71-74 Protein denotes p65
T13168 93-96 Protein denotes Tat
T13169 108-118 Binding denotes interacted
T13170 124-127 Protein denotes p65
T13171 166-176 Gene_expression denotes expression true
T13172 188-194 Protein denotes HA-p65
T13173 199-207 Protein denotes FLAG-Tat true
T13174 224-227 Protein denotes Tat true
T13175 275-278 Protein denotes p65
T13176 291-311 Binding denotes coimmunoprecipitated
T13177 327-330 Protein denotes Tat
T13178 346-361 Protein denotes Tat T,N(23,24)A true
T13179 363-376 Protein denotes Tat K(50,51)A
T13180 381-394 Protein denotes Tat R(49–57)A true
T13181 434-442 Negative_regulation denotes decrease
T13182 485-501 Protein denotes Tat C(22,25,27)A true
T13183 529-532 Protein denotes GST
T13184 593-600 Binding denotes binding
T13185 604-607 Protein denotes p65
T13186 621-624 Protein denotes Tat
T13187 641-656 Protein denotes Tat T,N(23,24)A true
T13188 658-671 Protein denotes Tat K(50,51)A true
T13189 676-689 Protein denotes Tat R(49–57)A
T13190 695-699 Negative_regulation denotes lack
T13191 703-710 Binding denotes binding
T13192 714-730 Protein denotes Tat C(22,25,27)A
T13193 773-776 Protein denotes Tat
T13194 777-787 Binding denotes associated
T13195 793-796 Protein denotes p65
T13196 809-829 Entity denotes cysteine-rich domain
T13197 897-908 Binding denotes interaction
T13198 2650-2653 Protein denotes p65
T13199 2654-2660 Entity denotes domain
T13200 2661-2669 Positive_regulation denotes required
T13201 2674-2681 Binding denotes binding
T13202 2685-2688 Protein denotes Tat
T13203 2706-2717 Gene_expression denotes transfected
T13204 2723-2731 Protein denotes FLAG-Tat
T13205 2746-2752 Protein denotes HA-p65
T13206 2773-2780 Negative_regulation denotes deleted
T13207 2788-2810 Entity denotes transactivation domain
T13208 2812-2827 Protein denotes p65ΔC (1–318)],
T13209 2835-2838 Entity denotes RHD
T13210 2840-2856 Protein denotes p65ΔN (122–551)]
T13211 2870-2873 Protein denotes Tat
T13212 2874-2894 Binding denotes coimmunoprecipitated
T13213 2900-2914 Protein denotes p65ΔC (1–318),
T13214 2928-2943 Protein denotes p65ΔN (122–551)
T13215 2977-2980 Entity denotes RHD
T13216 2984-2987 Protein denotes p65
T13217 3017-3028 Binding denotes interaction
T13218 3034-3037 Protein denotes Tat
T13219 3054-3060 Regulation denotes effect
T13220 3064-3067 Protein denotes Tat
T13221 3075-3078 Protein denotes p65
T13222 3083-3090 Binding denotes binding
T13223 3122-3125 Protein denotes p65
T13224 3239-3242 Protein denotes Tat
T13225 3246-3262 Protein denotes Tat C(22,25,27)A
T13226 3268-3271 Protein denotes p65
T13227 3276-3283 Binding denotes binding
T13228 3324-3327 Protein denotes Tat
T13229 3342-3351 Positive_regulation denotes increased
T13230 3356-3363 Binding denotes binding
T13231 3367-3370 Protein denotes p65
T13232 3390-3406 Protein denotes Tat C(22,25,27)A
T13233 3408-3415 Negative_regulation denotes lacking
T13234 3420-3427 Binding denotes binding
T13235 3437-3440 Protein denotes p65
T13236 3475-3478 Protein denotes p65
T13237 3479-3486 Binding denotes binding
T13238 3607-3610 Protein denotes Tat
T13239 3625-3633 Positive_regulation denotes enhanced
T13240 3638-3641 Protein denotes p65
T13241 3651-3658 Binding denotes binding
T26440 920-923 Protein denotes Tat
T26441 924-934 Binding denotes associates
T26442 940-943 Protein denotes p65
T26443 944-954 Positive_regulation denotes increasing
T26444 972-979 Binding denotes binding
T26445 1026-1034 Protein denotes 3xHA-p65
T26446 1057-1064 Negative_regulation denotes absence
T26447 1076-1079 Protein denotes Tat
T26448 1089-1104 Protein denotes Tat T,N(23,24)A
T26449 1114-1127 Protein denotes Tat K(50,51)A
T26450 1137-1150 Protein denotes Tat R(49–57)A
T26451 1163-1179 Protein denotes Tat C(22,25,27)A
T26452 1188-1196 Protein denotes FLAG-Tat
T26453 1397-1400 Protein denotes p65
T26454 1427-1434 Protein denotes GST-Tat
T26455 1436-1455 Protein denotes GST-Tat T,N(23,24)A
T26456 1457-1474 Protein denotes GST-Tat K(50,51)A
T26457 1476-1493 Protein denotes GST-Tat R(49–57)A
T26458 1495-1515 Protein denotes GST-Tat C(22,25,27)A
T26459 1520-1523 Protein denotes GST
T26460 1606-1609 Protein denotes GST
T26461 1772-1775 Protein denotes p65
T26462 1831-1834 Protein denotes Tat
T26463 1880-1888 Protein denotes 3xHA-p65
T26464 1898-1908 Protein denotes 3xHA-p65ΔC
T26465 1929-1939 Protein denotes 3xHA-p65ΔN
T26466 1979-1985 Protein denotes HA-p65
T26467 2176-2179 Protein denotes p65
T26468 2351-2359 Protein denotes FLAG-Tat
T26469 2363-2384 Protein denotes FLAG-Tat C(22,25,27)A
R10655 T13162 T13163 causeOf Tat,increases
R10656 T13162 T13166 themeOf Tat,association
R10657 T13164 T13165 themeOf p65,binding
R10658 T13165 T13163 themeOf binding,increases
R10659 T13166 T13163 causeOf association,increases
R10660 T13167 T13166 themeOf p65,association
R10661 T13168 T13169 themeOf Tat,interacted
R10662 T13170 T13169 themeOf p65,interacted
R10663 T13172 T13171 themeOf HA-p65,expression
R10664 T13173 T13171 themeOf FLAG-Tat,expression
R10665 T13174 T13171 themeOf Tat,expression
R10666 T13175 T13176 themeOf p65,coimmunoprecipitated
R10667 T13177 T13176 themeOf Tat,coimmunoprecipitated
R10668 T13182 T13181 causeOf "Tat C(22,25,27)A",decrease
R10669 T13185 T13184 themeOf p65,binding
R10670 T13186 T13184 themeOf Tat,binding
R10671 T13187 T13184 themeOf "Tat T,N(23,24)A",binding
R10672 T13191 T13190 themeOf binding,lack
R10673 T13192 T13191 themeOf "Tat C(22,25,27)A",binding
R10674 T13193 T13197 themeOf Tat,interaction
R10675 T13193 T13194 themeOf Tat,associated
R10676 T13195 T13194 themeOf p65,associated
R10677 T13195 T13197 themeOf p65,interaction
R10678 T13196 T13193 partOf cysteine-rich domain,Tat
R10679 T13196 T13195 partOf cysteine-rich domain,p65
R10680 T13196 T13194 themeOf cysteine-rich domain,associated
R10681 T13198 T13201 themeOf p65,binding
R10682 T13198 T13203 themeOf p65,transfected
R10683 T13199 T13198 partOf domain,p65
R10684 T13199 T13200 causeOf domain,required
R10685 T13201 T13200 themeOf binding,required
R10686 T13202 T13203 themeOf Tat,transfected
R10687 T13202 T13201 themeOf Tat,binding
R10688 T13204 T13203 causeOf FLAG-Tat,transfected
R10689 T13207 T13206 themeOf transactivation domain,deleted
R10690 T13207 T13198 partOf transactivation domain,p65
R10691 T13209 T13198 partOf RHD,p65
R10692 T13209 T13206 themeOf RHD,deleted
R10693 T13211 T13212 themeOf Tat,coimmunoprecipitated
R10694 T13213 T13212 themeOf "p65ΔC (1–318),",coimmunoprecipitated
R10695 T13215 T13217 themeOf RHD,interaction
R10696 T13215 T13216 partOf RHD,p65
R10697 T13218 T13217 themeOf Tat,interaction
R10698 T13220 T13219 causeOf Tat,effect
R10699 T13221 T13222 themeOf p65,binding
R10700 T13222 T13219 themeOf binding,effect
R10701 T13226 T13227 themeOf p65,binding
R10702 T13228 T13229 causeOf Tat,increased
R10703 T13230 T13229 themeOf binding,increased
R10704 T13231 T13230 themeOf p65,binding
R10705 T13235 T13234 themeOf p65,binding
R10706 T13236 T13237 themeOf p65,binding
R10707 T13238 T13239 causeOf Tat,enhanced
R10708 T13240 T13241 themeOf p65,binding
R10709 T13241 T13239 themeOf binding,enhanced
R20914 T26440 T26441 themeOf Tat,associates
R20915 T26440 T26444 themeOf Tat,binding
R20916 T26441 T26443 causeOf associates,increasing
R20917 T26442 T26441 themeOf p65,associates
R20918 T26444 T26443 themeOf binding,increasing

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T13251 188-194 Protein denotes HA-p65
T13252 199-207 Protein denotes FLAG-Tat
T13253 224-227 Protein denotes Tat
T13254 232-250 Gene_expression denotes immunoprecipitated
T13255 275-278 Protein denotes p65
T13256 291-311 Binding denotes coimmunoprecipitated
T13257 327-330 Protein denotes Tat
T13258 346-361 Protein denotes Tat T,N(23,24)A
T13259 363-376 Protein denotes Tat K(50,51)A
T13260 381-394 Protein denotes Tat R(49–57)A
T13261 434-442 Negative_regulation denotes decrease
T13262 446-467 Binding denotes coimmunoprecipitation
T13263 485-501 Protein denotes Tat C(22,25,27)A
T13264 529-532 Protein denotes GST
T13265 593-600 Binding denotes binding
T13266 604-607 Protein denotes p65
T13267 621-624 Protein denotes Tat
T13283 2723-2731 Protein denotes FLAG-Tat
T13284 2746-2752 Protein denotes HA-p65
T13285 2773-2780 Negative_regulation denotes deleted
T13286 2773-2780 Negative_regulation denotes deleted
T13287 2788-2810 Entity denotes transactivation domain
T13288 2812-2825 Protein denotes p65ΔC (1–318)
T13289 2835-2838 Entity denotes RHD
T13290 2840-2855 Protein denotes p65ΔN (122–551)
T13291 2870-2873 Protein denotes Tat
T13292 2874-2894 Binding denotes coimmunoprecipitated true
T13293 2900-2913 Protein denotes p65ΔC (1–318)
T13294 2928-2943 Protein denotes p65ΔN (122–551)
T13295 2977-2980 Entity denotes RHD
T13296 2984-2987 Protein denotes p65
T13297 3017-3028 Binding denotes interaction
T13298 3034-3037 Protein denotes Tat
T13299 3054-3060 Regulation denotes effect true
T13300 3064-3067 Protein denotes Tat
T13301 3075-3078 Protein denotes p65
T13302 3083-3090 Binding denotes binding
T13303 3122-3125 Protein denotes p65
T13304 3239-3242 Protein denotes Tat
T13305 3246-3262 Protein denotes Tat C(22,25,27)A
T13306 3268-3271 Protein denotes p65
T13307 3276-3283 Binding denotes binding true
T13308 3324-3327 Protein denotes Tat
T13309 3342-3351 Positive_regulation denotes increased
T13310 3356-3363 Binding denotes binding
T13311 3367-3370 Protein denotes p65
T13312 3390-3406 Protein denotes Tat C(22,25,27)A
T13313 3408-3415 Negative_regulation denotes lacking
T13314 3420-3427 Binding denotes binding
T13315 3428-3432 Entity denotes site
T13316 3437-3440 Protein denotes p65
T13317 3446-3457 Positive_regulation denotes ineffective true
T13318 3475-3478 Protein denotes p65
T13319 3479-3486 Binding denotes binding true
T13320 3607-3610 Protein denotes Tat
T13321 3625-3633 Positive_regulation denotes enhanced
T13322 3638-3641 Protein denotes p65
T13323 3651-3658 Binding denotes binding
T26497 2351-2359 Protein denotes FLAG-Tat
T26498 2363-2384 Protein denotes FLAG-Tat C(22,25,27)A
T13242 0-3 Protein denotes Tat
T13243 4-13 Positive_regulation denotes increases
T13244 18-21 Protein denotes p65
T13245 31-38 Binding denotes binding
T13246 54-65 Binding denotes association
T13247 71-74 Protein denotes p65
T13248 93-96 Protein denotes Tat
T13249 108-118 Binding denotes interacted true
T13250 124-127 Protein denotes p65
T13268 641-656 Protein denotes Tat T,N(23,24)A
T13269 658-671 Protein denotes Tat K(50,51)A
T13270 676-689 Protein denotes Tat R(49–57)A
T13271 703-710 Binding denotes binding true
T13272 714-730 Protein denotes Tat C(22,25,27)A
T13273 773-776 Protein denotes Tat
T13274 777-787 Binding denotes associated
T13275 793-796 Protein denotes p65
T13276 809-829 Entity denotes cysteine-rich domain
T13277 884-891 Positive_regulation denotes mediate true
T13278 2650-2653 Protein denotes p65
T13279 2654-2660 Entity denotes domain
T13280 2661-2669 Positive_regulation denotes required true
T13281 2674-2681 Binding denotes binding
T13282 2685-2688 Protein denotes Tat
T26470 920-923 Protein denotes Tat
T26471 924-934 Binding denotes associates
T26472 940-943 Protein denotes p65
T26473 944-954 Positive_regulation denotes increasing
T26474 972-979 Binding denotes binding
T26475 1026-1034 Protein denotes 3xHA-p65
T26476 1076-1079 Protein denotes Tat
T26477 1089-1104 Protein denotes Tat T,N(23,24)A
T26478 1114-1127 Protein denotes Tat K(50,51)A
T26479 1137-1150 Protein denotes Tat R(49–57)A
T26480 1163-1179 Protein denotes Tat C(22,25,27)A
T26481 1188-1196 Protein denotes FLAG-Tat
T26482 1397-1400 Protein denotes p65
T26483 1427-1434 Protein denotes GST-Tat
T26484 1436-1455 Protein denotes GST-Tat T,N(23,24)A
T26485 1457-1474 Protein denotes GST-Tat K(50,51)A
T26486 1476-1493 Protein denotes GST-Tat R(49–57)A
T26487 1495-1515 Protein denotes GST-Tat C(22,25,27)A
T26488 1520-1523 Protein denotes GST
T26489 1606-1609 Protein denotes GST
T26490 1772-1775 Protein denotes p65
T26491 1831-1834 Protein denotes Tat
T26492 1880-1888 Protein denotes 3xHA-p65
T26493 1898-1908 Protein denotes 3xHA-p65ΔC
T26494 1929-1939 Protein denotes 3xHA-p65ΔN
T26495 1979-1985 Protein denotes HA-p65
T26496 2176-2179 Protein denotes p65
R10718 T13255 T13256 themeOf p65,coimmunoprecipitated
R10719 T13255 T13262 themeOf p65,coimmunoprecipitation
R10720 T13257 T13256 themeOf Tat,coimmunoprecipitated
R10721 T13262 T13261 themeOf coimmunoprecipitation,decrease
R10722 T13263 T13261 causeOf "Tat C(22,25,27)A",decrease
R10723 T13263 T13262 themeOf "Tat C(22,25,27)A",coimmunoprecipitation
R10724 T13266 T13265 themeOf p65,binding
R10725 T13266 T13271 themeOf p65,binding
R10726 T13267 T13265 themeOf Tat,binding
R10727 T13272 T13271 themeOf "Tat C(22,25,27)A",binding
R10728 T13274 T13277 themeOf associated,mediate
R10729 T13275 T13274 themeOf p65,associated
R10730 T13276 T13274 themeOf cysteine-rich domain,associated
R10731 T13276 T13273 partOf cysteine-rich domain,Tat
R10732 T13278 T13281 themeOf p65,binding
R10733 T13279 T13280 causeOf domain,required
R10734 T13279 T13278 partOf domain,p65
R10735 T13281 T13280 themeOf binding,required
R10736 T13282 T13281 themeOf Tat,binding
R10737 T13287 T13286 themeOf transactivation domain,deleted
R10738 T13287 T13278 partOf transactivation domain,p65
R10739 T13289 T13285 themeOf RHD,deleted
R10740 T13289 T13278 partOf RHD,p65
R10741 T13291 T13292 themeOf Tat,coimmunoprecipitated
R10742 T13293 T13292 themeOf p65ΔC (1–318),coimmunoprecipitated
R10743 T13295 T13297 themeOf RHD,interaction
R10744 T13295 T13296 partOf RHD,p65
R10745 T13298 T13297 themeOf Tat,interaction
R10746 T13300 T13299 causeOf Tat,effect
R10747 T13301 T13302 themeOf p65,binding
R10748 T13302 T13299 themeOf binding,effect
R10749 T13306 T13307 themeOf p65,binding
R10750 T13308 T13309 causeOf Tat,increased
R10751 T13310 T13309 themeOf binding,increased
R10752 T13310 T13317 themeOf binding,ineffective
R10753 T13311 T13310 themeOf p65,binding
R10754 T13312 T13317 causeOf "Tat C(22,25,27)A",ineffective
R10755 T13315 T13313 themeOf site,lacking
R10756 T13315 T13308 partOf site,Tat
R10757 T13315 T13314 themeOf site,binding
R10758 T13316 T13314 themeOf p65,binding
R10759 T13318 T13319 themeOf p65,binding
R10760 T13320 T13321 causeOf Tat,enhanced
R10761 T13322 T13323 themeOf p65,binding
R10762 T13323 T13321 themeOf binding,enhanced
R10710 T13242 T13246 themeOf Tat,association
R10711 T13244 T13245 themeOf p65,binding
R10712 T13245 T13243 themeOf binding,increases
R10713 T13246 T13243 causeOf association,increases
R10714 T13247 T13246 themeOf p65,association
R10715 T13248 T13249 themeOf Tat,interacted
R10716 T13250 T13249 themeOf p65,interacted
R10717 T13253 T13254 themeOf Tat,immunoprecipitated
R20919 T26470 T26471 themeOf Tat,associates
R20920 T26471 T26473 causeOf associates,increasing
R20921 T26472 T26471 themeOf p65,associates
R20922 T26472 T26474 themeOf p65,binding
R20923 T26474 T26473 themeOf binding,increasing

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T13723 0-3 P04610 denotes Tat
T13724 0-3 Q8UMQ1 denotes Tat
T13725 0-3 P04605 denotes Tat
T13726 0-3 P04608 denotes Tat
T13727 18-21 P21579 denotes p65
T13728 18-21 Q04206 denotes p65
T13729 71-74 Q04206 denotes p65
T13730 71-74 P21579 denotes p65
T13731 93-96 P04610 denotes Tat
T13732 93-96 Q8UMQ1 denotes Tat
T13733 93-96 P04608 denotes Tat
T13734 93-96 P04605 denotes Tat
T13735 124-127 P21579 denotes p65
T13736 124-127 Q04206 denotes p65
T13737 191-194 P21579 denotes p65
T13738 191-194 Q04206 denotes p65
T13739 204-207 P04608 denotes Tat
T13740 204-207 P04610 denotes Tat
T13741 204-207 P04605 denotes Tat
T13742 204-207 Q8UMQ1 denotes Tat
T13743 224-227 P04610 denotes Tat
T13744 224-227 P04608 denotes Tat
T13745 224-227 P04605 denotes Tat
T13746 224-227 Q8UMQ1 denotes Tat
T13747 275-278 P21579 denotes p65
T13748 275-278 Q04206 denotes p65
T13749 327-330 P04608 denotes Tat
T13750 327-330 Q8UMQ1 denotes Tat
T13751 327-330 P04610 denotes Tat
T13752 327-330 P04605 denotes Tat
T13753 346-349 P04610 denotes Tat
T13754 346-349 P04608 denotes Tat
T13755 346-349 P04605 denotes Tat
T13756 346-349 Q8UMQ1 denotes Tat
T13757 363-366 P04608 denotes Tat
T13758 363-366 P04605 denotes Tat
T13759 363-366 P04610 denotes Tat
T13760 363-366 Q8UMQ1 denotes Tat
T13761 381-384 P04608 denotes Tat
T13762 381-384 Q8UMQ1 denotes Tat
T13763 381-384 P04605 denotes Tat
T13764 381-384 P04610 denotes Tat
T13765 485-488 P04608 denotes Tat
T13766 485-488 P04605 denotes Tat
T13767 485-488 P04610 denotes Tat
T13768 485-488 Q8UMQ1 denotes Tat
T13769 604-607 P21579 denotes p65
T13770 604-607 Q04206 denotes p65
T13771 621-624 P04610 denotes Tat
T13772 621-624 Q8UMQ1 denotes Tat
T13773 621-624 P04605 denotes Tat
T13774 621-624 P04608 denotes Tat
T13775 641-644 P04605 denotes Tat
T13776 641-644 P04608 denotes Tat
T13777 641-644 Q8UMQ1 denotes Tat
T13778 641-644 P04610 denotes Tat
T13779 658-661 P04608 denotes Tat
T13780 658-661 P04610 denotes Tat
T13781 658-661 P04605 denotes Tat
T13782 658-661 Q8UMQ1 denotes Tat
T13783 676-679 Q8UMQ1 denotes Tat
T13784 676-679 P04610 denotes Tat
T13785 676-679 P04605 denotes Tat
T13786 676-679 P04608 denotes Tat
T13787 714-717 P04610 denotes Tat
T13788 714-717 Q8UMQ1 denotes Tat
T13789 714-717 P04608 denotes Tat
T13790 714-717 P04605 denotes Tat
T13791 773-776 P04610 denotes Tat
T13792 773-776 P04605 denotes Tat
T13793 773-776 Q8UMQ1 denotes Tat
T13794 773-776 P04608 denotes Tat
T13795 793-796 Q04206 denotes p65
T13796 793-796 P21579 denotes p65
T13797 2650-2653 P21579 denotes p65
T13798 2650-2653 Q04206 denotes p65
T13799 2685-2688 Q8UMQ1 denotes Tat
T13800 2685-2688 P04610 denotes Tat
T13801 2685-2688 P04608 denotes Tat
T13802 2685-2688 P04605 denotes Tat
T13803 2728-2731 P04608 denotes Tat
T13804 2728-2731 P04605 denotes Tat
T13805 2728-2731 P04610 denotes Tat
T13806 2728-2731 Q8UMQ1 denotes Tat
T13807 2749-2752 Q04206 denotes p65
T13808 2749-2752 P21579 denotes p65
T13809 2870-2873 P04610 denotes Tat
T13810 2870-2873 P04608 denotes Tat
T13811 2870-2873 Q8UMQ1 denotes Tat
T13812 2870-2873 P04605 denotes Tat
T13813 2984-2987 Q04206 denotes p65
T13814 2984-2987 P21579 denotes p65
T13815 3034-3037 P04605 denotes Tat
T13816 3034-3037 P04610 denotes Tat
T13817 3034-3037 Q8UMQ1 denotes Tat
T13818 3034-3037 P04608 denotes Tat
T13819 3064-3067 P04605 denotes Tat
T13820 3064-3067 P04608 denotes Tat
T13821 3064-3067 P04610 denotes Tat
T13822 3064-3067 Q8UMQ1 denotes Tat
T13823 3075-3078 Q04206 denotes p65
T13824 3075-3078 P21579 denotes p65
T13825 3122-3125 P21579 denotes p65
T13826 3122-3125 Q04206 denotes p65
T13827 3239-3242 P04610 denotes Tat
T13828 3239-3242 P04608 denotes Tat
T13829 3239-3242 P04605 denotes Tat
T13830 3239-3242 Q8UMQ1 denotes Tat
T13831 3246-3249 P04610 denotes Tat
T13832 3246-3249 Q8UMQ1 denotes Tat
T13833 3246-3249 P04608 denotes Tat
T13834 3246-3249 P04605 denotes Tat
T13835 3268-3271 Q04206 denotes p65
T13836 3268-3271 P21579 denotes p65
T13837 3324-3327 P04608 denotes Tat
T13838 3324-3327 P04610 denotes Tat
T13839 3324-3327 P04605 denotes Tat
T13840 3324-3327 Q8UMQ1 denotes Tat
T13841 3367-3370 Q04206 denotes p65
T13842 3367-3370 P21579 denotes p65
T13843 3390-3393 P04610 denotes Tat
T13844 3390-3393 P04608 denotes Tat
T13845 3390-3393 Q8UMQ1 denotes Tat
T13846 3390-3393 P04605 denotes Tat
T13847 3437-3440 Q04206 denotes p65
T13848 3437-3440 P21579 denotes p65
T13849 3475-3478 Q04206 denotes p65
T13850 3475-3478 P21579 denotes p65
T13851 3607-3610 P04608 denotes Tat
T13852 3607-3610 Q8UMQ1 denotes Tat
T13853 3607-3610 P04610 denotes Tat
T13854 3607-3610 P04605 denotes Tat
T13855 3638-3641 Q04206 denotes p65
T13856 3638-3641 P21579 denotes p65
T26505 920-923 P04610 denotes Tat
T26506 920-923 Q8UMQ1 denotes Tat
T26507 920-923 P04605 denotes Tat
T26508 920-923 P04608 denotes Tat
T26509 940-943 P21579 denotes p65
T26510 940-943 Q04206 denotes p65
T26511 1031-1034 P21579 denotes p65
T26512 1031-1034 Q04206 denotes p65
T26513 1076-1079 P04605 denotes Tat
T26514 1076-1079 P04608 denotes Tat
T26515 1076-1079 P04610 denotes Tat
T26516 1076-1079 Q8UMQ1 denotes Tat
T26517 1089-1092 P04610 denotes Tat
T26518 1089-1092 P04608 denotes Tat
T26519 1089-1092 P04605 denotes Tat
T26520 1089-1092 Q8UMQ1 denotes Tat
T26521 1114-1117 P04608 denotes Tat
T26522 1114-1117 P04605 denotes Tat
T26523 1114-1117 P04610 denotes Tat
T26524 1114-1117 Q8UMQ1 denotes Tat
T26525 1137-1140 Q8UMQ1 denotes Tat
T26526 1137-1140 P04610 denotes Tat
T26527 1137-1140 P04608 denotes Tat
T26528 1137-1140 P04605 denotes Tat
T26529 1163-1166 Q8UMQ1 denotes Tat
T26530 1163-1166 P04610 denotes Tat
T26531 1163-1166 P04608 denotes Tat
T26532 1163-1166 P04605 denotes Tat
T26533 1193-1196 Q8UMQ1 denotes Tat
T26534 1193-1196 P04610 denotes Tat
T26535 1193-1196 P04608 denotes Tat
T26536 1193-1196 P04605 denotes Tat
T26537 1397-1400 P21579 denotes p65
T26538 1397-1400 Q04206 denotes p65
T26539 1431-1434 P04608 denotes Tat
T26540 1431-1434 P04605 denotes Tat
T26541 1431-1434 P04610 denotes Tat
T26542 1431-1434 Q8UMQ1 denotes Tat
T26543 1440-1443 P04605 denotes Tat
T26544 1440-1443 P04610 denotes Tat
T26545 1440-1443 P04608 denotes Tat
T26546 1440-1443 Q8UMQ1 denotes Tat
T26547 1461-1464 P04610 denotes Tat
T26548 1461-1464 P04608 denotes Tat
T26549 1461-1464 P04605 denotes Tat
T26550 1461-1464 Q8UMQ1 denotes Tat
T26551 1480-1483 Q8UMQ1 denotes Tat
T26552 1480-1483 P04608 denotes Tat
T26553 1480-1483 P04610 denotes Tat
T26554 1480-1483 P04605 denotes Tat
T26555 1499-1502 P04610 denotes Tat
T26556 1499-1502 Q8UMQ1 denotes Tat
T26557 1499-1502 P04608 denotes Tat
T26558 1499-1502 P04605 denotes Tat
T26559 1691-1694 P21579 denotes p65
T26560 1691-1694 Q04206 denotes p65
T26561 1772-1775 Q04206 denotes p65
T26562 1772-1775 P21579 denotes p65
T26563 1831-1834 P04608 denotes Tat
T26564 1831-1834 P04610 denotes Tat
T26565 1831-1834 Q8UMQ1 denotes Tat
T26566 1831-1834 P04605 denotes Tat
T26567 1885-1888 P21579 denotes p65
T26568 1885-1888 Q04206 denotes p65
T26569 1982-1985 Q04206 denotes p65
T26570 1982-1985 P21579 denotes p65
T26571 2176-2179 P21579 denotes p65
T26572 2176-2179 Q04206 denotes p65
T26573 2356-2359 Q8UMQ1 denotes Tat
T26574 2356-2359 P04610 denotes Tat
T26575 2356-2359 P04608 denotes Tat
T26576 2356-2359 P04605 denotes Tat
T26577 2368-2371 Q8UMQ1 denotes Tat
T26578 2368-2371 P04610 denotes Tat
T26579 2368-2371 P04608 denotes Tat
T26580 2368-2371 P04605 denotes Tat