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PMC:3333881 / 23390-25483 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T25214 10-13 NN denotes Tat
T25215 14-22 VB denotes relieves
T25216 23-26 NN denotes p65
T25217 27-31 IN denotes from
T25218 32-35 DT denotes the
T25219 36-41 JJ denotes IκB-α
T25220 42-53 NN denotes inhibition.
T25221 54-55 -LRB- denotes (
T25222 55-56 NN denotes A
T25223 56-57 -RRB- denotes )
T25224 58-67 JJ denotes Schematic
T25225 68-82 NN denotes representation
T25226 83-85 IN denotes of
T25227 86-95 JJ denotes wild-type
T25228 96-99 CC denotes and
T25229 100-106 JJ denotes mutant
T25230 107-110 NN denotes Tat
T25231 111-120 NN denotes proteins.
T25232 121-122 -LRB- denotes (
T25233 122-123 NN denotes B
T25234 123-124 -RRB- denotes )
T25235 125-133 NN denotes HA-IκB-α
T25236 134-135 -LRB- denotes (
T25237 135-139 NN denotes 5 µl
T25238 139-140 -RRB- denotes )
T25239 141-144 VB denotes was
T25240 145-154 VB denotes incubated
T25241 155-157 IN denotes in
T25242 158-166 NN denotes presence
T25243 167-169 CC denotes or
T25244 170-177 NN denotes absence
T25245 178-180 IN denotes of
T25246 181-189 NN denotes FLAG-Tat
T25247 189-190 -COMMA- denotes ,
T25248 191-199 NN denotes FLAG-Tat
T25249 200-201 NNP denotes T
T25250 201-202 -COMMA- denotes ,
T25251 202-203 NN denotes N
T25252 203-204 -LRB- denotes (
T25253 204-209 CD denotes 23,24
T25254 209-210 -RRB- denotes )
T25255 210-211 NN denotes A
T25256 211-212 -COMMA- denotes ,
T25257 213-221 NN denotes FLAG-Tat
T25258 222-223 NN denotes K
T25259 223-224 -LRB- denotes (
T25260 224-229 CD denotes 50,51
T25261 229-230 -RRB- denotes )
T25262 230-231 NN denotes A
T25263 231-232 -COMMA- denotes ,
T25264 233-241 NN denotes FLAG-Tat
T25265 242-243 NN denotes R
T25266 243-244 -LRB- denotes (
T25267 244-249 CD denotes 49–57
T25268 249-250 -RRB- denotes )
T25269 250-251 NN denotes A
T25270 252-254 CC denotes or
T25271 255-263 NN denotes FLAG-Tat
T25272 264-265 NN denotes C
T25273 265-266 -LRB- denotes (
T25274 266-274 CD denotes 22,25,27
T25275 274-275 -RRB- denotes )
T25276 275-276 NN denotes A
T25277 277-278 -LRB- denotes (
T25278 278-283 NN denotes 10 µl
T25279 283-284 -RRB- denotes )
T25280 285-290 VB denotes using
T25281 291-293 FW denotes in
T25282 294-299 FW denotes vitro
T25283 300-310 VB denotes translated
T25284 311-319 NN denotes proteins
T25285 321-330 JJ denotes Wild-type
T25286 331-334 CC denotes and
T25287 335-341 JJ denotes mutant
T25288 342-345 NN denotes Tat
T25289 346-354 NN denotes proteins
T25290 355-359 VB denotes were
T25291 360-378 VB denotes immunoprecipitated
T25292 379-383 IN denotes with
T25293 384-393 JJ denotes anti-FLAG
T25294 394-402 NN denotes antibody
T25295 402-403 -COLON- denotes ;
T25296 404-419 NN denotes immunocomplexes
T25297 420-424 VB denotes were
T25298 425-434 VB denotes separated
T25299 435-437 IN denotes by
T25300 438-440 CD denotes 12
T25301 440-441 NN denotes %
T25302 442-450 NN denotes SDS–PAGE
T25303 451-454 CC denotes and
T25304 455-463 VB denotes analysed
T25305 464-466 IN denotes by
T25306 467-474 JJ denotes western
T25307 475-483 NN denotes blotting
T25308 484-488 IN denotes with
T25309 489-496 JJ denotes anti-HA
T25310 497-500 CC denotes and
T25311 501-510 JJ denotes anti-FLAG
T25312 511-522 NN denotes antibodies.
T25313 523-524 -LRB- denotes (
T25314 524-525 NN denotes C
T25315 525-526 -RRB- denotes )
T25316 527-549 JJ denotes 35S-methionine-labeled
T25317 550-553 NN denotes p65
T25318 554-555 -LRB- denotes (
T25319 555-559 NN denotes 5 µl
T25320 559-560 -RRB- denotes )
T25321 561-564 VB denotes was
T25322 565-574 VB denotes incubated
T25323 575-579 IN denotes with
T25324 580-582 FW denotes in
T25325 583-588 FW denotes vitro
T25326 589-599 VB denotes translated
T25327 600-608 NN denotes HA-IκB-α
T25328 609-610 -LRB- denotes (
T25329 610-614 NN denotes 5 µl
T25330 614-615 -RRB- denotes )
T25331 616-618 IN denotes in
T25332 619-627 NN denotes presence
T25333 628-630 CC denotes or
T25334 631-638 NN denotes absence
T25335 639-641 IN denotes of
T25336 642-650 NN denotes FLAG-Tat
T25337 651-653 CC denotes or
T25338 654-662 NN denotes FLAG-Tat
T25339 663-664 NN denotes R
T25340 664-665 -LRB- denotes (
T25341 665-670 CD denotes 49–57
T25342 670-671 -RRB- denotes )
T25343 671-672 NN denotes A
T25344 673-674 -LRB- denotes (
T25345 674-675 CD denotes 5
T25346 675-676 -COMMA- denotes ,
T25347 677-679 CD denotes 10
T25348 680-682 CC denotes or
T25349 683-688 NN denotes 20 µl
T25350 688-689 -RRB- denotes )
T25351 691-699 NN denotes HA-IκB-α
T25352 700-703 VB denotes was
T25353 704-722 VB denotes immunoprecipitated
T25354 723-727 IN denotes with
T25355 728-735 JJ denotes anti-HA
T25356 736-744 NN denotes antibody
T25357 744-745 -COLON- denotes ;
T25358 746-761 NN denotes immunocomplexes
T25359 762-766 VB denotes were
T25360 767-776 VB denotes separated
T25361 777-779 IN denotes by
T25362 780-782 CD denotes 12
T25363 782-783 NN denotes %
T25364 784-792 NN denotes SDS–PAGE
T25365 793-796 CC denotes and
T25366 797-805 VB denotes analysed
T25367 806-808 IN denotes by
T25368 809-816 JJ denotes western
T25369 817-825 NN denotes blotting
T25370 826-830 IN denotes with
T25371 831-838 JJ denotes anti-HA
T25372 839-842 CC denotes and
T25373 843-852 JJ denotes anti-FLAG
T25374 853-863 NN denotes antibodies
T25375 863-864 -COMMA- denotes ,
T25376 865-867 CC denotes or
T25377 868-883 NN denotes autoradiography
T25378 884-885 -LRB- denotes (
T25379 885-896 NN denotes 35S-Met-p65
T25380 896-897 -RRB- denotes )
T25381 899-911 NN denotes Densitometry
T25382 912-918 NN denotes values
T25383 919-920 -LRB- denotes (
T25384 920-921 NN denotes D
T25385 921-922 -RRB- denotes )
T25386 923-925 IN denotes of
T25387 926-929 DT denotes the
T25388 930-935 NN denotes bands
T25389 936-940 VB denotes were
T25390 941-950 VB denotes expressed
T25391 951-953 IN denotes as
T25392 954-958 JJ denotes fold
T25393 959-967 NN denotes increase
T25394 968-973 IN denotes above
T25395 974-977 DT denotes the
T25396 978-985 NN denotes control
T25397 986-987 -LRB- denotes (
T25398 987-991 NN denotes lane
T25399 992-993 CD denotes 1
T25400 993-994 -RRB- denotes )
T25401 994-995 -LRB- denotes .
T25402 996-997 NN denotes (
T25403 997-998 -RRB- denotes D
T25404 998-1001 DT denotes ) T
T25405 1001-1004 NN denotes he
T25406 1004-1007 NN denotes p65
T25407 1008-1015 NN denotes DNA bin
T25408 1015-1023 NN denotes ding act
T25409 1023-1026 VB denotes ivi
T25410 1026-1034 VB denotes ty was a
T25411 1034-1036 IN denotes na
T25412 1036-1040 NN denotes lyse
T25413 1040-1045 VB denotes d by
T25414 1045-1047 FW denotes EM
T25415 1047-1052 FW denotes SA us
T25416 1052-1062 VB denotes ing in vit
T25417 1062-1070 NN denotes ro trans
T25418 1086-1103 NN denotes 32P-labeled-NF-κB
T25419 1104-1119 JJ denotes double-stranded
T25420 1120-1135 NN denotes oligonucleotide
T25421 1136-1139 VB denotes was
T25422 1140-1149 VB denotes incubated
T25423 1150-1154 IN denotes with
T25424 1155-1158 NN denotes p65
T25425 1159-1160 -LRB- denotes (
T25426 1160-1166 NN denotes 0.5 µl
T25427 1166-1167 -RRB- denotes )
T25428 1168-1170 IN denotes in
T25429 1171-1179 NN denotes presence
T25430 1180-1182 CC denotes or
T25431 1183-1190 NN denotes absence
T25432 1191-1193 IN denotes of
T25433 1194-1202 NN denotes HA-IκB-α
T25434 1203-1204 -LRB- denotes (
T25435 1204-1208 NN denotes 1 µl
T25436 1208-1209 -RRB- denotes )
T25437 1209-1210 -COMMA- denotes ,
T25438 1211-1219 NN denotes FLAG-Tat
T25439 1220-1222 CC denotes or
T25440 1223-1231 NN denotes FLAG-Tat
T25441 1232-1233 NN denotes R
T25442 1233-1234 -LRB- denotes (
T25443 1234-1239 CD denotes 49–57
T25444 1239-1240 -RRB- denotes )
T25445 1240-1241 NN denotes A
T25446 1242-1243 -LRB- denotes (
T25447 1243-1244 CD denotes 5
T25448 1245-1248 CC denotes and
T25449 1249-1254 NN denotes 10 µl
T25450 1254-1255 -RRB- denotes )
T25451 1255-1256 -COLON- denotes ;
T25452 1257-1268 NN denotes competition
T25453 1269-1271 IN denotes of
T25454 1272-1275 NN denotes DNA
T25455 1276-1283 NN denotes binding
T25456 1284-1287 VB denotes was
T25457 1288-1297 VB denotes performed
T25458 1298-1302 IN denotes with
T25459 1303-1307 CD denotes 10 -
T25460 1308-1310 IN denotes up
T25461 1311-1313 TO denotes to
T25462 1314-1322 JJ denotes 100-fold
T25463 1323-1328 JJ denotes molar
T25464 1329-1335 NN denotes excess
T25465 1336-1338 IN denotes of
T25466 1339-1348 JJ denotes unlabeled
T25467 1349-1354 NN denotes NF-κB
T25468 1355-1370 JJ denotes double-stranded
T25469 1371-1386 NN denotes oligonucleotide
T25470 1388-1399 NN denotes DNA/protein
T25471 1400-1409 NN denotes complexes
T25472 1410-1414 VB denotes were
T25473 1415-1418 VB denotes run
T25474 1419-1421 IN denotes on
T25475 1422-1423 CD denotes 6
T25476 1423-1424 NN denotes %
T25477 1425-1433 NN denotes PAGE–TBE
T25478 1434-1437 CC denotes and
T25479 1438-1446 VB denotes analysed
T25480 1447-1449 IN denotes by
T25481 1450-1466 NN denotes autoradiography.
T25482 1467-1468 -LRB- denotes (
T25483 1468-1469 NN denotes E
T25484 1469-1470 -RRB- denotes )
T25485 1471-1487 NN denotes p50−/−p65−/−MEFs
T25486 1488-1489 -LRB- denotes (
T25487 1489-1496 CD denotes 3 × 105
T25488 1497-1502 NN denotes cells
T25489 1502-1503 -RRB- denotes )
T25490 1504-1508 VB denotes were
T25491 1509-1520 VB denotes transfected
T25492 1521-1525 IN denotes with
T25493 1526-1532 NN denotes pκBLuc
T25494 1533-1534 -LRB- denotes (
T25495 1534-1540 NN denotes 0.5 µg
T25496 1540-1541 -RRB- denotes )
T25497 1542-1545 CC denotes and
T25498 1546-1555 JJ denotes pSV-β-Gal
T25499 1556-1557 -LRB- denotes (
T25500 1557-1563 CD denotes 0.1 µg
T25501 1563-1564 -RRB- denotes )
T25502 1565-1569 IN denotes with
T25503 1570-1572 CC denotes or
T25504 1573-1580 IN denotes without
T25505 1581-1592 NN denotes pRc/CMV-p65
T25506 1593-1594 -LRB- denotes (
T25507 1594-1600 CD denotes 0.5 µg
T25508 1600-1601 -RRB- denotes )
T25509 1601-1602 -COMMA- denotes ,
T25510 1603-1617 NN denotes pCMV4-HA-IκB-α
T25511 1618-1619 -LRB- denotes (
T25512 1619-1625 CD denotes 0.5 µg
T25513 1625-1626 -RRB- denotes )
T25514 1626-1627 -COMMA- denotes ,
T25515 1628-1639 NN denotes p3xFLAG-Tat
T25516 1640-1642 CC denotes or
T25517 1643-1654 NN denotes p3xFLAG-Tat
T25518 1655-1656 NN denotes R
T25519 1656-1657 -LRB- denotes (
T25520 1657-1662 CD denotes 49–57
T25521 1662-1663 -RRB- denotes )
T25522 1663-1664 NN denotes A
T25523 1665-1666 -LRB- denotes (
T25524 1666-1669 CD denotes 0.5
T25525 1669-1670 -COMMA- denotes ,
T25526 1671-1672 CD denotes 1
T25527 1673-1676 CC denotes and
T25528 1677-1681 NN denotes 2 µg
T25529 1681-1682 -RRB- denotes )
T25530 1684-1687 DT denotes The
T25531 1688-1698 NN denotes luciferase
T25532 1699-1707 NN denotes activity
T25533 1708-1711 VB denotes was
T25534 1712-1720 VB denotes measured
T25535 1721-1723 IN denotes in
T25536 1724-1728 NN denotes cell
T25537 1729-1737 NN denotes extracts
T25538 1738-1742 JJ denotes 48-h
T25539 1743-1760 JJ denotes post-transfection
T25540 1761-1764 CC denotes and
T25541 1765-1775 VB denotes normalized
T25542 1776-1778 TO denotes to
T25543 1779-1794 JJ denotes β-galactosidase
T25544 1795-1803 NN denotes activity
T25545 1805-1809 JJ denotes Fold
T25546 1810-1820 NN denotes activation
T25547 1821-1824 VB denotes was
T25548 1825-1835 VB denotes calculated
T25549 1836-1844 JJ denotes relative
T25550 1845-1847 TO denotes to
T25551 1848-1860 NN denotes transfection
T25552 1861-1863 IN denotes of
T25553 1864-1867 DT denotes the
T25554 1868-1874 NN denotes pκBLuc
T25555 1875-1882 NN denotes plasmid
T25556 1883-1888 RB denotes alone
T25557 1890-1896 NN denotes Values
T25558 1897-1898 -LRB- denotes (
T25559 1898-1907 NN denotes mean ± SE
T25560 1907-1908 -COMMA- denotes ,
T25561 1909-1914 NN denotes n = 3
T25562 1914-1915 -RRB- denotes )
T25563 1916-1919 VB denotes are
T25564 1920-1925 VB denotes shown
T25565 1927-1929 IN denotes As
T25566 1930-1937 NN denotes control
T25567 1938-1940 IN denotes of
T25568 1941-1948 NN denotes protein
T25569 1949-1959 NN denotes expression
T25570 1959-1960 -COMMA- denotes ,
T25571 1961-1969 NN denotes aliquots
T25572 1970-1972 IN denotes of
T25573 1973-1977 NN denotes cell
T25574 1978-1986 NN denotes extracts
T25575 1987-1988 -LRB- denotes (
T25576 1988-1993 NN denotes 20 µg
T25577 1993-1994 -RRB- denotes )
T25578 1995-1999 VB denotes were
T25579 2000-2008 VB denotes analysed
T25580 2009-2011 IN denotes by
T25581 2012-2019 JJ denotes western
T25582 2020-2028 NN denotes blotting
T25583 2029-2033 IN denotes with
T25584 2034-2042 JJ denotes anti-p65
T25585 2042-2043 -COMMA- denotes ,
T25586 2044-2051 JJ denotes anti-HA
T25587 2051-2052 -COMMA- denotes ,
T25588 2053-2062 JJ denotes anti-FLAG
T25589 2063-2066 CC denotes and
T25590 2067-2081 JJ denotes anti-γ-tubulin
T25591 2082-2092 NN denotes antibodies
R20033 T25214 T25215 arg1Of Tat,relieves
R20034 T25214 T25280 arg1Of Tat,using
R20035 T25215 T25280 modOf relieves,using
R20036 T25216 T25217 arg1Of p65,from
R20037 T25216 T25226 arg1Of p65,of
R20038 T25216 T25263 arg1Of p65,","
R20039 T25222 T25221 arg2Of A,(
R20040 T25223 T25221 arg3Of ),(
R20041 T25225 T25217 arg2Of representation,from
R20042 T25225 T25218 arg1Of representation,the
R20043 T25225 T25219 arg1Of representation,IκB-α
R20044 T25225 T25220 arg1Of representation,inhibition.
R20045 T25225 T25221 arg1Of representation,(
R20046 T25225 T25224 arg1Of representation,Schematic
R20047 T25227 T25228 arg1Of wild-type,and
R20048 T25229 T25228 arg2Of mutant,and
R20049 T25231 T25227 arg1Of proteins.,wild-type
R20050 T25231 T25229 arg1Of proteins.,mutant
R20051 T25231 T25230 arg1Of proteins.,Tat
R20052 T25231 T25232 arg1Of proteins.,(
R20053 T25231 T25239 modOf proteins.,was
R20054 T25231 T25243 arg1Of proteins.,or
R20055 T25233 T25232 arg2Of B,(
R20056 T25234 T25232 arg3Of ),(
R20057 T25235 T25236 arg1Of HA-IκB-α,(
R20058 T25235 T25239 arg1Of HA-IκB-α,was
R20059 T25235 T25240 arg2Of HA-IκB-α,incubated
R20060 T25237 T25236 arg2Of 5 µl,(
R20061 T25238 T25236 arg3Of ),(
R20062 T25240 T25239 arg2Of incubated,was
R20063 T25240 T25241 arg1Of incubated,in
R20064 T25242 T25241 arg2Of presence,in
R20065 T25243 T25226 arg2Of or,of
R20066 T25244 T25245 arg1Of absence,of
R20067 T25244 T25256 arg1Of absence,","
R20068 T25246 T25245 arg2Of FLAG-Tat,of
R20069 T25246 T25247 arg1Of FLAG-Tat,","
R20070 T25246 T25250 arg1Of FLAG-Tat,","
R20071 T25249 T25247 arg2Of T,","
R20072 T25249 T25248 arg1Of T,FLAG-Tat
R20073 T25253 T25252 arg2Of "23,24",(
R20074 T25254 T25252 arg3Of ),(
R20075 T25255 T25250 arg2Of A,","
R20076 T25255 T25251 arg1Of A,N
R20077 T25255 T25252 arg1Of A,(
R20078 T25256 T25243 arg2Of ",",or
R20079 T25260 T25259 arg2Of "50,51",(
R20080 T25261 T25259 arg3Of ),(
R20081 T25262 T25256 arg2Of A,","
R20082 T25262 T25257 arg1Of A,FLAG-Tat
R20083 T25262 T25258 arg1Of A,K
R20084 T25262 T25259 arg1Of A,(
R20085 T25263 T25270 arg1Of ",",or
R20086 T25265 T25264 arg1Of R,FLAG-Tat
R20087 T25265 T25266 arg1Of R,(
R20088 T25267 T25266 arg2Of 49–57,(
R20089 T25268 T25266 arg3Of ),(
R20090 T25269 T25263 arg2Of A,","
R20091 T25269 T25265 arg1Of A,R
R20092 T25270 T25215 arg2Of or,relieves
R20093 T25274 T25273 arg2Of "22,25,27",(
R20094 T25275 T25273 arg3Of ),(
R20095 T25276 T25270 arg2Of A,or
R20096 T25276 T25271 arg1Of A,FLAG-Tat
R20097 T25276 T25272 arg1Of A,C
R20098 T25276 T25273 arg1Of A,(
R20099 T25276 T25277 arg1Of A,(
R20100 T25278 T25277 arg2Of 10 µl,(
R20101 T25279 T25277 arg3Of ),(
R20102 T25284 T25280 arg2Of proteins,using
R20103 T25284 T25281 arg1Of proteins,in
R20104 T25284 T25282 arg1Of proteins,vitro
R20105 T25284 T25283 arg2Of proteins,translated
R20106 T25285 T25286 arg1Of Wild-type,and
R20107 T25287 T25286 arg2Of mutant,and
R20108 T25289 T25285 arg1Of proteins,Wild-type
R20109 T25289 T25287 arg1Of proteins,mutant
R20110 T25289 T25288 arg1Of proteins,Tat
R20111 T25289 T25290 arg1Of proteins,were
R20112 T25289 T25291 arg2Of proteins,immunoprecipitated
R20113 T25291 T25290 arg2Of immunoprecipitated,were
R20114 T25291 T25292 arg1Of immunoprecipitated,with
R20115 T25291 T25295 arg1Of immunoprecipitated,;
R20116 T25294 T25292 arg2Of antibody,with
R20117 T25294 T25293 arg1Of antibody,anti-FLAG
R20118 T25296 T25297 arg1Of immunocomplexes,were
R20119 T25296 T25298 arg2Of immunocomplexes,separated
R20120 T25296 T25304 arg2Of immunocomplexes,analysed
R20121 T25298 T25303 arg1Of separated,and
R20122 T25300 T25301 arg1Of 12,%
R20123 T25302 T25298 arg1Of SDS–PAGE,separated
R20124 T25302 T25299 arg2Of SDS–PAGE,by
R20125 T25302 T25300 arg1Of SDS–PAGE,12
R20126 T25303 T25295 arg2Of and,;
R20127 T25303 T25297 arg2Of and,were
R20128 T25303 T25308 arg1Of and,with
R20129 T25304 T25303 arg2Of analysed,and
R20130 T25307 T25304 arg1Of blotting,analysed
R20131 T25307 T25305 arg2Of blotting,by
R20132 T25307 T25306 arg1Of blotting,western
R20133 T25309 T25310 arg1Of anti-HA,and
R20134 T25311 T25310 arg2Of anti-FLAG,and
R20135 T25312 T25308 arg2Of antibodies.,with
R20136 T25312 T25309 arg1Of antibodies.,anti-HA
R20137 T25312 T25311 arg1Of antibodies.,anti-FLAG
R20138 T25312 T25313 arg1Of antibodies.,(
R20139 T25312 T25321 modOf antibodies.,was
R20140 T25314 T25313 arg2Of C,(
R20141 T25315 T25313 arg3Of ),(
R20142 T25317 T25316 arg1Of p65,35S-methionine-labeled
R20143 T25317 T25318 arg1Of p65,(
R20144 T25317 T25321 arg1Of p65,was
R20145 T25317 T25322 arg2Of p65,incubated
R20146 T25319 T25318 arg2Of 5 µl,(
R20147 T25320 T25318 arg3Of ),(
R20148 T25322 T25321 arg2Of incubated,was
R20149 T25322 T25323 arg1Of incubated,with
R20150 T25325 T25324 arg1Of vitro,in
R20151 T25327 T25323 arg2Of HA-IκB-α,with
R20152 T25327 T25325 arg1Of HA-IκB-α,vitro
R20153 T25327 T25326 arg2Of HA-IκB-α,translated
R20154 T25327 T25328 arg1Of HA-IκB-α,(
R20155 T25327 T25331 arg1Of HA-IκB-α,in
R20156 T25327 T25335 arg1Of HA-IκB-α,of
R20157 T25329 T25328 arg2Of 5 µl,(
R20158 T25330 T25328 arg3Of ),(
R20159 T25332 T25333 arg1Of presence,or
R20160 T25333 T25331 arg2Of or,in
R20161 T25334 T25333 arg2Of absence,or
R20162 T25336 T25337 arg1Of FLAG-Tat,or
R20163 T25339 T25337 arg2Of R,or
R20164 T25339 T25338 arg1Of R,FLAG-Tat
R20165 T25339 T25340 arg1Of R,(
R20166 T25341 T25340 arg2Of 49–57,(
R20167 T25342 T25340 arg3Of ),(
R20168 T25343 T25335 arg2Of A,of
R20169 T25343 T25336 arg1Of A,FLAG-Tat
R20170 T25343 T25339 arg1Of A,R
R20171 T25343 T25344 arg1Of A,(
R20172 T25345 T25346 arg1Of 5,","
R20173 T25346 T25348 arg1Of ",",or
R20174 T25347 T25346 arg2Of 10,","
R20175 T25348 T25344 arg2Of or,(
R20176 T25349 T25348 arg2Of 20 µl,or
R20177 T25350 T25344 arg3Of ),(
R20178 T25351 T25352 arg1Of HA-IκB-α,was
R20179 T25351 T25353 arg2Of HA-IκB-α,immunoprecipitated
R20180 T25353 T25352 arg2Of immunoprecipitated,was
R20181 T25353 T25354 arg1Of immunoprecipitated,with
R20182 T25353 T25357 arg1Of immunoprecipitated,;
R20183 T25356 T25354 arg2Of antibody,with
R20184 T25356 T25355 arg1Of antibody,anti-HA
R20185 T25358 T25359 arg1Of immunocomplexes,were
R20186 T25358 T25360 arg2Of immunocomplexes,separated
R20187 T25358 T25366 arg2Of immunocomplexes,analysed
R20188 T25360 T25365 arg1Of separated,and
R20189 T25362 T25363 arg1Of 12,%
R20190 T25364 T25360 arg1Of SDS–PAGE,separated
R20191 T25364 T25361 arg2Of SDS–PAGE,by
R20192 T25364 T25362 arg1Of SDS–PAGE,12
R20193 T25365 T25357 arg2Of and,;
R20194 T25365 T25359 arg2Of and,were
R20195 T25366 T25365 arg2Of analysed,and
R20196 T25369 T25368 arg1Of blotting,western
R20197 T25369 T25370 arg1Of blotting,with
R20198 T25369 T25376 arg1Of blotting,or
R20199 T25371 T25372 arg1Of anti-HA,and
R20200 T25373 T25372 arg2Of anti-FLAG,and
R20201 T25374 T25370 arg2Of antibodies,with
R20202 T25374 T25371 arg1Of antibodies,anti-HA
R20203 T25374 T25373 arg1Of antibodies,anti-FLAG
R20204 T25376 T25366 arg1Of or,analysed
R20205 T25376 T25367 arg2Of or,by
R20206 T25376 T25375 arg1Of or,","
R20207 T25377 T25376 arg2Of autoradiography,or
R20208 T25377 T25378 arg1Of autoradiography,(
R20209 T25379 T25378 arg2Of 35S-Met-p65,(
R20210 T25380 T25378 arg3Of ),(
R20211 T25382 T25381 arg1Of values,Densitometry
R20212 T25382 T25383 arg1Of values,(
R20213 T25382 T25386 arg1Of values,of
R20214 T25382 T25389 arg1Of values,were
R20215 T25382 T25390 arg2Of values,expressed
R20216 T25384 T25383 arg2Of D,(
R20217 T25385 T25383 arg3Of ),(
R20218 T25388 T25386 arg2Of bands,of
R20219 T25388 T25387 arg1Of bands,the
R20220 T25390 T25389 arg2Of expressed,were
R20221 T25390 T25391 arg1Of expressed,as
R20222 T25393 T25391 arg2Of increase,as
R20223 T25393 T25392 arg1Of increase,fold
R20224 T25393 T25394 arg1Of increase,above
R20225 T25393 T25401 arg1Of increase,.
R20226 T25396 T25394 arg2Of control,above
R20227 T25396 T25395 arg1Of control,the
R20228 T25396 T25397 arg1Of control,(
R20229 T25398 T25397 arg2Of lane,(
R20230 T25398 T25399 arg1Of lane,1
R20231 T25400 T25397 arg3Of ),(
R20232 T25402 T25401 arg2Of (,.
R20233 T25403 T25401 arg3Of D,.
R20234 T25408 T25404 arg1Of ding act,) T
R20235 T25408 T25405 arg1Of ding act,he
R20236 T25408 T25406 arg1Of ding act,p65
R20237 T25408 T25407 arg1Of ding act,DNA bin
R20238 T25408 T25409 arg1Of ding act,ivi
R20239 T25408 T25410 arg2Of ding act,ty was a
R20240 T25410 T25389 modOf ty was a,were
R20241 T25410 T25409 arg2Of ty was a,ivi
R20242 T25412 T25410 arg1Of lyse,ty was a
R20243 T25412 T25411 arg2Of lyse,na
R20244 T25412 T25413 arg1Of lyse,d by
R20245 T25415 T25414 arg1Of SA us,EM
R20246 T25417 T25413 arg2Of ro trans,d by
R20247 T25417 T25415 arg1Of ro trans,SA us
R20248 T25417 T25416 arg2Of ro trans,ing in vit
R20249 T25420 T25418 arg1Of oligonucleotide,32P-labeled-NF-κB
R20250 T25420 T25419 arg1Of oligonucleotide,double-stranded
R20251 T25420 T25421 arg1Of oligonucleotide,was
R20252 T25420 T25422 arg2Of oligonucleotide,incubated
R20253 T25422 T25421 arg2Of incubated,was
R20254 T25422 T25423 arg1Of incubated,with
R20255 T25422 T25451 arg1Of incubated,;
R20256 T25424 T25423 arg2Of p65,with
R20257 T25424 T25425 arg1Of p65,(
R20258 T25424 T25428 arg1Of p65,in
R20259 T25424 T25432 arg1Of p65,of
R20260 T25426 T25425 arg2Of 0.5 µl,(
R20261 T25427 T25425 arg3Of ),(
R20262 T25429 T25430 arg1Of presence,or
R20263 T25430 T25428 arg2Of or,in
R20264 T25431 T25430 arg2Of absence,or
R20265 T25433 T25434 arg1Of HA-IκB-α,(
R20266 T25433 T25437 arg1Of HA-IκB-α,","
R20267 T25435 T25434 arg2Of 1 µl,(
R20268 T25436 T25434 arg3Of ),(
R20269 T25437 T25439 arg1Of ",",or
R20270 T25438 T25437 arg2Of FLAG-Tat,","
R20271 T25439 T25432 arg2Of or,of
R20272 T25441 T25440 arg1Of R,FLAG-Tat
R20273 T25441 T25442 arg1Of R,(
R20274 T25443 T25442 arg2Of 49–57,(
R20275 T25444 T25442 arg3Of ),(
R20276 T25445 T25439 arg2Of A,or
R20277 T25445 T25441 arg1Of A,R
R20278 T25445 T25446 arg1Of A,(
R20279 T25447 T25448 arg1Of 5,and
R20280 T25448 T25446 arg2Of and,(
R20281 T25449 T25448 arg2Of 10 µl,and
R20282 T25450 T25446 arg3Of ),(
R20283 T25452 T25453 arg1Of competition,of
R20284 T25452 T25456 arg1Of competition,was
R20285 T25452 T25457 arg2Of competition,performed
R20286 T25455 T25453 arg2Of binding,of
R20287 T25455 T25454 arg1Of binding,DNA
R20288 T25457 T25451 arg2Of performed,;
R20289 T25457 T25456 arg2Of performed,was
R20290 T25457 T25458 arg1Of performed,with
R20291 T25461 T25460 arg1Of to,up
R20292 T25464 T25458 arg2Of excess,with
R20293 T25464 T25459 arg1Of excess,10 -
R20294 T25464 T25461 arg1Of excess,to
R20295 T25464 T25462 arg1Of excess,100-fold
R20296 T25464 T25463 arg1Of excess,molar
R20297 T25464 T25465 arg1Of excess,of
R20298 T25469 T25465 arg2Of oligonucleotide,of
R20299 T25469 T25466 arg1Of oligonucleotide,unlabeled
R20300 T25469 T25467 arg1Of oligonucleotide,NF-κB
R20301 T25469 T25468 arg1Of oligonucleotide,double-stranded
R20302 T25471 T25470 arg1Of complexes,DNA/protein
R20303 T25471 T25472 arg1Of complexes,were
R20304 T25471 T25473 arg2Of complexes,run
R20305 T25471 T25479 arg2Of complexes,analysed
R20306 T25473 T25474 arg1Of run,on
R20307 T25473 T25478 arg1Of run,and
R20308 T25475 T25476 arg1Of 6,%
R20309 T25477 T25474 arg2Of PAGE–TBE,on
R20310 T25477 T25475 arg1Of PAGE–TBE,6
R20311 T25478 T25472 arg2Of and,were
R20312 T25479 T25478 arg2Of analysed,and
R20313 T25481 T25479 arg1Of autoradiography.,analysed
R20314 T25481 T25480 arg2Of autoradiography.,by
R20315 T25481 T25482 arg1Of autoradiography.,(
R20316 T25481 T25490 modOf autoradiography.,were
R20317 T25483 T25482 arg2Of E,(
R20318 T25484 T25482 arg3Of ),(
R20319 T25485 T25486 arg1Of p50−/−p65−/−MEFs,(
R20320 T25485 T25490 arg1Of p50−/−p65−/−MEFs,were
R20321 T25485 T25491 arg2Of p50−/−p65−/−MEFs,transfected
R20322 T25488 T25486 arg2Of cells,(
R20323 T25488 T25487 arg1Of cells,3 × 105
R20324 T25489 T25486 arg3Of ),(
R20325 T25491 T25490 arg2Of transfected,were
R20326 T25491 T25492 arg1Of transfected,with
R20327 T25493 T25494 arg1Of pκBLuc,(
R20328 T25493 T25497 arg1Of pκBLuc,and
R20329 T25495 T25494 arg2Of 0.5 µg,(
R20330 T25496 T25494 arg3Of ),(
R20331 T25497 T25492 arg2Of and,with
R20332 T25498 T25499 arg1Of pSV-β-Gal,(
R20333 T25498 T25502 arg1Of pSV-β-Gal,with
R20334 T25498 T25504 arg1Of pSV-β-Gal,without
R20335 T25500 T25499 arg2Of 0.1 µg,(
R20336 T25501 T25499 arg3Of ),(
R20337 T25502 T25503 arg1Of with,or
R20338 T25504 T25503 arg2Of without,or
R20339 T25505 T25506 arg1Of pRc/CMV-p65,(
R20340 T25505 T25509 arg1Of pRc/CMV-p65,","
R20341 T25507 T25506 arg2Of 0.5 µg,(
R20342 T25508 T25506 arg3Of ),(
R20343 T25509 T25514 arg1Of ",",","
R20344 T25510 T25509 arg2Of pCMV4-HA-IκB-α,","
R20345 T25510 T25511 arg1Of pCMV4-HA-IκB-α,(
R20346 T25512 T25511 arg2Of 0.5 µg,(
R20347 T25513 T25511 arg3Of ),(
R20348 T25514 T25516 arg1Of ",",or
R20349 T25515 T25514 arg2Of p3xFLAG-Tat,","
R20350 T25516 T25502 arg2Of or,with
R20351 T25516 T25504 arg2Of or,without
R20352 T25518 T25516 arg2Of R,or
R20353 T25518 T25517 arg1Of R,p3xFLAG-Tat
R20354 T25518 T25519 arg1Of R,(
R20355 T25520 T25519 arg2Of 49–57,(
R20356 T25521 T25519 arg3Of ),(
R20357 T25522 T25497 arg2Of A,and
R20358 T25522 T25498 arg1Of A,pSV-β-Gal
R20359 T25522 T25523 arg1Of A,(
R20360 T25524 T25525 arg1Of 0.5,","
R20361 T25525 T25527 arg1Of ",",and
R20362 T25526 T25525 arg2Of 1,","
R20363 T25527 T25523 arg2Of and,(
R20364 T25528 T25527 arg2Of 2 µg,and
R20365 T25529 T25523 arg3Of ),(
R20366 T25532 T25530 arg1Of activity,The
R20367 T25532 T25531 arg1Of activity,luciferase
R20368 T25532 T25533 arg1Of activity,was
R20369 T25532 T25534 arg2Of activity,measured
R20370 T25532 T25541 arg2Of activity,normalized
R20371 T25534 T25535 arg1Of measured,in
R20372 T25534 T25540 arg1Of measured,and
R20373 T25537 T25535 arg2Of extracts,in
R20374 T25537 T25536 arg1Of extracts,cell
R20375 T25537 T25539 arg1Of extracts,post-transfection
R20376 T25539 T25538 arg1Of post-transfection,48-h
R20377 T25540 T25533 arg2Of and,was
R20378 T25541 T25540 arg2Of normalized,and
R20379 T25541 T25542 arg1Of normalized,to
R20380 T25544 T25542 arg2Of activity,to
R20381 T25544 T25543 arg1Of activity,β-galactosidase
R20382 T25546 T25545 arg1Of activation,Fold
R20383 T25546 T25547 arg1Of activation,was
R20384 T25546 T25548 arg2Of activation,calculated
R20385 T25548 T25547 arg2Of calculated,was
R20386 T25548 T25550 arg1Of calculated,to
R20387 T25550 T25549 arg1Of to,relative
R20388 T25551 T25550 arg2Of transfection,to
R20389 T25551 T25552 arg1Of transfection,of
R20390 T25555 T25552 arg2Of plasmid,of
R20391 T25555 T25553 arg1Of plasmid,the
R20392 T25555 T25554 arg1Of plasmid,pκBLuc
R20393 T25555 T25556 arg1Of plasmid,alone
R20394 T25557 T25558 arg1Of Values,(
R20395 T25557 T25563 arg1Of Values,are
R20396 T25557 T25564 arg2Of Values,shown
R20397 T25559 T25558 arg2Of mean ± SE,(
R20398 T25559 T25560 arg1Of mean ± SE,","
R20399 T25561 T25560 arg2Of n = 3,","
R20400 T25562 T25558 arg3Of ),(
R20401 T25564 T25563 arg2Of shown,are
R20402 T25566 T25565 arg2Of control,As
R20403 T25566 T25567 arg1Of control,of
R20404 T25569 T25567 arg2Of expression,of
R20405 T25569 T25568 arg1Of expression,protein
R20406 T25571 T25572 arg1Of aliquots,of
R20407 T25571 T25578 arg1Of aliquots,were
R20408 T25571 T25579 arg2Of aliquots,analysed
R20409 T25574 T25572 arg2Of extracts,of
R20410 T25574 T25573 arg1Of extracts,cell
R20411 T25574 T25575 arg1Of extracts,(
R20412 T25576 T25575 arg2Of 20 µg,(
R20413 T25577 T25575 arg3Of ),(
R20414 T25579 T25565 arg1Of analysed,As
R20415 T25579 T25570 arg1Of analysed,","
R20416 T25579 T25578 arg2Of analysed,were
R20417 T25582 T25579 arg1Of blotting,analysed
R20418 T25582 T25580 arg2Of blotting,by
R20419 T25582 T25581 arg1Of blotting,western
R20420 T25582 T25583 arg1Of blotting,with
R20421 T25584 T25585 arg1Of anti-p65,","
R20422 T25585 T25587 arg1Of ",",","
R20423 T25586 T25585 arg2Of anti-HA,","
R20424 T25587 T25589 arg1Of ",",and
R20425 T25588 T25587 arg2Of anti-FLAG,","
R20426 T25590 T25589 arg2Of anti-γ-tubulin,and
R20427 T25591 T25583 arg2Of antibodies,with
R20428 T25591 T25584 arg1Of antibodies,anti-p65
R20429 T25591 T25586 arg1Of antibodies,anti-HA
R20430 T25591 T25588 arg1Of antibodies,anti-FLAG
R20431 T25591 T25590 arg1Of antibodies,anti-γ-tubulin

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T25677 10-13 NNP denotes Tat
T25678 14-22 VBZ denotes relieves
T25679 23-26 CD denotes p65
T25680 27-31 IN denotes from
T25681 32-35 DT denotes the
T25682 36-41 JJ denotes IκB-α
T25683 42-52 NN denotes inhibition
T25684 52-53 . denotes .
T25685 54-55 -LRB- denotes (
T25686 55-56 NN denotes A
T25687 56-57 -RRB- denotes )
T25688 58-67 JJ denotes Schematic
T25689 68-82 NN denotes representation
T25690 83-85 IN denotes of
T25691 86-95 JJ denotes wild-type
T25692 96-99 CC denotes and
T25693 100-106 JJ denotes mutant
T25694 107-110 NNP denotes Tat
T25695 111-119 NNS denotes proteins
T25696 119-120 . denotes .
T25697 121-122 -LRB- denotes (
T25698 122-123 NNP denotes B
T25699 123-124 -RRB- denotes )
T25700 125-133 NNP denotes HA-IκB-α
T25701 134-135 -LRB- denotes (
T25702 135-136 CD denotes 5
T25703 137-139 NN denotes µl
T25704 139-140 -RRB- denotes )
T25705 141-144 VBD denotes was
T25706 145-154 VBN denotes incubated
T25707 155-157 IN denotes in
T25708 158-166 NN denotes presence
T25709 167-169 CC denotes or
T25710 170-177 NN denotes absence
T25711 178-180 IN denotes of
T25712 181-189 NNP denotes FLAG-Tat
T25713 189-190 , denotes ,
T25714 191-199 NNP denotes FLAG-Tat
T25715 200-201 NNP denotes T
T25716 201-202 , denotes ,
T25717 402-403 : denotes ;
T25718 202-203 NNP denotes N
T25719 203-204 -LRB- denotes (
T25720 204-209 CD denotes 23,24
T25721 209-210 -RRB- denotes )
T25722 210-211 DT denotes A
T25723 211-212 , denotes ,
T25724 213-221 JJ denotes FLAG-Tat
T25725 222-223 NNP denotes K
T25726 223-224 -LRB- denotes (
T25727 224-229 CD denotes 50,51
T25728 229-230 -RRB- denotes )
T25729 230-231 DT denotes A
T25730 231-232 , denotes ,
T25731 233-241 JJ denotes FLAG-Tat
T25732 242-243 NN denotes R
T25733 243-244 -LRB- denotes (
T25734 244-246 CD denotes 49
T25735 247-249 CD denotes 57
T25736 249-250 -RRB- denotes )
T25737 250-251 DT denotes A
T25738 252-254 CC denotes or
T25739 255-263 JJ denotes FLAG-Tat
T25740 264-265 NNP denotes C
T25741 265-266 -LRB- denotes (
T25742 266-274 CD denotes 22,25,27
T25743 274-275 -RRB- denotes )
T25744 275-276 DT denotes A
T25745 277-278 -LRB- denotes (
T25746 278-280 CD denotes 10
T25747 281-283 NN denotes µl
T25748 283-284 -RRB- denotes )
T25749 285-290 VBG denotes using
T25750 291-293 IN denotes in
T25751 294-299 NN denotes vitro
T25752 300-310 VBN denotes translated
T25753 311-319 NNS denotes proteins
T25754 319-320 . denotes .
T25755 321-330 JJ denotes Wild-type
T25756 331-334 CC denotes and
T25757 335-341 JJ denotes mutant
T25758 342-345 NNP denotes Tat
T25759 346-354 NNS denotes proteins
T25760 355-359 VBD denotes were
T25761 360-378 VBN denotes immunoprecipitated
T25762 379-383 IN denotes with
T25763 384-393 JJ denotes anti-FLAG
T25764 394-402 NN denotes antibody
T25765 404-419 NNS denotes immunocomplexes
T25766 420-424 VBD denotes were
T25767 425-434 VBN denotes separated
T25768 435-437 IN denotes by
T25769 438-440 CD denotes 12
T25770 440-441 NN denotes %
T25771 442-445 NNP denotes SDS
T25772 446-450 NNP denotes PAGE
T25773 451-454 CC denotes and
T25774 455-463 VBN denotes analysed
T25775 464-466 IN denotes by
T25776 467-474 JJ denotes western
T25777 475-483 VBG denotes blotting
T25778 484-488 IN denotes with
T25779 489-496 JJ denotes anti-HA
T25780 497-500 CC denotes and
T25781 501-510 JJ denotes anti-FLAG
T25782 511-521 NNS denotes antibodies
T25783 521-522 . denotes .
T25784 523-524 -LRB- denotes (
T25785 524-525 $ denotes C
T25786 525-526 -RRB- denotes )
T25787 527-549 JJ denotes 35S-methionine-labeled
T25788 550-553 NNS denotes p65
T25789 554-555 -LRB- denotes (
T25790 555-556 CD denotes 5
T25791 557-559 NN denotes µl
T25792 559-560 -RRB- denotes )
T25793 561-564 VBD denotes was
T25794 565-574 VBN denotes incubated
T25795 575-579 IN denotes with
T25796 580-582 IN denotes in
T25797 583-588 NN denotes vitro
T25798 589-599 VBN denotes translated
T25799 600-608 NNP denotes HA-IκB-α
T25800 609-610 -LRB- denotes (
T25801 610-611 CD denotes 5
T25802 612-614 NN denotes µl
T25803 614-615 -RRB- denotes )
T25804 616-618 IN denotes in
T25805 619-627 NN denotes presence
T25806 628-630 CC denotes or
T25807 631-638 NN denotes absence
T25808 639-641 IN denotes of
T25809 642-650 NN denotes FLAG-Tat
T25810 651-653 CC denotes or
T25811 654-662 NN denotes FLAG-Tat
T25812 663-664 NN denotes R
T25813 664-665 -LRB- denotes (
T25814 665-667 CD denotes 49
T25815 668-670 CD denotes 57
T25816 670-671 -RRB- denotes )
T25817 671-672 DT denotes A
T25818 673-674 -LRB- denotes (
T25819 674-675 CD denotes 5
T25820 675-676 , denotes ,
T25821 677-679 CD denotes 10
T25822 680-682 CC denotes or
T25823 683-685 CD denotes 20
T25824 686-688 NN denotes µl
T25825 688-689 -RRB- denotes )
T25826 689-690 . denotes .
T25827 691-699 NNP denotes HA-IκB-α
T25828 700-703 VBD denotes was
T25829 704-722 VBN denotes immunoprecipitated
T25830 723-727 IN denotes with
T25831 728-735 JJ denotes anti-HA
T25832 736-744 NN denotes antibody
T25833 744-745 : denotes ;
T25834 746-761 NNS denotes immunocomplexes
T25835 762-766 VBD denotes were
T25836 767-776 VBN denotes separated
T25837 777-779 IN denotes by
T25838 780-782 CD denotes 12
T25839 782-783 NN denotes %
T25840 784-787 NNP denotes SDS
T25841 788-792 NNP denotes PAGE
T25842 793-796 CC denotes and
T25843 797-805 VBN denotes analysed
T25844 806-808 IN denotes by
T25845 809-816 JJ denotes western
T25846 817-825 VBG denotes blotting
T25847 826-830 IN denotes with
T25848 831-838 JJ denotes anti-HA
T25849 839-842 CC denotes and
T25850 843-852 JJ denotes anti-FLAG
T25851 853-863 NNS denotes antibodies
T25852 863-864 , denotes ,
T25853 865-867 CC denotes or
T25854 868-883 NN denotes autoradiography
T25855 884-885 -LRB- denotes (
T25856 885-896 NNP denotes 35S-Met-p65
T25857 896-897 -RRB- denotes )
T25858 897-898 . denotes .
T25859 899-911 NNP denotes Densitometry
T25860 912-918 NNS denotes values
T25861 919-920 -LRB- denotes (
T25862 920-921 NNP denotes D
T25863 921-922 -RRB- denotes )
T25864 923-925 IN denotes of
T25865 926-929 DT denotes the
T25866 930-935 NNS denotes bands
T25867 936-940 VBD denotes were
T25868 941-950 VBN denotes expressed
T25869 951-953 IN denotes as
T25870 954-958 VBP denotes fold
T25871 959-967 NN denotes increase
T25872 968-973 IN denotes above
T25873 974-977 DT denotes the
T25874 978-985 NN denotes control
T25875 986-987 -LRB- denotes (
T25876 987-991 NN denotes lane
T25877 992-993 CD denotes 1
T25878 993-994 -RRB- denotes )
T25879 994-995 . denotes .
T25880 996-997 -LRB- denotes (
T25881 997-998 NNP denotes D
T25882 998-999 -RRB- denotes )
T25883 1000-1003 DT denotes The
T25884 1004-1007 CD denotes p65
T25885 1008-1011 NNP denotes DNA
T25886 1012-1019 JJ denotes binding
T25887 1020-1028 NN denotes activity
T25888 1029-1032 VBD denotes was
T25889 1033-1041 VBN denotes analysed
T25890 1042-1044 IN denotes by
T25891 1045-1049 NNP denotes EMSA
T25892 1050-1055 VBG denotes using
T25893 1056-1058 IN denotes in
T25894 1059-1064 NN denotes vitro
T25895 1065-1075 VBN denotes translated
T25896 1076-1084 NNS denotes proteins
T25897 1084-1085 . denotes .
T25898 1086-1103 JJ denotes 32P-labeled-NF-κB
T25899 1104-1119 JJ denotes double-stranded
T25900 1120-1135 NN denotes oligonucleotide
T25901 1136-1139 VBD denotes was
T25902 1140-1149 VBN denotes incubated
T25903 1150-1154 IN denotes with
T25904 1155-1158 NNS denotes p65
T25905 1159-1160 -LRB- denotes (
T25906 1160-1163 CD denotes 0.5
T25907 1164-1166 NN denotes µl
T25908 1166-1167 -RRB- denotes )
T25909 1168-1170 IN denotes in
T25910 1171-1179 NN denotes presence
T25911 1180-1182 CC denotes or
T25912 1183-1190 NN denotes absence
T25913 1191-1193 IN denotes of
T25914 1194-1202 NNP denotes HA-IκB-α
T25915 1203-1204 -LRB- denotes (
T25916 1204-1205 CD denotes 1
T25917 1206-1208 NN denotes µl
T25918 1208-1209 -RRB- denotes )
T25919 1209-1210 , denotes ,
T25920 1211-1219 JJ denotes FLAG-Tat
T25921 1220-1222 CC denotes or
T25922 1223-1231 JJ denotes FLAG-Tat
T25923 1232-1233 NN denotes R
T25924 1233-1234 -LRB- denotes (
T25925 1234-1236 CD denotes 49
T25926 1237-1239 CD denotes 57
T25927 1239-1240 -RRB- denotes )
T25928 1240-1241 DT denotes A
T25929 1242-1243 -LRB- denotes (
T25930 1243-1244 CD denotes 5
T25931 1245-1248 CC denotes and
T25932 1249-1251 CD denotes 10
T25933 1252-1254 NN denotes µl
T25934 1254-1255 -RRB- denotes )
T25935 1255-1256 : denotes ;
T25936 1257-1268 NN denotes competition
T25937 1269-1271 IN denotes of
T25938 1272-1275 NNP denotes DNA
T25939 1276-1283 JJ denotes binding
T25940 1284-1287 VBD denotes was
T25941 1288-1297 VBN denotes performed
T25942 1298-1302 IN denotes with
T25943 1303-1305 CD denotes 10
T25944 1306-1307 : denotes -
T25945 1308-1310 RP denotes up
T25946 1311-1313 TO denotes to
T25947 1314-1322 JJ denotes 100-fold
T25948 1323-1328 NN denotes molar
T25949 1329-1335 NN denotes excess
T25950 1336-1338 IN denotes of
T25951 1339-1348 JJ denotes unlabeled
T25952 1349-1354 JJ denotes NF-κB
T25953 1355-1370 JJ denotes double-stranded
T25954 1371-1386 NN denotes oligonucleotide
T25955 1386-1387 . denotes .
T25956 1388-1399 NNP denotes DNA/protein
T25957 1400-1409 NNS denotes complexes
T25958 1410-1414 VBD denotes were
T25959 1415-1418 VBN denotes run
T25960 1419-1421 IN denotes on
T25961 1422-1423 CD denotes 6
T25962 1423-1424 NN denotes %
T25963 1425-1429 NN denotes PAGE
T25964 1430-1433 NNP denotes TBE
T25965 1434-1437 CC denotes and
T25966 1438-1446 VBN denotes analysed
T25967 1447-1449 IN denotes by
T25968 1450-1465 NN denotes autoradiography
T25969 1465-1466 . denotes .
T25970 1467-1468 -LRB- denotes (
T25971 1468-1469 NNP denotes E
T25972 1469-1470 -RRB- denotes )
T25973 1471-1474 FW denotes p50
T25974 1474-1475 FW denotes −
T25975 1475-1476 FW denotes /
T25976 1476-1477 FW denotes −
T25977 1477-1480 FW denotes p65
T25978 1480-1481 FW denotes −
T25979 1481-1482 FW denotes /
T25980 1482-1483 FW denotes −
T25981 1483-1487 NNS denotes MEFs
T25982 1488-1489 -LRB- denotes (
T25983 1489-1490 CD denotes 3
T25984 1491-1492 CD denotes ×
T25985 1493-1496 CD denotes 105
T25986 1497-1502 NNS denotes cells
T25987 1502-1503 -RRB- denotes )
T25988 1504-1508 VBD denotes were
T25989 1509-1520 VBN denotes transfected
T25990 1521-1525 IN denotes with
T25991 1526-1532 NNP denotes pκBLuc
T25992 1533-1534 -LRB- denotes (
T25993 1534-1537 CD denotes 0.5
T25994 1538-1540 NN denotes µg
T25995 1540-1541 -RRB- denotes )
T25996 1542-1545 CC denotes and
T25997 1546-1555 JJ denotes pSV-β-Gal
T25998 1556-1557 -LRB- denotes (
T25999 1557-1560 CD denotes 0.1
T26000 1561-1563 NN denotes µg
T26001 1563-1564 -RRB- denotes )
T26002 1565-1569 IN denotes with
T26003 1570-1572 CC denotes or
T26004 1573-1580 IN denotes without
T26005 1581-1590 NNP denotes pRc/CMV-p
T26006 1590-1592 CD denotes 65
T26007 1593-1594 -LRB- denotes (
T26008 1594-1597 CD denotes 0.5
T26009 1598-1600 NN denotes µg
T26010 1600-1601 -RRB- denotes )
T26011 1601-1602 , denotes ,
T26012 1603-1617 NN denotes pCMV4-HA-IκB-α
T26013 1618-1619 -LRB- denotes (
T26014 1619-1622 CD denotes 0.5
T26015 1623-1625 NN denotes µg
T26016 1625-1626 -RRB- denotes )
T26017 1626-1627 , denotes ,
T26018 1628-1639 NN denotes p3xFLAG-Tat
T26019 1640-1642 CC denotes or
T26020 1643-1654 NN denotes p3xFLAG-Tat
T26021 1655-1656 NN denotes R
T26022 1656-1657 -LRB- denotes (
T26023 1657-1659 CD denotes 49
T26024 1660-1662 CD denotes 57
T26025 1662-1663 -RRB- denotes )
T26026 1663-1664 DT denotes A
T26027 1665-1666 -LRB- denotes (
T26028 1666-1669 CD denotes 0.5
T26029 1669-1670 , denotes ,
T26030 1671-1672 CD denotes 1
T26031 1673-1676 CC denotes and
T26032 1677-1678 CD denotes 2
T26033 1679-1681 NN denotes µg
T26034 1681-1682 -RRB- denotes )
T26035 1682-1683 . denotes .
T26036 1684-1687 DT denotes The
T26037 1688-1698 NN denotes luciferase
T26038 1699-1707 NN denotes activity
T26039 1708-1711 VBD denotes was
T26040 1712-1720 VBN denotes measured
T26041 1721-1723 IN denotes in
T26042 1724-1728 NN denotes cell
T26043 1729-1737 NNS denotes extracts
T26044 1738-1742 JJ denotes 48-h
T26045 1743-1760 NN denotes post-transfection
T26046 1761-1764 CC denotes and
T26047 1765-1775 VBN denotes normalized
T26048 1776-1778 TO denotes to
T26049 1779-1794 JJ denotes β-galactosidase
T26050 1795-1803 NN denotes activity
T26051 1803-1804 . denotes .
T26052 1805-1809 VB denotes Fold
T26053 1810-1820 NN denotes activation
T26054 1821-1824 VBD denotes was
T26055 1825-1835 VBN denotes calculated
T26056 1836-1844 JJ denotes relative
T26057 1845-1847 TO denotes to
T26058 1848-1860 NN denotes transfection
T26059 1861-1863 IN denotes of
T26060 1864-1867 DT denotes the
T26061 1868-1874 NNP denotes pκBLuc
T26062 1875-1882 NN denotes plasmid
T26063 1883-1888 RB denotes alone
T26064 1888-1889 . denotes .
T26065 1890-1896 NNS denotes Values
T26066 1897-1898 -LRB- denotes (
T26067 1898-1902 JJ denotes mean
T26068 1903-1904 NN denotes ±
T26069 1905-1907 NNP denotes SE
T26070 1907-1908 , denotes ,
T26071 1909-1910 JJ denotes n
T26072 1911-1912 SYM denotes =
T26073 1913-1914 LS denotes 3
T26074 1914-1915 -RRB- denotes )
T26075 1916-1919 VBP denotes are
T26076 1920-1925 VBN denotes shown
T26077 1925-1926 . denotes .
T26078 1927-1929 IN denotes As
T26079 1930-1937 NN denotes control
T26080 1938-1940 IN denotes of
T26081 1941-1948 NN denotes protein
T26082 1949-1959 NN denotes expression
T26083 1959-1960 , denotes ,
T26084 1961-1969 NNS denotes aliquots
T26085 1970-1972 IN denotes of
T26086 1973-1977 NN denotes cell
T26087 1978-1986 NNS denotes extracts
T26088 1987-1988 -LRB- denotes (
T26089 1988-1990 CD denotes 20
T26090 1991-1993 NN denotes µg
T26091 1993-1994 -RRB- denotes )
T26092 1995-1999 VBD denotes were
T26093 2000-2008 VBN denotes analysed
T26094 2009-2011 IN denotes by
T26095 2012-2019 JJ denotes western
T26096 2020-2028 VBG denotes blotting
T26097 2029-2033 IN denotes with
T26098 2034-2042 JJ denotes anti-p65
T26099 2042-2043 , denotes ,
T26100 2044-2051 JJ denotes anti-HA
T26101 2051-2052 , denotes ,
T26102 2053-2062 JJ denotes anti-FLAG
T26103 2063-2066 CC denotes and
T26104 2067-2081 JJ denotes anti-γ-tubulin
T26105 2082-2092 NNS denotes antibodies
T26106 2092-2093 . denotes .
R20432 T25677 T25678 nsubj Tat,relieves
R20433 T25678 T25678 ROOT relieves,relieves
R20434 T25679 T25678 dobj p65,relieves
R20435 T25680 T25678 prep from,relieves
R20436 T25681 T25683 det the,inhibition
R20437 T25682 T25683 amod IκB-α,inhibition
R20438 T25683 T25680 pobj inhibition,from
R20439 T25684 T25678 punct .,relieves
R20440 T25685 T25686 punct (,A
R20441 T25686 T25689 nmod A,representation
R20442 T25687 T25686 punct ),A
R20443 T25688 T25689 amod Schematic,representation
R20444 T25689 T25689 ROOT representation,representation
R20445 T25690 T25689 prep of,representation
R20446 T25691 T25695 amod wild-type,proteins
R20447 T25692 T25691 cc and,wild-type
R20448 T25693 T25691 conj mutant,wild-type
R20449 T25694 T25695 compound Tat,proteins
R20450 T25695 T25690 pobj proteins,of
R20451 T25696 T25689 punct .,representation
R20452 T25697 T25700 punct (,HA-IκB-α
R20453 T25698 T25700 nmod B,HA-IκB-α
R20454 T25699 T25700 punct ),HA-IκB-α
R20455 T25700 T25706 nsubjpass HA-IκB-α,incubated
R20456 T25701 T25703 punct (,µl
R20457 T25702 T25703 nummod 5,µl
R20458 T25703 T25700 appos µl,HA-IκB-α
R20459 T25704 T25703 punct ),µl
R20460 T25705 T25706 auxpass was,incubated
R20461 T25706 T25706 ROOT incubated,incubated
R20462 T25707 T25706 prep in,incubated
R20463 T25708 T25707 pobj presence,in
R20464 T25709 T25708 cc or,presence
R20465 T25710 T25708 conj absence,presence
R20466 T25711 T25710 prep of,absence
R20467 T25712 T25711 pobj FLAG-Tat,of
R20468 T25713 T25712 punct ",",FLAG-Tat
R20469 T25714 T25715 compound FLAG-Tat,T
R20470 T25715 T25712 conj T,FLAG-Tat
R20471 T25716 T25715 punct ",",T
R20472 T25717 T25767 punct ;,separated
R20473 T25718 T25715 conj N,T
R20474 T25719 T25720 punct (,"23,24"
R20475 T25720 T25718 parataxis "23,24",N
R20476 T25721 T25720 punct ),"23,24"
R20477 T25722 T25725 det A,K
R20478 T25723 T25725 punct ",",K
R20479 T25724 T25725 compound FLAG-Tat,K
R20480 T25725 T25725 ROOT K,K
R20481 T25726 T25725 punct (,K
R20482 T25727 T25725 appos "50,51",K
R20483 T25728 T25725 punct ),K
R20484 T25729 T25732 det A,R
R20485 T25730 T25732 punct ",",R
R20486 T25731 T25732 amod FLAG-Tat,R
R20487 T25732 T25732 ROOT R,R
R20488 T25733 T25732 punct (,R
R20489 T25734 T25732 appos 49,R
R20490 T25735 T25732 appos 57,R
R20491 T25736 T25732 punct ),R
R20492 T25737 T25740 det A,C
R20493 T25738 T25739 cc or,FLAG-Tat
R20494 T25739 T25740 compound FLAG-Tat,C
R20495 T25740 T25740 ROOT C,C
R20496 T25741 T25740 punct (,C
R20497 T25742 T25740 appos "22,25,27",C
R20498 T25743 T25740 punct ),C
R20499 T25744 T25747 det A,µl
R20500 T25745 T25747 punct (,µl
R20501 T25746 T25747 nummod 10,µl
R20502 T25747 T25747 ROOT µl,µl
R20503 T25748 T25747 punct ),µl
R20504 T25749 T25747 acl using,µl
R20505 T25750 T25749 prep in,using
R20506 T25751 T25753 amod vitro,proteins
R20507 T25752 T25753 amod translated,proteins
R20508 T25753 T25750 pobj proteins,in
R20509 T25754 T25747 punct .,µl
R20510 T25755 T25759 amod Wild-type,proteins
R20511 T25756 T25755 cc and,Wild-type
R20512 T25757 T25755 conj mutant,Wild-type
R20513 T25758 T25759 compound Tat,proteins
R20514 T25759 T25761 nsubjpass proteins,immunoprecipitated
R20515 T25760 T25761 auxpass were,immunoprecipitated
R20516 T25761 T25767 ccomp immunoprecipitated,separated
R20517 T25762 T25761 prep with,immunoprecipitated
R20518 T25763 T25764 amod anti-FLAG,antibody
R20519 T25764 T25762 pobj antibody,with
R20520 T25765 T25767 nsubjpass immunocomplexes,separated
R20521 T25766 T25767 auxpass were,separated
R20522 T25767 T25767 ROOT separated,separated
R20523 T25768 T25767 prep by,separated
R20524 T25769 T25770 nummod 12,%
R20525 T25770 T25768 pobj %,by
R20526 T25771 T25772 compound SDS,PAGE
R20527 T25772 T25767 dobj PAGE,separated
R20528 T25773 T25772 cc and,PAGE
R20529 T25774 T25772 conj analysed,PAGE
R20530 T25775 T25774 agent by,analysed
R20531 T25776 T25777 amod western,blotting
R20532 T25777 T25775 pobj blotting,by
R20533 T25778 T25777 prep with,blotting
R20534 T25779 T25782 amod anti-HA,antibodies
R20535 T25780 T25779 cc and,anti-HA
R20536 T25781 T25779 conj anti-FLAG,anti-HA
R20537 T25782 T25778 pobj antibodies,with
R20538 T25783 T25767 punct .,separated
R20539 T25784 T25785 punct (,C
R20540 T25785 T25788 nmod C,p65
R20541 T25786 T25785 punct ),C
R20542 T25787 T25788 amod 35S-methionine-labeled,p65
R20543 T25788 T25794 nsubjpass p65,incubated
R20544 T25789 T25788 punct (,p65
R20545 T25790 T25791 nummod 5,µl
R20546 T25791 T25788 appos µl,p65
R20547 T25792 T25791 punct ),µl
R20548 T25793 T25794 auxpass was,incubated
R20549 T25794 T25794 ROOT incubated,incubated
R20550 T25795 T25794 prep with,incubated
R20551 T25796 T25798 prep in,translated
R20552 T25797 T25798 nsubj vitro,translated
R20553 T25798 T25794 conj translated,incubated
R20554 T25799 T25798 dobj HA-IκB-α,translated
R20555 T25800 T25802 punct (,µl
R20556 T25801 T25802 nummod 5,µl
R20557 T25802 T25799 appos µl,HA-IκB-α
R20558 T25803 T25802 punct ),µl
R20559 T25804 T25798 prep in,translated
R20560 T25805 T25804 pobj presence,in
R20561 T25806 T25805 cc or,presence
R20562 T25807 T25805 conj absence,presence
R20563 T25808 T25807 prep of,absence
R20564 T25809 T25808 pobj FLAG-Tat,of
R20565 T25810 T25809 cc or,FLAG-Tat
R20566 T25811 T25812 compound FLAG-Tat,R
R20567 T25812 T25809 conj R,FLAG-Tat
R20568 T25813 T25809 punct (,FLAG-Tat
R20569 T25814 T25809 appos 49,FLAG-Tat
R20570 T25815 T25809 appos 57,FLAG-Tat
R20571 T25816 T25809 punct ),FLAG-Tat
R20572 T25817 T25824 det A,µl
R20573 T25818 T25824 punct (,µl
R20574 T25819 T25824 nummod 5,µl
R20575 T25820 T25824 punct ",",µl
R20576 T25821 T25824 nummod 10,µl
R20577 T25822 T25821 cc or,10
R20578 T25823 T25821 conj 20,10
R20579 T25824 T25824 ROOT µl,µl
R20580 T25825 T25824 punct ),µl
R20581 T25826 T25824 punct .,µl
R20582 T25827 T25829 nsubjpass HA-IκB-α,immunoprecipitated
R20583 T25828 T25829 auxpass was,immunoprecipitated
R20584 T25829 T25836 ccomp immunoprecipitated,separated
R20585 T25830 T25829 prep with,immunoprecipitated
R20586 T25831 T25832 amod anti-HA,antibody
R20587 T25832 T25830 pobj antibody,with
R20588 T25833 T25836 punct ;,separated
R20589 T25834 T25836 nsubjpass immunocomplexes,separated
R20590 T25835 T25836 auxpass were,separated
R20591 T25836 T25836 ROOT separated,separated
R20592 T25837 T25836 prep by,separated
R20593 T25838 T25839 nummod 12,%
R20594 T25839 T25840 compound %,SDS
R20595 T25840 T25837 pobj SDS,by
R20596 T25841 T25840 conj PAGE,SDS
R20597 T25842 T25836 cc and,separated
R20598 T25843 T25836 conj analysed,separated
R20599 T25844 T25843 agent by,analysed
R20600 T25845 T25846 amod western,blotting
R20601 T25846 T25844 pobj blotting,by
R20602 T25847 T25846 prep with,blotting
R20603 T25848 T25851 amod anti-HA,antibodies
R20604 T25849 T25848 cc and,anti-HA
R20605 T25850 T25848 conj anti-FLAG,anti-HA
R20606 T25851 T25847 pobj antibodies,with
R20607 T25852 T25851 punct ",",antibodies
R20608 T25853 T25851 cc or,antibodies
R20609 T25854 T25851 conj autoradiography,antibodies
R20610 T25855 T25856 punct (,35S-Met-p65
R20611 T25856 T25854 appos 35S-Met-p65,autoradiography
R20612 T25857 T25843 punct ),analysed
R20613 T25858 T25836 punct .,separated
R20614 T25859 T25860 compound Densitometry,values
R20615 T25860 T25868 nsubjpass values,expressed
R20616 T25861 T25862 punct (,D
R20617 T25862 T25860 appos D,values
R20618 T25863 T25860 punct ),values
R20619 T25864 T25860 prep of,values
R20620 T25865 T25866 det the,bands
R20621 T25866 T25864 pobj bands,of
R20622 T25867 T25868 auxpass were,expressed
R20623 T25868 T25868 ROOT expressed,expressed
R20624 T25869 T25870 mark as,fold
R20625 T25870 T25868 advcl fold,expressed
R20626 T25871 T25870 dobj increase,fold
R20627 T25872 T25870 prep above,fold
R20628 T25873 T25874 det the,control
R20629 T25874 T25872 pobj control,above
R20630 T25875 T25874 punct (,control
R20631 T25876 T25874 appos lane,control
R20632 T25877 T25876 nummod 1,lane
R20633 T25878 T25874 punct ),control
R20634 T25879 T25868 punct .,expressed
R20635 T25880 T25881 punct (,D
R20636 T25881 T25881 ROOT D,D
R20637 T25882 T25881 punct ),D
R20638 T25883 T25885 det The,DNA
R20639 T25884 T25885 nummod p65,DNA
R20640 T25885 T25887 compound DNA,activity
R20641 T25886 T25887 amod binding,activity
R20642 T25887 T25889 nsubjpass activity,analysed
R20643 T25888 T25889 auxpass was,analysed
R20644 T25889 T25889 ROOT analysed,analysed
R20645 T25890 T25889 agent by,analysed
R20646 T25891 T25890 pobj EMSA,by
R20647 T25892 T25889 advcl using,analysed
R20648 T25893 T25892 prt in,using
R20649 T25894 T25896 amod vitro,proteins
R20650 T25895 T25896 amod translated,proteins
R20651 T25896 T25893 pobj proteins,in
R20652 T25897 T25889 punct .,analysed
R20653 T25898 T25900 amod 32P-labeled-NF-κB,oligonucleotide
R20654 T25899 T25900 amod double-stranded,oligonucleotide
R20655 T25900 T25902 nsubjpass oligonucleotide,incubated
R20656 T25901 T25902 auxpass was,incubated
R20657 T25902 T25902 ROOT incubated,incubated
R20658 T25903 T25902 prep with,incubated
R20659 T25904 T25903 pobj p65,with
R20660 T25905 T25907 punct (,µl
R20661 T25906 T25907 nummod 0.5,µl
R20662 T25907 T25904 appos µl,p65
R20663 T25908 T25907 punct ),µl
R20664 T25909 T25907 prep in,µl
R20665 T25910 T25909 pobj presence,in
R20666 T25911 T25910 cc or,presence
R20667 T25912 T25910 conj absence,presence
R20668 T25913 T25912 prep of,absence
R20669 T25914 T25923 nmod HA-IκB-α,R
R20670 T25915 T25917 punct (,µl
R20671 T25916 T25917 nummod 1,µl
R20672 T25917 T25914 appos µl,HA-IκB-α
R20673 T25918 T25917 punct ),µl
R20674 T25919 T25917 punct ",",µl
R20675 T25920 T25923 amod FLAG-Tat,R
R20676 T25921 T25920 cc or,FLAG-Tat
R20677 T25922 T25920 conj FLAG-Tat,FLAG-Tat
R20678 T25923 T25913 pobj R,of
R20679 T25924 T25923 punct (,R
R20680 T25925 T25923 appos 49,R
R20681 T25926 T25923 appos 57,R
R20682 T25927 T25923 punct ),R
R20683 T25928 T25933 det A,µl
R20684 T25929 T25930 punct (,5
R20685 T25930 T25933 nummod 5,µl
R20686 T25931 T25930 cc and,5
R20687 T25932 T25930 conj 10,5
R20688 T25933 T25941 nsubjpass µl,performed
R20689 T25934 T25933 punct ),µl
R20690 T25935 T25936 punct ;,competition
R20691 T25936 T25941 nsubjpass competition,performed
R20692 T25937 T25936 prep of,competition
R20693 T25938 T25937 pobj DNA,of
R20694 T25939 T25941 nsubjpass binding,performed
R20695 T25940 T25941 auxpass was,performed
R20696 T25941 T25941 ROOT performed,performed
R20697 T25942 T25941 prep with,performed
R20698 T25943 T25949 nummod 10,excess
R20699 T25944 T25945 punct -,up
R20700 T25945 T25949 nmod up,excess
R20701 T25946 T25947 quantmod to,100-fold
R20702 T25947 T25949 nmod 100-fold,excess
R20703 T25948 T25949 compound molar,excess
R20704 T25949 T25942 pobj excess,with
R20705 T25950 T25949 prep of,excess
R20706 T25951 T25954 amod unlabeled,oligonucleotide
R20707 T25952 T25954 amod NF-κB,oligonucleotide
R20708 T25953 T25954 amod double-stranded,oligonucleotide
R20709 T25954 T25950 pobj oligonucleotide,of
R20710 T25955 T25941 punct .,performed
R20711 T25956 T25957 compound DNA/protein,complexes
R20712 T25957 T25959 nsubjpass complexes,run
R20713 T25958 T25959 auxpass were,run
R20714 T25959 T25959 ROOT run,run
R20715 T25960 T25959 prep on,run
R20716 T25961 T25962 nummod 6,%
R20717 T25962 T25963 compound %,PAGE
R20718 T25963 T25960 pobj PAGE,on
R20719 T25964 T25963 appos TBE,PAGE
R20720 T25965 T25959 cc and,run
R20721 T25966 T25959 conj analysed,run
R20722 T25967 T25966 agent by,analysed
R20723 T25968 T25967 pobj autoradiography,by
R20724 T25969 T25959 punct .,run
R20725 T25970 T25989 punct (,transfected
R20726 T25971 T25980 dep E,−
R20727 T25972 T25973 punct ),p50
R20728 T25973 T25980 dep p50,−
R20729 T25974 T25980 dep −,−
R20730 T25975 T25980 dep /,−
R20731 T25976 T25980 dep −,−
R20732 T25977 T25980 dep p65,−
R20733 T25978 T25980 dep −,−
R20734 T25979 T25980 dep /,−
R20735 T25980 T25981 nmod −,MEFs
R20736 T25981 T25989 nsubjpass MEFs,transfected
R20737 T25982 T25981 punct (,MEFs
R20738 T25983 T25986 nummod 3,cells
R20739 T25984 T25986 nummod ×,cells
R20740 T25985 T25986 nummod 105,cells
R20741 T25986 T25981 appos cells,MEFs
R20742 T25987 T25981 punct ),MEFs
R20743 T25988 T25989 auxpass were,transfected
R20744 T25989 T25989 ROOT transfected,transfected
R20745 T25990 T25989 prep with,transfected
R20746 T25991 T25990 pobj pκBLuc,with
R20747 T25992 T25994 punct (,µg
R20748 T25993 T25994 nummod 0.5,µg
R20749 T25994 T25991 appos µg,pκBLuc
R20750 T25995 T25994 punct ),µg
R20751 T25996 T25991 cc and,pκBLuc
R20752 T25997 T25991 conj pSV-β-Gal,pκBLuc
R20753 T25998 T26000 punct (,µg
R20754 T25999 T26000 nummod 0.1,µg
R20755 T26000 T25997 appos µg,pSV-β-Gal
R20756 T26001 T26000 punct ),µg
R20757 T26002 T25997 prep with,pSV-β-Gal
R20758 T26003 T26002 cc or,with
R20759 T26004 T26002 conj without,with
R20760 T26005 T26004 pobj pRc/CMV-p,without
R20761 T26006 T26005 nummod 65,pRc/CMV-p
R20762 T26007 T26009 punct (,µg
R20763 T26008 T26009 nummod 0.5,µg
R20764 T26009 T26005 appos µg,pRc/CMV-p
R20765 T26010 T26009 punct ),µg
R20766 T26011 T26005 punct ",",pRc/CMV-p
R20767 T26012 T26005 appos pCMV4-HA-IκB-α,pRc/CMV-p
R20768 T26013 T26015 punct (,µg
R20769 T26014 T26015 nummod 0.5,µg
R20770 T26015 T26012 appos µg,pCMV4-HA-IκB-α
R20771 T26016 T26015 punct ),µg
R20772 T26017 T26015 punct ",",µg
R20773 T26018 T26015 conj p3xFLAG-Tat,µg
R20774 T26019 T26018 cc or,p3xFLAG-Tat
R20775 T26020 T26018 conj p3xFLAG-Tat,p3xFLAG-Tat
R20776 T26021 T26004 pobj R,without
R20777 T26022 T26024 punct (,57
R20778 T26023 T26024 nummod 49,57
R20779 T26024 T26021 appos 57,R
R20780 T26025 T26021 punct ),R
R20781 T26026 T26033 det A,µg
R20782 T26027 T26033 punct (,µg
R20783 T26028 T26033 nummod 0.5,µg
R20784 T26029 T26030 punct ",",1
R20785 T26030 T26028 prep 1,0.5
R20786 T26031 T26028 cc and,0.5
R20787 T26032 T26028 conj 2,0.5
R20788 T26033 T26033 ROOT µg,µg
R20789 T26034 T26033 punct ),µg
R20790 T26035 T26033 punct .,µg
R20791 T26036 T26038 det The,activity
R20792 T26037 T26038 compound luciferase,activity
R20793 T26038 T26040 nsubjpass activity,measured
R20794 T26039 T26040 auxpass was,measured
R20795 T26040 T26040 ROOT measured,measured
R20796 T26041 T26040 prep in,measured
R20797 T26042 T26043 compound cell,extracts
R20798 T26043 T26041 pobj extracts,in
R20799 T26044 T26045 compound 48-h,post-transfection
R20800 T26045 T26043 appos post-transfection,extracts
R20801 T26046 T26045 cc and,post-transfection
R20802 T26047 T26045 conj normalized,post-transfection
R20803 T26048 T26047 prep to,normalized
R20804 T26049 T26050 amod β-galactosidase,activity
R20805 T26050 T26048 pobj activity,to
R20806 T26051 T26040 punct .,measured
R20807 T26052 T26053 compound Fold,activation
R20808 T26053 T26055 nsubjpass activation,calculated
R20809 T26054 T26055 auxpass was,calculated
R20810 T26055 T26055 ROOT calculated,calculated
R20811 T26056 T26055 oprd relative,calculated
R20812 T26057 T26056 prep to,relative
R20813 T26058 T26057 pobj transfection,to
R20814 T26059 T26058 prep of,transfection
R20815 T26060 T26062 det the,plasmid
R20816 T26061 T26062 compound pκBLuc,plasmid
R20817 T26062 T26059 pobj plasmid,of
R20818 T26063 T26058 advmod alone,transfection
R20819 T26064 T26055 punct .,calculated
R20820 T26065 T26076 nsubjpass Values,shown
R20821 T26066 T26069 punct (,SE
R20822 T26067 T26069 amod mean,SE
R20823 T26068 T26069 compound ±,SE
R20824 T26069 T26065 appos SE,Values
R20825 T26070 T26069 punct ",",SE
R20826 T26071 T26073 nmod n,3
R20827 T26072 T26073 punct =,3
R20828 T26073 T26069 appos 3,SE
R20829 T26074 T26069 punct ),SE
R20830 T26075 T26076 auxpass are,shown
R20831 T26076 T26076 ROOT shown,shown
R20832 T26077 T26076 punct .,shown
R20833 T26078 T26093 prep As,analysed
R20834 T26079 T26078 pobj control,As
R20835 T26080 T26079 prep of,control
R20836 T26081 T26082 compound protein,expression
R20837 T26082 T26080 pobj expression,of
R20838 T26083 T26093 punct ",",analysed
R20839 T26084 T26093 nsubjpass aliquots,analysed
R20840 T26085 T26084 prep of,aliquots
R20841 T26086 T26087 compound cell,extracts
R20842 T26087 T26085 pobj extracts,of
R20843 T26088 T26087 punct (,extracts
R20844 T26089 T26090 nummod 20,µg
R20845 T26090 T26087 appos µg,extracts
R20846 T26091 T26087 punct ),extracts
R20847 T26092 T26093 auxpass were,analysed
R20848 T26093 T26093 ROOT analysed,analysed
R20849 T26094 T26093 agent by,analysed
R20850 T26095 T26096 amod western,blotting
R20851 T26096 T26094 pobj blotting,by
R20852 T26097 T26096 prep with,blotting
R20853 T26098 T26105 amod anti-p65,antibodies
R20854 T26099 T26098 punct ",",anti-p65
R20855 T26100 T26105 amod anti-HA,antibodies
R20856 T26101 T26100 punct ",",anti-HA
R20857 T26102 T26100 conj anti-FLAG,anti-HA
R20858 T26103 T26102 cc and,anti-FLAG
R20859 T26104 T26102 conj anti-γ-tubulin,anti-FLAG
R20860 T26105 T26097 pobj antibodies,with
R20861 T26106 T26093 punct .,analysed

GO-BP

Id Subject Object Predicate Lexical cue
T26107 300-310 http://purl.obolibrary.org/obo/GO_0006412 denotes translated
T26108 589-599 http://purl.obolibrary.org/obo/GO_0006412 denotes translated
T26109 300-319 http://purl.obolibrary.org/obo/GO_0006412 denotes translated proteins
T26110 1065-1084 http://purl.obolibrary.org/obo/GO_0006412 denotes translated proteins
T26111 1688-1707 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T26112 1688-1707 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T26113 1688-1707 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T26114 1688-1707 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T26115 1688-1707 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T26116 1779-1803 http://purl.obolibrary.org/obo/GO_0004565 denotes β-galactosidase activity
T26117 1779-1803 http://purl.obolibrary.org/obo/GO_0004336 denotes β-galactosidase activity
T26118 1779-1803 http://purl.obolibrary.org/obo/GO_1903771 denotes β-galactosidase activity
T26119 1779-1803 http://purl.obolibrary.org/obo/GO_1903770 denotes β-galactosidase activity
T26120 1779-1803 http://purl.obolibrary.org/obo/GO_0033920 denotes β-galactosidase activity
T26121 1779-1803 http://purl.obolibrary.org/obo/GO_0033929 denotes β-galactosidase activity
T26122 1779-1803 http://purl.obolibrary.org/obo/GO_0033930 denotes β-galactosidase activity

GO-MF

Id Subject Object Predicate Lexical cue
T26123 394-402 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T26124 736-744 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T26125 853-863 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T26126 2082-2092 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T26127 1008-1019 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T26128 1272-1283 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T26129 1012-1019 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T26130 1276-1283 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T26131 1688-1707 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T26132 1688-1707 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T26133 1688-1707 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T26134 1688-1707 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T26135 1688-1707 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T26136 1779-1803 http://purl.obolibrary.org/obo/GO_0004565 denotes β-galactosidase activity
T26137 1779-1803 http://purl.obolibrary.org/obo/GO_0004336 denotes β-galactosidase activity
T26138 1779-1803 http://purl.obolibrary.org/obo/GO_0033920 denotes β-galactosidase activity
T26139 1779-1803 http://purl.obolibrary.org/obo/GO_0033929 denotes β-galactosidase activity
T26140 1779-1803 http://purl.obolibrary.org/obo/GO_0033930 denotes β-galactosidase activity

GO-CC

Id Subject Object Predicate Lexical cue
T26141 394-402 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T26142 736-744 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T26143 853-863 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T26144 2082-2092 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T26145 394-402 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T26146 736-744 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T26147 853-863 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T26148 2082-2092 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T26149 1388-1409 http://purl.obolibrary.org/obo/GO_0001114 denotes DNA/protein complexes
T26150 1388-1409 http://purl.obolibrary.org/obo/GO_0032993 denotes DNA/protein complexes
T26151 1388-1409 http://purl.obolibrary.org/obo/GO_0097522 denotes DNA/protein complexes
T26152 1392-1409 http://purl.obolibrary.org/obo/GO_0043234 denotes protein complexes
T26153 1497-1502 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26154 1724-1728 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T26155 1973-1977 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T25204 10-320 Sentence denotes Tat relieves p65 from the IκB-α inhibition. (A) Schematic representation of wild-type and mutant Tat proteins. (B) HA-IκB-α (5 µl) was incubated in presence or absence of FLAG-Tat, FLAG-Tat T,N(23,24)A, FLAG-Tat K(50,51)A, FLAG-Tat R(49–57)A or FLAG-Tat C(22,25,27)A (10 µl) using in vitro translated proteins.
T25205 321-690 Sentence denotes Wild-type and mutant Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies. (C) 35S-methionine-labeled p65 (5 µl) was incubated with in vitro translated HA-IκB-α (5 µl) in presence or absence of FLAG-Tat or FLAG-Tat R(49–57)A (5, 10 or 20 µl).
T25206 691-898 Sentence denotes HA-IκB-α was immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies, or autoradiography (35S-Met-p65).
T25207 899-1085 Sentence denotes Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1). (D) The p65 DNA binding activity was analysed by EMSA using in vitro translated proteins.
T25208 1086-1387 Sentence denotes 32P-labeled-NF-κB double-stranded oligonucleotide was incubated with p65 (0.5 µl) in presence or absence of HA-IκB-α (1 µl), FLAG-Tat or FLAG-Tat R(49–57)A (5 and 10 µl); competition of DNA binding was performed with 10 - up to 100-fold molar excess of unlabeled NF-κB double-stranded oligonucleotide.
T25209 1388-1683 Sentence denotes DNA/protein complexes were run on 6% PAGE–TBE and analysed by autoradiography. (E) p50−/−p65−/−MEFs (3 × 105 cells) were transfected with pκBLuc (0.5 µg) and pSV-β-Gal (0.1 µg) with or without pRc/CMV-p65 (0.5 µg), pCMV4-HA-IκB-α (0.5 µg), p3xFLAG-Tat or p3xFLAG-Tat R(49–57)A (0.5, 1 and 2 µg).
T25210 1684-1804 Sentence denotes The luciferase activity was measured in cell extracts 48-h post-transfection and normalized to β-galactosidase activity.
T25211 1805-1889 Sentence denotes Fold activation was calculated relative to transfection of the pκBLuc plasmid alone.
T25212 1890-1926 Sentence denotes Values (mean ± SE, n = 3) are shown.
T25213 1927-2093 Sentence denotes As control of protein expression, aliquots of cell extracts (20 µg) were analysed by western blotting with anti-p65, anti-HA, anti-FLAG and anti-γ-tubulin antibodies.
T149 0-9 Sentence denotes Figure 3.
T150 10-320 Sentence denotes Tat relieves p65 from the IκB-α inhibition. (A) Schematic representation of wild-type and mutant Tat proteins. (B) HA-IκB-α (5 µl) was incubated in presence or absence of FLAG-Tat, FLAG-Tat T,N(23,24)A, FLAG-Tat K(50,51)A, FLAG-Tat R(49–57)A or FLAG-Tat C(22,25,27)A (10 µl) using in vitro translated proteins.
T151 321-690 Sentence denotes Wild-type and mutant Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies. (C) 35S-methionine-labeled p65 (5 µl) was incubated with in vitro translated HA-IκB-α (5 µl) in presence or absence of FLAG-Tat or FLAG-Tat R(49–57)A (5, 10 or 20 µl).
T152 691-898 Sentence denotes HA-IκB-α was immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies, or autoradiography (35S-Met-p65).
T153 899-1085 Sentence denotes Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1). (D) The p65 DNA binding activity was analysed by EMSA using in vitro translated proteins.
T154 1086-1387 Sentence denotes 32P-labeled-NF-κB double-stranded oligonucleotide was incubated with p65 (0.5 µl) in presence or absence of HA-IκB-α (1 µl), FLAG-Tat or FLAG-Tat R(49–57)A (5 and 10 µl); competition of DNA binding was performed with 10 - up to 100-fold molar excess of unlabeled NF-κB double-stranded oligonucleotide.
T155 1388-1683 Sentence denotes DNA/protein complexes were run on 6% PAGE–TBE and analysed by autoradiography. (E) p50−/−p65−/−MEFs (3 × 105 cells) were transfected with pκBLuc (0.5 µg) and pSV-β-Gal (0.1 µg) with or without pRc/CMV-p65 (0.5 µg), pCMV4-HA-IκB-α (0.5 µg), p3xFLAG-Tat or p3xFLAG-Tat R(49–57)A (0.5, 1 and 2 µg).
T156 1684-1804 Sentence denotes The luciferase activity was measured in cell extracts 48-h post-transfection and normalized to β-galactosidase activity.
T157 1805-1889 Sentence denotes Fold activation was calculated relative to transfection of the pκBLuc plasmid alone.
T158 1890-1926 Sentence denotes Values (mean ± SE, n = 3) are shown.
T159 1927-2093 Sentence denotes As control of protein expression, aliquots of cell extracts (20 µg) were analysed by western blotting with anti-p65, anti-HA, anti-FLAG and anti-γ-tubulin antibodies.

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T26333 10-13 Protein denotes Tat
T26334 14-22 Negative_regulation denotes relieves
T26335 23-26 Protein denotes p65
T26336 36-41 Protein denotes IκB-α
T26337 42-52 Negative_regulation denotes inhibition
T26338 107-110 Protein denotes Tat
T26339 125-133 Protein denotes HA-IκB-α
T26340 181-189 Protein denotes FLAG-Tat
T26341 191-211 Protein denotes FLAG-Tat T,N(23,24)A
T26342 213-231 Protein denotes FLAG-Tat K(50,51)A
T26343 233-251 Protein denotes FLAG-Tat R(49–57)A
T26344 255-276 Protein denotes FLAG-Tat C(22,25,27)A
T26345 342-345 Protein denotes Tat
T26346 550-553 Protein denotes p65
T26347 600-608 Protein denotes HA-IκB-α
T26348 642-650 Protein denotes FLAG-Tat
T26349 654-672 Protein denotes FLAG-Tat R(49–57)A
T26350 691-699 Protein denotes HA-IκB-α
T26351 893-896 Protein denotes p65
T26352 1004-1007 Protein denotes p65
T26353 1012-1019 Binding denotes binding true
T26354 1155-1158 Protein denotes p65
T26355 1194-1202 Protein denotes HA-IκB-α
T26356 1211-1219 Protein denotes FLAG-Tat
T26357 1223-1241 Protein denotes FLAG-Tat R(49–57)A
T26358 1257-1268 Negative_regulation denotes competition true
T26359 1276-1283 Binding denotes binding
T26360 1471-1474 Protein denotes p50
T26361 1477-1480 Protein denotes p65
T26362 1550-1555 Protein denotes β-Gal
T26363 1589-1592 Protein denotes p65
T26364 1609-1617 Protein denotes HA-IκB-α
T26365 1636-1639 Protein denotes Tat
T26366 1651-1654 Protein denotes Tat
T26367 1688-1698 Protein denotes luciferase
T26368 1779-1794 Protein denotes β-galactosidase
R20896 T26333 T26334 causeOf Tat,relieves
R20897 T26335 T26337 themeOf p65,inhibition
R20898 T26336 T26337 causeOf IκB-α,inhibition
R20899 T26337 T26334 themeOf inhibition,relieves
R20900 T26352 T26353 themeOf p65,binding
R20901 T26354 T26359 themeOf p65,binding
R20902 T26355 T26358 causeOf HA-IκB-α,competition
R20903 T26359 T26358 themeOf binding,competition

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T26369 23-26 Protein denotes p65
T26370 36-41 Protein denotes IκB-α
T26371 42-52 Negative_regulation denotes inhibition
T26372 14-22 Negative_regulation denotes relieves
T26373 100-119 Protein denotes mutant Tat proteins
T26374 125-127 Protein denotes HA
T26375 128-133 Protein denotes IκB-α
T26376 181-189 Protein denotes FLAG-Tat
T26377 191-195 Protein denotes FLAG
T26378 196-199 Protein denotes Tat
T26379 335-354 Protein denotes mutant Tat proteins
T26380 550-553 Protein denotes p65
T26381 600-602 Protein denotes HA
T26382 603-608 Protein denotes IκB-α
T26383 642-646 Protein denotes FLAG
T26384 647-650 Protein denotes Tat
T26385 654-658 Protein denotes FLAG
T26386 659-662 Protein denotes Tat
T26387 691-693 Protein denotes HA
T26388 694-699 Protein denotes IκB-α
T26389 728-744 Protein denotes anti-HA antibody
T26390 1004-1011 Protein denotes p65 DNA
T26391 1012-1019 Binding denotes binding
T26392 1101-1103 Protein denotes κB
T26393 1194-1196 Protein denotes HA
T26394 1197-1200 Protein denotes IκB
T26395 1211-1219 Protein denotes FLAG-Tat
T26396 1223-1227 Protein denotes FLAG
T26397 1228-1231 Protein denotes Tat
T26398 1349-1354 Protein denotes NF-κB
T26399 1603-1617 Protein denotes pCMV4-HA-IκB-α
T26400 1628-1639 Protein denotes p3xFLAG-Tat
T26401 1643-1650 Protein denotes p3xFLAG
T26402 1651-1654 Protein denotes Tat
T26403 1688-1698 Protein denotes luciferase
T26404 1779-1794 Protein denotes β-galactosidase
T26405 1868-1888 Protein denotes pκBLuc plasmid alone
T26406 1848-1860 Gene_expression denotes transfection
T26407 2039-2042 Protein denotes p65
T26408 2044-2051 Protein denotes anti-HA
T26409 2053-2062 Protein denotes anti-FLAG
T26410 2067-2092 Protein denotes anti-γ-tubulin antibodies
R20904 T26369 T26372 themeOf p65,relieves
R20905 T26370 T26371 themeOf IκB-α,inhibition
R20906 T26371 T26372 causeOf inhibition,relieves
R20907 T26390 T26391 themeOf p65 DNA,binding
R20908 T26405 T26406 themeOf pκBLuc plasmid alone,transfection

test2

Id Subject Object Predicate Lexical cue Speculation
T25130 10-13 Protein denotes Tat
T25131 14-22 Negative_regulation denotes relieves
T25132 23-26 Protein denotes p65 true
T25133 36-41 Protein denotes IκB-α true
T25134 42-52 Negative_regulation denotes inhibition
T25135 107-110 Protein denotes Tat
T25136 125-133 Protein denotes HA-IκB-α
T25137 170-177 Negative_regulation denotes absence
T25138 181-189 Protein denotes FLAG-Tat
T25139 191-211 Protein denotes FLAG-Tat T,N(23,24)A
T25140 213-231 Protein denotes FLAG-Tat K(50,51)A
T25141 233-251 Protein denotes FLAG-Tat R(49–57)A
T25142 255-276 Protein denotes FLAG-Tat C(22,25,27)A
T25143 342-345 Protein denotes Tat
T25144 550-553 Protein denotes p65
T25145 600-608 Protein denotes HA-IκB-α
T25146 631-638 Negative_regulation denotes absence
T25147 642-650 Protein denotes FLAG-Tat
T25148 654-672 Protein denotes FLAG-Tat R(49–57)A
T25149 691-699 Protein denotes HA-IκB-α
T25150 893-896 Protein denotes p65
T25151 1004-1007 Protein denotes p65
T25152 1012-1019 Binding denotes binding
T25153 1155-1158 Protein denotes p65
T25154 1183-1190 Negative_regulation denotes absence
T25155 1194-1202 Protein denotes HA-IκB-α
T25156 1211-1219 Protein denotes FLAG-Tat
T25157 1223-1241 Protein denotes FLAG-Tat R(49–57)A
T25158 1276-1283 Binding denotes binding
T25159 1471-1474 Protein denotes p50
T25160 1477-1480 Protein denotes p65
T25161 1550-1555 Protein denotes β-Gal
T25162 1589-1592 Protein denotes p65
T25163 1609-1617 Protein denotes HA-IκB-α
T25164 1636-1639 Protein denotes Tat
T25165 1651-1654 Protein denotes Tat
T25166 1688-1698 Protein denotes luciferase
T25167 1779-1794 Protein denotes β-galactosidase
R20015 T25130 T25131 causeOf Tat,relieves
R20016 T25132 T25134 themeOf p65,inhibition
R20017 T25133 T25134 causeOf IκB-α,inhibition
R20018 T25134 T25131 themeOf inhibition,relieves
R20019 T25147 T25146 themeOf FLAG-Tat,absence
R20020 T25151 T25152 themeOf p65,binding
R20021 T25155 T25158 themeOf HA-IκB-α,binding
R20022 T25155 T25154 themeOf HA-IκB-α,absence
R20023 T25156 T25158 themeOf FLAG-Tat,binding
R20024 T25157 T25158 themeOf FLAG-Tat R(49–57)A,binding

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T25194 1276-1283 Binding denotes binding
T25195 1471-1474 Protein denotes p50
T25196 1477-1480 Protein denotes p65
T25197 1550-1555 Protein denotes β-Gal
T25198 1589-1592 Protein denotes p65
T25199 1609-1617 Protein denotes HA-IκB-α
T25200 1636-1639 Protein denotes Tat
T25201 1651-1654 Protein denotes Tat
T25202 1688-1698 Protein denotes luciferase
T25203 1779-1794 Protein denotes β-galactosidase
T25168 10-13 Protein denotes Tat
T25169 14-22 Negative_regulation denotes relieves
T25170 23-26 Protein denotes p65
T25171 36-41 Protein denotes IκB-α
T25172 42-52 Negative_regulation denotes inhibition
T25173 107-110 Protein denotes Tat
T25174 125-133 Protein denotes HA-IκB-α
T25175 181-189 Protein denotes FLAG-Tat
T25176 191-211 Protein denotes FLAG-Tat T,N(23,24)A
T25177 213-231 Protein denotes FLAG-Tat K(50,51)A
T25178 233-251 Protein denotes FLAG-Tat R(49–57)A
T25179 255-276 Protein denotes FLAG-Tat C(22,25,27)A
T25180 342-345 Protein denotes Tat
T25181 550-553 Protein denotes p65
T25182 600-608 Protein denotes HA-IκB-α
T25183 642-650 Protein denotes FLAG-Tat
T25184 654-672 Protein denotes FLAG-Tat R(49–57)A
T25185 691-699 Protein denotes HA-IκB-α
T25186 893-896 Protein denotes p65
T25187 1004-1007 Protein denotes p65
T25188 1012-1019 Binding denotes binding true
T25189 1155-1158 Protein denotes p65
T25190 1194-1202 Protein denotes HA-IκB-α
T25191 1211-1219 Protein denotes FLAG-Tat
T25192 1223-1241 Protein denotes FLAG-Tat R(49–57)A
T25193 1257-1268 Negative_regulation denotes competition true
R20025 T25168 T25169 causeOf Tat,relieves
R20026 T25170 T25172 themeOf p65,inhibition
R20027 T25171 T25172 causeOf IκB-α,inhibition
R20028 T25172 T25169 themeOf inhibition,relieves
R20029 T25187 T25188 themeOf p65,binding
R20030 T25189 T25194 themeOf p65,binding
R20031 T25190 T25193 causeOf HA-IκB-α,competition
R20032 T25194 T25193 themeOf binding,competition

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T25592 10-13 P04608 denotes Tat
T25593 10-13 Q8UMQ1 denotes Tat
T25594 10-13 P04610 denotes Tat
T25595 10-13 P04605 denotes Tat
T25596 23-26 Q04206 denotes p65
T25597 23-26 P21579 denotes p65
T25598 107-110 P04605 denotes Tat
T25599 107-110 Q8UMQ1 denotes Tat
T25600 107-110 P04610 denotes Tat
T25601 107-110 P04608 denotes Tat
T25602 186-189 P04605 denotes Tat
T25603 186-189 P04608 denotes Tat
T25604 186-189 Q8UMQ1 denotes Tat
T25605 186-189 P04610 denotes Tat
T25606 196-199 Q8UMQ1 denotes Tat
T25607 196-199 P04610 denotes Tat
T25608 196-199 P04608 denotes Tat
T25609 196-199 P04605 denotes Tat
T25610 218-221 Q8UMQ1 denotes Tat
T25611 218-221 P04610 denotes Tat
T25612 218-221 P04608 denotes Tat
T25613 218-221 P04605 denotes Tat
T25614 238-241 P04610 denotes Tat
T25615 238-241 P04608 denotes Tat
T25616 238-241 P04605 denotes Tat
T25617 238-241 Q8UMQ1 denotes Tat
T25618 260-263 P04610 denotes Tat
T25619 260-263 Q8UMQ1 denotes Tat
T25620 260-263 P04608 denotes Tat
T25621 260-263 P04605 denotes Tat
T25622 342-345 Q8UMQ1 denotes Tat
T25623 342-345 P04608 denotes Tat
T25624 342-345 P04610 denotes Tat
T25625 342-345 P04605 denotes Tat
T25626 550-553 P21579 denotes p65
T25627 550-553 Q04206 denotes p65
T25628 647-650 Q8UMQ1 denotes Tat
T25629 647-650 P04605 denotes Tat
T25630 647-650 P04608 denotes Tat
T25631 647-650 P04610 denotes Tat
T25632 659-662 P04610 denotes Tat
T25633 659-662 P04608 denotes Tat
T25634 659-662 P04605 denotes Tat
T25635 659-662 Q8UMQ1 denotes Tat
T25636 893-896 P21579 denotes p65
T25637 893-896 Q04206 denotes p65
T25638 1004-1007 Q04206 denotes p65
T25639 1004-1007 P21579 denotes p65
T25640 1155-1158 P21579 denotes p65
T25641 1155-1158 Q04206 denotes p65
T25642 1216-1219 P04610 denotes Tat
T25643 1216-1219 Q8UMQ1 denotes Tat
T25644 1216-1219 P04608 denotes Tat
T25645 1216-1219 P04605 denotes Tat
T25646 1228-1231 Q8UMQ1 denotes Tat
T25647 1228-1231 P04608 denotes Tat
T25648 1228-1231 P04610 denotes Tat
T25649 1228-1231 P04605 denotes Tat
T25650 1471-1474 P19838 denotes p50
T25651 1477-1480 Q04206 denotes p65
T25652 1477-1480 P21579 denotes p65
T25653 1589-1592 Q04206 denotes p65
T25654 1589-1592 P21579 denotes p65
T25655 1636-1639 Q8UMQ1 denotes Tat
T25656 1636-1639 P04608 denotes Tat
T25657 1636-1639 P04610 denotes Tat
T25658 1636-1639 P04605 denotes Tat
T25659 1651-1654 Q8UMQ1 denotes Tat
T25660 1651-1654 P04610 denotes Tat
T25661 1651-1654 P04608 denotes Tat
T25662 1651-1654 P04605 denotes Tat
T25663 1688-1698 P08659 denotes luciferase
T25664 1836-1844 Q04864 denotes relative
T25665 2039-2042 P21579 denotes p65
T25666 2039-2042 Q04206 denotes p65