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PMC:3333881 / 12365-14746 JSONTXT

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bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T7546 0-9 NNP denotes Chromatin
T7547 10-29 NN denotes immunoprecipitation
T7548 30-35 NN denotes assay
T7549 36-41 NNS denotes Cells
T7550 42-46 VBD denotes were
T7551 47-52 VBN denotes fixed
T7552 53-55 IN denotes by
T7553 56-62 VBG denotes adding
T7554 63-75 NN denotes formaldehyde
T7555 76-77 -LRB- denotes (
T7556 77-90 NNP denotes Sigma-Aldrich
T7589 245-248 NNP denotes PBS
T7590 248-249 , denotes ,
T7591 250-253 CC denotes and
T7592 254-261 VBD denotes scraped
T7593 261-262 . denotes .
T7594 263-268 IN denotes After
T7595 269-283 NN denotes centrifugation
T7596 283-284 , denotes ,
T7597 285-290 NNS denotes cells
T7598 291-295 VBD denotes were
T7599 296-298 CD denotes 10
T7600 299-302 NN denotes min
T7601 303-308 VBN denotes lysed
T7602 309-311 IN denotes in
T7603 312-317 NN denotes lysis
T7604 318-324 NN denotes buffer
T7605 325-326 -LRB- denotes (
T7606 326-327 CD denotes 5
T7607 328-330 NNP denotes mM
T7608 331-336 NNP denotes PIPES
T7609 337-339 NNP denotes pH
T7610 340-343 CD denotes 8.0
T7611 343-344 , denotes ,
T7612 345-347 CD denotes 85
T7613 348-350 NNP denotes mM
T7614 351-354 NNP denotes KCl
T7615 354-355 , denotes ,
T7616 356-359 CD denotes 0.5
T7617 359-360 NN denotes %
T7618 361-366 NN denotes NP-40
T7619 366-367 -RRB- denotes )
T7620 368-380 VBN denotes supplemented
T7621 381-385 IN denotes with
T7622 386-387 CD denotes 1
T7623 387-388 CD denotes ×
T7624 389-397 JJ denotes Complete
T7625 398-406 NNP denotes Protease
T7626 407-416 NNP denotes Inhibitor
T7627 417-418 -LRB- denotes (
T7628 418-423 NNP denotes Roche
T7629 424-434 NNP denotes Diagnostic
T7630 435-439 NNP denotes GmbH
T7631 439-440 -RRB- denotes )
T7632 440-441 . denotes .
T7633 442-448 NNP denotes Nuclei
T7634 449-453 VBD denotes were
T7635 454-463 VBN denotes pelletted
T7636 464-465 -LRB- denotes (
T7637 465-469 CD denotes 1000
T7638 470-471 NN denotes ×
T7639 472-473 NN denotes g
T7640 473-474 , denotes ,
T7641 475-476 CD denotes 5
T7642 477-480 NN denotes min
T7643 480-481 -RRB- denotes )
T7644 481-482 , denotes ,
T7645 483-486 CC denotes and
T7646 487-498 VBD denotes resuspended
T7647 499-501 IN denotes in
T7648 502-512 NN denotes sonication
T7649 513-519 NN denotes buffer
T7650 520-521 -LRB- denotes (
T7651 521-523 CD denotes 50
T7652 524-526 NNP denotes mM
T7653 527-531 NNP denotes Tris
T7654 532-535 NNP denotes HCl
T7655 536-538 NNP denotes pH
T7656 539-542 CD denotes 8.0
T7657 542-543 , denotes ,
T7658 544-545 CD denotes 1
T7659 545-546 NN denotes %
T7660 547-550 NNP denotes SDS
T7661 550-551 , denotes ,
T7662 552-554 CD denotes 10
T7663 555-557 NNP denotes mM
T7664 558-562 NNP denotes EDTA
T7665 562-563 -RRB- denotes )
T7666 563-564 . denotes .
T7667 565-574 NNP denotes Chromatin
T7668 575-578 VBD denotes was
T7669 579-588 VBN denotes sonicated
T7670 589-594 VBG denotes using
T7671 595-603 NNP denotes Bandelin
T7672 604-612 NNPS denotes Sonoplus
T7673 613-617 NNP denotes GM70
T7674 618-619 -LRB- denotes (
T7675 619-627 NNP denotes Bandelin
T7676 628-638 NNP denotes Electronic
T7677 638-639 , denotes ,
T7678 640-646 NNP denotes Berlin
T7679 646-647 , denotes ,
T7680 648-655 NNP denotes Germany
T7681 655-656 -RRB- denotes )
T7682 656-657 , denotes ,
T7683 658-669 VBN denotes centrifuged
T7684 670-671 -LRB- denotes (
T7685 671-673 CD denotes 14
T7686 674-677 CD denotes 000
T7687 678-679 NN denotes ×
T7688 680-681 NN denotes g
T7689 681-682 , denotes ,
T7690 683-685 CD denotes 15
T7691 686-689 NN denotes min
T7692 689-690 -RRB- denotes )
T7693 690-691 , denotes ,
T7694 692-695 CC denotes and
T7695 696-707 NN denotes supernatant
T7696 708-711 VBD denotes was
T7697 712-719 JJ denotes 10-fold
T7698 720-727 VBN denotes diluted
T7699 728-730 IN denotes in
T7700 731-739 NN denotes dilution
T7701 740-746 NN denotes buffer
T7702 747-748 -LRB- denotes (
T7703 748-752 CD denotes 0.01
T7704 752-753 NN denotes %
T7705 754-757 NNP denotes SDS
T7706 757-758 , denotes ,
T7707 759-763 CD denotes 16.7
T7708 764-766 NNP denotes mM
T7709 767-771 NNP denotes Tris
T7710 772-775 NNP denotes HCl
T7711 776-778 NNP denotes pH
T7712 779-782 CD denotes 8.0
T7713 782-783 , denotes ,
T7714 784-787 CD denotes 1.1
T7715 787-788 NN denotes %
T7716 789-795 NNP denotes Triton
T7717 796-801 NNP denotes X-100
T7718 801-802 , denotes ,
T7719 803-806 CD denotes 167
T7720 807-809 NNP denotes mM
T7721 810-814 NNP denotes NaCl
T7722 814-815 , denotes ,
T7723 816-819 CD denotes 1.2
T7724 820-822 NNP denotes mM
T7725 823-827 NNP denotes EDTA
T7726 827-828 -RRB- denotes )
T7727 828-829 . denotes .
T7728 830-837 NNS denotes Samples
T7729 838-842 VBD denotes were
T7730 843-854 JJ denotes pre-cleared
T7731 855-857 IN denotes by
T7732 858-861 JJ denotes 3-h
T7733 862-872 NN denotes incubation
T7734 873-877 IN denotes with
T7735 878-880 CD denotes 20
T7736 881-883 NN denotes µl
T7737 884-886 IN denotes of
T7738 887-894 NN denotes protein
T7739 895-896 NNP denotes G
T7740 897-904 JJ denotes agarose
T7741 905-910 NNS denotes beads
T7742 911-919 VBN denotes followed
T7743 920-922 IN denotes by
T7744 923-933 NN denotes incubation
T7745 934-938 IN denotes with
T7746 939-949 NNS denotes antibodies
T7747 950-957 IN denotes against
T7748 958-961 DT denotes the
T7749 962-970 JJ denotes analysed
T7750 971-979 NNS denotes proteins
T7751 979-980 . denotes .
T7752 981-988 JJ denotes Primary
T7753 989-999 NNS denotes antibodies
T7754 1000-1004 VBD denotes were
T7755 1004-1005 : denotes :
T7756 1006-1014 JJ denotes anti-p65
T7757 1015-1016 -LRB- denotes (
T7758 1016-1022 JJ denotes sc-372
T7759 1022-1023 -RRB- denotes )
T7760 1023-1024 , denotes ,
T7761 1025-1034 JJ denotes anti-IκBα
T7762 1035-1036 -LRB- denotes (
T7763 1036-1042 JJ denotes sc-203
T7764 1042-1043 -RRB- denotes )
T7765 1044-1047 CC denotes and
T7766 1048-1054 NN denotes rabbit
T7767 1055-1058 NNP denotes IgG
T7768 1059-1060 -LRB- denotes (
T7769 1060-1067 JJ denotes sc-2027
T7770 1067-1068 -RRB- denotes )
T7771 1069-1073 IN denotes from
T7772 1074-1079 NNP denotes Santa
T7773 1080-1084 NNP denotes Cruz
T7774 1085-1098 NNP denotes Biotechnology
T7775 1098-1099 : denotes ;
T7776 1100-1109 NNP denotes anti-FLAG
T7777 1110-1112 NNP denotes M2
T7778 1113-1117 IN denotes from
T7779 1118-1123 NNP denotes Sigma
T7780 1124-1131 NNP denotes Aldrich
T7781 1131-1132 . denotes .
T7782 1133-1153 NNS denotes Immunoprecipitations
T7783 1154-1158 VBD denotes were
T7784 1159-1166 VBN denotes carried
T7785 1167-1170 RP denotes out
T7786 1171-1173 IN denotes at
T7787 1174-1175 CD denotes 4
T7788 1175-1176 CD denotes °
T7789 1176-1177 NNP denotes C
T7790 1178-1187 JJ denotes overnight
T7791 1188-1191 CC denotes and
T7792 1192-1198 JJ denotes immune
T7793 1199-1208 NNS denotes complexes
T7794 1209-1213 VBD denotes were
T7795 1214-1223 VBN denotes collected
T7796 1224-1228 IN denotes with
T7797 1229-1236 NN denotes protein
T7798 1237-1238 NNP denotes G
T7799 1239-1246 JJ denotes agarose
T7800 1247-1252 NNS denotes beads
T7801 1252-1253 , denotes ,
T7802 1254-1260 VBD denotes washed
T7803 1261-1265 CD denotes five
T7804 1266-1271 NNS denotes times
T7805 1272-1276 IN denotes with
T7806 1277-1280 JJ denotes low
T7807 1281-1285 NN denotes salt
T7808 1286-1292 NN denotes buffer
T7809 1293-1294 -LRB- denotes (
T7810 1294-1296 CD denotes 20
T7811 1297-1299 NNP denotes mM
T7812 1300-1304 NNP denotes Tris
T7813 1305-1308 NNP denotes HCl
T7814 1309-1311 NNP denotes pH
T7815 1312-1315 CD denotes 8.0
T7816 1315-1316 , denotes ,
T7817 1317-1320 CD denotes 0.1
T7818 1320-1321 NN denotes %
T7819 1322-1325 NNP denotes SDS
T7820 1325-1326 , denotes ,
T7821 1327-1328 CD denotes 1
T7822 1328-1329 NN denotes %
T7823 1330-1336 NNP denotes Triton
T7824 1337-1342 NNP denotes X-100
T7825 1342-1343 , denotes ,
T7826 1344-1345 CD denotes 2
T7827 1346-1348 NNP denotes mM
T7828 1349-1353 NNP denotes EDTA
T7829 1353-1354 , denotes ,
T7830 1355-1358 CD denotes 150
T7831 1359-1361 NNP denotes mM
T7832 1362-1366 NNP denotes NaCl
T7833 1366-1367 -RRB- denotes )
T7834 1367-1368 , denotes ,
T7835 1369-1373 CD denotes four
T7836 1374-1379 NNS denotes times
T7837 1380-1384 IN denotes with
T7838 1385-1389 JJ denotes high
T7839 1390-1394 NN denotes salt
T7840 1395-1401 NN denotes buffer
T7841 1402-1403 -LRB- denotes (
T7842 1403-1405 CD denotes 20
T7843 1406-1408 NNP denotes mM
T7844 1409-1413 NNP denotes Tris
T7845 1414-1417 NNP denotes HCl
T7846 1418-1420 NNP denotes pH
T7847 1421-1424 CD denotes 8.0
T7848 1424-1425 , denotes ,
T7849 1426-1429 CD denotes 0.1
T7850 1429-1430 NN denotes %
T7851 1431-1434 NNP denotes SDS
T7852 1434-1435 , denotes ,
T7853 1436-1437 CD denotes 1
T7854 1437-1438 NN denotes %
T7855 1439-1445 NNP denotes Triton
T7856 1446-1451 NNP denotes X-100
T7857 1451-1452 , denotes ,
T7858 1453-1454 CD denotes 2
T7859 1455-1457 NNP denotes mM
T7860 1458-1462 NNP denotes EDTA
T7861 1462-1463 , denotes ,
T7862 1464-1467 CD denotes 500
T7863 1468-1470 NNP denotes mM
T7864 1471-1475 NNP denotes NaCl
T7865 1475-1476 -RRB- denotes )
T7866 1476-1477 , denotes ,
T7867 1478-1482 RB denotes once
T7868 1483-1487 IN denotes with
T7869 1488-1490 NNP denotes TE
T7870 1491-1497 NN denotes buffer
T7871 1498-1499 -LRB- denotes (
T7872 1499-1501 CD denotes 10
T7873 1502-1504 NNP denotes mM
T7874 1505-1509 NNP denotes Tris
T7875 1510-1513 NNP denotes HCl
T7876 1514-1516 NNP denotes pH
T7877 1517-1520 CD denotes 8.0
T7878 1520-1521 , denotes ,
T7879 1522-1523 CD denotes 1
T7915 1702-1705 NN denotes kit
T7916 1706-1707 -LRB- denotes (
T7917 1707-1713 NNP denotes QIAGEN
T7918 1713-1714 -RRB- denotes )
T7919 1715-1718 CC denotes and
T7920 1719-1725 VBD denotes eluted
T7921 1726-1728 IN denotes in
T7922 1729-1731 CD denotes 50
T7923 1732-1734 NN denotes µl
T7924 1735-1742 JJ denotes sterile
T7925 1743-1752 JJ denotes distilled
T7926 1753-1758 NN denotes water
T7927 1758-1759 . denotes .
T7928 1760-1768 JJ denotes Specific
T7929 1769-1779 NN denotes enrichment
T7930 1780-1782 IN denotes in
T7931 1783-1788 JJ denotes NF-κB
T7932 1789-1797 NN denotes enhancer
T7933 1798-1807 NNS denotes sequences
T7934 1808-1811 VBD denotes was
T7935 1812-1820 VBN denotes measured
T7936 1821-1823 IN denotes by
T7937 1824-1833 JJ denotes real-time
T7938 1834-1837 NNP denotes PCR
T7939 1838-1840 IN denotes of
T7940 1841-1850 NN denotes chromatin
T7941 1851-1870 NN denotes immunoprecipitation
T7942 1871-1872 -LRB- denotes (
T7943 1872-1876 NNP denotes ChIP
T7944 1876-1877 -RRB- denotes )
T7956 1940-1944 VBD denotes were
T7957 1945-1952 VBN denotes carried
T7958 1953-1956 RP denotes out
T7959 1957-1961 IN denotes with
T7960 1962-1965 DT denotes the
T7961 1966-1973 NNP denotes iCycler
T7962 1974-1976 NNP denotes iQ
T7963 1977-1986 NNP denotes Real-Time
T7964 1987-1996 NN denotes detection
T7965 1997-2003 NN denotes system
T7966 2004-2005 -LRB- denotes (
T7967 2005-2012 NNP denotes Bio-Rad
T7968 2013-2025 NNPS denotes Laboratories
T7969 2025-2026 -RRB- denotes )
T7970 2027-2032 VBG denotes using
T7971 2033-2036 DT denotes the
T7972 2037-2046 JJ denotes following
T7973 2047-2057 NNS denotes conditions
T7974 2057-2058 : denotes :
T7975 2059-2061 CD denotes 95
T7976 2061-2062 NN denotes °
T7977 2062-2063 NNP denotes C
T7978 2063-2064 , denotes ,
T7979 2065-2066 CD denotes 1
T7980 2067-2070 NN denotes min
T7981 2070-2071 : denotes ;
T7982 2072-2073 -LRB- denotes (
T7983 2073-2075 CD denotes 94
T7984 2075-2076 NN denotes °
T7985 2076-2077 NNP denotes C
T7986 2077-2078 , denotes ,
T7987 2079-2081 CD denotes 10
T7988 2082-2083 PRP denotes s
T7989 2083-2084 : denotes ;
T7990 2085-2087 CD denotes 60
T7991 2087-2088 NN denotes °
T7992 2088-2089 NNP denotes C
T7993 2089-2090 , denotes ,
T7994 2091-2093 CD denotes 30
T7995 2094-2095 PRP denotes s
T7996 2095-2096 -RRB- denotes )
T7997 2097-2098 RB denotes ×
T7998 2098-2100 CD denotes 40
T7999 2100-2101 . denotes .
T8000 2102-2109 NNS denotes Primers
T8001 2110-2114 VBD denotes used
T8002 2115-2118 IN denotes for
T8003 2119-2125 NNP denotes MIP-1α
T8004 2125-2126 , denotes ,
T8005 2127-2132 NNP denotes GAPDH
T8006 2133-2136 CC denotes and
T8007 2137-2141 NNP denotes ACTB
T8008 2142-2145 VBP denotes are
T8009 2146-2152 VBN denotes listed
T8010 2153-2155 IN denotes in
T8011 2156-2169 NNP denotes Supplementary
T8012 2170-2174 NNP denotes Data
T8013 2174-2175 . denotes .
T8014 2176-2185 JJ denotes Real-time
T8015 2186-2189 NNP denotes PCR
T8016 2190-2192 IN denotes of
T8017 2193-2197 NNP denotes CSF3
T8018 2197-2198 , denotes ,
T8019 2199-2202 NNP denotes LTA
T8020 2202-2203 , denotes ,
T8021 2204-2210 NNP denotes NFKBIA
T8022 2211-2214 CC denotes and
T8023 2215-2219 CD denotes TLR2
T8024 2220-2224 VBD denotes were
T8025 2225-2234 VBN denotes performed
T8026 2235-2240 VBG denotes using
T8027 2241-2244 DT denotes the
T8028 2245-2251 NNP denotes Custom
T8029 2252-2256 NNP denotes ChIP
T8030 2257-2262 NN denotes array
T8031 2263-2264 -LRB- denotes (
T8032 2264-2270 NNP denotes QIAGEN
T8033 2270-2271 -RRB- denotes )
T8034 2271-2272 . denotes .
T8035 2273-2276 IN denotes For
T8036 2277-2281 DT denotes each
T8037 2282-2288 NN denotes sample
T8038 2288-2289 , denotes ,
T8039 2290-2296 NNS denotes values
T8040 2297-2301 VBD denotes were
T8041 2302-2312 VBN denotes normalized
T8042 2313-2315 TO denotes to
T8043 2316-2321 NN denotes input
T8044 2322-2325 NN denotes DNA
T8045 2326-2329 CC denotes and
T8046 2330-2338 VBD denotes reported
T8047 2339-2341 IN denotes as
T8048 2342-2343 NN denotes %
T8049 2344-2346 IN denotes of
T8050 2347-2352 NN denotes input
T8051 2353-2357 IN denotes over
T8052 2358-2361 DT denotes the
T8053 2362-2368 NN denotes rabbit
T8054 2369-2372 NNP denotes IgG
T8055 2373-2380 NN denotes control
T8056 2380-2381 . denotes .
T7557 90-91 -RRB- denotes )
T7558 92-94 IN denotes at
T7559 95-98 DT denotes the
T7560 99-104 JJ denotes final
T7561 105-118 NN denotes concentration
T7562 119-121 IN denotes of
T7563 122-123 CD denotes 1
T7564 123-124 NN denotes %
T7565 124-125 . denotes .
T7566 126-131 IN denotes After
T7567 132-134 CD denotes 10
T7568 135-138 NN denotes min
T7569 138-139 , denotes ,
T7570 140-148 JJ denotes ice-cold
T7571 149-152 NNP denotes PBS
T7572 153-157 CC denotes plus
T7573 158-163 CD denotes 0.125
T7574 164-165 NNP denotes M
T7575 166-173 NN denotes glycine
T7576 174-177 VBD denotes was
T7577 178-183 VBN denotes added
T7578 183-184 , denotes ,
T7579 185-188 CC denotes and
T7580 189-195 NNS denotes plates
T7581 196-200 VBD denotes were
T7582 201-212 VBN denotes transferred
T7583 213-215 IN denotes on
T7584 216-219 NN denotes ice
T7585 219-220 , denotes ,
T7586 221-227 VBD denotes washed
T7587 228-239 RB denotes extensively
T7588 240-244 IN denotes with
T7880 1524-1526 NNP denotes mM
T7881 1527-1531 NNP denotes EDTA
T7882 1531-1532 -RRB- denotes )
T7883 1532-1533 , denotes ,
T7884 1534-1537 CC denotes and
T7885 1538-1547 VBN denotes extracted
T7886 1548-1550 IN denotes in
T7887 1551-1553 NNP denotes TE
T7888 1554-1560 NN denotes buffer
T7889 1561-1571 VBG denotes containing
T7890 1572-1573 CD denotes 2
T7891 1573-1574 NN denotes %
T7892 1575-1578 NNP denotes SDS
T7893 1578-1579 . denotes .
T7894 1580-1587 NN denotes Protein
T7895 1588-1591 NNP denotes DNA
T7896 1592-1603 NNS denotes cross-links
T7897 1604-1608 VBD denotes were
T7898 1609-1617 VBN denotes reverted
T7899 1618-1620 IN denotes by
T7900 1621-1628 NN denotes heating
T7901 1629-1631 IN denotes at
T7902 1632-1634 CD denotes 65
T7903 1634-1635 CD denotes °
T7904 1635-1636 NNP denotes C
T7905 1637-1646 JJ denotes overnight
T7906 1646-1647 . denotes .
T7907 1648-1651 NNP denotes DNA
T7908 1652-1655 VBD denotes was
T7909 1656-1663 JJ denotes further
T7910 1664-1672 VBN denotes purified
T7911 1673-1675 IN denotes by
T7912 1676-1684 NNP denotes QIAquick
T7913 1685-1688 NNP denotes PCR
T7914 1689-1701 NN denotes purification
T7945 1878-1885 VBZ denotes eluates
T7946 1886-1891 VBG denotes using
T7947 1892-1896 NNP denotes SYBR
T7948 1897-1904 NNP denotes GreenER
T7949 1905-1911 NNP denotes Master
T7950 1912-1915 NNP denotes Mix
T7951 1916-1917 -LRB- denotes (
T7952 1917-1927 NNP denotes Invitrogen
T7953 1927-1928 -RRB- denotes )
T7954 1928-1929 . denotes .
T7955 1930-1939 NNS denotes Reactions
R6267 T7546 T7549 amod Chromatin,Cells
R6268 T7547 T7549 compound immunoprecipitation,Cells
R6269 T7548 T7549 compound assay,Cells
R6270 T7549 T7551 nsubjpass Cells,fixed
R6271 T7550 T7551 auxpass were,fixed
R6272 T7551 T7551 ROOT fixed,fixed
R6273 T7552 T7551 prep by,fixed
R6274 T7553 T7552 pcomp adding,by
R6275 T7554 T7553 dobj formaldehyde,adding
R6276 T7555 T7556 punct (,Sigma-Aldrich
R6277 T7556 T7554 appos Sigma-Aldrich,formaldehyde
R6278 T7557 T7556 punct ),Sigma-Aldrich
R6279 T7558 T7553 prep at,adding
R6280 T7559 T7561 det the,concentration
R6281 T7560 T7561 amod final,concentration
R6282 T7561 T7558 pobj concentration,at
R6283 T7562 T7561 prep of,concentration
R6284 T7563 T7564 nummod 1,%
R6285 T7564 T7562 pobj %,of
R6286 T7565 T7551 punct .,fixed
R6287 T7566 T7577 prep After,added
R6288 T7567 T7568 nummod 10,min
R6289 T7568 T7566 pobj min,After
R6290 T7569 T7577 punct ",",added
R6291 T7570 T7571 amod ice-cold,PBS
R6292 T7571 T7577 nsubjpass PBS,added
R6293 T7572 T7571 cc plus,PBS
R6294 T7573 T7575 nummod 0.125,glycine
R6295 T7574 T7575 compound M,glycine
R6296 T7575 T7571 conj glycine,PBS
R6297 T7576 T7577 auxpass was,added
R6298 T7577 T7577 ROOT added,added
R6299 T7578 T7577 punct ",",added
R6300 T7579 T7577 cc and,added
R6301 T7580 T7582 nsubjpass plates,transferred
R6302 T7581 T7582 auxpass were,transferred
R6303 T7582 T7577 conj transferred,added
R6304 T7583 T7582 prep on,transferred
R6305 T7584 T7583 pobj ice,on
R6306 T7585 T7582 punct ",",transferred
R6307 T7586 T7582 conj washed,transferred
R6308 T7587 T7586 advmod extensively,washed
R6309 T7588 T7586 prep with,washed
R6310 T7589 T7588 pobj PBS,with
R6311 T7590 T7586 punct ",",washed
R6312 T7591 T7586 cc and,washed
R6313 T7592 T7586 conj scraped,washed
R6314 T7593 T7577 punct .,added
R6315 T7594 T7598 prep After,were
R6316 T7595 T7594 pobj centrifugation,After
R6317 T7596 T7598 punct ",",were
R6318 T7597 T7598 nsubj cells,were
R6319 T7598 T7620 ccomp were,supplemented
R6320 T7599 T7600 nummod 10,min
R6321 T7600 T7598 attr min,were
R6322 T7601 T7600 amod lysed,min
R6323 T7602 T7601 prep in,lysed
R6324 T7603 T7604 compound lysis,buffer
R6325 T7604 T7602 pobj buffer,in
R6326 T7605 T7620 punct (,supplemented
R6327 T7606 T7620 punct 5,supplemented
R6328 T7607 T7609 compound mM,pH
R6329 T7608 T7609 compound PIPES,pH
R6330 T7609 T7620 nsubj pH,supplemented
R6331 T7610 T7609 nummod 8.0,pH
R6332 T7611 T7609 punct ",",pH
R6333 T7612 T7614 nummod 85,KCl
R6334 T7613 T7614 compound mM,KCl
R6335 T7614 T7609 conj KCl,pH
R6336 T7615 T7614 punct ",",KCl
R6337 T7616 T7617 nummod 0.5,%
R6338 T7617 T7618 compound %,NP-40
R6339 T7618 T7614 appos NP-40,KCl
R6340 T7619 T7620 punct ),supplemented
R6341 T7620 T7620 ROOT supplemented,supplemented
R6342 T7621 T7620 prep with,supplemented
R6343 T7622 T7623 nummod 1,×
R6344 T7623 T7626 nummod ×,Inhibitor
R6345 T7624 T7626 amod Complete,Inhibitor
R6346 T7625 T7626 compound Protease,Inhibitor
R6347 T7626 T7621 pobj Inhibitor,with
R6348 T7627 T7630 punct (,GmbH
R6349 T7628 T7630 compound Roche,GmbH
R6350 T7629 T7630 compound Diagnostic,GmbH
R6351 T7630 T7626 appos GmbH,Inhibitor
R6352 T7631 T7630 punct ),GmbH
R6353 T7632 T7620 punct .,supplemented
R6354 T7633 T7635 nsubjpass Nuclei,pelletted
R6355 T7634 T7635 auxpass were,pelletted
R6356 T7635 T7635 ROOT pelletted,pelletted
R6357 T7636 T7635 punct (,pelletted
R6358 T7637 T7638 nummod 1000,×
R6359 T7638 T7639 compound ×,g
R6360 T7639 T7644 dep g,","
R6361 T7640 T7642 punct ",",min
R6362 T7641 T7642 nummod 5,min
R6363 T7642 T7639 appos min,g
R6364 T7643 T7639 punct ),g
R6365 T7644 T7635 punct ",",pelletted
R6366 T7645 T7635 cc and,pelletted
R6367 T7646 T7635 conj resuspended,pelletted
R6368 T7647 T7646 prep in,resuspended
R6369 T7648 T7649 compound sonication,buffer
R6370 T7649 T7647 pobj buffer,in
R6371 T7650 T7653 punct (,Tris
R6372 T7651 T7653 nummod 50,Tris
R6373 T7652 T7653 compound mM,Tris
R6374 T7653 T7646 dep Tris,resuspended
R6375 T7654 T7655 compound HCl,pH
R6376 T7655 T7653 appos pH,Tris
R6377 T7656 T7655 nummod 8.0,pH
R6378 T7657 T7655 punct ",",pH
R6379 T7658 T7659 nummod 1,%
R6380 T7659 T7660 compound %,SDS
R6381 T7660 T7655 appos SDS,pH
R6382 T7661 T7660 punct ",",SDS
R6383 T7662 T7664 nummod 10,EDTA
R6384 T7663 T7664 compound mM,EDTA
R6385 T7664 T7660 appos EDTA,SDS
R6386 T7665 T7660 punct ),SDS
R6387 T7666 T7653 punct .,Tris
R6388 T7667 T7669 nsubjpass Chromatin,sonicated
R6389 T7668 T7669 auxpass was,sonicated
R6390 T7669 T7669 ROOT sonicated,sonicated
R6391 T7670 T7669 advcl using,sonicated
R6392 T7671 T7673 compound Bandelin,GM70
R6393 T7672 T7673 compound Sonoplus,GM70
R6394 T7673 T7670 dobj GM70,using
R6395 T7674 T7673 punct (,GM70
R6396 T7675 T7676 compound Bandelin,Electronic
R6397 T7676 T7673 appos Electronic,GM70
R6398 T7677 T7676 punct ",",Electronic
R6399 T7678 T7676 conj Berlin,Electronic
R6400 T7679 T7678 punct ",",Berlin
R6401 T7680 T7678 conj Germany,Berlin
R6402 T7681 T7673 punct ),GM70
R6403 T7682 T7673 punct ",",GM70
R6404 T7683 T7673 amod centrifuged,GM70
R6405 T7684 T7696 punct (,was
R6406 T7685 T7686 nummod 14,000
R6407 T7686 T7688 nummod 000,g
R6408 T7687 T7688 compound ×,g
R6409 T7688 T7696 acomp g,was
R6410 T7689 T7691 punct ",",min
R6411 T7690 T7691 nummod 15,min
R6412 T7691 T7688 appos min,g
R6413 T7692 T7688 punct ),g
R6414 T7693 T7688 punct ",",g
R6415 T7694 T7688 cc and,g
R6416 T7695 T7696 nsubj supernatant,was
R6417 T7696 T7696 ROOT was,was
R6418 T7697 T7698 advmod 10-fold,diluted
R6419 T7698 T7696 acomp diluted,was
R6420 T7699 T7698 prep in,diluted
R6421 T7700 T7701 compound dilution,buffer
R6422 T7701 T7699 pobj buffer,in
R6423 T7702 T7705 punct (,SDS
R6424 T7703 T7704 nummod 0.01,%
R6425 T7704 T7705 npadvmod %,SDS
R6426 T7705 T7696 parataxis SDS,was
R6427 T7706 T7705 punct ",",SDS
R6428 T7707 T7709 nummod 16.7,Tris
R6429 T7708 T7709 compound mM,Tris
R6430 T7709 T7705 conj Tris,SDS
R6431 T7710 T7711 compound HCl,pH
R6432 T7711 T7705 npadvmod pH,SDS
R6433 T7712 T7711 nummod 8.0,pH
R6434 T7713 T7711 punct ",",pH
R6435 T7714 T7715 nummod 1.1,%
R6436 T7715 T7717 compound %,X-100
R6437 T7716 T7717 compound Triton,X-100
R6438 T7717 T7711 conj X-100,pH
R6439 T7718 T7717 punct ",",X-100
R6440 T7719 T7721 nummod 167,NaCl
R6441 T7720 T7721 compound mM,NaCl
R6442 T7721 T7717 conj NaCl,X-100
R6443 T7722 T7721 punct ",",NaCl
R6444 T7723 T7725 nummod 1.2,EDTA
R6445 T7724 T7725 compound mM,EDTA
R6446 T7725 T7721 appos EDTA,NaCl
R6447 T7726 T7721 punct ),NaCl
R6448 T7727 T7705 punct .,SDS
R6449 T7728 T7730 nsubjpass Samples,pre-cleared
R6450 T7729 T7730 auxpass were,pre-cleared
R6451 T7730 T7730 ROOT pre-cleared,pre-cleared
R6452 T7731 T7730 agent by,pre-cleared
R6453 T7732 T7733 amod 3-h,incubation
R6454 T7733 T7731 pobj incubation,by
R6455 T7734 T7730 prep with,pre-cleared
R6456 T7735 T7736 nummod 20,µl
R6457 T7736 T7734 pobj µl,with
R6458 T7737 T7736 prep of,µl
R6459 T7738 T7741 nmod protein,beads
R6460 T7739 T7741 nmod G,beads
R6461 T7740 T7741 amod agarose,beads
R6462 T7741 T7737 pobj beads,of
R6463 T7742 T7741 acl followed,beads
R6464 T7743 T7742 agent by,followed
R6465 T7744 T7743 pobj incubation,by
R6466 T7745 T7742 prep with,followed
R6467 T7746 T7745 pobj antibodies,with
R6468 T7747 T7746 prep against,antibodies
R6469 T7748 T7750 det the,proteins
R6470 T7749 T7750 amod analysed,proteins
R6471 T7750 T7747 pobj proteins,against
R6472 T7751 T7730 punct .,pre-cleared
R6473 T7752 T7753 amod Primary,antibodies
R6474 T7753 T7754 nsubj antibodies,were
R6475 T7754 T7754 ROOT were,were
R6476 T7755 T7754 punct :,were
R6477 T7756 T7767 amod anti-p65,IgG
R6478 T7757 T7767 punct (,IgG
R6479 T7758 T7767 amod sc-372,IgG
R6480 T7759 T7758 punct ),sc-372
R6481 T7760 T7758 punct ",",sc-372
R6482 T7761 T7758 conj anti-IκBα,sc-372
R6483 T7762 T7763 punct (,sc-203
R6484 T7763 T7767 amod sc-203,IgG
R6485 T7764 T7763 punct ),sc-203
R6486 T7765 T7763 cc and,sc-203
R6487 T7766 T7763 conj rabbit,sc-203
R6488 T7767 T7754 attr IgG,were
R6489 T7768 T7769 punct (,sc-2027
R6490 T7769 T7767 appos sc-2027,IgG
R6491 T7770 T7769 punct ),sc-2027
R6492 T7771 T7769 prep from,sc-2027
R6493 T7772 T7773 compound Santa,Cruz
R6494 T7773 T7774 compound Cruz,Biotechnology
R6495 T7774 T7771 pobj Biotechnology,from
R6496 T7775 T7767 punct ;,IgG
R6497 T7776 T7777 compound anti-FLAG,M2
R6498 T7777 T7767 conj M2,IgG
R6499 T7778 T7767 prep from,IgG
R6500 T7779 T7778 pobj Sigma,from
R6501 T7780 T7767 conj Aldrich,IgG
R6502 T7781 T7767 punct .,IgG
R6503 T7782 T7784 nsubjpass Immunoprecipitations,carried
R6504 T7783 T7784 auxpass were,carried
R6505 T7784 T7784 ROOT carried,carried
R6506 T7785 T7784 prt out,carried
R6507 T7786 T7784 prep at,carried
R6508 T7787 T7788 nummod 4,°
R6509 T7788 T7790 nummod °,overnight
R6510 T7789 T7790 compound C,overnight
R6511 T7790 T7786 pobj overnight,at
R6512 T7791 T7790 cc and,overnight
R6513 T7792 T7790 conj immune,overnight
R6514 T7793 T7795 nsubjpass complexes,collected
R6515 T7794 T7795 auxpass were,collected
R6516 T7795 T7784 conj collected,carried
R6517 T7796 T7795 prep with,collected
R6518 T7797 T7800 nmod protein,beads
R6519 T7798 T7800 nmod G,beads
R6520 T7799 T7800 amod agarose,beads
R6521 T7800 T7796 pobj beads,with
R6522 T7801 T7802 punct ",",washed
R6523 T7802 T7784 conj washed,carried
R6524 T7803 T7804 nummod five,times
R6525 T7804 T7802 npadvmod times,washed
R6526 T7805 T7802 prep with,washed
R6527 T7806 T7808 amod low,buffer
R6528 T7807 T7808 compound salt,buffer
R6529 T7808 T7805 pobj buffer,with
R6530 T7809 T7808 punct (,buffer
R6531 T7810 T7812 nummod 20,Tris
R6532 T7811 T7812 compound mM,Tris
R6533 T7812 T7808 appos Tris,buffer
R6534 T7813 T7814 compound HCl,pH
R6535 T7814 T7808 appos pH,buffer
R6536 T7815 T7814 nummod 8.0,pH
R6537 T7816 T7814 punct ",",pH
R6538 T7817 T7818 nummod 0.1,%
R6539 T7818 T7819 npadvmod %,SDS
R6540 T7819 T7814 appos SDS,pH
R6541 T7820 T7819 punct ",",SDS
R6542 T7821 T7822 nummod 1,%
R6543 T7822 T7824 compound %,X-100
R6544 T7823 T7824 compound Triton,X-100
R6545 T7824 T7819 conj X-100,SDS
R6546 T7825 T7824 punct ",",X-100
R6547 T7826 T7828 nummod 2,EDTA
R6548 T7827 T7828 compound mM,EDTA
R6549 T7828 T7824 conj EDTA,X-100
R6550 T7829 T7828 punct ",",EDTA
R6551 T7830 T7832 nummod 150,NaCl
R6552 T7831 T7832 compound mM,NaCl
R6553 T7832 T7828 appos NaCl,EDTA
R6554 T7833 T7828 punct ),EDTA
R6555 T7834 T7819 punct ",",SDS
R6556 T7835 T7836 nummod four,times
R6557 T7836 T7819 npadvmod times,SDS
R6558 T7837 T7819 prep with,SDS
R6559 T7838 T7840 amod high,buffer
R6560 T7839 T7840 compound salt,buffer
R6561 T7840 T7837 pobj buffer,with
R6562 T7841 T7844 punct (,Tris
R6563 T7842 T7844 nummod 20,Tris
R6564 T7843 T7844 compound mM,Tris
R6565 T7844 T7840 appos Tris,buffer
R6566 T7845 T7846 compound HCl,pH
R6567 T7846 T7885 nsubj pH,extracted
R6568 T7847 T7846 nummod 8.0,pH
R6569 T7848 T7846 punct ",",pH
R6570 T7849 T7850 nummod 0.1,%
R6571 T7850 T7851 npadvmod %,SDS
R6572 T7851 T7846 npadvmod SDS,pH
R6573 T7852 T7851 punct ",",SDS
R6574 T7853 T7854 nummod 1,%
R6575 T7854 T7856 compound %,X-100
R6576 T7855 T7856 compound Triton,X-100
R6577 T7856 T7851 appos X-100,SDS
R6578 T7857 T7856 punct ",",X-100
R6579 T7858 T7860 nummod 2,EDTA
R6580 T7859 T7860 compound mM,EDTA
R6581 T7860 T7856 conj EDTA,X-100
R6582 T7861 T7860 punct ",",EDTA
R6583 T7862 T7864 nummod 500,NaCl
R6584 T7863 T7864 compound mM,NaCl
R6585 T7864 T7860 appos NaCl,EDTA
R6586 T7865 T7860 punct ),EDTA
R6587 T7866 T7851 punct ",",SDS
R6588 T7867 T7868 advmod once,with
R6589 T7868 T7851 prep with,SDS
R6590 T7869 T7870 compound TE,buffer
R6591 T7870 T7868 pobj buffer,with
R6592 T7871 T7851 punct (,SDS
R6593 T7872 T7874 nummod 10,Tris
R6594 T7873 T7874 compound mM,Tris
R6595 T7874 T7851 appos Tris,SDS
R6596 T7875 T7876 compound HCl,pH
R6597 T7876 T7851 appos pH,SDS
R6598 T7877 T7876 nummod 8.0,pH
R6599 T7878 T7876 punct ",",pH
R6600 T7879 T7881 nummod 1,EDTA
R6601 T7880 T7881 compound mM,EDTA
R6602 T7881 T7876 appos EDTA,pH
R6603 T7882 T7876 punct ),pH
R6604 T7883 T7851 punct ",",SDS
R6605 T7884 T7846 cc and,pH
R6606 T7885 T7885 ROOT extracted,extracted
R6607 T7886 T7885 prep in,extracted
R6608 T7887 T7888 compound TE,buffer
R6609 T7888 T7886 pobj buffer,in
R6610 T7889 T7888 acl containing,buffer
R6611 T7890 T7891 nummod 2,%
R6612 T7891 T7889 dobj %,containing
R6613 T7892 T7889 npadvmod SDS,containing
R6614 T7893 T7885 punct .,extracted
R6615 T7894 T7894 ROOT Protein,Protein
R6616 T7895 T7896 compound DNA,cross-links
R6617 T7896 T7898 nsubjpass cross-links,reverted
R6618 T7897 T7898 auxpass were,reverted
R6619 T7898 T7898 ROOT reverted,reverted
R6620 T7899 T7898 agent by,reverted
R6621 T7900 T7899 pobj heating,by
R6622 T7901 T7898 prep at,reverted
R6623 T7902 T7904 nummod 65,C
R6624 T7903 T7904 nummod °,C
R6625 T7904 T7905 compound C,overnight
R6626 T7905 T7901 pobj overnight,at
R6627 T7906 T7898 punct .,reverted
R6628 T7907 T7910 nsubjpass DNA,purified
R6629 T7908 T7910 auxpass was,purified
R6630 T7909 T7910 advmod further,purified
R6631 T7910 T7910 ROOT purified,purified
R6650 T7929 T7935 nsubjpass enrichment,measured
R6651 T7930 T7929 prep in,enrichment
R6652 T7931 T7933 amod NF-κB,sequences
R6653 T7932 T7933 compound enhancer,sequences
R6654 T7933 T7930 pobj sequences,in
R6655 T7934 T7935 auxpass was,measured
R6656 T7935 T7935 ROOT measured,measured
R6657 T7936 T7935 agent by,measured
R6658 T7937 T7938 amod real-time,PCR
R6659 T7938 T7936 pobj PCR,by
R6660 T7939 T7938 prep of,PCR
R6661 T7940 T7941 compound chromatin,immunoprecipitation
R6662 T7941 T7939 pobj immunoprecipitation,of
R6663 T7942 T7943 punct (,ChIP
R6664 T7943 T7941 appos ChIP,immunoprecipitation
R6665 T7944 T7941 punct ),immunoprecipitation
R6666 T7945 T7935 oprd eluates,measured
R6667 T7946 T7945 advcl using,eluates
R6668 T7947 T7950 compound SYBR,Mix
R6669 T7948 T7950 compound GreenER,Mix
R6670 T7949 T7950 compound Master,Mix
R6671 T7950 T7946 dobj Mix,using
R6672 T7951 T7950 punct (,Mix
R6673 T7952 T7950 appos Invitrogen,Mix
R6674 T7953 T7950 punct ),Mix
R6675 T7954 T7935 punct .,measured
R6676 T7955 T7957 nsubjpass Reactions,carried
R6677 T7956 T7957 auxpass were,carried
R6678 T7957 T7957 ROOT carried,carried
R6679 T7958 T7957 prt out,carried
R6680 T7959 T7957 prep with,carried
R6681 T7960 T7965 det the,system
R6682 T7961 T7963 compound iCycler,Real-Time
R6683 T7962 T7963 compound iQ,Real-Time
R6684 T7963 T7965 compound Real-Time,system
R6685 T7964 T7965 compound detection,system
R6686 T7965 T7959 pobj system,with
R6687 T7966 T7965 punct (,system
R6688 T7967 T7968 compound Bio-Rad,Laboratories
R6689 T7968 T7965 appos Laboratories,system
R6690 T7969 T7965 punct ),system
R6691 T7970 T7957 advcl using,carried
R6692 T7971 T7973 det the,conditions
R6693 T7972 T7973 amod following,conditions
R6694 T7973 T7970 dobj conditions,using
R6695 T7974 T7973 punct :,conditions
R6696 T7975 T7976 nummod 95,°
R6697 T7976 T7977 compound °,C
R6698 T7977 T7973 appos C,conditions
R6699 T7978 T7977 punct ",",C
R6700 T7979 T7980 nummod 1,min
R6701 T7980 T7977 appos min,C
R6702 T7981 T7977 punct ;,C
R6703 T7982 T7985 punct (,C
R6704 T7983 T7984 nummod 94,°
R6705 T7984 T7985 compound °,C
R6706 T7985 T7977 appos C,C
R6707 T7986 T7985 punct ",",C
R6708 T7987 T7988 nsubj 10,s
R6709 T7988 T7977 appos s,C
R6710 T7989 T7977 punct ;,C
R6711 T7990 T7991 nummod 60,°
R6712 T7991 T7992 compound °,C
R6713 T7992 T7977 conj C,C
R6714 T7993 T7992 punct ",",C
R6715 T7994 T7995 nummod 30,s
R6716 T7995 T7992 appos s,C
R6717 T7996 T7992 punct ),C
R6718 T7997 T7998 advmod ×,40
R6719 T7998 T7977 appos 40,C
R6720 T7999 T7957 punct .,carried
R6721 T8000 T8009 nsubjpass Primers,listed
R6722 T8001 T8000 acl used,Primers
R6723 T8002 T8001 prep for,used
R6724 T8003 T8002 pobj MIP-1α,for
R6725 T8004 T8003 punct ",",MIP-1α
R6726 T8005 T8003 conj GAPDH,MIP-1α
R6727 T8006 T8005 cc and,GAPDH
R6728 T8007 T8005 conj ACTB,GAPDH
R6729 T8008 T8009 auxpass are,listed
R6730 T8009 T8009 ROOT listed,listed
R6731 T8010 T8009 prep in,listed
R6732 T8011 T8012 compound Supplementary,Data
R6733 T8012 T8010 pobj Data,in
R6734 T8013 T8009 punct .,listed
R6735 T8014 T8015 amod Real-time,PCR
R6736 T8015 T8025 nsubjpass PCR,performed
R6737 T8016 T8015 prep of,PCR
R6738 T8017 T8016 pobj CSF3,of
R6739 T8018 T8017 punct ",",CSF3
R6740 T8019 T8017 conj LTA,CSF3
R6741 T8020 T8019 punct ",",LTA
R6742 T8021 T8019 conj NFKBIA,LTA
R6743 T8022 T8021 cc and,NFKBIA
R6744 T8023 T8021 conj TLR2,NFKBIA
R6745 T8024 T8025 auxpass were,performed
R6746 T8025 T8025 ROOT performed,performed
R6747 T8026 T8025 advcl using,performed
R6748 T8027 T8030 det the,array
R6749 T8028 T8030 compound Custom,array
R6750 T8029 T8030 compound ChIP,array
R6751 T8030 T8026 dobj array,using
R6752 T8031 T8030 punct (,array
R6753 T8032 T8030 appos QIAGEN,array
R6754 T8033 T8030 punct ),array
R6755 T8034 T8025 punct .,performed
R6756 T8035 T8040 prep For,were
R6757 T8036 T8037 det each,sample
R6758 T8037 T8035 pobj sample,For
R6759 T8038 T8040 punct ",",were
R6760 T8039 T8040 nsubj values,were
R6761 T8040 T8040 ROOT were,were
R6762 T8041 T8040 acomp normalized,were
R6763 T8042 T8041 prep to,normalized
R6764 T8043 T8046 dep input,reported
R6765 T8044 T8043 dobj DNA,input
R6766 T8045 T8043 cc and,input
R6767 T8046 T8040 conj reported,were
R6768 T8047 T8048 mark as,%
R6769 T8048 T8046 advcl %,reported
R6770 T8049 T8048 prep of,%
R6771 T8050 T8049 pobj input,of
R6772 T8051 T8048 prep over,%
R6773 T8052 T8053 det the,rabbit
R6774 T8053 T8051 pobj rabbit,over
R6775 T8054 T8055 compound IgG,control
R6776 T8055 T8048 conj control,%
R6777 T8056 T8046 punct .,reported
R6632 T7911 T7910 agent by,purified
R6633 T7912 T7915 compound QIAquick,kit
R6634 T7913 T7915 compound PCR,kit
R6635 T7914 T7915 compound purification,kit
R6636 T7915 T7911 pobj kit,by
R6637 T7916 T7915 punct (,kit
R6638 T7917 T7915 appos QIAGEN,kit
R6639 T7918 T7915 punct ),kit
R6640 T7919 T7910 cc and,purified
R6641 T7920 T7910 conj eluted,purified
R6642 T7921 T7920 prep in,eluted
R6643 T7922 T7923 nummod 50,µl
R6644 T7923 T7924 npadvmod µl,sterile
R6645 T7924 T7926 amod sterile,water
R6646 T7925 T7926 compound distilled,water
R6647 T7926 T7921 pobj water,in
R6648 T7927 T7910 punct .,purified
R6649 T7928 T7929 amod Specific,enrichment

UBERON-AE

Id Subject Object Predicate Lexical cue
T7505 228-239 http://purl.obolibrary.org/obo/UBERON_2000106 denotes extensively

GO-BP

Id Subject Object Predicate Lexical cue
T8080 312-317 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

GO-MF

Id Subject Object Predicate Lexical cue
T8081 939-949 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T8082 989-999 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T8072 0-9 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T8073 565-574 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T8074 1841-1850 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T8075 285-290 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8076 939-949 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T8077 989-999 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T8078 939-949 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T8079 989-999 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T7515 0-35 Sentence denotes Chromatin immunoprecipitation assay
T7516 36-125 Sentence denotes Cells were fixed by adding formaldehyde (Sigma-Aldrich) at the final concentration of 1%.
T7517 126-262 Sentence denotes After 10 min, ice-cold PBS plus 0.125 M glycine was added, and plates were transferred on ice, washed extensively with PBS, and scraped.
T7518 263-441 Sentence denotes After centrifugation, cells were 10 min lysed in lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40) supplemented with 1× Complete Protease Inhibitor (Roche Diagnostic GmbH).
T7519 442-564 Sentence denotes Nuclei were pelletted (1000 × g, 5 min), and resuspended in sonication buffer (50 mM Tris–HCl pH 8.0, 1% SDS, 10 mM EDTA).
T7520 565-829 Sentence denotes Chromatin was sonicated using Bandelin Sonoplus GM70 (Bandelin Electronic, Berlin, Germany), centrifuged (14 000 × g, 15 min), and supernatant was 10-fold diluted in dilution buffer (0.01% SDS, 16.7 mM Tris–HCl pH 8.0, 1.1% Triton X-100, 167 mM NaCl, 1.2 mM EDTA).
T7521 830-980 Sentence denotes Samples were pre-cleared by 3-h incubation with 20 µl of protein G agarose beads followed by incubation with antibodies against the analysed proteins.
T7522 981-1579 Sentence denotes Primary antibodies were: anti-p65 (sc-372), anti-IκBα (sc-203) and rabbit IgG (sc-2027) from Santa Cruz Biotechnology; anti-FLAG M2 from Sigma–Aldrich. Immunoprecipitations were carried out at 4°C overnight and immune complexes were collected with protein G agarose beads, washed five times with low salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl), four times with high salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 500 mM NaCl), once with TE buffer (10 mM Tris–HCl pH 8.0, 1 mM EDTA), and extracted in TE buffer containing 2% SDS.
T7523 1580-1647 Sentence denotes Protein–DNA cross-links were reverted by heating at 65°C overnight.
T7524 1648-1759 Sentence denotes DNA was further purified by QIAquick PCR purification kit (QIAGEN) and eluted in 50 µl sterile distilled water.
T7525 1760-1929 Sentence denotes Specific enrichment in NF-κB enhancer sequences was measured by real-time PCR of chromatin immunoprecipitation (ChIP) eluates using SYBR GreenER Master Mix (Invitrogen).
T7526 1930-2058 Sentence denotes Reactions were carried out with the iCycler iQ Real-Time detection system (Bio-Rad Laboratories) using the following conditions:
T7527 2059-2101 Sentence denotes 95°C, 1 min; (94°C, 10 s; 60°C, 30 s) ×40.
T7528 2102-2272 Sentence denotes Primers used for MIP-1α, GAPDH and ACTB are listed in Supplementary Data. Real-time PCR of CSF3, LTA, NFKBIA and TLR2 were performed using the Custom ChIP array (QIAGEN).
T7529 2273-2381 Sentence denotes For each sample, values were normalized to input DNA and reported as % of input over the rabbit IgG control.
T83 0-35 Sentence denotes Chromatin immunoprecipitation assay
T84 36-125 Sentence denotes Cells were fixed by adding formaldehyde (Sigma-Aldrich) at the final concentration of 1%.
T85 126-262 Sentence denotes After 10 min, ice-cold PBS plus 0.125 M glycine was added, and plates were transferred on ice, washed extensively with PBS, and scraped.
T86 263-441 Sentence denotes After centrifugation, cells were 10 min lysed in lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40) supplemented with 1× Complete Protease Inhibitor (Roche Diagnostic GmbH).
T87 442-564 Sentence denotes Nuclei were pelletted (1000 × g, 5 min), and resuspended in sonication buffer (50 mM Tris–HCl pH 8.0, 1% SDS, 10 mM EDTA).
T88 565-829 Sentence denotes Chromatin was sonicated using Bandelin Sonoplus GM70 (Bandelin Electronic, Berlin, Germany), centrifuged (14 000 × g, 15 min), and supernatant was 10-fold diluted in dilution buffer (0.01% SDS, 16.7 mM Tris–HCl pH 8.0, 1.1% Triton X-100, 167 mM NaCl, 1.2 mM EDTA).
T89 830-980 Sentence denotes Samples were pre-cleared by 3-h incubation with 20 µl of protein G agarose beads followed by incubation with antibodies against the analysed proteins.
T90 981-1132 Sentence denotes Primary antibodies were: anti-p65 (sc-372), anti-IκBα (sc-203) and rabbit IgG (sc-2027) from Santa Cruz Biotechnology; anti-FLAG M2 from Sigma–Aldrich.
T91 1133-1579 Sentence denotes Immunoprecipitations were carried out at 4°C overnight and immune complexes were collected with protein G agarose beads, washed five times with low salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl), four times with high salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 500 mM NaCl), once with TE buffer (10 mM Tris–HCl pH 8.0, 1 mM EDTA), and extracted in TE buffer containing 2% SDS.
T92 1580-1647 Sentence denotes Protein–DNA cross-links were reverted by heating at 65°C overnight.
T93 1648-1759 Sentence denotes DNA was further purified by QIAquick PCR purification kit (QIAGEN) and eluted in 50 µl sterile distilled water.
T94 1760-1929 Sentence denotes Specific enrichment in NF-κB enhancer sequences was measured by real-time PCR of chromatin immunoprecipitation (ChIP) eluates using SYBR GreenER Master Mix (Invitrogen).
T95 1930-2058 Sentence denotes Reactions were carried out with the iCycler iQ Real-Time detection system (Bio-Rad Laboratories) using the following conditions:
T96 2059-2101 Sentence denotes 95°C, 1 min; (94°C, 10 s; 60°C, 30 s) ×40.
T97 2102-2175 Sentence denotes Primers used for MIP-1α, GAPDH and ACTB are listed in Supplementary Data.
T98 2176-2272 Sentence denotes Real-time PCR of CSF3, LTA, NFKBIA and TLR2 were performed using the Custom ChIP array (QIAGEN).
T99 2273-2381 Sentence denotes For each sample, values were normalized to input DNA and reported as % of input over the rabbit IgG control.

events-check-again

Id Subject Object Predicate Lexical cue
T8128 887-896 Protein denotes protein G
T8129 1229-1238 Protein denotes protein G
T8130 2119-2125 Protein denotes MIP-1α
T8131 2127-2132 Protein denotes GAPDH
T8132 2137-2141 Protein denotes ACTB
T8133 2193-2197 Protein denotes CSF3
T8134 2199-2202 Protein denotes LTA
T8135 2204-2210 Protein denotes NFKBIA
T8136 2215-2219 Protein denotes TLR2

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T8137 0-9 Protein denotes Chromatin
T8138 386-406 Protein denotes 1× Complete Protease
T8139 407-416 Negative_regulation denotes Inhibitor
T8140 565-574 Protein denotes Chromatin
T8141 1011-1014 Protein denotes p65
T8142 1025-1043 Protein denotes anti-IκBα (sc-203)
T8143 1055-1058 Protein denotes IgG
T8144 1783-1797 Protein denotes NF-κB enhancer
T8145 1892-1915 Protein denotes SYBR GreenER Master Mix
T8146 2119-2125 Protein denotes MIP-1α
T8147 2127-2132 Protein denotes GAPDH
T8148 2137-2141 Protein denotes ACTB
T8149 2193-2197 Protein denotes CSF3
T8150 2199-2202 Protein denotes LTA
T8151 2204-2210 Protein denotes NFKBIA
T8152 2215-2219 Protein denotes TLR2
T8153 2369-2372 Protein denotes IgG
R6778 T8138 T8139 themeOf 1× Complete Protease,Inhibitor

test2

Id Subject Object Predicate Lexical cue
T7496 887-896 Protein denotes protein G
T7497 1229-1238 Protein denotes protein G
T7498 2119-2125 Protein denotes MIP-1α
T7499 2127-2132 Protein denotes GAPDH
T7500 2137-2141 Protein denotes ACTB
T7501 2193-2197 Protein denotes CSF3
T7502 2199-2202 Protein denotes LTA
T7503 2204-2210 Protein denotes NFKBIA
T7504 2215-2219 Protein denotes TLR2

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T7506 887-896 Protein denotes protein G
T7507 1229-1238 Protein denotes protein G
T7508 2119-2125 Protein denotes MIP-1α
T7509 2127-2132 Protein denotes GAPDH
T7510 2137-2141 Protein denotes ACTB
T7511 2193-2197 Protein denotes CSF3
T7512 2199-2202 Protein denotes LTA
T7513 2204-2210 Protein denotes NFKBIA
T7514 2215-2219 Protein denotes TLR2

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T7530 337-339 P0A7Z4 denotes pH
T7531 536-538 P0A7Z4 denotes pH
T7532 776-778 P0A7Z4 denotes pH
T7533 1011-1014 Q04206 denotes p65
T7534 1011-1014 P21579 denotes p65
T7535 1030-1034 P25963 denotes IκBα
T7536 1309-1311 P0A7Z4 denotes pH
T7537 1418-1420 P0A7Z4 denotes pH
T7538 1514-1516 P0A7Z4 denotes pH
T7539 2119-2125 P10147 denotes MIP-1α
T7540 2127-2132 P04406 denotes GAPDH
T7541 2137-2141 P60709 denotes ACTB
T7542 2193-2197 P09919 denotes CSF3
T7543 2199-2202 P01374 denotes LTA
T7544 2204-2210 P25963 denotes NFKBIA
T7545 2215-2219 O60603 denotes TLR2