PMC:3333881 / 22496-26457 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
22187158-17942396-77584449	598-600	17942396	denotes	50
22187158-21664225-77584450	601-603	21664225	denotes	51

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T11613	0-3	NN	denotes	Tat
T11614	4-15	VB	denotes	counteracts
T11615	16-19	DT	denotes	the
T11616	20-25	JJ	denotes	IκB-α
T11617	26-36	NN	denotes	inhibition
T11618	37-39	IN	denotes	of
T11619	40-43	NN	denotes	p65
T11620	44-46	IN	denotes	by
T11621	47-56	VB	denotes	competing
T11622	57-60	DT	denotes	the
T11623	61-70	NN	denotes	repressor
T11624	71-78	NN	denotes	binding
T11625	79-81	PRP	denotes	We
T11626	82-89	RB	denotes	further
T11627	90-102	VB	denotes	investigated
T11628	103-106	DT	denotes	the
T11629	107-115	JJ	denotes	physical
T11630	116-127	NN	denotes	interaction
T11631	128-130	IN	denotes	of
T11632	131-134	NN	denotes	Tat
T11633	135-139	IN	denotes	with
T11634	140-145	NN	denotes	IκB-α
T11635	146-151	VB	denotes	using
T11636	152-154	FW	denotes	in
T11637	155-160	FW	denotes	vitro
T11638	161-171	VB	denotes	translated
T11639	172-180	NN	denotes	proteins
T11640	181-183	IN	denotes	in
T11641	184-205	NN	denotes	coimmunoprecipitation
T11642	206-212	NN	denotes	assays
T11643	214-217	IN	denotes	For
T11644	218-225	VB	denotes	mapping
T11645	226-229	DT	denotes	the
T11646	230-241	NN	denotes	interaction
T11647	242-249	NN	denotes	domains
T11648	249-250	-COMMA-	denotes	,
T11649	251-253	PRP	denotes	we
T11650	254-258	VB	denotes	used
T11651	259-262	DT	denotes	the
T11652	263-272	VB	denotes	following
T11653	273-276	NN	denotes	Tat
T11654	277-284	NN	denotes	mutants
T11655	284-285	-COLON-	denotes	:
T11656	286-289	NN	denotes	Tat
T11657	290-291	NNP	denotes	T
T11658	291-292	-COMMA-	denotes	,
T11659	292-293	NN	denotes	N
T11660	293-294	-LRB-	denotes	(
T11661	294-299	CD	denotes	23,24
T11662	299-300	-RRB-	denotes	)
T11663	300-301	NN	denotes	A
T11664	301-302	-COMMA-	denotes	,
T11665	303-306	NN	denotes	Tat
T11666	307-308	NN	denotes	C
T11667	308-309	-LRB-	denotes	(
T11668	309-317	CD	denotes	22,25,27
T11669	317-318	-RRB-	denotes	)
T11670	318-319	NN	denotes	A
T11671	319-320	-COMMA-	denotes	,
T11672	321-324	NN	denotes	Tat
T11673	325-326	NN	denotes	K
T11674	326-327	-LRB-	denotes	(
T11675	327-332	CD	denotes	50,51
T11676	332-333	-RRB-	denotes	)
T11677	333-334	NN	denotes	A
T11678	335-338	CC	denotes	and
T11679	339-342	NN	denotes	Tat
T11680	343-344	NN	denotes	R
T11681	344-345	-LRB-	denotes	(
T11682	345-350	CD	denotes	49–57
T11683	350-351	-RRB-	denotes	)
T11684	351-352	NN	denotes	A
T11685	353-354	-LRB-	denotes	(
T11686	354-360	NN	denotes	Figure
T11687	361-363	NN	denotes	3A
T11688	363-364	-RRB-	denotes	)
T11689	366-371	NN	denotes	IκB-α
T11690	372-392	VB	denotes	coimmunoprecipitated
T11691	393-397	IN	denotes	with
T11692	398-401	DT	denotes	all
T11693	402-405	NN	denotes	Tat
T11694	406-414	NN	denotes	proteins
T11695	414-415	-COMMA-	denotes	,
T11696	416-422	IN	denotes	except
T11697	423-426	NN	denotes	Tat
T11698	427-428	NN	denotes	R
T11699	428-429	-LRB-	denotes	(
T11700	429-434	CD	denotes	49–57
T11701	434-435	-RRB-	denotes	)
T11702	435-436	NN	denotes	A
T11703	437-438	-LRB-	denotes	(
T11704	438-444	NN	denotes	Figure
T11705	445-447	NN	denotes	3B
T11706	447-448	-RRB-	denotes	)
T11707	450-455	DT	denotes	These
T11708	456-463	NN	denotes	results
T11709	464-473	VB	denotes	indicated
T11710	474-478	IN	denotes	that
T11711	479-482	DT	denotes	the
T11712	483-496	JJ	denotes	arginine-rich
T11713	497-503	NN	denotes	domain
T11714	504-506	IN	denotes	of
T11715	507-510	NN	denotes	Tat
T11716	511-514	VB	denotes	was
T11717	515-523	VB	denotes	involved
T11718	524-526	IN	denotes	in
T11719	527-530	DT	denotes	the
T11720	531-538	NN	denotes	binding
T11721	539-541	TO	denotes	to
T11722	542-547	NN	denotes	IκB-α
T11723	547-548	-COMMA-	denotes	,
T11724	549-554	WDT	denotes	which
T11725	555-558	VB	denotes	was
T11726	559-569	JJ	denotes	consistent
T11727	570-574	IN	denotes	with
T11728	575-583	JJ	denotes	previous
T11729	584-596	NN	denotes	observations
T11730	597-598	-LRB-	denotes	(
T11731	598-603	CD	denotes	50,51
T11732	603-604	-RRB-	denotes	)
T11733	606-614	RB	denotes	Moreover
T11734	614-615	-COMMA-	denotes	,
T11735	616-619	NN	denotes	Tat
T11736	620-631	VB	denotes	associating
T11737	632-636	IN	denotes	with
T11738	637-642	NN	denotes	IκB-α
T11739	643-651	VB	denotes	competed
T11740	652-655	DT	denotes	the
T11741	656-663	NN	denotes	binding
T11742	664-666	IN	denotes	of
T11743	667-672	NN	denotes	IκB-α
T11744	673-675	TO	denotes	to
T11745	676-679	NN	denotes	p65
T11746	680-682	IN	denotes	in
T11747	683-684	DT	denotes	a
T11748	685-699	JJ	denotes	dose-dependent
T11749	700-706	NN	denotes	manner
T11750	707-708	-LRB-	denotes	(
T11751	708-714	NN	denotes	Figure
T11752	715-717	NN	denotes	3C
T11753	717-718	-COMMA-	denotes	,
T11754	719-724	NN	denotes	lanes
T11755	725-728	CD	denotes	3–5
T11756	728-729	-RRB-	denotes	)
T11757	729-730	-COMMA-	denotes	,
T11758	731-736	IN	denotes	while
T11759	737-740	NN	denotes	Tat
T11760	741-742	NN	denotes	R
T11761	742-743	-LRB-	denotes	(
T11762	743-748	CD	denotes	49–57
T11763	748-749	-RRB-	denotes	)
T11764	749-750	NN	denotes	A
T11765	750-751	-COMMA-	denotes	,
T11766	752-757	WDT	denotes	which
T11767	758-764	VB	denotes	lacked
T11768	765-768	DT	denotes	the
T11769	769-776	VB	denotes	binding
T11770	777-781	NN	denotes	site
T11771	782-785	IN	denotes	for
T11772	786-791	NN	denotes	IκB-α
T11773	791-792	-COMMA-	denotes	,
T11774	793-796	VB	denotes	did
T11775	797-800	RB	denotes	not
T11776	801-810	VB	denotes	associate
T11777	811-815	IN	denotes	with
T11778	816-821	NN	denotes	IκB-α
T11779	822-825	CC	denotes	and
T11780	826-829	VB	denotes	did
T11781	830-833	RB	denotes	not
T11782	834-841	VB	denotes	compete
T11783	842-845	DT	denotes	the
T11784	846-853	NN	denotes	binding
T11785	854-856	IN	denotes	of
T11786	857-862	NN	denotes	IκB-α
T11787	863-865	TO	denotes	to
T11788	866-869	NN	denotes	p65
T11789	870-871	-LRB-	denotes	(
T11790	871-877	NN	denotes	Figure
T11791	878-880	NN	denotes	3C
T11792	880-881	-COMMA-	denotes	,
T11793	882-887	NN	denotes	lanes
T11794	888-891	CD	denotes	6–8
T11795	891-892	-RRB-	denotes	)
T11796	2988-2992	RB	denotes	Next
T11797	2992-2993	-COMMA-	denotes	,
T11798	2994-2996	PRP	denotes	we
T11799	2997-3006	VB	denotes	evaluated
T11800	3007-3014	IN	denotes	whether
T11801	3015-3018	NN	denotes	Tat
T11802	3019-3031	VB	denotes	counteracted
T11803	3032-3035	DT	denotes	the
T11804	3036-3050	JJ	denotes	IκB-α-mediated
T11805	3051-3061	NN	denotes	inhibition
T11806	3062-3064	IN	denotes	of
T11807	3065-3068	NN	denotes	p65
T11808	3069-3076	NN	denotes	binding
T11809	3077-3079	TO	denotes	to
T11810	3080-3083	NN	denotes	DNA
T11811	3084-3086	IN	denotes	by
T11812	3087-3097	VB	denotes	incubating
T11813	3098-3100	FW	denotes	in
T11814	3101-3106	FW	denotes	vitro
T11815	3107-3117	VB	denotes	translated
T11816	3118-3121	NN	denotes	p65
T11817	3122-3125	CC	denotes	and
T11818	3126-3131	NN	denotes	IκB-α
T11819	3132-3140	NN	denotes	proteins
T11820	3141-3145	IN	denotes	with
T11821	3146-3149	DT	denotes	the
T11822	3150-3155	NN	denotes	NF-κB
T11823	3156-3161	NN	denotes	probe
T11824	3162-3164	IN	denotes	in
T11825	3165-3173	NN	denotes	presence
T11826	3174-3176	CC	denotes	or
T11827	3177-3184	NN	denotes	absence
T11828	3185-3187	IN	denotes	of
T11829	3188-3191	NN	denotes	Tat
T11830	3192-3200	VB	denotes	followed
T11831	3201-3203	IN	denotes	by
T11832	3204-3208	NN	denotes	EMSA
T11833	3210-3213	DT	denotes	The
T11834	3214-3217	NN	denotes	p65
T11835	3218-3221	NN	denotes	DNA
T11836	3222-3229	NN	denotes	binding
T11837	3230-3238	NN	denotes	activity
T11838	3239-3242	VB	denotes	was
T11839	3243-3252	VB	denotes	inhibited
T11840	3253-3255	IN	denotes	by
T11841	3256-3261	NN	denotes	IκB-α
T11842	3262-3265	CC	denotes	and
T11843	3266-3274	VB	denotes	restored
T11844	3275-3277	IN	denotes	in
T11845	3278-3279	DT	denotes	a
T11846	3280-3294	JJ	denotes	dose-dependent
T11847	3295-3301	NN	denotes	manner
T11848	3302-3304	IN	denotes	by
T11849	3305-3314	JJ	denotes	wild-type
T11850	3315-3318	NN	denotes	Tat
T11851	3318-3319	-COMMA-	denotes	,
T11852	3320-3323	CC	denotes	and
T11853	3324-3327	RB	denotes	not
T11854	3328-3330	IN	denotes	by
T11855	3331-3334	NN	denotes	Tat
T11856	3335-3336	NN	denotes	R
T11857	3336-3337	-LRB-	denotes	(
T11858	3337-3342	CD	denotes	49–57
T11859	3342-3343	-RRB-	denotes	)
T11860	3344-3345	-LRB-	denotes	(
T11861	3345-3351	NN	denotes	Figure
T11862	3352-3354	NN	denotes	3D
T11863	3354-3355	-RRB-	denotes	)
T11864	3357-3364	RB	denotes	Further
T11865	3364-3365	-COMMA-	denotes	,
T11866	3366-3368	PRP	denotes	we
T11867	3369-3377	VB	denotes	analysed
T11868	3378-3381	DT	denotes	the
T11869	3382-3385	NN	denotes	Tat
T11870	3386-3392	NN	denotes	effect
T11871	3393-3395	IN	denotes	on
T11872	3396-3399	DT	denotes	the
T11873	3400-3405	JJ	denotes	IκB-α
T11874	3406-3416	NN	denotes	repression
T11875	3417-3419	IN	denotes	of
T11876	3420-3423	NN	denotes	p65
T11877	3424-3439	JJ	denotes	transcriptional
T11878	3440-3448	NN	denotes	activity
T11879	3449-3451	IN	denotes	by
T11880	3452-3464	VB	denotes	transfecting
T11881	3465-3481	NN	denotes	p50−/−p65−/−MEFs
T11882	3482-3486	IN	denotes	with
T11883	3487-3490	DT	denotes	the
T11884	3491-3500	NN	denotes	NF-κB-Luc
T11885	3501-3509	NN	denotes	reporter
T11886	3510-3518	RB	denotes	together
T11887	3519-3523	IN	denotes	with
T11888	3524-3534	NN	denotes	expression
T11889	3535-3542	NN	denotes	vectors
T11890	3543-3545	IN	denotes	of
T11891	3546-3549	NN	denotes	p65
T11892	3550-3553	CC	denotes	and
T11893	3554-3559	NN	denotes	IκB-α
T11894	3559-3560	-COMMA-	denotes	,
T11895	3561-3563	IN	denotes	in
T11896	3564-3572	NN	denotes	presence
T11897	3573-3575	CC	denotes	or
T11898	3576-3583	NN	denotes	absence
T11899	3584-3586	IN	denotes	of
T11900	3587-3591	NNP	denotes	Tat.
T11901	3592-3597	NNP	denotes	IκB-α
T11902	3598-3607	VB	denotes	inhibited
T11903	3608-3611	DT	denotes	the
T11904	3612-3625	JJ	denotes	p65-dependent
T11905	3626-3636	NN	denotes	expression
T11906	3637-3639	IN	denotes	of
T11907	3640-3643	DT	denotes	the
T11908	3644-3654	NN	denotes	luciferase
T11909	3655-3659	NN	denotes	gene
T11910	3659-3660	-COMMA-	denotes	,
T11911	3661-3666	WDT	denotes	which
T11912	3667-3670	VB	denotes	was
T11913	3671-3679	VB	denotes	restored
T11914	3680-3682	IN	denotes	in
T11915	3683-3684	DT	denotes	a
T11916	3685-3699	JJ	denotes	dose-dependent
T11917	3700-3706	NN	denotes	manner
T11918	3707-3709	IN	denotes	by
T11919	3710-3719	JJ	denotes	wild-type
T11920	3720-3723	NN	denotes	Tat
T11921	3723-3724	-COMMA-	denotes	,
T11922	3725-3728	CC	denotes	and
T11923	3729-3732	RB	denotes	not
T11924	3733-3735	IN	denotes	by
T11925	3736-3739	NN	denotes	Tat
T11926	3740-3741	NN	denotes	R
T11927	3741-3742	-LRB-	denotes	(
T11928	3742-3747	CD	denotes	49–57
T11929	3747-3748	-RRB-	denotes	)
T11930	3748-3749	NN	denotes	A
T11931	3750-3751	-LRB-	denotes	(
T11932	3751-3757	NN	denotes	Figure
T11933	3758-3760	NN	denotes	3E
T11934	3760-3761	-RRB-	denotes	)
T11935	3763-3773	RB	denotes	Altogether
T11936	3774-3779	DT	denotes	these
T11937	3780-3787	NN	denotes	results
T11938	3788-3797	VB	denotes	indicated
T11939	3798-3802	IN	denotes	that
T11940	3803-3806	NN	denotes	Tat
T11941	3807-3818	VB	denotes	counteracts
T11942	3819-3822	DT	denotes	the
T11943	3823-3828	JJ	denotes	IκB-α
T11944	3829-3839	NN	denotes	repression
T11945	3840-3842	IN	denotes	of
T11946	3843-3846	DT	denotes	the
T11947	3847-3850	NN	denotes	p65
T11948	3851-3862	JJ	denotes	DNA-binding
T11949	3863-3866	CC	denotes	and
T11950	3867-3882	JJ	denotes	transcriptional
T11951	3883-3891	NN	denotes	activity
T11952	3892-3894	IN	denotes	by
T11953	3895-3906	VB	denotes	associating
T11954	3907-3911	IN	denotes	with
T11955	3912-3917	NN	denotes	IκB-α
T11956	3918-3921	CC	denotes	and
T11957	3922-3931	VB	denotes	competing
T11958	3932-3935	DT	denotes	the
T11959	3936-3945	NN	denotes	repressor
T11960	3946-3953	NN	denotes	binding
T11961	3954-3956	TO	denotes	to
T11962	3957-3960	NN	denotes	p65
T25214	904-907	NN	denotes	Tat
T25215	908-916	VB	denotes	relieves
T25216	917-920	NN	denotes	p65
T25217	921-925	IN	denotes	from
T25218	926-929	DT	denotes	the
T25219	930-935	JJ	denotes	IκB-α
T25220	936-947	NN	denotes	inhibition.
T25221	948-949	-LRB-	denotes	(
T25222	949-950	NN	denotes	A
T25223	950-951	-RRB-	denotes	)
T25224	952-961	JJ	denotes	Schematic
T25225	962-976	NN	denotes	representation
T25226	977-979	IN	denotes	of
T25227	980-989	JJ	denotes	wild-type
T25228	990-993	CC	denotes	and
T25229	994-1000	JJ	denotes	mutant
T25230	1001-1004	NN	denotes	Tat
T25231	1005-1014	NN	denotes	proteins.
T25232	1015-1016	-LRB-	denotes	(
T25233	1016-1017	NN	denotes	B
T25234	1017-1018	-RRB-	denotes	)
T25235	1019-1027	NN	denotes	HA-IκB-α
T25236	1028-1029	-LRB-	denotes	(
T25237	1029-1033	NN	denotes	5 µl
T25238	1033-1034	-RRB-	denotes	)
T25239	1035-1038	VB	denotes	was
T25240	1039-1048	VB	denotes	incubated
T25241	1049-1051	IN	denotes	in
T25242	1052-1060	NN	denotes	presence
T25243	1061-1063	CC	denotes	or
T25244	1064-1071	NN	denotes	absence
T25245	1072-1074	IN	denotes	of
T25246	1075-1083	NN	denotes	FLAG-Tat
T25247	1083-1084	-COMMA-	denotes	,
T25248	1085-1093	NN	denotes	FLAG-Tat
T25249	1094-1095	NNP	denotes	T
T25250	1095-1096	-COMMA-	denotes	,
T25251	1096-1097	NN	denotes	N
T25252	1097-1098	-LRB-	denotes	(
T25253	1098-1103	CD	denotes	23,24
T25254	1103-1104	-RRB-	denotes	)
T25255	1104-1105	NN	denotes	A
T25256	1105-1106	-COMMA-	denotes	,
T25257	1107-1115	NN	denotes	FLAG-Tat
T25258	1116-1117	NN	denotes	K
T25259	1117-1118	-LRB-	denotes	(
T25260	1118-1123	CD	denotes	50,51
T25261	1123-1124	-RRB-	denotes	)
T25262	1124-1125	NN	denotes	A
T25263	1125-1126	-COMMA-	denotes	,
T25264	1127-1135	NN	denotes	FLAG-Tat
T25265	1136-1137	NN	denotes	R
T25266	1137-1138	-LRB-	denotes	(
T25267	1138-1143	CD	denotes	49–57
T25268	1143-1144	-RRB-	denotes	)
T25269	1144-1145	NN	denotes	A
T25270	1146-1148	CC	denotes	or
T25271	1149-1157	NN	denotes	FLAG-Tat
T25272	1158-1159	NN	denotes	C
T25273	1159-1160	-LRB-	denotes	(
T25274	1160-1168	CD	denotes	22,25,27
T25275	1168-1169	-RRB-	denotes	)
T25276	1169-1170	NN	denotes	A
T25277	1171-1172	-LRB-	denotes	(
T25278	1172-1177	NN	denotes	10 µl
T25279	1177-1178	-RRB-	denotes	)
T25280	1179-1184	VB	denotes	using
T25281	1185-1187	FW	denotes	in
T25282	1188-1193	FW	denotes	vitro
T25283	1194-1204	VB	denotes	translated
T25284	1205-1213	NN	denotes	proteins
T25285	1215-1224	JJ	denotes	Wild-type
T25286	1225-1228	CC	denotes	and
T25287	1229-1235	JJ	denotes	mutant
T25288	1236-1239	NN	denotes	Tat
T25289	1240-1248	NN	denotes	proteins
T25290	1249-1253	VB	denotes	were
T25291	1254-1272	VB	denotes	immunoprecipitated
T25292	1273-1277	IN	denotes	with
T25293	1278-1287	JJ	denotes	anti-FLAG
T25294	1288-1296	NN	denotes	antibody
T25295	1296-1297	-COLON-	denotes	;
T25296	1298-1313	NN	denotes	immunocomplexes
T25297	1314-1318	VB	denotes	were
T25298	1319-1328	VB	denotes	separated
T25299	1329-1331	IN	denotes	by
T25300	1332-1334	CD	denotes	12
T25301	1334-1335	NN	denotes	%
T25302	1336-1344	NN	denotes	SDS–PAGE
T25303	1345-1348	CC	denotes	and
T25304	1349-1357	VB	denotes	analysed
T25305	1358-1360	IN	denotes	by
T25306	1361-1368	JJ	denotes	western
T25307	1369-1377	NN	denotes	blotting
T25308	1378-1382	IN	denotes	with
T25309	1383-1390	JJ	denotes	anti-HA
T25310	1391-1394	CC	denotes	and
T25311	1395-1404	JJ	denotes	anti-FLAG
T25312	1405-1416	NN	denotes	antibodies.
T25313	1417-1418	-LRB-	denotes	(
T25314	1418-1419	NN	denotes	C
T25315	1419-1420	-RRB-	denotes	)
T25316	1421-1443	JJ	denotes	35S-methionine-labeled
T25317	1444-1447	NN	denotes	p65
T25318	1448-1449	-LRB-	denotes	(
T25319	1449-1453	NN	denotes	5 µl
T25320	1453-1454	-RRB-	denotes	)
T25321	1455-1458	VB	denotes	was
T25322	1459-1468	VB	denotes	incubated
T25323	1469-1473	IN	denotes	with
T25324	1474-1476	FW	denotes	in
T25325	1477-1482	FW	denotes	vitro
T25326	1483-1493	VB	denotes	translated
T25327	1494-1502	NN	denotes	HA-IκB-α
T25328	1503-1504	-LRB-	denotes	(
T25329	1504-1508	NN	denotes	5 µl
T25330	1508-1509	-RRB-	denotes	)
T25331	1510-1512	IN	denotes	in
T25332	1513-1521	NN	denotes	presence
T25333	1522-1524	CC	denotes	or
T25334	1525-1532	NN	denotes	absence
T25335	1533-1535	IN	denotes	of
T25336	1536-1544	NN	denotes	FLAG-Tat
T25337	1545-1547	CC	denotes	or
T25338	1548-1556	NN	denotes	FLAG-Tat
T25339	1557-1558	NN	denotes	R
T25340	1558-1559	-LRB-	denotes	(
T25341	1559-1564	CD	denotes	49–57
T25342	1564-1565	-RRB-	denotes	)
T25343	1565-1566	NN	denotes	A
T25344	1567-1568	-LRB-	denotes	(
T25345	1568-1569	CD	denotes	5
T25346	1569-1570	-COMMA-	denotes	,
T25347	1571-1573	CD	denotes	10
T25348	1574-1576	CC	denotes	or
T25349	1577-1582	NN	denotes	20 µl
T25350	1582-1583	-RRB-	denotes	)
T25351	1585-1593	NN	denotes	HA-IκB-α
T25352	1594-1597	VB	denotes	was
T25353	1598-1616	VB	denotes	immunoprecipitated
T25354	1617-1621	IN	denotes	with
T25355	1622-1629	JJ	denotes	anti-HA
T25356	1630-1638	NN	denotes	antibody
T25357	1638-1639	-COLON-	denotes	;
T25358	1640-1655	NN	denotes	immunocomplexes
T25359	1656-1660	VB	denotes	were
T25360	1661-1670	VB	denotes	separated
T25361	1671-1673	IN	denotes	by
T25362	1674-1676	CD	denotes	12
T25363	1676-1677	NN	denotes	%
T25364	1678-1686	NN	denotes	SDS–PAGE
T25365	1687-1690	CC	denotes	and
T25366	1691-1699	VB	denotes	analysed
T25367	1700-1702	IN	denotes	by
T25368	1703-1710	JJ	denotes	western
T25369	1711-1719	NN	denotes	blotting
T25370	1720-1724	IN	denotes	with
T25371	1725-1732	JJ	denotes	anti-HA
T25372	1733-1736	CC	denotes	and
T25373	1737-1746	JJ	denotes	anti-FLAG
T25374	1747-1757	NN	denotes	antibodies
T25375	1757-1758	-COMMA-	denotes	,
T25376	1759-1761	CC	denotes	or
T25377	1762-1777	NN	denotes	autoradiography
T25378	1778-1779	-LRB-	denotes	(
T25379	1779-1790	NN	denotes	35S-Met-p65
T25380	1790-1791	-RRB-	denotes	)
T25381	1793-1805	NN	denotes	Densitometry
T25382	1806-1812	NN	denotes	values
T25383	1813-1814	-LRB-	denotes	(
T25384	1814-1815	NN	denotes	D
T25385	1815-1816	-RRB-	denotes	)
T25386	1817-1819	IN	denotes	of
T25387	1820-1823	DT	denotes	the
T25388	1824-1829	NN	denotes	bands
T25389	1830-1834	VB	denotes	were
T25390	1835-1844	VB	denotes	expressed
T25391	1845-1847	IN	denotes	as
T25392	1848-1852	JJ	denotes	fold
T25393	1853-1861	NN	denotes	increase
T25394	1862-1867	IN	denotes	above
T25395	1868-1871	DT	denotes	the
T25396	1872-1879	NN	denotes	control
T25397	1880-1881	-LRB-	denotes	(
T25398	1881-1885	NN	denotes	lane
T25399	1886-1887	CD	denotes	1
T25400	1887-1888	-RRB-	denotes	)
T25401	1888-1889	-LRB-	denotes	.
T25402	1890-1891	NN	denotes	(
T25403	1891-1892	-RRB-	denotes	D
T25404	1892-1895	DT	denotes	) T
T25405	1895-1898	NN	denotes	he
T25406	1898-1901	NN	denotes	p65
T25407	1902-1909	NN	denotes	DNA bin
T25408	1909-1917	NN	denotes	ding act
T25409	1917-1920	VB	denotes	ivi
T25410	1920-1928	VB	denotes	ty was a
T25411	1928-1930	IN	denotes	na
T25412	1930-1934	NN	denotes	lyse
T25413	1934-1939	VB	denotes	d by
T25414	1939-1941	FW	denotes	EM
T25415	1941-1946	FW	denotes	SA us
T25416	1946-1956	VB	denotes	ing in vit
T25417	1956-1964	NN	denotes	ro trans
T25418	1980-1997	NN	denotes	32P-labeled-NF-κB
T25419	1998-2013	JJ	denotes	double-stranded
T25420	2014-2029	NN	denotes	oligonucleotide
T25421	2030-2033	VB	denotes	was
T25422	2034-2043	VB	denotes	incubated
T25423	2044-2048	IN	denotes	with
T25424	2049-2052	NN	denotes	p65
T25425	2053-2054	-LRB-	denotes	(
T25426	2054-2060	NN	denotes	0.5 µl
T25427	2060-2061	-RRB-	denotes	)
T25428	2062-2064	IN	denotes	in
T25429	2065-2073	NN	denotes	presence
T25430	2074-2076	CC	denotes	or
T25431	2077-2084	NN	denotes	absence
T25432	2085-2087	IN	denotes	of
T25433	2088-2096	NN	denotes	HA-IκB-α
T25434	2097-2098	-LRB-	denotes	(
T25435	2098-2102	NN	denotes	1 µl
T25436	2102-2103	-RRB-	denotes	)
T25437	2103-2104	-COMMA-	denotes	,
T25438	2105-2113	NN	denotes	FLAG-Tat
T25439	2114-2116	CC	denotes	or
T25440	2117-2125	NN	denotes	FLAG-Tat
T25441	2126-2127	NN	denotes	R
T25442	2127-2128	-LRB-	denotes	(
T25443	2128-2133	CD	denotes	49–57
T25444	2133-2134	-RRB-	denotes	)
T25445	2134-2135	NN	denotes	A
T25446	2136-2137	-LRB-	denotes	(
T25447	2137-2138	CD	denotes	5
T25448	2139-2142	CC	denotes	and
T25449	2143-2148	NN	denotes	10 µl
T25450	2148-2149	-RRB-	denotes	)
T25451	2149-2150	-COLON-	denotes	;
T25452	2151-2162	NN	denotes	competition
T25453	2163-2165	IN	denotes	of
T25454	2166-2169	NN	denotes	DNA
T25455	2170-2177	NN	denotes	binding
T25456	2178-2181	VB	denotes	was
T25457	2182-2191	VB	denotes	performed
T25458	2192-2196	IN	denotes	with
T25459	2197-2201	CD	denotes	10 -
T25460	2202-2204	IN	denotes	up
T25461	2205-2207	TO	denotes	to
T25462	2208-2216	JJ	denotes	100-fold
T25463	2217-2222	JJ	denotes	molar
T25464	2223-2229	NN	denotes	excess
T25465	2230-2232	IN	denotes	of
T25466	2233-2242	JJ	denotes	unlabeled
T25467	2243-2248	NN	denotes	NF-κB
T25468	2249-2264	JJ	denotes	double-stranded
T25469	2265-2280	NN	denotes	oligonucleotide
T25470	2282-2293	NN	denotes	DNA/protein
T25471	2294-2303	NN	denotes	complexes
T25472	2304-2308	VB	denotes	were
T25473	2309-2312	VB	denotes	run
T25474	2313-2315	IN	denotes	on
T25475	2316-2317	CD	denotes	6
T25476	2317-2318	NN	denotes	%
T25477	2319-2327	NN	denotes	PAGE–TBE
T25478	2328-2331	CC	denotes	and
T25479	2332-2340	VB	denotes	analysed
T25480	2341-2343	IN	denotes	by
T25481	2344-2360	NN	denotes	autoradiography.
T25482	2361-2362	-LRB-	denotes	(
T25483	2362-2363	NN	denotes	E
T25484	2363-2364	-RRB-	denotes	)
T25485	2365-2381	NN	denotes	p50−/−p65−/−MEFs
T25486	2382-2383	-LRB-	denotes	(
T25487	2383-2390	CD	denotes	3 × 105
T25488	2391-2396	NN	denotes	cells
T25489	2396-2397	-RRB-	denotes	)
T25490	2398-2402	VB	denotes	were
T25491	2403-2414	VB	denotes	transfected
T25492	2415-2419	IN	denotes	with
T25493	2420-2426	NN	denotes	pκBLuc
T25494	2427-2428	-LRB-	denotes	(
T25495	2428-2434	NN	denotes	0.5 µg
T25496	2434-2435	-RRB-	denotes	)
T25497	2436-2439	CC	denotes	and
T25498	2440-2449	JJ	denotes	pSV-β-Gal
T25499	2450-2451	-LRB-	denotes	(
T25500	2451-2457	CD	denotes	0.1 µg
T25501	2457-2458	-RRB-	denotes	)
T25502	2459-2463	IN	denotes	with
T25503	2464-2466	CC	denotes	or
T25504	2467-2474	IN	denotes	without
T25505	2475-2486	NN	denotes	pRc/CMV-p65
T25506	2487-2488	-LRB-	denotes	(
T25507	2488-2494	CD	denotes	0.5 µg
T25508	2494-2495	-RRB-	denotes	)
T25509	2495-2496	-COMMA-	denotes	,
T25510	2497-2511	NN	denotes	pCMV4-HA-IκB-α
T25511	2512-2513	-LRB-	denotes	(
T25512	2513-2519	CD	denotes	0.5 µg
T25513	2519-2520	-RRB-	denotes	)
T25514	2520-2521	-COMMA-	denotes	,
T25515	2522-2533	NN	denotes	p3xFLAG-Tat
T25516	2534-2536	CC	denotes	or
T25517	2537-2548	NN	denotes	p3xFLAG-Tat
T25518	2549-2550	NN	denotes	R
T25519	2550-2551	-LRB-	denotes	(
T25520	2551-2556	CD	denotes	49–57
T25521	2556-2557	-RRB-	denotes	)
T25522	2557-2558	NN	denotes	A
T25523	2559-2560	-LRB-	denotes	(
T25524	2560-2563	CD	denotes	0.5
T25525	2563-2564	-COMMA-	denotes	,
T25526	2565-2566	CD	denotes	1
T25527	2567-2570	CC	denotes	and
T25528	2571-2575	NN	denotes	2 µg
T25529	2575-2576	-RRB-	denotes	)
T25530	2578-2581	DT	denotes	The
T25531	2582-2592	NN	denotes	luciferase
T25532	2593-2601	NN	denotes	activity
T25533	2602-2605	VB	denotes	was
T25534	2606-2614	VB	denotes	measured
T25535	2615-2617	IN	denotes	in
T25536	2618-2622	NN	denotes	cell
T25537	2623-2631	NN	denotes	extracts
T25538	2632-2636	JJ	denotes	48-h
T25539	2637-2654	JJ	denotes	post-transfection
T25540	2655-2658	CC	denotes	and
T25541	2659-2669	VB	denotes	normalized
T25542	2670-2672	TO	denotes	to
T25543	2673-2688	JJ	denotes	β-galactosidase
T25544	2689-2697	NN	denotes	activity
T25545	2699-2703	JJ	denotes	Fold
T25546	2704-2714	NN	denotes	activation
T25547	2715-2718	VB	denotes	was
T25548	2719-2729	VB	denotes	calculated
T25549	2730-2738	JJ	denotes	relative
T25550	2739-2741	TO	denotes	to
T25551	2742-2754	NN	denotes	transfection
T25552	2755-2757	IN	denotes	of
T25553	2758-2761	DT	denotes	the
T25554	2762-2768	NN	denotes	pκBLuc
T25555	2769-2776	NN	denotes	plasmid
T25556	2777-2782	RB	denotes	alone
T25557	2784-2790	NN	denotes	Values
T25558	2791-2792	-LRB-	denotes	(
T25559	2792-2801	NN	denotes	mean ± SE
T25560	2801-2802	-COMMA-	denotes	,
T25561	2803-2808	NN	denotes	n = 3
T25562	2808-2809	-RRB-	denotes	)
T25563	2810-2813	VB	denotes	are
T25564	2814-2819	VB	denotes	shown
T25565	2821-2823	IN	denotes	As
T25566	2824-2831	NN	denotes	control
T25567	2832-2834	IN	denotes	of
T25568	2835-2842	NN	denotes	protein
T25569	2843-2853	NN	denotes	expression
T25570	2853-2854	-COMMA-	denotes	,
T25571	2855-2863	NN	denotes	aliquots
T25572	2864-2866	IN	denotes	of
T25573	2867-2871	NN	denotes	cell
T25574	2872-2880	NN	denotes	extracts
T25575	2881-2882	-LRB-	denotes	(
T25576	2882-2887	NN	denotes	20 µg
T25577	2887-2888	-RRB-	denotes	)
T25578	2889-2893	VB	denotes	were
T25579	2894-2902	VB	denotes	analysed
T25580	2903-2905	IN	denotes	by
T25581	2906-2913	JJ	denotes	western
T25582	2914-2922	NN	denotes	blotting
T25583	2923-2927	IN	denotes	with
T25584	2928-2936	JJ	denotes	anti-p65
T25585	2936-2937	-COMMA-	denotes	,
T25586	2938-2945	JJ	denotes	anti-HA
T25587	2945-2946	-COMMA-	denotes	,
T25588	2947-2956	JJ	denotes	anti-FLAG
T25589	2957-2960	CC	denotes	and
T25590	2961-2975	JJ	denotes	anti-γ-tubulin
T25591	2976-2986	NN	denotes	antibodies
R9460	T11613	T11614	arg1Of	Tat,counteracts
R9461	T11613	T11621	arg1Of	Tat,competing
R9462	T11614	T11620	arg1Of	counteracts,by
R9463	T11617	T11614	arg2Of	inhibition,counteracts
R9464	T11617	T11615	arg1Of	inhibition,the
R9465	T11617	T11616	arg1Of	inhibition,IκB-α
R9466	T11617	T11618	arg1Of	inhibition,of
R9467	T11619	T11618	arg2Of	p65,of
R9468	T11621	T11620	arg2Of	competing,by
R9469	T11624	T11621	arg2Of	binding,competing
R9470	T11624	T11622	arg1Of	binding,the
R9471	T11624	T11623	arg1Of	binding,repressor
R9472	T11625	T11627	arg1Of	We,investigated
R9473	T11627	T11626	arg1Of	investigated,further
R9474	T11630	T11627	arg2Of	interaction,investigated
R9475	T11630	T11628	arg1Of	interaction,the
R9476	T11630	T11629	arg1Of	interaction,physical
R9477	T11630	T11631	arg1Of	interaction,of
R9478	T11630	T11633	arg1Of	interaction,with
R9479	T11632	T11631	arg2Of	Tat,of
R9480	T11634	T11633	arg2Of	IκB-α,with
R9481	T11634	T11635	arg1Of	IκB-α,using
R9482	T11635	T11640	arg1Of	using,in
R9483	T11637	T11636	arg1Of	vitro,in
R9484	T11639	T11635	arg2Of	proteins,using
R9485	T11639	T11637	arg1Of	proteins,vitro
R9486	T11639	T11638	arg2Of	proteins,translated
R9487	T11642	T11640	arg2Of	assays,in
R9488	T11642	T11641	arg1Of	assays,coimmunoprecipitation
R9489	T11644	T11643	arg2Of	mapping,For
R9490	T11647	T11644	arg2Of	domains,mapping
R9491	T11647	T11645	arg1Of	domains,the
R9492	T11647	T11646	arg1Of	domains,interaction
R9493	T11649	T11650	arg1Of	we,used
R9494	T11650	T11643	arg1Of	used,For
R9495	T11650	T11648	arg1Of	used,","
R9496	T11650	T11655	arg1Of	used,:
R9497	T11654	T11650	arg2Of	mutants,used
R9498	T11654	T11651	arg1Of	mutants,the
R9499	T11654	T11652	arg1Of	mutants,following
R9500	T11654	T11653	arg1Of	mutants,Tat
R9501	T11657	T11656	arg1Of	T,Tat
R9502	T11657	T11658	arg1Of	T,","
R9503	T11657	T11664	arg1Of	T,","
R9504	T11661	T11660	arg2Of	"23,24",(
R9505	T11662	T11660	arg3Of	),(
R9506	T11663	T11658	arg2Of	A,","
R9507	T11663	T11659	arg1Of	A,N
R9508	T11663	T11660	arg1Of	A,(
R9509	T11664	T11671	arg1Of	",",","
R9510	T11668	T11667	arg2Of	"22,25,27",(
R9511	T11669	T11667	arg3Of	),(
R9512	T11670	T11664	arg2Of	A,","
R9513	T11670	T11665	arg1Of	A,Tat
R9514	T11670	T11666	arg1Of	A,C
R9515	T11670	T11667	arg1Of	A,(
R9516	T11675	T11674	arg2Of	"50,51",(
R9517	T11676	T11674	arg3Of	),(
R9518	T11677	T11672	arg1Of	A,Tat
R9519	T11677	T11673	arg1Of	A,K
R9520	T11677	T11674	arg1Of	A,(
R9521	T11677	T11678	arg1Of	A,and
R9522	T11678	T11671	arg2Of	and,","
R9523	T11680	T11679	arg1Of	R,Tat
R9524	T11680	T11681	arg1Of	R,(
R9525	T11682	T11681	arg2Of	49–57,(
R9526	T11683	T11681	arg3Of	),(
R9527	T11684	T11678	arg2Of	A,and
R9528	T11684	T11680	arg1Of	A,R
R9529	T11684	T11685	arg1Of	A,(
R9530	T11687	T11685	arg2Of	3A,(
R9531	T11687	T11686	arg1Of	3A,Figure
R9532	T11688	T11685	arg3Of	),(
R9533	T11689	T11690	arg1Of	IκB-α,coimmunoprecipitated
R9534	T11690	T11691	arg1Of	coimmunoprecipitated,with
R9535	T11690	T11695	arg1Of	coimmunoprecipitated,","
R9536	T11694	T11691	arg2Of	proteins,with
R9537	T11694	T11692	arg1Of	proteins,all
R9538	T11694	T11693	arg1Of	proteins,Tat
R9539	T11698	T11697	arg1Of	R,Tat
R9540	T11698	T11699	arg1Of	R,(
R9541	T11700	T11699	arg2Of	49–57,(
R9542	T11701	T11699	arg3Of	),(
R9543	T11702	T11690	arg2Of	A,coimmunoprecipitated
R9544	T11702	T11696	arg1Of	A,except
R9545	T11702	T11698	arg1Of	A,R
R9546	T11702	T11703	arg1Of	A,(
R9547	T11705	T11703	arg2Of	3B,(
R9548	T11705	T11704	arg1Of	3B,Figure
R9549	T11706	T11703	arg3Of	),(
R9550	T11708	T11707	arg1Of	results,These
R9551	T11708	T11709	arg1Of	results,indicated
R9552	T11713	T11711	arg1Of	domain,the
R9553	T11713	T11712	arg1Of	domain,arginine-rich
R9554	T11713	T11714	arg1Of	domain,of
R9555	T11713	T11716	arg1Of	domain,was
R9556	T11713	T11717	arg2Of	domain,involved
R9557	T11715	T11714	arg2Of	Tat,of
R9558	T11717	T11709	arg2Of	involved,indicated
R9559	T11717	T11710	arg1Of	involved,that
R9560	T11717	T11716	arg2Of	involved,was
R9561	T11717	T11718	arg1Of	involved,in
R9562	T11720	T11718	arg2Of	binding,in
R9563	T11720	T11719	arg1Of	binding,the
R9564	T11720	T11721	arg1Of	binding,to
R9565	T11720	T11723	arg1Of	binding,","
R9566	T11720	T11724	arg1Of	binding,which
R9567	T11720	T11725	arg1Of	binding,was
R9568	T11720	T11726	arg1Of	binding,consistent
R9569	T11722	T11721	arg2Of	IκB-α,to
R9570	T11726	T11725	arg2Of	consistent,was
R9571	T11726	T11727	arg1Of	consistent,with
R9572	T11729	T11727	arg2Of	observations,with
R9573	T11729	T11728	arg1Of	observations,previous
R9574	T11729	T11730	arg1Of	observations,(
R9575	T11731	T11730	arg2Of	"50,51",(
R9576	T11732	T11730	arg3Of	),(
R9577	T11735	T11736	arg1Of	Tat,associating
R9578	T11735	T11739	arg1Of	Tat,competed
R9579	T11736	T11737	arg1Of	associating,with
R9580	T11738	T11737	arg2Of	IκB-α,with
R9581	T11739	T11733	arg1Of	competed,Moreover
R9582	T11739	T11734	arg1Of	competed,","
R9583	T11739	T11757	arg1Of	competed,","
R9584	T11739	T11758	arg1Of	competed,while
R9585	T11741	T11739	arg2Of	binding,competed
R9586	T11741	T11740	arg1Of	binding,the
R9587	T11741	T11742	arg1Of	binding,of
R9588	T11741	T11744	arg1Of	binding,to
R9589	T11741	T11746	arg1Of	binding,in
R9590	T11743	T11742	arg2Of	IκB-α,of
R9591	T11745	T11744	arg2Of	p65,to
R9592	T11749	T11746	arg2Of	manner,in
R9593	T11749	T11747	arg1Of	manner,a
R9594	T11749	T11748	arg1Of	manner,dose-dependent
R9595	T11749	T11750	arg1Of	manner,(
R9596	T11752	T11750	arg2Of	3C,(
R9597	T11752	T11751	arg1Of	3C,Figure
R9598	T11752	T11753	arg1Of	3C,","
R9599	T11754	T11753	arg2Of	lanes,","
R9600	T11754	T11755	arg1Of	lanes,3–5
R9601	T11756	T11750	arg3Of	),(
R9602	T11760	T11759	arg1Of	R,Tat
R9603	T11760	T11761	arg1Of	R,(
R9604	T11762	T11761	arg2Of	49–57,(
R9605	T11763	T11761	arg3Of	),(
R9606	T11764	T11760	arg1Of	A,R
R9607	T11764	T11765	arg1Of	A,","
R9608	T11764	T11766	arg1Of	A,which
R9609	T11764	T11767	arg1Of	A,lacked
R9610	T11764	T11774	arg1Of	A,did
R9611	T11764	T11776	arg1Of	A,associate
R9612	T11764	T11780	arg1Of	A,did
R9613	T11764	T11782	arg1Of	A,compete
R9614	T11770	T11767	arg2Of	site,lacked
R9615	T11770	T11768	arg1Of	site,the
R9616	T11770	T11769	arg1Of	site,binding
R9617	T11770	T11771	arg1Of	site,for
R9618	T11772	T11771	arg2Of	IκB-α,for
R9619	T11776	T11774	arg2Of	associate,did
R9620	T11776	T11775	arg1Of	associate,not
R9621	T11776	T11777	arg1Of	associate,with
R9622	T11776	T11779	arg1Of	associate,and
R9623	T11778	T11777	arg2Of	IκB-α,with
R9624	T11779	T11758	arg2Of	and,while
R9625	T11779	T11773	arg1Of	and,","
R9626	T11782	T11779	arg2Of	compete,and
R9627	T11782	T11780	arg2Of	compete,did
R9628	T11782	T11781	arg1Of	compete,not
R9629	T11784	T11782	arg2Of	binding,compete
R9630	T11784	T11783	arg1Of	binding,the
R9631	T11784	T11785	arg1Of	binding,of
R9632	T11784	T11787	arg1Of	binding,to
R9633	T11786	T11785	arg2Of	IκB-α,of
R9634	T11788	T11787	arg2Of	p65,to
R9635	T11788	T11789	arg1Of	p65,(
R9636	T11791	T11789	arg2Of	3C,(
R9637	T11791	T11790	arg1Of	3C,Figure
R9638	T11791	T11792	arg1Of	3C,","
R9639	T11793	T11792	arg2Of	lanes,","
R9640	T11793	T11794	arg1Of	lanes,6–8
R9641	T11795	T11789	arg3Of	),(
R9642	T11798	T11799	arg1Of	we,evaluated
R9643	T11799	T11796	arg1Of	evaluated,Next
R9644	T11799	T11797	arg1Of	evaluated,","
R9645	T11801	T11802	arg1Of	Tat,counteracted
R9646	T11801	T11812	arg1Of	Tat,incubating
R9647	T11802	T11799	arg2Of	counteracted,evaluated
R9648	T11802	T11800	arg1Of	counteracted,whether
R9649	T11802	T11811	arg1Of	counteracted,by
R9650	T11805	T11802	arg2Of	inhibition,counteracted
R9651	T11805	T11803	arg1Of	inhibition,the
R9652	T11805	T11804	arg1Of	inhibition,IκB-α-mediated
R9653	T11805	T11806	arg1Of	inhibition,of
R9654	T11805	T11809	arg1Of	inhibition,to
R9655	T11808	T11806	arg2Of	binding,of
R9656	T11808	T11807	arg1Of	binding,p65
R9657	T11810	T11809	arg2Of	DNA,to
R9658	T11812	T11811	arg2Of	incubating,by
R9659	T11812	T11824	arg1Of	incubating,in
R9660	T11814	T11813	arg1Of	vitro,in
R9661	T11816	T11815	arg2Of	p65,translated
R9662	T11816	T11817	arg1Of	p65,and
R9663	T11817	T11812	arg2Of	and,incubating
R9664	T11817	T11814	arg1Of	and,vitro
R9665	T11817	T11820	arg1Of	and,with
R9666	T11819	T11817	arg2Of	proteins,and
R9667	T11819	T11818	arg1Of	proteins,IκB-α
R9668	T11823	T11820	arg2Of	probe,with
R9669	T11823	T11821	arg1Of	probe,the
R9670	T11823	T11822	arg1Of	probe,NF-κB
R9671	T11825	T11826	arg1Of	presence,or
R9672	T11826	T11824	arg2Of	or,in
R9673	T11826	T11828	arg1Of	or,of
R9674	T11827	T11826	arg2Of	absence,or
R9675	T11829	T11828	arg2Of	Tat,of
R9676	T11829	T11830	arg2Of	Tat,followed
R9677	T11832	T11830	arg1Of	EMSA,followed
R9678	T11832	T11831	arg2Of	EMSA,by
R9679	T11837	T11833	arg1Of	activity,The
R9680	T11837	T11834	arg1Of	activity,p65
R9681	T11837	T11835	arg1Of	activity,DNA
R9682	T11837	T11836	arg1Of	activity,binding
R9683	T11837	T11838	arg1Of	activity,was
R9684	T11837	T11839	arg2Of	activity,inhibited
R9685	T11837	T11843	arg2Of	activity,restored
R9686	T11839	T11842	arg1Of	inhibited,and
R9687	T11841	T11839	arg1Of	IκB-α,inhibited
R9688	T11841	T11840	arg2Of	IκB-α,by
R9689	T11842	T11838	arg2Of	and,was
R9690	T11843	T11842	arg2Of	restored,and
R9691	T11843	T11844	arg1Of	restored,in
R9692	T11843	T11848	arg1Of	restored,by
R9693	T11843	T11854	arg1Of	restored,by
R9694	T11847	T11844	arg2Of	manner,in
R9695	T11847	T11845	arg1Of	manner,a
R9696	T11847	T11846	arg1Of	manner,dose-dependent
R9697	T11848	T11852	arg1Of	by,and
R9698	T11850	T11848	arg2Of	Tat,by
R9699	T11850	T11849	arg1Of	Tat,wild-type
R9700	T11852	T11851	arg1Of	and,","
R9701	T11854	T11852	arg2Of	by,and
R9702	T11854	T11853	arg1Of	by,not
R9703	T11856	T11854	arg2Of	R,by
R9704	T11856	T11855	arg1Of	R,Tat
R9705	T11856	T11857	arg1Of	R,(
R9706	T11856	T11860	arg1Of	R,(
R9707	T11858	T11857	arg2Of	49–57,(
R9708	T11859	T11857	arg3Of	),(
R9709	T11862	T11860	arg2Of	3D,(
R9710	T11862	T11861	arg1Of	3D,Figure
R9711	T11863	T11860	arg3Of	),(
R9712	T11866	T11867	arg1Of	we,analysed
R9713	T11866	T11880	arg1Of	we,transfecting
R9714	T11867	T11864	arg1Of	analysed,Further
R9715	T11867	T11865	arg1Of	analysed,","
R9716	T11867	T11871	arg1Of	analysed,on
R9717	T11867	T11879	arg1Of	analysed,by
R9718	T11870	T11867	arg2Of	effect,analysed
R9719	T11870	T11868	arg1Of	effect,the
R9720	T11870	T11869	arg1Of	effect,Tat
R9721	T11874	T11871	arg2Of	repression,on
R9722	T11874	T11872	arg1Of	repression,the
R9723	T11874	T11873	arg1Of	repression,IκB-α
R9724	T11874	T11875	arg1Of	repression,of
R9725	T11878	T11875	arg2Of	activity,of
R9726	T11878	T11876	arg1Of	activity,p65
R9727	T11878	T11877	arg1Of	activity,transcriptional
R9728	T11880	T11879	arg2Of	transfecting,by
R9729	T11880	T11882	arg1Of	transfecting,with
R9730	T11880	T11886	arg1Of	transfecting,together
R9731	T11880	T11887	arg1Of	transfecting,with
R9732	T11880	T11894	arg1Of	transfecting,","
R9733	T11880	T11895	arg1Of	transfecting,in
R9734	T11881	T11880	arg2Of	p50−/−p65−/−MEFs,transfecting
R9735	T11885	T11882	arg2Of	reporter,with
R9736	T11885	T11883	arg1Of	reporter,the
R9737	T11885	T11884	arg1Of	reporter,NF-κB-Luc
R9738	T11889	T11887	arg2Of	vectors,with
R9739	T11889	T11888	arg1Of	vectors,expression
R9740	T11889	T11890	arg1Of	vectors,of
R9741	T11891	T11892	arg1Of	p65,and
R9742	T11892	T11890	arg2Of	and,of
R9743	T11893	T11892	arg2Of	IκB-α,and
R9744	T11896	T11897	arg1Of	presence,or
R9745	T11897	T11899	arg1Of	or,of
R9746	T11897	T11902	arg1Of	or,inhibited
R9747	T11898	T11897	arg2Of	absence,or
R9748	T11901	T11899	arg2Of	IκB-α,of
R9749	T11901	T11900	arg1Of	IκB-α,Tat.
R9750	T11902	T11895	arg2Of	inhibited,in
R9751	T11905	T11902	arg2Of	expression,inhibited
R9752	T11905	T11903	arg1Of	expression,the
R9753	T11905	T11904	arg1Of	expression,p65-dependent
R9754	T11905	T11906	arg1Of	expression,of
R9755	T11905	T11924	arg1Of	expression,by
R9756	T11906	T11922	arg1Of	of,and
R9757	T11909	T11906	arg2Of	gene,of
R9758	T11909	T11907	arg1Of	gene,the
R9759	T11909	T11908	arg1Of	gene,luciferase
R9760	T11909	T11910	arg1Of	gene,","
R9761	T11909	T11911	arg1Of	gene,which
R9762	T11909	T11912	arg1Of	gene,was
R9763	T11909	T11913	arg2Of	gene,restored
R9764	T11913	T11912	arg2Of	restored,was
R9765	T11913	T11914	arg1Of	restored,in
R9766	T11917	T11914	arg2Of	manner,in
R9767	T11917	T11915	arg1Of	manner,a
R9768	T11917	T11916	arg1Of	manner,dose-dependent
R9769	T11920	T11913	arg1Of	Tat,restored
R9770	T11920	T11918	arg2Of	Tat,by
R9771	T11920	T11919	arg1Of	Tat,wild-type
R9772	T11922	T11921	arg1Of	and,","
R9773	T11924	T11922	arg2Of	by,and
R9774	T11924	T11923	arg1Of	by,not
R9775	T11926	T11925	arg1Of	R,Tat
R9776	T11926	T11927	arg1Of	R,(
R9777	T11928	T11927	arg2Of	49–57,(
R9778	T11929	T11927	arg3Of	),(
R9779	T11930	T11924	arg2Of	A,by
R9780	T11930	T11926	arg1Of	A,R
R9781	T11930	T11931	arg1Of	A,(
R9782	T11933	T11931	arg2Of	3E,(
R9783	T11933	T11932	arg1Of	3E,Figure
R9784	T11934	T11931	arg3Of	),(
R9785	T11937	T11936	arg1Of	results,these
R9786	T11937	T11938	arg1Of	results,indicated
R9787	T11938	T11935	arg1Of	indicated,Altogether
R9788	T11940	T11941	arg1Of	Tat,counteracts
R9789	T11940	T11953	arg1Of	Tat,associating
R9790	T11940	T11957	arg1Of	Tat,competing
R9791	T11941	T11938	arg2Of	counteracts,indicated
R9792	T11941	T11939	arg1Of	counteracts,that
R9793	T11941	T11952	arg1Of	counteracts,by
R9794	T11944	T11941	arg2Of	repression,counteracts
R9795	T11944	T11942	arg1Of	repression,the
R9796	T11944	T11943	arg1Of	repression,IκB-α
R9797	T11944	T11945	arg1Of	repression,of
R9798	T11948	T11949	arg1Of	DNA-binding,and
R9799	T11950	T11949	arg2Of	transcriptional,and
R9800	T11951	T11945	arg2Of	activity,of
R9801	T11951	T11946	arg1Of	activity,the
R9802	T11951	T11947	arg1Of	activity,p65
R9803	T11951	T11948	arg1Of	activity,DNA-binding
R9804	T11951	T11950	arg1Of	activity,transcriptional
R9805	T11953	T11954	arg1Of	associating,with
R9806	T11953	T11956	arg1Of	associating,and
R9807	T11955	T11954	arg2Of	IκB-α,with
R9808	T11956	T11952	arg2Of	and,by
R9809	T11957	T11956	arg2Of	competing,and
R9810	T11960	T11957	arg2Of	binding,competing
R9811	T11960	T11958	arg1Of	binding,the
R9812	T11960	T11959	arg1Of	binding,repressor
R9813	T11960	T11961	arg1Of	binding,to
R9814	T11962	T11961	arg2Of	p65,to
R20033	T25214	T25215	arg1Of	Tat,relieves
R20034	T25214	T25280	arg1Of	Tat,using
R20035	T25215	T25280	modOf	relieves,using
R20036	T25216	T25217	arg1Of	p65,from
R20037	T25216	T25226	arg1Of	p65,of
R20038	T25216	T25263	arg1Of	p65,","
R20039	T25222	T25221	arg2Of	A,(
R20040	T25223	T25221	arg3Of	),(
R20041	T25225	T25217	arg2Of	representation,from
R20042	T25225	T25218	arg1Of	representation,the
R20043	T25225	T25219	arg1Of	representation,IκB-α
R20044	T25225	T25220	arg1Of	representation,inhibition.
R20045	T25225	T25221	arg1Of	representation,(
R20046	T25225	T25224	arg1Of	representation,Schematic
R20047	T25227	T25228	arg1Of	wild-type,and
R20048	T25229	T25228	arg2Of	mutant,and
R20049	T25231	T25227	arg1Of	proteins.,wild-type
R20050	T25231	T25229	arg1Of	proteins.,mutant
R20051	T25231	T25230	arg1Of	proteins.,Tat
R20052	T25231	T25232	arg1Of	proteins.,(
R20053	T25231	T25239	modOf	proteins.,was
R20054	T25231	T25243	arg1Of	proteins.,or
R20055	T25233	T25232	arg2Of	B,(
R20056	T25234	T25232	arg3Of	),(
R20057	T25235	T25236	arg1Of	HA-IκB-α,(
R20058	T25235	T25239	arg1Of	HA-IκB-α,was
R20059	T25235	T25240	arg2Of	HA-IκB-α,incubated
R20060	T25237	T25236	arg2Of	5 µl,(
R20061	T25238	T25236	arg3Of	),(
R20062	T25240	T25239	arg2Of	incubated,was
R20063	T25240	T25241	arg1Of	incubated,in
R20064	T25242	T25241	arg2Of	presence,in
R20065	T25243	T25226	arg2Of	or,of
R20066	T25244	T25245	arg1Of	absence,of
R20067	T25244	T25256	arg1Of	absence,","
R20068	T25246	T25245	arg2Of	FLAG-Tat,of
R20069	T25246	T25247	arg1Of	FLAG-Tat,","
R20070	T25246	T25250	arg1Of	FLAG-Tat,","
R20071	T25249	T25247	arg2Of	T,","
R20072	T25249	T25248	arg1Of	T,FLAG-Tat
R20073	T25253	T25252	arg2Of	"23,24",(
R20074	T25254	T25252	arg3Of	),(
R20075	T25255	T25250	arg2Of	A,","
R20076	T25255	T25251	arg1Of	A,N
R20077	T25255	T25252	arg1Of	A,(
R20078	T25256	T25243	arg2Of	",",or
R20079	T25260	T25259	arg2Of	"50,51",(
R20080	T25261	T25259	arg3Of	),(
R20081	T25262	T25256	arg2Of	A,","
R20082	T25262	T25257	arg1Of	A,FLAG-Tat
R20083	T25262	T25258	arg1Of	A,K
R20084	T25262	T25259	arg1Of	A,(
R20085	T25263	T25270	arg1Of	",",or
R20086	T25265	T25264	arg1Of	R,FLAG-Tat
R20087	T25265	T25266	arg1Of	R,(
R20088	T25267	T25266	arg2Of	49–57,(
R20089	T25268	T25266	arg3Of	),(
R20090	T25269	T25263	arg2Of	A,","
R20091	T25269	T25265	arg1Of	A,R
R20092	T25270	T25215	arg2Of	or,relieves
R20093	T25274	T25273	arg2Of	"22,25,27",(
R20094	T25275	T25273	arg3Of	),(
R20095	T25276	T25270	arg2Of	A,or
R20096	T25276	T25271	arg1Of	A,FLAG-Tat
R20097	T25276	T25272	arg1Of	A,C
R20098	T25276	T25273	arg1Of	A,(
R20099	T25276	T25277	arg1Of	A,(
R20100	T25278	T25277	arg2Of	10 µl,(
R20101	T25279	T25277	arg3Of	),(
R20102	T25284	T25280	arg2Of	proteins,using
R20103	T25284	T25281	arg1Of	proteins,in
R20104	T25284	T25282	arg1Of	proteins,vitro
R20105	T25284	T25283	arg2Of	proteins,translated
R20106	T25285	T25286	arg1Of	Wild-type,and
R20107	T25287	T25286	arg2Of	mutant,and
R20108	T25289	T25285	arg1Of	proteins,Wild-type
R20109	T25289	T25287	arg1Of	proteins,mutant
R20110	T25289	T25288	arg1Of	proteins,Tat
R20111	T25289	T25290	arg1Of	proteins,were
R20112	T25289	T25291	arg2Of	proteins,immunoprecipitated
R20113	T25291	T25290	arg2Of	immunoprecipitated,were
R20114	T25291	T25292	arg1Of	immunoprecipitated,with
R20115	T25291	T25295	arg1Of	immunoprecipitated,;
R20116	T25294	T25292	arg2Of	antibody,with
R20117	T25294	T25293	arg1Of	antibody,anti-FLAG
R20118	T25296	T25297	arg1Of	immunocomplexes,were
R20119	T25296	T25298	arg2Of	immunocomplexes,separated
R20120	T25296	T25304	arg2Of	immunocomplexes,analysed
R20121	T25298	T25303	arg1Of	separated,and
R20122	T25300	T25301	arg1Of	12,%
R20123	T25302	T25298	arg1Of	SDS–PAGE,separated
R20124	T25302	T25299	arg2Of	SDS–PAGE,by
R20125	T25302	T25300	arg1Of	SDS–PAGE,12
R20126	T25303	T25295	arg2Of	and,;
R20127	T25303	T25297	arg2Of	and,were
R20128	T25303	T25308	arg1Of	and,with
R20129	T25304	T25303	arg2Of	analysed,and
R20130	T25307	T25304	arg1Of	blotting,analysed
R20131	T25307	T25305	arg2Of	blotting,by
R20132	T25307	T25306	arg1Of	blotting,western
R20133	T25309	T25310	arg1Of	anti-HA,and
R20134	T25311	T25310	arg2Of	anti-FLAG,and
R20135	T25312	T25308	arg2Of	antibodies.,with
R20136	T25312	T25309	arg1Of	antibodies.,anti-HA
R20137	T25312	T25311	arg1Of	antibodies.,anti-FLAG
R20138	T25312	T25313	arg1Of	antibodies.,(
R20139	T25312	T25321	modOf	antibodies.,was
R20140	T25314	T25313	arg2Of	C,(
R20141	T25315	T25313	arg3Of	),(
R20142	T25317	T25316	arg1Of	p65,35S-methionine-labeled
R20143	T25317	T25318	arg1Of	p65,(
R20144	T25317	T25321	arg1Of	p65,was
R20145	T25317	T25322	arg2Of	p65,incubated
R20146	T25319	T25318	arg2Of	5 µl,(
R20147	T25320	T25318	arg3Of	),(
R20148	T25322	T25321	arg2Of	incubated,was
R20149	T25322	T25323	arg1Of	incubated,with
R20150	T25325	T25324	arg1Of	vitro,in
R20151	T25327	T25323	arg2Of	HA-IκB-α,with
R20152	T25327	T25325	arg1Of	HA-IκB-α,vitro
R20153	T25327	T25326	arg2Of	HA-IκB-α,translated
R20154	T25327	T25328	arg1Of	HA-IκB-α,(
R20155	T25327	T25331	arg1Of	HA-IκB-α,in
R20156	T25327	T25335	arg1Of	HA-IκB-α,of
R20157	T25329	T25328	arg2Of	5 µl,(
R20158	T25330	T25328	arg3Of	),(
R20159	T25332	T25333	arg1Of	presence,or
R20160	T25333	T25331	arg2Of	or,in
R20161	T25334	T25333	arg2Of	absence,or
R20162	T25336	T25337	arg1Of	FLAG-Tat,or
R20163	T25339	T25337	arg2Of	R,or
R20164	T25339	T25338	arg1Of	R,FLAG-Tat
R20165	T25339	T25340	arg1Of	R,(
R20166	T25341	T25340	arg2Of	49–57,(
R20167	T25342	T25340	arg3Of	),(
R20168	T25343	T25335	arg2Of	A,of
R20169	T25343	T25336	arg1Of	A,FLAG-Tat
R20170	T25343	T25339	arg1Of	A,R
R20171	T25343	T25344	arg1Of	A,(
R20172	T25345	T25346	arg1Of	5,","
R20173	T25346	T25348	arg1Of	",",or
R20174	T25347	T25346	arg2Of	10,","
R20175	T25348	T25344	arg2Of	or,(
R20176	T25349	T25348	arg2Of	20 µl,or
R20177	T25350	T25344	arg3Of	),(
R20178	T25351	T25352	arg1Of	HA-IκB-α,was
R20179	T25351	T25353	arg2Of	HA-IκB-α,immunoprecipitated
R20180	T25353	T25352	arg2Of	immunoprecipitated,was
R20181	T25353	T25354	arg1Of	immunoprecipitated,with
R20182	T25353	T25357	arg1Of	immunoprecipitated,;
R20183	T25356	T25354	arg2Of	antibody,with
R20184	T25356	T25355	arg1Of	antibody,anti-HA
R20185	T25358	T25359	arg1Of	immunocomplexes,were
R20186	T25358	T25360	arg2Of	immunocomplexes,separated
R20187	T25358	T25366	arg2Of	immunocomplexes,analysed
R20188	T25360	T25365	arg1Of	separated,and
R20189	T25362	T25363	arg1Of	12,%
R20190	T25364	T25360	arg1Of	SDS–PAGE,separated
R20191	T25364	T25361	arg2Of	SDS–PAGE,by
R20192	T25364	T25362	arg1Of	SDS–PAGE,12
R20193	T25365	T25357	arg2Of	and,;
R20194	T25365	T25359	arg2Of	and,were
R20195	T25366	T25365	arg2Of	analysed,and
R20196	T25369	T25368	arg1Of	blotting,western
R20197	T25369	T25370	arg1Of	blotting,with
R20198	T25369	T25376	arg1Of	blotting,or
R20199	T25371	T25372	arg1Of	anti-HA,and
R20200	T25373	T25372	arg2Of	anti-FLAG,and
R20201	T25374	T25370	arg2Of	antibodies,with
R20202	T25374	T25371	arg1Of	antibodies,anti-HA
R20203	T25374	T25373	arg1Of	antibodies,anti-FLAG
R20204	T25376	T25366	arg1Of	or,analysed
R20205	T25376	T25367	arg2Of	or,by
R20206	T25376	T25375	arg1Of	or,","
R20207	T25377	T25376	arg2Of	autoradiography,or
R20208	T25377	T25378	arg1Of	autoradiography,(
R20209	T25379	T25378	arg2Of	35S-Met-p65,(
R20210	T25380	T25378	arg3Of	),(
R20211	T25382	T25381	arg1Of	values,Densitometry
R20212	T25382	T25383	arg1Of	values,(
R20213	T25382	T25386	arg1Of	values,of
R20214	T25382	T25389	arg1Of	values,were
R20215	T25382	T25390	arg2Of	values,expressed
R20216	T25384	T25383	arg2Of	D,(
R20217	T25385	T25383	arg3Of	),(
R20218	T25388	T25386	arg2Of	bands,of
R20219	T25388	T25387	arg1Of	bands,the
R20220	T25390	T25389	arg2Of	expressed,were
R20221	T25390	T25391	arg1Of	expressed,as
R20222	T25393	T25391	arg2Of	increase,as
R20223	T25393	T25392	arg1Of	increase,fold
R20224	T25393	T25394	arg1Of	increase,above
R20225	T25393	T25401	arg1Of	increase,.
R20226	T25396	T25394	arg2Of	control,above
R20227	T25396	T25395	arg1Of	control,the
R20228	T25396	T25397	arg1Of	control,(
R20229	T25398	T25397	arg2Of	lane,(
R20230	T25398	T25399	arg1Of	lane,1
R20231	T25400	T25397	arg3Of	),(
R20232	T25402	T25401	arg2Of	(,.
R20233	T25403	T25401	arg3Of	D,.
R20234	T25408	T25404	arg1Of	ding act,) T
R20235	T25408	T25405	arg1Of	ding act,he
R20236	T25408	T25406	arg1Of	ding act,p65
R20237	T25408	T25407	arg1Of	ding act,DNA bin
R20238	T25408	T25409	arg1Of	ding act,ivi
R20239	T25408	T25410	arg2Of	ding act,ty was a
R20240	T25410	T25389	modOf	ty was a,were
R20241	T25410	T25409	arg2Of	ty was a,ivi
R20242	T25412	T25410	arg1Of	lyse,ty was a
R20243	T25412	T25411	arg2Of	lyse,na
R20244	T25412	T25413	arg1Of	lyse,d by
R20245	T25415	T25414	arg1Of	SA us,EM
R20246	T25417	T25413	arg2Of	ro trans,d by
R20247	T25417	T25415	arg1Of	ro trans,SA us
R20248	T25417	T25416	arg2Of	ro trans,ing in vit
R20249	T25420	T25418	arg1Of	oligonucleotide,32P-labeled-NF-κB
R20250	T25420	T25419	arg1Of	oligonucleotide,double-stranded
R20251	T25420	T25421	arg1Of	oligonucleotide,was
R20252	T25420	T25422	arg2Of	oligonucleotide,incubated
R20253	T25422	T25421	arg2Of	incubated,was
R20254	T25422	T25423	arg1Of	incubated,with
R20255	T25422	T25451	arg1Of	incubated,;
R20256	T25424	T25423	arg2Of	p65,with
R20257	T25424	T25425	arg1Of	p65,(
R20258	T25424	T25428	arg1Of	p65,in
R20259	T25424	T25432	arg1Of	p65,of
R20260	T25426	T25425	arg2Of	0.5 µl,(
R20261	T25427	T25425	arg3Of	),(
R20262	T25429	T25430	arg1Of	presence,or
R20263	T25430	T25428	arg2Of	or,in
R20264	T25431	T25430	arg2Of	absence,or
R20265	T25433	T25434	arg1Of	HA-IκB-α,(
R20266	T25433	T25437	arg1Of	HA-IκB-α,","
R20267	T25435	T25434	arg2Of	1 µl,(
R20268	T25436	T25434	arg3Of	),(
R20269	T25437	T25439	arg1Of	",",or
R20270	T25438	T25437	arg2Of	FLAG-Tat,","
R20271	T25439	T25432	arg2Of	or,of
R20272	T25441	T25440	arg1Of	R,FLAG-Tat
R20273	T25441	T25442	arg1Of	R,(
R20274	T25443	T25442	arg2Of	49–57,(
R20275	T25444	T25442	arg3Of	),(
R20276	T25445	T25439	arg2Of	A,or
R20277	T25445	T25441	arg1Of	A,R
R20278	T25445	T25446	arg1Of	A,(
R20279	T25447	T25448	arg1Of	5,and
R20280	T25448	T25446	arg2Of	and,(
R20281	T25449	T25448	arg2Of	10 µl,and
R20282	T25450	T25446	arg3Of	),(
R20283	T25452	T25453	arg1Of	competition,of
R20284	T25452	T25456	arg1Of	competition,was
R20285	T25452	T25457	arg2Of	competition,performed
R20286	T25455	T25453	arg2Of	binding,of
R20287	T25455	T25454	arg1Of	binding,DNA
R20288	T25457	T25451	arg2Of	performed,;
R20289	T25457	T25456	arg2Of	performed,was
R20290	T25457	T25458	arg1Of	performed,with
R20291	T25461	T25460	arg1Of	to,up
R20292	T25464	T25458	arg2Of	excess,with
R20293	T25464	T25459	arg1Of	excess,10 -
R20294	T25464	T25461	arg1Of	excess,to
R20295	T25464	T25462	arg1Of	excess,100-fold
R20296	T25464	T25463	arg1Of	excess,molar
R20297	T25464	T25465	arg1Of	excess,of
R20298	T25469	T25465	arg2Of	oligonucleotide,of
R20299	T25469	T25466	arg1Of	oligonucleotide,unlabeled
R20300	T25469	T25467	arg1Of	oligonucleotide,NF-κB
R20301	T25469	T25468	arg1Of	oligonucleotide,double-stranded
R20302	T25471	T25470	arg1Of	complexes,DNA/protein
R20303	T25471	T25472	arg1Of	complexes,were
R20304	T25471	T25473	arg2Of	complexes,run
R20305	T25471	T25479	arg2Of	complexes,analysed
R20306	T25473	T25474	arg1Of	run,on
R20307	T25473	T25478	arg1Of	run,and
R20308	T25475	T25476	arg1Of	6,%
R20309	T25477	T25474	arg2Of	PAGE–TBE,on
R20310	T25477	T25475	arg1Of	PAGE–TBE,6
R20311	T25478	T25472	arg2Of	and,were
R20312	T25479	T25478	arg2Of	analysed,and
R20313	T25481	T25479	arg1Of	autoradiography.,analysed
R20314	T25481	T25480	arg2Of	autoradiography.,by
R20315	T25481	T25482	arg1Of	autoradiography.,(
R20316	T25481	T25490	modOf	autoradiography.,were
R20317	T25483	T25482	arg2Of	E,(
R20318	T25484	T25482	arg3Of	),(
R20319	T25485	T25486	arg1Of	p50−/−p65−/−MEFs,(
R20320	T25485	T25490	arg1Of	p50−/−p65−/−MEFs,were
R20321	T25485	T25491	arg2Of	p50−/−p65−/−MEFs,transfected
R20322	T25488	T25486	arg2Of	cells,(
R20323	T25488	T25487	arg1Of	cells,3 × 105
R20324	T25489	T25486	arg3Of	),(
R20325	T25491	T25490	arg2Of	transfected,were
R20326	T25491	T25492	arg1Of	transfected,with
R20327	T25493	T25494	arg1Of	pκBLuc,(
R20328	T25493	T25497	arg1Of	pκBLuc,and
R20329	T25495	T25494	arg2Of	0.5 µg,(
R20330	T25496	T25494	arg3Of	),(
R20331	T25497	T25492	arg2Of	and,with
R20332	T25498	T25499	arg1Of	pSV-β-Gal,(
R20333	T25498	T25502	arg1Of	pSV-β-Gal,with
R20334	T25498	T25504	arg1Of	pSV-β-Gal,without
R20335	T25500	T25499	arg2Of	0.1 µg,(
R20336	T25501	T25499	arg3Of	),(
R20337	T25502	T25503	arg1Of	with,or
R20338	T25504	T25503	arg2Of	without,or
R20339	T25505	T25506	arg1Of	pRc/CMV-p65,(
R20340	T25505	T25509	arg1Of	pRc/CMV-p65,","
R20341	T25507	T25506	arg2Of	0.5 µg,(
R20342	T25508	T25506	arg3Of	),(
R20343	T25509	T25514	arg1Of	",",","
R20344	T25510	T25509	arg2Of	pCMV4-HA-IκB-α,","
R20345	T25510	T25511	arg1Of	pCMV4-HA-IκB-α,(
R20346	T25512	T25511	arg2Of	0.5 µg,(
R20347	T25513	T25511	arg3Of	),(
R20348	T25514	T25516	arg1Of	",",or
R20349	T25515	T25514	arg2Of	p3xFLAG-Tat,","
R20350	T25516	T25502	arg2Of	or,with
R20351	T25516	T25504	arg2Of	or,without
R20352	T25518	T25516	arg2Of	R,or
R20353	T25518	T25517	arg1Of	R,p3xFLAG-Tat
R20354	T25518	T25519	arg1Of	R,(
R20355	T25520	T25519	arg2Of	49–57,(
R20356	T25521	T25519	arg3Of	),(
R20357	T25522	T25497	arg2Of	A,and
R20358	T25522	T25498	arg1Of	A,pSV-β-Gal
R20359	T25522	T25523	arg1Of	A,(
R20360	T25524	T25525	arg1Of	0.5,","
R20361	T25525	T25527	arg1Of	",",and
R20362	T25526	T25525	arg2Of	1,","
R20363	T25527	T25523	arg2Of	and,(
R20364	T25528	T25527	arg2Of	2 µg,and
R20365	T25529	T25523	arg3Of	),(
R20366	T25532	T25530	arg1Of	activity,The
R20367	T25532	T25531	arg1Of	activity,luciferase
R20368	T25532	T25533	arg1Of	activity,was
R20369	T25532	T25534	arg2Of	activity,measured
R20370	T25532	T25541	arg2Of	activity,normalized
R20371	T25534	T25535	arg1Of	measured,in
R20372	T25534	T25540	arg1Of	measured,and
R20373	T25537	T25535	arg2Of	extracts,in
R20374	T25537	T25536	arg1Of	extracts,cell
R20375	T25537	T25539	arg1Of	extracts,post-transfection
R20376	T25539	T25538	arg1Of	post-transfection,48-h
R20377	T25540	T25533	arg2Of	and,was
R20378	T25541	T25540	arg2Of	normalized,and
R20379	T25541	T25542	arg1Of	normalized,to
R20380	T25544	T25542	arg2Of	activity,to
R20381	T25544	T25543	arg1Of	activity,β-galactosidase
R20382	T25546	T25545	arg1Of	activation,Fold
R20383	T25546	T25547	arg1Of	activation,was
R20384	T25546	T25548	arg2Of	activation,calculated
R20385	T25548	T25547	arg2Of	calculated,was
R20386	T25548	T25550	arg1Of	calculated,to
R20387	T25550	T25549	arg1Of	to,relative
R20388	T25551	T25550	arg2Of	transfection,to
R20389	T25551	T25552	arg1Of	transfection,of
R20390	T25555	T25552	arg2Of	plasmid,of
R20391	T25555	T25553	arg1Of	plasmid,the
R20392	T25555	T25554	arg1Of	plasmid,pκBLuc
R20393	T25555	T25556	arg1Of	plasmid,alone
R20394	T25557	T25558	arg1Of	Values,(
R20395	T25557	T25563	arg1Of	Values,are
R20396	T25557	T25564	arg2Of	Values,shown
R20397	T25559	T25558	arg2Of	mean ± SE,(
R20398	T25559	T25560	arg1Of	mean ± SE,","
R20399	T25561	T25560	arg2Of	n = 3,","
R20400	T25562	T25558	arg3Of	),(
R20401	T25564	T25563	arg2Of	shown,are
R20402	T25566	T25565	arg2Of	control,As
R20403	T25566	T25567	arg1Of	control,of
R20404	T25569	T25567	arg2Of	expression,of
R20405	T25569	T25568	arg1Of	expression,protein
R20406	T25571	T25572	arg1Of	aliquots,of
R20407	T25571	T25578	arg1Of	aliquots,were
R20408	T25571	T25579	arg2Of	aliquots,analysed
R20409	T25574	T25572	arg2Of	extracts,of
R20410	T25574	T25573	arg1Of	extracts,cell
R20411	T25574	T25575	arg1Of	extracts,(
R20412	T25576	T25575	arg2Of	20 µg,(
R20413	T25577	T25575	arg3Of	),(
R20414	T25579	T25565	arg1Of	analysed,As
R20415	T25579	T25570	arg1Of	analysed,","
R20416	T25579	T25578	arg2Of	analysed,were
R20417	T25582	T25579	arg1Of	blotting,analysed
R20418	T25582	T25580	arg2Of	blotting,by
R20419	T25582	T25581	arg1Of	blotting,western
R20420	T25582	T25583	arg1Of	blotting,with
R20421	T25584	T25585	arg1Of	anti-p65,","
R20422	T25585	T25587	arg1Of	",",","
R20423	T25586	T25585	arg2Of	anti-HA,","
R20424	T25587	T25589	arg1Of	",",and
R20425	T25588	T25587	arg2Of	anti-FLAG,","
R20426	T25590	T25589	arg2Of	anti-γ-tubulin,and
R20427	T25591	T25583	arg2Of	antibodies,with
R20428	T25591	T25584	arg1Of	antibodies,anti-p65
R20429	T25591	T25586	arg1Of	antibodies,anti-HA
R20430	T25591	T25588	arg1Of	antibodies,anti-FLAG
R20431	T25591	T25590	arg1Of	antibodies,anti-γ-tubulin

bionlp-st-ge-2016-coref

Id	Subject	Object	Predicate	Lexical cue
T11963	57-70	Anaphor	denotes	the repressor
T11964	20-25	Antecedent	denotes	IκB-α
T11965	3932-3945	Anaphor	denotes	the repressor
T11966	3912-3917	Antecedent	denotes	IκB-α
R9815	T11963	T11964	boundBy	the repressor,IκB-α
R9816	T11965	T11966	boundBy	the repressor,IκB-α

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T12077	0-3	NNP	denotes	Tat
T12078	4-15	VBZ	denotes	counteracts
T12079	16-19	DT	denotes	the
T12080	20-25	JJ	denotes	IκB-α
T12081	26-36	NN	denotes	inhibition
T12082	37-39	IN	denotes	of
T12083	40-43	CD	denotes	p65
T12084	44-46	IN	denotes	by
T12085	47-56	VBG	denotes	competing
T12086	57-60	DT	denotes	the
T12087	61-70	NN	denotes	repressor
T12088	71-78	JJ	denotes	binding
T12089	79-81	PRP	denotes	We
T12090	82-89	RB	denotes	further
T12091	90-102	VBD	denotes	investigated
T12092	103-106	DT	denotes	the
T12093	107-115	JJ	denotes	physical
T12094	116-127	NN	denotes	interaction
T12095	128-130	IN	denotes	of
T12096	131-134	NNP	denotes	Tat
T12097	135-139	IN	denotes	with
T12098	140-145	NNP	denotes	IκB-α
T12099	146-151	VBG	denotes	using
T12100	152-154	IN	denotes	in
T12101	155-160	NN	denotes	vitro
T12102	161-171	VBN	denotes	translated
T12103	172-180	NNS	denotes	proteins
T12104	181-183	IN	denotes	in
T12105	184-205	NN	denotes	coimmunoprecipitation
T12106	206-212	NNS	denotes	assays
T12107	212-213	.	denotes	.
T12108	214-217	IN	denotes	For
T12109	218-225	VBG	denotes	mapping
T12110	226-229	DT	denotes	the
T12111	230-241	NN	denotes	interaction
T12112	242-249	NNS	denotes	domains
T12113	249-250	,	denotes	,
T12114	251-253	PRP	denotes	we
T12115	254-258	VBD	denotes	used
T12116	259-262	DT	denotes	the
T12117	263-272	JJ	denotes	following
T12118	273-276	NNP	denotes	Tat
T12119	277-284	NNS	denotes	mutants
T12120	284-285	:	denotes	:
T12121	286-289	NNP	denotes	Tat
T12122	290-291	NNP	denotes	T
T12123	291-292	,	denotes	,
T12124	292-293	NNP	denotes	N
T12125	293-294	-LRB-	denotes	(
T12126	294-299	CD	denotes	23,24
T12127	299-300	-RRB-	denotes	)
T12128	300-301	DT	denotes	A
T12129	301-302	,	denotes	,
T12130	303-306	NNP	denotes	Tat
T12131	307-308	NNP	denotes	C
T12132	308-309	-LRB-	denotes	(
T12133	309-317	CD	denotes	22,25,27
T12134	317-318	-RRB-	denotes	)
T12135	318-319	DT	denotes	A
T12136	319-320	,	denotes	,
T12137	321-324	NNP	denotes	Tat
T12138	325-326	NNP	denotes	K
T12139	326-327	-LRB-	denotes	(
T12140	327-332	CD	denotes	50,51
T12141	332-333	-RRB-	denotes	)
T12142	333-334	DT	denotes	A
T12143	335-338	CC	denotes	and
T12144	339-342	NNP	denotes	Tat
T12145	343-344	NNP	denotes	R
T12146	344-345	-LRB-	denotes	(
T12147	345-347	CD	denotes	49
T12148	348-350	CD	denotes	57
T12149	350-351	-RRB-	denotes	)
T12150	351-352	DT	denotes	A
T12151	353-354	-LRB-	denotes	(
T12152	354-360	NN	denotes	Figure
T12153	361-363	NN	denotes	3A
T12154	363-364	-RRB-	denotes	)
T12155	364-365	.	denotes	.
T12156	366-371	JJ	denotes	IκB-α
T12157	372-392	VBN	denotes	coimmunoprecipitated
T12158	393-397	IN	denotes	with
T12159	398-401	DT	denotes	all
T12160	402-405	NNP	denotes	Tat
T12161	406-414	NNS	denotes	proteins
T12162	414-415	,	denotes	,
T12163	416-422	IN	denotes	except
T12164	423-426	NNP	denotes	Tat
T12165	427-428	NNP	denotes	R
T12166	428-429	-LRB-	denotes	(
T12167	429-431	CD	denotes	49
T12168	432-434	CD	denotes	57
T12169	434-435	-RRB-	denotes	)
T12170	435-436	DT	denotes	A
T12171	437-438	-LRB-	denotes	(
T12172	438-444	NN	denotes	Figure
T12173	445-447	NN	denotes	3B
T12174	447-448	-RRB-	denotes	)
T12175	448-449	.	denotes	.
T12176	450-455	DT	denotes	These
T12177	456-463	NNS	denotes	results
T12178	464-473	VBD	denotes	indicated
T12179	474-478	IN	denotes	that
T12180	479-482	DT	denotes	the
T12181	483-496	JJ	denotes	arginine-rich
T12182	497-503	NN	denotes	domain
T12183	504-506	IN	denotes	of
T12184	507-510	NNP	denotes	Tat
T12185	511-514	VBD	denotes	was
T12186	515-523	VBN	denotes	involved
T12187	524-526	IN	denotes	in
T12188	527-530	DT	denotes	the
T12189	531-538	JJ	denotes	binding
T12190	539-541	TO	denotes	to
T12191	542-547	NNP	denotes	IκB-α
T12192	547-548	,	denotes	,
T12193	549-554	WDT	denotes	which
T12194	555-558	VBD	denotes	was
T12195	559-569	JJ	denotes	consistent
T12196	570-574	IN	denotes	with
T12197	575-583	JJ	denotes	previous
T12198	584-596	NNS	denotes	observations
T12199	597-598	-LRB-	denotes	(
T12200	598-603	CD	denotes	50,51
T12201	603-604	-RRB-	denotes	)
T12202	604-605	.	denotes	.
T12203	606-614	RB	denotes	Moreover
T12204	614-615	,	denotes	,
T12205	616-619	NNP	denotes	Tat
T12206	620-631	VBG	denotes	associating
T12207	632-636	IN	denotes	with
T12208	637-642	JJ	denotes	IκB-α
T12209	643-651	VBN	denotes	competed
T12210	652-655	DT	denotes	the
T12211	656-663	JJ	denotes	binding
T12212	664-666	IN	denotes	of
T12213	667-672	NNP	denotes	IκB-α
T12214	673-675	TO	denotes	to
T12215	676-679	VB	denotes	p65
T12216	680-682	IN	denotes	in
T12217	683-684	DT	denotes	a
T12218	685-699	JJ	denotes	dose-dependent
T12219	700-706	NN	denotes	manner
T12220	707-708	-LRB-	denotes	(
T12221	708-714	NN	denotes	Figure
T12222	715-717	NN	denotes	3C
T12223	717-718	,	denotes	,
T12224	719-724	NNS	denotes	lanes
T12225	725-726	CD	denotes	3
T12226	727-728	LS	denotes	5
T12227	728-729	-RRB-	denotes	)
T12228	729-730	,	denotes	,
T12229	731-736	IN	denotes	while
T12230	737-740	NNP	denotes	Tat
T12231	741-742	NNP	denotes	R
T12232	742-743	-LRB-	denotes	(
T12233	743-745	CD	denotes	49
T12234	746-748	CD	denotes	57
T12235	748-749	-RRB-	denotes	)
T12236	749-750	DT	denotes	A
T12237	750-751	,	denotes	,
T12238	752-757	WDT	denotes	which
T12239	758-764	VBD	denotes	lacked
T12240	765-768	DT	denotes	the
T12241	769-776	JJ	denotes	binding
T12242	777-781	NN	denotes	site
T12243	782-785	IN	denotes	for
T12244	786-791	NNP	denotes	IκB-α
T12245	791-792	,	denotes	,
T12246	793-796	VBD	denotes	did
T12247	797-800	RB	denotes	not
T12248	801-810	JJ	denotes	associate
T12249	811-815	IN	denotes	with
T12250	816-821	NNP	denotes	IκB-α
T12251	822-825	CC	denotes	and
T12252	826-829	VBD	denotes	did
T12253	830-833	RB	denotes	not
T12254	834-841	VB	denotes	compete
T12255	842-845	DT	denotes	the
T12256	846-853	JJ	denotes	binding
T12257	854-856	IN	denotes	of
T12258	857-862	NNP	denotes	IκB-α
T12259	863-865	TO	denotes	to
T12260	866-869	VB	denotes	p65
T12261	870-871	-LRB-	denotes	(
T12262	871-877	NN	denotes	Figure
T12263	878-880	NN	denotes	3C
T12264	880-881	,	denotes	,
T12265	882-887	NNS	denotes	lanes
T12266	888-889	CD	denotes	6
T12267	890-891	CD	denotes	8
T12268	891-892	-RRB-	denotes	)
T12269	892-893	.	denotes	.
T12270	2988-2992	JJ	denotes	Next
T12271	2992-2993	,	denotes	,
T12272	2994-2996	PRP	denotes	we
T12273	2997-3006	VBD	denotes	evaluated
T12274	3007-3014	IN	denotes	whether
T12275	3015-3018	NNP	denotes	Tat
T12276	3019-3031	VBD	denotes	counteracted
T12277	3032-3035	DT	denotes	the
T12278	3036-3050	NNP	denotes	IκB-α-mediated
T12279	3051-3061	NN	denotes	inhibition
T12280	3062-3064	IN	denotes	of
T12281	3065-3068	CD	denotes	p65
T12282	3069-3076	JJ	denotes	binding
T12283	3077-3079	TO	denotes	to
T12284	3080-3083	NNP	denotes	DNA
T12285	3084-3086	IN	denotes	by
T12286	3087-3097	VBG	denotes	incubating
T12287	3098-3100	IN	denotes	in
T12288	3101-3106	NN	denotes	vitro
T12289	3107-3117	VBN	denotes	translated
T12290	3118-3121	NNS	denotes	p65
T12291	3122-3125	CC	denotes	and
T12292	3126-3131	JJ	denotes	IκB-α
T12293	3132-3140	NNS	denotes	proteins
T12294	3141-3145	IN	denotes	with
T12295	3146-3149	DT	denotes	the
T12296	3150-3155	NNP	denotes	NF-κB
T12297	3156-3161	NN	denotes	probe
T12298	3162-3164	IN	denotes	in
T12299	3165-3173	NN	denotes	presence
T12300	3174-3176	CC	denotes	or
T12301	3177-3184	NN	denotes	absence
T12302	3185-3187	IN	denotes	of
T12303	3188-3191	NNP	denotes	Tat
T12304	3192-3200	VBD	denotes	followed
T12305	3201-3203	IN	denotes	by
T12306	3204-3208	NNP	denotes	EMSA
T12307	3208-3209	.	denotes	.
T12308	3210-3213	DT	denotes	The
T12309	3214-3217	CD	denotes	p65
T12310	3218-3221	NNP	denotes	DNA
T12311	3222-3229	JJ	denotes	binding
T12312	3230-3238	NN	denotes	activity
T12313	3239-3242	VBD	denotes	was
T12314	3243-3252	VBN	denotes	inhibited
T12315	3253-3255	IN	denotes	by
T12316	3256-3261	NNP	denotes	IκB-α
T12317	3262-3265	CC	denotes	and
T12318	3266-3274	VBN	denotes	restored
T12319	3275-3277	IN	denotes	in
T12320	3278-3279	DT	denotes	a
T12321	3280-3294	JJ	denotes	dose-dependent
T12322	3295-3301	NN	denotes	manner
T12323	3302-3304	IN	denotes	by
T12324	3305-3314	JJ	denotes	wild-type
T12325	3315-3318	NNP	denotes	Tat
T12326	3318-3319	,	denotes	,
T12327	3320-3323	CC	denotes	and
T12328	3324-3327	RB	denotes	not
T12329	3328-3330	IN	denotes	by
T12330	3331-3334	NNP	denotes	Tat
T12331	3335-3336	NNP	denotes	R
T12332	3336-3337	-LRB-	denotes	(
T12333	3337-3339	CD	denotes	49
T12334	3340-3342	CD	denotes	57
T12335	3342-3343	-RRB-	denotes	)
T12336	3344-3345	-LRB-	denotes	(
T12337	3345-3351	NNP	denotes	Figure
T12338	3352-3354	NNP	denotes	3D
T12339	3354-3355	-RRB-	denotes	)
T12340	3355-3356	.	denotes	.
T12341	3357-3364	RB	denotes	Further
T12342	3364-3365	,	denotes	,
T12343	3366-3368	PRP	denotes	we
T12344	3369-3377	VBD	denotes	analysed
T12345	3378-3381	DT	denotes	the
T12346	3382-3385	NNP	denotes	Tat
T12347	3386-3392	NN	denotes	effect
T12348	3393-3395	IN	denotes	on
T12349	3396-3399	DT	denotes	the
T12350	3400-3405	JJ	denotes	IκB-α
T12351	3406-3416	NN	denotes	repression
T12352	3417-3419	IN	denotes	of
T12353	3420-3423	CD	denotes	p65
T12354	3424-3439	JJ	denotes	transcriptional
T12355	3440-3448	NN	denotes	activity
T12356	3449-3451	IN	denotes	by
T12357	3452-3464	VBG	denotes	transfecting
T12358	3465-3468	CD	denotes	p50
T12359	3468-3469	FW	denotes	−
T12360	3469-3470	FW	denotes	/
T12361	3470-3471	FW	denotes	−
T12362	3471-3474	FW	denotes	p65
T12363	3474-3475	FW	denotes	−
T12364	3475-3476	FW	denotes	/
T12365	3476-3477	FW	denotes	−
T12366	3477-3481	FW	denotes	MEFs
T12367	3482-3486	IN	denotes	with
T12368	3487-3490	DT	denotes	the
T12369	3491-3500	JJ	denotes	NF-κB-Luc
T12370	3501-3509	NN	denotes	reporter
T12371	3510-3518	RB	denotes	together
T12372	3519-3523	IN	denotes	with
T12373	3524-3534	NN	denotes	expression
T12374	3535-3542	NNS	denotes	vectors
T12375	3543-3545	IN	denotes	of
T12376	3546-3549	CD	denotes	p65
T12377	3550-3553	CC	denotes	and
T12378	3554-3559	NNP	denotes	IκB-α
T12379	3559-3560	,	denotes	,
T12380	3561-3563	IN	denotes	in
T12381	3564-3572	NN	denotes	presence
T12382	3573-3575	CC	denotes	or
T12383	3576-3583	NN	denotes	absence
T12384	3584-3586	IN	denotes	of
T12385	3587-3590	NNP	denotes	Tat
T12386	3590-3591	.	denotes	.
T12387	3592-3597	JJ	denotes	IκB-α
T12388	3598-3607	VBD	denotes	inhibited
T12389	3608-3611	DT	denotes	the
T12390	3612-3625	JJ	denotes	p65-dependent
T12391	3626-3636	NN	denotes	expression
T12392	3637-3639	IN	denotes	of
T12393	3640-3643	DT	denotes	the
T12394	3644-3654	NN	denotes	luciferase
T12395	3655-3659	NN	denotes	gene
T12396	3659-3660	,	denotes	,
T12397	3661-3666	WDT	denotes	which
T12398	3667-3670	VBD	denotes	was
T12399	3671-3679	VBN	denotes	restored
T12400	3680-3682	IN	denotes	in
T12401	3683-3684	DT	denotes	a
T12402	3685-3699	JJ	denotes	dose-dependent
T12403	3700-3706	NN	denotes	manner
T12404	3707-3709	IN	denotes	by
T12405	3710-3719	JJ	denotes	wild-type
T12406	3720-3723	NNP	denotes	Tat
T12407	3723-3724	,	denotes	,
T12408	3725-3728	CC	denotes	and
T12409	3729-3732	RB	denotes	not
T12410	3733-3735	IN	denotes	by
T12411	3736-3739	NNP	denotes	Tat
T12412	3740-3741	NNP	denotes	R
T12413	3741-3742	-LRB-	denotes	(
T12414	3742-3744	CD	denotes	49
T12415	3745-3747	CD	denotes	57
T12416	3747-3748	-RRB-	denotes	)
T12417	3748-3749	DT	denotes	A
T12418	3750-3751	-LRB-	denotes	(
T12419	3751-3757	NN	denotes	Figure
T12420	3758-3760	CD	denotes	3E
T12421	3760-3761	-RRB-	denotes	)
T12422	3761-3762	.	denotes	.
T12423	3763-3773	RB	denotes	Altogether
T12424	3774-3779	DT	denotes	these
T12425	3780-3787	NNS	denotes	results
T12426	3788-3797	VBD	denotes	indicated
T12427	3798-3802	IN	denotes	that
T12428	3803-3806	NNP	denotes	Tat
T12429	3807-3818	VBZ	denotes	counteracts
T12430	3819-3822	DT	denotes	the
T12431	3823-3828	JJ	denotes	IκB-α
T12432	3829-3839	NN	denotes	repression
T12433	3840-3842	IN	denotes	of
T12434	3843-3846	DT	denotes	the
T12435	3847-3850	CD	denotes	p65
T12436	3851-3862	JJ	denotes	DNA-binding
T12437	3863-3866	CC	denotes	and
T12438	3867-3882	JJ	denotes	transcriptional
T12439	3883-3891	NN	denotes	activity
T12440	3892-3894	IN	denotes	by
T12441	3895-3906	VBG	denotes	associating
T12442	3907-3911	IN	denotes	with
T12443	3912-3917	NNP	denotes	IκB-α
T12444	3918-3921	CC	denotes	and
T12445	3922-3931	VBG	denotes	competing
T12446	3932-3935	DT	denotes	the
T12447	3936-3945	NN	denotes	repressor
T12448	3946-3953	JJ	denotes	binding
T12449	3954-3956	TO	denotes	to
T12450	3957-3960	NNS	denotes	p65
T12451	3960-3961	.	denotes	.
T25677	904-907	NNP	denotes	Tat
T25678	908-916	VBZ	denotes	relieves
T25679	917-920	CD	denotes	p65
T25680	921-925	IN	denotes	from
T25681	926-929	DT	denotes	the
T25682	930-935	JJ	denotes	IκB-α
T25683	936-946	NN	denotes	inhibition
T25684	946-947	.	denotes	.
T25685	948-949	-LRB-	denotes	(
T25686	949-950	NN	denotes	A
T25687	950-951	-RRB-	denotes	)
T25688	952-961	JJ	denotes	Schematic
T25689	962-976	NN	denotes	representation
T25690	977-979	IN	denotes	of
T25691	980-989	JJ	denotes	wild-type
T25692	990-993	CC	denotes	and
T25693	994-1000	JJ	denotes	mutant
T25694	1001-1004	NNP	denotes	Tat
T25695	1005-1013	NNS	denotes	proteins
T25696	1013-1014	.	denotes	.
T25697	1015-1016	-LRB-	denotes	(
T25698	1016-1017	NNP	denotes	B
T25699	1017-1018	-RRB-	denotes	)
T25700	1019-1027	NNP	denotes	HA-IκB-α
T25701	1028-1029	-LRB-	denotes	(
T25702	1029-1030	CD	denotes	5
T25703	1031-1033	NN	denotes	µl
T25704	1033-1034	-RRB-	denotes	)
T25705	1035-1038	VBD	denotes	was
T25706	1039-1048	VBN	denotes	incubated
T25707	1049-1051	IN	denotes	in
T25708	1052-1060	NN	denotes	presence
T25709	1061-1063	CC	denotes	or
T25710	1064-1071	NN	denotes	absence
T25711	1072-1074	IN	denotes	of
T25712	1075-1083	NNP	denotes	FLAG-Tat
T25713	1083-1084	,	denotes	,
T25714	1085-1093	NNP	denotes	FLAG-Tat
T25715	1094-1095	NNP	denotes	T
T25716	1095-1096	,	denotes	,
T25717	1296-1297	:	denotes	;
T25718	1096-1097	NNP	denotes	N
T25719	1097-1098	-LRB-	denotes	(
T25720	1098-1103	CD	denotes	23,24
T25721	1103-1104	-RRB-	denotes	)
T25722	1104-1105	DT	denotes	A
T25723	1105-1106	,	denotes	,
T25724	1107-1115	JJ	denotes	FLAG-Tat
T25725	1116-1117	NNP	denotes	K
T25726	1117-1118	-LRB-	denotes	(
T25727	1118-1123	CD	denotes	50,51
T25728	1123-1124	-RRB-	denotes	)
T25729	1124-1125	DT	denotes	A
T25730	1125-1126	,	denotes	,
T25731	1127-1135	JJ	denotes	FLAG-Tat
T25732	1136-1137	NN	denotes	R
T25733	1137-1138	-LRB-	denotes	(
T25734	1138-1140	CD	denotes	49
T25735	1141-1143	CD	denotes	57
T25736	1143-1144	-RRB-	denotes	)
T25737	1144-1145	DT	denotes	A
T25738	1146-1148	CC	denotes	or
T25739	1149-1157	JJ	denotes	FLAG-Tat
T25740	1158-1159	NNP	denotes	C
T25741	1159-1160	-LRB-	denotes	(
T25742	1160-1168	CD	denotes	22,25,27
T25743	1168-1169	-RRB-	denotes	)
T25744	1169-1170	DT	denotes	A
T25745	1171-1172	-LRB-	denotes	(
T25746	1172-1174	CD	denotes	10
T25747	1175-1177	NN	denotes	µl
T25748	1177-1178	-RRB-	denotes	)
T25749	1179-1184	VBG	denotes	using
T25750	1185-1187	IN	denotes	in
T25751	1188-1193	NN	denotes	vitro
T25752	1194-1204	VBN	denotes	translated
T25753	1205-1213	NNS	denotes	proteins
T25754	1213-1214	.	denotes	.
T25755	1215-1224	JJ	denotes	Wild-type
T25756	1225-1228	CC	denotes	and
T25757	1229-1235	JJ	denotes	mutant
T25758	1236-1239	NNP	denotes	Tat
T25759	1240-1248	NNS	denotes	proteins
T25760	1249-1253	VBD	denotes	were
T25761	1254-1272	VBN	denotes	immunoprecipitated
T25762	1273-1277	IN	denotes	with
T25763	1278-1287	JJ	denotes	anti-FLAG
T25764	1288-1296	NN	denotes	antibody
T25765	1298-1313	NNS	denotes	immunocomplexes
T25766	1314-1318	VBD	denotes	were
T25767	1319-1328	VBN	denotes	separated
T25768	1329-1331	IN	denotes	by
T25769	1332-1334	CD	denotes	12
T25770	1334-1335	NN	denotes	%
T25771	1336-1339	NNP	denotes	SDS
T25772	1340-1344	NNP	denotes	PAGE
T25773	1345-1348	CC	denotes	and
T25774	1349-1357	VBN	denotes	analysed
T25775	1358-1360	IN	denotes	by
T25776	1361-1368	JJ	denotes	western
T25777	1369-1377	VBG	denotes	blotting
T25778	1378-1382	IN	denotes	with
T25779	1383-1390	JJ	denotes	anti-HA
T25780	1391-1394	CC	denotes	and
T25781	1395-1404	JJ	denotes	anti-FLAG
T25782	1405-1415	NNS	denotes	antibodies
T25783	1415-1416	.	denotes	.
T25784	1417-1418	-LRB-	denotes	(
T25785	1418-1419	$	denotes	C
T25786	1419-1420	-RRB-	denotes	)
T25787	1421-1443	JJ	denotes	35S-methionine-labeled
T25788	1444-1447	NNS	denotes	p65
T25789	1448-1449	-LRB-	denotes	(
T25790	1449-1450	CD	denotes	5
T25791	1451-1453	NN	denotes	µl
T25792	1453-1454	-RRB-	denotes	)
T25793	1455-1458	VBD	denotes	was
T25794	1459-1468	VBN	denotes	incubated
T25795	1469-1473	IN	denotes	with
T25796	1474-1476	IN	denotes	in
T25797	1477-1482	NN	denotes	vitro
T25798	1483-1493	VBN	denotes	translated
T25799	1494-1502	NNP	denotes	HA-IκB-α
T25800	1503-1504	-LRB-	denotes	(
T25801	1504-1505	CD	denotes	5
T25802	1506-1508	NN	denotes	µl
T25803	1508-1509	-RRB-	denotes	)
T25804	1510-1512	IN	denotes	in
T25805	1513-1521	NN	denotes	presence
T25806	1522-1524	CC	denotes	or
T25807	1525-1532	NN	denotes	absence
T25808	1533-1535	IN	denotes	of
T25809	1536-1544	NN	denotes	FLAG-Tat
T25810	1545-1547	CC	denotes	or
T25811	1548-1556	NN	denotes	FLAG-Tat
T25812	1557-1558	NN	denotes	R
T25813	1558-1559	-LRB-	denotes	(
T25814	1559-1561	CD	denotes	49
T25815	1562-1564	CD	denotes	57
T25816	1564-1565	-RRB-	denotes	)
T25817	1565-1566	DT	denotes	A
T25818	1567-1568	-LRB-	denotes	(
T25819	1568-1569	CD	denotes	5
T25820	1569-1570	,	denotes	,
T25821	1571-1573	CD	denotes	10
T25822	1574-1576	CC	denotes	or
T25823	1577-1579	CD	denotes	20
T25824	1580-1582	NN	denotes	µl
T25825	1582-1583	-RRB-	denotes	)
T25826	1583-1584	.	denotes	.
T25827	1585-1593	NNP	denotes	HA-IκB-α
T25828	1594-1597	VBD	denotes	was
T25829	1598-1616	VBN	denotes	immunoprecipitated
T25830	1617-1621	IN	denotes	with
T25831	1622-1629	JJ	denotes	anti-HA
T25832	1630-1638	NN	denotes	antibody
T25833	1638-1639	:	denotes	;
T25834	1640-1655	NNS	denotes	immunocomplexes
T25835	1656-1660	VBD	denotes	were
T25836	1661-1670	VBN	denotes	separated
T25837	1671-1673	IN	denotes	by
T25838	1674-1676	CD	denotes	12
T25839	1676-1677	NN	denotes	%
T25840	1678-1681	NNP	denotes	SDS
T25841	1682-1686	NNP	denotes	PAGE
T25842	1687-1690	CC	denotes	and
T25843	1691-1699	VBN	denotes	analysed
T25844	1700-1702	IN	denotes	by
T25845	1703-1710	JJ	denotes	western
T25846	1711-1719	VBG	denotes	blotting
T25847	1720-1724	IN	denotes	with
T25848	1725-1732	JJ	denotes	anti-HA
T25849	1733-1736	CC	denotes	and
T25850	1737-1746	JJ	denotes	anti-FLAG
T25851	1747-1757	NNS	denotes	antibodies
T25852	1757-1758	,	denotes	,
T25853	1759-1761	CC	denotes	or
T25854	1762-1777	NN	denotes	autoradiography
T25855	1778-1779	-LRB-	denotes	(
T25856	1779-1790	NNP	denotes	35S-Met-p65
T25857	1790-1791	-RRB-	denotes	)
T25858	1791-1792	.	denotes	.
T25859	1793-1805	NNP	denotes	Densitometry
T25860	1806-1812	NNS	denotes	values
T25861	1813-1814	-LRB-	denotes	(
T25862	1814-1815	NNP	denotes	D
T25863	1815-1816	-RRB-	denotes	)
T25864	1817-1819	IN	denotes	of
T25865	1820-1823	DT	denotes	the
T25866	1824-1829	NNS	denotes	bands
T25867	1830-1834	VBD	denotes	were
T25868	1835-1844	VBN	denotes	expressed
T25869	1845-1847	IN	denotes	as
T25870	1848-1852	VBP	denotes	fold
T25871	1853-1861	NN	denotes	increase
T25872	1862-1867	IN	denotes	above
T25873	1868-1871	DT	denotes	the
T25874	1872-1879	NN	denotes	control
T25875	1880-1881	-LRB-	denotes	(
T25876	1881-1885	NN	denotes	lane
T25877	1886-1887	CD	denotes	1
T25878	1887-1888	-RRB-	denotes	)
T25879	1888-1889	.	denotes	.
T25880	1890-1891	-LRB-	denotes	(
T25881	1891-1892	NNP	denotes	D
T25882	1892-1893	-RRB-	denotes	)
T25883	1894-1897	DT	denotes	The
T25884	1898-1901	CD	denotes	p65
T25885	1902-1905	NNP	denotes	DNA
T25886	1906-1913	JJ	denotes	binding
T25887	1914-1922	NN	denotes	activity
T25888	1923-1926	VBD	denotes	was
T25889	1927-1935	VBN	denotes	analysed
T25890	1936-1938	IN	denotes	by
T25891	1939-1943	NNP	denotes	EMSA
T25892	1944-1949	VBG	denotes	using
T25893	1950-1952	IN	denotes	in
T25894	1953-1958	NN	denotes	vitro
T25895	1959-1969	VBN	denotes	translated
T25896	1970-1978	NNS	denotes	proteins
T25897	1978-1979	.	denotes	.
T25898	1980-1997	JJ	denotes	32P-labeled-NF-κB
T25899	1998-2013	JJ	denotes	double-stranded
T25900	2014-2029	NN	denotes	oligonucleotide
T25901	2030-2033	VBD	denotes	was
T25902	2034-2043	VBN	denotes	incubated
T25903	2044-2048	IN	denotes	with
T25904	2049-2052	NNS	denotes	p65
T25905	2053-2054	-LRB-	denotes	(
T25906	2054-2057	CD	denotes	0.5
T25907	2058-2060	NN	denotes	µl
T25908	2060-2061	-RRB-	denotes	)
T25909	2062-2064	IN	denotes	in
T25910	2065-2073	NN	denotes	presence
T25911	2074-2076	CC	denotes	or
T25912	2077-2084	NN	denotes	absence
T25913	2085-2087	IN	denotes	of
T25914	2088-2096	NNP	denotes	HA-IκB-α
T25915	2097-2098	-LRB-	denotes	(
T25916	2098-2099	CD	denotes	1
T25917	2100-2102	NN	denotes	µl
T25918	2102-2103	-RRB-	denotes	)
T25919	2103-2104	,	denotes	,
T25920	2105-2113	JJ	denotes	FLAG-Tat
T25921	2114-2116	CC	denotes	or
T25922	2117-2125	JJ	denotes	FLAG-Tat
T25923	2126-2127	NN	denotes	R
T25924	2127-2128	-LRB-	denotes	(
T25925	2128-2130	CD	denotes	49
T25926	2131-2133	CD	denotes	57
T25927	2133-2134	-RRB-	denotes	)
T25928	2134-2135	DT	denotes	A
T25929	2136-2137	-LRB-	denotes	(
T25930	2137-2138	CD	denotes	5
T25931	2139-2142	CC	denotes	and
T25932	2143-2145	CD	denotes	10
T25933	2146-2148	NN	denotes	µl
T25934	2148-2149	-RRB-	denotes	)
T25935	2149-2150	:	denotes	;
T25936	2151-2162	NN	denotes	competition
T25937	2163-2165	IN	denotes	of
T25938	2166-2169	NNP	denotes	DNA
T25939	2170-2177	JJ	denotes	binding
T25940	2178-2181	VBD	denotes	was
T25941	2182-2191	VBN	denotes	performed
T25942	2192-2196	IN	denotes	with
T25943	2197-2199	CD	denotes	10
T25944	2200-2201	:	denotes	-
T25945	2202-2204	RP	denotes	up
T25946	2205-2207	TO	denotes	to
T25947	2208-2216	JJ	denotes	100-fold
T25948	2217-2222	NN	denotes	molar
T25949	2223-2229	NN	denotes	excess
T25950	2230-2232	IN	denotes	of
T25951	2233-2242	JJ	denotes	unlabeled
T25952	2243-2248	JJ	denotes	NF-κB
T25953	2249-2264	JJ	denotes	double-stranded
T25954	2265-2280	NN	denotes	oligonucleotide
T25955	2280-2281	.	denotes	.
T25956	2282-2293	NNP	denotes	DNA/protein
T25957	2294-2303	NNS	denotes	complexes
T25958	2304-2308	VBD	denotes	were
T25959	2309-2312	VBN	denotes	run
T25960	2313-2315	IN	denotes	on
T25961	2316-2317	CD	denotes	6
T25962	2317-2318	NN	denotes	%
T25963	2319-2323	NN	denotes	PAGE
T25964	2324-2327	NNP	denotes	TBE
T25965	2328-2331	CC	denotes	and
T25966	2332-2340	VBN	denotes	analysed
T25967	2341-2343	IN	denotes	by
T25968	2344-2359	NN	denotes	autoradiography
T25969	2359-2360	.	denotes	.
T25970	2361-2362	-LRB-	denotes	(
T25971	2362-2363	NNP	denotes	E
T25972	2363-2364	-RRB-	denotes	)
T25973	2365-2368	FW	denotes	p50
T25974	2368-2369	FW	denotes	−
T25975	2369-2370	FW	denotes	/
T25976	2370-2371	FW	denotes	−
T25977	2371-2374	FW	denotes	p65
T25978	2374-2375	FW	denotes	−
T25979	2375-2376	FW	denotes	/
T25980	2376-2377	FW	denotes	−
T25981	2377-2381	NNS	denotes	MEFs
T25982	2382-2383	-LRB-	denotes	(
T25983	2383-2384	CD	denotes	3
T25984	2385-2386	CD	denotes	×
T25985	2387-2390	CD	denotes	105
T25986	2391-2396	NNS	denotes	cells
T25987	2396-2397	-RRB-	denotes	)
T25988	2398-2402	VBD	denotes	were
T25989	2403-2414	VBN	denotes	transfected
T25990	2415-2419	IN	denotes	with
T25991	2420-2426	NNP	denotes	pκBLuc
T25992	2427-2428	-LRB-	denotes	(
T25993	2428-2431	CD	denotes	0.5
T25994	2432-2434	NN	denotes	µg
T25995	2434-2435	-RRB-	denotes	)
T25996	2436-2439	CC	denotes	and
T25997	2440-2449	JJ	denotes	pSV-β-Gal
T25998	2450-2451	-LRB-	denotes	(
T25999	2451-2454	CD	denotes	0.1
T26000	2455-2457	NN	denotes	µg
T26001	2457-2458	-RRB-	denotes	)
T26002	2459-2463	IN	denotes	with
T26003	2464-2466	CC	denotes	or
T26004	2467-2474	IN	denotes	without
T26005	2475-2484	NNP	denotes	pRc/CMV-p
T26006	2484-2486	CD	denotes	65
T26007	2487-2488	-LRB-	denotes	(
T26008	2488-2491	CD	denotes	0.5
T26009	2492-2494	NN	denotes	µg
T26010	2494-2495	-RRB-	denotes	)
T26011	2495-2496	,	denotes	,
T26012	2497-2511	NN	denotes	pCMV4-HA-IκB-α
T26013	2512-2513	-LRB-	denotes	(
T26014	2513-2516	CD	denotes	0.5
T26015	2517-2519	NN	denotes	µg
T26016	2519-2520	-RRB-	denotes	)
T26017	2520-2521	,	denotes	,
T26018	2522-2533	NN	denotes	p3xFLAG-Tat
T26019	2534-2536	CC	denotes	or
T26020	2537-2548	NN	denotes	p3xFLAG-Tat
T26021	2549-2550	NN	denotes	R
T26022	2550-2551	-LRB-	denotes	(
T26023	2551-2553	CD	denotes	49
T26024	2554-2556	CD	denotes	57
T26025	2556-2557	-RRB-	denotes	)
T26026	2557-2558	DT	denotes	A
T26027	2559-2560	-LRB-	denotes	(
T26028	2560-2563	CD	denotes	0.5
T26029	2563-2564	,	denotes	,
T26030	2565-2566	CD	denotes	1
T26031	2567-2570	CC	denotes	and
T26032	2571-2572	CD	denotes	2
T26033	2573-2575	NN	denotes	µg
T26034	2575-2576	-RRB-	denotes	)
T26035	2576-2577	.	denotes	.
T26036	2578-2581	DT	denotes	The
T26037	2582-2592	NN	denotes	luciferase
T26038	2593-2601	NN	denotes	activity
T26039	2602-2605	VBD	denotes	was
T26040	2606-2614	VBN	denotes	measured
T26041	2615-2617	IN	denotes	in
T26042	2618-2622	NN	denotes	cell
T26043	2623-2631	NNS	denotes	extracts
T26044	2632-2636	JJ	denotes	48-h
T26045	2637-2654	NN	denotes	post-transfection
T26046	2655-2658	CC	denotes	and
T26047	2659-2669	VBN	denotes	normalized
T26048	2670-2672	TO	denotes	to
T26049	2673-2688	JJ	denotes	β-galactosidase
T26050	2689-2697	NN	denotes	activity
T26051	2697-2698	.	denotes	.
T26052	2699-2703	VB	denotes	Fold
T26053	2704-2714	NN	denotes	activation
T26054	2715-2718	VBD	denotes	was
T26055	2719-2729	VBN	denotes	calculated
T26056	2730-2738	JJ	denotes	relative
T26057	2739-2741	TO	denotes	to
T26058	2742-2754	NN	denotes	transfection
T26059	2755-2757	IN	denotes	of
T26060	2758-2761	DT	denotes	the
T26061	2762-2768	NNP	denotes	pκBLuc
T26062	2769-2776	NN	denotes	plasmid
T26063	2777-2782	RB	denotes	alone
T26064	2782-2783	.	denotes	.
T26065	2784-2790	NNS	denotes	Values
T26066	2791-2792	-LRB-	denotes	(
T26067	2792-2796	JJ	denotes	mean
T26068	2797-2798	NN	denotes	±
T26069	2799-2801	NNP	denotes	SE
T26070	2801-2802	,	denotes	,
T26071	2803-2804	JJ	denotes	n
T26072	2805-2806	SYM	denotes	=
T26073	2807-2808	LS	denotes	3
T26074	2808-2809	-RRB-	denotes	)
T26075	2810-2813	VBP	denotes	are
T26076	2814-2819	VBN	denotes	shown
T26077	2819-2820	.	denotes	.
T26078	2821-2823	IN	denotes	As
T26079	2824-2831	NN	denotes	control
T26080	2832-2834	IN	denotes	of
T26081	2835-2842	NN	denotes	protein
T26082	2843-2853	NN	denotes	expression
T26083	2853-2854	,	denotes	,
T26084	2855-2863	NNS	denotes	aliquots
T26085	2864-2866	IN	denotes	of
T26086	2867-2871	NN	denotes	cell
T26087	2872-2880	NNS	denotes	extracts
T26088	2881-2882	-LRB-	denotes	(
T26089	2882-2884	CD	denotes	20
T26090	2885-2887	NN	denotes	µg
T26091	2887-2888	-RRB-	denotes	)
T26092	2889-2893	VBD	denotes	were
T26093	2894-2902	VBN	denotes	analysed
T26094	2903-2905	IN	denotes	by
T26095	2906-2913	JJ	denotes	western
T26096	2914-2922	VBG	denotes	blotting
T26097	2923-2927	IN	denotes	with
T26098	2928-2936	JJ	denotes	anti-p65
T26099	2936-2937	,	denotes	,
T26100	2938-2945	JJ	denotes	anti-HA
T26101	2945-2946	,	denotes	,
T26102	2947-2956	JJ	denotes	anti-FLAG
T26103	2957-2960	CC	denotes	and
T26104	2961-2975	JJ	denotes	anti-γ-tubulin
T26105	2976-2986	NNS	denotes	antibodies
T26106	2986-2987	.	denotes	.
R9817	T12077	T12078	nsubj	Tat,counteracts
R9818	T12078	T12078	ROOT	counteracts,counteracts
R9819	T12079	T12081	det	the,inhibition
R9820	T12080	T12081	amod	IκB-α,inhibition
R9821	T12081	T12078	dobj	inhibition,counteracts
R9822	T12082	T12081	prep	of,inhibition
R9823	T12083	T12082	pobj	p65,of
R9824	T12084	T12078	prep	by,counteracts
R9825	T12085	T12084	pcomp	competing,by
R9826	T12086	T12087	det	the,repressor
R9827	T12087	T12088	compound	repressor,binding
R9828	T12088	T12091	advmod	binding,investigated
R9829	T12089	T12091	nsubj	We,investigated
R9830	T12090	T12091	advmod	further,investigated
R9831	T12091	T12078	conj	investigated,counteracts
R9832	T12092	T12094	det	the,interaction
R9833	T12093	T12094	amod	physical,interaction
R9834	T12094	T12091	dobj	interaction,investigated
R9835	T12095	T12094	prep	of,interaction
R9836	T12096	T12095	pobj	Tat,of
R9837	T12097	T12094	prep	with,interaction
R9838	T12098	T12097	pobj	IκB-α,with
R9839	T12099	T12097	pcomp	using,with
R9840	T12100	T12099	prep	in,using
R9841	T12101	T12103	amod	vitro,proteins
R9842	T12102	T12103	amod	translated,proteins
R9843	T12103	T12100	pobj	proteins,in
R9844	T12104	T12099	prep	in,using
R9845	T12105	T12106	compound	coimmunoprecipitation,assays
R9846	T12106	T12104	pobj	assays,in
R9847	T12107	T12078	punct	.,counteracts
R9848	T12108	T12115	prep	For,used
R9849	T12109	T12108	pcomp	mapping,For
R9850	T12110	T12112	det	the,domains
R9851	T12111	T12112	compound	interaction,domains
R9852	T12112	T12109	dobj	domains,mapping
R9853	T12113	T12115	punct	",",used
R9854	T12114	T12115	nsubj	we,used
R9855	T12115	T12115	ROOT	used,used
R9856	T12116	T12119	det	the,mutants
R9857	T12117	T12119	amod	following,mutants
R9858	T12118	T12119	compound	Tat,mutants
R9859	T12119	T12115	dobj	mutants,used
R9860	T12120	T12119	punct	:,mutants
R9861	T12121	T12122	compound	Tat,T
R9862	T12122	T12119	appos	T,mutants
R9863	T12123	T12122	punct	",",T
R9864	T12124	T12122	conj	N,T
R9865	T12125	T12124	punct	(,N
R9866	T12126	T12124	appos	"23,24",N
R9867	T12127	T12124	punct	),N
R9868	T12128	T12131	det	A,C
R9869	T12129	T12131	punct	",",C
R9870	T12130	T12131	compound	Tat,C
R9871	T12131	T12131	ROOT	C,C
R9872	T12132	T12131	punct	(,C
R9873	T12133	T12131	appos	"22,25,27",C
R9874	T12134	T12131	punct	),C
R9875	T12135	T12142	det	A,A
R9876	T12136	T12142	punct	",",A
R9877	T12137	T12138	compound	Tat,K
R9878	T12138	T12142	nmod	K,A
R9879	T12139	T12138	punct	(,K
R9880	T12140	T12138	appos	"50,51",K
R9881	T12141	T12142	punct	),A
R9882	T12142	T12142	ROOT	A,A
R9883	T12143	T12142	cc	and,A
R9884	T12144	T12145	compound	Tat,R
R9885	T12145	T12142	conj	R,A
R9886	T12146	T12145	punct	(,R
R9887	T12147	T12145	appos	49,R
R9888	T12148	T12145	appos	57,R
R9889	T12149	T12145	punct	),R
R9890	T12150	T12153	det	A,3A
R9891	T12151	T12153	punct	(,3A
R9892	T12152	T12153	compound	Figure,3A
R9893	T12153	T12153	ROOT	3A,3A
R9894	T12154	T12153	punct	),3A
R9895	T12155	T12153	punct	.,3A
R9896	T12156	T12157	amod	IκB-α,coimmunoprecipitated
R9897	T12157	T12157	ROOT	coimmunoprecipitated,coimmunoprecipitated
R9898	T12158	T12157	prep	with,coimmunoprecipitated
R9899	T12159	T12161	det	all,proteins
R9900	T12160	T12161	compound	Tat,proteins
R9901	T12161	T12158	pobj	proteins,with
R9902	T12162	T12157	punct	",",coimmunoprecipitated
R9903	T12163	T12157	prep	except,coimmunoprecipitated
R9904	T12164	T12165	compound	Tat,R
R9905	T12165	T12163	pobj	R,except
R9906	T12166	T12165	punct	(,R
R9907	T12167	T12165	appos	49,R
R9908	T12168	T12165	appos	57,R
R9909	T12169	T12165	punct	),R
R9910	T12170	T12173	det	A,3B
R9911	T12171	T12173	punct	(,3B
R9912	T12172	T12173	compound	Figure,3B
R9913	T12173	T12173	ROOT	3B,3B
R9914	T12174	T12173	punct	),3B
R9915	T12175	T12173	punct	.,3B
R9916	T12176	T12177	det	These,results
R9917	T12177	T12178	nsubj	results,indicated
R9918	T12178	T12178	ROOT	indicated,indicated
R9919	T12179	T12186	mark	that,involved
R9920	T12180	T12182	det	the,domain
R9921	T12181	T12182	amod	arginine-rich,domain
R9922	T12182	T12186	nsubjpass	domain,involved
R9923	T12183	T12182	prep	of,domain
R9924	T12184	T12183	pobj	Tat,of
R9925	T12185	T12186	auxpass	was,involved
R9926	T12186	T12178	ccomp	involved,indicated
R9927	T12187	T12186	prep	in,involved
R9928	T12188	T12189	det	the,binding
R9929	T12189	T12187	pobj	binding,in
R9930	T12190	T12189	prep	to,binding
R9931	T12191	T12190	pobj	IκB-α,to
R9932	T12192	T12191	punct	",",IκB-α
R9933	T12193	T12194	nsubj	which,was
R9934	T12194	T12191	relcl	was,IκB-α
R9935	T12195	T12194	acomp	consistent,was
R9936	T12196	T12195	prep	with,consistent
R9937	T12197	T12198	amod	previous,observations
R9938	T12198	T12196	pobj	observations,with
R9939	T12199	T12198	punct	(,observations
R9940	T12200	T12198	appos	"50,51",observations
R9941	T12201	T12198	punct	),observations
R9942	T12202	T12178	punct	.,indicated
R9943	T12203	T12209	advmod	Moreover,competed
R9944	T12204	T12209	punct	",",competed
R9945	T12205	T12206	nsubj	Tat,associating
R9946	T12206	T12209	advcl	associating,competed
R9947	T12207	T12206	prep	with,associating
R9948	T12208	T12209	nsubj	IκB-α,competed
R9949	T12209	T12209	ROOT	competed,competed
R9950	T12210	T12211	det	the,binding
R9951	T12211	T12209	dobj	binding,competed
R9952	T12212	T12211	prep	of,binding
R9953	T12213	T12212	pobj	IκB-α,of
R9954	T12214	T12215	aux	to,p65
R9955	T12215	T12211	advcl	p65,binding
R9956	T12216	T12215	prep	in,p65
R9957	T12217	T12219	det	a,manner
R9958	T12218	T12219	amod	dose-dependent,manner
R9959	T12219	T12216	pobj	manner,in
R9960	T12220	T12222	punct	(,3C
R9961	T12221	T12222	compound	Figure,3C
R9962	T12222	T12215	dep	3C,p65
R9963	T12223	T12222	punct	",",3C
R9964	T12224	T12215	dobj	lanes,p65
R9965	T12225	T12224	nummod	3,lanes
R9966	T12226	T12224	appos	5,lanes
R9967	T12227	T12209	punct	),competed
R9968	T12228	T12209	punct	",",competed
R9969	T12229	T12231	mark	while,R
R9970	T12230	T12231	compound	Tat,R
R9971	T12231	T12209	advcl	R,competed
R9972	T12232	T12231	punct	(,R
R9973	T12233	T12231	nummod	49,R
R9974	T12234	T12233	prep	57,49
R9975	T12235	T12233	punct	),49
R9976	T12236	T12236	ROOT	A,A
R9977	T12237	T12236	punct	",",A
R9978	T12238	T12239	nsubj	which,lacked
R9979	T12239	T12236	relcl	lacked,A
R9980	T12240	T12242	det	the,site
R9981	T12241	T12242	amod	binding,site
R9982	T12242	T12239	dobj	site,lacked
R9983	T12243	T12242	prep	for,site
R9984	T12244	T12243	pobj	IκB-α,for
R9985	T12245	T12248	punct	",",associate
R9986	T12246	T12248	aux	did,associate
R9987	T12247	T12248	neg	not,associate
R9988	T12248	T12236	appos	associate,A
R9989	T12249	T12248	prep	with,associate
R9990	T12250	T12249	pobj	IκB-α,with
R9991	T12251	T12248	cc	and,associate
R9992	T12252	T12254	aux	did,compete
R9993	T12253	T12254	neg	not,compete
R9994	T12254	T12248	conj	compete,associate
R9995	T12255	T12256	det	the,binding
R9996	T12256	T12254	dobj	binding,compete
R9997	T12257	T12256	prep	of,binding
R9998	T12258	T12257	pobj	IκB-α,of
R9999	T12259	T12260	aux	to,p65
R10000	T12260	T12256	relcl	p65,binding
R10001	T12261	T12263	punct	(,3C
R10002	T12262	T12263	compound	Figure,3C
R10003	T12263	T12260	appos	3C,p65
R10004	T12264	T12263	punct	",",3C
R10005	T12265	T12263	appos	lanes,3C
R10006	T12266	T12265	appos	6,lanes
R10007	T12267	T12265	appos	8,lanes
R10008	T12268	T12265	punct	),lanes
R10009	T12269	T12248	punct	.,associate
R10010	T12270	T12273	advmod	Next,evaluated
R10011	T12271	T12273	punct	",",evaluated
R10012	T12272	T12273	nsubj	we,evaluated
R10013	T12273	T12273	ROOT	evaluated,evaluated
R10014	T12274	T12276	mark	whether,counteracted
R10015	T12275	T12276	nsubj	Tat,counteracted
R10016	T12276	T12273	ccomp	counteracted,evaluated
R10017	T12277	T12279	det	the,inhibition
R10018	T12278	T12279	compound	IκB-α-mediated,inhibition
R10019	T12279	T12276	dobj	inhibition,counteracted
R10020	T12280	T12279	prep	of,inhibition
R10021	T12281	T12280	pobj	p65,of
R10022	T12282	T12276	oprd	binding,counteracted
R10023	T12283	T12282	prep	to,binding
R10024	T12284	T12283	pobj	DNA,to
R10025	T12285	T12276	prep	by,counteracted
R10026	T12286	T12285	pcomp	incubating,by
R10027	T12287	T12286	prep	in,incubating
R10028	T12288	T12290	amod	vitro,p65
R10029	T12289	T12290	amod	translated,p65
R10030	T12290	T12287	pobj	p65,in
R10031	T12291	T12290	cc	and,p65
R10032	T12292	T12293	amod	IκB-α,proteins
R10033	T12293	T12290	conj	proteins,p65
R10034	T12294	T12290	prep	with,p65
R10035	T12295	T12297	det	the,probe
R10036	T12296	T12297	compound	NF-κB,probe
R10037	T12297	T12294	pobj	probe,with
R10038	T12298	T12297	prep	in,probe
R10039	T12299	T12298	pobj	presence,in
R10040	T12300	T12299	cc	or,presence
R10041	T12301	T12299	conj	absence,presence
R10042	T12302	T12301	prep	of,absence
R10043	T12303	T12302	pobj	Tat,of
R10044	T12304	T12285	acl	followed,by
R10045	T12305	T12304	agent	by,followed
R10046	T12306	T12305	pobj	EMSA,by
R10047	T12307	T12273	punct	.,evaluated
R10048	T12308	T12312	det	The,activity
R10049	T12309	T12310	nummod	p65,DNA
R10050	T12310	T12312	nmod	DNA,activity
R10051	T12311	T12312	amod	binding,activity
R10052	T12312	T12314	nsubjpass	activity,inhibited
R10053	T12313	T12314	auxpass	was,inhibited
R10054	T12314	T12314	ROOT	inhibited,inhibited
R10055	T12315	T12314	agent	by,inhibited
R10056	T12316	T12315	pobj	IκB-α,by
R10057	T12317	T12314	cc	and,inhibited
R10058	T12318	T12314	conj	restored,inhibited
R10059	T12319	T12318	prep	in,restored
R10060	T12320	T12322	det	a,manner
R10061	T12321	T12322	amod	dose-dependent,manner
R10062	T12322	T12319	pobj	manner,in
R10063	T12323	T12318	agent	by,restored
R10064	T12324	T12325	amod	wild-type,Tat
R10065	T12325	T12323	pobj	Tat,by
R10066	T12326	T12318	punct	",",restored
R10067	T12327	T12314	cc	and,inhibited
R10068	T12328	T12329	neg	not,by
R10069	T12329	T12314	conj	by,inhibited
R10070	T12330	T12331	compound	Tat,R
R10071	T12331	T12329	pobj	R,by
R10072	T12332	T12331	punct	(,R
R10073	T12333	T12331	appos	49,R
R10074	T12334	T12331	appos	57,R
R10075	T12335	T12331	punct	),R
R10076	T12336	T12338	punct	(,3D
R10077	T12337	T12338	compound	Figure,3D
R10078	T12338	T12331	appos	3D,R
R10079	T12339	T12329	punct	),by
R10080	T12340	T12314	punct	.,inhibited
R10081	T12341	T12344	advmod	Further,analysed
R10082	T12342	T12344	punct	",",analysed
R10083	T12343	T12344	nsubj	we,analysed
R10084	T12344	T12344	ROOT	analysed,analysed
R10085	T12345	T12347	det	the,effect
R10086	T12346	T12347	compound	Tat,effect
R10087	T12347	T12344	dobj	effect,analysed
R10088	T12348	T12347	prep	on,effect
R10089	T12349	T12351	det	the,repression
R10090	T12350	T12351	amod	IκB-α,repression
R10091	T12351	T12348	pobj	repression,on
R10092	T12352	T12351	prep	of,repression
R10093	T12353	T12355	nummod	p65,activity
R10094	T12354	T12355	amod	transcriptional,activity
R10095	T12355	T12352	pobj	activity,of
R10096	T12356	T12344	prep	by,analysed
R10097	T12357	T12356	pcomp	transfecting,by
R10098	T12358	T12359	nummod	p50,−
R10099	T12359	T12366	nmod	−,MEFs
R10100	T12360	T12366	nmod	/,MEFs
R10101	T12361	T12366	nmod	−,MEFs
R10102	T12362	T12366	nmod	p65,MEFs
R10103	T12363	T12366	nmod	−,MEFs
R10104	T12364	T12366	nmod	/,MEFs
R10105	T12365	T12366	nmod	−,MEFs
R10106	T12366	T12357	dobj	MEFs,transfecting
R10107	T12367	T12357	prep	with,transfecting
R10108	T12368	T12370	det	the,reporter
R10109	T12369	T12370	amod	NF-κB-Luc,reporter
R10110	T12370	T12367	pobj	reporter,with
R10111	T12371	T12357	advmod	together,transfecting
R10112	T12372	T12357	prep	with,transfecting
R10113	T12373	T12374	compound	expression,vectors
R10114	T12374	T12372	pobj	vectors,with
R10115	T12375	T12374	prep	of,vectors
R10116	T12376	T12375	pobj	p65,of
R10117	T12377	T12376	cc	and,p65
R10118	T12378	T12376	conj	IκB-α,p65
R10119	T12379	T12344	punct	",",analysed
R10120	T12380	T12344	prep	in,analysed
R10121	T12381	T12380	pobj	presence,in
R10122	T12382	T12381	cc	or,presence
R10123	T12383	T12381	conj	absence,presence
R10124	T12384	T12383	prep	of,absence
R10125	T12385	T12384	pobj	Tat,of
R10126	T12386	T12344	punct	.,analysed
R10127	T12387	T12388	nsubj	IκB-α,inhibited
R10128	T12388	T12388	ROOT	inhibited,inhibited
R10129	T12389	T12391	det	the,expression
R10130	T12390	T12391	amod	p65-dependent,expression
R10131	T12391	T12388	dobj	expression,inhibited
R10132	T12392	T12391	prep	of,expression
R10133	T12393	T12395	det	the,gene
R10134	T12394	T12395	compound	luciferase,gene
R10135	T12395	T12392	pobj	gene,of
R10136	T12396	T12395	punct	",",gene
R10137	T12397	T12399	nsubjpass	which,restored
R10138	T12398	T12399	auxpass	was,restored
R10139	T12399	T12395	relcl	restored,gene
R10140	T12400	T12399	prep	in,restored
R10141	T12401	T12403	det	a,manner
R10142	T12402	T12403	amod	dose-dependent,manner
R10143	T12403	T12400	pobj	manner,in
R10144	T12404	T12399	agent	by,restored
R10145	T12405	T12406	amod	wild-type,Tat
R10146	T12406	T12404	pobj	Tat,by
R10147	T12407	T12399	punct	",",restored
R10148	T12408	T12399	cc	and,restored
R10149	T12409	T12410	neg	not,by
R10150	T12410	T12399	conj	by,restored
R10151	T12411	T12412	compound	Tat,R
R10152	T12412	T12410	pobj	R,by
R10153	T12413	T12412	punct	(,R
R10154	T12414	T12412	appos	49,R
R10155	T12415	T12412	appos	57,R
R10156	T12416	T12412	punct	),R
R10157	T12417	T12419	det	A,Figure
R10158	T12418	T12419	punct	(,Figure
R10159	T12419	T12419	ROOT	Figure,Figure
R10160	T12420	T12419	nummod	3E,Figure
R10161	T12421	T12419	punct	),Figure
R10162	T12422	T12419	punct	.,Figure
R10163	T12423	T12426	advmod	Altogether,indicated
R10164	T12424	T12425	det	these,results
R10165	T12425	T12426	nsubj	results,indicated
R10166	T12426	T12426	ROOT	indicated,indicated
R10167	T12427	T12429	mark	that,counteracts
R10168	T12428	T12429	nsubj	Tat,counteracts
R10169	T12429	T12426	ccomp	counteracts,indicated
R10170	T12430	T12432	det	the,repression
R10171	T12431	T12432	amod	IκB-α,repression
R10172	T12432	T12429	dobj	repression,counteracts
R10173	T12433	T12432	prep	of,repression
R10174	T12434	T12436	det	the,DNA-binding
R10175	T12435	T12436	nummod	p65,DNA-binding
R10176	T12436	T12433	pobj	DNA-binding,of
R10177	T12437	T12436	cc	and,DNA-binding
R10178	T12438	T12436	conj	transcriptional,DNA-binding
R10179	T12439	T12436	conj	activity,DNA-binding
R10180	T12440	T12429	prep	by,counteracts
R10181	T12441	T12440	pcomp	associating,by
R10182	T12442	T12441	prep	with,associating
R10183	T12443	T12442	pobj	IκB-α,with
R10184	T12444	T12441	cc	and,associating
R10185	T12445	T12441	conj	competing,associating
R10186	T12446	T12447	det	the,repressor
R10187	T12447	T12448	nsubj	repressor,binding
R10188	T12448	T12445	ccomp	binding,competing
R10189	T12449	T12448	prep	to,binding
R10190	T12450	T12449	pobj	p65,to
R10191	T12451	T12426	punct	.,indicated
R20432	T25677	T25678	nsubj	Tat,relieves
R20433	T25678	T25678	ROOT	relieves,relieves
R20434	T25679	T25678	dobj	p65,relieves
R20435	T25680	T25678	prep	from,relieves
R20436	T25681	T25683	det	the,inhibition
R20437	T25682	T25683	amod	IκB-α,inhibition
R20438	T25683	T25680	pobj	inhibition,from
R20439	T25684	T25678	punct	.,relieves
R20440	T25685	T25686	punct	(,A
R20441	T25686	T25689	nmod	A,representation
R20442	T25687	T25686	punct	),A
R20443	T25688	T25689	amod	Schematic,representation
R20444	T25689	T25689	ROOT	representation,representation
R20445	T25690	T25689	prep	of,representation
R20446	T25691	T25695	amod	wild-type,proteins
R20447	T25692	T25691	cc	and,wild-type
R20448	T25693	T25691	conj	mutant,wild-type
R20449	T25694	T25695	compound	Tat,proteins
R20450	T25695	T25690	pobj	proteins,of
R20451	T25696	T25689	punct	.,representation
R20452	T25697	T25700	punct	(,HA-IκB-α
R20453	T25698	T25700	nmod	B,HA-IκB-α
R20454	T25699	T25700	punct	),HA-IκB-α
R20455	T25700	T25706	nsubjpass	HA-IκB-α,incubated
R20456	T25701	T25703	punct	(,µl
R20457	T25702	T25703	nummod	5,µl
R20458	T25703	T25700	appos	µl,HA-IκB-α
R20459	T25704	T25703	punct	),µl
R20460	T25705	T25706	auxpass	was,incubated
R20461	T25706	T25706	ROOT	incubated,incubated
R20462	T25707	T25706	prep	in,incubated
R20463	T25708	T25707	pobj	presence,in
R20464	T25709	T25708	cc	or,presence
R20465	T25710	T25708	conj	absence,presence
R20466	T25711	T25710	prep	of,absence
R20467	T25712	T25711	pobj	FLAG-Tat,of
R20468	T25713	T25712	punct	",",FLAG-Tat
R20469	T25714	T25715	compound	FLAG-Tat,T
R20470	T25715	T25712	conj	T,FLAG-Tat
R20471	T25716	T25715	punct	",",T
R20472	T25717	T25767	punct	;,separated
R20473	T25718	T25715	conj	N,T
R20474	T25719	T25720	punct	(,"23,24"
R20475	T25720	T25718	parataxis	"23,24",N
R20476	T25721	T25720	punct	),"23,24"
R20477	T25722	T25725	det	A,K
R20478	T25723	T25725	punct	",",K
R20479	T25724	T25725	compound	FLAG-Tat,K
R20480	T25725	T25725	ROOT	K,K
R20481	T25726	T25725	punct	(,K
R20482	T25727	T25725	appos	"50,51",K
R20483	T25728	T25725	punct	),K
R20484	T25729	T25732	det	A,R
R20485	T25730	T25732	punct	",",R
R20486	T25731	T25732	amod	FLAG-Tat,R
R20487	T25732	T25732	ROOT	R,R
R20488	T25733	T25732	punct	(,R
R20489	T25734	T25732	appos	49,R
R20490	T25735	T25732	appos	57,R
R20491	T25736	T25732	punct	),R
R20492	T25737	T25740	det	A,C
R20493	T25738	T25739	cc	or,FLAG-Tat
R20494	T25739	T25740	compound	FLAG-Tat,C
R20495	T25740	T25740	ROOT	C,C
R20496	T25741	T25740	punct	(,C
R20497	T25742	T25740	appos	"22,25,27",C
R20498	T25743	T25740	punct	),C
R20499	T25744	T25747	det	A,µl
R20500	T25745	T25747	punct	(,µl
R20501	T25746	T25747	nummod	10,µl
R20502	T25747	T25747	ROOT	µl,µl
R20503	T25748	T25747	punct	),µl
R20504	T25749	T25747	acl	using,µl
R20505	T25750	T25749	prep	in,using
R20506	T25751	T25753	amod	vitro,proteins
R20507	T25752	T25753	amod	translated,proteins
R20508	T25753	T25750	pobj	proteins,in
R20509	T25754	T25747	punct	.,µl
R20510	T25755	T25759	amod	Wild-type,proteins
R20511	T25756	T25755	cc	and,Wild-type
R20512	T25757	T25755	conj	mutant,Wild-type
R20513	T25758	T25759	compound	Tat,proteins
R20514	T25759	T25761	nsubjpass	proteins,immunoprecipitated
R20515	T25760	T25761	auxpass	were,immunoprecipitated
R20516	T25761	T25767	ccomp	immunoprecipitated,separated
R20517	T25762	T25761	prep	with,immunoprecipitated
R20518	T25763	T25764	amod	anti-FLAG,antibody
R20519	T25764	T25762	pobj	antibody,with
R20520	T25765	T25767	nsubjpass	immunocomplexes,separated
R20521	T25766	T25767	auxpass	were,separated
R20522	T25767	T25767	ROOT	separated,separated
R20523	T25768	T25767	prep	by,separated
R20524	T25769	T25770	nummod	12,%
R20525	T25770	T25768	pobj	%,by
R20526	T25771	T25772	compound	SDS,PAGE
R20527	T25772	T25767	dobj	PAGE,separated
R20528	T25773	T25772	cc	and,PAGE
R20529	T25774	T25772	conj	analysed,PAGE
R20530	T25775	T25774	agent	by,analysed
R20531	T25776	T25777	amod	western,blotting
R20532	T25777	T25775	pobj	blotting,by
R20533	T25778	T25777	prep	with,blotting
R20534	T25779	T25782	amod	anti-HA,antibodies
R20535	T25780	T25779	cc	and,anti-HA
R20536	T25781	T25779	conj	anti-FLAG,anti-HA
R20537	T25782	T25778	pobj	antibodies,with
R20538	T25783	T25767	punct	.,separated
R20539	T25784	T25785	punct	(,C
R20540	T25785	T25788	nmod	C,p65
R20541	T25786	T25785	punct	),C
R20542	T25787	T25788	amod	35S-methionine-labeled,p65
R20543	T25788	T25794	nsubjpass	p65,incubated
R20544	T25789	T25788	punct	(,p65
R20545	T25790	T25791	nummod	5,µl
R20546	T25791	T25788	appos	µl,p65
R20547	T25792	T25791	punct	),µl
R20548	T25793	T25794	auxpass	was,incubated
R20549	T25794	T25794	ROOT	incubated,incubated
R20550	T25795	T25794	prep	with,incubated
R20551	T25796	T25798	prep	in,translated
R20552	T25797	T25798	nsubj	vitro,translated
R20553	T25798	T25794	conj	translated,incubated
R20554	T25799	T25798	dobj	HA-IκB-α,translated
R20555	T25800	T25802	punct	(,µl
R20556	T25801	T25802	nummod	5,µl
R20557	T25802	T25799	appos	µl,HA-IκB-α
R20558	T25803	T25802	punct	),µl
R20559	T25804	T25798	prep	in,translated
R20560	T25805	T25804	pobj	presence,in
R20561	T25806	T25805	cc	or,presence
R20562	T25807	T25805	conj	absence,presence
R20563	T25808	T25807	prep	of,absence
R20564	T25809	T25808	pobj	FLAG-Tat,of
R20565	T25810	T25809	cc	or,FLAG-Tat
R20566	T25811	T25812	compound	FLAG-Tat,R
R20567	T25812	T25809	conj	R,FLAG-Tat
R20568	T25813	T25809	punct	(,FLAG-Tat
R20569	T25814	T25809	appos	49,FLAG-Tat
R20570	T25815	T25809	appos	57,FLAG-Tat
R20571	T25816	T25809	punct	),FLAG-Tat
R20572	T25817	T25824	det	A,µl
R20573	T25818	T25824	punct	(,µl
R20574	T25819	T25824	nummod	5,µl
R20575	T25820	T25824	punct	",",µl
R20576	T25821	T25824	nummod	10,µl
R20577	T25822	T25821	cc	or,10
R20578	T25823	T25821	conj	20,10
R20579	T25824	T25824	ROOT	µl,µl
R20580	T25825	T25824	punct	),µl
R20581	T25826	T25824	punct	.,µl
R20582	T25827	T25829	nsubjpass	HA-IκB-α,immunoprecipitated
R20583	T25828	T25829	auxpass	was,immunoprecipitated
R20584	T25829	T25836	ccomp	immunoprecipitated,separated
R20585	T25830	T25829	prep	with,immunoprecipitated
R20586	T25831	T25832	amod	anti-HA,antibody
R20587	T25832	T25830	pobj	antibody,with
R20588	T25833	T25836	punct	;,separated
R20589	T25834	T25836	nsubjpass	immunocomplexes,separated
R20590	T25835	T25836	auxpass	were,separated
R20591	T25836	T25836	ROOT	separated,separated
R20592	T25837	T25836	prep	by,separated
R20593	T25838	T25839	nummod	12,%
R20594	T25839	T25840	compound	%,SDS
R20595	T25840	T25837	pobj	SDS,by
R20596	T25841	T25840	conj	PAGE,SDS
R20597	T25842	T25836	cc	and,separated
R20598	T25843	T25836	conj	analysed,separated
R20599	T25844	T25843	agent	by,analysed
R20600	T25845	T25846	amod	western,blotting
R20601	T25846	T25844	pobj	blotting,by
R20602	T25847	T25846	prep	with,blotting
R20603	T25848	T25851	amod	anti-HA,antibodies
R20604	T25849	T25848	cc	and,anti-HA
R20605	T25850	T25848	conj	anti-FLAG,anti-HA
R20606	T25851	T25847	pobj	antibodies,with
R20607	T25852	T25851	punct	",",antibodies
R20608	T25853	T25851	cc	or,antibodies
R20609	T25854	T25851	conj	autoradiography,antibodies
R20610	T25855	T25856	punct	(,35S-Met-p65
R20611	T25856	T25854	appos	35S-Met-p65,autoradiography
R20612	T25857	T25843	punct	),analysed
R20613	T25858	T25836	punct	.,separated
R20614	T25859	T25860	compound	Densitometry,values
R20615	T25860	T25868	nsubjpass	values,expressed
R20616	T25861	T25862	punct	(,D
R20617	T25862	T25860	appos	D,values
R20618	T25863	T25860	punct	),values
R20619	T25864	T25860	prep	of,values
R20620	T25865	T25866	det	the,bands
R20621	T25866	T25864	pobj	bands,of
R20622	T25867	T25868	auxpass	were,expressed
R20623	T25868	T25868	ROOT	expressed,expressed
R20624	T25869	T25870	mark	as,fold
R20625	T25870	T25868	advcl	fold,expressed
R20626	T25871	T25870	dobj	increase,fold
R20627	T25872	T25870	prep	above,fold
R20628	T25873	T25874	det	the,control
R20629	T25874	T25872	pobj	control,above
R20630	T25875	T25874	punct	(,control
R20631	T25876	T25874	appos	lane,control
R20632	T25877	T25876	nummod	1,lane
R20633	T25878	T25874	punct	),control
R20634	T25879	T25868	punct	.,expressed
R20635	T25880	T25881	punct	(,D
R20636	T25881	T25881	ROOT	D,D
R20637	T25882	T25881	punct	),D
R20638	T25883	T25885	det	The,DNA
R20639	T25884	T25885	nummod	p65,DNA
R20640	T25885	T25887	compound	DNA,activity
R20641	T25886	T25887	amod	binding,activity
R20642	T25887	T25889	nsubjpass	activity,analysed
R20643	T25888	T25889	auxpass	was,analysed
R20644	T25889	T25889	ROOT	analysed,analysed
R20645	T25890	T25889	agent	by,analysed
R20646	T25891	T25890	pobj	EMSA,by
R20647	T25892	T25889	advcl	using,analysed
R20648	T25893	T25892	prt	in,using
R20649	T25894	T25896	amod	vitro,proteins
R20650	T25895	T25896	amod	translated,proteins
R20651	T25896	T25893	pobj	proteins,in
R20652	T25897	T25889	punct	.,analysed
R20653	T25898	T25900	amod	32P-labeled-NF-κB,oligonucleotide
R20654	T25899	T25900	amod	double-stranded,oligonucleotide
R20655	T25900	T25902	nsubjpass	oligonucleotide,incubated
R20656	T25901	T25902	auxpass	was,incubated
R20657	T25902	T25902	ROOT	incubated,incubated
R20658	T25903	T25902	prep	with,incubated
R20659	T25904	T25903	pobj	p65,with
R20660	T25905	T25907	punct	(,µl
R20661	T25906	T25907	nummod	0.5,µl
R20662	T25907	T25904	appos	µl,p65
R20663	T25908	T25907	punct	),µl
R20664	T25909	T25907	prep	in,µl
R20665	T25910	T25909	pobj	presence,in
R20666	T25911	T25910	cc	or,presence
R20667	T25912	T25910	conj	absence,presence
R20668	T25913	T25912	prep	of,absence
R20669	T25914	T25923	nmod	HA-IκB-α,R
R20670	T25915	T25917	punct	(,µl
R20671	T25916	T25917	nummod	1,µl
R20672	T25917	T25914	appos	µl,HA-IκB-α
R20673	T25918	T25917	punct	),µl
R20674	T25919	T25917	punct	",",µl
R20675	T25920	T25923	amod	FLAG-Tat,R
R20676	T25921	T25920	cc	or,FLAG-Tat
R20677	T25922	T25920	conj	FLAG-Tat,FLAG-Tat
R20678	T25923	T25913	pobj	R,of
R20679	T25924	T25923	punct	(,R
R20680	T25925	T25923	appos	49,R
R20681	T25926	T25923	appos	57,R
R20682	T25927	T25923	punct	),R
R20683	T25928	T25933	det	A,µl
R20684	T25929	T25930	punct	(,5
R20685	T25930	T25933	nummod	5,µl
R20686	T25931	T25930	cc	and,5
R20687	T25932	T25930	conj	10,5
R20688	T25933	T25941	nsubjpass	µl,performed
R20689	T25934	T25933	punct	),µl
R20690	T25935	T25936	punct	;,competition
R20691	T25936	T25941	nsubjpass	competition,performed
R20692	T25937	T25936	prep	of,competition
R20693	T25938	T25937	pobj	DNA,of
R20694	T25939	T25941	nsubjpass	binding,performed
R20695	T25940	T25941	auxpass	was,performed
R20696	T25941	T25941	ROOT	performed,performed
R20697	T25942	T25941	prep	with,performed
R20698	T25943	T25949	nummod	10,excess
R20699	T25944	T25945	punct	-,up
R20700	T25945	T25949	nmod	up,excess
R20701	T25946	T25947	quantmod	to,100-fold
R20702	T25947	T25949	nmod	100-fold,excess
R20703	T25948	T25949	compound	molar,excess
R20704	T25949	T25942	pobj	excess,with
R20705	T25950	T25949	prep	of,excess
R20706	T25951	T25954	amod	unlabeled,oligonucleotide
R20707	T25952	T25954	amod	NF-κB,oligonucleotide
R20708	T25953	T25954	amod	double-stranded,oligonucleotide
R20709	T25954	T25950	pobj	oligonucleotide,of
R20710	T25955	T25941	punct	.,performed
R20711	T25956	T25957	compound	DNA/protein,complexes
R20712	T25957	T25959	nsubjpass	complexes,run
R20713	T25958	T25959	auxpass	were,run
R20714	T25959	T25959	ROOT	run,run
R20715	T25960	T25959	prep	on,run
R20716	T25961	T25962	nummod	6,%
R20717	T25962	T25963	compound	%,PAGE
R20718	T25963	T25960	pobj	PAGE,on
R20719	T25964	T25963	appos	TBE,PAGE
R20720	T25965	T25959	cc	and,run
R20721	T25966	T25959	conj	analysed,run
R20722	T25967	T25966	agent	by,analysed
R20723	T25968	T25967	pobj	autoradiography,by
R20724	T25969	T25959	punct	.,run
R20725	T25970	T25989	punct	(,transfected
R20726	T25971	T25980	dep	E,−
R20727	T25972	T25973	punct	),p50
R20728	T25973	T25980	dep	p50,−
R20729	T25974	T25980	dep	−,−
R20730	T25975	T25980	dep	/,−
R20731	T25976	T25980	dep	−,−
R20732	T25977	T25980	dep	p65,−
R20733	T25978	T25980	dep	−,−
R20734	T25979	T25980	dep	/,−
R20735	T25980	T25981	nmod	−,MEFs
R20736	T25981	T25989	nsubjpass	MEFs,transfected
R20737	T25982	T25981	punct	(,MEFs
R20738	T25983	T25986	nummod	3,cells
R20739	T25984	T25986	nummod	×,cells
R20740	T25985	T25986	nummod	105,cells
R20741	T25986	T25981	appos	cells,MEFs
R20742	T25987	T25981	punct	),MEFs
R20743	T25988	T25989	auxpass	were,transfected
R20744	T25989	T25989	ROOT	transfected,transfected
R20745	T25990	T25989	prep	with,transfected
R20746	T25991	T25990	pobj	pκBLuc,with
R20747	T25992	T25994	punct	(,µg
R20748	T25993	T25994	nummod	0.5,µg
R20749	T25994	T25991	appos	µg,pκBLuc
R20750	T25995	T25994	punct	),µg
R20751	T25996	T25991	cc	and,pκBLuc
R20752	T25997	T25991	conj	pSV-β-Gal,pκBLuc
R20753	T25998	T26000	punct	(,µg
R20754	T25999	T26000	nummod	0.1,µg
R20755	T26000	T25997	appos	µg,pSV-β-Gal
R20756	T26001	T26000	punct	),µg
R20757	T26002	T25997	prep	with,pSV-β-Gal
R20758	T26003	T26002	cc	or,with
R20759	T26004	T26002	conj	without,with
R20760	T26005	T26004	pobj	pRc/CMV-p,without
R20761	T26006	T26005	nummod	65,pRc/CMV-p
R20762	T26007	T26009	punct	(,µg
R20763	T26008	T26009	nummod	0.5,µg
R20764	T26009	T26005	appos	µg,pRc/CMV-p
R20765	T26010	T26009	punct	),µg
R20766	T26011	T26005	punct	",",pRc/CMV-p
R20767	T26012	T26005	appos	pCMV4-HA-IκB-α,pRc/CMV-p
R20768	T26013	T26015	punct	(,µg
R20769	T26014	T26015	nummod	0.5,µg
R20770	T26015	T26012	appos	µg,pCMV4-HA-IκB-α
R20771	T26016	T26015	punct	),µg
R20772	T26017	T26015	punct	",",µg
R20773	T26018	T26015	conj	p3xFLAG-Tat,µg
R20774	T26019	T26018	cc	or,p3xFLAG-Tat
R20775	T26020	T26018	conj	p3xFLAG-Tat,p3xFLAG-Tat
R20776	T26021	T26004	pobj	R,without
R20777	T26022	T26024	punct	(,57
R20778	T26023	T26024	nummod	49,57
R20779	T26024	T26021	appos	57,R
R20780	T26025	T26021	punct	),R
R20781	T26026	T26033	det	A,µg
R20782	T26027	T26033	punct	(,µg
R20783	T26028	T26033	nummod	0.5,µg
R20784	T26029	T26030	punct	",",1
R20785	T26030	T26028	prep	1,0.5
R20786	T26031	T26028	cc	and,0.5
R20787	T26032	T26028	conj	2,0.5
R20788	T26033	T26033	ROOT	µg,µg
R20789	T26034	T26033	punct	),µg
R20790	T26035	T26033	punct	.,µg
R20791	T26036	T26038	det	The,activity
R20792	T26037	T26038	compound	luciferase,activity
R20793	T26038	T26040	nsubjpass	activity,measured
R20794	T26039	T26040	auxpass	was,measured
R20795	T26040	T26040	ROOT	measured,measured
R20796	T26041	T26040	prep	in,measured
R20797	T26042	T26043	compound	cell,extracts
R20798	T26043	T26041	pobj	extracts,in
R20799	T26044	T26045	compound	48-h,post-transfection
R20800	T26045	T26043	appos	post-transfection,extracts
R20801	T26046	T26045	cc	and,post-transfection
R20802	T26047	T26045	conj	normalized,post-transfection
R20803	T26048	T26047	prep	to,normalized
R20804	T26049	T26050	amod	β-galactosidase,activity
R20805	T26050	T26048	pobj	activity,to
R20806	T26051	T26040	punct	.,measured
R20807	T26052	T26053	compound	Fold,activation
R20808	T26053	T26055	nsubjpass	activation,calculated
R20809	T26054	T26055	auxpass	was,calculated
R20810	T26055	T26055	ROOT	calculated,calculated
R20811	T26056	T26055	oprd	relative,calculated
R20812	T26057	T26056	prep	to,relative
R20813	T26058	T26057	pobj	transfection,to
R20814	T26059	T26058	prep	of,transfection
R20815	T26060	T26062	det	the,plasmid
R20816	T26061	T26062	compound	pκBLuc,plasmid
R20817	T26062	T26059	pobj	plasmid,of
R20818	T26063	T26058	advmod	alone,transfection
R20819	T26064	T26055	punct	.,calculated
R20820	T26065	T26076	nsubjpass	Values,shown
R20821	T26066	T26069	punct	(,SE
R20822	T26067	T26069	amod	mean,SE
R20823	T26068	T26069	compound	±,SE
R20824	T26069	T26065	appos	SE,Values
R20825	T26070	T26069	punct	",",SE
R20826	T26071	T26073	nmod	n,3
R20827	T26072	T26073	punct	=,3
R20828	T26073	T26069	appos	3,SE
R20829	T26074	T26069	punct	),SE
R20830	T26075	T26076	auxpass	are,shown
R20831	T26076	T26076	ROOT	shown,shown
R20832	T26077	T26076	punct	.,shown
R20833	T26078	T26093	prep	As,analysed
R20834	T26079	T26078	pobj	control,As
R20835	T26080	T26079	prep	of,control
R20836	T26081	T26082	compound	protein,expression
R20837	T26082	T26080	pobj	expression,of
R20838	T26083	T26093	punct	",",analysed
R20839	T26084	T26093	nsubjpass	aliquots,analysed
R20840	T26085	T26084	prep	of,aliquots
R20841	T26086	T26087	compound	cell,extracts
R20842	T26087	T26085	pobj	extracts,of
R20843	T26088	T26087	punct	(,extracts
R20844	T26089	T26090	nummod	20,µg
R20845	T26090	T26087	appos	µg,extracts
R20846	T26091	T26087	punct	),extracts
R20847	T26092	T26093	auxpass	were,analysed
R20848	T26093	T26093	ROOT	analysed,analysed
R20849	T26094	T26093	agent	by,analysed
R20850	T26095	T26096	amod	western,blotting
R20851	T26096	T26094	pobj	blotting,by
R20852	T26097	T26096	prep	with,blotting
R20853	T26098	T26105	amod	anti-p65,antibodies
R20854	T26099	T26098	punct	",",anti-p65
R20855	T26100	T26105	amod	anti-HA,antibodies
R20856	T26101	T26100	punct	",",anti-HA
R20857	T26102	T26100	conj	anti-FLAG,anti-HA
R20858	T26103	T26102	cc	and,anti-FLAG
R20859	T26104	T26102	conj	anti-γ-tubulin,anti-FLAG
R20860	T26105	T26097	pobj	antibodies,with
R20861	T26106	T26093	punct	.,analysed

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T12463	161-171	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated
T12464	161-180	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated proteins
T12465	3424-3439	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptional
T12466	3867-3882	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptional
T26107	1194-1204	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated
T26108	1483-1493	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated
T26109	1194-1213	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated proteins
T26110	1959-1978	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated proteins
T26111	2582-2601	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T26112	2582-2601	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T26113	2582-2601	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T26114	2582-2601	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T26115	2582-2601	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T26116	2673-2697	http://purl.obolibrary.org/obo/GO_0004565	denotes	β-galactosidase activity
T26117	2673-2697	http://purl.obolibrary.org/obo/GO_0004336	denotes	β-galactosidase activity
T26118	2673-2697	http://purl.obolibrary.org/obo/GO_1903771	denotes	β-galactosidase activity
T26119	2673-2697	http://purl.obolibrary.org/obo/GO_1903770	denotes	β-galactosidase activity
T26120	2673-2697	http://purl.obolibrary.org/obo/GO_0033920	denotes	β-galactosidase activity
T26121	2673-2697	http://purl.obolibrary.org/obo/GO_0033929	denotes	β-galactosidase activity
T26122	2673-2697	http://purl.obolibrary.org/obo/GO_0033930	denotes	β-galactosidase activity

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T12467	71-78	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12468	531-538	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12469	656-663	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12470	769-776	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12471	846-853	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12472	3069-3076	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12473	3222-3229	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12474	3855-3862	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12475	3946-3953	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T12476	3069-3083	http://purl.obolibrary.org/obo/GO_0003677	denotes	binding to DNA
T12477	3218-3229	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T12478	3851-3862	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA-binding
T26123	1288-1296	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T26124	1630-1638	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T26125	1747-1757	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T26126	2976-2986	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T26127	1902-1913	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T26128	2166-2177	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T26129	1906-1913	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T26130	2170-2177	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T26131	2582-2601	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T26132	2582-2601	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T26133	2582-2601	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T26134	2582-2601	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T26135	2582-2601	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T26136	2673-2697	http://purl.obolibrary.org/obo/GO_0004565	denotes	β-galactosidase activity
T26137	2673-2697	http://purl.obolibrary.org/obo/GO_0004336	denotes	β-galactosidase activity
T26138	2673-2697	http://purl.obolibrary.org/obo/GO_0033920	denotes	β-galactosidase activity
T26139	2673-2697	http://purl.obolibrary.org/obo/GO_0033929	denotes	β-galactosidase activity
T26140	2673-2697	http://purl.obolibrary.org/obo/GO_0033930	denotes	β-galactosidase activity

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T26141	1288-1296	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T26142	1630-1638	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T26143	1747-1757	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T26144	2976-2986	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T26145	1288-1296	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T26146	1630-1638	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T26147	1747-1757	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T26148	2976-2986	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T26149	2282-2303	http://purl.obolibrary.org/obo/GO_0001114	denotes	DNA/protein complexes
T26150	2282-2303	http://purl.obolibrary.org/obo/GO_0032993	denotes	DNA/protein complexes
T26151	2282-2303	http://purl.obolibrary.org/obo/GO_0097522	denotes	DNA/protein complexes
T26152	2286-2303	http://purl.obolibrary.org/obo/GO_0043234	denotes	protein complexes
T26153	2391-2396	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T26154	2618-2622	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T26155	2867-2871	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell

sentences

Id	Subject	Object	Predicate	Lexical cue
T11602	0-78	Sentence	denotes	Tat counteracts the IκB-α inhibition of p65 by competing the repressor binding
T11603	79-213	Sentence	denotes	We further investigated the physical interaction of Tat with IκB-α using in vitro translated proteins in coimmunoprecipitation assays.
T11604	214-285	Sentence	denotes	For mapping the interaction domains, we used the following Tat mutants:
T11605	286-365	Sentence	denotes	Tat T,N(23,24)A, Tat C(22,25,27)A, Tat K(50,51)A and Tat R(49–57)A (Figure 3A).
T11606	366-449	Sentence	denotes	IκB-α coimmunoprecipitated with all Tat proteins, except Tat R(49–57)A (Figure 3B).
T11607	450-605	Sentence	denotes	These results indicated that the arginine-rich domain of Tat was involved in the binding to IκB-α, which was consistent with previous observations (50,51).
T11608	606-893	Sentence	denotes	Moreover, Tat associating with IκB-α competed the binding of IκB-α to p65 in a dose-dependent manner (Figure 3C, lanes 3–5), while Tat R(49–57)A, which lacked the binding site for IκB-α, did not associate with IκB-α and did not compete the binding of IκB-α to p65 (Figure 3C, lanes 6–8).
T11609	2988-3209	Sentence	denotes	Next, we evaluated whether Tat counteracted the IκB-α-mediated inhibition of p65 binding to DNA by incubating in vitro translated p65 and IκB-α proteins with the NF-κB probe in presence or absence of Tat followed by EMSA.
T11610	3210-3356	Sentence	denotes	The p65 DNA binding activity was inhibited by IκB-α and restored in a dose-dependent manner by wild-type Tat, and not by Tat R(49–57) (Figure 3D).
T11611	3357-3762	Sentence	denotes	Further, we analysed the Tat effect on the IκB-α repression of p65 transcriptional activity by transfecting p50−/−p65−/−MEFs with the NF-κB-Luc reporter together with expression vectors of p65 and IκB-α, in presence or absence of Tat. IκB-α inhibited the p65-dependent expression of the luciferase gene, which was restored in a dose-dependent manner by wild-type Tat, and not by Tat R(49–57)A (Figure 3E).
T11612	3763-3961	Sentence	denotes	Altogether these results indicated that Tat counteracts the IκB-α repression of the p65 DNA-binding and transcriptional activity by associating with IκB-α and competing the repressor binding to p65.
T25204	904-1214	Sentence	denotes	Tat relieves p65 from the IκB-α inhibition. (A) Schematic representation of wild-type and mutant Tat proteins. (B) HA-IκB-α (5 µl) was incubated in presence or absence of FLAG-Tat, FLAG-Tat T,N(23,24)A, FLAG-Tat K(50,51)A, FLAG-Tat R(49–57)A or FLAG-Tat C(22,25,27)A (10 µl) using in vitro translated proteins.
T25205	1215-1584	Sentence	denotes	Wild-type and mutant Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies. (C) 35S-methionine-labeled p65 (5 µl) was incubated with in vitro translated HA-IκB-α (5 µl) in presence or absence of FLAG-Tat or FLAG-Tat R(49–57)A (5, 10 or 20 µl).
T25206	1585-1792	Sentence	denotes	HA-IκB-α was immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies, or autoradiography (35S-Met-p65).
T25207	1793-1979	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1). (D) The p65 DNA binding activity was analysed by EMSA using in vitro translated proteins.
T25208	1980-2281	Sentence	denotes	32P-labeled-NF-κB double-stranded oligonucleotide was incubated with p65 (0.5 µl) in presence or absence of HA-IκB-α (1 µl), FLAG-Tat or FLAG-Tat R(49–57)A (5 and 10 µl); competition of DNA binding was performed with 10 - up to 100-fold molar excess of unlabeled NF-κB double-stranded oligonucleotide.
T25209	2282-2577	Sentence	denotes	DNA/protein complexes were run on 6% PAGE–TBE and analysed by autoradiography. (E) p50−/−p65−/−MEFs (3 × 105 cells) were transfected with pκBLuc (0.5 µg) and pSV-β-Gal (0.1 µg) with or without pRc/CMV-p65 (0.5 µg), pCMV4-HA-IκB-α (0.5 µg), p3xFLAG-Tat or p3xFLAG-Tat R(49–57)A (0.5, 1 and 2 µg).
T25210	2578-2698	Sentence	denotes	The luciferase activity was measured in cell extracts 48-h post-transfection and normalized to β-galactosidase activity.
T25211	2699-2783	Sentence	denotes	Fold activation was calculated relative to transfection of the pκBLuc plasmid alone.
T25212	2784-2820	Sentence	denotes	Values (mean ± SE, n = 3) are shown.
T25213	2821-2987	Sentence	denotes	As control of protein expression, aliquots of cell extracts (20 µg) were analysed by western blotting with anti-p65, anti-HA, anti-FLAG and anti-γ-tubulin antibodies.
T142	0-78	Sentence	denotes	Tat counteracts the IκB-α inhibition of p65 by competing the repressor binding
T143	79-213	Sentence	denotes	We further investigated the physical interaction of Tat with IκB-α using in vitro translated proteins in coimmunoprecipitation assays.
T144	214-285	Sentence	denotes	For mapping the interaction domains, we used the following Tat mutants:
T145	286-365	Sentence	denotes	Tat T,N(23,24)A, Tat C(22,25,27)A, Tat K(50,51)A and Tat R(49–57)A (Figure 3A).
T146	366-449	Sentence	denotes	IκB-α coimmunoprecipitated with all Tat proteins, except Tat R(49–57)A (Figure 3B).
T147	450-605	Sentence	denotes	These results indicated that the arginine-rich domain of Tat was involved in the binding to IκB-α, which was consistent with previous observations (50,51).
T148	606-893	Sentence	denotes	Moreover, Tat associating with IκB-α competed the binding of IκB-α to p65 in a dose-dependent manner (Figure 3C, lanes 3–5), while Tat R(49–57)A, which lacked the binding site for IκB-α, did not associate with IκB-α and did not compete the binding of IκB-α to p65 (Figure 3C, lanes 6–8).
T149	894-903	Sentence	denotes	Figure 3.
T150	904-1214	Sentence	denotes	Tat relieves p65 from the IκB-α inhibition. (A) Schematic representation of wild-type and mutant Tat proteins. (B) HA-IκB-α (5 µl) was incubated in presence or absence of FLAG-Tat, FLAG-Tat T,N(23,24)A, FLAG-Tat K(50,51)A, FLAG-Tat R(49–57)A or FLAG-Tat C(22,25,27)A (10 µl) using in vitro translated proteins.
T151	1215-1584	Sentence	denotes	Wild-type and mutant Tat proteins were immunoprecipitated with anti-FLAG antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies. (C) 35S-methionine-labeled p65 (5 µl) was incubated with in vitro translated HA-IκB-α (5 µl) in presence or absence of FLAG-Tat or FLAG-Tat R(49–57)A (5, 10 or 20 µl).
T152	1585-1792	Sentence	denotes	HA-IκB-α was immunoprecipitated with anti-HA antibody; immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-HA and anti-FLAG antibodies, or autoradiography (35S-Met-p65).
T153	1793-1979	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1). (D) The p65 DNA binding activity was analysed by EMSA using in vitro translated proteins.
T154	1980-2281	Sentence	denotes	32P-labeled-NF-κB double-stranded oligonucleotide was incubated with p65 (0.5 µl) in presence or absence of HA-IκB-α (1 µl), FLAG-Tat or FLAG-Tat R(49–57)A (5 and 10 µl); competition of DNA binding was performed with 10 - up to 100-fold molar excess of unlabeled NF-κB double-stranded oligonucleotide.
T155	2282-2577	Sentence	denotes	DNA/protein complexes were run on 6% PAGE–TBE and analysed by autoradiography. (E) p50−/−p65−/−MEFs (3 × 105 cells) were transfected with pκBLuc (0.5 µg) and pSV-β-Gal (0.1 µg) with or without pRc/CMV-p65 (0.5 µg), pCMV4-HA-IκB-α (0.5 µg), p3xFLAG-Tat or p3xFLAG-Tat R(49–57)A (0.5, 1 and 2 µg).
T156	2578-2698	Sentence	denotes	The luciferase activity was measured in cell extracts 48-h post-transfection and normalized to β-galactosidase activity.
T157	2699-2783	Sentence	denotes	Fold activation was calculated relative to transfection of the pκBLuc plasmid alone.
T158	2784-2820	Sentence	denotes	Values (mean ± SE, n = 3) are shown.
T159	2821-2987	Sentence	denotes	As control of protein expression, aliquots of cell extracts (20 µg) were analysed by western blotting with anti-p65, anti-HA, anti-FLAG and anti-γ-tubulin antibodies.
T160	2988-3209	Sentence	denotes	Next, we evaluated whether Tat counteracted the IκB-α-mediated inhibition of p65 binding to DNA by incubating in vitro translated p65 and IκB-α proteins with the NF-κB probe in presence or absence of Tat followed by EMSA.
T161	3210-3356	Sentence	denotes	The p65 DNA binding activity was inhibited by IκB-α and restored in a dose-dependent manner by wild-type Tat, and not by Tat R(49–57) (Figure 3D).
T162	3357-3591	Sentence	denotes	Further, we analysed the Tat effect on the IκB-α repression of p65 transcriptional activity by transfecting p50−/−p65−/−MEFs with the NF-κB-Luc reporter together with expression vectors of p65 and IκB-α, in presence or absence of Tat.
T163	3592-3762	Sentence	denotes	IκB-α inhibited the p65-dependent expression of the luciferase gene, which was restored in a dose-dependent manner by wild-type Tat, and not by Tat R(49–57)A (Figure 3E).
T164	3763-3961	Sentence	denotes	Altogether these results indicated that Tat counteracts the IκB-α repression of the p65 DNA-binding and transcriptional activity by associating with IκB-α and competing the repressor binding to p65.

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T12909	0-3	Protein	denotes	Tat
T12910	4-15	Negative_regulation	denotes	counteracts
T12911	20-25	Protein	denotes	IκB-α
T12912	26-36	Negative_regulation	denotes	inhibition
T12913	40-43	Protein	denotes	p65
T12914	47-56	Negative_regulation	denotes	competing
T12915	71-78	Binding	denotes	binding
T12916	116-127	Binding	denotes	interaction
T12917	131-134	Protein	denotes	Tat
T12918	140-145	Protein	denotes	IκB-α
T12919	273-276	Protein	denotes	Tat
T12920	286-301	Protein	denotes	Tat T,N(23,24)A
T12921	303-319	Protein	denotes	Tat C(22,25,27)A
T12922	321-334	Protein	denotes	Tat K(50,51)A
T12923	339-352	Protein	denotes	Tat R(49–57)A
T12924	366-371	Protein	denotes	IκB-α
T12925	372-392	Binding	denotes	coimmunoprecipitated	true
T12926	372-392	Binding	denotes	coimmunoprecipitated	true
T12927	402-405	Protein	denotes	Tat
T12928	423-436	Protein	denotes	Tat R(49–57)A
T12929	483-503	Entity	denotes	arginine-rich domain
T12930	507-510	Protein	denotes	Tat
T12931	515-523	Positive_regulation	denotes	involved
T12932	531-538	Binding	denotes	binding
T12933	542-547	Protein	denotes	IκB-α
T12934	616-619	Protein	denotes	Tat
T12935	620-631	Binding	denotes	associating
T12936	637-642	Protein	denotes	IκB-α
T12937	643-651	Negative_regulation	denotes	competed
T12938	656-663	Binding	denotes	binding
T12939	667-672	Protein	denotes	IκB-α
T12940	676-679	Protein	denotes	p65
T12941	737-750	Protein	denotes	Tat R(49–57)A
T12942	758-764	Negative_regulation	denotes	lacked
T12943	769-776	Binding	denotes	binding
T12944	777-781	Entity	denotes	site
T12945	786-791	Protein	denotes	IκB-α
T12946	801-810	Binding	denotes	associate	true
T12947	816-821	Protein	denotes	IκB-α
T12948	834-841	Negative_regulation	denotes	compete	true
T12949	846-853	Binding	denotes	binding
T12950	857-862	Protein	denotes	IκB-α
T12951	866-869	Protein	denotes	p65
T12952	3015-3018	Protein	denotes	Tat
T12953	3019-3031	Negative_regulation	denotes	counteracted		true
T12954	3036-3041	Protein	denotes	IκB-α
T12955	3051-3061	Negative_regulation	denotes	inhibition
T12956	3065-3068	Protein	denotes	p65
T12957	3069-3076	Binding	denotes	binding
T12958	3118-3121	Protein	denotes	p65
T12959	3126-3131	Protein	denotes	IκB-α
T12960	3188-3191	Protein	denotes	Tat
T12961	3214-3217	Protein	denotes	p65
T12962	3222-3229	Binding	denotes	binding
T12963	3243-3252	Negative_regulation	denotes	inhibited
T12964	3256-3261	Protein	denotes	IκB-α
T12965	3266-3274	Negative_regulation	denotes	restored	true
T12966	3266-3274	Negative_regulation	denotes	restored	true
T12967	3315-3318	Protein	denotes	Tat
T12968	3331-3343	Protein	denotes	Tat R(49–57)
T12969	3382-3385	Protein	denotes	Tat
T12970	3400-3405	Protein	denotes	IκB-α
T12971	3420-3423	Protein	denotes	p65
T12972	3465-3468	Protein	denotes	p50
T12973	3471-3474	Protein	denotes	p65
T12974	3497-3500	Protein	denotes	Luc
T12975	3546-3549	Protein	denotes	p65
T12976	3554-3559	Protein	denotes	IκB-α
T12977	3587-3590	Protein	denotes	Tat
T12978	3592-3597	Protein	denotes	IκB-α
T12979	3598-3607	Negative_regulation	denotes	inhibited
T12980	3612-3615	Protein	denotes	p65
T12981	3616-3625	Positive_regulation	denotes	dependent
T12982	3626-3636	Gene_expression	denotes	expression
T12983	3644-3654	Protein	denotes	luciferase
T12984	3671-3679	Negative_regulation	denotes	restored	true
T12985	3671-3679	Negative_regulation	denotes	restored	true
T12986	3720-3723	Protein	denotes	Tat
T12987	3736-3749	Protein	denotes	Tat R(49–57)A
T12988	3803-3806	Protein	denotes	Tat
T12989	3807-3818	Negative_regulation	denotes	counteracts
T12990	3807-3818	Negative_regulation	denotes	counteracts
T12991	3823-3828	Protein	denotes	IκB-α
T12992	3829-3839	Negative_regulation	denotes	repression
T12993	3847-3850	Protein	denotes	p65
T12994	3855-3862	Binding	denotes	binding
T12995	3895-3906	Binding	denotes	associating
T12996	3912-3917	Protein	denotes	IκB-α
T12997	3922-3931	Negative_regulation	denotes	competing
T12998	3946-3953	Binding	denotes	binding
T12999	3957-3960	Protein	denotes	p65
T26333	904-907	Protein	denotes	Tat
T26334	908-916	Negative_regulation	denotes	relieves
T26335	917-920	Protein	denotes	p65
T26336	930-935	Protein	denotes	IκB-α
T26337	936-946	Negative_regulation	denotes	inhibition
T26338	1001-1004	Protein	denotes	Tat
T26339	1019-1027	Protein	denotes	HA-IκB-α
T26340	1075-1083	Protein	denotes	FLAG-Tat
T26341	1085-1105	Protein	denotes	FLAG-Tat T,N(23,24)A
T26342	1107-1125	Protein	denotes	FLAG-Tat K(50,51)A
T26343	1127-1145	Protein	denotes	FLAG-Tat R(49–57)A
T26344	1149-1170	Protein	denotes	FLAG-Tat C(22,25,27)A
T26345	1236-1239	Protein	denotes	Tat
T26346	1444-1447	Protein	denotes	p65
T26347	1494-1502	Protein	denotes	HA-IκB-α
T26348	1536-1544	Protein	denotes	FLAG-Tat
T26349	1548-1566	Protein	denotes	FLAG-Tat R(49–57)A
T26350	1585-1593	Protein	denotes	HA-IκB-α
T26351	1787-1790	Protein	denotes	p65
T26352	1898-1901	Protein	denotes	p65
T26353	1906-1913	Binding	denotes	binding		true
T26354	2049-2052	Protein	denotes	p65
T26355	2088-2096	Protein	denotes	HA-IκB-α
T26356	2105-2113	Protein	denotes	FLAG-Tat
T26357	2117-2135	Protein	denotes	FLAG-Tat R(49–57)A
T26358	2151-2162	Negative_regulation	denotes	competition		true
T26359	2170-2177	Binding	denotes	binding
T26360	2365-2368	Protein	denotes	p50
T26361	2371-2374	Protein	denotes	p65
T26362	2444-2449	Protein	denotes	β-Gal
T26363	2483-2486	Protein	denotes	p65
T26364	2503-2511	Protein	denotes	HA-IκB-α
T26365	2530-2533	Protein	denotes	Tat
T26366	2545-2548	Protein	denotes	Tat
T26367	2582-2592	Protein	denotes	luciferase
T26368	2673-2688	Protein	denotes	β-galactosidase
R10487	T12909	T12914	causeOf	Tat,competing
R10488	T12911	T12915	themeOf	IκB-α,binding
R10489	T12911	T12912	causeOf	IκB-α,inhibition
R10490	T12912	T12910	themeOf	inhibition,counteracts
R10491	T12913	T12912	themeOf	p65,inhibition
R10492	T12914	T12910	causeOf	competing,counteracts
R10493	T12915	T12914	themeOf	binding,competing
R10494	T12917	T12916	themeOf	Tat,interaction
R10495	T12918	T12916	themeOf	IκB-α,interaction
R10496	T12924	T12926	themeOf	IκB-α,coimmunoprecipitated
R10497	T12924	T12925	themeOf	IκB-α,coimmunoprecipitated
R10498	T12927	T12926	themeOf	Tat,coimmunoprecipitated
R10499	T12928	T12925	themeOf	Tat R(49–57)A,coimmunoprecipitated
R10500	T12929	T12931	causeOf	arginine-rich domain,involved
R10501	T12929	T12930	partOf	arginine-rich domain,Tat
R10502	T12930	T12932	themeOf	Tat,binding
R10503	T12932	T12931	themeOf	binding,involved
R10504	T12933	T12932	themeOf	IκB-α,binding
R10505	T12934	T12935	themeOf	Tat,associating
R10506	T12935	T12937	causeOf	associating,competed
R10507	T12936	T12935	themeOf	IκB-α,associating
R10508	T12938	T12937	themeOf	binding,competed
R10509	T12939	T12938	themeOf	IκB-α,binding
R10510	T12940	T12938	themeOf	p65,binding
R10511	T12941	T12946	themeOf	Tat R(49–57)A,associate
R10512	T12941	T12948	causeOf	Tat R(49–57)A,compete
R10513	T12944	T12942	themeOf	site,lacked
R10514	T12944	T12934	partOf	site,Tat
R10515	T12944	T12943	themeOf	site,binding
R10516	T12945	T12943	themeOf	IκB-α,binding
R10517	T12947	T12946	themeOf	IκB-α,associate
R10518	T12949	T12948	themeOf	binding,compete
R10519	T12950	T12949	themeOf	IκB-α,binding
R10520	T12951	T12949	themeOf	p65,binding
R10521	T12952	T12953	causeOf	Tat,counteracted
R10522	T12954	T12955	causeOf	IκB-α,inhibition
R10523	T12955	T12953	themeOf	inhibition,counteracted
R10524	T12956	T12957	themeOf	p65,binding
R10525	T12957	T12955	themeOf	binding,inhibition
R10526	T12961	T12962	themeOf	p65,binding
R10527	T12962	T12963	themeOf	binding,inhibited
R10528	T12963	T12966	themeOf	inhibited,restored
R10529	T12963	T12965	themeOf	inhibited,restored
R10530	T12964	T12963	causeOf	IκB-α,inhibited
R10531	T12967	T12966	causeOf	Tat,restored
R10532	T12968	T12965	causeOf	Tat R(49–57),restored
R10533	T12978	T12979	causeOf	IκB-α,inhibited
R10534	T12979	T12984	themeOf	inhibited,restored
R10535	T12979	T12985	themeOf	inhibited,restored
R10536	T12980	T12981	causeOf	p65,dependent
R10537	T12981	T12979	themeOf	dependent,inhibited
R10538	T12982	T12981	themeOf	expression,dependent
R10539	T12983	T12982	themeOf	luciferase,expression
R10540	T12986	T12984	causeOf	Tat,restored
R10541	T12987	T12985	causeOf	Tat R(49–57)A,restored
R10542	T12988	T12995	themeOf	Tat,associating
R10543	T12988	T12997	causeOf	Tat,competing
R10544	T12991	T12992	causeOf	IκB-α,repression
R10545	T12992	T12989	themeOf	repression,counteracts
R10546	T12992	T12990	themeOf	repression,counteracts
R10547	T12993	T12994	themeOf	p65,binding
R10548	T12994	T12992	themeOf	binding,repression
R10549	T12995	T12989	causeOf	associating,counteracts
R10550	T12996	T12995	themeOf	IκB-α,associating
R10551	T12996	T12998	themeOf	IκB-α,binding
R10552	T12997	T12990	causeOf	competing,counteracts
R10553	T12998	T12997	themeOf	binding,competing
R10554	T12999	T12998	themeOf	p65,binding
R20896	T26333	T26334	causeOf	Tat,relieves
R20897	T26335	T26337	themeOf	p65,inhibition
R20898	T26336	T26337	causeOf	IκB-α,inhibition
R20899	T26337	T26334	themeOf	inhibition,relieves
R20900	T26352	T26353	themeOf	p65,binding
R20901	T26354	T26359	themeOf	p65,binding
R20902	T26355	T26358	causeOf	HA-IκB-α,competition
R20903	T26359	T26358	themeOf	binding,competition

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T13000	0-3	Protein	denotes	Tat
T13001	20-25	Protein	denotes	IκB-α
T13002	40-43	Protein	denotes	p65
T13003	4-15	Binding	denotes	counteracts
T13004	26-36	Negative_regulation	denotes	inhibition
T13005	26-36	Negative_regulation	denotes	inhibition
T13006	131-134	Protein	denotes	Tat
T13007	140-145	Protein	denotes	IκB-α
T13008	116-127	Binding	denotes	interaction
T13009	273-284	Protein	denotes	Tat mutants
T13010	366-371	Protein	denotes	IκB-α
T13011	402-414	Protein	denotes	Tat proteins
T13012	423-426	Protein	denotes	Tat
T13013	507-510	Protein	denotes	Tat
T13014	542-547	Protein	denotes	IκB-α
T13015	531-538	Binding	denotes	binding
T13016	616-619	Protein	denotes	Tat
T13017	637-642	Protein	denotes	IκB-α
T13018	667-672	Protein	denotes	IκB-α
T13019	676-679	Protein	denotes	p65
T13020	737-742	Protein	denotes	Tat R
T13021	786-791	Protein	denotes	IκB-α
T13022	816-821	Protein	denotes	IκB-α
T13023	857-862	Protein	denotes	IκB-α
T13024	620-631	Binding	denotes	associating
T13025	656-663	Binding	denotes	binding
T13026	769-776	Binding	denotes	binding
T13027	801-810	Binding	denotes	associate
T13028	801-810	Binding	denotes	associate
T13029	846-853	Binding	denotes	binding
T13030	643-651	Negative_regulation	denotes	competed
T13031	758-764	Negative_regulation	denotes	lacked
T13032	834-841	Negative_regulation	denotes	compete
T13033	3015-3018	Protein	denotes	Tat
T13034	3036-3041	Protein	denotes	IκB-α
T13035	3065-3068	Protein	denotes	p65
T13036	3118-3121	Protein	denotes	p65
T13037	3126-3140	Protein	denotes	IκB-α proteins
T13038	3150-3155	Protein	denotes	NF-κB
T13039	3188-3191	Protein	denotes	Tat
T13040	3019-3031	Binding	denotes	counteracted
T13041	3069-3076	Binding	denotes	binding
T13042	3051-3061	Negative_regulation	denotes	inhibition
T13043	3214-3221	Protein	denotes	p65 DNA
T13044	3256-3261	Protein	denotes	IκB-α
T13045	3331-3336	Protein	denotes	Tat R
T13046	3222-3229	Binding	denotes	binding
T13047	3243-3252	Negative_regulation	denotes	inhibited
T13048	3266-3274	Positive_regulation	denotes	restored
T13049	3266-3274	Positive_regulation	denotes	restored
T13050	3382-3385	Protein	denotes	Tat
T13051	3400-3405	Protein	denotes	IκB-α
T13052	3420-3423	Protein	denotes	p65
T13053	3491-3496	Protein	denotes	NF-κB
T13054	3497-3500	Protein	denotes	Luc
T13055	3546-3549	Protein	denotes	p65
T13056	3554-3559	Protein	denotes	IκB-α
T13057	3587-3590	Protein	denotes	Tat
T13058	3406-3416	Negative_regulation	denotes	repression
T13059	3524-3534	Gene_expression	denotes	expression
T13060	3524-3534	Gene_expression	denotes	expression
T13061	3386-3392	Regulation	denotes	effect
T13062	3592-3597	Protein	denotes	IκB-α
T13063	3612-3615	Protein	denotes	p65
T13064	3644-3659	Protein	denotes	luciferase gene
T13065	3736-3741	Protein	denotes	Tat R
T13066	3626-3636	Gene_expression	denotes	expression
T13067	3598-3607	Negative_regulation	denotes	inhibited
T13068	3616-3625	Positive_regulation	denotes	dependent
T13069	3803-3806	Protein	denotes	Tat
T13070	3823-3828	Protein	denotes	IκB-α
T13071	3847-3854	Protein	denotes	p65 DNA
T13072	3912-3917	Protein	denotes	IκB-α
T13073	3957-3960	Protein	denotes	p65
T13074	3807-3818	Binding	denotes	counteracts
T13075	3829-3839	Negative_regulation	denotes	repression
T13076	3855-3862	Binding	denotes	binding
T13077	3895-3906	Binding	denotes	associating
T13078	3946-3953	Binding	denotes	binding
T13079	3829-3839	Negative_regulation	denotes	repression
T26369	917-920	Protein	denotes	p65
T26370	930-935	Protein	denotes	IκB-α
T26371	936-946	Negative_regulation	denotes	inhibition
T26372	908-916	Negative_regulation	denotes	relieves
T26373	994-1013	Protein	denotes	mutant Tat proteins
T26374	1019-1021	Protein	denotes	HA
T26375	1022-1027	Protein	denotes	IκB-α
T26376	1075-1083	Protein	denotes	FLAG-Tat
T26377	1085-1089	Protein	denotes	FLAG
T26378	1090-1093	Protein	denotes	Tat
T26379	1229-1248	Protein	denotes	mutant Tat proteins
T26380	1444-1447	Protein	denotes	p65
T26381	1494-1496	Protein	denotes	HA
T26382	1497-1502	Protein	denotes	IκB-α
T26383	1536-1540	Protein	denotes	FLAG
T26384	1541-1544	Protein	denotes	Tat
T26385	1548-1552	Protein	denotes	FLAG
T26386	1553-1556	Protein	denotes	Tat
T26387	1585-1587	Protein	denotes	HA
T26388	1588-1593	Protein	denotes	IκB-α
T26389	1622-1638	Protein	denotes	anti-HA antibody
T26390	1898-1905	Protein	denotes	p65 DNA
T26391	1906-1913	Binding	denotes	binding
T26392	1995-1997	Protein	denotes	κB
T26393	2088-2090	Protein	denotes	HA
T26394	2091-2094	Protein	denotes	IκB
T26395	2105-2113	Protein	denotes	FLAG-Tat
T26396	2117-2121	Protein	denotes	FLAG
T26397	2122-2125	Protein	denotes	Tat
T26398	2243-2248	Protein	denotes	NF-κB
T26399	2497-2511	Protein	denotes	pCMV4-HA-IκB-α
T26400	2522-2533	Protein	denotes	p3xFLAG-Tat
T26401	2537-2544	Protein	denotes	p3xFLAG
T26402	2545-2548	Protein	denotes	Tat
T26403	2582-2592	Protein	denotes	luciferase
T26404	2673-2688	Protein	denotes	β-galactosidase
T26405	2762-2782	Protein	denotes	pκBLuc plasmid alone
T26406	2742-2754	Gene_expression	denotes	transfection
T26407	2933-2936	Protein	denotes	p65
T26408	2938-2945	Protein	denotes	anti-HA
T26409	2947-2956	Protein	denotes	anti-FLAG
T26410	2961-2986	Protein	denotes	anti-γ-tubulin antibodies
R10555	T13000	T13003	themeOf	Tat,counteracts
R10556	T13001	T13004	themeOf	IκB-α,inhibition
R10557	T13002	T13005	themeOf	p65,inhibition
R10558	T13006	T13008	themeOf	Tat,interaction
R10559	T13007	T13008	themeOf	IκB-α,interaction
R10560	T13013	T13015	themeOf	Tat,binding
R10561	T13014	T13015	themeOf	IκB-α,binding
R10562	T13016	T13024	themeOf	Tat,associating
R10563	T13016	T13030	causeOf	Tat,competed
R10564	T13017	T13024	themeOf	IκB-α,associating
R10565	T13018	T13025	themeOf	IκB-α,binding
R10566	T13019	T13025	themeOf	p65,binding
R10567	T13020	T13028	themeOf	Tat R,associate
R10568	T13021	T13026	themeOf	IκB-α,binding
R10569	T13022	T13027	themeOf	IκB-α,associate
R10570	T13022	T13028	themeOf	IκB-α,associate
R10571	T13023	T13029	themeOf	IκB-α,binding
R10572	T13025	T13030	themeOf	binding,competed
R10573	T13026	T13031	themeOf	binding,lacked
R10574	T13029	T13032	themeOf	binding,compete
R10575	T13033	T13040	themeOf	Tat,counteracted
R10576	T13035	T13041	themeOf	p65,binding
R10577	T13041	T13042	themeOf	binding,inhibition
R10578	T13043	T13046	themeOf	p65 DNA,binding
R10579	T13043	T13048	themeOf	p65 DNA,restored
R10580	T13044	T13047	causeOf	IκB-α,inhibited
R10581	T13045	T13048	causeOf	Tat R,restored
R10582	T13045	T13049	causeOf	Tat R,restored
R10583	T13046	T13047	themeOf	binding,inhibited
R10584	T13046	T13049	themeOf	binding,restored
R10585	T13050	T13061	causeOf	Tat,effect
R10586	T13051	T13058	themeOf	IκB-α,repression
R10587	T13055	T13059	themeOf	p65,expression
R10588	T13056	T13060	themeOf	IκB-α,expression
R10589	T13058	T13061	themeOf	repression,effect
R10590	T13062	T13067	causeOf	IκB-α,inhibited
R10591	T13063	T13068	causeOf	p65,dependent
R10592	T13064	T13066	themeOf	luciferase gene,expression
R10593	T13066	T13067	themeOf	expression,inhibited
R10594	T13066	T13068	themeOf	expression,dependent
R10595	T13069	T13074	themeOf	Tat,counteracts
R10596	T13070	T13075	themeOf	IκB-α,repression
R10597	T13070	T13076	themeOf	IκB-α,binding
R10598	T13071	T13076	themeOf	p65 DNA,binding
R10599	T13072	T13077	themeOf	IκB-α,associating
R10600	T13073	T13078	themeOf	p65,binding
R10601	T13076	T13079	themeOf	binding,repression
R20904	T26369	T26372	themeOf	p65,relieves
R20905	T26370	T26371	themeOf	IκB-α,inhibition
R20906	T26371	T26372	causeOf	inhibition,relieves
R20907	T26390	T26391	themeOf	p65 DNA,binding
R20908	T26405	T26406	themeOf	pκBLuc plasmid alone,transfection

test2

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T11416	0-3	Protein	denotes	Tat
T11417	4-15	Negative_regulation	denotes	counteracts
T11418	20-25	Protein	denotes	IκB-α
T11419	26-36	Negative_regulation	denotes	inhibition
T11420	40-43	Protein	denotes	p65
T11421	71-78	Binding	denotes	binding	true
T11422	116-127	Binding	denotes	interaction	true
T11423	131-134	Protein	denotes	Tat
T11424	140-145	Protein	denotes	IκB-α
T11425	263-272	Positive_regulation	denotes	following
T11426	273-276	Protein	denotes	Tat
T11427	286-301	Protein	denotes	Tat T,N(23,24)A
T11428	303-319	Protein	denotes	Tat C(22,25,27)A
T11429	321-334	Protein	denotes	Tat K(50,51)A
T11430	339-352	Protein	denotes	Tat R(49–57)A	true
T11431	366-371	Protein	denotes	IκB-α	true
T11432	372-392	Binding	denotes	coimmunoprecipitated
T11433	402-405	Protein	denotes	Tat		true
T11434	423-436	Protein	denotes	Tat R(49–57)A
T11435	483-503	Entity	denotes	arginine-rich domain
T11436	507-510	Protein	denotes	Tat
T11437	531-538	Binding	denotes	binding
T11438	542-547	Protein	denotes	IκB-α	true
T11439	616-619	Protein	denotes	Tat	true
T11440	620-631	Binding	denotes	associating
T11441	637-642	Protein	denotes	IκB-α
T11442	643-651	Negative_regulation	denotes	competed
T11443	656-663	Binding	denotes	binding	true
T11444	667-672	Protein	denotes	IκB-α	true
T11445	676-679	Protein	denotes	p65
T11446	737-750	Protein	denotes	Tat R(49–57)A
T11447	758-764	Negative_regulation	denotes	lacked
T11448	769-776	Binding	denotes	binding
T11449	786-791	Protein	denotes	IκB-α
T11450	801-810	Binding	denotes	associate
T11451	816-821	Protein	denotes	IκB-α
T11452	834-841	Negative_regulation	denotes	compete
T11453	846-853	Binding	denotes	binding
T11454	857-862	Protein	denotes	IκB-α
T11455	866-869	Protein	denotes	p65
T11456	3015-3018	Protein	denotes	Tat
T11457	3019-3031	Negative_regulation	denotes	counteracted
T11458	3036-3041	Protein	denotes	IκB-α
T11459	3042-3050	Positive_regulation	denotes	mediated
T11460	3051-3061	Negative_regulation	denotes	inhibition
T11461	3065-3068	Protein	denotes	p65
T11462	3069-3076	Binding	denotes	binding
T11463	3118-3121	Protein	denotes	p65
T11464	3126-3131	Protein	denotes	IκB-α
T11465	3177-3184	Negative_regulation	denotes	absence
T11466	3188-3191	Protein	denotes	Tat
T11467	3214-3217	Protein	denotes	p65
T11468	3222-3229	Binding	denotes	binding
T11469	3243-3252	Negative_regulation	denotes	inhibited
T11470	3256-3261	Protein	denotes	IκB-α
T11471	3285-3294	Regulation	denotes	dependent
T11472	3315-3318	Protein	denotes	Tat
T11473	3331-3343	Protein	denotes	Tat R(49–57)
T11474	3382-3385	Protein	denotes	Tat
T11475	3386-3392	Regulation	denotes	effect
T11476	3400-3405	Protein	denotes	IκB-α
T11477	3406-3416	Negative_regulation	denotes	repression
T11478	3420-3423	Protein	denotes	p65
T11479	3424-3439	Transcription	denotes	transcriptional
T11480	3465-3468	Protein	denotes	p50
T11481	3468-3469	Gene_expression	denotes	−
T11482	3471-3474	Protein	denotes	p65
T11483	3474-3476	Gene_expression	denotes	−/
T11484	3497-3500	Protein	denotes	Luc
T11485	3524-3534	Gene_expression	denotes	expression
T11486	3546-3549	Protein	denotes	p65
T11487	3554-3559	Protein	denotes	IκB-α
T11488	3576-3583	Negative_regulation	denotes	absence
T11489	3587-3590	Protein	denotes	Tat
T11490	3592-3597	Protein	denotes	IκB-α
T11491	3598-3607	Negative_regulation	denotes	inhibited
T11492	3612-3615	Protein	denotes	p65
T11493	3616-3625	Positive_regulation	denotes	dependent
T11494	3626-3636	Gene_expression	denotes	expression
T11495	3644-3654	Protein	denotes	luciferase
T11496	3690-3699	Regulation	denotes	dependent
T11497	3720-3723	Protein	denotes	Tat
T11498	3736-3749	Protein	denotes	Tat R(49–57)A
T11499	3803-3806	Protein	denotes	Tat
T11500	3807-3818	Negative_regulation	denotes	counteracts
T11501	3823-3828	Protein	denotes	IκB-α
T11502	3829-3839	Negative_regulation	denotes	repression
T11503	3847-3850	Protein	denotes	p65
T11504	3855-3862	Binding	denotes	binding
T11505	3867-3882	Transcription	denotes	transcriptional
T11506	3895-3906	Binding	denotes	associating
T11507	3912-3917	Protein	denotes	IκB-α
T11508	3922-3931	Negative_regulation	denotes	competing
T11509	3946-3953	Binding	denotes	binding
T11510	3957-3960	Protein	denotes	p65
T25130	904-907	Protein	denotes	Tat
T25131	908-916	Negative_regulation	denotes	relieves
T25132	917-920	Protein	denotes	p65		true
T25133	930-935	Protein	denotes	IκB-α		true
T25134	936-946	Negative_regulation	denotes	inhibition
T25135	1001-1004	Protein	denotes	Tat
T25136	1019-1027	Protein	denotes	HA-IκB-α
T25137	1064-1071	Negative_regulation	denotes	absence
T25138	1075-1083	Protein	denotes	FLAG-Tat
T25139	1085-1105	Protein	denotes	FLAG-Tat T,N(23,24)A
T25140	1107-1125	Protein	denotes	FLAG-Tat K(50,51)A
T25141	1127-1145	Protein	denotes	FLAG-Tat R(49–57)A
T25142	1149-1170	Protein	denotes	FLAG-Tat C(22,25,27)A
T25143	1236-1239	Protein	denotes	Tat
T25144	1444-1447	Protein	denotes	p65
T25145	1494-1502	Protein	denotes	HA-IκB-α
T25146	1525-1532	Negative_regulation	denotes	absence
T25147	1536-1544	Protein	denotes	FLAG-Tat
T25148	1548-1566	Protein	denotes	FLAG-Tat R(49–57)A
T25149	1585-1593	Protein	denotes	HA-IκB-α
T25150	1787-1790	Protein	denotes	p65
T25151	1898-1901	Protein	denotes	p65
T25152	1906-1913	Binding	denotes	binding
T25153	2049-2052	Protein	denotes	p65
T25154	2077-2084	Negative_regulation	denotes	absence
T25155	2088-2096	Protein	denotes	HA-IκB-α
T25156	2105-2113	Protein	denotes	FLAG-Tat
T25157	2117-2135	Protein	denotes	FLAG-Tat R(49–57)A
T25158	2170-2177	Binding	denotes	binding
T25159	2365-2368	Protein	denotes	p50
T25160	2371-2374	Protein	denotes	p65
T25161	2444-2449	Protein	denotes	β-Gal
T25162	2483-2486	Protein	denotes	p65
T25163	2503-2511	Protein	denotes	HA-IκB-α
T25164	2530-2533	Protein	denotes	Tat
T25165	2545-2548	Protein	denotes	Tat
T25166	2582-2592	Protein	denotes	luciferase
T25167	2673-2688	Protein	denotes	β-galactosidase
R9324	T11416	T11417	causeOf	Tat,counteracts
R9325	T11416	T11421	themeOf	Tat,binding
R9326	T11418	T11419	causeOf	IκB-α,inhibition
R9327	T11419	T11417	themeOf	inhibition,counteracts
R9328	T11420	T11419	themeOf	p65,inhibition
R9329	T11423	T11422	themeOf	Tat,interaction
R9330	T11424	T11422	themeOf	IκB-α,interaction
R9331	T11431	T11432	themeOf	IκB-α,coimmunoprecipitated
R9332	T11433	T11432	themeOf	Tat,coimmunoprecipitated
R9333	T11434	T11432	themeOf	Tat R(49–57)A,coimmunoprecipitated
R9334	T11435	T11436	partOf	arginine-rich domain,Tat
R9335	T11436	T11437	themeOf	Tat,binding
R9336	T11438	T11437	themeOf	IκB-α,binding
R9337	T11439	T11440	themeOf	Tat,associating
R9338	T11440	T11442	causeOf	associating,competed
R9339	T11441	T11440	themeOf	IκB-α,associating
R9340	T11443	T11442	themeOf	binding,competed
R9341	T11444	T11443	themeOf	IκB-α,binding
R9342	T11445	T11443	themeOf	p65,binding
R9343	T11446	T11450	themeOf	Tat R(49–57)A,associate
R9344	T11448	T11447	themeOf	binding,lacked
R9345	T11449	T11448	themeOf	IκB-α,binding
R9346	T11451	T11450	themeOf	IκB-α,associate
R9347	T11453	T11452	themeOf	binding,compete
R9348	T11454	T11453	themeOf	IκB-α,binding
R9349	T11455	T11453	themeOf	p65,binding
R9350	T11456	T11457	causeOf	Tat,counteracted
R9351	T11458	T11460	causeOf	IκB-α,inhibition
R9352	T11458	T11459	causeOf	IκB-α,mediated
R9353	T11459	T11457	themeOf	mediated,counteracted
R9354	T11460	T11459	themeOf	inhibition,mediated
R9355	T11461	T11462	themeOf	p65,binding
R9356	T11462	T11460	themeOf	binding,inhibition
R9357	T11466	T11465	themeOf	Tat,absence
R9358	T11467	T11468	themeOf	p65,binding
R9359	T11468	T11469	themeOf	binding,inhibited
R9360	T11470	T11469	causeOf	IκB-α,inhibited
R9361	T11472	T11469	causeOf	Tat,inhibited
R9362	T11473	T11469	causeOf	Tat R(49–57),inhibited
R9363	T11476	T11477	causeOf	IκB-α,repression
R9364	T11477	T11475	themeOf	repression,effect
R9365	T11478	T11479	themeOf	p65,transcriptional
R9366	T11479	T11477	themeOf	transcriptional,repression
R9367	T11486	T11485	themeOf	p65,expression
R9368	T11487	T11485	themeOf	IκB-α,expression
R9369	T11489	T11488	themeOf	Tat,absence
R9370	T11490	T11491	causeOf	IκB-α,inhibited
R9371	T11491	T11496	themeOf	inhibited,dependent
R9372	T11492	T11493	causeOf	p65,dependent
R9373	T11493	T11491	themeOf	dependent,inhibited
R9374	T11493	T11496	themeOf	dependent,dependent
R9375	T11494	T11493	themeOf	expression,dependent
R9376	T11494	T11496	themeOf	expression,dependent
R9377	T11495	T11494	themeOf	luciferase,expression
R9378	T11499	T11506	themeOf	Tat,associating
R9379	T11499	T11508	causeOf	Tat,competing
R9380	T11501	T11502	causeOf	IκB-α,repression
R9381	T11502	T11500	themeOf	repression,counteracts
R9382	T11503	T11504	themeOf	p65,binding
R9383	T11503	T11505	themeOf	p65,transcriptional
R9384	T11504	T11502	themeOf	binding,repression
R9385	T11505	T11502	themeOf	transcriptional,repression
R9386	T11506	T11500	causeOf	associating,counteracts
R9387	T11507	T11506	themeOf	IκB-α,associating
R9388	T11508	T11502	causeOf	competing,repression
R9389	T11508	T11500	causeOf	competing,counteracts
R9390	T11509	T11508	themeOf	binding,competing
R9391	T11510	T11509	themeOf	p65,binding
R20015	T25130	T25131	causeOf	Tat,relieves
R20016	T25132	T25134	themeOf	p65,inhibition
R20017	T25133	T25134	causeOf	IκB-α,inhibition
R20018	T25134	T25131	themeOf	inhibition,relieves
R20019	T25147	T25146	themeOf	FLAG-Tat,absence
R20020	T25151	T25152	themeOf	p65,binding
R20021	T25155	T25158	themeOf	HA-IκB-α,binding
R20022	T25155	T25154	themeOf	HA-IκB-α,absence
R20023	T25156	T25158	themeOf	FLAG-Tat,binding
R20024	T25157	T25158	themeOf	FLAG-Tat R(49–57)A,binding

bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T11511	0-3	Protein	denotes	Tat
T11512	4-15	Negative_regulation	denotes	counteracts
T11513	20-25	Protein	denotes	IκB-α
T11514	26-36	Negative_regulation	denotes	inhibition
T11515	40-43	Protein	denotes	p65
T11516	47-56	Negative_regulation	denotes	competing
T11517	71-78	Binding	denotes	binding
T11518	116-127	Binding	denotes	interaction
T11519	131-134	Protein	denotes	Tat
T11520	140-145	Protein	denotes	IκB-α
T11521	273-276	Protein	denotes	Tat
T11522	286-301	Protein	denotes	Tat T,N(23,24)A
T11523	303-319	Protein	denotes	Tat C(22,25,27)A
T11524	321-334	Protein	denotes	Tat K(50,51)A
T11525	339-352	Protein	denotes	Tat R(49–57)A
T11533	515-523	Positive_regulation	denotes	involved
T11534	531-538	Binding	denotes	binding
T11535	542-547	Protein	denotes	IκB-α
T11536	616-619	Protein	denotes	Tat
T11537	620-631	Binding	denotes	associating
T11538	637-642	Protein	denotes	IκB-α
T11539	643-651	Negative_regulation	denotes	competed
T11540	656-663	Binding	denotes	binding
T11541	667-672	Protein	denotes	IκB-α
T11542	676-679	Protein	denotes	p65
T11543	737-750	Protein	denotes	Tat R(49–57)A
T11544	758-764	Negative_regulation	denotes	lacked
T11545	769-776	Binding	denotes	binding
T11546	777-781	Entity	denotes	site
T11547	786-791	Protein	denotes	IκB-α
T11548	801-810	Binding	denotes	associate	true
T11549	816-821	Protein	denotes	IκB-α
T11550	834-841	Negative_regulation	denotes	compete	true
T11551	846-853	Binding	denotes	binding
T11552	857-862	Protein	denotes	IκB-α
T11553	866-869	Protein	denotes	p65
T11554	3015-3018	Protein	denotes	Tat
T11555	3019-3031	Negative_regulation	denotes	counteracted		true
T11556	3036-3041	Protein	denotes	IκB-α
T11557	3051-3061	Negative_regulation	denotes	inhibition
T11558	3065-3068	Protein	denotes	p65
T11559	3069-3076	Binding	denotes	binding
T11560	3118-3121	Protein	denotes	p65
T11561	3126-3131	Protein	denotes	IκB-α
T11562	3188-3191	Protein	denotes	Tat
T11563	3214-3217	Protein	denotes	p65
T11564	3222-3229	Binding	denotes	binding
T11565	3243-3252	Negative_regulation	denotes	inhibited
T11566	3256-3261	Protein	denotes	IκB-α
T11567	3266-3274	Negative_regulation	denotes	restored	true
T11568	3266-3274	Negative_regulation	denotes	restored	true
T11569	3315-3318	Protein	denotes	Tat
T11570	3331-3343	Protein	denotes	Tat R(49–57)
T11571	3382-3385	Protein	denotes	Tat
T11572	3400-3405	Protein	denotes	IκB-α
T11573	3420-3423	Protein	denotes	p65
T11574	3465-3468	Protein	denotes	p50
T11575	3471-3474	Protein	denotes	p65
T11576	3497-3500	Protein	denotes	Luc
T11577	3546-3549	Protein	denotes	p65
T11578	3554-3559	Protein	denotes	IκB-α
T11579	3587-3590	Protein	denotes	Tat
T11580	3592-3597	Protein	denotes	IκB-α
T11581	3598-3607	Negative_regulation	denotes	inhibited
T11582	3612-3615	Protein	denotes	p65
T11583	3616-3625	Positive_regulation	denotes	dependent
T11584	3626-3636	Gene_expression	denotes	expression
T11585	3644-3654	Protein	denotes	luciferase
T11586	3671-3679	Negative_regulation	denotes	restored	true
T11587	3671-3679	Negative_regulation	denotes	restored	true
T11588	3720-3723	Protein	denotes	Tat
T11589	3736-3749	Protein	denotes	Tat R(49–57)A
T11590	3803-3806	Protein	denotes	Tat
T11591	3807-3818	Negative_regulation	denotes	counteracts
T11592	3807-3818	Negative_regulation	denotes	counteracts
T11593	3823-3828	Protein	denotes	IκB-α
T11594	3829-3839	Negative_regulation	denotes	repression
T11595	3847-3850	Protein	denotes	p65
T11596	3855-3862	Binding	denotes	binding
T11597	3895-3906	Binding	denotes	associating
T11598	3912-3917	Protein	denotes	IκB-α
T11599	3922-3931	Negative_regulation	denotes	competing
T11600	3946-3953	Binding	denotes	binding
T11601	3957-3960	Protein	denotes	p65
T25194	2170-2177	Binding	denotes	binding
T25195	2365-2368	Protein	denotes	p50
T25196	2371-2374	Protein	denotes	p65
T25197	2444-2449	Protein	denotes	β-Gal
T25198	2483-2486	Protein	denotes	p65
T25199	2503-2511	Protein	denotes	HA-IκB-α
T25200	2530-2533	Protein	denotes	Tat
T25201	2545-2548	Protein	denotes	Tat
T25202	2582-2592	Protein	denotes	luciferase
T25203	2673-2688	Protein	denotes	β-galactosidase
T11526	366-371	Protein	denotes	IκB-α
T11527	372-392	Binding	denotes	coimmunoprecipitated	true
T11528	372-392	Binding	denotes	coimmunoprecipitated	true
T11529	402-405	Protein	denotes	Tat
T11530	423-436	Protein	denotes	Tat R(49–57)A
T11531	483-503	Entity	denotes	arginine-rich domain
T11532	507-510	Protein	denotes	Tat
T25168	904-907	Protein	denotes	Tat
T25169	908-916	Negative_regulation	denotes	relieves
T25170	917-920	Protein	denotes	p65
T25171	930-935	Protein	denotes	IκB-α
T25172	936-946	Negative_regulation	denotes	inhibition
T25173	1001-1004	Protein	denotes	Tat
T25174	1019-1027	Protein	denotes	HA-IκB-α
T25175	1075-1083	Protein	denotes	FLAG-Tat
T25176	1085-1105	Protein	denotes	FLAG-Tat T,N(23,24)A
T25177	1107-1125	Protein	denotes	FLAG-Tat K(50,51)A
T25178	1127-1145	Protein	denotes	FLAG-Tat R(49–57)A
T25179	1149-1170	Protein	denotes	FLAG-Tat C(22,25,27)A
T25180	1236-1239	Protein	denotes	Tat
T25181	1444-1447	Protein	denotes	p65
T25182	1494-1502	Protein	denotes	HA-IκB-α
T25183	1536-1544	Protein	denotes	FLAG-Tat
T25184	1548-1566	Protein	denotes	FLAG-Tat R(49–57)A
T25185	1585-1593	Protein	denotes	HA-IκB-α
T25186	1787-1790	Protein	denotes	p65
T25187	1898-1901	Protein	denotes	p65
T25188	1906-1913	Binding	denotes	binding		true
T25189	2049-2052	Protein	denotes	p65
T25190	2088-2096	Protein	denotes	HA-IκB-α
T25191	2105-2113	Protein	denotes	FLAG-Tat
T25192	2117-2135	Protein	denotes	FLAG-Tat R(49–57)A
T25193	2151-2162	Negative_regulation	denotes	competition		true
R9393	T11513	T11517	themeOf	IκB-α,binding
R9399	T11519	T11518	themeOf	Tat,interaction
R9400	T11520	T11518	themeOf	IκB-α,interaction
R9401	T11526	T11528	themeOf	IκB-α,coimmunoprecipitated
R9402	T11526	T11527	themeOf	IκB-α,coimmunoprecipitated
R9403	T11529	T11528	themeOf	Tat,coimmunoprecipitated
R9404	T11530	T11527	themeOf	Tat R(49–57)A,coimmunoprecipitated
R9405	T11531	T11533	causeOf	arginine-rich domain,involved
R9406	T11531	T11532	partOf	arginine-rich domain,Tat
R9407	T11532	T11534	themeOf	Tat,binding
R9408	T11534	T11533	themeOf	binding,involved
R9409	T11535	T11534	themeOf	IκB-α,binding
R9410	T11536	T11537	themeOf	Tat,associating
R9411	T11537	T11539	causeOf	associating,competed
R9412	T11538	T11537	themeOf	IκB-α,associating
R9413	T11540	T11539	themeOf	binding,competed
R9414	T11541	T11540	themeOf	IκB-α,binding
R9415	T11542	T11540	themeOf	p65,binding
R9418	T11546	T11544	themeOf	site,lacked
R9419	T11546	T11536	partOf	site,Tat
R9445	T11588	T11586	causeOf	Tat,restored
R9446	T11589	T11587	causeOf	Tat R(49–57)A,restored
R9447	T11590	T11597	themeOf	Tat,associating
R9448	T11590	T11599	causeOf	Tat,competing
R9449	T11593	T11594	causeOf	IκB-α,repression
R9450	T11594	T11591	themeOf	repression,counteracts
R9451	T11594	T11592	themeOf	repression,counteracts
R9452	T11595	T11596	themeOf	p65,binding
R9453	T11596	T11594	themeOf	binding,repression
R9454	T11597	T11591	causeOf	associating,counteracts
R9455	T11598	T11597	themeOf	IκB-α,associating
R9456	T11598	T11600	themeOf	IκB-α,binding
R9457	T11599	T11592	causeOf	competing,counteracts
R9458	T11600	T11599	themeOf	binding,competing
R9459	T11601	T11600	themeOf	p65,binding
R9392	T11511	T11516	causeOf	Tat,competing
R9394	T11513	T11514	causeOf	IκB-α,inhibition
R9395	T11514	T11512	themeOf	inhibition,counteracts
R9396	T11515	T11514	themeOf	p65,inhibition
R9397	T11516	T11512	causeOf	competing,counteracts
R9398	T11517	T11516	themeOf	binding,competing
R9416	T11543	T11548	themeOf	Tat R(49–57)A,associate
R9417	T11543	T11550	causeOf	Tat R(49–57)A,compete
R9420	T11546	T11545	themeOf	site,binding
R9421	T11547	T11545	themeOf	IκB-α,binding
R9422	T11549	T11548	themeOf	IκB-α,associate
R9423	T11551	T11550	themeOf	binding,compete
R9424	T11552	T11551	themeOf	IκB-α,binding
R9425	T11553	T11551	themeOf	p65,binding
R9426	T11554	T11555	causeOf	Tat,counteracted
R9427	T11556	T11557	causeOf	IκB-α,inhibition
R9428	T11557	T11555	themeOf	inhibition,counteracted
R9429	T11558	T11559	themeOf	p65,binding
R9430	T11559	T11557	themeOf	binding,inhibition
R9431	T11563	T11564	themeOf	p65,binding
R9432	T11564	T11565	themeOf	binding,inhibited
R9433	T11565	T11568	themeOf	inhibited,restored
R9434	T11565	T11567	themeOf	inhibited,restored
R9435	T11566	T11565	causeOf	IκB-α,inhibited
R9436	T11569	T11568	causeOf	Tat,restored
R9437	T11570	T11567	causeOf	Tat R(49–57),restored
R9438	T11580	T11581	causeOf	IκB-α,inhibited
R9439	T11581	T11586	themeOf	inhibited,restored
R9440	T11581	T11587	themeOf	inhibited,restored
R9441	T11582	T11583	causeOf	p65,dependent
R9442	T11583	T11581	themeOf	dependent,inhibited
R9443	T11584	T11583	themeOf	expression,dependent
R9444	T11585	T11584	themeOf	luciferase,expression
R20025	T25168	T25169	causeOf	Tat,relieves
R20026	T25170	T25172	themeOf	p65,inhibition
R20027	T25171	T25172	causeOf	IκB-α,inhibition
R20028	T25172	T25169	themeOf	inhibition,relieves
R20029	T25187	T25188	themeOf	p65,binding
R20030	T25189	T25194	themeOf	p65,binding
R20031	T25190	T25193	causeOf	HA-IκB-α,competition
R20032	T25194	T25193	themeOf	binding,competition

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T11967	0-3	P04608	denotes	Tat
T11968	0-3	P04610	denotes	Tat
T11969	0-3	P04605	denotes	Tat
T11970	0-3	Q8UMQ1	denotes	Tat
T11971	40-43	P21579	denotes	p65
T11972	40-43	Q04206	denotes	p65
T11973	131-134	P04610	denotes	Tat
T11974	131-134	Q8UMQ1	denotes	Tat
T11975	131-134	P04605	denotes	Tat
T11976	131-134	P04608	denotes	Tat
T11977	273-276	Q8UMQ1	denotes	Tat
T11978	273-276	P04610	denotes	Tat
T11979	273-276	P04608	denotes	Tat
T11980	273-276	P04605	denotes	Tat
T11981	286-289	P04608	denotes	Tat
T11982	286-289	P04610	denotes	Tat
T11983	286-289	Q8UMQ1	denotes	Tat
T11984	286-289	P04605	denotes	Tat
T11985	303-306	P04610	denotes	Tat
T11986	303-306	P04608	denotes	Tat
T11987	303-306	P04605	denotes	Tat
T11988	303-306	Q8UMQ1	denotes	Tat
T11989	321-324	Q8UMQ1	denotes	Tat
T11990	321-324	P04610	denotes	Tat
T11991	321-324	P04608	denotes	Tat
T11992	321-324	P04605	denotes	Tat
T11993	339-342	P04608	denotes	Tat
T11994	339-342	P04610	denotes	Tat
T11995	339-342	Q8UMQ1	denotes	Tat
T11996	339-342	P04605	denotes	Tat
T11997	402-405	Q8UMQ1	denotes	Tat
T11998	402-405	P04610	denotes	Tat
T11999	402-405	P04608	denotes	Tat
T12000	402-405	P04605	denotes	Tat
T12001	423-426	P04610	denotes	Tat
T12002	423-426	Q8UMQ1	denotes	Tat
T12003	423-426	P04608	denotes	Tat
T12004	423-426	P04605	denotes	Tat
T12005	507-510	Q8UMQ1	denotes	Tat
T12006	507-510	P04610	denotes	Tat
T12007	507-510	P04608	denotes	Tat
T12008	507-510	P04605	denotes	Tat
T12009	616-619	Q8UMQ1	denotes	Tat
T12010	616-619	P04610	denotes	Tat
T12011	616-619	P04608	denotes	Tat
T12012	616-619	P04605	denotes	Tat
T12013	676-679	Q04206	denotes	p65
T12014	676-679	P21579	denotes	p65
T12015	737-740	P04610	denotes	Tat
T12016	737-740	Q8UMQ1	denotes	Tat
T12017	737-740	P04608	denotes	Tat
T12018	737-740	P04605	denotes	Tat
T12019	866-869	Q04206	denotes	p65
T12020	866-869	P21579	denotes	p65
T12021	3015-3018	P04610	denotes	Tat
T12022	3015-3018	P04608	denotes	Tat
T12023	3015-3018	Q8UMQ1	denotes	Tat
T12024	3015-3018	P04605	denotes	Tat
T12025	3065-3068	Q04206	denotes	p65
T12026	3065-3068	P21579	denotes	p65
T12027	3118-3121	Q04206	denotes	p65
T12028	3118-3121	P21579	denotes	p65
T12029	3188-3191	P04605	denotes	Tat
T12030	3188-3191	Q8UMQ1	denotes	Tat
T12031	3188-3191	P04608	denotes	Tat
T12032	3188-3191	P04610	denotes	Tat
T12033	3214-3217	P21579	denotes	p65
T12034	3214-3217	Q04206	denotes	p65
T12035	3315-3318	P04608	denotes	Tat
T12036	3315-3318	Q8UMQ1	denotes	Tat
T12037	3315-3318	P04610	denotes	Tat
T12038	3315-3318	P04605	denotes	Tat
T12039	3331-3334	P04608	denotes	Tat
T12040	3331-3334	Q8UMQ1	denotes	Tat
T12041	3331-3334	P04610	denotes	Tat
T12042	3331-3334	P04605	denotes	Tat
T12043	3382-3385	P04608	denotes	Tat
T12044	3382-3385	Q8UMQ1	denotes	Tat
T12045	3382-3385	P04610	denotes	Tat
T12046	3382-3385	P04605	denotes	Tat
T12047	3420-3423	Q04206	denotes	p65
T12048	3420-3423	P21579	denotes	p65
T12049	3465-3468	P19838	denotes	p50
T12050	3471-3474	P21579	denotes	p65
T12051	3471-3474	Q04206	denotes	p65
T12052	3546-3549	P21579	denotes	p65
T12053	3546-3549	Q04206	denotes	p65
T12054	3587-3590	Q8UMQ1	denotes	Tat
T12055	3587-3590	P04610	denotes	Tat
T12056	3587-3590	P04605	denotes	Tat
T12057	3587-3590	P04608	denotes	Tat
T12058	3612-3615	Q04206	denotes	p65
T12059	3612-3615	P21579	denotes	p65
T12060	3644-3654	P08659	denotes	luciferase
T12061	3720-3723	P04610	denotes	Tat
T12062	3720-3723	P04608	denotes	Tat
T12063	3720-3723	P04605	denotes	Tat
T12064	3720-3723	Q8UMQ1	denotes	Tat
T12065	3736-3739	Q8UMQ1	denotes	Tat
T12066	3736-3739	P04610	denotes	Tat
T12067	3736-3739	P04605	denotes	Tat
T12068	3736-3739	P04608	denotes	Tat
T12069	3803-3806	P04608	denotes	Tat
T12070	3803-3806	P04610	denotes	Tat
T12071	3803-3806	Q8UMQ1	denotes	Tat
T12072	3803-3806	P04605	denotes	Tat
T12073	3847-3850	Q04206	denotes	p65
T12074	3847-3850	P21579	denotes	p65
T12075	3957-3960	Q04206	denotes	p65
T12076	3957-3960	P21579	denotes	p65
T25592	904-907	P04608	denotes	Tat
T25593	904-907	Q8UMQ1	denotes	Tat
T25594	904-907	P04610	denotes	Tat
T25595	904-907	P04605	denotes	Tat
T25596	917-920	Q04206	denotes	p65
T25597	917-920	P21579	denotes	p65
T25598	1001-1004	P04605	denotes	Tat
T25599	1001-1004	Q8UMQ1	denotes	Tat
T25600	1001-1004	P04610	denotes	Tat
T25601	1001-1004	P04608	denotes	Tat
T25602	1080-1083	P04605	denotes	Tat
T25603	1080-1083	P04608	denotes	Tat
T25604	1080-1083	Q8UMQ1	denotes	Tat
T25605	1080-1083	P04610	denotes	Tat
T25606	1090-1093	Q8UMQ1	denotes	Tat
T25607	1090-1093	P04610	denotes	Tat
T25608	1090-1093	P04608	denotes	Tat
T25609	1090-1093	P04605	denotes	Tat
T25610	1112-1115	Q8UMQ1	denotes	Tat
T25611	1112-1115	P04610	denotes	Tat
T25612	1112-1115	P04608	denotes	Tat
T25613	1112-1115	P04605	denotes	Tat
T25614	1132-1135	P04610	denotes	Tat
T25615	1132-1135	P04608	denotes	Tat
T25616	1132-1135	P04605	denotes	Tat
T25617	1132-1135	Q8UMQ1	denotes	Tat
T25618	1154-1157	P04610	denotes	Tat
T25619	1154-1157	Q8UMQ1	denotes	Tat
T25620	1154-1157	P04608	denotes	Tat
T25621	1154-1157	P04605	denotes	Tat
T25622	1236-1239	Q8UMQ1	denotes	Tat
T25623	1236-1239	P04608	denotes	Tat
T25624	1236-1239	P04610	denotes	Tat
T25625	1236-1239	P04605	denotes	Tat
T25626	1444-1447	P21579	denotes	p65
T25627	1444-1447	Q04206	denotes	p65
T25628	1541-1544	Q8UMQ1	denotes	Tat
T25629	1541-1544	P04605	denotes	Tat
T25630	1541-1544	P04608	denotes	Tat
T25631	1541-1544	P04610	denotes	Tat
T25632	1553-1556	P04610	denotes	Tat
T25633	1553-1556	P04608	denotes	Tat
T25634	1553-1556	P04605	denotes	Tat
T25635	1553-1556	Q8UMQ1	denotes	Tat
T25636	1787-1790	P21579	denotes	p65
T25637	1787-1790	Q04206	denotes	p65
T25638	1898-1901	Q04206	denotes	p65
T25639	1898-1901	P21579	denotes	p65
T25640	2049-2052	P21579	denotes	p65
T25641	2049-2052	Q04206	denotes	p65
T25642	2110-2113	P04610	denotes	Tat
T25643	2110-2113	Q8UMQ1	denotes	Tat
T25644	2110-2113	P04608	denotes	Tat
T25645	2110-2113	P04605	denotes	Tat
T25646	2122-2125	Q8UMQ1	denotes	Tat
T25647	2122-2125	P04608	denotes	Tat
T25648	2122-2125	P04610	denotes	Tat
T25649	2122-2125	P04605	denotes	Tat
T25650	2365-2368	P19838	denotes	p50
T25651	2371-2374	Q04206	denotes	p65
T25652	2371-2374	P21579	denotes	p65
T25653	2483-2486	Q04206	denotes	p65
T25654	2483-2486	P21579	denotes	p65
T25655	2530-2533	Q8UMQ1	denotes	Tat
T25656	2530-2533	P04608	denotes	Tat
T25657	2530-2533	P04610	denotes	Tat
T25658	2530-2533	P04605	denotes	Tat
T25659	2545-2548	Q8UMQ1	denotes	Tat
T25660	2545-2548	P04610	denotes	Tat
T25661	2545-2548	P04608	denotes	Tat
T25662	2545-2548	P04605	denotes	Tat
T25663	2582-2592	P08659	denotes	luciferase
T25664	2730-2738	Q04864	denotes	relative
T25665	2933-2936	P21579	denotes	p65
T25666	2933-2936	Q04206	denotes	p65

PMC:3333881 / 22496-26457 JSONTXT

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PMC:3333881 / 22496-26457 JSON TXT